##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937665_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74378 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.660813681464948 33.0 31.0 34.0 30.0 34.0 2 31.794052004625023 33.0 31.0 34.0 30.0 34.0 3 31.75650057812794 33.0 31.0 34.0 30.0 34.0 4 35.36612976955551 37.0 35.0 37.0 33.0 37.0 5 35.35872166500847 37.0 35.0 37.0 33.0 37.0 6 35.469170991422196 37.0 35.0 37.0 33.0 37.0 7 35.44089650165372 37.0 35.0 37.0 33.0 37.0 8 35.44123262254968 37.0 35.0 37.0 33.0 37.0 9 37.03393476565651 39.0 37.0 39.0 34.0 39.0 10-11 36.93899405738256 39.0 37.0 39.0 33.0 39.0 12-13 36.87083546209901 39.0 37.0 39.0 33.0 39.0 14-15 37.921253596493585 40.0 38.0 41.0 33.0 41.0 16-17 37.87861329963161 40.0 38.0 41.0 33.0 41.0 18-19 37.90264594369303 40.0 38.0 41.0 33.0 41.0 20-21 37.87318830837076 40.0 38.0 41.0 33.0 41.0 22-23 37.703171636774314 40.0 37.5 41.0 33.0 41.0 24-25 37.520483207400034 40.0 37.0 41.0 33.0 41.0 26-27 37.406134878593136 40.0 37.0 41.0 32.5 41.0 28-29 37.38688187367232 40.0 37.0 41.0 33.0 41.0 30-31 37.16829573260911 39.0 36.0 41.0 33.0 41.0 32-33 36.79769555513727 39.0 35.0 41.0 32.0 41.0 34-35 36.68989486138375 39.0 35.0 41.0 31.5 41.0 36-37 36.5176799591277 39.0 35.0 41.0 31.0 41.0 38-39 36.31724434644653 39.0 35.0 41.0 31.0 41.0 40-41 36.08022533544865 39.0 35.0 41.0 30.5 41.0 42-43 35.718868482615825 39.0 35.0 41.0 30.0 41.0 44-45 35.30679098658206 38.5 35.0 40.0 27.5 41.0 46-47 34.798314017585845 38.0 35.0 40.0 24.5 41.0 48-49 34.31509317271236 38.0 35.0 40.0 21.5 41.0 50-51 33.294946086208284 37.5 33.5 39.5 16.5 40.5 52-53 33.384690365430636 38.0 33.5 39.5 14.0 40.5 54-55 33.69614671004867 38.0 34.0 40.0 8.5 41.0 56-57 33.425078652289656 38.0 34.0 40.0 2.0 41.0 58-59 33.17048051843287 38.0 33.5 40.0 2.0 41.0 60-61 32.83261851622792 38.0 33.0 40.0 2.0 41.0 62-63 32.407593643281615 37.0 33.0 40.0 2.0 41.0 64-65 32.050781144962215 37.0 32.5 39.5 2.0 41.0 66-67 31.55980262980989 36.0 31.5 39.0 2.0 41.0 68-69 31.023851138777594 36.0 31.0 39.0 2.0 40.0 70-71 30.551829842157627 35.0 30.5 38.5 2.0 40.0 72-73 30.04698297883783 35.0 30.0 37.5 2.0 40.0 74-75 29.563486514829656 35.0 29.0 37.0 2.0 39.0 76-77 29.056374196671058 35.0 29.0 36.5 2.0 39.0 78-79 28.508403022399097 34.0 28.5 36.0 2.0 38.5 80-81 27.94857350291753 34.0 26.5 36.0 2.0 37.5 82-83 27.412803517169053 34.0 26.0 35.0 2.0 37.0 84-85 26.897093226491705 34.0 25.5 35.0 2.0 36.5 86-87 26.486729947027346 33.5 24.5 35.0 2.0 36.0 88-89 25.934866492780124 33.0 22.0 35.0 2.0 36.0 90-91 25.46680470031461 33.0 19.5 35.0 2.0 35.0 92-93 24.91831589986286 32.0 15.0 35.0 2.0 35.0 94-95 24.313735244292666 32.0 4.5 34.5 2.0 35.0 96-97 23.614697894538708 31.5 2.0 34.0 2.0 35.0 98-99 22.79779639140606 31.0 2.0 34.0 2.0 35.0 100-101 20.633419828443895 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1227660513473232 1101 2 1.0404073215735998 1101 3 1.0608462358295156 1101 4 1.0943885582234643 1101 5 0.9911285931741176 1101 6 1.0271927326952977 1101 7 1.2609084480946393 1101 8 0.9710386297981373 1101 9 1.2439141359194252 1101 10-11 1.3459040239177114 1101 12-13 0.8740250944913868 1101 14-15 0.5501445818631723 1101 16-17 1.5191969551922213 1101 18-19 1.7622855914042574 1101 20-21 1.3992493457367772 1101 22-23 1.519465731204889 1101 24-25 1.5604525568003709 1101 26-27 1.9695553953678484 1101 28-29 1.729864306669569 1101 30-31 1.7701632457423955 1101 32-33 1.5872242609832128 1101 34-35 1.6022061154735 1101 36-37 2.0921394087633303 1101 38-39 2.0133912301726227 1101 