##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76465 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60015693454522 33.0 31.0 34.0 30.0 34.0 2 31.701340482573727 33.0 31.0 34.0 30.0 34.0 3 31.707630942261165 33.0 31.0 34.0 30.0 34.0 4 35.324318315569215 37.0 35.0 37.0 33.0 37.0 5 35.32150657163408 37.0 35.0 37.0 33.0 37.0 6 35.438867455698684 37.0 35.0 37.0 33.0 37.0 7 35.397253645458704 37.0 35.0 37.0 33.0 37.0 8 35.393801085463934 37.0 35.0 37.0 33.0 37.0 9 36.982384097299416 39.0 37.0 39.0 33.0 39.0 10-11 36.90736938468581 39.0 37.0 39.0 33.0 39.0 12-13 36.80629699862682 39.0 37.0 39.0 33.0 39.0 14-15 37.853514679918916 40.0 38.0 41.0 33.0 41.0 16-17 37.82021840057543 40.0 38.0 41.0 33.0 41.0 18-19 37.860027463545414 40.0 38.0 41.0 33.0 41.0 20-21 37.74779964689728 40.0 37.5 41.0 33.0 41.0 22-23 37.62408945269077 40.0 37.0 41.0 33.0 41.0 24-25 37.45926894657687 40.0 37.0 41.0 32.5 41.0 26-27 37.470659778983844 40.0 37.0 41.0 33.0 41.0 28-29 37.373399594585756 40.0 36.5 41.0 33.0 41.0 30-31 37.126070751324136 39.0 36.0 41.0 32.5 41.0 32-33 36.775374354279734 39.0 35.0 41.0 32.0 41.0 34-35 36.46355849081279 39.0 35.0 40.5 31.0 41.0 36-37 36.324978748447 39.0 35.0 41.0 31.0 41.0 38-39 36.19719479500425 39.0 35.0 41.0 30.5 41.0 40-41 35.983502255934084 39.0 35.0 41.0 30.0 41.0 42-43 35.63946249918263 39.0 35.0 40.0 29.5 41.0 44-45 35.26901196625907 38.0 35.0 40.0 27.5 41.0 46-47 34.710455764075064 38.0 35.0 40.0 23.5 41.0 48-49 34.249970574772775 38.0 35.0 40.0 21.5 41.0 50-51 33.28550971032499 37.5 33.5 39.5 16.5 40.5 52-53 33.38841953835088 38.0 33.5 39.5 14.5 40.5 54-55 33.67143137383117 38.0 34.0 40.0 10.0 41.0 56-57 33.333172039495196 38.0 33.5 40.0 4.5 41.0 58-59 33.10844830968417 38.0 33.0 40.0 2.0 41.0 60-61 32.77642712352056 38.0 33.0 40.0 2.0 41.0 62-63 32.39157784607337 37.0 33.0 40.0 2.0 41.0 64-65 32.00102661348329 36.5 32.0 39.5 2.0 41.0 66-67 31.547950042503107 36.0 31.5 39.0 2.0 41.0 68-69 31.023010527692406 36.0 31.0 39.0 2.0 40.0 70-71 30.525076832537763 35.0 30.5 38.5 2.0 40.0 72-73 29.999816909697245 35.0 29.5 37.5 2.0 40.0 74-75 29.476832537762377 35.0 29.0 37.0 2.0 39.0 76-77 29.024606028902113 34.5 29.0 36.5 2.0 39.0 78-79 28.465650951415682 34.0 28.0 36.0 2.0 38.5 80-81 27.894611913947557 34.0 26.5 35.5 2.0 37.5 82-83 27.35669914339894 34.0 26.0 35.0 2.0 37.0 84-85 26.833126266919507 33.5 25.0 35.0 2.0 36.0 86-87 26.365036291113583 33.0 24.5 35.0 2.0 36.0 88-89 25.841299941149547 33.0 21.5 35.0 2.0 35.5 90-91 25.331177662983066 32.5 19.0 35.0 2.0 35.0 92-93 24.818851762244165 32.0 14.5 35.0 2.0 35.0 94-95 24.204269927417773 32.0 4.5 34.5 2.0 35.0 96-97 23.439482116000782 31.0 2.0 34.0 2.0 35.0 98-99 22.620976917543974 31.0 2.0 34.0 2.0 35.0 100-101 20.29038775910547 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5263034208092847 1101 2 0.41884923311510747 1101 3 0.5246909854478155 1101 4 0.19575095466833403 1101 5 0.34140703642046333 1101 6 -0.16580659396542075 1101 7 0.49823415076052413 1101 8 0.7332519463932314 1101 9 -0.1561638875023874 1101 10-11 -0.34416815201982587 1101 12-13 0.27504407048097335 1101 14-15 0.2013756285584165 1101 16-17 0.5045135420940099 1101 18-19 0.6451148531344018 1101 20-21 0.549099061380204 1101 22-23 -0.05043437854032362 1101 24-25 -0.027837095600254713 1101 26-27 -0.37191834322791806 1101 28-29 0.09533116384056939 1101 30-31 -0.5190917455557482 