##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74378 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00732743553201 31.0 31.0 34.0 30.0 34.0 2 32.223332168114226 33.0 31.0 34.0 30.0 34.0 3 32.22981257898841 34.0 31.0 34.0 30.0 34.0 4 35.9245341364382 37.0 35.0 37.0 35.0 37.0 5 35.84085347817903 37.0 35.0 37.0 35.0 37.0 6 35.91762349081718 37.0 35.0 37.0 35.0 37.0 7 35.85445965204765 37.0 35.0 37.0 35.0 37.0 8 35.84143160612009 37.0 35.0 37.0 35.0 37.0 9 37.47009868509505 39.0 37.0 39.0 35.0 39.0 10-11 37.39694533329748 39.0 37.0 39.0 34.0 39.0 12-13 37.3333781494528 39.0 37.0 39.0 34.0 39.0 14-15 38.372637070101376 40.0 38.0 41.0 33.5 41.0 16-17 38.3471523837694 40.0 38.0 41.0 33.5 41.0 18-19 38.43817392239641 40.0 38.0 41.0 34.0 41.0 20-21 38.371830379951064 40.0 38.0 41.0 34.0 41.0 22-23 38.30188362150098 40.0 38.0 41.0 34.0 41.0 24-25 38.27817365349969 40.0 37.5 41.0 34.0 41.0 26-27 38.01263142327032 40.0 37.0 41.0 33.5 41.0 28-29 37.896299981177236 40.0 37.0 41.0 33.0 41.0 30-31 37.75007394659711 39.5 36.5 41.0 33.0 41.0 32-33 37.41966710586463 39.0 35.0 41.0 33.0 41.0 34-35 37.181713678775985 39.0 35.0 41.0 33.0 41.0 36-37 36.9082524402377 39.0 35.0 41.0 32.5 41.0 38-39 36.67953561537014 39.0 35.0 41.0 31.5 41.0 40-41 36.432439699911264 39.0 35.0 41.0 31.0 41.0 42-43 36.08267229557127 39.0 35.0 41.0 30.5 41.0 44-45 35.59182150635941 39.0 35.0 40.5 29.5 41.0 46-47 35.00157304579311 38.0 35.0 40.0 25.5 41.0 48-49 34.46904326548173 38.0 35.0 40.0 23.0 41.0 50-51 34.10706795020033 38.0 34.0 40.0 20.0 41.0 52-53 33.83972411196859 38.0 34.0 40.0 16.0 41.0 54-55 33.58910564952002 38.0 34.0 40.0 10.5 41.0 56-57 33.20732609104843 38.0 33.0 40.0 4.5 41.0 58-59 32.93038936244589 38.0 33.0 40.0 2.0 41.0 60-61 32.50006050176127 37.5 33.0 40.0 2.0 41.0 62-63 32.04932910269166 37.0 32.0 40.0 2.0 41.0 64-65 31.603868079270754 36.0 31.5 39.0 2.0 40.5 66-67 31.118153217349217 36.0 31.0 39.0 2.0 40.0 68-69 30.57002070504719 35.0 30.5 38.5 2.0 40.0 70-71 30.096681814515044 35.0 29.5 38.0 2.0 40.0 72-73 29.578020382371133 35.0 29.0 37.0 2.0 39.0 74-75 29.0663973217887 34.5 29.0 37.0 2.0 39.0 76-77 27.748547957729436 32.5 26.5 35.0 2.0 37.5 78-79 28.304969211325933 34.0 28.0 36.0 2.0 38.0 80-81 28.04828712791417 34.0 27.5 35.5 2.0 37.0 82-83 27.62135308827879 34.0 26.5 35.0 2.0 37.0 84-85 27.178439861249295 34.0 26.0 35.0 2.0 36.0 86-87 26.87843179434779 34.0 26.0 35.0 2.0 36.0 88-89 26.44048643416064 33.0 25.0 35.0 2.0 35.5 90-91 25.914282449111298 33.0 23.5 35.0 2.0 35.0 92-93 25.459652047648497 32.5 20.5 35.0 2.0 35.0 94-95 24.94725590900535 32.0 17.5 34.0 2.0 35.0 96-97 24.412534620452284 32.0 4.5 34.0 2.0 35.0 98-99 23.659838930866655 31.5 2.0 34.0 2.0 35.0 100-101 21.375783161687593 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4008766435963089 1101 2 0.5127547581762357 1101 3 0.30221087803212043 1101 4 0.4859067626484972 1101 5 0.6430243244049194 1101 6 0.6189309917102364 1101 7 0.47803232397101425 1101 8 0.49252537561056187 1101 9 0.17356334405396012 1101 10-11 0.2505537733537082 1101 12-13 0.322703711863376 1101 14-15 0.05609248560834601 1101 16-17 0.6051861587086336 1101 18-19 0.6420175287743746 1101 20-21 0.4957902504781444 1101 22-23 0.1556029165289914 1101 24-25 0.11865631269145638 1101 26-27 0.32728356257717195 1101 28-29 0.5248215875005329 1101 30-31 0.26979622207423404 1101 32-33 0.13747868260247031 1101 34-35 1.213170759215764 1101 36-37 0.8905926993677653 1101 38-39 1.1469252975391768 