##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937668_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1041670 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40156479499266 33.0 31.0 34.0 31.0 34.0 2 32.61133564372594 34.0 31.0 34.0 31.0 34.0 3 32.71360603646068 34.0 31.0 34.0 31.0 34.0 4 36.29126978793668 37.0 35.0 37.0 35.0 37.0 5 36.17549607841255 37.0 35.0 37.0 35.0 37.0 6 36.274219282498294 37.0 37.0 37.0 35.0 37.0 7 36.239805312623 37.0 37.0 37.0 35.0 37.0 8 36.23870419614657 37.0 37.0 37.0 35.0 37.0 9 37.92676183436213 39.0 38.0 39.0 35.0 39.0 10-11 37.894342738103234 39.0 38.0 39.0 35.0 39.0 12-13 37.82928374629201 39.0 38.0 39.0 35.0 39.0 14-15 39.08922499448002 40.0 38.0 41.0 36.0 41.0 16-17 39.064215634509964 40.0 38.0 41.0 36.0 41.0 18-19 39.088314437393805 40.0 38.5 41.0 36.0 41.0 20-21 39.03205717741703 40.0 38.0 41.0 36.0 41.0 22-23 38.89372786007085 40.0 38.0 41.0 35.0 41.0 24-25 38.84377297992646 40.0 38.0 41.0 35.0 41.0 26-27 38.773478644868334 40.0 38.0 41.0 35.0 41.0 28-29 38.65712029721505 40.0 38.0 41.0 35.0 41.0 30-31 38.67655975500878 40.0 38.0 41.0 35.0 41.0 32-33 38.55203087350121 40.0 38.0 41.0 35.0 41.0 34-35 38.40018719940096 40.0 38.0 41.0 35.0 41.0 36-37 38.23233413653077 40.0 38.0 41.0 34.0 41.0 38-39 38.09413297877447 40.0 38.0 41.0 34.0 41.0 40-41 37.903921587450924 40.0 37.5 41.0 34.0 41.0 42-43 37.6543219061699 40.0 37.0 41.0 33.0 41.0 44-45 37.2760154367506 40.0 37.0 41.0 33.0 41.0 46-47 36.79577937350601 40.0 37.0 41.0 31.5 41.0 48-49 36.3460035327887 40.0 36.5 41.0 30.5 41.0 50-51 36.05227903270709 40.0 36.0 41.0 29.5 41.0 52-53 35.82530791901466 39.0 36.0 41.0 29.0 41.0 54-55 35.63073862163641 39.0 35.5 41.0 28.0 41.0 56-57 35.35912621079613 39.0 35.0 40.0 27.5 41.0 58-59 35.126965833709335 39.0 35.0 40.0 27.0 41.0 60-61 34.7872090009312 38.0 35.0 40.0 26.0 41.0 62-63 34.43730692061786 38.0 34.0 40.0 26.0 41.0 64-65 34.05169007459176 37.5 34.0 40.0 25.0 41.0 66-67 33.64516497547208 37.0 34.0 39.5 23.5 41.0 68-69 33.19594593297302 36.0 33.0 39.0 22.0 40.0 70-71 32.77838326917354 36.0 33.0 39.0 21.5 40.0 72-73 32.34419009859168 35.0 32.0 38.0 20.0 40.0 74-75 31.856929257826373 35.0 32.0 37.0 18.5 39.0 76-77 30.645186095404497 34.0 30.5 36.0 18.0 38.5 78-79 31.15883437173001 35.0 31.0 36.0 17.5 39.0 80-81 30.952065913389077 35.0 31.0 36.0 14.0 38.0 82-83 30.598827363752434 34.0 31.0 35.5 9.0 37.0 84-85 30.209796768650342 34.0 31.0 35.0 7.0 37.0 86-87 29.817940422590645 34.0 30.5 35.0 2.0 36.0 88-89 29.413184597809288 34.0 30.0 35.0 2.0 36.0 90-91 28.94460673725844 34.0 29.0 35.0 2.0 35.5 92-93 28.531786938281797 34.0 29.0 35.0 2.0 35.0 94-95 28.116243628020392 34.0 29.0 35.0 2.0 35.0 96-97 27.62179145026736 33.5 28.0 35.0 2.0 35.0 98-99 26.87920934653009 33.0 26.5 35.0 2.0 35.0 100-101 24.495808173413845 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16538975294627534 1101 2 0.14771009458316087 1101 3 0.1772234888929205 1101 4 0.14533261339598624 1101 5 0.0100809701556841 1101 6 0.07371985513201906 1101 7 0.1735124505190626 1101 8 0.14804013429197482 1101 9 0.20192971239041668 1101 10-11 0.21595871809326894 1101 12-13 0.16501747593403593 1101 14-15 0.1828860916045798 1101 16-17 0.09796127828852974 1101 18-19 0.19037786387796274 1101 20-21 