##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1041670 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.976505035183887 33.0 31.0 34.0 31.0 34.0 2 32.0918669060259 33.0 31.0 34.0 30.0 34.0 3 32.1284744688817 34.0 31.0 34.0 30.0 34.0 4 35.72613783635892 37.0 35.0 37.0 35.0 37.0 5 35.73737940038592 37.0 35.0 37.0 35.0 37.0 6 35.81043996659211 37.0 35.0 37.0 35.0 37.0 7 35.7430817821383 37.0 35.0 37.0 35.0 37.0 8 35.745604654065104 37.0 35.0 37.0 35.0 37.0 9 37.33842195704974 39.0 37.0 39.0 35.0 39.0 10-11 37.289805312623 39.0 37.0 39.0 34.5 39.0 12-13 37.205170063455796 39.0 37.0 39.0 34.0 39.0 14-15 38.37355976460876 40.0 38.0 41.0 34.0 41.0 16-17 38.28866147628328 40.0 38.0 41.0 34.0 41.0 18-19 38.25008255973581 40.0 38.0 41.0 34.0 41.0 20-21 38.27207944934577 40.0 38.0 41.0 34.0 41.0 22-23 38.27172473048086 40.0 38.0 41.0 34.0 41.0 24-25 38.211127324392564 40.0 38.0 41.0 34.0 41.0 26-27 38.10975212879319 40.0 38.0 41.0 34.0 41.0 28-29 37.96743258421573 40.0 38.0 41.0 33.5 41.0 30-31 37.72097977286472 40.0 37.5 41.0 33.0 41.0 32-33 37.56749882400376 40.0 37.0 41.0 33.0 41.0 34-35 37.537939558593415 40.0 37.0 41.0 33.0 41.0 36-37 37.37923622644408 40.0 37.0 41.0 33.0 41.0 38-39 37.208485412846684 40.0 37.0 41.0 32.0 41.0 40-41 36.98429589025315 40.0 36.5 41.0 31.5 41.0 42-43 36.705753261589564 39.0 36.0 41.0 31.0 41.0 44-45 36.412936438603396 39.0 36.0 41.0 30.0 41.0 46-47 35.985890445150574 39.0 35.5 41.0 29.5 41.0 48-49 35.58969107298857 39.0 35.0 40.0 27.5 41.0 50-51 34.716733706452146 38.0 34.0 39.5 26.0 40.5 52-53 34.8228484068851 38.0 35.0 40.0 26.0 40.5 54-55 35.09543041462267 39.0 35.0 40.0 26.0 41.0 56-57 34.875161039484674 38.5 35.0 40.0 25.5 41.0 58-59 34.613770675933836 38.0 34.0 40.0 24.5 41.0 60-61 34.27983046454251 38.0 34.0 40.0 23.0 41.0 62-63 33.9258047174249 38.0 34.0 40.0 22.5 41.0 64-65 33.505923181045816 37.0 33.0 40.0 21.5 41.0 66-67 32.980022943926585 36.5 32.5 39.0 18.0 41.0 68-69 32.53346693290581 36.0 32.0 39.0 17.5 40.0 70-71 32.063784115890826 35.5 31.5 39.0 13.0 40.0 72-73 31.58419893056342 35.0 31.0 38.0 8.0 40.0 74-75 31.040043391861147 35.0 30.5 37.0 7.0 39.0 76-77 30.49145938732996 34.5 30.0 37.0 2.0 39.0 78-79 29.962030201503353 34.0 29.5 36.0 2.0 39.0 80-81 29.41394251538395 34.0 29.0 36.0 2.0 37.5 82-83 28.87245240815229 34.0 29.0 35.0 2.0 37.0 84-85 28.31814442193785 34.0 27.5 35.0 2.0 36.5 86-87 27.731704858544454 33.0 26.0 35.0 2.0 36.0 88-89 27.18526020716734 33.0 26.0 35.0 2.0 36.0 90-91 26.61674042643064 32.5 25.0 35.0 2.0 35.0 92-93 25.95793197461768 32.0 23.5 34.5 2.0 35.0 94-95 25.201339675713037 32.0 19.5 34.0 2.0 35.0 96-97 24.33083798131846 31.0 12.5 34.0 2.0 35.0 98-99 23.137435560206207 31.0 2.0 34.0 2.0 35.0 100-101 19.8781077500552 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15636928088953184 1101 2 0.04473508171491858 1101 3 -0.016411088872338553 1101 4 0.11915399895305967 1101 5 0.11933168282075712 1101 6 0.15759844111875765 1101 7 0.19929430970469042 1101 8 0.20149673542681512 1101 9 0.31623259853059693 1101 10-11 0.27388580933446605 1101 12-13 0.3488532068414827 1101 14-15 0.5524013147759206 1101 16-17 0.4029961384352916 1101 18-19 0.5533121173014379 1101 20-21 0.4044589811834456 1101 22-23 0.3738282166949105 1101 24-25 0.30525455161420467 1101 26-27 0.4827124912196723 1101 28-29 0.3014230531300157 1101 30-31 0.45742868678573956 1101 32-33 