1101 40-41 0.3283435825959202 1101 42-43 0.029084174238874994 1101 44-45 0.7109426251397366 1101 46-47 0.8037625249652649 1101 48-49 1.2353324766084413 1101 50-51 1.9317753242350193 1101 52-53 1.8559008793433023 1101 54-55 2.4626905508653962 1101 56-57 2.469921413137719 1101 58-59 2.4179147061680695 1101 60-61 2.2929741132689614 1101 62-63 2.49405165024411 1101 64-65 2.9339271530530304 1101 66-67 2.7708718598567152 1101 68-69 2.2885383873291687 1101 70-71 1.8688788861396795 1101 72-73 1.8267312446504533 1101 74-75 2.292657028889927 1101 76-77 2.5199114827474958 1101 78-79 1.852502675317833 1101 80-81 2.383438284869566 1101 82-83 2.1064363478946397 1101 84-85 1.4906290601603764 1101 86-87 2.212453566832213 1101 88-89 2.65585818555601 1101 90-91 3.0416632752984007 1101 92-93 3.35654838221809 1101 94-95 2.6978858605983937 1101 96-97 1.9078960123562503 1101 98-99 3.213468160156417 1101 100-101 4.256662188373554 1102 1 0.7260903188099839 1102 2 0.5148915103129852 1102 3 0.5043476301688727 1102 4 0.6027871045288435 1102 5 0.36503287141346874 1102 6 0.43217885495810293 1102 7 0.45901522995391986 1102 8 0.3455168286020154 1102 9 0.792794113284728 1102 10-11 0.16914351694345697 1102 12-13 0.6316780708377365 1102 14-15 0.7595112890271523 1102 16-17 0.9861690646915378 1102 18-19 0.7741756484324895 1102 20-21 1.0526278573157555 1102 22-23 0.9341285575546365 1102 24-25 0.06513067166581976 1102 26-27 0.10217672497033448 1102 28-29 0.7646720148509516 1102 30-31 1.0624244272024441 1102 32-33 1.0072436398674327 1102 34-35 1.4188331523781557 1102 36-37 1.418477314752387 1102 38-39 1.0683996565135345 1102 40-41 1.553664095416437 1102 42-43 1.0818619520166521 1102 44-45 0.9768614285585429 1102 46-47 1.0766257728285211 1102 48-49 1.3626829039588628 1102 50-51 1.2293180592777517 1102 52-53 1.4440419049843243 1102 54-55 1.791750379925226 1102 56-57 2.2431051738214762 1102 58-59 2.715777954031317 1102 60-61 2.8212860790809273 1102 62-63 3.0716157528082064 1102 64-65 3.051662195788065 1102 66-67 2.8718333983182553 1102 68-69 2.3532819770727684 1102 70-71 3.2140711938319875 1102 72-73 2.6994876549068607 1102 74-75 2.711567285300184 1102 76-77 2.4517490895850997 1102 78-79 2.557203530018686 1102 80-81 2.071899823331105 1102 82-83 3.0581457496040407 1102 84-85 2.6442615387928576 1102 86-87 2.91843647281512 1102 88-89 3.2606658778637048 1102 90-91 2.9463641299992567 1102 92-93 2.5609287240984315 1102 94-95 2.61145423666677 1102 96-97 2.568900285860522 1102 98-99 1.8230835447718015 1102 100-101 2.8644613336727005 1103 1 0.234770874365541 1103 2 0.5548220658685423 1103 3 0.3099031857244299 1103 4 0.24167599341772927 1103 5 -0.03774490636430983 1103 6 0.1266232994025458 1103 7 0.373945785509477 1103 8 0.20141960637979395 1103 9 0.13654411328472804 1103 10-11 0.6274768502767856 1103 12-13 0.05094890417107223 1103 14-15 0.499962677916038 1103 16-17 0.6589121202470949 1103 18-19 0.4321617595436038 1103 20-21 0.40071813509353404 1103 22-23 0.3507952242213008 1103 24-25 0.15627650499914836 1103 26-27 0.5743989471925559 1103 28-29 0.7585956259620659 1103 30-31 0.3176327605357727 1103 32-33 0.32582002875631844 1103 34-35 0.7816803746003771 1103 36-37 0.785664814752387 1103 38-39 1.0423579898468702 1103 40-41 1.1448099287497655 1103 42-43 1.1556466742388736 1103 44-45 1.6235628174474286 1103 46-47 1.536695217272964 1103 48-49 1.340981515069977 1103 50-51 0.309179170388866 1103 52-53 0.3763335716509957 1103 54-55 0.941055935480783 1103 