1101 32-33 -0.377628762911705 1101 34-35 0.26729474124918795 1101 36-37 0.23877410236128327 1101 38-39 0.706694805365963 1101 40-41 0.4650257818858208 1101 42-43 0.5716340632076253 1101 44-45 0.7981154278916804 1101 46-47 1.7998012451454457 1101 48-49 1.374851077287758 1101 50-51 1.7038221610276452 1101 52-53 1.6548402378421727 1101 54-55 0.7671067805773362 1101 56-57 1.2541870982223529 1101 58-59 1.2325171098440109 1101 60-61 1.2205940617315676 1101 62-63 0.9128995517246068 1101 64-65 1.0370693589221958 1101 66-67 1.4006395516310448 1101 68-69 1.1364618695539654 1101 70-71 0.9139045478665331 1101 72-73 0.9377326375276525 1101 74-75 0.604535057288512 1101 76-77 -0.03740273788439552 1101 78-79 0.6244703834926284 1101 80-81 0.8798806071659371 1101 82-83 1.4714210776863226 1101 84-85 1.3461040923007204 1101 86-87 0.8371481051946894 1101 88-89 0.61595956047233 1101 90-91 0.39557728154521143 1101 92-93 1.0074372792188733 1101 94-95 1.7450620887824009 1101 96-97 2.73932806814409 1101 98-99 2.481271615703733 1101 100-101 1.7684697762702477 1102 1 -0.07994657919071813 1102 2 -0.1731150525991758 1102 3 -0.03959472883790127 1102 4 0.1453045260969077 1102 5 -0.15680724929381995 1102 6 -0.3836637368225624 1102 7 0.32100200790338107 1102 8 -0.5944266250353394 1102 9 -0.3244674589309611 1102 10-11 0.09623363369446025 1102 12-13 -0.14772378666188501 1102 14-15 -0.021615442870150048 1102 16-17 0.43777247066544334 1102 18-19 0.21096306742011706 1102 20-21 0.4569115613802026 1102 22-23 0.7466638357453874 1102 24-25 0.8978325472568898 1102 26-27 0.12049237105779298 1102 28-29 0.2489025924119943 1102 30-31 0.3170689687299628 1102 32-33 0.9748265942311534 1102 34-35 1.473544741249185 1102 36-37 1.2811848166469915 1102 38-39 1.0352662339373921 1102 40-41 0.9994007818858179 1102 42-43 1.3591340632076268 1102 44-45 1.5150797136059637 1102 46-47 0.6176583880025888 1102 48-49 1.364360005859183 1102 50-51 1.6422150181705035 1102 52-53 2.231402737842167 1102 54-55 2.2371960662916237 1102 56-57 1.8236067410794945 1102 58-59 2.2425617527011568 1102 60-61 2.616799418874429 1102 62-63 1.4486138374388915 1102 64-65 1.7417568589222014 1102 66-67 1.8071127659167558 1102 68-69 1.5087832981253904 1102 70-71 1.5194849050093886 1102 72-73 0.6404112089562233 1102 74-75 -0.04658101414005955 1102 76-77 0.6407222621156059 1102 78-79 0.5157650263497686 1102 80-81 0.6734073928802218 1102 82-83 0.2089210776863233 1102 84-85 0.16083623515786272 1102 86-87 0.45321953376612 1102 88-89 0.8376113461866161 1102 90-91 0.6382112101166406 1102 92-93 0.3257408506474455 1102 94-95 0.2620263744966884 1102 96-97 0.49133699671551767 1102 98-99 1.2410930442751607 1102 100-101 1.7202554905559602 1103 1 0.47495206945792745 1103 2 0.3277295419954207 1103 3 0.3725674333242601 1103 4 0.2563687152860936 1103 5 0.2773143723278011 1103 6 0.43728220912338145 1103 7 -0.06624461371823998 1103 8 0.1429044560457413 1103 9 0.4588940275555231 1103 10-11 0.36587045801878304 1103 12-13 0.35438769982459917 1103 14-15 0.769136246319043 1103 16-17 0.7884903760708468 1103 18-19 0.7512924593120047 1103 20-21 0.922072034353171 1103 22-23 1.0530405249345804 1103 24-25 1.1028578850947284 1103 26-27 1.0896646683550912 1103 28-29 1.144172862682268 1103 30-31 1.0573561308921242 1103 32-33 0.9484330131500727 1103 34-35 1.5335109574654027 1103 36-37 1.2571138707010405 1103 38-39 0.8190500177211746 1103 40-41 