40-41 2.204109395195559 1101 42-43 2.2681319112368783 1101 44-45 2.2743394285307375 1101 46-47 1.430046207103608 1101 48-49 0.9242780319085497 1101 50-51 1.609189872376092 1101 52-53 1.548017119088243 1101 54-55 1.774223094796774 1101 56-57 1.7431727837759965 1101 58-59 1.8105009279897857 1101 60-61 1.8470151037439493 1101 62-63 1.6842767925523034 1101 64-65 1.8438623623893378 1101 66-67 1.7438451809543452 1101 68-69 1.6135808594002263 1101 70-71 2.249820561303622 1101 72-73 1.7829979014019237 1101 74-75 2.5006985587229202 1101 76-77 2.1695792405721512 1101 78-79 2.4893774531144324 1101 80-81 2.3427578109580374 1101 82-83 2.457412639888055 1101 84-85 1.1866640557065153 1101 86-87 1.4892516895047905 1101 88-89 1.4249513819857604 1101 90-91 1.025992146164679 1101 92-93 2.0243193052908772 1101 94-95 2.285547547819476 1101 96-97 1.570950234810475 1101 98-99 2.244884613606825 1101 100-101 3.0529216217937787 1102 1 0.36443271801399035 1102 2 0.013697919864195995 1102 3 0.3997351247184042 1102 4 0.34118343001834006 1102 5 0.02467560172112826 1102 6 0.2600987156012806 1102 7 -0.19678385959766587 1102 8 0.3503121340716362 1102 9 0.042418409423696346 1102 10-11 0.5766732546869378 1102 12-13 -0.11219285422655645 1102 14-15 0.5206574023759885 1102 16-17 0.8945174680127366 1102 18-19 0.8321573862760516 1102 20-21 0.708651055138489 1102 22-23 0.9968161585553119 1102 24-25 0.8515850354328478 1102 26-27 0.23301693382938993 1102 28-29 -0.1970587702535056 1102 30-31 0.6952700833492358 1102 32-33 1.2420319532909048 1102 34-35 1.4024197906871692 1102 36-37 1.324724878848805 1102 38-39 0.854737384018776 1102 40-41 0.29972905331521105 1102 42-43 0.34377293687789745 1102 44-45 1.2130146422059482 1102 46-47 1.411563301120701 1102 48-49 1.6051541002846292 1102 50-51 1.342095855282075 1102 52-53 0.9817778028489315 1102 54-55 0.5338384794121609 1102 56-57 0.5724462880494983 1102 58-59 1.0619966544855188 1102 60-61 0.424792881521725 1102 62-63 0.9609861942617073 1102 64-65 1.2193965504235322 1102 66-67 1.2657468903560556 1102 68-69 1.3778970987164634 1102 70-71 1.246401757884815 1102 72-73 1.7274423458463666 1102 74-75 1.6270874476118067 1102 76-77 0.15675872775164024 1102 78-79 0.11256121379819106 1102 80-81 0.9343176400178628 1102 82-83 1.5154254604008734 1102 84-85 1.948202517244976 1102 86-87 1.522691860444965 1102 88-89 2.033071040105419 1102 90-91 2.275671633344171 1102 92-93 2.435750929222504 1102 94-95 2.4124706247425536 1102 96-97 3.050010063870303 1102 98-99 2.917961536683748 1102 100-101 4.085079741451899 1103 1 0.541516051347326 1103 2 0.8661284754197531 1103 3 0.7608462358295185 1103 4 0.8359750966850044 1103 5 0.3093978239433497 1103 6 0.37815427115683775 1103 7 -0.13428385959766587 1103 8 -0.25732675481724954 1103 9 -0.12772047946518938 1103 10-11 0.16261075468693775 1103 12-13 0.6586404791067721 1103 14-15 0.5414907357093242 1103 16-17 0.8450383013460652 1103 18-19 0.09257405294271592 1103 20-21 0.0749704995829319 1103 22-23 0.7798022696664262 1103 24-25 -0.01647052012270933 1103 26-27 0.19395443382938993 1103 28-29 -0.5720587702535056 1103 30-31 0.004297861127014357 1103 32-33 0.04671945329090477 1103 34-35 -0.8623371537572737 1103 36-37 -0.5624278989289735 1103 38-39 -0.49682511598122403 1103 40-41 0.23896516442632532 1103 42-43 0.6198146035445689 1103 44-45 -0.07517980223849463 1103 46-47 -0.5033672544348562 1103 48-49 -0.4842556219375993 1103 50-51 -0.5945360891623679 1103 52-53 -1.10763191937329 1103 54-55 -1.114599020587839 1103 56-57 -1.8008176008393875 1103 58-59 -2.3772394566255954 