##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937664_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72166 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96323753568162 31.0 31.0 34.0 30.0 34.0 2 32.17239420225591 33.0 31.0 34.0 30.0 34.0 3 32.245544993487236 34.0 31.0 34.0 30.0 34.0 4 35.95143142199928 37.0 35.0 37.0 35.0 37.0 5 35.780450627719425 37.0 35.0 37.0 35.0 37.0 6 35.91974059806557 37.0 35.0 37.0 35.0 37.0 7 35.85508411163152 37.0 35.0 37.0 35.0 37.0 8 35.879361472161406 37.0 35.0 37.0 35.0 37.0 9 37.48094670620514 39.0 37.0 39.0 35.0 39.0 10-11 37.43477537898734 39.0 37.0 39.0 34.0 39.0 12-13 37.365885597095584 39.0 37.0 39.0 34.0 39.0 14-15 38.401109940969434 40.0 38.0 41.0 34.0 41.0 16-17 38.3813360862456 40.0 38.0 41.0 33.5 41.0 18-19 38.481189202671615 40.0 38.0 41.0 34.0 41.0 20-21 38.47434387384641 40.0 38.0 41.0 34.0 41.0 22-23 38.32188288113516 40.0 37.5 41.0 34.0 41.0 24-25 38.21813596430452 40.0 37.0 41.0 34.0 41.0 26-27 38.02737438683036 40.0 37.0 41.0 33.5 41.0 28-29 37.872162791342184 40.0 37.0 41.0 33.0 41.0 30-31 37.769746140841946 39.5 36.5 41.0 33.0 41.0 32-33 37.496251697475266 39.0 35.5 41.0 33.0 41.0 34-35 37.24694454452235 39.0 35.0 41.0 33.0 41.0 36-37 37.00196075714325 39.0 35.0 41.0 33.0 41.0 38-39 36.76249203225896 39.0 35.0 41.0 32.0 41.0 40-41 36.52734667294848 39.0 35.0 41.0 31.0 41.0 42-43 36.20543607793144 39.0 35.0 41.0 30.5 41.0 44-45 35.733427098633705 39.0 35.0 40.5 29.5 41.0 46-47 35.1467172906909 38.5 35.0 40.0 26.0 41.0 48-49 34.63601280381343 38.0 35.0 40.0 23.5 41.0 50-51 34.28937449768589 38.0 34.5 40.0 22.0 41.0 52-53 34.03933985533354 38.0 34.0 40.0 19.5 41.0 54-55 33.79932378128204 38.0 34.0 40.0 16.0 41.0 56-57 33.414821384031264 38.0 33.5 40.0 7.5 41.0 58-59 33.08362663858327 38.0 33.0 40.0 2.0 41.0 60-61 32.68641049801846 37.5 33.0 40.0 2.0 41.0 62-63 32.28035362913283 37.0 32.5 40.0 2.0 41.0 64-65 31.806584818335505 36.0 31.5 39.0 2.0 40.5 66-67 31.31244630435385 36.0 31.0 39.0 2.0 40.0 68-69 30.809016711470775 35.0 31.0 38.5 2.0 40.0 70-71 30.31527312030596 35.0 30.0 38.0 2.0 40.0 72-73 29.829192417481917 35.0 29.5 37.0 2.0 39.5 74-75 29.31218995094643 34.5 29.0 37.0 2.0 39.0 76-77 28.02987556467034 33.5 27.0 35.5 2.0 37.5 78-79 28.572651941357428 34.0 29.0 36.0 2.0 38.0 80-81 28.284476069062993 34.0 28.5 35.5 2.0 37.0 82-83 27.88852091012388 34.0 27.5 35.0 2.0 37.0 84-85 27.46641077515728 34.0 26.5 35.0 2.0 36.0 86-87 27.08204694731591 34.0 26.0 35.0 2.0 36.0 88-89 26.55903749688219 33.0 25.5 35.0 2.0 35.5 90-91 26.00692847047086 33.0 24.0 35.0 2.0 35.0 92-93 25.622433001690545 32.5 21.5 35.0 2.0 35.0 94-95 25.124102763074024 32.0 19.0 34.5 2.0 35.0 96-97 24.55057783443727 32.0 8.5 34.0 2.0 35.0 98-99 23.757870742454898 31.5 2.0 34.0 2.0 35.0 100-101 21.511161765928556 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3610706389328442 1101 2 -0.20873078925306743 1101 3 -0.5907124354384123 1101 4 0.06307091823047983 1101 5 0.29795500764591054 1101 6 0.3537472648629745 1101 7 -0.1566643424634293 1101 8 -0.37048297131144636 1101 9 -0.06692358327867254 1101 10-11 0.4344236566000177 1101 12-13 0.13808522020358538 1101 14-15 0.2027645872654702 1101 16-17 -0.060146678327782865 1101 18-19 0.5658618275236833 1101 20-21 0.3042314486299844 1101 22-23 0.3055001835883928 1101 24-25 0.3227241427151668 1101 26-27 -0.6891410901251405 1101 28-29 -0.23580500479425126 1101 30-31 0.5269325461772567 1101 32-33 0.30864864817510096 1101 34-35 0.563564530391119 1101 36-37 0.628993262018497 1101 38-39 0.9613935169069734 1101 40-41 0.7460522105930281 1101 42-43 1.5455132887146306 1101 44-45 