1.5420528089128425 1103 42-43 1.5837962253697881 1103 44-45 2.0040999838762374 1103 46-47 1.0331989285431291 1103 48-49 1.3763954788321513 1103 50-51 1.0636677208732053 1103 52-53 1.2480834810854091 1103 54-55 1.610929174399736 1103 56-57 1.8673145113497682 1103 58-59 2.1929418202687216 1103 60-61 2.4223315134690253 1103 62-63 2.0026678914929406 1103 64-65 2.646528818381661 1103 66-67 2.760871211862707 1103 68-69 2.698817081909173 1103 70-71 2.4318582158201956 1103 72-73 2.9807828305778443 1103 74-75 2.984457837211295 1103 76-77 3.2652999648183147 1103 78-79 3.0700302290524704 1103 80-81 3.5169462442315726 1103 82-83 3.3119616182268636 1103 84-85 3.5835558297524592 1103 86-87 3.9076114256580112 1103 88-89 4.0422143867271565 1103 90-91 3.0552297911977213 1103 92-93 3.7315685533501473 1103 94-95 4.4132088069291235 1103 96-97 3.238169766985788 1103 98-99 3.2976808821129993 1103 100-101 3.9246473824478514 1104 1 0.134522598891472 1104 2 0.3564226186336974 1104 3 0.25150116157305646 1104 4 0.3343028137681401 1104 5 -0.13882779723902416 1104 6 -0.01551305189105534 1104 7 -0.006266827713055534 1104 8 0.2193576215400057 1104 9 0.3833664451786305 1104 10-11 0.4114091473930941 1104 12-13 0.4261203914203122 1104 14-15 0.4772073311024556 1104 16-17 0.9413041487476406 1104 18-19 0.6915923482420325 1104 20-21 0.637455225763766 1104 22-23 0.6583718836905916 1104 24-25 1.1520063486267489 1104 26-27 0.8711344943454691 1104 28-29 0.7060943732339098 1104 30-31 0.6828652016066741 1104 32-33 0.49869217642292796 1104 34-35 0.7248289878245231 1104 36-37 0.26470365226342807 1104 38-39 0.3195128092798569 1104 40-41 -0.491823533182675 1104 42-43 -0.619461827203331 1104 44-45 0.03519957661966089 1104 46-47 -0.5176155846001507 1104 48-49 -0.9493172201682114 1104 50-51 -1.2588979955281303 1104 52-53 -2.145844693664685 1104 54-55 -2.486904961105637 1104 56-57 -2.539942060290368 1104 58-59 -1.6383243774358291 1104 60-61 -2.2167836290707754 1104 62-63 -2.7363176694104254 1104 64-65 -2.3643005040914957 1104 66-67 -2.5686363779188603 1104 68-69 -2.023322866258173 1104 70-71 -1.9029251977303367 1104 72-73 -1.524400434879393 1104 74-75 -1.3223729661948553 1104 76-77 -1.9065808200761758 1104 78-79 -1.4271930215954356 1104 80-81 -1.0227612715033416 1104 82-83 -0.14981179902600772 1104 84-85 -0.18912095662296124 1104 86-87 -0.10756813746675675 1104 88-89 0.13181083248798586 1104 90-91 0.40565555943171105 1104 92-93 0.2959891383186779 1104 94-95 0.4020092512090194 1104 96-97 0.04324196246894374 1104 98-99 0.8125827703025585 1104 100-101 1.1971390521997982 1105 1 0.5977319922378541 1105 2 0.8045635188293936 1105 3 0.5675481283049528 1105 4 0.5957509546683397 1105 5 0.5271213221347537 1105 6 0.8341934060345793 1105 7 0.3696627221890978 1105 8 0.5618233749646606 1105 9 0.8724075410690375 1105 10-11 0.4272604194087464 1105 12-13 0.6464726419095399 1105 14-15 0.9585184857012834 1105 16-17 1.0759421135225864 1105 18-19 1.1879719959915462 1105 20-21 1.0276704899516318 1105 22-23 1.1067084786025276 1105 24-25 0.8793057615426036 1105 26-27 1.1137959424863624 1105 28-29 0.5810454495548498 1105 30-31 1.3809082544442433 1105 32-33 1.9652283799454366 1105 34-35 1.2601518841063282 1105 36-37 1.5673455309327053 1105 38-39 1.5352662339373921 1105 40-41 1.8650257818858194 1105 42-43 2.443062634636199 1105 44-45 1.8266868564631054 1105 46-47 1.5569441022883055 1105 48-49 