0.460321354373292 1101 34-35 0.5470431899109869 1101 36-37 0.7488934287882074 1101 38-39 0.46416512057520265 1101 40-41 0.6384702756001133 1101 42-43 0.6198040391619628 1101 44-45 0.8448042358476329 1101 46-47 0.9238504755143495 1101 48-49 0.8761183581042786 1101 50-51 0.6993116554495913 1101 52-53 0.9925421645793833 1101 54-55 1.1140091029344958 1101 56-57 1.186672609970195 1101 58-59 1.2704618455714964 1101 60-61 1.241468216181552 1101 62-63 1.1550035162627168 1101 64-65 1.3034273571652761 1101 66-67 1.2494085238364576 1101 68-69 1.4248183052772632 1101 70-71 1.2766542149527602 1101 72-73 1.4488610762584138 1101 74-75 1.3151845844197503 1101 76-77 1.1567863032392225 1101 78-79 1.3559435145965608 1101 80-81 1.7039443410606907 1101 82-83 1.5171044415805497 1101 84-85 1.8326840553795982 1101 86-87 1.8511699900432994 1101 88-89 1.9388539576218164 1101 90-91 1.8727241124546765 1101 92-93 1.9906195580012174 1101 94-95 2.4599672381036193 1101 96-97 2.2536757100622644 1101 98-99 2.7393349511717986 1101 100-101 3.452910499809491 1102 1 0.20965027852315288 1102 2 0.1919655466011676 1102 3 0.23380864594341944 1102 4 0.2291666446604026 1102 5 0.1143917186293919 1102 6 0.08058983953365839 1102 7 0.20394630234263644 1102 8 0.18530271772282703 1102 9 0.2336655199394002 1102 10-11 0.28437673823053444 1102 12-13 0.20438006957921573 1102 14-15 0.3476532272826489 1102 16-17 0.4220285540158031 1102 18-19 0.31091268823634977 1102 20-21 0.2996392138143804 1102 22-23 0.24820219157877688 1102 24-25 0.2810784006670275 1102 26-27 0.3408924483995577 1102 28-29 0.2654588492461585 1102 30-31 0.4002979477004871 1102 32-33 0.4323380316823773 1102 34-35 0.5229512393419284 1102 36-37 0.5732282771023236 1102 38-39 0.612901175865737 1102 40-41 0.6448835899022853 1102 42-43 0.7148853598245424 1102 44-45 0.6520207403737999 1102 46-47 0.9321017995574721 1102 48-49 0.9960325300608517 1102 50-51 0.8214854650628283 1102 52-53 0.8430436106974 1102 54-55 0.8807008106058021 1102 56-57 0.881451560501695 1102 58-59 0.9803605547077083 1102 60-61 0.9467787872416622 1102 62-63 0.9417421132152342 1102 64-65 0.9781957278723041 1102 66-67 1.1590142532183236 1102 68-69 1.2679335940626544 1102 70-71 1.237039345604206 1102 72-73 1.4184760082094847 1102 74-75 1.6030647381715148 1102 76-77 1.268211261608549 1102 78-79 1.7477256828971548 1102 80-81 1.894157377407712 1102 82-83 1.9349051527450811 1102 84-85 2.1085899137226356 1102 86-87 2.2552269458083636 1102 88-89 2.1912218976235707 1102 90-91 2.2912697935074 1102 92-93 2.2802716758441868 1102 94-95 2.0713048659692497 1102 96-97 2.19299196543043 1102 98-99 2.495629633714465 1102 100-101 2.8979076200939566 1103 1 0.07606676782527089 1103 2 0.035918348961047286 1103 3 0.19279822810987923 1103 4 0.14899040030806532 1103 5 0.07192877848525825 1103 6 0.1357371454595011 1103 7 0.09100802520585916 1103 8 0.008319035261379781 1103 9 0.005903300455436522 1103 10-11 0.08637629015166937 1103 12-13 0.07312843595836682 1103 14-15 0.2774131316924979 1103 16-17 0.327349490500616 1103 18-19 0.2831261978140347 1103 20-21 0.214328732503013 1103 22-23 0.10296676347676481 1103 24-25 0.17026663112891782 1103 26-27 0.052744134296276 1103 28-29 0.08919582695423145 1103 30-31 0.11068030832928599 1103 32-33 0.10319050171710842 1103 34-35 0.09654819240567036 1103 36-37 0.2935561214695994 1103 38-39 0.3117137668817591 1103 40-41 0.4442021366331801 1103 