56-57 1.0208829515992548 1103 58-59 1.60553489847576 1103 60-61 1.2839596901920416 1103 62-63 1.2070324194748778 1103 64-65 0.9596483068991795 1103 66-67 1.8015208983182553 1103 68-69 0.842865310406097 1103 70-71 0.24532119383198747 1103 72-73 0.3349043215735321 1103 74-75 0.4094839519668483 1103 76-77 0.8310893673628783 1103 78-79 0.7794257522409076 1103 80-81 1.0797123233311048 1103 82-83 1.7838401940484836 1103 84-85 1.6980809832373005 1103 86-87 2.0521170283706773 1103 88-89 2.1669158778637048 1103 90-91 2.473273852221478 1103 92-93 2.887317612987321 1103 94-95 3.0298570144445485 1103 96-97 2.351886396971633 1103 98-99 1.5340210447718015 1103 100-101 1.931301611450479 1104 1 0.2635299323365565 1104 2 0.11030213833230817 1104 3 -0.27705333601469917 1104 4 0.2616035296496122 1104 5 -0.7297738918715595 1104 6 -0.1342462658148449 1104 7 -0.5705741420267572 1104 8 -0.07960394434484641 1104 9 -0.5731479157007797 1104 10-11 0.1926942415811297 1104 12-13 -0.031365407423130875 1104 14-15 -0.1124693873013527 1104 16-17 -0.37675364062246786 1104 18-19 -0.11561903755784897 1104 20-21 -0.43408711852965354 1104 22-23 -0.525110572880152 1104 24-25 -0.7020794732617262 1104 26-27 -0.11128945860454564 1104 28-29 -0.15410817838576207 1104 30-31 -0.05261180468161797 1104 32-33 0.05464702150994327 1104 34-35 -1.381589552935857 1104 36-37 -0.7780806562621052 1104 38-39 -0.6607670101531298 1104 40-41 -0.026951846612554675 1104 42-43 -1.2375824924277907 1104 44-45 -0.34167721878446145 1104 46-47 0.4540412317657143 1104 48-49 0.3795911165192507 1104 50-51 0.29966830082364737 1104 52-53 0.5192230644046205 1104 54-55 0.6444073847561427 1104 56-57 0.6576583139180983 1104 58-59 0.3522513839830097 1104 60-61 -0.44169701270650563 1104 62-63 -0.8541088848729501 1104 64-65 -1.139762924984879 1104 66-67 -0.603710079942612 1104 68-69 -0.688384689593903 1104 70-71 -0.600240400370911 1104 72-73 0.3761180896894736 1104 74-75 0.5258788795030824 1104 76-77 0.1117732441744721 1104 78-79 0.39899096963221226 1104 80-81 0.5247983740557416 1104 82-83 1.1438945418745696 1104 84-85 0.0470393165706362 1104 86-87 0.37254275300835715 1104 88-89 -0.2692254264841232 1104 90-91 -0.0017035028509830852 1104 92-93 1.0467379028423913 1104 94-95 0.30680447821266554 1104 96-97 0.3113519766817774 1104 98-99 0.0748951389746999 1104 100-101 1.3985796911606236 1105 1 0.23752822730671497 1105 2 0.7910353011626583 1105 3 0.42755024454795887 1105 4 -0.07450047717050268 1105 5 0.4769609759886322 1105 6 0.31779976999077775 1105 7 0.566041373744774 1105 8 0.285059312262149 1105 9 0.660441172108257 1105 10-11 0.8921827326297276 1105 12-13 0.9576584629946012 1105 14-15 0.927811942621922 1105 16-17 1.1373128555412109 1105 18-19 0.49925734777890085 1105 20-21 -0.029888482553523943 1105 22-23 0.4868246359860109 1105 24-25 0.5271404755873803 1105 26-27 0.7545460060160849 1105 28-29 0.06328312596206587 1105 30-31 0.1788459958298887 1105 32-33 0.12775017581514447 1105 34-35 0.3432612569533191 1105 36-37 -0.055787391129968 1105 38-39 -0.14606112780018776 1105 40-41 0.28037978169093947 1105 42-43 0.8187900565918156 1105 44-45 0.39148561156508066 1105 46-47 0.8078349231553119 1105 48-49 0.3487940150699771 1105 50-51 -0.361776711964076 1105 52-53 -0.25326201658429426 1105 54-55 -0.520341123342746 1105 56-57 -0.3798523425183902 1105 58-59 -0.71477760152424 1105 60-61 -1.0345145745138424 1105 62-63 -0.7764234628780642 1105 64-65 -0.47693257545376255 1105 66-67 -0.48478424874056714 