##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937667_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74269 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.898450228224426 31.0 31.0 34.0 30.0 34.0 2 32.167768517147124 33.0 31.0 34.0 30.0 34.0 3 32.2160389933889 34.0 31.0 34.0 30.0 34.0 4 35.936204876866526 37.0 35.0 37.0 35.0 37.0 5 35.84327242860413 37.0 35.0 37.0 35.0 37.0 6 35.91573873352274 37.0 35.0 37.0 35.0 37.0 7 35.85100109063001 37.0 35.0 37.0 35.0 37.0 8 35.84169707414937 37.0 35.0 37.0 35.0 37.0 9 37.46423137513633 39.0 37.0 39.0 34.0 39.0 10-11 37.39281530652089 39.0 37.0 39.0 34.0 39.0 12-13 37.32382959242753 39.0 37.0 39.0 34.0 39.0 14-15 38.33380683730762 40.0 38.0 41.0 33.0 41.0 16-17 38.306231402065464 40.0 38.0 41.0 33.5 41.0 18-19 38.38514050276697 40.0 38.0 41.0 34.0 41.0 20-21 38.34074108982213 40.0 38.0 41.0 34.0 41.0 22-23 38.24131871978888 40.0 37.5 41.0 34.0 41.0 24-25 38.23323997899527 40.0 37.0 41.0 34.0 41.0 26-27 38.01537653664382 40.0 37.0 41.0 33.0 41.0 28-29 37.80554470909801 40.0 37.0 41.0 33.0 41.0 30-31 37.680546392169006 39.0 36.5 41.0 33.0 41.0 32-33 37.40890546526815 39.0 35.0 41.0 33.0 41.0 34-35 37.15014339764909 39.0 35.0 41.0 33.0 41.0 36-37 36.90704735488562 39.0 35.0 41.0 32.5 41.0 38-39 36.66642879263219 39.0 35.0 41.0 31.5 41.0 40-41 36.44462696414385 39.0 35.0 41.0 31.0 41.0 42-43 36.10817433922632 39.0 35.0 41.0 30.5 41.0 44-45 35.65615532725633 38.5 35.0 40.0 29.5 41.0 46-47 35.0569416580269 38.0 35.0 40.0 26.0 41.0 48-49 34.54930051569295 38.0 35.0 40.0 23.5 41.0 50-51 34.15371824045026 38.0 34.0 40.0 21.0 41.0 52-53 33.90524983505904 38.0 34.0 40.0 18.5 41.0 54-55 33.63966796375338 38.0 34.0 40.0 14.0 41.0 56-57 33.27463679327849 38.0 33.0 40.0 7.5 41.0 58-59 32.986959565902325 38.0 33.0 40.0 2.0 41.0 60-61 32.571133312687664 37.0 33.0 40.0 2.0 41.0 62-63 32.142960050626776 37.0 32.0 39.5 2.0 41.0 64-65 31.681953439523895 36.0 31.5 39.0 2.0 40.5 66-67 31.168616784930457 36.0 31.0 39.0 2.0 40.0 68-69 30.671390485936257 35.0 30.5 38.5 2.0 40.0 70-71 30.23087021502915 35.0 29.5 38.0 2.0 40.0 72-73 29.68595914850072 35.0 29.0 37.0 2.0 39.0 74-75 29.148130444734683 34.0 29.0 37.0 2.0 39.0 76-77 27.891367865462037 32.5 27.0 35.0 2.0 37.5 78-79 28.445098224023482 34.0 28.5 36.0 2.0 38.0 80-81 28.201571315084358 34.0 28.0 35.5 2.0 37.0 82-83 27.758701477063106 34.0 26.5 35.0 2.0 37.0 84-85 27.31193364660895 34.0 26.0 35.0 2.0 36.0 86-87 26.969677792888014 33.5 26.0 35.0 2.0 36.0 88-89 26.46435928853223 33.0 25.0 35.0 2.0 35.5 90-91 25.87930361254359 33.0 23.5 35.0 2.0 35.0 92-93 25.43766578249337 32.0 20.0 35.0 2.0 35.0 94-95 24.950167633871466 32.0 18.5 34.0 2.0 35.0 96-97 24.292908212040018 32.0 4.5 34.0 2.0 35.0 98-99 23.435780742974863 31.0 2.0 34.0 2.0 35.0 100-101 20.817339670656665 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04709193032918435 1101 2 0.15711370991636642 1101 3 0.23368362695767786 1101 4 -0.19920429203072842 1101 5 0.11327421893279421 1101 6 -0.0882009344125052 1101 7 -0.027495770369149852 1101 8 -0.4567711768611957 1101 9 0.42561103624645824 1101 10-11 -0.262045644904795 1101 12-13 0.5422504782760242 1101 14-15 -0.21965548708406857 1101 16-17 -0.3048106758181177 1101 18-19 0.005834248570188549 1101 20-21 0.02961125590790914 1101 22-23 0.14956467072868662 1101 24-25 0.28215256685457035 1101 26-27 0.3220469372402306 1101 28-29 -0.07227128708241537 1101 30-31 -0.0037531844065270548 1101 32-33 -0.9558526461056047 1101 34-35 -0.6180149764681317 1101 36-37 -0.7105370942642963 