##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937666_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76465 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93117112404368 31.0 31.0 34.0 30.0 34.0 2 32.130111815863465 33.0 31.0 34.0 30.0 34.0 3 32.17451121428105 34.0 31.0 34.0 30.0 34.0 4 35.91573922709736 37.0 35.0 37.0 35.0 37.0 5 35.8053226966586 37.0 35.0 37.0 35.0 37.0 6 35.874269273523836 37.0 35.0 37.0 35.0 37.0 7 35.814961093310664 37.0 35.0 37.0 35.0 37.0 8 35.814346433008566 37.0 35.0 37.0 35.0 37.0 9 37.39506963970444 39.0 37.0 39.0 34.0 39.0 10-11 37.3573334205192 39.0 37.0 39.0 34.0 39.0 12-13 37.27846727260838 39.0 37.0 39.0 33.5 39.0 14-15 38.28282874517753 40.0 38.0 41.0 33.0 41.0 16-17 38.2441443797816 40.0 37.5 41.0 33.5 41.0 18-19 38.36726606944353 40.0 38.0 41.0 34.0 41.0 20-21 38.315909239521346 40.0 38.0 41.0 34.0 41.0 22-23 38.148303145229846 40.0 37.0 41.0 33.5 41.0 24-25 38.158863532335054 40.0 37.0 41.0 33.5 41.0 26-27 37.9644543255084 40.0 37.0 41.0 33.0 41.0 28-29 37.7622441639966 40.0 36.5 41.0 33.0 41.0 30-31 37.625554175112796 39.0 36.0 41.0 33.0 41.0 32-33 37.345609102203625 39.0 35.0 41.0 33.0 41.0 34-35 37.114137186948284 39.0 35.0 41.0 33.0 41.0 36-37 36.87408618322108 39.0 35.0 41.0 32.5 41.0 38-39 36.640005231151505 39.0 35.0 41.0 31.5 41.0 40-41 36.41125351467992 39.0 35.0 41.0 31.0 41.0 42-43 36.087497547897726 39.0 35.0 41.0 30.5 41.0 44-45 35.59658013470215 38.0 35.0 40.0 29.5 41.0 46-47 35.0082325246845 38.0 35.0 40.0 26.0 41.0 48-49 34.4978290721245 38.0 35.0 40.0 23.0 41.0 50-51 34.13078532662002 38.0 34.0 40.0 20.5 41.0 52-53 33.89395802000915 38.0 34.0 40.0 19.0 41.0 54-55 33.63684692342902 38.0 34.0 40.0 14.0 41.0 56-57 33.30464264696266 38.0 33.0 40.0 8.0 41.0 58-59 32.98507160138625 38.0 33.0 40.0 2.0 41.0 60-61 32.54458248872033 37.0 33.0 40.0 2.0 41.0 62-63 32.137062708428694 37.0 32.0 39.5 2.0 41.0 64-65 31.70621199241483 36.0 31.5 39.0 2.0 40.5 66-67 31.191636696527823 36.0 31.0 39.0 2.0 40.0 68-69 30.682030994572678 35.0 30.5 38.5 2.0 40.0 70-71 30.22820244556333 35.0 29.5 38.0 2.0 40.0 72-73 29.692859478192638 35.0 29.0 37.0 2.0 39.0 74-75 29.145661413718695 34.0 29.0 37.0 2.0 39.0 76-77 27.887752566533706 32.5 27.0 35.0 2.0 37.5 78-79 28.437749297064016 34.0 28.0 36.0 2.0 38.0 80-81 28.18013470215131 34.0 28.0 35.5 2.0 37.0 82-83 27.764271235205648 34.0 26.5 35.0 2.0 37.0 84-85 27.333270123585955 34.0 26.0 35.0 2.0 36.0 86-87 26.9202707120905 33.0 26.0 35.0 2.0 36.0 88-89 26.49563852743085 33.0 25.0 35.0 2.0 35.5 90-91 25.92988949192441 33.0 23.5 35.0 2.0 35.0 92-93 25.42949715556137 32.0 20.5 35.0 2.0 35.0 94-95 24.915170339370952 32.0 18.0 34.0 2.0 35.0 96-97 24.31668737330805 32.0 4.5 34.0 2.0 35.0 98-99 23.495985091218202 31.0 2.0 34.0 2.0 35.0 100-101 21.00353756620676 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5776990461264475 1101 2 0.40331323509180805 1101 3 0.033749808928817515 1101 4 0.2671574017480367 1101 5 0.27645703988955006 1101 6 0.43911739547451845 1101 7 0.4656265143801761 1101 8 0.5477982657594751 1101 9 0.6466456873406798 1101 10-11 0.7832616251389695 1101 12-13 0.6654227032063886 1101 14-15 0.886205884260086 1101 16-17 0.5981786897718848 1101 18-19 0.4143663435321301 1101 20-21 0.18353709446126487 1101 22-23 0.41243192786833305 1101 24-25 0.4995298014532068 1101 26-27 0.528128102442075 1101 28-29 0.6536141644613664 1101 30-31 0.20108026050304773 1101 32-33 0.7915598408317663 1101 34-35 0.7056155238261042 1101 