##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74269 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.676608006032126 33.0 31.0 34.0 30.0 34.0 2 31.797277464352558 33.0 31.0 34.0 30.0 34.0 3 31.7834628175955 33.0 31.0 34.0 30.0 34.0 4 35.398658929028265 37.0 35.0 37.0 33.0 37.0 5 35.4024020789293 37.0 35.0 37.0 33.0 37.0 6 35.51683744227066 37.0 35.0 37.0 33.0 37.0 7 35.475528147679384 37.0 35.0 37.0 33.0 37.0 8 35.47947326610026 37.0 35.0 37.0 33.0 37.0 9 37.08290134511034 39.0 37.0 39.0 34.0 39.0 10-11 36.98127751821083 39.0 37.0 39.0 33.0 39.0 12-13 36.915637749262814 39.0 37.0 39.0 33.0 39.0 14-15 37.95143330326246 40.0 38.0 41.0 33.0 41.0 16-17 37.88109440008617 40.0 38.0 41.0 33.0 41.0 18-19 37.934575664139814 40.0 38.0 41.0 33.5 41.0 20-21 37.86835691876826 40.0 38.0 41.0 33.0 41.0 22-23 37.77189675369266 40.0 37.5 41.0 33.0 41.0 24-25 37.78284344746799 40.0 37.0 41.0 33.0 41.0 26-27 37.63983627085325 40.0 37.0 41.0 33.0 41.0 28-29 37.501272401674996 40.0 37.0 41.0 33.0 41.0 30-31 37.23448545153429 39.0 36.0 41.0 33.0 41.0 32-33 36.88241392774913 39.0 35.0 41.0 32.0 41.0 34-35 36.75258183091195 39.0 35.0 41.0 32.0 41.0 36-37 36.54607575165951 39.0 35.0 41.0 31.0 41.0 38-39 36.34210774347305 39.0 35.0 41.0 31.0 41.0 40-41 36.0774078013707 39.0 35.0 41.0 30.5 41.0 42-43 35.71151489854448 39.0 35.0 40.5 30.0 41.0 44-45 35.340640105562215 38.5 35.0 40.0 28.0 41.0 46-47 34.84346766483998 38.0 35.0 40.0 24.5 41.0 48-49 34.385685817770536 38.0 35.0 40.0 22.5 41.0 50-51 33.39953412594757 37.5 33.5 39.5 19.0 40.5 52-53 33.48475810903607 38.0 33.5 39.5 17.0 40.5 54-55 33.73256675059581 38.0 34.0 40.0 11.5 41.0 56-57 33.4736027144569 38.0 34.0 40.0 7.0 41.0 58-59 33.21776245809153 38.0 33.5 40.0 2.0 41.0 60-61 32.86723262734115 38.0 33.0 40.0 2.0 41.0 62-63 32.47138779302266 37.0 33.0 40.0 2.0 41.0 64-65 32.0717190213952 37.0 32.0 39.5 2.0 41.0 66-67 31.55469980745668 36.0 31.5 39.0 2.0 41.0 68-69 30.999979803148015 35.5 31.0 39.0 2.0 40.0 70-71 30.486521967442673 35.0 30.5 38.5 2.0 40.0 72-73 29.94997239763562 35.0 29.5 37.5 2.0 40.0 74-75 29.414439402711764 35.0 29.0 37.0 2.0 39.0 76-77 28.906643417845938 34.5 29.0 36.5 2.0 39.0 78-79 28.35304097268039 34.0 27.5 36.0 2.0 38.5 80-81 27.740295412621684 34.0 26.0 35.5 2.0 37.0 82-83 27.221532537128546 34.0 26.0 35.0 2.0 37.0 84-85 26.6810647780366 33.5 25.0 35.0 2.0 36.0 86-87 26.200675921313064 33.0 23.5 35.0 2.0 36.0 88-89 25.702958165587255 33.0 21.0 35.0 2.0 35.5 90-91 25.144750838169358 32.5 18.5 35.0 2.0 35.0 92-93 24.54645275956321 32.0 9.5 35.0 2.0 35.0 94-95 23.874375580659496 31.5 2.0 34.0 2.0 35.0 96-97 23.076243116239617 31.0 2.0 34.0 2.0 35.0 98-99 22.191371904832433 31.0 2.0 34.0 2.0 35.0 100-101 19.57053413941214 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32467631001903996 1101 2 0.3683051357535341 1101 3 0.7339065500519553 1101 4 0.42639543180299455 1101 5 0.40058701548356623 1101 6 0.1467907272936273 1101 7 0.6331208470691934 1101 8 -0.02896389465206539 1101 9 0.42828021661206606 1101 10-11 0.021804937360109022 1101 12-13 0.1852548904885296 1101 14-15 0.6096547593369266 1101 16-17 0.5207511952042054 1101 18-19 0.5255664664117248 1101 20-21 0.8081528979208699 1101 22-23 0.14059063380627634 1101 24-25 0.5034638477287956 1101 26-27 -0.46051419591756115 1101 28-29 0.33951988087924434 1101 30-31 0.6932613908098091 1101 32-33 0.3707146472566052 1101 34-35 1.1213937878485964 1101 36-37 1.025325654053951 1101 