0.2942352551548524 1101 22-23 0.27581592706333424 1101 24-25 0.21961653321972818 1101 26-27 0.1777459881743937 1101 28-29 0.190238387056489 1101 30-31 0.18943348556130246 1101 32-33 0.23289079886799158 1101 34-35 0.07495588403744335 1101 36-37 0.12627397547387176 1101 38-39 0.24340960016520796 1101 40-41 0.13650205126963044 1101 42-43 0.16493730209717938 1101 44-45 0.06340436226559376 1101 46-47 -0.20354592216825296 1101 48-49 -0.007321267340081761 1101 50-51 -0.0633340312599131 1101 52-53 0.125609265877074 1101 54-55 0.14315694533890166 1101 56-57 0.05631963521548755 1101 58-59 0.10333224650710804 1101 60-61 0.0547621952305235 1101 62-63 0.041473618431545844 1101 64-65 0.11318597428471122 1101 66-67 0.0017805255742260329 1101 68-69 -0.06741463021163696 1101 70-71 0.1281046808330828 1101 72-73 0.014017883897231798 1101 74-75 -0.07471507103953812 1101 76-77 -0.09969663366335269 1101 78-79 -0.04786802035165039 1101 80-81 -0.030074012239559522 1101 82-83 -0.16147040616099062 1101 84-85 0.06975579427192002 1101 86-87 0.22006651183797388 1101 88-89 0.41169259794314783 1101 90-91 0.24932085955254735 1101 92-93 0.3287450198007491 1101 94-95 0.24594421843579184 1101 96-97 0.5184824492386291 1101 98-99 0.6599842520032091 1101 100-101 1.4723022236670147 1102 1 0.10577776331324884 1102 2 0.1465475659425124 1102 3 -0.01265618907976318 1102 4 0.06351426084367517 1102 5 -0.10532863807780046 1102 6 0.010240282331558603 1102 7 -0.08037516545208945 1102 8 0.034986884595596734 1102 9 0.10105891145388313 1102 10-11 -0.002499945468322551 1102 12-13 0.06422868428771267 1102 14-15 -0.06642308967978039 1102 16-17 -0.01048505257826804 1102 18-19 0.14429678110492006 1102 20-21 0.08854218274878889 1102 22-23 0.10013355968676052 1102 24-25 0.06927347145609275 1102 26-27 0.01710481325045521 1102 28-29 -0.15704873310277634 1102 30-31 0.021006436268081075 1102 32-33 -0.07463245796507323 1102 34-35 -0.10432111955594081 1102 36-37 -0.0184886984046031 1102 38-39 -0.029457845276695593 1102 40-41 -0.14761882165979046 1102 42-43 -0.04081962487072843 1102 44-45 -0.23704387171755315 1102 46-47 -0.041453308012570744 1102 48-49 -0.18073930187162546 1102 50-51 -0.08348327564760183 1102 52-53 -0.03870198129015989 1102 54-55 -0.11001668898730799 1102 56-57 -0.015709562595930038 1102 58-59 -0.03885871619431214 1102 60-61 0.11393972343294934 1102 62-63 0.022482520508695814 1102 64-65 -0.2343443566321639 1102 66-67 -0.1568748976813552 1102 68-69 0.12640211971636717 1102 70-71 0.14406112200550325 1102 72-73 0.09618023725084868 1102 74-75 0.009418635404760067 1102 76-77 0.051365730958711 1102 78-79 0.2906801021990262 1102 80-81 -0.007545559398032253 1102 82-83 -0.15488180482630654 1102 84-85 -0.10012707642885132 1102 86-87 0.09066931142229251 1102 88-89 0.21510349578836951 1102 90-91 0.3472637410209529 1102 92-93 0.6000405161760618 1102 94-95 0.203871261468759 1102 96-97 0.6122319014666502 1102 98-99 0.9589178182178841 1102 100-101 1.6125262154535633 1103 1 0.11717016837653915 1103 2 0.1589033405588438 1103 3 0.13878699825454532 1103 4 0.07306954257326481 1103 5 -0.07921684240175608 1103 6 0.02881688514698766 1103 7 0.050026985326447004 1103 8 -0.04314313295415673 1103 9 0.12320147521177205 1103 10-11 0.13209734365457138 1103 12-13 0.18485151390572696 