1.6034225058591858 1105 50-51 0.8252507324562117 1105 52-53 0.811983094985024 1105 54-55 1.074249637720193 1105 56-57 1.497044241079493 1105 58-59 1.2896599669868678 1105 60-61 1.2848797760172843 1105 62-63 1.7557566945817484 1105 64-65 1.6370693589222043 1105 66-67 2.1292109802024726 1105 68-69 1.4436047266968153 1105 70-71 1.6567616907236733 1105 72-73 1.8234469232419386 1105 74-75 2.011677914431367 1105 76-77 2.3840258335441753 1105 78-79 2.6173275263497686 1105 80-81 2.5941663214516524 1105 82-83 1.9071353634006094 1105 84-85 0.9318183780150058 1105 86-87 2.1228623909089777 1105 88-89 1.687388131900903 1105 90-91 1.5884344244023545 1105 92-93 2.014580136361733 1105 94-95 1.7093478030681162 1105 96-97 1.2036137824298052 1105 98-99 1.981271615703733 1105 100-101 2.9970412048416755 1106 1 -0.16665432566959026 1106 2 0.4303180459923759 1106 3 0.5343489331339306 1106 4 0.2995738922940916 1106 5 0.05005894788927634 1106 6 -0.02979049738594597 1106 7 -0.19110186533605855 1106 8 0.09703464257029282 1106 9 0.028745569238054713 1106 10-11 -0.268313021235123 1106 12-13 -0.022943857084420927 1106 14-15 0.05882029656647347 1106 16-17 -0.12043615609511704 1106 18-19 -0.524402249481291 1106 20-21 -0.7163938963662773 1106 22-23 -0.5023458473532045 1106 24-25 0.0995270895104099 1106 26-27 -0.2604495303506553 1106 28-29 0.09573357832748286 1106 30-31 0.7895601659130662 1106 32-33 0.24430282662552116 1106 34-35 -0.10171934325785514 1106 36-37 -0.27581342278963206 1106 38-39 -0.39783235761190383 1106 40-41 -0.01314323219869351 1106 42-43 -0.726253260736037 1106 44-45 -0.2943393004785477 1106 46-47 -0.41227118946219576 1106 48-49 -1.071929606816873 1106 50-51 -0.8045279395759763 1106 52-53 -0.4388217339888172 1106 54-55 -0.6556296379337283 1106 56-57 0.09000198755836664 1106 58-59 -0.3429356064537714 1106 60-61 -0.9363475881678234 1106 62-63 0.20213496419945187 1106 64-65 -0.14743768333131868 1106 66-67 -0.12873670591422837 1106 68-69 0.11221639671694206 1106 70-71 -0.6782483696384993 1106 72-73 -0.16900780512828462 1106 74-75 -0.07463999301329949 1106 76-77 0.5449916283127898 1106 78-79 0.3462007657863886 1106 80-81 0.5775667238661377 1106 82-83 0.45804079599617964 1106 84-85 0.6086795450170186 1106 86-87 -0.14625229721979593 1106 88-89 -0.20959375944718772 1106 90-91 0.636120541102553 1106 92-93 -0.020429924000442412 1106 94-95 -0.5861250339540156 1106 96-97 -1.2433681089182862 1106 98-99 -1.3183259698093437 1106 100-101 -0.8616107066271361 1107 1 -0.5594108649050042 1107 2 0.2617063759722562 1107 3 0.5389766997335279 1107 4 0.5528938118111952 1107 5 0.4985498935633217 1107 6 0.11990769174886395 1107 7 0.16966272218909495 1107 8 -0.2953194821781935 1107 9 0.10097896964046527 1107 10-11 -0.1870252948769675 1107 12-13 0.2607583561952538 1107 14-15 0.05851848570127771 1107 16-17 -0.059772172191699724 1107 18-19 0.18082913884868645 1107 20-21 0.5133847756659193 1107 22-23 0.6209941928882472 1107 24-25 0.4364486186854606 1107 26-27 0.456653085343504 1107 28-29 0.438188306697711 1107 30-31 0.7809082544442489 1107 32-33 0.872371237088295 1107 34-35 0.84586616982061 1107 36-37 1.3387741023612776 1107 38-39 0.9352662339373978 1107 40-41 0.6578829247429567 1107 42-43 0.8430626346361976 1107 44-45 1.0266868564631082 1107 46-47 1.6712298165740194 1107 48-49 1.2105653630020399 1107 50-51 1.596679303884791 1107 52-53 1.919125952127878 1107 54-55 