36-37 0.42336926956863863 1101 38-39 0.06297630580525748 1101 40-41 0.22455094386533148 1101 42-43 -0.17432545242728992 1101 44-45 -0.035745105329588966 1101 46-47 0.13366896743270473 1101 48-49 -0.38787041436685854 1101 50-51 0.26980382073701037 1101 52-53 0.3295759536875309 1101 54-55 0.23047450992918783 1101 56-57 0.3234173269867213 1101 58-59 0.16328722866069256 1101 60-61 0.6578656967738539 1101 62-63 0.46944441599253395 1101 64-65 0.5721266594604977 1101 66-67 -0.10405772218452469 1101 68-69 0.2019753088014724 1101 70-71 0.08230987996974548 1101 72-73 0.44355429462663665 1101 74-75 -0.12607334888166477 1101 76-77 0.300998023184043 1101 78-79 0.28721876907312804 1101 80-81 0.8708638213742894 1101 82-83 0.5668543269338961 1101 84-85 0.4378213465342107 1101 86-87 0.41698248532097537 1101 88-89 0.4606790442762261 1101 90-91 0.7514941475071026 1101 92-93 0.3888567548867279 1101 94-95 0.43083556466473993 1101 96-97 1.1286998153268257 1101 98-99 0.8671125283093097 1101 100-101 -0.11933462811861162 1102 1 0.11028833184073505 1102 2 0.01536680652037603 1102 3 0.17883909464310221 1102 4 -0.030610455394821656 1102 5 0.23895703988954864 1102 6 -0.12516831881119828 1102 7 -0.49330205704839614 1102 8 -0.012469591383378997 1102 9 0.2703064016263923 1102 10-11 0.022547339424683344 1102 12-13 -0.1316755110793224 1102 14-15 0.03308088426008027 1102 16-17 -0.5799463102281166 1102 18-19 -0.2456782993250144 1102 20-21 -0.3619986198244547 1102 22-23 0.09435157072547184 1102 24-25 -0.4185505556896487 1102 26-27 -0.36763082612934994 1102 28-29 -0.7202697641100642 1102 30-31 -0.47057152521124124 1102 32-33 -0.0725026591682365 1102 34-35 0.30226730954039027 1102 36-37 0.3506014124257817 1102 38-39 -0.24639869419474536 1102 40-41 0.42365808672247596 1102 42-43 0.37522811900128517 1102 44-45 0.010237037527552673 1102 46-47 -0.1455721039958675 1102 48-49 0.45520994277599414 1102 50-51 0.6807413207370132 1102 52-53 0.5362723822589643 1102 54-55 -0.1224272757850926 1102 56-57 0.09372982698672416 1102 58-59 0.24230508580355092 1102 60-61 0.27148176820242753 1102 62-63 -0.40220736972175075 1102 64-65 0.17458201660335604 1102 66-67 0.3620137063869038 1102 68-69 -1.3299443340556678 1102 70-71 -2.0056365486016823 1102 72-73 -1.5809992768019328 1102 74-75 -1.593037634595948 1102 76-77 -1.7190912625302417 1102 78-79 -2.207424088069729 1102 80-81 -1.7373951071971412 1102 82-83 -1.8356010302089594 1102 84-85 -2.0572679391800754 1102 86-87 -2.4640443003933115 1102 88-89 -2.211865598580914 1102 90-91 -1.7536397810643258 1102 92-93 -1.3497593165418458 1102 94-95 -0.9658162210495469 1102 96-97 -1.232684113244602 1102 98-99 -2.058780328833546 1102 100-101 -1.7780399852614686 1103 1 0.19854846697587192 1103 2 0.3342012659798357 1103 3 0.36000463518364256 1103 4 0.35325778784842043 1103 5 0.35213271556522585 1103 6 0.38158843794555963 1103 7 0.12663037538403898 1103 8 0.29876351672472623 1103 9 0.14741788811287648 1103 10-11 0.07997977185711846 1103 12-13 0.13627212405580735 1103 14-15 0.14161128966548375 1103 16-17 0.525205716798915 1103 18-19 0.2904281195939049 1103 20-21 -0.2564242955001319 1103 22-23 0.2850188004551981 1103 24-25 0.44779234971575477 1103 26-27 0.14144856576253773 1103 28-29 0.6236913845385814 1103 30-31 0.12212273154551667 1103 32-33 0.7807490300209565 1103 34-35 0.7399784581890358 1103 36-37 0.656766952966322 1103 38-39 -0.28567234284339094 1103 40-41 -0.23934867003428195 1103 42-43 0.310616632514801 1103 44-45 -0.30099607058055966 