38-39 0.9474383831789623 1101 40-41 0.8674019264921924 1101 42-43 1.4625595763032635 1101 44-45 2.392117173269007 1101 46-47 2.5466535458132498 1101 48-49 2.0551931983853535 1101 50-51 1.4836818400793206 1101 52-53 2.211301495552668 1101 54-55 1.798444730498403 1101 56-57 1.5368835383421526 1101 58-59 1.2554406374987792 1101 60-61 1.6684062720132786 1101 62-63 1.6642191631541792 1101 64-65 1.6111774058979087 1101 66-67 0.891229451839564 1101 68-69 0.820810333460301 1101 70-71 0.5292937331004488 1101 72-73 1.7246037580269409 1101 74-75 1.3386685553602184 1101 76-77 -0.24430309054587696 1101 78-79 0.2771374496083894 1101 80-81 0.37752120227662544 1101 82-83 0.4253378476139211 1101 84-85 1.5839413835975193 1101 86-87 0.8304297748315541 1101 88-89 -0.18324131577009695 1101 90-91 -0.7925878525510051 1101 92-93 -0.7244371791237363 1101 94-95 0.2055966900284396 1101 96-97 -0.7646521563349182 1101 98-99 -1.0381949163604496 1101 100-101 -0.7059110721056321 1102 1 0.5565603679900555 1102 2 0.3703755291282711 1102 3 0.8781439551589258 1102 4 0.2676652730728364 1102 5 0.0010010941585179012 1102 6 0.2799860344019791 1102 7 0.37984272422585263 1102 8 0.3699318955480706 1102 9 0.45933611723318535 1102 10-11 0.630500589534023 1102 12-13 0.5558553045672099 1102 14-15 0.09378174346391432 1102 16-17 0.8637463642863352 1102 18-19 0.8947866182405662 1102 20-21 0.8930390262852583 1102 22-23 0.5460426696930867 1102 24-25 0.051082895347839496 1102 26-27 0.31553825404792946 1102 28-29 -0.02521441863765972 1102 30-31 0.4465395136531498 1102 32-33 0.27720187983079825 1102 34-35 0.36431994381823074 1102 36-37 0.5277411129911442 1102 38-39 0.5199021512949074 1102 40-41 1.5133646594114438 1102 42-43 1.4132152008719316 1102 44-45 0.9697569248218016 1102 46-47 1.5994485768691504 1102 48-49 1.9751379878953657 1102 50-51 2.0040407082642773 1102 52-53 1.131591350625122 1102 54-55 0.4896110520995123 1102 56-57 0.3705619372379445 1102 58-59 0.2471590639998169 1102 60-61 0.3985650021720133 1102 62-63 0.8005200603246436 1102 64-65 0.6563809945797594 1102 66-67 0.7404358010459191 1102 68-69 0.2535225487812127 1102 70-71 0.5945111244047929 1102 72-73 0.2601455109599975 1102 74-75 0.5498486795838211 1102 76-77 0.6649446665279655 1102 78-79 0.5138524254538019 1102 80-81 0.7595087799163771 1102 82-83 0.7300307392633378 1102 84-85 1.0211394512303684 1102 86-87 0.5481661447418382 1102 88-89 0.5638256269490185 1102 90-91 0.9800139417899203 1102 92-93 1.2414013301930353 1102 94-95 1.5510073180477626 1102 96-97 1.7998751038445207 1102 98-99 0.6588375198024217 1102 100-101 0.9017493833809098 1103 1 -2.0896094042666746 1103 2 -1.4602662928178987 1103 3 -2.045458529313123 1103 4 -1.6561442507366877 1103 5 -1.718460603564047 1103 6 -2.002415621912718 1103 7 -1.9748156608673213 1103 8 -1.484519450207621 1103 9 -2.0479102595784084 1103 10-11 -2.3853379197827493 1103 12-13 -2.321887966654323 1103 14-15 -2.44907539939323 1103 16-17 -2.432423407970397 1103 18-19 -2.2593541685089136 1103 20-21 -2.660894721126745 1103 22-23 -2.308615715400073 1103 24-25 -2.4393932951283475 1103 26-27 -2.133530068933446 1103 28-29 -2.5739721826128203 1103 30-31 -1.984516386967961 1103 32-33 -1.740396463854502 1103 34-35 -2.0659077994529937 1103 36-37 -1.9445156157873171 1103 38-39 -1.7716092358686595 1103 40-41 -2.808788549698285 1103 42-43 -2.3525197887760996 1103 44-45 -2.8126447314928953 1103 46-47 -1.9842988351391355 1103 48-49 -1.336870293678139 1103 50-51 -1.5940959376984551 1103 52-53 -2.462508028256863 1103 54-55 -2.0555235234698515 1103 56-57 -2.390100588641971 1103 58-59 -2.437416505358364 1103 60-61 -1.6752445216375165 1103 62-63 -1.78736813843312 1103 64-65 -1.423743229022726 1103 66-67 -1.8976594370493238 1103 68-69 -2.441094428444462 1103 70-71 -1.8540396002328876 1103 72-73 -1.7690470356238528 1103 74-75 -1.8327600160683524 1103 76-77 -2.2689062651490524 1103 78-79 -1.831592709121768 1103 80-81 -2.151050226294803 1103 82-83 -1.5516462793702068 1103 84-85 -0.4358998862437531 1103 86-87 -1.1322686378668543 1103 88-89 -1.0157809983097792 1103 90-91 -1.7529053128684673 1103 92-93 -2.286341941028496 1103 94-95 -1.1364668020350557 1103 96-97 -0.7725886642714244 1103 98-99 -0.6770838052493353 1103 100-101 0.7274222612277015 1104 1 -0.4038951185523878 1104 2 -0.2602662928178958 1104 3 -0.3454585293131238 1104 4 -0.2275728221652571 1104 5 -0.28988917499262357 1104 6 -0.330987050484147 1104 7 -0.10338708943874764 1104 8 -0.22737659306476132 1104 9 -0.19076740243555435 1104 10-11 -0.17819506263989382 1104 12-13 -0.11474510951146755 1104 14-15 -0.17764682796465792 1104 16-17 -0.18242340797039702 1104 18-19 0.1763601172053697 1104 20-21 -0.2537518639838936 1104 22-23 0.26281285602849636 1104 24-25 0.27489241915736784 1104 26-27 0.10932707392370133 1104 28-29 0.526027817387174 1104 30-31 0.4297693273177501 1104 32-33 -0.0046821781402144325 1104 34-35 -0.3659077994529909 1104 36-37 0.026912955641250846 1104 38-39 0.3855336212741989 1104 40-41 0.6412114503017179 1104 42-43 0.026051639795326764 1104 44-45 0.3373552685071033 1104 46-47 0.7942725934322965 1104 48-49 -0.05115600796384712 1104 50-51 -0.5298102234127384 1104 52-53 -0.37679374254258136 1104 54-55 -0.6269520948984209 1104 56-57 -0.8401005886419739 1104 58-59 -1.2302736482155083 1104 60-61 -1.4895302359232296 1104 62-63 -0.9016538527188338 1104 64-65 -0.966600371879867 1104 66-67 -1.1405165799064676 1104 68-69 -1.0839515713016041 1104 70-71 -1.1968967430900292 1104 72-73 -1.0976184641952784 1104 74-75 -0.9041885874969218 1104 76-77 -1.0760491222919093 1104 78-79 -0.9387355662646257 1104 80-81 -0.45819308343765996 1104 82-83 0.0983537206297953 1104 84-85 0.14981439947053588 1104 86-87 -0.18226863786685854 1104 88-89 -0.07292385545263969 1104 90-91 0.1828089728458231 1104 92-93 0.35651520182864616 1104 94-95 -0.15789537346362437 1104 96-97 -0.10116009284285354 1104 98-99 0.7943447661792327 1104 100-101 0.3559936897991314 1105 1 0.7325710468611426 1105 2 0.554395361317443 1105 3 0.27860162106281905 1105 4 0.3904722906166995 1105 5 0.37552435884196456 1105 6 0.7355543028993097 1105 7 0.672176820335693 1105 8 0.7030745347547835 1105 9 1.1494581614742216 1105 10-11 0.8244365163074789 1105 12-13 0.47566842432311773 1105 14-15 0.7582554276744418 1105 16-17 1.1055465168416347 1105 18-19 0.7694052299873206 1105 20-21 0.5979398653394199 1105 22-23 0.8120609763292421 1105 24-25 1.3559074567513534 1105 26-27 0.9860188032470134 1105 28-29 0.9498999978383083 1105 30-31 1.2331527859643714 1105 32-33 1.0058441376492553 1105 34-35 0.7746937043064079 1105 36-37 0.4895069406036541 1105 38-39 1.0943682077403665 1105 40-41 0.06132423225660233 1105 42-43 -0.37902354817460093 1105 44-45 0.19506203542439238 1105 46-47 -0.4602386847631905 1105 48-49 -0.21412593277587888 1105 50-51 -0.2865771407059725 1105 52-53 -0.5474704342719008 1105 54-55 -0.8737566061766202 1105 56-57 -0.5139727690931011 1105 58-59 -0.7257623700200213 