42-43 0.2797223711385399 1103 44-45 0.3418493502091309 1103 46-47 0.08966873133746134 1103 48-49 0.12035761127514633 1103 50-51 0.23959158507329192 1103 52-53 0.3477652417044581 1103 54-55 0.3916488707977308 1103 56-57 0.4094004795114401 1103 58-59 0.41110809960894557 1103 60-61 0.4141343751584685 1103 62-63 0.6481963158215578 1103 64-65 0.40052499115597584 1103 66-67 0.5299880779448074 1103 68-69 0.8480930354576728 1103 70-71 0.8881520747778673 1103 72-73 0.7906148379768574 1103 74-75 0.8778024253377872 1103 76-77 0.9449746386295743 1103 78-79 1.0717781828038042 1103 80-81 1.2408938696970218 1103 82-83 1.1516446322268017 1103 84-85 1.2661699144694332 1103 86-87 1.3362209340836344 1103 88-89 1.700465391145098 1103 90-91 1.9073128837579496 1103 92-93 2.105871914819577 1103 94-95 2.2391346706815476 1103 96-97 2.3907926450167025 1103 98-99 2.5544829252271057 1103 100-101 3.495592545974244 1104 1 0.07680973440828254 1104 2 0.20514962620315913 1104 3 0.16695619931579841 1104 4 0.12903596892515878 1104 5 0.1727856666109986 1104 6 0.2310267373231909 1104 7 0.26510490804827924 1104 8 0.21030193051959856 1104 9 0.3213867240454533 1104 10-11 0.21229096456720953 1104 12-13 0.2832806615560344 1104 14-15 0.45571025228423423 1104 16-17 0.41232588067365583 1104 18-19 0.4142614507528819 1104 20-21 0.42402362089291756 1104 22-23 0.2680357768171504 1104 24-25 0.4419662084634908 1104 26-27 0.3222924106137981 1104 28-29 0.2708858282750626 1104 30-31 0.23497779214913095 1104 32-33 0.2952052619865526 1104 34-35 0.46118215753576663 1104 36-37 0.43563774873151573 1104 38-39 0.5984741023724496 1104 40-41 0.5866065415994939 1104 42-43 0.681278473370746 1104 44-45 0.5898466425086895 1104 46-47 0.6269664793231939 1104 48-49 0.5876381884776265 1104 50-51 0.498214950546803 1104 52-53 0.5531335880259434 1104 54-55 0.7679796064997504 1104 56-57 0.8865278404941392 1104 58-59 0.7617742929783802 1104 60-61 0.7670575359034189 1104 62-63 0.6484778176046788 1104 64-65 0.8208979603806554 1104 66-67 0.9797511541566664 1104 68-69 1.013728354437994 1104 70-71 0.9243287687416775 1104 72-73 0.8683811502209515 1104 74-75 0.7887966797295469 1104 76-77 1.0280300474262987 1104 78-79 1.129938432440575 1104 80-81 1.303829743425723 1104 82-83 1.1862189949265805 1104 84-85 1.375887917375259 1104 86-87 1.7861482884621829 1104 88-89 1.7664135816087096 1104 90-91 1.6788977140313648 1104 92-93 1.8326104566238328 1104 94-95 2.011216463047152 1104 96-97 1.9467685728994155 1104 98-99 2.4068232699635956 1104 100-101 2.8363046381681407 1105 1 0.09685802238019292 1105 2 0.12647535052273895 1105 3 -0.020725978032750447 1105 4 0.0717181462737102 1105 5 -0.01222530792806964 1105 6 -0.042597055477507695 1105 7 0.09682791068217256 1105 8 0.15787655738527206 1105 9 0.2215930453799544 1105 10-11 0.11602230785078405 1105 12-13 0.11571042450599123 1105 14-15 0.2320513409585061 1105 16-17 0.22912460762712072 1105 18-19 0.16003405830335993 1105 20-21 0.1688524180834392 1105 22-23 0.21442954327896047 1105 24-25 0.0817137940648891 1105 26-27 0.013223051526878749 1105 28-29 0.05846703986417623 1105 30-31 0.23666677371189593 1105 32-33 0.23311241738603172 1105 34-35 0.2987293480537687 1105 36-37 0.31451395593081344 1105 38-39 0.21660184600721522 1105 40-41 0.42840307364514985 1105 42-43 0.42154954448574955 1105 44-45 0.6988040612971034 1105 46-47 0.6981330728262733 1105 48-49 0.6207011823318851 