1101 38-39 -0.32335229157716583 1101 40-41 0.021525005520572904 1101 42-43 0.442448265348915 1101 44-45 0.3044023091796433 1101 46-47 0.020819384182459544 1101 48-49 0.013382199449608834 1101 50-51 0.005027460410644835 1101 52-53 0.3027354277164065 1101 54-55 -0.7160865002878793 1101 56-57 -0.783827433934043 1101 58-59 -0.3842311602907458 1101 60-61 0.05042062361903277 1101 62-63 -0.6241949586189257 1101 64-65 -0.40112409978159036 1101 66-67 -1.1337384457708843 1101 68-69 -0.14109742888260968 1101 70-71 0.35073032879695987 1101 72-73 0.48390409044129257 1101 74-75 0.32551028707024443 1101 76-77 -0.6844185552997963 1101 78-79 -0.5678680610735931 1101 80-81 -0.8001183182381446 1101 82-83 -0.8651694699719137 1101 84-85 -0.8829441866780066 1101 86-87 -0.5975261979520923 1101 88-89 -1.5421919605965826 1101 90-91 -2.341366470655796 1101 92-93 -2.7888880813743597 1101 94-95 -2.3357456546240414 1101 96-97 -2.2250910606750303 1101 98-99 -1.6636525329021445 1101 100-101 -1.4051165399001952 1102 1 0.44115680265492685 1102 2 0.47595428962650743 1102 3 0.25162703620543425 1102 4 0.43433608064007956 1102 5 0.5073390912585367 1102 6 0.2865402664156562 1102 7 0.2789224490925477 1102 8 0.26234545529891307 1102 9 0.41939985612223296 1102 10-11 0.6782580127901596 1102 12-13 0.7865568964954761 1102 14-15 0.8880049684024769 1102 16-17 0.5022976747684922 1102 18-19 0.49755267507121914 1102 20-21 0.6210536299589791 1102 22-23 0.8120905506458698 1102 24-25 0.3004410416647758 1102 26-27 0.7250835141898477 1102 28-29 0.28245611112324553 1102 30-31 0.05214743671148625 1102 32-33 0.17941305437748412 1102 34-35 -0.06936073216171224 1102 36-37 0.37193357516289893 1102 38-39 0.41508801208052404 1102 40-41 0.37590733816377764 1102 42-43 0.6846842901936299 1102 44-45 0.6732773954460427 1102 46-47 -0.4584766820701276 1102 48-49 -0.6974528592115377 1102 50-51 -0.08123893020356832 1102 52-53 -0.1223853452304553 1102 54-55 0.265279959339459 1102 56-57 0.6319765688264809 1102 58-59 0.16304282176584906 1102 60-61 -0.02342340674673693 1102 62-63 0.31610869907603956 1102 64-65 0.16788901270978585 1102 66-67 0.09124430095099356 1102 68-69 0.24641119775644782 1102 70-71 -0.02953192103050739 1102 72-73 0.019790908936808904 1102 74-75 0.41143161212200496 1102 76-77 0.07196515760565703 1102 78-79 0.45835692167312914 1102 80-81 0.48835649197579656 1102 82-83 0.35946821118750805 1102 84-85 0.7409343502440073 1102 86-87 0.3921218351742013 1102 88-89 0.4926596612115617 1102 90-91 0.6627743160936852 1102 92-93 1.0737758247678073 1102 94-95 1.7512108671150877 1102 96-97 1.3166618723822516 1102 98-99 2.8694737610937118 1102 100-101 3.1759738672771682 1103 1 0.4359808192180701 1103 2 0.23806609086874175 1103 3 0.5305090237830754 1103 4 -0.12777572060215903 1103 5 0.045020250678824425 1103 6 0.026084779873208674 1103 7 0.13440899153560792 1103 8 0.24640342631340673 1103 9 0.5922777029131225 1103 10-11 0.22763689477773852 1103 12-13 0.5136790497045922 1103 14-15 0.9136778462492643 1103 16-17 0.2626496416422057 1103 18-19 0.28440567714161347 1103 20-21 0.1716747479714016 1103 22-23 0.6471837183477334 1103 24-25 0.6496128843148838 1103 26-27 0.5283961435894327 1103 28-29 0.9594747446636163 1103 30-31 0.5986277679744276 1103 32-33 0.12113148087852466 1103 34-35 0.4438897854366317 1103 36-37 0.8902565565293585 1103 38-39 0.28775881953394133 1103 40-41 -0.06974483574926893 1103 42-43 0.36863874153939236 1103 44-45 0.948846753624089 1103 46-47 -0.029180615817537614 1103 48-49 0.43639807246547946 1103 50-51 0.4629639683471538 