1103 14-15 0.18488380700070906 1103 16-17 0.12856315323929124 1103 18-19 0.18657675571378718 1103 20-21 0.06389597835014627 1103 22-23 0.12185791650689026 1103 24-25 0.15250038605972094 1103 26-27 0.07198327185917464 1103 28-29 0.005895145019771064 1103 30-31 0.09941090212073789 1103 32-33 0.13431605030571347 1103 34-35 0.30890654184579347 1103 36-37 0.05029514088443676 1103 38-39 0.011003675951037906 1103 40-41 0.143492413917663 1103 42-43 0.18475821016156857 1103 44-45 0.17205959715963104 1103 46-47 0.21761095396420416 1103 48-49 0.19190735060588082 1103 50-51 0.028605518646863004 1103 52-53 -0.08390006948701512 1103 54-55 0.017689520638924705 1103 56-57 0.2923297189842913 1103 58-59 0.0875947396139054 1103 60-61 0.323896409142975 1103 62-63 0.4038984497122371 1103 64-65 0.5423107346639071 1103 66-67 0.2560372415218026 1103 68-69 0.1531113538749196 1103 70-71 0.5225290656969221 1103 72-73 0.3915519560066869 1103 74-75 0.4565583986697668 1103 76-77 0.40377228784605634 1103 78-79 0.3303481504421839 1103 80-81 0.23860656337557629 1103 82-83 0.3563239007112031 1103 84-85 0.26150430402744007 1103 86-87 0.4476294697434895 1103 88-89 0.5568233718198847 1103 90-91 0.5067891508264104 1103 92-93 0.6018720385239966 1103 94-95 0.5732301352972193 1103 96-97 0.9483825306440536 1103 98-99 1.1053369213133628 1103 100-101 1.6132412746373141 1104 1 0.100379123600419 1104 2 0.14291304865552945 1104 3 0.08369354956876407 1104 4 0.07808539252703639 1104 5 -0.15742810907723026 1104 6 -0.08882609464432534 1104 7 0.01212908544532354 1104 8 0.06411812711716891 1104 9 0.11887410762492578 1104 10-11 0.18998187013719559 1104 12-13 0.10692422556864756 1104 14-15 0.017021239308199654 1104 16-17 0.02760967945776116 1104 18-19 0.038627343482900756 1104 20-21 0.09193306063782103 1104 22-23 0.02788089893985557 1104 24-25 0.0728139179577525 1104 26-27 -0.07580681531557332 1104 28-29 -0.02291875010636346 1104 30-31 0.10887051775935674 1104 32-33 0.18473211159220426 1104 34-35 0.13556253180749422 1104 36-37 -0.01902281579235421 1104 38-39 -0.12514082279417948 1104 40-41 -0.08422849481301142 1104 42-43 0.05191330856336407 1104 44-45 -0.15600720379136845 1104 46-47 0.04000726884995487 1104 48-49 0.10633245957431114 1104 50-51 -6.624116133409075E-4 1104 52-53 -0.04032755594852233 1104 54-55 -0.05995213832290602 1104 56-57 0.0786855504464512 1104 58-59 -0.0033542763681282395 1104 60-61 0.08756369219672422 1104 62-63 0.25419893841914387 1104 64-65 0.24535524661741448 1104 66-67 0.28961850555561597 1104 68-69 0.26192623962317185 1104 70-71 0.26913866152310106 1104 72-73 0.18468116615066066 1104 74-75 0.1452611790158187 1104 76-77 -0.24949428856392686 1104 78-79 -0.0683987125878005 1104 80-81 0.09570519631706276 1104 82-83 0.12705266837729567 1104 84-85 0.138852078429764 1104 86-87 0.3168945932410452 1104 88-89 0.26984757602012266 1104 90-91 0.21193130509929503 1104 92-93 0.4695769321972243 1104 94-95 0.6304220518207941 1104 96-97 0.9652775907417954 1104 98-99 1.2065900582833784 1104 100-101 1.9958930049239356 1105 1 0.09834663896477736 1105 2 0.12702411099091648 1105 3 0.05223832568817244 1105 4 -0.04061083223152906 1105 5 -0.01442767009567092 1105 6 0.022372325022047335 1105 7 0.10066727684128551 1105 8 0.0874763360723918 1105 9 0.21425821649235388 