1105 50-51 0.8008773737250365 1105 52-53 0.8455007961032024 1105 54-55 1.0274791675181874 1105 56-57 0.9496347325046699 1105 58-59 0.9514724931539646 1105 60-61 1.081323339239674 1105 62-63 1.1754477473676204 1105 64-65 1.2439763185896098 1105 66-67 1.0061723569661538 1105 68-69 1.0520909532088396 1105 70-71 1.0670120435441426 1105 72-73 1.0542119228284967 1105 74-75 1.088026267086878 1105 76-77 1.02941503425685 1105 78-79 1.0869785992535697 1105 80-81 1.3699776802123793 1105 82-83 1.3338726384735509 1105 84-85 1.707507759341894 1105 86-87 1.7442167695859752 1105 88-89 1.5500801751117805 1105 90-91 1.449197318947956 1105 92-93 1.6533940387133832 1105 94-95 1.7748764718267829 1105 96-97 2.0688197142514753 1105 98-99 1.8872106711927472 1105 100-101 2.5317820306176593 1106 1 -0.0722775755209426 1106 2 -0.11538969596534088 1106 3 0.11053058092885948 1106 4 -0.009960947899173789 1106 5 -0.24124307465917383 1106 6 0.036224632822225544 1106 7 0.04911181015745569 1106 8 -0.0676320498134757 1106 9 -0.18900439547432057 1106 10-11 -0.3246662680490573 1106 12-13 -0.303846788050393 1106 14-15 -0.40506363648787413 1106 16-17 -0.3035394814827015 1106 18-19 -0.0257129729034844 1106 20-21 -0.017571221795051883 1106 22-23 0.08364522955346843 1106 24-25 -0.22916901915429122 1106 26-27 -0.08364153468318847 1106 28-29 0.13608740256324126 1106 30-31 0.05898933653984528 1106 32-33 0.1464383862712353 1106 34-35 -0.1848635820760265 1106 36-37 -0.4748333698539611 1106 38-39 -0.43698095800640857 1106 40-41 -0.2640207503445424 1106 42-43 -0.12173039659865736 1106 44-45 -0.12531726798577836 1106 46-47 0.025917294496160537 1106 48-49 -0.1006216566628666 1106 50-51 -0.14608847364677757 1106 52-53 -0.08920416569372236 1106 54-55 -0.22730220042804916 1106 56-57 -0.44882517728083116 1106 58-59 -0.671649112295718 1106 60-61 -0.3146225320665934 1106 62-63 -0.4601041259638663 1106 64-65 0.04799822782278795 1106 66-67 0.06292463534229142 1106 68-69 -0.06718456920669524 1106 70-71 -0.3807574492294954 1106 72-73 -0.23308992748909318 1106 74-75 -0.37746853177162976 1106 76-77 0.005100388210639295 1106 78-79 0.10995107462869669 1106 80-81 -0.19181556290646284 1106 82-83 0.02810259336630594 1106 84-85 0.21438615389220672 1106 86-87 0.23399123336209726 1106 88-89 0.2775514077362864 1106 90-91 0.608036497811991 1106 92-93 1.001891691295544 1106 94-95 0.4723329755550374 1106 96-97 0.6827504884390834 1106 98-99 0.7123547382092248 1106 100-101 0.9914101259872616 1107 1 0.013447746877584876 1107 2 0.06928622170665477 1107 3 0.0021630763231712535 1107 4 -0.01787976884170206 1107 5 0.09498243592896927 1107 6 0.037551864850115635 1107 7 0.021351365633769603 1107 8 0.05692719029666904 1107 9 0.05339125833602765 1107 10-11 0.011908383800154354 1107 12-13 0.24958049152014183 1107 14-15 0.2746574466919398 1107 16-17 0.13516146540820984 1107 18-19 0.4969886376034367 1107 20-21 0.41377423491143617 1107 22-23 0.5825903772327905 1107 24-25 0.4531248141691364 1107 26-27 0.21937346105778488 1107 28-29 0.22969935557527066 1107 30-31 0.1441798042405651 1107 32-33 0.20448993041655683 1107 34-35 0.1631411127596536 1107 36-37 0.3109919901824938 1107 38-39 0.3151163657391578 1107 40-41 0.4223624928558749 1107 42-43 0.44289168893847375 1107 44-45 0.42005871505733694 1107 46-47 0.392228381835956 1107 48-49 0.3719818971494604 1107 50-51 0.11995592919785736 1107 52-53 0.19177778791259215 