1103 52-53 0.4900370150179967 1103 54-55 0.026770642569267977 1103 56-57 -0.6282718783784915 1103 58-59 -0.6215327475923331 1103 60-61 -1.1202142970158846 1103 62-63 -1.3868933713173384 1103 64-65 -0.9979494966069851 1103 66-67 -0.5797701918026306 1103 68-69 -0.6918910796762638 1103 70-71 -0.45799982993320043 1103 72-73 -0.8795879730507714 1103 74-75 -1.0078230462630877 1103 76-77 -0.852672523553764 1103 78-79 -0.6448521880577189 1103 80-81 -0.2255151436349685 1103 82-83 -0.281836136638578 1103 84-85 -0.013102916836732703 1103 86-87 0.1834261830002859 1103 88-89 -0.4675887859934065 1103 90-91 0.02291924362991793 1103 92-93 0.3769849344986582 1103 94-95 0.5880638691854863 1103 96-97 0.24554385995989136 1103 98-99 1.6601569909073781 1103 100-101 1.0774231426394891 1104 1 0.3216951049323562 1104 2 0.2523518051544613 1104 3 0.4876518809259309 1104 4 0.1865099936835577 1104 5 0.5450202506788244 1104 6 0.28322763701606846 1104 7 0.520123277249894 1104 8 0.5892605691705484 1104 9 0.23513484577026844 1104 10-11 0.2347797519205983 1104 12-13 0.11367904970459364 1104 14-15 0.4208207033921241 1104 16-17 0.49836392735648616 1104 18-19 0.8415485342844775 1104 20-21 0.6002461765428251 1104 22-23 0.354326575490596 1104 24-25 0.2638985986005977 1104 26-27 0.17839614358943834 1104 28-29 0.4737604589493287 1104 30-31 0.2486277679744262 1104 32-33 0.6782743380213816 1104 34-35 0.629604071150915 1104 36-37 0.31168512795792935 1104 38-39 0.5234731052482289 1104 40-41 0.5016837356793005 1104 42-43 0.8257815986822479 1104 44-45 0.8488467536240947 1104 46-47 0.15653366989674566 1104 48-49 0.2792552153226282 1104 50-51 -0.4656074602242768 1104 52-53 -0.2742486992677229 1104 54-55 -0.016086500287876504 1104 56-57 0.1860138359072252 1104 58-59 0.34989582383624196 1104 60-61 -0.5130714398730305 1104 62-63 -0.1797505141744793 1104 64-65 -0.6479494966069836 1104 66-67 -1.4226273346597758 1104 68-69 -1.099033936819115 1104 70-71 -2.27942840136177 1104 72-73 -2.851016544479343 1104 74-75 -1.9221087605488059 1104 76-77 -1.895529666410905 1104 78-79 -1.530566473772005 1104 80-81 -1.4255151436349713 1104 82-83 -1.9389789937814363 1104 84-85 -1.6416743454081661 1104 86-87 -1.459430959856853 1104 88-89 -1.6890173574219816 1104 90-91 -2.0127950420843668 1104 92-93 -2.015872208358484 1104 94-95 -2.433364702243086 1104 96-97 -2.1830275686115392 1104 98-99 -2.1612715805211913 1104 100-101 -2.908291143074795 1105 1 0.17094322523311334 1105 2 -0.05779857078538697 1105 3 -0.2134759386229419 1105 4 0.23124683578881644 1105 5 -0.15761132826854407 1105 6 -0.11639642313430443 1105 7 -0.13965115884032997 1105 8 -0.1562281526339646 1105 9 0.007691236747710661 1105 10-11 0.3124113308679668 1105 12-13 -0.37410290518262457 1105 14-15 0.3890537860988914 1105 16-17 0.09535640855948913 1105 18-19 0.2351575568408606 1105 20-21 0.039531890828541805 1105 22-23 -0.014282447065802728 1105 24-25 -0.10621418335428956 1105 26-27 -0.47724295415492435 1105 28-29 0.04218151158090677 1105 30-31 0.1544548356435982 1105 32-33 -0.1437181431816228 1105 34-35 -0.39840344764608204 1105 36-37 -0.595457729184929 1105 38-39 -0.44532388723297345 1105 40-41 0.30525516425073107 1105 42-43 -0.08324095770872475 1105 44-45 -0.5434464794586162 1105 46-47 -0.48669941281002593 1105 48-49 -0.5120981681360206 1105 50-51 -0.638351821126534 1105 52-53 -0.3515043383654657 1105 54-55 -0.47642484615253267 1105 56-57 0.18432210658391313 1105 58-59 -0.32303650699082453 1105 60-61 -0.0487857255873152 1105 62-63 0.5072795610134904 1105 64-65 0.4078775710621869 