1103 46-47 -0.0499217661580289 1103 48-49 0.2611643346678818 1103 50-51 0.25358760452080276 1103 52-53 0.012201436313020508 1103 54-55 0.4069223863770688 1103 56-57 0.1382821918515873 1103 58-59 -0.06470504933157883 1103 60-61 0.6576726465808065 1103 62-63 0.21075715730528088 1103 64-65 0.9657560030898402 1103 66-67 0.9380272199004196 1103 68-69 0.8645235713497357 1103 70-71 0.9753600730199388 1103 72-73 0.5275311286034707 1103 74-75 -0.12935520216351648 1103 76-77 0.8125810347670566 1103 78-79 0.3044002362545939 1103 80-81 0.83418428469475 1103 82-83 1.2516033616829318 1103 84-85 1.8021070608199246 1103 86-87 1.2312681996066885 1103 88-89 1.2989029825001666 1103 90-91 1.209409205422162 1103 92-93 1.4904011564311297 1103 94-95 1.1775537113828882 1103 96-97 1.5420202786472892 1103 98-99 1.2275372387340227 1103 100-101 2.782981974197991 1104 1 0.3236873044434745 1104 2 0.545974683232707 1104 3 0.29891614943762335 1104 4 0.32919262679696004 1104 5 0.20385430016352046 1104 6 0.27551661269565386 1104 7 0.0161157511707799 1104 8 0.24544992916456465 1104 9 0.5376437303935191 1104 10-11 -0.023685537287640557 1104 12-13 0.6483972628932833 1104 14-15 0.4827812267258338 1104 16-17 0.6180417034705172 1104 18-19 0.4228790636886828 1104 20-21 0.3267856267508833 1104 22-23 0.5039192077117747 1104 24-25 0.5663595470500766 1104 26-27 0.513255304007636 1104 28-29 0.31408383178034427 1104 30-31 0.5229002213640968 1104 32-33 0.5230666901468339 1104 34-35 0.5194120013212142 1104 36-37 0.5066373713298944 1104 38-39 0.2944831551203251 1104 40-41 0.04480534699644778 1104 42-43 0.3861442148916936 1104 44-45 0.9509476539659119 1104 46-47 0.2047061494287945 1104 48-49 0.9103683331869519 1104 50-51 0.7335024508739991 1104 52-53 0.16362683431376013 1104 54-55 -0.7415411456481067 1104 56-57 -0.05192513876669835 1104 58-59 -0.40559731145672373 1104 60-61 -0.3987879235783893 1104 62-63 0.09468903438784082 1104 64-65 0.18817362619239475 1104 66-67 -0.0035684853939201844 1104 68-69 0.22966611114981106 1104 70-71 0.8366151637270853 1104 72-73 0.8139065451158771 1104 74-75 0.7498562010204886 1104 76-77 0.6180234634971562 1104 78-79 0.5417197475467077 1104 80-81 1.1374978722549152 1104 82-83 1.3973827026677519 1104 84-85 1.1959426772582837 1104 86-87 0.8374325831683329 1104 88-89 0.41987669593963517 1104 90-91 1.164703540853484 1104 92-93 0.9968802382526754 1104 94-95 1.0773130597723686 1104 96-97 0.9514982497690951 1104 98-99 1.608599808152757 1104 100-101 1.8029745695330526 1105 1 0.13484190326930445 1105 2 0.37474180652037603 1105 3 -0.0662501910711839 1105 4 0.3671574017480381 1105 5 0.2621713256038376 1105 6 -0.003739747382624614 1105 7 -0.034373485619823896 1105 8 0.34779826575947936 1105 9 0.43235997305496454 1105 10-11 0.4404044822818278 1105 12-13 0.41542270320638863 1105 14-15 0.2933487414029372 1105 16-17 0.5838929754861724 1105 18-19 0.3072234863892689 1105 20-21 0.04067995160411897 1105 22-23 0.12671764215404124 1105 24-25 0.41381551573892494 1105 26-27 0.806699531013507 1105 28-29 0.6893284501756511 1105 30-31 0.43679454621732816 1105 32-33 0.7772741265460468 1105 34-35 0.8984726666832472 1105 36-37 0.6019406981400692 1105 38-39 0.04869059151953792 1105 40-41 0.16026522957962186 1105 42-43 0.40424597614414637 1105 44-45 -0.035745105329588966 1105 46-47 0.05509753886127555 1105 48-49 0.6907010142045635 1105 50-51 -0.40876760783442023 1105 52-53 -0.3918526177410371 1105 54-55 -0.8338112043565218 1105 56-57 -0.583725530156137 1105 58-59 -0.5081413427678783 1105 60-61 -1.1064200175118586 1105 62-63 -0.8805555840074639 1105 64-65 -0.29930191196807243 1105 66-67 -0.5826291507559525 1105 68-69 -0.29802469119852404 1105 70-71 -0.8891186914588225 1105 72-73 -1.3778742768019328 1105 74-75 -1.0832162060245203 1105 76-77 -0.5275734053873862 1105 78-79 -0.11992408806973032 1105 80-81 -0.32199332148285364 1105 82-83 -0.3402885302089622 1105 84-85 -0.433607224894363 1105 86-87 -0.3544460861076004 1105 88-89 -0.3393209557237711 1105 90-91 -0.2199344239214689 1105 92-93 -0.3325718165418472 1105 94-95 -0.11202157819240455 1105 96-97 0.2501283867553994 1105 98-99 0.2385410997378834 1105 100-101 -0.5693346281186109 1106 1 -0.0070092033705329015 1106 2 0.40995307412600823 1106 3 -0.011521820849857534 1106 4 -0.07308404694411763 1106 5 -0.2601626784203077 1106 6 0.2515922445690819 1106 7 -0.031154169724452174 1106 8 -0.020612197017179312 1106 9 0.4637483030348406 1106 10-11 0.12642057886130686 1106 12-13 0.23705248187842187 1106 14-15 0.3559241941192326 1106 16-17 0.13550263343385183 1106 18-19 -0.17426544721234194 1106 20-21 -0.2955373522188225 1106 22-23 0.07289470452828795 1106 24-25 -0.2991623514642967 1106 26-27 -0.5567813542983657 1106 28-29 -0.3594643063635843 1106 30-31 -0.22618332098588922 1106 32-33 -0.4506936802950037 1106 34-35 -1.6868392045441212 1106 36-37 -1.489407390391122 1106 38-39 -1.4116715815186893 1106 40-41 -0.7220285329958287 1106 42-43 -0.4986715288860424 1106 44-45 -0.023873877965407075 1106 46-47 -0.8912807307564279 1106 48-49 -1.2293191065197817 1106 50-51 -1.2520271651784753 1106 52-53 -1.5911483923889236 1106 54-55 -1.8327045645174849 1106 56-57 -1.8005263349851077 1106 58-59 -1.961260055041521 1106 60-61 -1.4742268585581364 1106 62-63 -1.6613402922569591 1106 64-65 -1.636927666495236 1106 66-67 -1.656472209106056 1106 68-69 -1.966938172083836 1106 70-71 -1.769500985221402 1106 72-73 -1.9060432908864406 1106 74-75 -2.05122425431426 1106 76-77 -1.6466880935161576 1106 78-79 -2.1396423979288812 1106 80-81 -1.9767216917041814 1106 82-83 -1.495922333025863 1106 84-85 -0.9760619532645833 1106 86-87 -1.8919712370130313 1106 88-89 -3.081473873228802 1106 90-91 -1.9848237599375658 1106 92-93 -1.0741211123164938 1106 94-95 -2.300653368936871 1106 96-97 -1.1597307681741782 1106 98-99 -0.9280584978476298 1106 100-101 -0.2750690345572444 1107 1 -0.03658666815926637 1107 2 -0.13954390776533998 1107 3 -0.03767876249975188 1107 4 -0.4185568839662537 1107 5 0.34788561131811946 1107 6 0.3534031097602295 1107 7 -0.020087771334111437 1107 8 -0.15220173424052064 1107 9 -0.23906859837360628 1107 10-11 0.04040448228182214 1107 12-13 -0.006005868222175081 1107 14-15 -0.1352226871684863 1107 16-17 0.11960726120045706 1107 18-19 0.2572234863892717 1107 20-21 0.540679951604119 1107 22-23 0.5481462135826192 1107 24-25 0.6638155157389178 1107 26-27 0.642413816727796 1107 28-29 0.3893284501756469 1107 30-31 0.6296516890744783 1107 32-33 0.384416983688908 1107 34-35 0.37704409525467497 1107 36-37 -0.3837735875742183 1107 38-39 0.5558334486623977 1107 40-41 0.31026522957962044 1107 42-43 -0.8457540238558536 1107 44-45 -0.04288796247244875 1107 46-47 -0.5591881754244383 1107 48-49 0.21212958563313578 1107 50-51 0.612660963594152 1107 52-53 0.9367188108303921 1107 54-55 1.0519030813577643 1107 56-57 0.5091316127010117 1107 58-59 0.4632872286606897 1107 60-61 0.8864371253452887 1107 62-63 0.5123015588496784 1107 64-65 1.0006980880319283 1107 66-67 0.9602279921011956 