1105 68-69 0.591027075111981 1105 70-71 0.4162770761849295 1105 72-73 -0.27722803136764185 1105 74-75 -0.2577954597978547 1105 76-77 -0.5792966620488862 1105 78-79 -0.8749860124649764 1105 80-81 -0.47957076490419226 1105 82-83 -0.06358627653975191 1105 84-85 -0.34511754617446755 1105 86-87 -0.5995373833940292 1105 88-89 -0.33859882801865027 1105 90-91 -0.24317835366087692 1105 92-93 -0.5244470928950342 1105 94-95 -0.4012091620260385 1105 96-97 0.15427610285398785 1105 98-99 0.30102472124238844 1105 100-101 -0.008496182667165897 1106 1 -0.10168745896779186 1106 2 -0.5795529341314563 1106 3 -0.5400968142755715 1106 4 -0.39999067324893645 1106 5 -0.38774490636431125 1106 6 -0.3900433672641199 1106 7 0.03957078550947557 1106 8 -0.4767053936202075 1106 9 -0.37387255338193626 1106 10-11 -0.11418981638988157 1106 12-13 -0.5516552624955935 1106 14-15 -0.9515998220839634 1106 16-17 -1.024942046419575 1106 18-19 -0.26887990712306475 1106 20-21 -0.16959436490646596 1106 22-23 -0.020038109112036295 1106 24-25 -0.0890359950008488 1106 26-27 0.25356561385922305 1106 28-29 0.35494979262873017 1106 30-31 -0.3500755727975644 1106 32-33 -0.2830341379103487 1106 34-35 0.37855537460037425 1106 36-37 0.4643106480857213 1106 38-39 -0.019100343486464055 1106 40-41 0.8661640954164298 1106 42-43 0.8957508409055421 1106 44-45 0.2949169841140886 1106 46-47 0.7960702172729697 1106 48-49 -0.45953931826336003 1106 50-51 -1.0720708296111283 1106 52-53 -1.0184580950156672 1106 54-55 -1.4110273978525498 1106 56-57 -1.6999503817340766 1106 58-59 -0.9064442681909028 1106 60-61 -1.5884361431412906 1106 62-63 -0.7450509138584565 1106 64-65 -0.6788933597674891 1106 66-67 -0.0239999350150768 1106 68-69 -0.26338468959390227 1106 70-71 0.2182378604986539 1106 72-73 -0.20467901175980074 1106 74-75 0.23240061863351613 1106 76-77 0.5378602006962083 1106 78-79 -0.00390758109242384 1106 80-81 0.3885664899977712 1106 82-83 0.69425686071515 1106 84-85 1.1387059832373012 1106 86-87 1.4239920283706802 1106 88-89 1.0731658778637048 1106 90-91 0.32691968555480955 1106 92-93 0.9789842796539894 1106 94-95 1.2017320144445485 1106 96-97 1.3175113969716357 1106 98-99 1.3480835447718 1106 100-101 1.329739111450479 1107 1 0.3605932427865959 1107 2 0.2151839079738025 1107 3 0.5335873962507449 1107 4 0.05088651973351688 1107 5 0.22541298837253265 1107 6 0.3108338257183334 1107 7 0.2711497328778947 1107 8 -0.0346001304623087 1107 9 0.069109902758413 1107 10-11 0.048529481855730694 1107 12-13 0.06369561469738727 1107 14-15 0.3391896516002433 1107 16-17 0.5811983044576223 1107 18-19 0.2109446542804463 1107 20-21 -0.06433120701172612 1107 22-23 0.13697943474761587 1107 24-25 -0.5127202055271667 1107 26-27 0.334267368245186 1107 28-29 0.5172304943831207 1107 30-31 0.9253630236936701 1107 32-33 0.5244220024405237 1107 34-35 0.8285553746003771 1107 36-37 0.882293104226072 1107 38-39 0.8392329898468702 1107 40-41 1.1534447971708204 1107 42-43 1.3961894373967638 1107 44-45 1.4743029490263737 1107 46-47 1.5539649541150666 1107 48-49 1.6843203308594568 1107 50-51 1.8963502230204412 1107 52-53 1.6205769927036258 1107 54-55 2.1894111986386804 1107 56-57 2.0175934779150495 1107 58-59 2.5220645037389176 1107 60-61 1.9554235059815142 1107 62-63 1.7711771563169876 1107 64-65 2.3075101490044396 1107 66-67 1.9738070825287828 1107 68-69 2.015562678827152 1107 70-71 1.7502554043583025 1107 72-73 2.0141806373630047 1107 74-75 1.9538918467036908 1107 76-77 1.9597900252576146 