1105 66-67 -0.4784544023289463 1105 68-69 -0.6847482225334041 1105 70-71 -0.8972855442189136 1105 72-73 0.06326916980637165 1105 74-75 -0.03507868536083336 1105 76-77 0.06443273960413265 1105 78-79 -0.14785970685471383 1105 80-81 0.1554998939590142 1105 82-83 0.468163863361422 1105 84-85 0.41227302301289015 1105 86-87 0.1386893408950236 1105 88-89 0.49237362002162754 1105 90-91 0.030813980472021996 1105 92-93 0.8444661375061742 1105 94-95 -0.01776319848368857 1105 96-97 0.43708521334334804 1105 98-99 -0.9603317308971349 1105 100-101 -0.8936294889394603 1106 1 0.20191488515213862 1106 2 0.20839576119841752 1106 3 0.08105847433252222 1106 4 -0.5750284678549065 1106 5 0.14502025067882585 1106 6 0.023886977675410037 1106 7 0.08605734318395974 1106 8 -0.11513503522505886 1106 9 -0.3560739454385242 1106 10-11 -0.16522024807940028 1106 12-13 0.062030698056240396 1106 14-15 0.24664487921629785 1106 16-17 -0.142295413302854 1106 18-19 -0.1458141030781661 1106 20-21 0.42607035236700597 1106 22-23 0.29168921285322824 1106 24-25 0.3655469502489481 1106 26-27 0.5185060336993246 1106 28-29 0.3506835358724061 1106 30-31 0.505770625117286 1106 32-33 0.7689336786807175 1106 34-35 0.06586780741465503 1106 36-37 0.7622345785073747 1106 38-39 0.6481983799735005 1106 40-41 1.495639779635347 1106 42-43 1.4076497305503821 1106 44-45 1.1477478525251925 1106 46-47 1.0153248786879487 1106 48-49 1.2413431274105378 1106 50-51 1.4052716606548472 1106 52-53 1.962564487545464 1106 54-55 2.077320093118722 1106 56-57 1.3838160337094223 1106 58-59 1.9350606590010742 1106 60-61 1.6550604282588353 1106 62-63 1.1982714638475045 1106 64-65 0.6438087451512544 1106 66-67 1.7125375005050607 1106 68-69 0.7037133159281339 1106 70-71 1.2623298403964718 1106 72-73 0.717115323652525 1106 74-75 0.9251439867039437 1106 76-77 1.3857890149077754 1106 78-79 1.8875653943598607 1106 80-81 1.355803537683709 1106 82-83 0.5912407864383447 1106 84-85 0.7275564238226018 1106 86-87 1.852656952231058 1106 88-89 1.6752683568637323 1106 90-91 0.8959961667068406 1106 92-93 1.5984135059272297 1106 94-95 0.46169024281185855 1106 96-97 -0.19236822795219766 1106 98-99 0.5172998480502358 1106 100-101 0.23896160417794832 1107 1 0.3645522477895007 1107 2 0.4861180389206936 1107 3 0.4746648679389196 1107 4 0.3332632404367999 1107 5 0.13203323769181452 1107 6 0.15855231234074552 1107 7 0.17596743309405127 1107 8 0.36718264709262627 1107 9 0.5078621184975418 1107 10-11 0.13477975192059688 1107 12-13 0.4773154133409534 1107 14-15 0.6526388852103011 1107 16-17 0.36329899229155416 1107 18-19 0.6616784044143387 1107 20-21 0.7164799427765942 1107 22-23 0.671209692373715 1107 24-25 0.3074050921070963 1107 26-27 0.7102143254076196 1107 28-29 1.1023318875207622 1107 30-31 0.6759004952471557 1107 32-33 0.3204821302291734 1107 34-35 0.494539136085983 1107 36-37 0.5376591539319548 1107 38-39 0.26827830005342435 1107 40-41 0.6737616577572254 1107 42-43 0.8796777025783555 1107 44-45 0.32027532505266976 1107 46-47 -0.0441156807526113 1107 48-49 -0.14606946000204601 1107 50-51 -0.4415814861983023 1107 52-53 -0.8112616862807087 1107 54-55 -0.16413844833981983 1107 56-57 0.04445539434878043 1107 58-59 -0.13387040992999744 1107 60-61 -0.2468376736392628 1107 62-63 -0.17325700768097363 1107 64-65 -0.25898845764594824 1107 66-67 -0.3018481138805562 1107 68-69 -0.8178651056502879 1107 70-71 -0.26903879097216077 1107 72-73 -0.31335420681700654 1107 74-75 0.22594318750314457 1107 76-77 -0.29552966641090705 1107 78-79 0.33112183791630656 1107 80-81 