0.4345303870289712 1101 46-47 0.020805934224483735 1101 48-49 -0.152174305583344 1101 50-51 -0.38240622508268274 1101 52-53 -0.6194712352006917 1101 54-55 -0.02988975681095951 1101 56-57 -0.7505501397292988 1101 58-59 -0.8360076153077642 1101 60-61 -0.5505058890625847 1101 62-63 -0.10733964121838113 1101 64-65 0.309738483495547 1101 66-67 -0.05980688601428952 1101 68-69 -0.781235283536919 1101 70-71 -0.499627021651051 1101 72-73 -0.03906705980019254 1101 74-75 -0.304820794327501 1101 76-77 0.10219090329160707 1101 78-79 -0.50648333623786 1101 80-81 0.2232930813898122 1101 82-83 0.1341863512388457 1101 84-85 0.31266529190098424 1101 86-87 0.2717855759689307 1101 88-89 -0.11760921651162803 1101 90-91 -0.991149738174542 1101 92-93 -0.1974521298402685 1101 94-95 0.24483787297607051 1101 96-97 -0.2447638208658276 1101 98-99 -0.45402489931199597 1101 100-101 -0.10237642201710173 1102 1 0.06763172490569502 1102 2 -0.19470363993179873 1102 3 0.23847308492357655 1102 4 0.07777680058342185 1102 5 0.16288713434274626 1102 6 -0.07951065368906285 1102 7 -0.04851954608334097 1102 8 0.28472064859805357 1102 9 0.2778727968118275 1102 10-11 0.16112048917920418 1102 12-13 -0.05184690649324608 1102 14-15 0.022221600840126143 1102 16-17 0.45614291443239807 1102 18-19 0.045499836573455354 1102 20-21 -0.5045920807817836 1102 22-23 -0.17368533677359466 1102 24-25 0.23347074905001364 1102 26-27 -0.942082266595726 1102 28-29 -0.5129543260612124 1102 30-31 -0.7584746936417446 1102 32-33 -0.3152201301054376 1102 34-35 -0.8291956506043618 1102 36-37 -0.16693433979146022 1102 38-39 0.12202700106986697 1102 40-41 0.27161782145275737 1102 42-43 0.10784360545670779 1102 44-45 0.14799192549051554 1102 46-47 -0.8710492693954279 1102 48-49 -0.7080566585245123 1102 50-51 0.2087702455055478 1102 52-53 -0.0018241763771627006 1102 54-55 -0.044482516991962484 1102 56-57 0.095716828596494 1102 58-59 0.9163679503030906 1102 60-61 0.3918018032451087 1102 62-63 0.37444768909836057 1102 64-65 0.06675205815617957 1102 66-67 0.7106682271078846 1102 68-69 -0.28745700299393206 1102 70-71 0.9909838380774545 1102 72-73 1.290684071421527 1102 74-75 0.7197267169847201 1102 76-77 0.7241366046490718 1102 78-79 1.3467972067485654 1102 80-81 1.04512566057533 1102 82-83 1.0626931385691627 1102 84-85 1.0009005860186342 1102 86-87 1.1393195126205136 1102 88-89 0.9868025481942553 1102 90-91 1.4949362346761355 1102 92-93 1.9724573724221734 1102 94-95 1.76486049741046 1102 96-97 1.8641728307178838 1102 98-99 2.709776005665379 1102 100-101 2.0615376051322194 1103 1 0.6583077155816852 1103 2 0.3812870360588789 1103 3 0.7163285627790543 1103 4 0.6686858914925153 1103 5 0.4820013534569654 1103 6 0.6197900456116372 1103 7 0.24262264505885156 1103 8 0.5394992033766073 1103 9 0.659457878396907 1103 10-11 0.7726589507176627 1103 12-13 0.7305540259076864 1103 14-15 1.1448323234508493 1103 16-17 0.6247909330804191 1103 18-19 0.6661292072028218 1103 20-21 0.24540791921821636 1103 22-23 0.3620955490072859 1103 24-25 0.36260827818754393 1103 26-27 -0.004203478716938491 1103 28-29 0.041824228717338485 1103 30-31 0.45318031801326697 1103 32-33 0.35610854122322877 1103 34-35 0.7324593610506511 1103 36-37 0.7831822103250943 1103 38-39 0.5491831782260448 1103 40-41 0.9421306419655835 1103 42-43 0.4601746077877067 1103 44-45 -0.25865143115284184 1103 46-47 0.003426255080093199 1103 48-49 0.48285243238457554 1103 50-51 0.8845278212631271 1103 52-53 0.793630369077384 1103 54-55 1.1347715622621166 1103 56-57 0.2773019101815777 1103 58-59 0.8700975540326965 1103 60-61 1.207069868513173 1103 62-63 0.9615106261612993 1103 64-65 1.4608080022121293 