1107 78-79 2.0228468048724864 1107 80-81 2.6903208759626835 1107 82-83 2.2328533519432234 1107 84-85 2.483442825342564 1107 86-87 2.5003078178443623 1107 88-89 2.5007974568110747 1107 90-91 2.4054284574846356 1107 92-93 2.736001823513636 1107 94-95 3.4517320144445485 1107 96-97 3.7754061338137355 1107 98-99 3.2967677552981165 1107 100-101 3.8692127956610065 1108 1 0.01930019535319616 1108 2 0.15378039920187803 1108 3 0.0691624449836894 1108 4 0.15371303045476736 1108 5 0.1628723775863108 1108 6 0.1142776203901974 1108 7 0.04080535341071112 1108 8 -0.061890578805389396 1108 9 -0.2843663805424299 1108 10-11 0.10895833175826652 1108 12-13 0.36933239182538813 1108 14-15 0.2780298075456713 1108 16-17 0.29018141037055045 1108 18-19 0.17772503114854032 1108 20-21 0.2779364992910658 1108 22-23 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68-69 -1.212481075136072 2316 70-71 -0.9342721796619884 2316 72-73 -1.3047794133662265 2316 74-75 -1.3288443612861656 2316 76-77 -1.564147831432301 2316 78-79 -3.5488875007711407 2316 80-81 -2.370871261006247 2316 82-83 -2.542590528843082 2316 84-85 -2.9308723300157133 2316 86-87 -2.375405561990771 2316 88-89 -1.7385811100881021 2316 90-91 -2.117558226091777 2316 92-93 -2.895814917133162 2316 94-95 -2.5241716000132826 2316 96-97 -3.236404265678967 2316 98-99 -2.6211935636619366 2316 100-101 -3.179297033127831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 3.0 6 11.0 7 26.0 8 50.0 9 67.0 10 130.0 11 190.0 12 339.0 13 450.0 14 608.0 15 872.0 16 1176.0 17 1612.0 18 1351.0 19 815.0 20 754.0 21 756.0 22 796.0 23 752.0 24 830.0 25 950.0 26 969.0 27 1063.0 28 1269.0 29 1470.0 30 1823.0 31 2203.0 32 2636.0 33 3434.0 34 4648.0 35 6854.0 36 9113.0 37 14502.0 38 11158.0 39 697.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.11809329100984 24.367837736052078 17.41539785871846 18.098671114219616 2 14.664282449111296 25.402672833364704 39.289843771007554 20.643200946516444 3 17.720293635214716 29.84619107800694 33.80703971604507 18.62647557073328 4 11.071822313049557 21.874747909328025 40.087122536233835 26.96630724138858 5 9.54417103670835 41.65523732687912 36.08578727981713 12.714804356595403 6 24.21818279598806 42.387533948210496 17.585845276829172 15.808437978972275 7 21.812901664470676 38.799107262900314 23.250154615612143 16.13783645701686 8 23.29317809029552 35.506466966038346 21.496948022264647 19.703406921401488 9 23.968108849390948 18.846970878485575 24.039366479335285 33.14555379278819 10-11 21.85592513915405 29.80451208690742 28.207937831079082 20.131624942859446 12-13 24.067363608860802 29.214427375710105 28.867524958822145 17.850684056606944 14-15 21.70534297776224 30.788674070289602 29.012611256016562 18.493371695931593 16-17 16.331442093092043 33.74048777864422 30.799429938960444 19.12864018930329 18-19 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30.708147869236047 34.9770719309334 18.081572825195323 44-45 16.831589986286268 30.25155287853935 35.0009411385087 17.915915996665678 46-47 18.053725564010865 30.620613622307673 34.17072252547796 17.1549382882035 48-49 17.329721154104707 30.401462798139235 34.67557611121568 17.593239936540375 50-51 16.68033558310253 30.7812794105784 34.4019737019011 18.136411304417972 52-53 17.072252547796392 32.119040576514564 33.30285837209927 17.50584850358977 54-55 17.608701497754712 30.61254672080454 32.96068730000807 18.818064481432682 56-57 18.03086934308532 31.265965742558283 32.12374626905806 18.57941864529834 