FastQCFastQC Report
Thu 26 May 2016
SRR937668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041670
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57580.5527662311480603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.4211506523179126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28590.2744631217180105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13550.13007958374533202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.10559966208108133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.035.7184681
GTATCAA57900.034.8967251
ATCAACG71900.027.95883
TCAACGC72900.027.4501824
CAACGCA73200.027.337685
TATCAAC73650.027.2299422
ATACCGA700.00838882427.1444366
AACGCAG74650.026.9171736
GTCGGGA1201.7049714E-423.7616752
CGCAGAG84750.023.2048918
ACGCAGA85350.023.1530767
TACATGG46850.022.9248182
GTACATG49400.022.5197321
GTATAGG2554.8567017E-1022.3725451
TGTATCG1703.1469135E-622.3542449
GAGTACT47200.022.19037412-13
ACATGGG47900.021.9262223
GCAGAGT88550.021.8335699
CCCTATA2005.3596705E-721.3855082
AGTACAT37550.021.135552
Produced by FastQC (version 0.11.5)