2.109963923434485 1107 56-57 1.9256156696509237 1107 58-59 2.1039456812725845 1107 60-61 2.1348797760172857 1107 62-63 2.512899551724608 1107 64-65 2.0727836446364876 1107 66-67 2.372068123059613 1107 68-69 2.307890440982529 1107 70-71 2.106761690723669 1107 72-73 2.2448754946705094 1107 74-75 2.1188207715742244 1107 76-77 0.7768829764013212 1107 78-79 1.2244703834926227 1107 80-81 1.6798806071659342 1107 82-83 1.7642782205434635 1107 84-85 1.6675326637292933 1107 86-87 1.6942909623375506 1107 88-89 1.4659595604723314 1107 90-91 1.002720138688069 1107 92-93 2.157437279218872 1107 94-95 2.6736335173538315 1107 96-97 2.532185211001231 1107 98-99 2.2027001871323044 1107 100-101 1.7184697762702434 1108 1 0.3529700874759456 1108 2 0.5531349474008245 1108 3 0.6104052711620973 1108 4 0.6071795260969068 1108 5 0.6023594173728455 1108 6 0.504669596510773 1108 7 0.5020436745700465 1108 8 0.508490041631326 1108 9 0.5857408744023758 1108 10-11 0.6482128003611294 1108 12-13 0.8179012133381178 1108 14-15 0.8066137237965165 1108 16-17 0.6730849706654425 1108 18-19 0.24273390075344992 1108 20-21 0.5857657280468729 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-0.8091430618855284 2316 80-81 -1.3902874600609536 2316 82-83 -1.1432848046666173 2316 84-85 -1.4816270001362533 2316 86-87 -1.131339289763293 2316 88-89 -0.9218555655780882 2316 90-91 -1.1670277604715942 2316 92-93 -1.2770165022937299 2316 94-95 -1.3927530372680188 2316 96-97 -1.2379828562256598 2316 98-99 -1.2960393086660176 2316 100-101 -1.4966562741499239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 598.0 3 86.0 4 75.0 5 76.0 6 91.0 7 84.0 8 95.0 9 140.0 10 157.0 11 184.0 12 309.0 13 477.0 14 565.0 15 821.0 16 1060.0 17 1422.0 18 1145.0 19 681.0 20 721.0 21 782.0 22 773.0 23 876.0 24 924.0 25 956.0 26 1057.0 27 1168.0 28 1358.0 29 1557.0 30 1921.0 31 2337.0 32 2860.0 33 3731.0 34 5064.0 35 7497.0 36 9321.0 37 13452.0 38 10893.0 39 1151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.68684058579602 24.17720854548318 16.512256574458036 18.62369429426277 2 14.37078622209397 25.168551116241243 39.17993651459902 21.280726147065767 3 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32.343690618160856 32.09273068001625 17.493152659651145 48-49 17.362440034602983 32.13489920570425 32.369517917529556 18.13314284216321 50-51 16.56902449573389 32.349047825004256 32.646561553886684 18.435366125375168 52-53 17.082027818927884 33.4344970437473 31.761035147288247 17.722439990036577 54-55 17.638848845438105 31.992241763426204 31.447723639032315 18.921185752103373 56-57 18.34108628709952 32.43661141322151 30.662386162615473 18.559916137063485 58-59 18.045600471729017 31.288082290506452 31.7119832274127 18.954334010351833 60-61 17.75723588527404 32.387547332975196 31.808414459978252 18.04680232177251 62-63 17.27770643884798 32.41371629235567 32.41568175266647 17.89289551612988 64-65 17.59738473028393 32.16742443102161 31.994470722343788 18.240720116350676 66-67 17.39771349952501 32.0712811609395 31.0315458446621 19.499459494873392 68-69 16.752273382426164 31.520087004376425 32.30234020807673 19.42529940512068 70-71 18.261689958134326 31.318670519095072 31.764189450373774 18.655450072396825 72-73 17.713151377769627 30.196936542669583 32.93478688137947 19.15512519818132 74-75 17.243593523742128 31.98880873285764 31.459385790759995 19.308211952640235 76-77 17.215896434701712 