1103 66-67 0.5571717236113791 1103 68-69 0.9596292440923122 1103 70-71 1.1128952599888784 1103 72-73 1.1875372182746737 1103 74-75 1.2087710060290071 1103 76-77 1.2630643435768114 1103 78-79 0.763347323298678 1103 80-81 0.26540538085505005 1103 82-83 0.2968423227183479 1103 84-85 -0.038493353375304906 1103 86-87 0.41461088791189127 1103 88-89 0.7034692148609203 1103 90-91 0.9391087288486304 1103 92-93 1.3257207756855784 1103 94-95 1.3791961617461261 1103 96-97 1.8797905463355988 1103 98-99 1.8075615534509275 1103 100-101 1.3461529897476048 1104 1 0.08165887422696017 1104 2 0.3059750930998746 1104 3 -0.09071243543841234 1104 4 -0.17222319941657815 1104 5 -0.09910381588349537 1104 6 -0.02860567631349653 1104 7 -0.20078198952225534 1104 8 0.07069349927678559 1104 9 -0.09633534798455656 1104 10-11 -0.4479292845764533 1104 12-13 -0.28838536803170456 1104 14-15 0.09982341079487611 1104 16-17 0.1457356746133982 1104 18-19 -0.015020525417497765 1104 20-21 -0.004592080781783636 1104 22-23 0.08491194829426973 1104 24-25 0.02125355447987687 1104 26-27 -0.17443520777219845 1104 28-29 0.41860675991163276 1104 30-31 0.07105019323608275 1104 32-33 -0.25017488123666 1104 34-35 -0.22320017549124316 1104 36-37 -0.4298302673932639 1104 38-39 -1.0386064830930337 1104 40-41 -0.7539477894069719 1104 42-43 -0.7780161230500795 1104 44-45 0.11835391644073923 1104 46-47 -0.33213524224610325 1104 48-49 -0.5859978349951049 1104 50-51 -0.5515238721415088 1104 52-53 -0.45770652931833666 1104 54-55 -0.3681250509286116 1104 56-57 -0.36819719855283495 1104 58-59 0.2007570905745908 1104 60-61 -0.13874118318023676 1104 62-63 -0.8352808176889681 1104 64-65 0.08179730702496002 1104 66-67 0.13872252575042054 1104 68-69 0.28494118705131655 1104 70-71 0.16213768423130048 1104 72-73 0.005050587258633499 1104 74-75 -0.12835020609220393 1104 76-77 0.043367373879842575 1104 78-79 -0.5947186303555085 1104 80-81 0.3556460225662832 1104 82-83 0.6856569394741427 1104 84-85 0.4670770566068647 1104 86-87 0.6688443994983437 1104 88-89 0.551508430547198 1104 90-91 1.1926737912372225 1104 92-93 1.611371399571496 1104 94-95 1.788955520034893 1104 96-97 1.5199420614871144 1104 98-99 1.413622159511533 1104 100-101 2.5961529897476048 1105 1 0.15369638488339632 1105 2 0.26995633777166006 1105 3 0.4146157828054058 1105 4 0.3531391194240001 1105 5 0.050512552402949495 1105 6 0.42216158711361373 1105 7 0.5001984026346094 1105 8 0.5203951190551308 1105 9 0.33205339881851614 1105 10-11 0.6241082260799757 1105 12-13 0.5648755015335141 1105 14-15 0.6217756699594119 1105 16-17 0.49995562346250466 1105 18-19 0.5070382981119153 1105 20-21 0.9301905279138722 1105 22-23 0.4414763131706607 1105 24-25 0.4739389764747628 1105 26-27 0.4796568638390539 1105 28-29 -0.3033668121258728 1105 30-31 0.20350969877743097 1105 32-33 -0.15245535864502102 1105 34-35 0.20380323456843996 1105 36-37 0.019018837466070693 1105 38-39 0.08245063540654485 1105 40-41 -0.20642008267210343 1105 42-43 -0.10207835663916853 1105 44-45 -0.08177396079711485 1105 46-47 0.20665418656889756 1105 48-49 -0.45588274548103414 1105 50-51 -0.7709398994219896 1105 52-53 -0.38588214739165494 1105 54-55 -0.7064669094111338 1105 56-57 -0.521117062150438 1105 58-59 -0.24617385571697525 1105 60-61 -0.08311458471475675 1105 62-63 -0.024326000979677076 1105 64-65 -0.1719324457457141 1105 66-67 -0.25343433699467965 1105 68-69 -0.00875020254800063 1105 70-71 0.30525362626028496 1105 72-73 0.3742961115553065 1105 74-75 0.7792584895599681 1105 76-77 0.597715199966796 1105 78-79 0.9622933048533575 1105 80-81 1.2864857497615105 1105 82-83 1.1324813214008245 1105 84-85 