1105 10-11 0.0963086919106857 1105 12-13 0.03100455919463485 1105 14-15 0.12811649830732108 1105 16-17 0.059979082442836784 1105 18-19 0.11403120652065013 1105 20-21 0.08143371911017283 1105 22-23 0.046256667157585696 1105 24-25 -0.08039393981221821 1105 26-27 0.004200866861779673 1105 28-29 0.019205920657462627 1105 30-31 -0.00439440761377341 1105 32-33 -0.06067614126117604 1105 34-35 -0.27849585274035604 1105 36-37 -0.1494914286106095 1105 38-39 -0.30380631884334264 1105 40-41 -0.125690303417052 1105 42-43 -0.10835995745946292 1105 44-45 0.003996088570353606 1105 46-47 0.04622105287102585 1105 48-49 -0.265276407853257 1105 50-51 -0.44869796911992665 1105 52-53 -0.5848995489248097 1105 54-55 -0.37342448248445237 1105 56-57 -0.36114763655969284 1105 58-59 -0.26123399541992853 1105 60-61 -0.061694428962184134 1105 62-63 -0.19869395007953727 1105 64-65 -0.1589906708540525 1105 66-67 -0.16964729778063514 1105 68-69 -0.07796620989395109 1105 70-71 0.006126150548560361 1105 72-73 -0.0538098347273106 1105 74-75 0.11422627998157964 1105 76-77 -0.1646556142882467 1105 78-79 0.07653763508560019 1105 80-81 0.07231296629072403 1105 82-83 -0.17195303838301612 1105 84-85 -0.2393129786378374 1105 86-87 -0.011100358470979899 1105 88-89 0.12121171122644725 1105 90-91 0.1575491716489026 1105 92-93 0.09230410515595722 1105 94-95 0.28223065585942564 1105 96-97 0.2069874239288012 1105 98-99 0.8838793471332487 1105 100-101 1.3797066572505372 1106 1 -0.0901850741899679 1106 2 -0.016654430848355162 1106 3 -0.14942051625236275 1106 4 -0.16714677994394123 1106 5 -0.09612517538886323 1106 6 -0.14026782963136242 1106 7 -0.1415070764681161 1106 8 -0.18870519757393112 1106 9 -0.09121540957779217 1106 10-11 -0.012804812646066921 1106 12-13 -0.13766707847451443 1106 14-15 0.09753562746133326 1106 16-17 0.060135576965528514 1106 18-19 0.14096483443191232 1106 20-21 0.08470905741450707 1106 22-23 -0.025759350517084556 1106 24-25 -0.07335444795914015 1106 26-27 -0.06589671207590442 1106 28-29 -0.025248649903154785 1106 30-31 -0.31184354689389693 1106 32-33 -0.08658150810274634 1106 34-35 -0.41972295390946357 1106 36-37 -0.30702894126825697 1106 38-39 -0.19888594693870942 1106 40-41 -0.5093826535965604 1106 42-43 -0.16618560415006556 1106 44-45 0.002610651766524086 1106 46-47 -0.01567529252159261 1106 48-49 -0.11528101063333906 1106 50-51 -0.23791365539443632 1106 52-53 -0.30517847739761805 1106 54-55 -0.2020731062532093 1106 56-57 -0.2627411190231044 1106 58-59 -0.4297463768983434 1106 60-61 -0.16845959512254183 1106 62-63 0.019822113622943505 1106 64-65 -0.10439249355496116 1106 66-67 -0.13085322616138484 1106 68-69 -0.5007407657256735 1106 70-71 -0.2672163883449272 1106 72-73 -0.027309788699504622 1106 74-75 -0.053341611024588786 1106 76-77 -0.3959867382871387 1106 78-79 -0.1106887296386958 1106 80-81 -0.1431569775553605 1106 82-83 -0.37176340384375806 1106 84-85 -0.16731076930340194 1106 86-87 -0.09852464273867767 1106 88-89 -0.050045768291184345 1106 90-91 0.23241062796851963 1106 92-93 0.30847735379813557 1106 94-95 0.05453388701104345 1106 96-97 0.29556424432832173 1106 98-99 0.20655356235924316 1106 100-101 1.1286075133676334 1107 1 0.1567271304018547 1107 2 0.2411678191070692 1107 3 0.12236490796664867 1107 4 