30.886804072380404 32.34581165895779 19.55148783396009 78-79 17.003655995701912 30.849920721240153 32.59143265236592 19.554990630692018 80-81 18.480760536988385 30.97904068034253 31.138905450471405 19.40129333219768 82-83 18.08647919245911 30.742625817988877 31.79659507012269 19.374299919429326 84-85 17.21987488126822 30.318037404605157 31.902001244636597 20.560086469490027 86-87 17.64917820154206 31.046229029236244 31.559157042442664 19.745435726779036 88-89 18.21951459172644 30.312796829452033 30.84340506370574 20.624283515115785 90-91 17.985809469525737 29.05848516414762 32.01648355248072 20.939221813845922 92-93 19.118745332337568 30.40957509531858 31.390930650000655 19.0807489223432 94-95 18.644323177003148 29.22035808106284 32.13296035952229 20.002358382411707 96-97 18.285092834025605 29.381936346126132 31.512467406543585 20.82050341330468 98-99 18.718511481639204 29.937432436859172 30.53428112818161 20.809774953320012 100-101 18.84846936435876 28.439098847683248 31.817437389042837 20.89499439891516 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 677.0 1 417.5 2 174.0 3 200.5 4 182.0 5 171.5 6 260.0 7 440.5 8 578.0 9 571.5 10 442.5 11 341.5 12 329.5 13 316.5 14 310.0 15 282.5 16 256.5 17 263.5 18 252.5 19 253.0 20 286.5 21 316.0 22 340.5 23 379.5 24 418.0 25 504.5 26 687.0 27 961.5 28 1060.5 29 1084.5 30 1418.5 31 1788.5 32 2267.5 33 2878.5 34 3261.5 35 3610.5 36 4206.0 37 4733.0 38 4455.5 39 3690.0 40 3178.5 41 2735.0 42 2205.5 43 2108.0 44 2177.0 45 2097.0 46 1873.5 47 2070.0 48 3204.5 49 3024.5 50 1693.5 51 946.5 52 574.0 53 453.0 54 404.5 55 356.0 56 331.5 57 309.0 58 278.5 59 253.5 60 235.0 61 226.0 62 178.0 63 130.5 64 102.0 65 84.5 66 72.5 67 68.5 68 63.0 69 50.0 70 38.0 71 40.0 72 38.5 73 31.0 74 25.5 75 16.5 76 16.5 77 17.0 78 11.5 79 7.5 80 3.0 81 0.5 82 0.0 83 1.0 84 1.5 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3413326358464657 2 0.2968678480350487 3 0.29294448440462956 4 0.2981756359118551 5 0.3073301510494998 6 0.2262473026875041 7 0.21447721179624668 8 0.21970836330347218 9 0.22755509056431047 10-11 0.2249395148106977 12-13 0.22428562087229453 14-15 0.2262473026875041 16-17 0.20532269665860195 18-19 0.21513110573464986 20-21 0.20597659059700515 22-23 0.2118616360426339 24-25 0.21120774210423068 26-27 0.21382331785784345 28-29 0.22363172693389133 30-31 0.22297783299548815 32-33 0.22886287844111683 34-35 0.22232393905708492 36-37 0.2033610148433924 38-39 0.1981298633361669 40-41 0.19616818152095727 42-43 0.2066304845354084 44-45 0.202053226966586 46-47 0.2066304845354084 48-49 0.22363172693389133 50-51 0.21709278754985942 52-53 0.24324854508598706 54-55 0.20793827241221474 56-57 0.19616818152095727 58-59 0.19616818152095727 60-61 0.188321454260119 62-63 0.19224481789053816 64-65 0.188321454260119 66-67 0.19289871182894136 68-69 0.19224481789053816 70-71 0.1955142875825541 72-73 0.19093703001373177 74-75 0.2033610148433924 76-77 0.19093703001373177 78-79 0.1987837572745701 80-81 0.19682207545936048 82-83 0.1758974694304584 84-85 0.17982083306087754 86-87 0.1811286209376839 88-89 0.17982083306087754 90-91 0.19159092395213498 92-93 0.18570587850650624 94-95 0.18505198456810307 96-97 0.19093703001373177 98-99 0.19289871182894136 100-101 0.18374419669129669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 76465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.682992218662136 