1.2269875425403747 1105 86-87 1.2215937601121531 1105 88-89 0.6636141423971544 1105 90-91 1.1364078065824899 1105 92-93 1.0776970602705553 1105 94-95 1.615362169651263 1105 96-97 1.8079855397479818 1105 98-99 1.9534772319753024 1105 100-101 2.693979076704128 1106 1 -0.33664177936781314 1106 2 -0.33209680232496197 1106 3 -0.5980653766148833 1106 4 -0.08888986608324245 1106 5 -0.2755744041187924 1106 6 0.10211327793486902 1106 7 -0.4025793751431692 1106 8 -0.20545029157288752 1106 9 0.4624881814272115 1106 10-11 -0.1301188270601088 1106 12-13 0.23244796530162404 1106 14-15 0.35758484870337526 1106 16-17 0.44107881186829445 1106 18-19 0.08342718700080098 1106 20-21 -0.14348096967066937 1106 22-23 -0.5135357641240219 1106 24-25 -0.5073412167619509 1106 26-27 -0.14624893326239885 1106 28-29 -0.1475697106765992 1106 30-31 -0.43507725774430384 1106 32-33 -0.7158611557464596 1106 34-35 -0.4682982147069268 1106 36-37 -0.6120198098769265 1106 38-39 -0.6231653066224396 1106 40-41 -0.8078693580344236 1106 42-43 -0.3605324629193589 1106 44-45 -0.4890807240821431 1106 46-47 -0.9403868762330347 1106 48-49 0.05166556369771058 1106 50-51 0.3504369121722206 1106 52-53 -0.1059908430438341 1106 54-55 0.0015644915550510063 1106 56-57 -0.6349455645659035 1106 58-59 -1.1513670924319506 1106 60-61 -0.6963392223959204 1106 62-63 -1.0460651314144584 1106 64-65 -1.2797223008181753 1106 66-67 -1.197878781439126 1106 68-69 -1.9103202508571826 1106 70-71 -2.546195649102035 1106 72-73 -1.801730458493001 1106 74-75 -1.4686279838699825 1106 76-77 -1.7482992927868217 1106 78-79 -1.5497839898326298 1106 80-81 -1.284468356518687 1106 82-83 -1.0251273742513511 1106 84-85 -1.6615994139813672 1106 86-87 -2.198394162593157 1106 88-89 -1.5187530073613011 1106 90-91 -1.1271791499392485 1106 92-93 -1.371880234415432 1106 94-95 -1.6125967675468047 1106 96-97 -1.0633912718462213 1106 98-99 -1.2599889515995812 1106 100-101 -0.5496803435857309 1107 1 -0.4546022055474168 1107 2 -0.08342861358828202 1107 3 0.2563866781796378 1107 4 0.1325713211313655 1107 5 -0.15913604795441216 1107 6 -0.4188151847744166 1107 7 -0.25779246494530383 1107 8 -0.21496322916801347 1107 9 -0.0032652432303237333 1107 10-11 0.05005621099163449 1107 12-13 -0.09146755981252142 1107 14-15 0.10767997807932517 1107 16-17 0.3266381725988907 1107 18-19 0.5823807638653378 1107 20-21 -0.1826742725626076 1107 22-23 -0.02447564235842492 1107 24-25 0.078465480346118 1107 26-27 -0.13112336248613587 1107 28-29 -0.02770669697796535 1107 30-31 -0.19335351345207386 1107 32-33 0.06781464334029152 1107 34-35 -0.027088169044823474 1107 36-37 -0.6852694293594226 1107 38-39 -0.2678570874443551 1107 40-41 0.38162835886056 1107 42-43 0.3753883894398484 1107 44-45 0.4465166883988303 1107 46-47 -0.24470574990122174 1107 48-49 -0.36148131605876443 1107 50-51 -0.9720516723026762 1107 52-53 -1.4744269161031909 1107 54-55 -1.3438502725241008 1107 56-57 -0.8100787456922376 1107 58-59 -0.8909028610773078 1107 60-61 -0.9648894507064192 1107 62-63 -0.8930970948847801 1107 64-65 -0.2680213875761659 1107 66-67 0.05935105113317363 1107 68-69 -0.1783948322879283 1107 70-71 0.24946645135458922 1107 72-73 0.4487451883867557 1107 74-75 0.7774717117160144 1107 76-77 1.0056961410031313 1107 78-79 0.6815706524809144 1107 80-81 1.3712584319135814 1107 82-83 -0.16154289936550725 1107 84-85 -0.35937338658410667 1107 86-87 -0.36953190992953466 1107 88-89 -0.021416629887934135 1107 90-91 -0.21022709514472737 1107 92-93 0.1919515768478881 1107 94-95 1.5164937956191018 1107 96-97 1.559325623130949 1107 98-99 1.4307454471827654 1107 100-101 1.9557420308434956 1108 1 