0.12680540008418717 1107 5 -0.14931597984995193 1107 6 0.11899555659316263 1107 7 0.07152356872512655 1107 8 0.003235829743275076 1107 9 0.23755680174924265 1107 10-11 0.10446207984814038 1107 12-13 0.14840589947758076 1107 14-15 0.20377733226116135 1107 16-17 0.1522259178858718 1107 18-19 0.24308370093613973 1107 20-21 0.19033543169393852 1107 22-23 0.31838250483443886 1107 24-25 0.4516574377008098 1107 26-27 0.32723139552894054 1107 28-29 0.18282468461874402 1107 30-31 0.14911825061407313 1107 32-33 0.22193927199273134 1107 34-35 0.29478225597149077 1107 36-37 0.2366273353506685 1107 38-39 0.10218772434355117 1107 40-41 0.18640016568198092 1107 42-43 0.33043564939087133 1107 44-45 0.33916920845121723 1107 46-47 0.19631040506759945 1107 48-49 -0.05420976600664318 1107 50-51 0.023426007052826492 1107 52-53 -0.0028370023872099637 1107 54-55 -0.014077870421907335 1107 56-57 -0.2279458495157627 1107 58-59 -0.09550428581457027 1107 60-61 0.1665241116186067 1107 62-63 -0.25220102379510223 1107 64-65 -0.1691544832144416 1107 66-67 -0.1776554883986634 1107 68-69 -0.42148817564227414 1107 70-71 -0.17012664170758285 1107 72-73 -0.23208980494324294 1107 74-75 -0.00835562619861463 1107 76-77 -0.267885323893605 1107 78-79 -0.13943593155624967 1107 80-81 -0.0678303695246143 1107 82-83 -0.14137969512166393 1107 84-85 -0.15023256166092125 1107 86-87 -0.015448832037620264 1107 88-89 0.3074030738474427 1107 90-91 0.3924114203458444 1107 92-93 0.5639645668089734 1107 94-95 0.693217253029939 1107 96-97 0.6150272601164382 1107 98-99 0.6814389152642981 1107 100-101 1.503243515031624 1108 1 0.036370168376535617 1108 2 0.1319652874615045 1108 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 0.0 5 13.0 6 28.0 7 71.0 8 183.0 9 388.0 10 645.0 11 1249.0 12 2175.0 13 3393.0 14 4368.0 15 6332.0 16 9609.0 17 15193.0 18 13837.0 19 6884.0 20 5057.0 21 4778.0 22 4863.0 23 4994.0 24 5307.0 25 6217.0 26 7462.0 27 9243.0 28 11104.0 29 14143.0 30 18013.0 31 23544.0 32 31015.0 33 42590.0 34 61382.0 35 93716.0 36 153278.0 37 250632.0 38 210618.0 39 19340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.279820589040376 20.426053006910394 15.626329614813889 20.667796789235343 2 15.45193338766921 21.64545551417965 40.42416544986939 22.47844564828175 3 19.258114374034 26.02148473124886 31.132604375665995 23.58779651905114 4 12.670231455259342 17.816774986320045 38.113318037382285 31.399675521038333 5 12.766110658252131 38.66922828029122 34.30824430573838 14.256416755718265 6 30.380350782877496 37.911718682500215 17.309416609866847 14.398513924755441 7 26.76865034031891 34.830800541438265 20.945980972860887 17.454568145381934 8 25.944204978544068 35.541870266015145 19.774112722839288 18.739812032601495 9 26.577130953180948 17.014025555118224 21.107836454923344 35.301007036777484 10-11 24.461153724308083 27.95583054134227 27.06418539460674 20.518830339742912 12-13 25.631581625354855 25.602156617842596 28.173940675728737 20.592321081073816 14-15 22.78379909184291 26.491643226741672 27.424232242456824 23.300325438958595 16-17 20.617086025324717 29.933088214117713 28.158005894381137 21.29181986617643 18-19 20.422206648938722 27.695863373237206 31.862586039724672 20.0193439380994 20-21 21.651013109244342 26.49467993447058 32.239928307395004 