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56477918270112 26.606944353625845 2 9.437419827744181 6.735107565552867 3 4.079164376030786 4.36670372065651 4 2.3382811068352574 3.337474661609887 5 1.594282572842221 2.844438632053881 6 1.081180135605644 2.314784541947296 7 0.868609125893348 2.1696200876217877 8 0.6963533076782115 1.9878375727457007 9 0.6743632032252154 2.1656967239913687 >10 4.262415246472421 29.01850519845681 >50 0.27121128825361923 6.341463414634147 >100 0.11361553967381345 6.508860262865364 >500 0.014660069635330768 4.020139933302818 >1k 0.003665017408832692 1.5824233309357223 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1210 1.5824233309357223 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 997 1.303864513175963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 861 1.1260053619302948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.8055973321127313 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 600 0.7846727260838291 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 450 0.5885045445628719 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.38972078728830184 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 281 0.36748839338259337 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.2759432420061466 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 197 0.25763421173085727 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 190 0.2484796965932126 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 189 0.24717190871640618 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.24455633296279344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 182 0.2380173935787615 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 176 0.2301706663179232 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 167 0.2184005754266658 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 161 0.2105538481658275 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.20139933302818283 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 151 0.19747596939776366 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 149 0.19486039364415092 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 143 0.18701366638331263 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 134 0.1752435754920552 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 120 0.15693454521676584 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 119 0.15562675733995945 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 119 0.15562675733995945 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 118 0.15431896946315307 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 115 0.15039560583273393 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 113 0.14778003007912116 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 111 0.14516445432550842 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 111 0.14516445432550842 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 110 0.14385666644870201 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 107 0.13993330281828287 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 106 0.13862551494147649 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 104 0.13600993918786372 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 103 0.13470215131105734 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 101 0.13208657555744457 