0.14684170411567266 1108 2 0.5368971916690342 1108 3 0.4255832720337622 1108 4 0.18588490869152707 1108 5 0.07577694723256201 1108 6 0.33109225691384836 1108 7 0.004702907139112256 1108 8 0.12713229100969414 1108 9 -0.06453884559981304 1108 10-11 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-0.5891182616196744 2316 46-47 -0.26377276211892564 2316 48-49 -0.4931917936596548 2316 50-51 0.22768916442446852 2316 52-53 -0.19101336556634863 2316 54-55 -0.37587544838488895 2316 56-57 0.03303491591457686 2316 58-59 -0.03612685219170686 2316 60-61 0.010304921748222284 2316 62-63 0.37060153525220585 2316 64-65 0.3312011226052469 2316 66-67 -0.7579388414991861 2316 68-69 -0.574359289896222 2316 70-71 -0.037186640093022305 2316 72-73 -0.04403526329780405 2316 74-75 0.7939470912050943 2316 76-77 0.4149889955014636 2316 78-79 0.18369949365085247 2316 80-81 -0.11235874690907366 2316 82-83 -0.03751476163873946 2316 84-85 0.1846843698024152 2316 86-87 0.20719893049993132 2316 88-89 -0.44968393829223174 2316 90-91 -0.41940887966897833 2316 92-93 -0.7797255797607754 2316 94-95 -0.9119586294086659 2316 96-97 -0.05776064121558733 2316 98-99 -0.16745892156954767 2316 100-101 -0.20114430754969348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 5.0 6 4.0 7 20.0 8 41.0 9 59.0 10 132.0 11 178.0 12 278.0 13 404.0 14 564.0 15 801.0 16 1128.0 17 1513.0 18 1283.0 19 819.0 20 633.0 21 715.0 22 707.0 23 772.0 24 842.0 25 776.0 26 886.0 27 972.0 28 1186.0 29 1397.0 30 1758.0 31 2168.0 32 2637.0 33 3432.0 34 4679.0 35 6704.0 36 9198.0 37 14090.0 38 10692.0 39 693.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.919880236200825 24.435141803664994 17.293670815890884 18.351307144243297 2 14.648172269489788 25.348502064684205 39.15278663082338 20.850539035002633 3 17.782612310506334 30.16101765374276 33.19984480226145 18.856525233489453 4 10.994096943158828 21.744311725743426 40.36388326912951 26.89770806196824 5 9.458410709831204 41.67937027079465 36.09440394689432 12.767815072479834 6 24.492143114486044 42.58930798436937 17.27267688385112 15.645872017293463 7 22.08796386109802 38.719064379347614 23.217304547848016 15.975667211706343 8 23.398830474184518 35.44466923481972 21.249618934124104 19.906881356871654 9 24.037635451597705 19.078236288556937 24.17620486101488 32.707923398830474 10-11 21.814982124546184 29.709281379042764 28.225203004184795 20.250533492226257 12-13 24.288594649649752 29.21352207140729 28.766620244306335 17.731263034636623 14-15 21.56694288168944 30.85940747720533 29.1037330598897 18.469916581215532 16-17 16.469667156278582 33.78530055704903 30.661946068785856 19.08308621788654 18-19 15.569658842114015 31.684588310284624 35.54790344483551 17.197849402765844 20-21 17.197968558383163 29.733044183162317 35.66662740506197 17.40235985339255 22-23 17.375911093866918 29.547155170024663 35.752986170772935 17.323947565335477 24-25 17.9968267385385 30.384324919802395 35.33475137011453 16.284096971544574 26-27 16.871059377814728 29.833714404489708 37.0324949767893 16.262731240906255 28-29 16.320577577307088 30.56323487635716 36.88291172129955 16.233275825036202 30-31 17.107204966362044 29.967920959461235 35.965246551329926 16.95962752284679 32-33 16.185599866973366 31.398442479838153 36.138209128952695 16.27774852423579 34-35 17.09738658093839 30.296815674971594 36.073774353573704 16.532023390516308 36-37 17.33364742399468 29.931685281157332 35.454369093478924 17.280298201369064 38-39 16.436410498018457 30.85940747720533 35.86730593354211 16.8368760912341 40-41 16.071280104204195 31.026383615553033 36.54906742787462 16.353268852368153 42-43 16.3081890876838 