1107 68-69 1.0305467373729016 1107 70-71 0.9394527371126031 1107 72-73 1.7864114374837818 1107 74-75 1.073926651118338 1107 76-77 0.5224265946126181 1107 78-79 0.8300759119302725 1107 80-81 0.6065781070885734 1107 82-83 0.8954257555053253 1107 84-85 0.6592499179627787 1107 86-87 0.6598396281781191 1107 88-89 -0.11789238429519955 1107 90-91 -0.15564870963575572 1107 92-93 -0.23971467368470556 1107 94-95 -0.5620215781924038 1107 96-97 0.47155695818396737 1107 98-99 -0.1757446145478312 1107 100-101 -0.08362034240432692 1108 1 0.38341333184073534 1108 2 0.16807513985371259 1108 3 0.43279742797643905 1108 4 0.35001454460518033 1108 5 0.0564570398895512 1108 6 0.2848316811887983 1108 7 0.3808646096182713 1108 8 0.2706554086166193 1108 9 0.2609314016263937 1108 10-11 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92-93 1.0880164187522716 2316 94-95 1.730415396597511 2316 96-97 1.3265989749906915 2316 98-99 1.7923226123429252 2316 100-101 2.499993102973825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 5.0 6 4.0 7 32.0 8 53.0 9 79.0 10 136.0 11 214.0 12 305.0 13 435.0 14 579.0 15 879.0 16 1183.0 17 1579.0 18 1342.0 19 804.0 20 754.0 21 755.0 22 822.0 23 813.0 24 860.0 25 913.0 26 981.0 27 1159.0 28 1340.0 29 1584.0 30 1907.0 31 2381.0 32 2949.0 33 3703.0 34 5080.0 35 7407.0 36 9858.0 37 14256.0 38 10584.0 39 729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.110659114988685 24.461419732900815 17.126002275967615 18.30191887614289 2 14.617145099064931 25.359314719152554 39.08977963774276 20.933760544039757 3 17.84607336689989 29.45530634931014 33.88086052442294 18.817759759367032 4 10.875563983521873 22.054534754462825 40.14516445432551 26.924736807689793 5 9.432802239489313 41.781126546843524 36.20464118462706 12.581430029040105 6 24.259465114758385 42.66004054142418 17.44458248872033 15.635911855097104 7 21.86621330020271 38.892303668344994 23.22369711632773 16.017785915124566 8 23.419865297848688 35.4515137644674 21.439874452363828 19.68874648532008 9 24.06591250899104 19.14078336493821 23.919440266788726 32.873863859282025 10-11 21.969528542470414 29.696593212580918 28.19132936637677 20.142548878571894 12-13 24.498443671366168 29.14323454787999 28.599173445633124 17.759148335120713 14-15 21.649120512652846 30.892565225920354 28.94461518341725 18.513699078009545 16-17 16.3323088994965 33.54802850977571 30.963839665206304 19.15582292552148 18-19 15.805924279081932 31.271169816255806 35.72353364284313 17.199372261819132 20-21 17.474007715948474 29.756751454914017 35.554175112796706 17.21506571634081 22-23 17.40742436032407 29.419534555251133 35.80877400623819 17.36426707818661 24-25 18.17367423003989 30.25044137840842 35.20761132544301 16.368273066108678 26-27 16.86654024717191 29.684823121689664 37.192833322435106 16.255803308703328 28-29 16.486735674724873 30.418691026554807 36.91647758109973 16.178095717620593 30-31 17.000588504544563 29.686784803504874 36.30157588439155 17.011050807559013 32-33 16.32315438435886 30.991303210619236 36.5984437324266 16.087098672595303 34-35 17.36480742823514 30.122278166481397 36.047865036291114 16.46504936899235 36-37 17.29876414045642 29.822794742692736 35.37762374942785 17.500817367423004 38-39 16.541554959785522 30.767671483685344 35.96547440005231 16.72529915647682 40-41 16.08131878191841 30.846340458644207 36.64249422934826 16.429846530089126 42-43 16.360830270609625 30.792471585335544 34.86861896229253 17.978079181762297 44-45 16.861309095664684 30.045118681749823 