0.251108232988404 1107 82-83 -0.16819977300221467 1107 84-85 0.10118279744898118 1107 86-87 0.36134826092236594 1107 88-89 0.3921514737468499 1107 90-91 0.41188028259096043 1107 92-93 -0.38340467589095084 1107 94-95 -0.6236244425028268 1107 96-97 -0.6551054906894613 1107 98-99 -0.23075210000171253 1107 100-101 -1.2225768573605116 1108 1 0.1307860140232684 1108 2 0.13546868827134517 1108 3 0.3318077250817737 1108 4 0.4891073962809571 1108 5 -0.28355117789260476 1108 6 0.08063023441866335 1108 7 0.240902498029115 1108 8 -0.035414755504774575 1108 9 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64-65 1.388605125241753 2316 66-67 1.4084651023150165 2316 68-69 1.9064282480548336 2316 70-71 1.4012438675457908 2316 72-73 1.1867985815710789 2316 74-75 0.9283114075184251 2316 76-77 0.3473274764462353 2316 78-79 0.4043074758078262 2316 80-81 0.8114596462809978 2316 82-83 0.3858109221849517 2316 84-85 0.5759727134153643 2316 86-87 0.3775438300591105 2316 88-89 0.31056247451079244 2316 90-91 1.0905663024534498 2316 92-93 1.2866488000448761 2316 94-95 1.1015092473367432 2316 96-97 1.7678127675229156 2316 98-99 0.7114174951090568 2316 100-101 1.1303643191100754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 11.0 7 36.0 8 46.0 9 66.0 10 126.0 11 215.0 12 302.0 13 423.0 14 594.0 15 842.0 16 1110.0 17 1481.0 18 1348.0 19 870.0 20 739.0 21 744.0 22 752.0 23 751.0 24 826.0 25 858.0 26 928.0 27 1036.0 28 1316.0 29 1496.0 30 1979.0 31 2297.0 32 2882.0 33 3541.0 34 4889.0 35 7110.0 36 9520.0 37 14194.0 38 10299.0 39 637.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.913007177581164 24.584225481086467 17.45916319907351 18.04360414225885 2 14.754473602714457 25.458805154236625 39.067444021058584 20.719277221990332 3 18.00751322893805 29.40392357511209 33.75701840606445 18.831544789885417 4 10.95477251612382 21.96205684740605 40.18500316417348 26.898167472296652 5 9.310721069008714 42.233657071271736 35.851394857012004 12.604227002707544 6 24.364135776703606 42.78770415651214 17.416418694206197 15.431741372578061 7 21.948592279416715 38.93683771156202 23.22368686800684 15.89088314101442 8 23.509135709380764 35.302750811240216 21.326529238309387 19.861584241069625 9 24.256419232788918 19.067174729698795 24.121773552895558 32.55463248461673 10-11 21.76682061156068 30.178809462898382 28.139600640913436 19.914769284627504 12-13 24.319647738443102 29.3558030243863 28.63539043669122 17.689158800479376 14-15 21.616017450080115 31.05400638220523 28.979116455048544 18.35085971266612 16-17 16.308957977083306 33.79539242483405 30.74364809005103 19.152001508031617 18-19 15.706418559560516 31.492278070258116 35.767278406872315 17.03402496330905 20-21 17.240032853545895 29.986266140650876 35.580794140220014 17.19290686558322 22-23 17.223434049657996 29.582727419615445 35.84248936284806 17.351349167878492 24-25 17.96106046937484 30.178809462898382 35.567329572230676 16.292800495496103 26-27 16.89096845861245 29.66169589659003 37.15689904736257 16.290436597434947 28-29 16.283030935469757 30.3086814555492 37.22489648904298 16.18339111993806 30-31 16.94852495657677 29.727073206856158 36.496384763494866 16.82801707307221 32-33 16.18171780958408 31.315219001198347 36.38732176278124 16.115741426436333 34-35 17.254843878334164 30.304703173598675 36.179294187346 16.261158760721163 36-37 17.322166718280844 29.669848792901483 35.67975871494163 17.328225773876046 38-39 16.555359571288157 30.720085096069695 36.14495953897319 16.57959579366896 40-41 16.155570665894693 30.743855066414426 36.67234426439204 