1107 54-55 0.3722092494243654 1107 56-57 0.37290725670423086 1107 58-59 0.28595127200728854 1107 60-61 0.5958821627690867 1107 62-63 0.5389994971814716 1107 64-65 0.3962864451718886 1107 66-67 0.6130226920368855 1107 68-69 0.5743154506027324 1107 70-71 0.6719022148025182 1107 72-73 0.6549211558888111 1107 74-75 0.47368710104071354 1107 76-77 0.795057253169734 1107 78-79 0.9679261048380852 1107 80-81 0.8659400778296522 1107 82-83 1.227995870044662 1107 84-85 1.352366285030655 1107 86-87 1.3372417137408519 1107 88-89 1.1724982689315908 1107 90-91 1.2019765445622532 1107 92-93 1.3293750513716098 1107 94-95 1.8185622499354963 1107 96-97 2.2517999823080643 1107 98-99 2.3830241484823986 1107 100-101 3.035618590558091 1108 1 -0.006106683502164145 1108 2 0.1396988799344996 1108 3 0.016168139614308075 1108 4 -0.05477597137335266 1108 5 0.1443393979542833 1108 6 0.08690882687542967 1108 7 0.03136908715276121 1108 8 0.2738765573852717 1108 9 0.3012165747917237 1108 10-11 0.4392223078507911 1108 12-13 0.18420454215305426 1108 14-15 0.31840428213497773 1108 16-17 0.3620893135094718 1108 18-19 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-1.7910263239922593 2316 78-79 -1.8194745382437034 2316 80-81 -2.31158706929682 2316 82-83 -2.3763709447446573 2316 84-85 -2.3713608511430877 2316 86-87 -2.5057635983337505 2316 88-89 -2.166663808019173 2316 90-91 -2.458345035447447 2316 92-93 -2.6075577536228316 2316 94-95 -2.6250311846845236 2316 96-97 -2.8588666087325976 2316 98-99 -3.3731514316211886 2316 100-101 -3.1223559756355215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3468.0 3 557.0 4 516.0 5 648.0 6 739.0 7 839.0 8 1047.0 9 1301.0 10 1740.0 11 2384.0 12 3543.0 13 4761.0 14 5417.0 15 7083.0 16 9428.0 17 13077.0 18 11216.0 19 7113.0 20 6581.0 21 6908.0 22 7667.0 23 8295.0 24 9439.0 25 10660.0 26 12429.0 27 14663.0 28 17215.0 29 20993.0 30 25792.0 31 32099.0 32 41580.0 33 54814.0 34 75071.0 35 106905.0 36 155550.0 37 206565.0 38 140799.0 39 12767.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75736954463999 19.770206365545594 15.21622113496717 22.256202954847247 2 14.609925255484569 20.675337774757796 41.546920807002074 23.167816162755557 3 18.633786684377196 25.41162542230949 30.927781467471338 25.026806425841976 4 11.900873058573174 16.95231238769152 38.892925925248626 32.25388862848668 5 12.625026949611925 38.06141816557842 35.08117608106443 14.232378803745227 6 30.0785199050797 37.19590730745887 18.23579826646364 14.489774520997797 7 26.763719743850668 34.03721821361039 21.684976329847093 17.514085712691855 8 25.436666836597777 34.91459038106569 20.680679891154316 18.96806289118221 9 25.89522750532885 16.943337206528515 21.59328757319954 35.568147714943095 10-11 23.84857749824571 27.362207091322627 28.092347545124564 20.696867865307098 12-13 24.78200477689698 25.05317015314736 28.811874330864086 21.352950739091582 14-15 22.24213357342383 26.070018699355913 27.607617486591103 24.08023024062916 16-17 20.554642362149742 29.43299826776455 28.36595662005373 21.64640275003198 18-19 20.333241805405216 27.62812988245794 31.49091300791388 20.54771530422296 20-21 21.452745754105 26.61374786130381 31.51887582722701 20.414630557364184 22-23 21.002784443664307 26.607499242573613 31.493885284768275 20.8958310289938 24-25 20.68608653290802 26.81501593956383 31.585968567151273 20.912928960376874 26-27 20.398836146780166 