30.79257729672797 35.176074393336755 17.723159222251482 44-45 16.97475265360419 30.33422941551423 34.538425297231385 18.152592633650194 46-47 17.94335282543026 30.927999334866836 33.872599284981845 17.25604855472106 48-49 17.337804506277195 30.86702879472328 34.02987556467034 17.765291134329185 50-51 16.780062633373056 31.37904276251975 33.743036887176785 18.09785771693041 52-53 17.222099049413853 32.437713050466975 32.97051242967603 17.369675470443145 54-55 17.642657206994983 30.828229360086468 32.748108527561456 18.781004905357094 56-57 18.239198514535932 31.434470526286617 31.594518194163456 18.731812765013995 58-59 18.192084915334092 30.636310728043675 32.7751295623978 18.396474794224428 60-61 17.814483274672284 32.06218995094643 32.64487431754566 17.47845245683563 62-63 17.374525399772747 31.967269905495666 33.19360917883768 17.46459551589391 64-65 17.532494526508327 31.645096028600726 32.93102014799213 17.891389296898815 66-67 17.3620541529252 31.78643682620625 31.528697724690296 19.322811296178255 68-69 16.953967242191613 30.659867527644597 32.939334312557165 19.44683091760663 70-71 18.290469196020286 30.45963473103678 32.69406645788875 18.555829615054183 72-73 17.537344455837932 29.51805559404706 33.606546018900865 19.338053931214144 74-75 17.159049968129036 31.602139511681397 31.912534988775874 19.326275531413685 76-77 17.323254718288393 29.852700717789542 33.11808885070532 19.70595571321675 78-79 16.878446914059253 29.98087742150043 33.29407200066513 19.846603663775188 80-81 18.127650139955104 30.66610315106837 31.584125488457172 19.622121220519357 82-83 17.877532355957097 29.985727350830032 32.359421334146276 19.777318959066598 84-85 16.665742870603886 29.88803591719092 32.73425158661974 20.711969625585454 86-87 17.110550674833025 30.226145276168833 32.150181525926335 20.513122523071807 88-89 17.5879222902752 29.417592772219603 31.93262755314137 21.061857384363826 90-91 17.558812320271628 28.280497522780028 32.762360115026155 21.398330041922183 92-93 18.341116638720674 29.602427836980173 32.076687499133904 19.97976802516525 94-95 17.494387938918603 28.51550591691378 32.92894160685087 21.061164537316742 96-97 17.289305212981184 28.94853532134246 32.32755036998032 21.434609095696032 98-99 17.492309397777348 29.331679738380956 31.473962807970512 21.702048055871188 100-101 16.94189390527009 28.60815633553931 32.67800838501784 21.77194137417276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 610.0 1 388.0 2 189.5 3 207.5 4 161.0 5 147.5 6 240.0 7 404.5 8 535.5 9 561.0 10 444.0 11 299.5 12 279.5 13 307.0 14 309.5 15 283.5 16 283.0 17 279.0 18 257.5 19 260.0 20 279.5 21 310.5 22 334.5 23 368.5 24 398.5 25 489.5 26 706.0 27 929.0 28 953.5 29 1024.0 30 1298.0 31 1568.5 32 2078.5 33 2675.5 34 2991.0 35 3397.5 36 3994.0 37 4410.5 38 4140.5 39 3485.0 40 3123.0 41 2716.5 42 2099.0 43 2036.5 44 2174.5 45 1969.5 46 1731.5 47 2013.0 48 3184.5 49 2984.0 50 1628.0 51 906.5 52 518.5 53 401.0 54 343.0 55 320.5 56 285.0 57 225.0 58 220.5 59 217.5 60 187.5 61 159.5 62 139.5 63 97.5 64 71.0 65 64.0 66 47.5 67 47.5 68 44.5 69 41.5 70 28.5 71 30.0 72 37.5 73 28.0 74 19.5 75 10.5 76 6.5 77 6.5 78 7.0 79 9.0 80 6.0 81 2.0 82 1.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033256658260122494 2 0.0 3 0.0 4 0.0 5 0.011085552753374165 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0034642352354294264 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.928470470858853E-4 22-23 0.0 24-25 6.928470470858853E-4 26-27 0.0013856940941717707 28-29 0.0034642352354294264 30-31 6.928470470858853E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012471246847545935 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01177839980046005 92-93 0.004157082282515312 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.018706870271318905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 72166.