16.42823000329884 42-43 16.18671855537526 30.841687819394974 35.21946095890872 17.752132666321042 44-45 16.74655643673673 30.448100822685102 34.74935706687851 18.05598567369966 46-47 17.993375432549247 30.80423864600304 34.09767197619465 17.104713945253067 48-49 17.612328158451035 30.598230755766203 34.272711360056014 17.51672972572675 50-51 16.83609581386581 30.961100863078805 34.17307355693492 18.029729766120454 52-53 17.227241513956024 31.923144245916866 33.43858137311664 17.411032867010462 54-55 17.802178567100675 30.549085082605128 32.981459289878686 18.667277060415515 56-57 18.196017180788754 31.31185285920101 32.007971024249684 18.484158935760547 58-59 18.226985687164227 30.337018136773082 32.991557715870684 18.444438460192007 60-61 17.815643135090014 31.73329383726723 32.877108887961334 17.573954139681426 62-63 17.35515490985472 31.65519934292908 33.431849089121975 17.557796658094222 64-65 17.321493489881377 31.711750528484295 32.82930967159919 18.137446310035145 66-67 17.481048620555008 31.496317440654913 31.581144218987735 19.44148971980234 68-69 16.92092259219863 30.394915779127224 33.09254197579071 19.591619652883438 70-71 18.404717984623463 30.275081124022137 32.836041955593856 18.484158935760547 72-73 17.670899029204648 29.464514131064107 33.662766430139094 19.20182040959216 74-75 17.252824193135762 31.20480954368579 32.03018756143209 19.512178701746354 76-77 17.301296637897373 29.970108659063676 33.396167983950235 19.33242671908872 78-79 16.92900133299223 29.88460865233139 33.33153805760142 19.854851957074956 80-81 18.138792766834076 30.338364593572013 31.791864708020846 19.730977931573065 82-83 17.763370496041365 29.788064846232565 32.58940593526148 19.85915872246459 84-85 16.838115499064212 29.49750232263798 32.81651833200932 20.84786384628849 86-87 17.277733643916036 30.242092932448262 32.22407734047853 20.25609608315717 88-89 17.812276993092677 29.40055743311476 32.02682141943476 20.760344154357806 90-91 17.62400770271817 28.125694355604335 32.795130589352205 21.45516735232529 92-93 18.513357031292077 29.456158776323583 32.150455108525875 19.880029083858457 94-95 17.76245809153213 28.262128209616392 32.811132504813585 21.16428119403789 96-97 17.557796658094222 28.484966809839907 32.46374665068871 21.493489881377155 98-99 17.72040259871411 29.106271249200528 31.59390042750867 21.579425724576698 100-101 17.380881838015462 28.271204244889166 32.664906941040215 21.68300697605516 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 613.0 1 396.0 2 198.0 3 221.5 4 179.0 5 163.5 6 243.0 7 428.5 8 570.0 9 579.5 10 455.0 11 315.5 12 304.5 13 289.5 14 281.5 15 304.0 16 306.0 17 271.5 18 276.0 19 289.0 20 277.5 21 309.0 22 348.0 23 383.0 24 411.5 25 504.0 26 715.5 27 946.0 28 964.0 29 1028.0 30 1362.0 31 1639.5 32 2090.0 33 2661.5 34 3061.0 35 3533.0 36 4195.0 37 4645.5 38 4318.5 39 3578.5 40 3129.5 41 2761.0 42 2170.5 43 2074.5 44 2237.0 45 2071.5 46 1806.0 47 2068.0 48 3210.0 49 2946.5 50 1608.0 51 956.0 52 585.0 53 436.5 54 354.0 55 337.0 56 304.0 57 265.5 58 253.5 59 222.5 60 203.5 61 185.5 62 158.0 63 106.5 64 75.0 65 64.0 66 49.5 67 47.0 68 39.5 69 30.5 70 29.0 71 27.0 72 17.5 73 15.5 74 18.0 75 13.5 76 10.5 77 8.5 78 5.5 79 3.0 80 4.5 81 5.