1103 60-61 -1.9623598962560536 1103 62-63 -1.8836318612938499 1103 64-65 -1.290152060687582 1103 66-67 -1.0007461651995015 1103 68-69 -0.8061306790613152 1103 70-71 -1.3924871310040743 1103 72-73 -1.6388770985980763 1103 74-75 -1.2696139412770826 1103 76-77 -1.1218871055816955 1103 78-79 -1.3127860084240304 1103 80-81 -1.43200180442658 1103 82-83 -1.9715537062657909 1103 84-85 -1.161172482755024 1103 86-87 -1.571926195110592 1103 88-89 -1.7698803487834702 1103 90-91 -1.5810991999891648 1103 92-93 -1.5217143485552747 1103 94-95 -1.2594043752574464 1103 96-97 -0.6444343805741397 1103 98-99 -0.5820384633162519 1103 100-101 -0.28037164743698995 1104 1 -0.4018716298120957 1104 2 -0.41444217675416084 1104 3 0.3478027575686511 1104 4 -0.4830919323005034 1104 5 -0.6824500021436037 1104 6 -0.5068094969591073 1104 7 -0.693160671191869 1104 8 -0.2976347258317418 1104 9 -0.08741250845069715 1104 10-11 -0.3726021076319057 1104 12-13 -0.515272564371493 1104 14-15 0.08949798208613657 1104 16-17 -0.5737796334365441 1104 18-19 -0.8037121789413462 1104 20-21 0.1530954995829319 1104 22-23 -0.5841017158408235 1104 24-25 -0.16864443316618605 1104 26-27 -0.6311135009532194 1104 28-29 -0.9905370311230683 1104 30-31 -1.170973878003423 1104 32-33 -0.8059752930859077 1104 34-35 -1.3771695812935079 1104 36-37 -1.8054079713927393 1104 38-39 -1.3472553696044116 1104 40-41 -1.1281091109359949 1104 42-43 -1.1960821355858684 1104 44-45 -1.3274442949921195 1104 46-47 -0.6648255877681848 1104 48-49 -0.3426116001984667 1104 50-51 -0.3378558355391803 1104 52-53 -0.8020386222718372 1104 54-55 -0.051306448124073256 1104 56-57 0.05131102234901874 1104 58-59 0.3766101810555611 1104 60-61 0.9296237993961185 1104 62-63 0.7333291894307905 1104 64-65 0.24687239583415277 1104 66-67 0.7527637985686155 1104 68-69 1.1707714948517314 1104 70-71 0.5893969269669412 1104 72-73 0.4786500753149667 1104 74-75 0.2862058050997298 1104 76-77 0.5118311915197538 1104 78-79 0.8278389915759696 1104 80-81 -0.19581521022368165 1104 82-83 -0.09598758307738464 1104 84-85 0.2670430969551205 1104 86-87 0.35391166720824785 1104 88-89 0.6767908468687018 1104 90-91 -0.30132112027902025 1104 92-93 0.03569054274907302 1104 94-95 -0.1056453172864309 1104 96-97 1.761362720875134 1104 98-99 1.921584725089545 1104 100-101 2.83296621488185 1105 1 0.9551189925237971 1105 2 0.8266064165962241 1105 3 0.9373168240648155 1105 4 0.4416736260967724 1105 5 0.7689566474727627 1105 6 0.7421248593921348 1105 7 0.7443926109905661 1105 8 0.8511291275356925 1105 9 0.9402942264171656 1105 10-11 1.0987320782163437 1105 12-13 0.9086404791067721 1105 14-15 1.3392848533563821 1105 16-17 0.7388802131107681 1105 18-19 1.3002946411780059 1105 20-21 0.6604484407594029 1105 22-23 1.0638096226076001 1105 24-25 0.6673530092890587 1105 26-27 1.291840463241158 1105 28-29 0.8911765238641465 1105 30-31 0.41790080230348536 1105 32-33 0.1060025415261947 1105 34-35 1.1785635815368423 1105 36-37 1.1241529834239685 1105 38-39 0.7554726781364209 1105 40-41 0.9586342820733833 1105 42-43 0.36338078001515584 1105 44-45 0.15092313893796927 1105 46-47 0.33762539262396984 1105 48-49 0.3277848192388717 1105 50-51 0.15500435201410312 1105 52-53 0.28896734533259405 1105 54-55 0.5485443617651029 1105 56-57 0.7938515168076705 1105 58-59 -0.3579379860373635 1105 60-61 -0.31622019037369853 1105 62-63 -0.1460399495291398 1105 64-65 -0.152743972452285 1105 66-67 0.03004427597696946 1105 68-69 0.7618840268210398 1105 70-71 0.4678069866429837 1105 72-73 0.4815273131666302 1105 74-75 