19.614378648890074 22-23 21.20011539201846 26.579044252647083 32.005445120871215 20.215395234463237 24-25 20.915133699798975 26.748647833942297 32.23992043484355 20.09629803141518 26-27 20.636971720279654 26.862446269011347 32.369827604827925 20.130754405881067 28-29 20.600927383741023 27.24021734956415 32.23988134096233 19.9189739257325 30-31 20.791041265666024 26.84865095784153 32.196819514911226 20.163488261581218 32-33 20.862125241199227 26.99592961302524 31.88360997244809 20.258335173327445 34-35 21.120748413605074 26.967513703956147 32.02717751303196 19.884560369406817 36-37 21.247228008870373 26.68383461172924 31.888313957395336 20.180623422005052 38-39 20.77222152889111 27.270632733975255 31.830570142175546 20.126575594958098 40-41 21.04289266274348 27.244904816304587 31.755642381944376 19.956560139007557 42-43 20.938549652949963 27.139845876083523 31.321920053918888 20.599684417047623 44-45 21.206524139122752 27.35847245288815 31.09842848502885 20.336574922960246 46-47 21.36228364069235 27.859014851152473 30.229007267176744 20.54969424097843 48-49 21.57708295333455 27.990150431518618 29.909808288613476 20.522958326533356 50-51 21.65887469160099 28.392245144815536 29.54001747194409 20.40886269163939 52-53 21.63185077807751 28.58285253487189 29.10326686954602 20.682029817504585 54-55 21.34985168047462 28.538788675876237 29.243714420113854 20.867645223535288 56-57 21.473163285877487 28.81440379390786 29.166002668791457 20.546430251423196 58-59 21.632138777155912 28.229285666285868 29.397505927981033 20.74106962857719 60-61 21.43769139938752 28.26859754048787 29.746560811005406 20.547150249119202 62-63 21.32911574682961 28.125557998214408 29.997792007065577 20.547534247890407 64-65 21.346059692608986 28.27939750592798 29.698896963529712 20.675645837933317 66-67 21.28399589121315 28.400741117628424 29.388097958086533 20.927165033071894 68-69 21.42285944684977 28.375685197807364 29.4142578743748 20.78719748096806 70-71 21.44321138172358 28.437796999049603 29.260178367429223 20.858813251797596 72-73 21.50610150340391 28.16272339739236 29.221792516724353 21.109382582479373 74-75 21.366555627021995 28.799475841677307 28.63147637927559 21.202492152025112 76-77 21.424251442395384 28.485796845450096 29.082674935440206 21.007276776714313 78-79 21.50081119740417 28.406501099196486 28.76165196271372 21.331035740685632 80-81 21.404907504295988 28.788675876237196 28.50418078662148 21.302235832845334 82-83 21.437492560009524 28.80404313082479 28.672331299415937 21.086133009749748 84-85 21.33543444963537 28.75170939699146 28.535325777826632 21.377530375546538 86-87 21.082540535870283 28.847331688538596 28.510612766039152 21.55951500955197 88-89 21.3769715936909 28.706404139506752 28.465588910115486 21.45103535668686 90-91 21.259263455111547 28.51180835508687 28.578821094227784 21.650107095573798 92-93 21.12964806560405 28.27201834025669 28.80948655153445 21.788847042604807 94-95 20.967148905123505 28.555300623038004 28.822467768103145 21.65508270373535 96-97 20.953420949052965 28.49131682778615 28.843491700826558 21.711770522334326 98-99 20.83556332940854 28.627197616132193 28.395454024000916 22.141785030458347 100-101 20.606320709292785 29.125831877562604 28.372911575069526 21.89493583807509 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 