58-59 18.085320928231464 30.232730108365374 33.01513888515421 18.666810078248943 60-61 17.731049503885558 31.630320793783106 32.92573072682782 17.71289897550351 62-63 17.407701201968322 31.559735405630697 33.46217967678615 17.57038371561483 64-65 17.413751378095675 31.527467799618165 32.84371722821264 18.215063594073516 66-67 17.479631073704592 31.431337223372502 31.546962811584073 19.542068891338836 68-69 17.04267390895157 30.513727177391164 33.09379117480976 19.349807738847506 70-71 18.25472585979725 30.456586625077307 32.85245637150771 18.436231143617736 72-73 17.658447390357363 29.338648525101508 33.56973836349459 19.433165721046546 74-75 17.0870418672188 31.08647718411358 32.17685337062034 19.649627578047273 76-77 17.24905213907338 29.83005727500067 33.4319287961494 19.488961789776546 78-79 16.791927720562533 29.67006372852187 33.46890209470542 20.06910645621017 80-81 18.114227325284357 30.07206432009465 31.861571970206242 19.952136384414747 82-83 17.729705020301704 29.84619107800694 32.52373013525505 19.90037376643631 84-85 16.707897496571565 29.688214256903922 32.582887412944686 21.021000833579823 86-87 17.159643980746996 29.998117722982602 32.27903412299336 20.563204173277043 88-89 17.739116405388692 29.362849229610905 31.95098012853263 20.94705423646777 90-91 17.61787267801077 28.022535817293146 32.6897089571806 21.669882547515478 92-93 18.537988894416284 29.33769848205763 32.21627653710152 19.908036086424566 94-95 17.648363763478446 28.049288768184137 32.90623571486192 21.39611175347549 96-97 17.342493748151337 28.59514910322945 32.52171340987927 21.540643738739952 98-99 17.604786393734663 28.874995798460557 31.596248865584347 21.92396894222043 100-101 17.263009277934653 28.43619739142127 32.451929541481775 21.848863789162294 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 381.5 2 187.5 3 204.0 4 177.5 5 157.0 6 225.0 7 406.0 8 541.0 9 573.0 10 479.0 11 322.5 12 280.0 13 289.5 14 286.5 15 280.0 16 290.5 17 292.5 18 283.0 19 272.0 20 278.0 21 326.0 22 381.5 23 390.5 24 424.0 25 517.0 26 726.0 27 967.5 28 990.0 29 1033.5 30 1321.5 31 1626.5 32 2080.5 33 2705.0 34 3147.5 35 3581.5 36 4193.0 37 4594.0 38 4337.5 39 3704.0 40 3201.0 41 2752.5 42 2128.5 43 1970.5 44 2169.0 45 2051.0 46 1787.0 47 2090.5 48 3232.0 49 2991.0 50 1660.5 51 941.0 52 562.5 53 415.5 54 356.0 55 347.0 56 304.5 57 267.0 58 234.5 59 209.0 60 181.5 61 158.5 62 152.0 63 113.0 64 79.0 65 64.0 66 45.5 67 45.0 68 51.5 69 48.0 70 38.0 71 35.5 72 29.0 73 28.0 74 30.5 75 23.0 76 15.0 77 9.5 78 7.0 79 5.0 80 1.5 81 0.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04033450751566323 2 0.0 3 0.0 4 0.0 5 0.010755868670843528 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007394659711204926 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001344483583855441 24-25 0.0 26-27 0.0 28-29 0.001344483583855441 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001344483583855441 38-39 0.0 40-41 0.0 42-43 0.020167253757831616 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010083626878915808 92-93 0.001344483583855441 94-95 0.0 96-97 0.0 98-99 6.722417919277205E-4 100-101 0.010755868670843528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 74378.