35.02648270450533 18.067089518080166 46-47 18.071012881710587 30.49565160530962 34.136533054338585 17.29680245864121 48-49 17.598247564245078 30.25436474203884 34.24442555417511 17.902962139540968 50-51 16.869809716863926 30.983456483358403 34.221539266331 17.925194533446675 52-53 17.222258549663245 31.906754724383706 33.35382201006997 17.517164715883084 54-55 17.95265807885961 30.498921075001633 32.779703132151965 18.768717713986792 56-57 18.26652716929314 31.25155299810371 31.922448178905384 18.55947165369777 58-59 18.29464460864448 30.09219904531485 33.136075328581704 18.477081017458968 60-61 17.783299548813183 31.876021709278756 32.76008631399987 17.580592427908194 62-63 17.47139214019486 31.50853331589616 33.29627934349049 17.723795200418493 64-65 17.49820179166939 31.328712482835286 33.13084417707448 18.042241548420847 66-67 17.364153534296737 31.326750801020076 31.829595239652132 19.47950042503106 68-69 17.221604655724843 30.455764075067027 32.93990714706075 19.382724122147387 70-71 18.46531092656771 30.43222389328451 32.6031517687831 18.499313411364675 72-73 17.87288301837442 29.356568364611256 33.49440920682665 19.276139410187668 74-75 17.26083829202903 31.139083240698355 31.939449421303866 19.660629045968744 76-77 17.22945138298568 29.710324985287386 33.43948211600078 19.620741515726152 78-79 17.052900019616818 29.471653697770222 33.55783691885176 19.917609363761198 80-81 18.241679199633822 30.28052049957497 31.855097103249854 19.62270319754136 82-83 17.710063427712026 29.584123455175572 32.769894723075915 19.935918394036488 84-85 16.88354148957039 29.416072713005953 32.81762898057935 20.88275681684431 86-87 17.402733276662524 30.228862878441117 31.984568103053686 20.383835741842674 88-89 17.89119204864971 29.178055319427187 31.889753481985224 21.040999149937882 90-91 17.5 27.985221030604237 32.87078210829192 21.643996861103844 92-93 18.536585365853657 29.234944092068265 32.20100699666514 20.027463545412935 94-95 17.7872229124436 28.140325639181324 32.996795919701825 21.07565552867325 96-97 17.589093049107436 28.562087229451382 32.30955339044007 21.539266331001112 98-99 17.74910741142775 29.21020624354263 31.524397420974847 21.51628892405477 100-101 17.481150151387336 27.904315356495925 32.67154506634231 21.942989425774428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 601.0 1 393.5 2 198.5 3 217.0 4 176.5 5 154.5 6 241.0 7 431.0 8 581.5 9 607.0 10 502.5 11 366.0 12 305.5 13 263.0 14 262.5 15 291.5 16 300.0 17 271.5 18 248.0 19 268.5 20 279.5 21 312.0 22 357.0 23 360.0 24 405.0 25 503.5 26 718.0 27 954.5 28 983.0 29 1052.5 30 1344.5 31 1626.0 32 2138.0 33 2834.5 34 3248.5 35 3595.5 36 4206.5 37 4779.5 38 4547.5 39 3759.0 40 3252.0 41 2825.0 42 2232.5 43 2148.5 44 2278.0 45 2129.0 46 1834.0 47 2119.0 48 3361.0 49 3156.0 50 1773.0 51 997.0 52 586.0 53 447.5 54 387.0 55 348.5 56 295.0 57 252.5 58 237.0 59 213.0 60 200.5 61 186.0 62 152.5 63 114.0 64 85.0 65 63.0 66 48.0 67 49.5 68 46.0 69 43.0 70 35.5 71 30.0 72 26.0 73 28.5 74 27.5 75 19.0 76 16.5 77 11.0 78 7.5 79 5.5 80 3.0 81 3.0 82 2.5 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018309030275289347 2 0.0 3 0.0 4 0.0 5 0.024847969659321262 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003923363630419146 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.53893938403191E-4 24-25 0.0 26-27 0.0 28-29 6.53893938403191E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.53893938403191E-4 42-43 0.010462303014451056 