0.13893385284056237 1105 76-77 0.12489598265360158 1105 78-79 0.1807801680465566 1105 80-81 0.3906084896910649 1105 82-83 0.22743526432244465 1105 84-85 0.05114369371556293 1105 86-87 0.809048069595292 1105 88-89 0.5853586218047653 1105 90-91 1.0930735941284802 1105 92-93 1.1101790337976674 1105 94-95 1.0080588600366696 1105 96-97 1.5099773841317443 1105 98-99 2.256196830801393 1105 100-101 2.016043793739481 1106 1 0.10609938468066105 1106 2 -0.1224131912469133 1106 3 0.21084623582951778 1106 4 0.14951676335167008 1106 5 0.32189782394335253 1106 6 -0.20101239550982797 1106 7 0.2865494737356684 1106 8 0.40308991184942045 1106 9 0.14519618720147776 1106 10-11 -0.3733267453130651 1106 12-13 -0.4330261875598964 1106 14-15 0.1373240690426556 1106 16-17 0.09868413467940229 1106 18-19 -0.15950928039061552 1106 20-21 -0.0969045004170681 1106 22-23 -0.7254060636669095 1106 24-25 0.008529479877296353 1106 26-27 -0.1336497328372772 1106 28-29 0.16127456307982868 1106 30-31 -0.03111880553964852 1106 32-33 0.3211986199575705 1106 34-35 0.5163086795760634 1106 36-37 0.642780434404358 1106 38-39 0.5839040506854474 1106 40-41 0.6316734977596568 1106 42-43 0.15210627021123457 1106 44-45 0.09357019776150821 1106 46-47 1.0601744122318095 1106 48-49 1.5037652113957378 1106 50-51 1.597651410837635 1106 52-53 1.4262222472933814 1106 54-55 1.5171718127454952 1106 56-57 1.6918907324939454 1106 58-59 1.7175522100410703 1106 60-61 2.430348437077278 1106 62-63 2.4304306387061487 1106 64-65 2.362452105979081 1106 66-67 1.7143580014671613 1106 68-69 1.5667859876053534 1106 70-71 2.0658462023292543 1106 72-73 2.7246645680685866 1106 74-75 2.6673652253895845 1106 76-77 2.723425394418303 1106 78-79 2.8361723249093025 1106 80-81 2.8523731955734206 1106 82-83 2.294592127067542 1106 84-85 2.3648691839116402 1106 86-87 3.037969638222741 1106 88-89 2.8525154845498655 1106 90-91 1.9798383000108366 1106 92-93 2.5704731514447268 1106 94-95 3.4041372914092207 1106 96-97 3.822232286092529 1106 98-99 4.334628203350412 1106 100-101 4.533690852563009 1107 1 0.24381868292627473 1107 2 -0.09522020879077431 1107 3 -0.37073271153890275 1107 4 -0.41978148226236556 1107 5 0.20084519236440457 1107 6 -0.05276678147474456 1107 7 -0.15731017538713843 1107 8 -0.16357675481724954 1107 9 0.1601084679032283 1107 10-11 -0.6009583242604322 1107 12-13 7.457422646623968E-4 1107 14-15 -0.5604829485011962 1107 16-17 -0.14385972496972244 1107 18-19 0.02513984241640088 1107 20-21 0.04783234168819206 1107 22-23 -0.07628325664936852 1107 24-25 -0.49673367801744917 1107 26-27 0.41064851277675984 1107 28-29 0.39504649290438465 1107 30-31 0.27896891375858957 1107 32-33 1.0676898480277472 1107 34-35 0.6882385041374661 1107 36-37 0.017341837913129154 1107 38-39 0.4632900155977211 1107 40-41 1.1246559539000103 1107 42-43 1.0959659193340414 1107 44-45 1.755412303024663 1107 46-47 1.8329814297756641 1107 48-49 1.563852930693976 1107 50-51 1.5292303582060498 1107 52-53 1.3253450543109224 1107 54-55 1.4483121636226812 1107 56-57 1.4730310833711329 1107 58-59 1.5232539644270346 1107 60-61 1.7391203669018367 1107 62-63 2.0435885334429855 1107 64-65 2.3023643866808428 1107 66-67 2.2266387032215533 1107 68-69 2.5803824788334246 1107 70-71 2.0351444479432956 1107 72-73 2.829050532980869 1107 74-75 2.8270143481966024 1107 76-77 3.3234253944183045 1107 78-79 3.79494425473386 1107 80-81 3.37474161662605 1107 82-83 3.4178377411026304 1107 84-85 2.928465675139716 1107 86-87 3.128320515415723 1107 88-89 2.683655835427057 1107 90-91 3.1429961947476777 1107 92-93 