1105 60-61 -0.9654700855472882 1105 62-63 -0.9437591158767269 1105 64-65 -0.8470514996994183 1105 66-67 -2.070967707726016 1105 68-69 -2.1986132254369437 1105 70-71 -1.027723810759202 1105 72-73 -1.1714906446464077 1105 74-75 -0.8117073844894023 1105 76-77 -1.1125152877054418 1105 78-79 -0.7857280474676323 1105 80-81 -1.1046216548662322 1105 82-83 -0.9676237229792264 1105 84-85 -0.07029838248435283 1105 86-87 0.04931030950156412 1105 88-89 0.4387302798857071 1105 90-91 0.5835608525450695 1105 92-93 1.2006881341594742 1105 94-95 1.506766280671716 1105 96-97 0.8721481778338358 1105 98-99 1.9535552924950217 1105 100-101 2.422535043182588 1106 1 -0.4599390745963454 1106 2 -0.6822443147959198 1106 3 -0.7586453424999355 1106 4 -0.9990013935938293 1106 5 -1.6536254387288878 1106 6 -1.0573606768577761 1106 7 -0.8923980784497374 1106 8 -0.318585384273554 1106 9 -0.5248333365014872 1106 10-11 -0.6320412164860443 1106 12-13 -0.9015582963246587 1106 14-15 -0.4908336411514682 1106 16-17 -0.4989069244539124 1106 18-19 -0.8335299926847384 1106 20-21 -1.066938677170704 1106 22-23 -0.3492750560594189 1106 24-25 -0.2668658226008702 1106 26-27 -0.7346289700323396 1106 28-29 -0.9074886661293036 1106 30-31 -1.0427581452097243 1106 32-33 -0.7239129473709909 1106 34-35 0.3329932994481126 1106 36-37 -0.19176836304006883 1106 38-39 0.17564351138408796 1106 40-41 0.716486175576442 1106 42-43 0.7886890024326902 1106 44-45 0.847794828946661 1106 46-47 1.6371297362894452 1106 48-49 1.2114813546735164 1106 50-51 0.7575524139498953 1106 52-53 0.9369425211936928 1106 54-55 0.49942153147519974 1106 56-57 -0.014276412817800122 1106 58-59 0.11148459354273399 1106 60-61 0.6088214124284193 1106 62-63 0.6021923011273245 1106 64-65 1.1476853424058469 1106 66-67 0.8506922113023307 1106 68-69 1.6226418352918017 1106 70-71 1.1421142459209612 1106 72-73 1.9320518654750494 1106 74-75 1.1595476762393382 1106 76-77 1.7107640645212783 1106 78-79 1.840385312856256 1106 80-81 1.766532191287613 1106 82-83 0.6203317426078172 1106 84-85 0.4668473665035009 1106 86-87 0.7023467467485283 1106 88-89 0.9836695511407676 1106 90-91 1.5064353464721947 1106 92-93 1.460910806224252 1106 94-95 1.381665066096815 1106 96-97 2.0427959511131917 1106 98-99 1.513575535410002 1106 100-101 0.7834662172716556 1107 1 0.05194903729176659 1107 2 0.2384350058833995 1107 3 0.3168791330245426 1107 4 0.06853107393863667 1107 5 0.5217991366956909 1107 6 0.23914281964572126 1107 7 -0.017672803724458674 1107 8 0.28561041992225 1107 9 0.6014403897722431 1107 10-11 0.39193480748998155 1107 12-13 0.3281120333456684 1107 14-15 0.7885869382691126 1107 16-17 0.7877064621594769 1107 18-19 0.871165312010568 1107 20-21 0.9748195645875413 1107 22-23 0.6550206482362881 1107 24-25 0.8281391724041214 1107 26-27 1.1404959050925285 1107 28-29 1.139664181023548 1107 30-31 1.0713277688761949 1107 32-33 0.7466165231584867 1107 34-35 1.047079213534026 1107 36-37 0.8139259426542438 1107 38-39 0.808910244650825 1107 40-41 0.9626400217302873 1107 42-43 0.7987789125226001 1107 44-45 0.35099163214346873 1107 46-47 0.4059609051206081 1107 48-49 -0.35894821575605107 1107 50-51 -0.534355677958203 1107 52-53 -0.8579625737114043 1107 54-55 0.05746348951716129 1107 56-57 0.15015915161776405 1107 58-59 0.1320640141221503 1107 60-61 -0.2875821839751822 1107 62-63 -0.0010045020694846585 1107 64-65 0.7035294982499956 1107 66-67 0.05428861489872716 1107 68-69 0.6303341429841112 1107 70-71 0.9362201400268546 