FastQCFastQC Report
Thu 26 May 2016
SRR937667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74269
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11321.5241890963928422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10241.3787717621080129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8831.1889213534583742No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6020.8105669929580309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.803834708963363No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4490.6045591027211892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.4025905828811483No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2650.3568110517174056No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC2070.2787165573792565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.2787165573792565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.2679449029877876No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1790.24101576700911553No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1770.2383228534112483No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1650.22216537182404503No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.2140866310304434No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1580.2127401742315098No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1560.2100472606336426No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1510.20331497663897452No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1360.18311812465497043No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1320.17773229745923602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.1750393838613688No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1180.15888190227416554No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1150.15484253187736471No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1150.15484253187736471No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1140.1534960750784311No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA1080.14541733428482947No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1060.14272442068696226No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1060.14272442068696226No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT1060.14272442068696226No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA1050.14137796388802865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1020.13733859349122784No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA1000.13464567989336063No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC980.1319527662954934No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA980.1319527662954934No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA970.1306063094965598No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT940.126566939099759No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC930.1252204823008254No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG920.12387402550189178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.12387402550189178No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA900.12118111190402457No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG850.11444882790935652No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT830.11175591431148932No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT810.10906300071362211No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG800.1077165439146885No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT800.1077165439146885No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA790.1063700871157549No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC780.1050236303168213No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC770.10367717351788769No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA760.10233071671895407No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG750.10098425992002046No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGTG600.00392849231.6648715
GGAGTGT600.00392849231.6648716
ATGGGAT1252.1093547E-730.3982775
CCTAGAA804.455199E-429.6858183
ATGGGAG650.005811001629.2291135
CATGGGA3050.028.0311994
AGAAATG700.00834430527.1413156
AGTGTGA350.00837078827.14131534-35
GTACATG5100.024.230631
TCCTAGA1401.7513492E-523.7486522
ACATGGG5350.023.0828023
GAGTACT6500.021.92183312-13
GTCCTAG2405.664333E-921.784271
GTACTTT6350.021.69168514-15
TACATGG5700.021.6654382
GGAAGCA1150.00369226220.6510038
GGGAAGC1150.00369226220.6510037
TGGGAAG1200.004723970319.7905456
GAAGCAG1456.074448E-419.6540579
ATGGGAA2001.2591199E-518.9989225
Produced by FastQC (version 0.11.5)