FastQCFastQC Report
Thu 26 May 2016
SRR937668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041670
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78050.7492776023116726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64580.6199660161087485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.4076146956329739No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.1515835149327522No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14640.14054355026063917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54600.035.776321
GTATCAA81700.032.053541
ATCAACG101150.025.9245433
TCAACGC101550.025.869214
CAACGCA102500.025.6757935
TATCAAC102900.025.5298162
AACGCAG104200.025.2076746
GCGGTAT950.001222321125.014561
TCCGACC950.001226777324.9989538
GAGTACT65650.024.12884512-13
GGACCGA1801.9514482E-723.7490046
GGACCGT1201.711153E-423.7490026
AGTACTT67900.023.1893812-13
GTACATG52650.022.9288271
GTACTTT68450.022.89951714-15
TACATGG51950.022.8608532
ACGCAGA116300.022.5033557
CGCAGAG117050.022.3591658
AGGACCG2354.642061E-922.2363135
CCGCCGT753.0345978E-522.16680190-91
Produced by FastQC (version 0.11.5)