1107 72-73 1.3400438734670566 1107 74-75 1.3841231008147652 1107 76-77 1.1590158127730241 1107 78-79 1.3132124856834295 1107 80-81 1.5983004230558464 1107 82-83 1.2983537206297946 1107 84-85 1.190723490379625 1107 86-87 1.3333157777175586 1107 88-89 1.173180040651257 1107 90-91 1.5393024793393266 1107 92-93 2.0591126044260477 1107 94-95 1.8498968343285824 1107 96-97 2.72091782923507 1107 98-99 3.235253857088324 1107 100-101 4.3287209625264005 1108 1 -0.07792109257836088 1108 2 -0.021305253856858997 1108 3 -0.6181858020403936 1108 4 -0.42497541956785057 1108 5 0.04127965617620788 1108 6 -0.04657146606856344 1108 7 -0.4202702063218666 1108 8 -0.8442597099478775 1108 9 -0.6063518180199665 1108 10-11 -0.2768963613411941 1108 12-13 0.016423721657368162 1108 14-15 -0.07504942536726134 1108 16-17 0.08640776086077295 1108 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-1.5947453023730347 2316 94-95 -0.5957104995140448 2316 96-97 -0.35494160544789466 2316 98-99 -1.8497728808795877 2316 100-101 -2.6482079908731357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 516.0 3 96.0 4 75.0 5 68.0 6 66.0 7 81.0 8 95.0 9 111.0 10 137.0 11 218.0 12 306.0 13 449.0 14 551.0 15 800.0 16 1030.0 17 1370.0 18 1112.0 19 677.0 20 706.0 21 755.0 22 756.0 23 854.0 24 946.0 25 972.0 26 1038.0 27 1151.0 28 1318.0 29 1511.0 30 1857.0 31 2247.0 32 2793.0 33 3616.0 34 5004.0 35 7264.0 36 9156.0 37 13306.0 38 10291.0 39 970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.574816655636745 24.321659621020785 16.444942666900772 18.6585810564417 2 14.106752342125867 25.326007721590756 39.253219579362295 21.314020356921084 3 17.741151696768444 30.063983153802543 32.54906724980427 19.64579789962474 4 10.609230935378053 21.345356858404095 41.07887738434332 26.96653482187454 5 9.67990171592121 42.20140135815637 35.68873108233992 12.42996584358251 6 24.232123345878353 42.54110854814995 17.8085333117505 15.418234794221197 7 21.925919930937738 38.95813100248193 23.470378763353835 15.645570303226503 8 22.699005814031917 35.863538870378115 21.57531936706641 19.86213594852356 9 24.41524806431597 19.43103029649014 24.18458467100116 31.96913696819273 10-11 21.473137499578428 30.258001416478365 28.357222353377626 19.911638730565578 12-13 24.024901023154175 29.73082344688973 28.57412640708991 17.67014912286618 14-15 21.51605897508532 31.586472960759714 28.372654553302844 18.524813510852116 16-17 16.481626375998275 34.63805849341679 29.96101338225772 18.919301748327218 18-19 16.09990018075377 33.03207704966682 34.020827150834975 16.847195618744433 20-21 17.520704631902667 31.353017346030377 33.63524778116486 17.4910302409021 22-23 17.641068526224213 30.632119180733618 33.96974622164973 17.757066071392444 24-25 18.077278460096178 31.687675778483705 33.385940419103115 16.849105342317007 26-27 16.89382852595445 31.65856257460598 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52-53 17.039221110443894 33.19254306360527 31.8660558258159 17.902180000134944 54-55 17.557323981656324 32.04410574588616 31.642837874291878 18.755732398165634 56-57 18.03377075269397 32.673607833088326 30.704546374094704 18.588075040122998 58-59 18.026164947063187 31.261716906062446 31.987996493357613 18.724121653516757 60-61 17.55891972082673 32.60123402677097 31.894534542634613 17.94531170976769 62-63 17.14331955411994 32.47398695807567 32.56704722471357 17.815646263090816 64-65 17.251889865197484 32.281797276975674 32.11253548765603 18.353777370170814 