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00653893938403191 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.002615575753612764 100-101 0.0071928333224351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 76465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.64545870659779 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76013526645158 24.480481265938664 2 10.199401407082053 6.863270777479894 3 4.201811326621837 4.241156084483097 4 2.46822404477786 3.3217812070882107 5 1.7530221168422282 2.949061662198391 6 1.302133944882808 2.6286536323808276 7 0.9250981459167411 2.1787746027594324 8 0.7968282349282855 2.1447721179624666 9 0.6063668519454269 1.8361341790361603 >10 4.539977455591402 29.42915059177401 >50 0.31873129397131417 6.923429019812986 >100 0.10883507599020485 6.728568626168835 >500 0.01166090099895052 3.196233570914798 >1k 0.007773933999300346 3.0785326620022233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 1.56149872490682 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 1.5170339370954031 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 975 1.2750931798862224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 1.0619237559667822 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 657 0.859216635061793 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 483 0.6316615444974826 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 387 0.5061139083240698 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.37402733276662525 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.36618060550578696 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 258 0.33740927221604655 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 214 0.27986660563656574 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 191 0.24978748447001897 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 186 0.24324854508598706 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 185 0.24194075720918068 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 184 0.2406329693323743 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.23540181782514877 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176 0.2301706663179232 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 176 0.2301706663179232 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 167 0.2184005754266658 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 162 0.2118616360426339 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 155 0.2027071209049892 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 140 0.18309030275289348 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 137 0.17916693912247433 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 134 0.1752435754920552 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 133 0.1739357876152488 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.17132021186163604 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 126 0.16478127247760413 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 120 0.15693454521676584 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 119 0.15562675733995945 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 115 0.15039560583273393 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 111 0.14516445432550842 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 108 0.14124109069508928 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 101 0.13208657555744457 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 99 