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.12816321192702543 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 97 0.12685542405021905 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 91 0.11900869678938075 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 90 0.11770090891257438 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 89 0.116393121035768 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 86 0.11246975740534886 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.10985418165173609 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 83 0.10854639377492971 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 82 0.10723860589812334 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 82 0.10723860589812334 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 81 0.10593081802131694 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 81 0.10593081802131694 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 81 0.10593081802131694 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 80 0.10462303014451056 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 79 0.1033152422677042 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.001307787876806382 0.0 22-23 0.0 0.0 0.0 0.003923363630419146 0.0 24-25 0.0 0.0 0.0 0.009154515137644674 0.0 26-27 0.0 0.0 0.0 0.015039560583273392 0.0 28-29 0.0 0.0 0.0 0.03465637873536912 0.0 30-31 0.0 0.0 0.0 0.06735107565552867 0.0 32-33 0.0 0.0 0.0 0.1020074543908978 0.0 34-35 0.0 0.0 0.0 0.1458183482639116 0.0 36-37 0.0 0.0 0.0 0.20009154515137645 0.0 38-39 0.0 0.0 0.0 0.29294448440462956 0.0 40-41 0.0 0.0 0.0 0.3596416661217551 0.0 42-43 0.0 0.0 0.0 0.421107696331655 0.0 44-45 0.0 0.0 0.0 0.4596874386974433 0.0 46-47 0.0 0.0 0.0 0.5185378931537306 0.0 48-49 0.0 0.0 0.0 0.5741188779180018 0.0 50-51 0.0 0.0 0.0 0.6198914536062251 0.0 52-53 0.0 0.0 0.0 0.6748185444320931 0.0 54-55 0.0 0.0 0.0 0.7970967109134899 0.0 56-57 0.0 0.0 0.0 0.9455306349310142 0.0 58-59 0.0 0.0 0.0 1.1560844830968415 0.0 60-61 0.0 0.0 0.0 1.335251422219316 0.0 62-63 0.0 0.0 0.0 1.506571634080952 0.0 64-65 0.0 0.0 0.0 1.7053553913555222 0.0 66-67 0.0 0.0 0.0 1.9773752697312497 0.0 68-69 0.0 0.0 0.0 2.296475511672007 0.0 70-71 0.0 0.0 0.0 2.6606944353625845 0.0 72-73 0.0 0.0 0.0 3.009219904531485 0.0 74-75 0.0 0.0 0.0 3.4120185705878505 0.0 76-77 0.0 0.0 0.0 3.7729680245864117 0.0 78-79 0.0 0.0 0.0 4.152880402798666 0.0 80-81 0.0 0.0 0.0 4.534100568887727 0.0 82-83 0.0 0.0 0.0 4.923167462237625 0.0 84-85 0.0 0.0 0.0 5.458706597789838 0.0 86-87 0.0 0.0 0.0 6.075982475642451 0.0 88-89 0.0 0.0 0.0 6.73772314130648 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 25 5.2256066E-5 76.053444 5 AGGTAAG 20 0.0020608918 71.300095 2 TGAAGCA 30 1.2890021E-4 63.377865 4 GCAGATC 30 1.2931894E-4 63.33639 8 GGTAAGG 25 0.0049894857 57.04008 3 GTAAGGT 25 0.0049894857 57.04008 4 GGTGTGC 25 0.0050024437 57.00275 8 GTGTGCA 25 0.0050024437 57.00275 9 TCAGTTC 25 0.0050024437 57.00275 8 AGCAGAT 35 2.7708002E-4 54.288334 7 CAGATCA 45 9.566402E-4 42.22426 9 AAGCAGA 55 0.0025640372 34.54712 6 CGCAGAA 55 0.0025640372 34.54712 9 CTTTATG 55 0.0025640372 34.54712 9 CATGGGG 135 1.15851435E-8 31.688932 4 CATAGCT 60 0.0039145984 31.688932 4 TAGCTTT 60 0.00392718 31.668196 6 TGCACTT 30 0.003939402 31.668196 12-13 AGGCTAT 75 3.0509598E-4 31.668192 6 AATTATC 30 0.0039520464 31.647482 58-59 >>END_MODULE