278.0 1 207.5 2 182.5 3 243.5 4 231.0 5 235.0 6 412.0 7 880.5 8 1478.5 9 1922.0 10 2021.5 11 2006.0 12 2146.0 13 2365.5 14 2564.0 15 2647.5 16 2716.5 17 2823.5 18 2925.0 19 3009.0 20 3192.5 21 3527.0 22 3958.0 23 4761.0 24 5713.5 25 6835.5 26 8528.0 27 10376.0 28 11950.0 29 13606.5 30 15753.0 31 17729.5 32 19638.0 33 21939.5 34 24614.0 35 26862.5 36 30015.0 37 32722.0 38 33791.5 39 34701.0 40 34912.0 41 35856.5 42 36522.5 43 37020.0 44 38259.5 45 38799.0 46 38933.0 47 40121.5 48 42244.5 49 40394.0 50 36270.5 51 33289.0 52 30438.0 53 28938.5 54 27514.5 55 25004.5 56 21464.0 57 18599.5 58 16835.0 59 14717.0 60 11832.5 61 9031.5 62 6932.5 63 4893.0 64 3333.0 65 2346.5 66 1621.5 67 1173.5 68 836.0 69 587.0 70 447.0 71 319.0 72 219.0 73 182.5 74 139.0 75 101.0 76 79.0 77 40.0 78 19.5 79 13.0 80 8.5 81 4.0 82 1.0 83 2.0 84 3.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046559851008476774 2 9.599969280098305E-5 3 0.0 4 0.0 5 0.01334395729933664 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037439880192383385 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.799984640049152E-5 22-23 3.3599892480344065E-4 24-25 7.679975424078644E-4 26-27 0.0021599930880221185 28-29 0.002879990784029491 30-31 4.7999846400491515E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01012796759050371 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.799984640049152E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.919993856019661E-4 84-85 1.4399953920147455E-4 86-87 0.0 88-89 0.0 90-91 0.007391976345675694 92-93 0.002591991705626542 94-95 0.0 96-97 0.0 98-99 1.4399953920147455E-4 100-101 0.012047961446523371 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1041670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.63208992497339 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77752833224233 40.10475123882066 2 13.896609023899387 14.906083696439373 3 4.767312418863797 7.670427850468367 4 2.20755810693744 4.735838196234912 5 1.2010399621574 3.2207141626956157 6 0.7134235805971816 2.2957438577510727 7 0.452365922722134 1.6982930892498942 8 0.3468125932891363 1.4880227352316946 9 0.23718870975944878 1.1448833589906453 >10 1.257378998453401 12.364940779216555 >50 0.09568238506488562 3.4481880161757337 >100 0.04473587530376997 4.319286274818503 >500 0.0014548250521095461 0.500824945852369 >1k 5.455593945410798E-4 0.710908087695797 >5k 3.6370626302738654E-4 1.3910937103587808 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7805 0.7492776023116726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6458 0.6199660161087485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4246 0.4076146956329739 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.1515835149327522 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1464 0.14054355026063917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 9.599969280098303E-5 0.0 0.0 0.0 9.599969280098303E-5 14-15 1.9199938560196606E-4 0.0 0.0 4.7999846400491516E-5 9.599969280098303E-5 16-17 2.879990784029491E-4 0.0 0.0 1.9199938560196606E-4 9.599969280098303E-5 18-19 2.879990784029491E-4 0.0 0.0 1.9199938560196606E-4 9.599969280098303E-5 20-21 2.879990784029491E-4 0.0 0.0 1.9199938560196606E-4 9.599969280098303E-5 22-23 2.879990784029491E-4 