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.65780203823711 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13253974594551 24.614805453225415 2 9.578972597267715 6.448143268170696 3 4.362067588080211 4.404528220710425 4 2.6284253415355114 3.538680792707521 5 1.7815770552049213 2.998198391997634 6 1.2183430534473116 2.4604049584554573 7 0.8748102580490533 2.061093334050391 8 0.7989134776703682 2.151173734168706 9 0.5632340017576096 1.7061496679125547 >10 4.6257090357114325 29.74804377638549 >50 0.3075816889030918 6.891822850842991 >100 0.10785331948549971 6.476377423431661 >500 0.007989134776703682 1.9616015488450884 >1k 0.011983702165055525 4.538976579095969 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 1.5932130468686978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 1.566323375191589 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1026 1.3794401570356825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 1.1132324074323052 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 631 0.8483691414127834 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.5714055231385625 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 376 0.5055258275296458 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.3858667885665116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 269 0.3616660840571137 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 251 0.3374653795477157 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 217 0.29175293769663074 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 203 0.2729301675226546 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 200 0.26889671677108823 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 193 0.2594853316841001 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 186 0.250073946597112 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.240662561510124 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 176 0.23662911075855764 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 175 0.2352846271747022 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 142 0.19091666890747264 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 140 0.18822770173976175 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.18284976740434 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 127 0.170749415149641 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 125 0.16806044798193012 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 121 0.16268251364650838 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 120 0.16133803006265293 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 115 0.15461561214337574 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 114 0.1532711285595203 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 111 0.14923767780795397 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 110 0.14789319422409852 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 109 0.14654871064024308 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 106 0.14251525988867675 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 104 0.1398262927209659 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 99 0.13310387480168867 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 99 0.13310387480168867 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 97 0.13041490763397778 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 96 0.12907042405012234 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 93 0.125036973298556 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 92 0.1236924897147006 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 89 0.11965903896313426 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 87 0.11697007179542337 