2.812578414602619 1107 94-95 2.919049572110975 1107 96-97 3.0989866720574426 1107 98-99 3.194277326157433 1107 100-101 2.7981645367735375 1108 1 -0.4179746893934144 1108 2 -0.4971045492716044 1108 3 -0.4181661098494942 1108 4 -0.39184126133968533 1108 5 -0.5509416822294924 1108 6 -0.5701481979789662 1108 7 -0.3233270694742103 1108 8 -0.8209841622246543 1108 9 -0.39122356588494256 1108 10-11 -0.42332674531306225 1108 12-13 -0.3722237184240882 1108 14-15 -0.4145277828091949 1108 16-17 -0.20501956902430152 1108 18-19 -0.8638302680449428 1108 20-21 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-3.398096621999972 2316 76-77 -3.2970565332925403 2316 78-79 -3.2083055867372856 2316 80-81 -3.307566563462725 2316 82-83 -3.1128375918079563 2316 84-85 -2.816857723718879 2316 86-87 -3.14355646619493 2316 88-89 -2.9431672463738323 2316 90-91 -1.7285954349289234 2316 92-93 -1.7885629931335885 2316 94-95 -1.723071543932143 2316 96-97 -2.9034705251524535 2316 98-99 -2.241677017533121 2316 100-101 -2.3151043281598795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 597.0 3 95.0 4 75.0 5 57.0 6 83.0 7 76.0 8 94.0 9 108.0 10 142.0 11 224.0 12 300.0 13 417.0 14 499.0 15 796.0 16 1056.0 17 1360.0 18 1172.0 19 723.0 20 722.0 21 719.0 22 811.0 23 850.0 24 894.0 25 871.0 26 968.0 27 1129.0 28 1300.0 29 1503.0 30 1766.0 31 2147.0 32 2667.0 33 3489.0 34 4731.0 35 7033.0 36 9246.0 37 13403.0 38 11041.0 39 1214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.909090909090914 23.98937461233516 16.307542946522478 18.793991532051454 2 14.221964569795345 25.1867231105239 39.198371396985465 21.392940922695285 3 17.844577744827124 30.07211700478533 32.774819707488035 19.308485542899508 4 10.971829087478097 21.29667071033832 40.8451273756571 26.886372826526483 5 9.703765600150946 41.9081376856519 35.70311868244427 12.684978031752877 6 24.30582687773661 42.63523071741327 17.756820478275515 15.302121926574605 7 22.34360687537044 38.55945902257665 23.491298022522766 15.605636079530147 8 23.0276984426362 35.49738643099639 21.623915503583554 19.850999622783856 9 24.47423374873695 19.72381273155945 23.94476254631189 31.857190973391713 10-11 21.74721440023713 30.164643429757078 28.040581506581695 20.0475606634241 12-13 24.133517464380745 29.8339452322409 28.220553066792416 17.81198423658594 14-15 21.6984500144827 31.53927505675197 28.20825446437593 18.554020464389403 16-17 16.64477628025083 34.48038958152317 30.041692766742777 18.833141371483226 18-19 16.150151889696424 32.97050443544856 34.12836868445336 16.750974990401648 20-21 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32.58954429731805 18.230801352425342 46-47 17.9323118370096 32.3374305438626 32.39939383734635 17.330863781781446 48-49 17.285605545189387 32.16977764006116 32.49648036752373 18.048136447225723 50-51 16.53979331994988 32.53055065278021 32.83167836596112 18.097977661308796 52-53 16.827353882432206 33.30570995452249 31.97978777160182 17.88714839144349 54-55 17.719685845536233 31.965755546873947 31.514461613072704 18.800096994517116 56-57 18.19602033601562 32.64603885391064 30.45352008349887 18.704420726574863 58-59 18.029982624624544 31.291838961248875 31.742386487614993 18.93579192651159 60-61 17.892765980374328 32.31298702173371 32.00519938846571 17.789047609426248 62-63 17.20224423953822 32.56976203786649 32.39598838814837 17.832005334446922 64-65 17.346505848839325 32.36761823948926 32.08342593153794 18.202449980133476 66-67 17.498417316577093 32.021389798090006 30.94381810589836 19.536374779434546 68-69 17.022279695034886 