26.9641706343481 31.6161689029962 21.020824315875537 28-29 20.43886755337952 27.199303583323513 31.612026654290197 20.749802209006766 30-31 20.565641349736097 26.89910646693235 31.59272822415954 20.942523959172014 32-33 20.40121120363361 27.02906907958335 31.455945257289393 21.113774459493644 34-35 20.87795972214407 26.986875284663814 31.306070054592926 20.829094938599187 36-37 20.857354731854826 26.707851703090707 31.336263030226085 21.098530534828384 38-39 20.519731315393777 27.237423501391845 31.265095957757527 20.97774922545685 40-41 20.772128033097385 27.157001482438893 31.24363784638125 20.827232638082474 42-43 20.610136758770494 27.067214682753217 30.92741960691531 21.395228951560977 44-45 20.69501289011162 27.3325187148263 30.837590364226802 21.13487803083528 46-47 20.87254396528176 27.544426960499536 30.19672323513683 21.386305839081878 48-49 20.86224227237968 27.652544451551776 30.075075869432432 21.41013740663611 50-51 21.00337420598431 27.99667581075206 29.77510743979716 21.22484254346647 52-53 21.06044617253762 28.0535961129447 29.40864967923713 21.477308035280547 54-55 20.847598889823495 27.93355869666317 29.639606234571197 21.57923617894214 56-57 20.98884508125781 28.32796422732955 29.329406673718626 21.353784017694007 58-59 21.10771059106551 27.857662756228862 29.64841635441816 21.386210298287462 60-61 21.029002635903876 27.790802897667117 29.93996370703674 21.24023075939227 62-63 20.777807106491597 27.81193851739986 30.177756045681637 21.232498330426903 64-65 20.83133588525857 27.773752161932013 29.92969784160209 21.46521411120733 66-67 20.82085393465884 27.961770607295744 29.52473865987486 21.692636798170557 68-69 20.815937755284065 27.917660957278876 29.73707454258625 21.529326744850806 70-71 20.85031679760583 27.86506253969887 29.667950333674888 21.61667032902041 72-73 20.836741534077436 27.845396801124096 29.51638893737711 21.801472727421363 74-75 20.710613762160552 28.205600465503228 29.18782189692566 21.895963875410562 76-77 20.763024649175915 27.91329099223308 29.446188218966356 21.877496139624643 78-79 20.942746500309443 27.79142127998438 29.316084655219587 21.949747564486586 80-81 20.796728912415315 28.004335547110365 29.16319035869728 22.035745181777045 82-83 20.89246947327424 27.928846704027194 29.370472680413613 21.808211142284957 84-85 20.837666580602725 27.920344217296545 29.177887433174238 22.064101768926495 86-87 20.611910379569842 27.99135857259698 29.279783281540222 22.116947766292952 88-89 20.82901758708671 27.6692469780159 29.256047093807737 22.245688341089657 90-91 20.69662415647188 27.47365191776725 29.441283134053776 22.388440791707097 92-93 20.640428054741616 27.365278444262586 29.621480439325413 22.37281306167039 94-95 20.44924532747354 27.404349644902414 29.593212515206446 22.553192512417596 96-97 20.37381748447156 27.526879341941196 29.589420125593215 22.509883047994034 98-99 20.279416297488822 27.674561855261125 29.268938907618264 22.77708293963179 100-101 20.612531996361405 27.069883322659866 29.872293410720772 22.445291270257954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1270.0 1 733.0 2 201.0 3 208.5 4 211.0 5 241.0 6 354.0 7 649.5 8 1068.5 9 1394.5 10 1457.0 11 1467.5 12 1612.0 13 1797.5 14 1891.5 15 1940.5 16 2066.5 17 2150.5 18 2288.5 19 2497.0 20 2738.5 21 3075.5 22 3591.0 23 4576.5 24 5600.5 25 6630.5 26 8311.5 27 10277.0 28 11959.0 29 13644.0 30 15499.5 31 17658.5 