66-67 17.289978016642614 32.21910529084116 30.937192334147035 19.553724358369184 68-69 16.921925263519938 31.3843311010851 32.317019712572744 19.376723922822208 70-71 18.254894159377972 31.312504636217113 31.9126840156721 18.519917188732812 72-73 17.631310985683747 30.326448315159855 32.85859750628823 19.183643192868175 74-75 17.195012576453372 32.352167668062556 31.31368304640138 19.139136709082692 76-77 17.32058319733488 30.756780815451222 32.318627517095344 19.604008470118554 78-79 17.00767435900894 30.687320448322836 32.57960967319909 19.72539551946913 80-81 18.01691234979163 31.190068377682174 31.315494895275602 19.477524377250596 82-83 17.89675983681176 30.53575643143734 32.028726524832265 19.538757206918643 84-85 17.432584610612487 30.242688658570298 31.70664275069624 20.618083980120975 86-87 17.79048441040026 30.963426475347934 31.363280828568346 19.882808285683463 88-89 18.25151719487525 30.24207687120701 30.951449763991906 20.554956169925827 90-91 17.963950720513562 29.139497090298523 31.966931225850825 20.929620963337086 92-93 19.06206803546745 30.323994470853982 31.402852230201272 19.211085263477294 94-95 18.549686781434804 29.09257648399517 31.99751852676013 20.360218207809897 96-97 18.125177003681678 29.178972636916562 31.68736766867608 21.008482690725682 98-99 18.649394088570293 30.04740742738841 30.64623808913675 20.656960394904544 100-101 18.939363294365112 28.567479740571443 31.732804767876168 20.760352197187277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 621.0 1 392.0 2 170.5 3 186.0 4 157.0 5 159.0 6 242.0 7 405.0 8 567.5 9 600.0 10 471.0 11 319.5 12 315.0 13 333.0 14 303.0 15 280.0 16 279.0 17 260.5 18 253.0 19 262.0 20 288.0 21 312.5 22 321.0 23 357.5 24 415.0 25 510.0 26 709.5 27 946.5 28 990.0 29 1078.5 30 1374.5 31 1688.0 32 2224.5 33 2768.0 34 3087.5 35 3502.0 36 4164.0 37 4659.5 38 4370.5 39 3642.0 40 3095.0 41 2691.5 42 2145.5 43 2050.5 44 2165.5 45 2006.0 46 1783.5 47 1978.0 48 2980.0 49 2835.5 50 1647.0 51 934.0 52 586.5 53 488.0 54 417.0 55 382.5 56 352.5 57 277.0 58 251.5 59 249.0 60 229.0 61 196.0 62 156.0 63 120.0 64 90.0 65 76.5 66 63.0 67 58.5 68 51.0 69 39.0 70 32.5 71 27.5 72 24.5 73 32.0 74 29.0 75 13.5 76 9.0 77 8.5 78 7.5 79 5.5 80 2.5 81 1.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3069921501568622 2 0.2571732485963188 3 0.25178742140058435 4 0.2585197053952524 5 0.26659844618885403 6 0.18311812465497043 7 0.17907875425816963 8 0.18581103825283768 9 0.18177166785603682 10-11 0.19052363704910527 12-13 0.18244489625550364 14-15 0.18311812465497043 16-17 0.18985040864963848 18-19 0.18177166785603682 20-21 0.1763858406603024 22-23 0.174366155461902 24-25 0.18109843945657003 26-27 0.174366155461902 28-29 0.18850395185070487 30-31 0.19388977904643928 32-33 0.19725592104377332 34-35 0.1925433222475057 36-37 0.16696064306776717 38-39 0.16696064306776717 40-41 0.16696064306776717 42-43 0.1750393838613688 44-45 0.1804252110571032 46-47 0.18783072345123808 48-49 0.18513780985337086 50-51 0.1911968654485721 52-53 0.2201456866256446 54-55 0.1736929270624352 56-57 0.16426772946989995 58-59 0.16696064306776717 60-61 0.16359450107043316 62-63 0.16628741466830035 64-65 0.16628741466830035 66-67 0.16426772946989995 68-69 0.1730196986629684 70-71 0.167633871467234 72-73 0.16494095786936674 74-75 0.16494095786936674 76-77 0.1696535566656344 78-79 0.1696535566656344 80-81 0.16426772946989995 82-83 0.16359450107043316 84-85 0.16224804427149955 86-87 0.15753544547523193 88-89 0.16022835907309912 90-91 0.16224804427149955 92-93 0.15686221707576511 94-95 0.16090158747256594 96-97 0.15888190227416554 98-99 0.167633871467234 100-101 0.14272442068696226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74269.