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013464567989336063 2 0.0 3 0.0 4 0.0 5 0.0430866175658754 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.008078740793601637 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013464567989336063 24-25 0.0 26-27 0.002019685198400409 28-29 0.002019685198400409 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.732283994668032E-4 42-43 0.010098425992002046 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0013464567989336063 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012791339589869258 92-93 0.0013464567989336063 94-95 0.0 96-97 0.0 98-99 0.002019685198400409 100-101 0.020196851984004092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74269.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.54023886143614 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89441991168205 24.44896255503642 2 9.843436370935366 6.603024141970406 3 4.27940586109996 4.305968842989673 4 2.7177840224809313 3.6462050115122056 5 1.6659975913287834 2.793897857787233 6 1.1842633480529907 2.383228534112483 7 0.895222802087515 2.1018190631353595 8 0.7788036932958651 2.0897009519449568 9 0.5861099959855479 1.7692442337987586 >10 4.757125652348455 30.718065410871294 >50 0.264953833801686 5.869205186551589 >100 0.11240465676435167 6.72824462427123 >500 0.008028904054596548 1.972559210437733 >1k 0.01204335608189482 4.5698743755806595 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 1.6130552451224602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1141 1.5363072075832447 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 1.4205119228749545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 1.114866229517026 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 637 0.8576929809207071 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.5910945347318531 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 383 0.5156929539915712 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.44433074364809005 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 272 0.36623624930994086 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 262 0.3527716813206048 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 208 0.28006301417819013 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 207 0.2787165573792565 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.24370868060698275 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.24236222380804914 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 178 0.23966931021018192 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 176 0.23697639661231468 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 176 0.23697639661231468 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 173 0.23293702621551388 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 155 0.208700803834709 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 143 0.1925433222475057 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 142 0.1911968654485721 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 133 0.17907875425816963 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 133 0.17907875425816963 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 132 0.17773229745923602 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 130 0.1750393838613688 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.16696064306776717 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 111 0.14945670468163028 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 111 0.14945670468163028 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 107 0.14407087748589587 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 105 0.14137796388802865 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 104 0.14003150708909506 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 101 0.13599213669229424 