32 19979.5 33 22355.5 34 25112.0 35 27519.0 36 30518.0 37 33280.0 38 34384.5 39 34800.5 40 35096.0 41 36390.5 42 37104.0 43 37523.5 44 38645.0 45 39601.5 46 40119.0 47 40714.0 48 42206.0 49 41047.5 50 37042.0 51 33575.5 52 30835.5 53 29221.5 54 27717.0 55 24779.0 56 21462.0 57 18509.0 58 16089.5 59 13893.5 60 11504.0 61 9025.0 62 6767.0 63 4792.5 64 3304.0 65 2371.0 66 1654.0 67 1155.5 68 809.0 69 600.0 70 463.5 71 343.0 72 252.5 73 189.5 74 147.0 75 114.0 76 78.5 77 46.5 78 25.5 79 20.0 80 14.0 81 9.5 82 9.5 83 7.0 84 4.5 85 4.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 1.0 92 2.0 93 2.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3046070252575192 2 0.2557431816218188 3 0.2630391582746935 4 0.2571831770138336 5 0.2586231724058483 6 0.19785536686282604 7 0.18854339666113068 8 0.19516737546439852 9 0.19641537147081128 10-11 0.1992473624084403 12-13 0.19996736010444768 14-15 0.19795136655562703 16-17 0.18940739389633954 18-19 0.18379141186748202 20-21 0.18249541601466876 22-23 0.18863939635393165 24-25 0.18830339742912822 26-27 0.19391937945798574 28-29 0.1979993664020275 30-31 0.20054335826125355 32-33 0.19612737239240835 34-35 0.19838336517323144 36-37 0.18931139420353854 38-39 0.1788954275346319 40-41 0.17563143797939845 42-43 0.18427141033148692 44-45 0.18427141033148692 46-47 0.1906553899027523 48-49 0.19727936870602014 50-51 0.19372738007238377 52-53 0.2032313496596811 54-55 0.1934873808403813 56-57 0.16991945625774 58-59 0.1567674983440053 60-61 0.17270344734896848 62-63 0.1664154674705041 64-65 0.17294344658097094 66-67 0.17179145026735915 68-69 0.1703514548753444 70-71 0.1738074438161798 72-73 0.1822554167826663 74-75 0.18575940556990217 76-77 0.1861914041875066 78-79 0.1823514164754673 80-81 0.1817754183186614 82-83 0.1730394462737719 84-85 0.16756746378411588 86-87 0.17236744842416504 88-89 0.17980742461624122 90-91 0.17673543444660977 92-93 0.17308744612017243 94-95 0.17423944243378423 96-97 0.18091142108345254 98-99 0.17851142876342796 100-101 0.16396747530407904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1041670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.06816299907042 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80047509770286 43.82862031281755 2 13.133398792509329 14.98997886045434 3 4.348521741765379 7.444864426922047 4 1.9822564920677328 4.524949463811477 5 0.9795479139196829 2.795050000848393 6 0.6297925646900684 2.1564662842401257 7 0.39392822243935877 1.5736532005672377 8 0.2731937432404961 1.2472532055659857 9 0.20798870217927956 1.0682579842139015 >10 1.1308541322062668 11.933268998825246 >50 0.0814500319347658 3.1568847512022047 >100 0.036899909434697585 3.4552668763249916 >500 8.463279549904417E-4 0.33219626923675133 >1k 6.770623639923534E-4 0.9370852014730227 >5k 1.6926559099808834E-4 0.5562041634967182 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5758 0.5527662311480603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4387 0.4211506523179126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2859 0.2744631217180105 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1355 0.13007958374533202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1100 0.10559966208108133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.599969280098303E-5 0.0 0.0 0.0 0.0 8 9.599969280098303E-5 0.0 0.0 0.0 0.0 9 9.599969280098303E-5 0.0 0.0 0.0 0.0 10-11 