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.14521536576499 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31997548080606 26.119915442513026 2 9.263657957244655 6.511465079642919 3 4.244885449390852 4.4756223996553075 4 2.513217377978699 3.5331026404017827 5 1.490307256148954 2.6188584739258642 6 1.1608305876944296 2.4478584604612963 7 0.8811585319132633 2.167795446283106 8 0.6474599647536587 1.8204095921582355 9 0.5976553520803004 1.890425345702783 >10 4.451766148187878 29.708222811671085 >50 0.31032104819554057 7.258748603051071 >100 0.09960922534671672 5.741291790652897 >500 0.011493372155390391 2.803323055379768 >1k 0.007662248103593594 2.902960858500855 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1132 1.5241890963928422 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1024 1.3787717621080129 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 883 1.1889213534583742 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 602 0.8105669929580309 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 597 0.803834708963363 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 449 0.6045591027211892 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.4025905828811483 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 265 0.3568110517174056 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 207 0.2787165573792565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.2787165573792565 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.2679449029877876 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 179 0.24101576700911553 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 177 0.2383228534112483 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 165 0.22216537182404503 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 159 0.2140866310304434 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 158 0.2127401742315098 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 156 0.2100472606336426 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 151 0.20331497663897452 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 136 0.18311812465497043 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 132 0.17773229745923602 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.1750393838613688 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 118 0.15888190227416554 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 115 0.15484253187736471 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 115 0.15484253187736471 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 114 0.1534960750784311 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 108 0.14541733428482947 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 106 0.14272442068696226 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 106 0.14272442068696226 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 106 0.14272442068696226 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 105 0.14137796388802865 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 102 0.13733859349122784 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 100 0.13464567989336063 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 