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 86 0.11562558821156795 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 86 0.11562558821156795 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 85 0.1142811046277125 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 84 0.11293662104385706 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 84 0.11293662104385706 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 84 0.11293662104385706 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.11159213746000161 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 82 0.11024765387614617 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 82 0.11024765387614617 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 81 0.10890317029229073 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 80 0.1075586867084353 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 79 0.10621420312457985 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 77 0.10352523595686897 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 76 0.10218075237301352 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.001344483583855441 0.0 14-15 0.0 0.0 0.0 0.001344483583855441 0.0 16-17 0.0 0.0 0.0 0.001344483583855441 0.0 18-19 0.0 0.0 0.0 0.001344483583855441 0.0 20-21 0.0 0.0 0.0 0.002688967167710882 0.0 22-23 0.0 0.0 0.0 0.002688967167710882 0.0 24-25 0.0 0.0 0.0 0.006722417919277205 0.0 26-27 0.0 0.0 0.0 0.012100352254698968 0.0 28-29 0.0 0.0 0.0 0.02554518809325338 0.0 30-31 0.0 0.0 0.0 0.049073650810723596 0.0 32-33 0.0 0.0 0.0 0.08134125682325419 0.0 34-35 0.0 0.0 0.0 0.11629783000349565 0.0 36-37 0.0 0.0 0.0 0.1694049315657856 0.0 38-39 0.0 0.0 0.0 0.26419102422759416 0.0 40-41 0.0 0.0 0.0 0.3414988302992821 0.0 42-43 0.0 0.0 0.0 0.4006561079889215 0.0 44-45 0.0 0.0 0.0 0.4376294065449461 0.0 46-47 0.0 0.0 0.0 0.502836860361935 0.0 48-49 0.0 0.0 0.0 0.5519105111726585 0.0 50-51 0.0 0.0 0.0 0.5902282933125387 0.0 52-53 0.0 0.0 0.0 0.6749307590954314 0.0 54-55 0.0 0.0 0.0 0.7851784129715776 0.0 56-57 0.0 0.0 0.0 0.9512221355777246 0.0 58-59 0.0 0.0 0.0 1.1374331119417032 0.0 60-61 0.0 0.0 0.0 1.37137325553255 0.0 62-63 0.0 0.0 0.0 1.5401059453064079 0.0 64-65 0.0 0.0 0.0 1.710183118664121 0.0 66-67 0.0 0.0 0.0 1.968323966764366 0.0 68-69 0.0 0.0 0.0 2.317217456774853 0.0 70-71 0.0 0.0 0.0 2.701739761757509 0.0 72-73 0.0 0.0 0.0 3.0513054935599238 0.0 74-75 0.0 0.0 0.0 3.4338110731667966 0.0 76-77 0.0 0.0 0.0 3.804216300518971 0.0 78-79 0.0 0.0 0.0 4.165882384576085 0.0 80-81 0.0 0.0 0.0 4.528220710425126 0.0 82-83 0.0 0.0 0.0 4.929549060205975 0.0 84-85 0.0 0.0 0.0 5.478770604210922 0.0 86-87 0.0 0.0 0.0 6.101938745327919 0.0 88-89 0.0 0.0 0.0 6.7271236118207 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGGG 40 5.358896E-4 47.493946 5 TGGGGGA 40 5.358896E-4 47.493946 6 CACCTAC 65 1.3172584E-4 36.53381 7 GCAGATC 55 0.0025658049 34.54105 8 AGCAGAT 55 0.0025658049 34.54105 7 ATGGGAT 70 2.0365058E-4 33.92425 5 CAGATCA 60 0.003929886 31.66263 9 CCTGGTC 105 1.8720984E-6 31.66263 8 CCTATAC 75 3.0533198E-4 31.66263 3 CTATACG 65 0.0058130594 29.227047 4 AAGCAGA 65 0.0058130594 29.227047 6 ATACGTC 65 0.0058130594 29.227047 6 TATACGT 65 0.0058130594 29.227047 5 CATTTAC 85 6.3302915E-4 27.956417 1 TCCAGAA 85 6.355284E-4 27.937616 2 TCTGAGC 70 0.008347253 27.1394 3 CATGGGG 105 6.826706E-5 27.139397 4 TTCCAGA 90 8.840507E-4 26.403282 1 CATGGGC 90 8.875356E-4 26.385527 4 CCAGAAG 90 8.875356E-4 26.385527 3 >>END_MODULE