9.599969280098303E-5 0.0 0.0 0.0 0.0 12-13 9.599969280098303E-5 0.0 0.0 0.0 0.0 14-15 1.9199938560196606E-4 0.0 0.0 4.7999846400491516E-5 0.0 16-17 1.9199938560196606E-4 0.0 0.0 1.9199938560196606E-4 0.0 18-19 1.9199938560196606E-4 0.0 0.0 1.9199938560196606E-4 0.0 20-21 1.9199938560196606E-4 0.0 0.0 1.9199938560196606E-4 0.0 22-23 1.9199938560196606E-4 0.0 0.0 4.3199861760442366E-4 0.0 24-25 2.879990784029491E-4 0.0 0.0 8.159973888083558E-4 0.0 26-27 3.3599892480344065E-4 0.0 0.0 0.001295995852813271 0.0 28-29 6.719978496068813E-4 0.0 0.0 0.002975990476830474 0.0 30-31 6.719978496068813E-4 0.0 0.0 0.006095980492862423 0.0 32-33 6.719978496068813E-4 0.0 0.0 0.011567962982518457 0.0 34-35 6.719978496068813E-4 0.0 0.0 0.01713594516497547 0.0 36-37 6.719978496068813E-4 0.0 0.0 0.022463928115430028 0.0 38-39 6.719978496068813E-4 0.0 0.0 0.027743911219484097 0.0 40-41 6.719978496068813E-4 0.0 0.0 0.036335883725172075 0.0 42-43 7.199976960073728E-4 0.0 0.0 0.04723184885808365 0.0 44-45 0.0010559966208108134 0.0 0.0 0.060575806157420295 0.0 46-47 0.0011519963136117964 0.0 0.0 0.07622375608398053 0.0 48-49 0.0011519963136117964 0.0 0.0 0.09019171138652357 4.7999846400491516E-5 50-51 0.0011519963136117964 0.0 0.0 0.10891165148271525 9.599969280098303E-5 52-53 0.0011519963136117964 0.0 0.0 0.15926349035683085 9.599969280098303E-5 54-55 0.0011519963136117964 0.0 0.0 0.2331832538135878 9.599969280098303E-5 56-57 0.0011519963136117964 0.0 0.0 0.2938070598174086 9.599969280098303E-5 58-59 0.0011519963136117964 0.0 0.0 0.3864947632167577 9.599969280098303E-5 60-61 0.0011519963136117964 0.0 0.0 0.5032783895091536 9.599969280098303E-5 62-63 0.0011519963136117964 0.0 0.0 0.5765741549627041 1.9199938560196606E-4 64-65 0.0011519963136117964 0.0 0.0 0.6393579540545471 1.9199938560196606E-4 66-67 0.0012479960064127795 0.0 0.0 0.8134533969491298 1.9199938560196606E-4 68-69 0.0012479960064127795 0.0 0.0 0.9779968704100147 1.9199938560196606E-4 70-71 0.0012479960064127795 0.0 0.0 1.0621406011500762 1.9199938560196606E-4 72-73 0.0012479960064127795 0.0 0.0 1.2336440523390326 1.9199938560196606E-4 74-75 0.0012479960064127795 0.0 0.0 1.4974992080025344 1.9199938560196606E-4 76-77 0.0012479960064127795 0.0 0.0 1.7732103257269576 1.9199938560196606E-4 78-79 0.0012479960064127795 0.0 0.0 2.020697533767892 1.9199938560196606E-4 80-81 0.0012479960064127795 0.0 0.0 2.288200677757831 1.9199938560196606E-4 82-83 0.0012479960064127795 0.0 0.0 2.5318958979331265 1.9199938560196606E-4 84-85 0.001295995852813271 0.0 0.0 2.8389989152034714 1.9199938560196606E-4 86-87 0.0013439956992137626 0.0 0.0 3.39258114374034 1.9199938560196606E-4 88-89 0.0013439956992137626 0.0 0.0 3.9905152303512628 1.9199938560196606E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 35.718468 1 GTATCAA 5790 0.0 34.896725 1 ATCAACG 7190 0.0 27.9588 3 TCAACGC 7290 0.0 27.450182 4 CAACGCA 7320 0.0 27.33768 5 TATCAAC 7365 0.0 27.229942 2 ATACCGA 70 0.008388824 27.144436 6 AACGCAG 7465 0.0 26.917173 6 GTCGGGA 120 1.7049714E-4 23.761675 2 CGCAGAG 8475 0.0 23.204891 8 ACGCAGA 8535 0.0 23.153076 7 TACATGG 4685 0.0 22.924818 2 GTACATG 4940 0.0 22.519732 1 GTATAGG 255 4.8567017E-10 22.372545 1 TGTATCG 170 3.1469135E-6 22.354244 9 GAGTACT 4720 0.0 22.190374 12-13 ACATGGG 4790 0.0 21.926222 3 GCAGAGT 8855 0.0 21.833569 9 CCCTATA 200 5.3596705E-7 21.385508 2 AGTACAT 3755 0.0 21.13555 2 >>END_MODULE