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.04373250561206 #Duplication Level Percentage of deduplicated Percentage of total 1 72.57000976880495 24.7055400049885 2 10.37935525887333 7.06703988027603 3 4.32269619016607 4.414821384031261 4 2.641647671768154 3.5972618684699165 5 1.6769781830022794 2.8545298339938476 6 1.0582872028655161 2.1616827869079622 7 1.0094431781178768 2.4055649474821936 8 0.7530120481927711 2.0508272593742207 9 0.6349723217193096 1.9455145082171659 >10 4.5791273200911755 29.99612005653632 >50 0.24422012373819602 5.710445362081867 >100 0.10989905568218822 6.694288168943824 >500 0.0081406707912732 1.915029238145387 >1k 0.012211006186909802 4.481334700551506 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1139 1.5783055732616469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 1.456364492974531 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1044 1.4466646343153284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 1.0392705706288279 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 632 0.875758667516559 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.6055483191530638 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 367 0.5085497325610399 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.4143225341573594 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 253 0.35058060582545797 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 247 0.3422664412604273 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 202 0.27991020702269764 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.26605326608097996 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 190 0.2632818778926364 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 183 0.253582019233434 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 182 0.25219632513926227 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 163 0.22586813734999858 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 162 0.22448244325582686 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 161 0.2230967491616551 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 161 0.2230967491616551 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.2217110550674833 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 154 0.21339689050245267 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 152 0.21062550231410915 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 137 0.1898400909015326 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 133 0.1842973145248455 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 126 0.1745974558656431 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 125 0.17321176177147135 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 117 0.16212620901809716 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 111 0.15381204445306654 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.15381204445306654 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 104 0.14411218579386414 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 101 0.13995510351134882 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 101 0.13995510351134882 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 96 0.13302663304048998 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.1316409389463182 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 92 0.1274838566638029 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 