31.378808696354966 32.3028637624936 19.296047846116544 70-71 18.412456812656163 30.978374337457314 32.16438466874549 18.44478418114102 72-73 17.612909308872457 30.304683395967185 32.915774302609066 19.166632992551285 74-75 17.033270474137932 32.211745689655174 31.590113146551722 19.16487068965517 76-77 17.128597503889647 30.802648328629832 32.46425227822268 19.604501889257836 78-79 17.012104352043327 30.72093978808964 32.63257869176002 19.63437716810702 80-81 18.162958771983 31.103208120323032 31.120720429455705 19.613112678238263 82-83 17.949011551544135 30.525039571616208 31.874179099451048 19.651769777388612 84-85 17.09815595576449 30.41325987688412 32.0215789544579 20.467005212893493 86-87 17.562670905343698 31.088944865767743 31.489014911701712 19.859369317186847 88-89 18.222778978845493 30.27364139036497 31.102041366119117 20.401538264670425 90-91 18.21402353194728 29.086268091784024 31.615245253537537 21.08446312273116 92-93 19.219915000235734 30.45039098544497 31.13604677007631 19.193647244242985 94-95 18.599168850062973 29.134027978904687 32.037664428264485 20.229138742767848 96-97 18.171410870253272 29.225506239183286 31.814110722775546 20.7889721677879 98-99 18.423764083155437 30.082226098199914 30.74016957028277 20.753840248361875 100-101 18.733038377879687 28.568927318397 31.966302349542413 20.731731954180894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 573.0 1 354.0 2 155.5 3 181.0 4 157.0 5 145.0 6 244.0 7 411.5 8 538.5 9 584.0 10 484.0 11 347.0 12 308.5 13 325.5 14 329.0 15 285.5 16 251.5 17 257.5 18 276.5 19 285.5 20 315.0 21 350.0 22 353.0 23 380.0 24 429.0 25 478.0 26 669.0 27 934.5 28 1003.5 29 1064.0 30 1343.5 31 1671.0 32 2213.0 33 2792.5 34 3179.5 35 3598.0 36 4191.0 37 4640.0 38 4323.5 39 3664.0 40 3137.5 41 2628.0 42 2081.0 43 2031.0 44 2151.5 45 2016.5 46 1790.0 47 2002.0 48 3136.0 49 2910.5 50 1554.0 51 886.5 52 548.5 53 432.5 54 394.5 55 353.5 56 306.5 57 258.0 58 265.0 59 259.0 60 227.0 61 202.5 62 174.5 63 139.5 64 98.0 65 88.5 66 73.5 67 68.5 68 64.5 69 50.0 70 38.0 71 31.0 72 29.0 73 29.5 74 38.0 75 34.5 76 18.0 77 11.0 78 8.0 79 4.5 80 2.5 81 1.0 82 0.5 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29040845411277527 2 0.27427465110651 3 0.2594853316841001 4 0.2527629137648229 5 0.2420070450939794 6 0.20570598832988252 7 0.19091666890747264 8 0.20167253757831616 9 0.20570598832988252 10-11 0.21108392266530424 12-13 0.2090671972895211 14-15 0.20368926295409934 16-17 0.19427787786711123 18-19 0.19696684503482215 20-21 0.18486649278012315 22-23 0.18016080023662912 24-25 0.18016080023662912 26-27 0.19091666890747264 28-29 0.2090671972895211 30-31 0.20570598832988252 32-33 0.21175616445723194 34-35 0.19898357041060527 36-37 0.1794885584447014 38-39 0.1929333942832558 40-41 0.18284976740434 42-43 0.19158891069940034 44-45 0.1895721853236172 46-47 0.18889994353168948 48-49 0.20368926295409934 50-51 0.21108392266530424 52-53 0.22251203312807552 54-55 0.19763908682674985 56-57 0.16873268977385786 58-59 0.18150528382048456 60-61 0.1862109763639786 62-63 0.1929333942832558 64-65 0.17814407486084596 66-67 0.18419425098819545 68-69 0.18822770173976175 70-71 0.18486649278012315 72-73 0.18419425098819545 74-75 0.18553873457205086 76-77 0.19158891069940034 78-79 0.19965581220253303 80-81 0.19427787786711123 82-83 0.19562236145096668 84-85 0.1868832181559063 86-87 0.1895721853236172 88-89 0.1862109763639786 90-91 0.1862109763639786 92-93 0.19158891069940034 94-95 0.1929333942832558 96-97 0.17411062410927963 98-99 0.17679959127699052 100-101 0.17411062410927963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74378.