0.0 0.0 4.3199861760442366E-4 9.599969280098303E-5 24-25 2.879990784029491E-4 0.0 0.0 8.159973888083558E-4 9.599969280098303E-5 26-27 3.3599892480344065E-4 0.0 0.0 0.001295995852813271 9.599969280098303E-5 28-29 4.3199861760442366E-4 0.0 0.0 0.0029279906304299826 9.599969280098303E-5 30-31 4.799984640049152E-4 0.0 0.0 0.006095980492862423 9.599969280098303E-5 32-33 5.759981568058982E-4 0.0 0.0 0.01166396267531944 9.599969280098303E-5 34-35 6.719978496068813E-4 0.0 0.0 0.017039945472174488 9.599969280098303E-5 36-37 6.719978496068813E-4 0.0 0.0 0.02212792919062659 9.599969280098303E-5 38-39 6.719978496068813E-4 0.0 0.0 0.027359912448280167 9.599969280098303E-5 40-41 6.719978496068813E-4 0.0 0.0 0.035951884953968144 9.599969280098303E-5 42-43 7.199976960073728E-4 0.0 0.0 0.0469918496260812 9.599969280098303E-5 44-45 7.679975424078643E-4 0.0 0.0 0.060575806157420295 9.599969280098303E-5 46-47 9.119970816093389E-4 0.0 0.0 0.07636775562318202 9.599969280098303E-5 48-49 9.599969280098304E-4 0.0 0.0 0.09067170985052847 9.599969280098303E-5 50-51 9.599969280098304E-4 0.0 0.0 0.11015964748912804 9.599969280098303E-5 52-53 9.599969280098304E-4 0.0 0.0 0.16099148482724857 9.599969280098303E-5 54-55 9.599969280098304E-4 0.0 0.0 0.23567924582641336 9.599969280098303E-5 56-57 9.599969280098304E-4 0.0 0.0 0.2973110486046444 9.599969280098303E-5 58-59 9.599969280098304E-4 0.0 0.0 0.39095874893200344 9.599969280098303E-5 60-61 0.0010079967744103218 0.0 0.0 0.5090383710772126 9.599969280098303E-5 62-63 0.0010559966208108134 0.0 0.0 0.5826221356091661 9.599969280098303E-5 64-65 0.0010559966208108134 0.0 0.0 0.644925936237004 9.599969280098303E-5 66-67 0.0011519963136117964 0.0 0.0 0.8218053702228153 9.599969280098303E-5 68-69 0.0011519963136117964 0.0 0.0 0.9883648372325209 9.599969280098303E-5 70-71 0.0012479960064127795 0.0 0.0 1.0730365662829877 9.599969280098303E-5 72-73 0.0013439956992137626 0.0 0.0 1.246508011174364 9.599969280098303E-5 74-75 0.0013439956992137626 0.0 0.0 1.5132431576218957 9.599969280098303E-5 76-77 0.0013919955456142541 0.0 0.0 1.7927942630583582 9.599969280098303E-5 78-79 0.0014399953920147457 0.0 0.0 2.043737460040128 9.599969280098303E-5 80-81 0.0014399953920147457 0.0 0.0 2.315032591895706 9.599969280098303E-5 82-83 0.0014399953920147457 0.0 0.0 2.560935805005424 9.599969280098303E-5 84-85 0.001487995238415237 0.0 0.0 2.86914281874298 9.599969280098303E-5 86-87 0.00158399493121622 0.0 0.0 3.430405022703927 9.599969280098303E-5 88-89 0.0016319947776167116 0.0 0.0 4.038467076905354 9.599969280098303E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5460 0.0 35.77632 1 GTATCAA 8170 0.0 32.05354 1 ATCAACG 10115 0.0 25.924543 3 TCAACGC 10155 0.0 25.86921 4 CAACGCA 10250 0.0 25.675793 5 TATCAAC 10290 0.0 25.529816 2 AACGCAG 10420 0.0 25.207674 6 GCGGTAT 95 0.0012223211 25.01456 1 TCCGACC 95 0.0012267773 24.998953 8 GAGTACT 6565 0.0 24.128845 12-13 GGACCGA 180 1.9514482E-7 23.749004 6 GGACCGT 120 1.711153E-4 23.749002 6 AGTACTT 6790 0.0 23.18938 12-13 GTACATG 5265 0.0 22.928827 1 GTACTTT 6845 0.0 22.899517 14-15 TACATGG 5195 0.0 22.860853 2 ACGCAGA 11630 0.0 22.503355 7 CGCAGAG 11705 0.0 22.359165 8 AGGACCG 235 4.642061E-9 22.236313 5 CCGCCGT 75 3.0345978E-5 22.166801 90-91 >>END_MODULE