0.1294709998038318 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 97 0.12685542405021905 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 96 0.12554763617341266 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 94 0.1229320604197999 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 93 0.12162427254299353 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 93 0.12162427254299353 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 88 0.1150853331589616 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 87 0.11377754528215524 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 82 0.10723860589812334 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 82 0.10723860589812334 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 81 0.10593081802131694 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 81 0.10593081802131694 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.10462303014451056 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 80 0.10462303014451056 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 77 0.10069966651409142 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 77 0.10069966651409142 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.53893938403191E-4 0.0 18-19 0.0 0.0 0.0 0.001307787876806382 0.0 20-21 0.0 0.0 0.0 0.003269469692015955 0.0 22-23 0.0 0.0 0.0 0.00653893938403191 0.0 24-25 0.0 0.0 0.0 0.011770090891257438 0.0 26-27 0.0 0.0 0.0 0.017655136336886156 0.0 28-29 0.0 0.0 0.0 0.03792584842738508 0.0 30-31 0.0 0.0 0.0 0.07192833322435101 0.0 32-33 0.0 0.0 0.0 0.1092002877133329 0.0 34-35 0.0 0.0 0.0 0.157588439155169 0.0 36-37 0.0 0.0 0.0 0.2118616360426339 0.0 38-39 0.0 0.0 0.0 0.30340678741908067 0.0 40-41 0.0 0.0 0.0 0.37206565095141564 0.0 42-43 0.0 0.0 0.0 0.4341855750997188 0.0 44-45 0.0 0.0 0.0 0.47080363565029754 0.0 46-47 0.0 0.0 0.0 0.5303079840449879 0.0 48-49 0.0 0.0 0.0 0.5871967566860655 0.0 50-51 0.0 0.0 0.0 0.6395082717583208 0.0 52-53 0.0 0.0 0.0 0.6944353625841888 0.0 54-55 0.0 0.0 0.0 0.818021316942392 0.0 56-57 0.0 0.0 0.0 0.9703786045903355 0.0 58-59 0.0 0.0 0.0 1.183548028509776 0.0 60-61 0.0 0.0 0.0 1.3600993918786373 0.0 62-63 0.0 0.0 0.0 1.5320734976786765 0.0 64-65 0.0 0.0 0.0 1.73151114889165 0.0 66-67 0.0 0.0 0.0 2.0087621787746026 0.0 68-69 0.0 0.0 0.0 2.334401360099392 0.0 70-71 0.0 0.0 0.0 2.69665860197476 0.0 72-73 0.0 0.0 0.0 3.040606813574838 0.0 74-75 0.0 0.0 0.0 3.450598312953639 0.0 76-77 0.0 0.0 0.0 3.8200483881514415 0.0 78-79 0.0 0.0 0.0 4.201922448178905 0.0 80-81 0.0 0.0 0.0 4.587065977898385 0.0 82-83 0.0 0.0 0.0 4.982017916693913 0.0 84-85 0.0 0.0 0.0 5.531288824952592 0.0 86-87 0.0 0.0 0.0 6.1557575361276395 0.0 88-89 6.53893938403191E-4 0.0 0.0 6.825344929052507 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTGCA 15 6.5941137E-4 95.0 7 ATGGGGC 20 0.002066656 71.25 5 ATAGAAA 25 0.0050034127 57.0 6 GAAACCT 25 0.0050034127 57.0 9 AGATATA 35 2.771472E-4 54.285717 8 GTAGATA 35 2.771472E-4 54.285717 6 TAGATAT 40 5.356488E-4 47.499996 7 TGTAGAT 40 5.356488E-4 47.499996 5 CATGTAG 45 9.568713E-4 42.22222 3 AGGGGAA 30 0.0039403397 31.666668 94-95 AGTAGTG 40 4.4756415E-4 29.687498 92-93 CATGGGC 65 0.0058104564 29.23077 4 CATGGGG 115 3.8028847E-6 28.913044 4 GGGCTTC 35 0.008369233 27.142859 24-25 GGCAAGT 90 8.871065E-4 26.388887 7 TATACTA 90 8.871065E-4 26.388887 5 GTACATG 435 0.0 24.022987 1 GCAAGTG 100 0.0016396152 23.75 8 CGAACAT 50 0.0016471199 23.75 84-85 AATTTGA 50 0.0016471199 23.75 14-15 >>END_MODULE