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.25235956868967 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24485125858124 26.17306192691387 2 9.492753623188406 6.692839280432386 3 4.164759725400458 4.404528220710425 4 2.3836765827612507 3.3612089596386028 5 1.6323417238749047 2.877194869450644 6 1.0373760488176964 2.19419720885208 7 0.8962623951182305 2.2116754954422007 8 0.7437070938215103 2.097394390814488 9 0.6025934401220443 1.9118556562424374 >10 4.40884820747521 29.8623248810132 >50 0.2631578947368421 6.312350426201297 >100 0.11060259344012205 6.253193148511657 >500 0.011441647597254006 2.6916561348785932 >1k 0.007627765064836004 2.956519400898115 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1134 1.5246443840920703 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 1.4318750168060448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 1.1495334641964021 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.7838339293877221 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 564 0.7582887412944688 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 433 0.582161391809406 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 301 0.40468955874048784 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 271 0.36435505122482453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 224 0.3011643227836188 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 220 0.29578638844819705 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 186 0.250073946597112 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 176 0.23662911075855764 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 166 0.22318427492000323 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 160 0.2151173734168706 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 158 0.2124284062491597 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 158 0.2124284062491597 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 147 0.19763908682674985 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 146 0.1962946032428944 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 139 0.1868832181559063 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 138 0.18553873457205086 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.17747183306891823 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.170749415149641 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 127 0.170749415149641 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 123 0.16537148081421926 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 122 0.16402699723036382 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 121 0.16268251364650838 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 118 0.15864906289494207 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 115 0.15461561214337574 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 111 0.14923767780795397 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 110 0.14789319422409852 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 108 0.14520422705638764 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 107 0.1438597434725322 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 104 0.1398262927209659 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 103 0.13848180913711045 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 100 0.13444835838554411 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.13310387480168867 