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 101 0.13599213669229424 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 98 0.1319527662954934 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.1306063094965598 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 96 0.1292598526976262 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 94 0.126566939099759 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 92 0.12387402550189178 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 91 0.12252756870295817 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 89 0.11983465510509096 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 84 0.11310237111042291 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 82 0.11040945751255571 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 81 0.10906300071362211 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 81 0.10906300071362211 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 79 0.1063700871157549 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 78 0.1050236303168213 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 77 0.10367717351788769 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 76 0.10233071671895407 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 75 0.10098425992002046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0013464567989336061 0.0 22-23 0.0 0.0 0.0 0.0013464567989336061 0.0 24-25 0.0 0.0 0.0 0.0026929135978672122 0.0 26-27 0.0 0.0 0.0 0.006732283994668031 0.0 28-29 0.0 0.0 0.0 0.026929135978672122 0.0 30-31 0.0 0.0 0.0 0.049145673161076625 0.0 32-33 0.0 0.0 0.0 0.09425197592535244 0.0 34-35 0.0 0.0 0.0 0.14407087748589587 0.0 36-37 0.0 0.0 0.0 0.1979291494432401 0.0 38-39 0.0 0.0 0.0 0.27737010058032285 0.0 40-41 0.0 0.0 0.0 0.34738585412487044 0.0 42-43 0.0 0.0 0.0 0.4174016076694179 0.0 44-45 0.0 0.0 0.0 0.46318113883316053 0.0 46-47 0.0 0.0 0.0 0.5338701207771748 0.0 48-49 0.0 0.0 0.0 0.5843622507371851 0.0 50-51 0.0 0.0 0.0 0.6577441462790666 0.0 52-53 0.0 0.0 0.0 0.738531554215083 0.0 54-55 0.0 0.0 0.0 0.8523071537249727 0.0 56-57 0.0 0.0 0.0 1.0212874819911404 0.0 58-59 0.0 0.0 0.0 1.2077717486434447 0.0 60-61 0.0 0.0 0.0 1.4009882992904172 0.0 62-63 0.0 0.0 0.0 1.5605434299640497 0.0 64-65 0.0 0.0 0.0 1.7901143141822295 0.0 66-67 0.0 0.0 0.0 2.080948982751888 0.0 68-69 0.0 0.0 0.0 2.4088112132922213 0.0 70-71 0.0 0.0 0.0 2.748791555022957 0.0 72-73 0.0 0.0 0.0 3.059149847177153 0.0 74-75 0.0 0.0 0.0 3.411921528497758 0.0 76-77 0.0 0.0 0.0 3.815185339778373 0.0 78-79 0.0 0.0 0.0 4.232586947447791 0.0 80-81 0.0 0.0 0.0 4.6223861907390695 0.0 82-83 0.0 0.0 0.0 5.010165748831948 0.0 84-85 0.0 0.0 0.0 5.551441382003258 0.0 86-87 0.0 0.0 0.0 6.163405997118582 0.0 88-89 0.0 0.0 0.0 6.817784001400315 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACTA 15 6.59336E-4 95.0 2 ACGCAGC 20 0.0020664204 71.25 8 TCACTAC 20 0.0020664204 71.25 3 CGTATAG 45 9.5669244E-4 42.222225 1 CTATTCC 50 0.0016060974 38.000004 4 TAACAGT 70 2.0349558E-4 33.928574 4 TACTAAC 70 2.0349558E-4 33.928574 1 AACAGTG 70 2.0349558E-4 33.928574 5 GTATAGG 75 3.0509988E-4 31.666668 1 ACAGTGT 80 4.4537196E-4 29.687502 6 CTAACAG 80 4.4537196E-4 29.687502 3 GAACTAA 65 0.0058093923 29.23077 1 ACTAGAA 70 0.008341996 27.142859 7 GGAAAAG 70 0.008341996 27.142859 7 GTGTTGC 90 8.8686415E-4 26.388891 9 CTATTAT 150 1.0379335E-6 25.333334 1 TAGGACA 95 0.0012157782 25.000002 4 GAGTACT 865 0.0 24.436417 12-13 GTACTTT 865 0.0 24.16185 14-15 TTCTAGG 100 0.0016391703 23.750002 1 >>END_MODULE