98 0.1319527662954934 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 98 0.1319527662954934 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 97 0.1306063094965598 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 94 0.126566939099759 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 93 0.1252204823008254 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 92 0.12387402550189178 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.12387402550189178 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 90 0.12118111190402457 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 85 0.11444882790935652 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 83 0.11175591431148932 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 81 0.10906300071362211 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 80 0.1077165439146885 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 80 0.1077165439146885 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 79 0.1063700871157549 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 78 0.1050236303168213 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 77 0.10367717351788769 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 76 0.10233071671895407 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 75 0.10098425992002046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0013464567989336061 0.0 26-27 0.0 0.0 0.0 0.0053858271957344245 0.0 28-29 0.0 0.0 0.0 0.024909450780271715 0.0 30-31 0.0 0.0 0.0 0.04645275956320942 0.0 32-33 0.0 0.0 0.0 0.09155906232748523 0.0 34-35 0.0 0.0 0.0 0.14272442068696226 0.0 36-37 0.0 0.0 0.0 0.1959094642448397 0.0 38-39 0.0 0.0 0.0 0.2766968721808561 0.0 40-41 0.0 0.0 0.0 0.3467126257254036 0.0 42-43 0.0 0.0 0.0 0.4160551508704843 0.0 44-45 0.0 0.0 0.0 0.4598149968358265 0.0 46-47 0.0 0.0 0.0 0.5244449231846395 0.0 48-49 0.0 0.0 0.0 0.5769567383430503 0.0 50-51 0.0 0.0 0.0 0.6469724918875979 0.0 52-53 0.0 0.0 0.0 0.7237205294268133 0.0 54-55 0.0 0.0 0.0 0.8395158141351036 0.0 56-57 0.0 0.0 0.0 1.005803228803404 0.0 58-59 0.0 0.0 0.0 1.1855552114610401 0.0 60-61 0.0 0.0 0.0 1.3780985337085458 0.0 62-63 0.0 0.0 0.0 1.5369804359827115 0.0 64-65 0.0 0.0 0.0 1.7611654930051568 0.0 66-67 0.0 0.0 0.0 2.0499804763764153 0.0 68-69 0.0 0.0 0.0 2.375823021718348 0.0 70-71 0.0 0.0 0.0 2.7245553326421525 0.0 72-73 0.0 0.0 0.0 3.032220711198481 0.0 74-75 0.0 0.0 0.0 3.3796065653233516 0.0 76-77 0.0 0.0 0.0 3.7788310062071657 0.0 78-79 0.0 0.0 0.0 4.188153873082982 0.0 80-81 0.0 0.0 0.0 4.572567289178527 0.0 82-83 0.0 0.0 0.0 4.961693304070339 0.0 84-85 0.0 0.0 0.0 5.496236653246981 0.0 86-87 0.0 0.0 0.0 6.103488669566037 0.0 88-89 0.0 0.0 0.0 6.754500531850436 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGAGTG 60 0.003928492 31.664871 5 GGAGTGT 60 0.003928492 31.664871 6 ATGGGAT 125 2.1093547E-7 30.398277 5 CCTAGAA 80 4.455199E-4 29.685818 3 ATGGGAG 65 0.0058110016 29.229113 5 CATGGGA 305 0.0 28.031199 4 AGAAATG 70 0.008344305 27.141315 6 AGTGTGA 35 0.008370788 27.141315 34-35 GTACATG 510 0.0 24.23063 1 TCCTAGA 140 1.7513492E-5 23.748652 2 ACATGGG 535 0.0 23.082802 3 GAGTACT 650 0.0 21.921833 12-13 GTCCTAG 240 5.664333E-9 21.78427 1 GTACTTT 635 0.0 21.691685 14-15 TACATGG 570 0.0 21.665438 2 GGAAGCA 115 0.003692262 20.651003 8 GGGAAGC 115 0.003692262 20.651003 7 TGGGAAG 120 0.0047239703 19.790545 6 GAAGCAG 145 6.074448E-4 19.654057 9 ATGGGAA 200 1.2591199E-5 18.998922 5 >>END_MODULE