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 98 0.13175939121783323 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.1277259404662669 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 94 0.12638145688241145 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 93 0.125036973298556 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 91 0.12234800613084515 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 90 0.1210035225469897 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 87 0.11697007179542337 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 86 0.11562558821156795 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 83 0.11159213746000161 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 82 0.11024765387614617 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 82 0.11024765387614617 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 82 0.11024765387614617 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 78 0.10486971954072441 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 75 0.10083626878915808 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 75 0.10083626878915808 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.001344483583855441 0.0 14-15 0.0 0.0 0.0 0.001344483583855441 0.0 16-17 0.0 0.0 0.0 0.001344483583855441 0.0 18-19 0.0 0.0 0.0 0.001344483583855441 0.0 20-21 0.0 0.0 0.0 0.002688967167710882 0.0 22-23 0.0 0.0 0.0 0.002688967167710882 0.0 24-25 0.0 0.0 0.0 0.006722417919277205 0.0 26-27 0.0 0.0 0.0 0.012100352254698968 0.0 28-29 0.0 0.0 0.0 0.02554518809325338 0.0 30-31 0.0 0.0 0.0 0.049073650810723596 0.0 32-33 0.0 0.0 0.0 0.08268574040710963 0.0 34-35 0.0 0.0 0.0 0.1176423135873511 0.0 36-37 0.0 0.0 0.0 0.16738820619000244 0.0 38-39 0.0 0.0 0.0 0.26015757347602786 0.0 40-41 0.0 0.0 0.0 0.336793137755788 0.0 42-43 0.0 0.0 0.0 0.39326144827771653 0.0 44-45 0.0 0.0 0.0 0.428218021457958 0.0 46-47 0.0 0.0 0.0 0.49275323348301914 0.0 48-49 0.0 0.0 0.0 0.5384656753341042 0.0 50-51 0.0 0.0 0.0 0.5761112156820565 0.0 52-53 0.0 0.0 0.0 0.6574524725053107 0.0 54-55 0.0 0.0 0.0 0.7636666756298905 0.0 56-57 0.0 0.0 0.0 0.9209712549409772 0.0 58-59 0.0 0.0 0.0 1.103821022345317 0.0 60-61 0.0 0.0 0.0 1.3357444405603807 0.0 62-63 0.0 0.0 0.0 1.5017881631665277 0.0 64-65 0.0 0.0 0.0 1.6685041275646024 0.0 66-67 0.0 0.0 0.0 1.9232837667052085 0.0 68-69 0.0 0.0 0.0 2.2661270805883458 0.0 70-71 0.0 0.0 0.0 2.647960418403291 0.0 72-73 0.0 0.0 0.0 2.9914759740783565 0.0 74-75 0.0 0.0 0.0 3.3686036193498077 0.0 76-77 0.0 0.0 0.0 3.7343031541584875 0.0 78-79 0.0 0.0 0.0 4.09328027104789 0.0 80-81 0.0 0.0 0.0 4.448896178977655 0.0 82-83 0.0 0.0 0.0 4.842829869047299 0.0 84-85 0.0 0.0 0.0 5.3866734787168244 0.0 86-87 0.0 0.0 0.0 6.005808169082256 0.0 88-89 0.0 0.0 0.0 6.617548199736481 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 20 0.0020614632 71.293015 2 CCTTTAG 25 0.00497754 57.072918 1 TACCTGG 25 0.0049908697 57.03441 2 TGAAGCA 25 0.0050042258 56.995953 4 CATTCCG 25 0.0050042258 56.995953 9 CAGATCA 25 0.0050042258 56.995953 9 AGGCATT 25 0.0050042258 56.995953 6 TAGGCAT 25 0.0050042258 56.995953 5 ATACGTC 25 0.0050042258 56.995953 5 GCAGATC 25 0.0050042258 56.995953 8 AGCAGAT 25 0.0050042258 56.995953 7 TGGGGAA 55 4.9203976E-5 43.178753 6 ACCCTTG 50 0.0016066444 37.997303 7 TTGAAGG 100 1.2783057E-6 33.24764 9 TTAGGCA 60 0.0039287317 31.66442 4 CAATACT 60 0.0039287317 31.66442 4 CCTTGGC 60 0.0039287317 31.66442 9 AGGATTG 110 2.689745E-6 30.225126 5 CTATTAA 95 3.4174373E-5 30.03838 1 GTATAGG 65 0.005773108 29.268167 1 >>END_MODULE