90 0.12471246847545936 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 88 0.12194108028711582 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 87 0.12055538619294404 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 87 0.12055538619294404 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 87 0.12055538619294404 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 86 0.11916969209877229 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 85 0.1177839980046005 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 84 0.11639830391042873 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 84 0.11639830391042873 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 82 0.11362691572208519 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 81 0.11224122162791343 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 81 0.11224122162791343 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 80 0.11085552753374164 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 79 0.10946983343956987 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 77 0.10669844525122633 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 76 0.10531275115705457 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 75 0.1039270570628828 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 74 0.10254136296871104 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 73 0.10115566887453925 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0027713881883435413 0.0 24-25 0.0 0.0 0.0 0.006235623423772968 0.0 26-27 0.0 0.0 0.0 0.008314164565030624 0.0 28-29 0.0 0.0 0.0 0.016628329130061247 0.0 30-31 0.0 0.0 0.0 0.04503505806058254 0.0 32-33 0.0 0.0 0.0 0.06859185766150265 0.0 34-35 0.0 0.0 0.0 0.10184851592162514 0.0 36-37 0.0 0.0 0.0 0.14341933874677826 0.0 38-39 0.0 0.0 0.0 0.20369703184325028 0.0 40-41 0.0 0.0 0.0 0.25912479561012114 0.0 42-43 0.0 0.0 0.0 0.31732394756533544 0.0 44-45 0.0 0.0 0.0 0.3540448410608874 0.0 46-47 0.0 0.0 0.0 0.3970013579802123 0.0 48-49 0.0 0.0 0.0 0.44134356899370897 0.0 50-51 0.0 0.0 0.0 0.48014300363051854 0.0 52-53 0.0 0.0 0.0 0.550120555386193 0.0 54-55 0.0 0.0 0.0 0.640190671507358 0.0 56-57 0.0 0.0 0.0 0.7988526452900258 0.0 58-59 0.0 0.0 0.0 0.9630573954493806 0.0 60-61 0.0 0.0 0.0 1.1771471329989192 0.0 62-63 0.0 0.0 0.0 1.3067095308039796 0.0 64-65 0.0 0.0 0.0 1.4889283041875676 0.0 66-67 0.0 0.0 0.0 1.763988581880664 0.0 68-69 0.0 0.0 0.0 2.138818834354128 0.0 70-71 0.0 0.0 0.0 2.4907851342737577 0.0 72-73 0.0 0.0 0.0 2.857994069229277 0.0 74-75 0.0 0.0 0.0 3.256381121303661 0.0 76-77 0.0 0.0 0.0 3.6277471385416957 0.0 78-79 0.0 0.0 0.0 4.002577391015159 0.0 80-81 0.0 0.0 0.0 4.377407643488623 0.0 82-83 0.0 0.0 0.0 4.78549455422221 0.0 84-85 0.0 0.0 0.0 5.37649308538647 0.0 86-87 0.0 0.0 0.0 6.036083474212234 0.0 88-89 0.0 0.0 0.0 6.736551838816062 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTAGG 60 8.213691E-5 39.583336 1 ACACTGA 85 3.5778976E-7 39.117645 6 CACTGAC 90 5.5999226E-7 36.944443 7 GGGTAGT 55 0.0025636998 34.545456 4 CATGGGC 70 2.034386E-4 33.928574 4 GTACCTG 30 0.003939612 31.666668 90-91 GGTAGTA 60 0.0039266692 31.666668 5 AACACTG 135 1.164517E-8 31.666666 5 TTCCAAC 95 3.4461777E-5 30.0 1 TGGGTAG 65 0.0058083152 29.23077 3 AACGACT 65 0.0058083152 29.23077 7 CGACTCG 65 0.0058083152 29.23077 9 TAGGACA 65 0.0058083152 29.23077 4 ACGACTC 65 0.0058083152 29.23077 8 CTATAAC 100 4.889931E-5 28.5 3 ATAACTT 100 4.889931E-5 28.5 5 CAGAACG 70 0.0083404565 27.142859 4 GAACGAC 70 0.0083404565 27.142859 6 ACATGGA 70 0.0083404565 27.142859 3 GCAGAAC 70 0.0083404565 27.142859 3 >>END_MODULE