FastQCFastQC Report
Thu 26 May 2016
SRR937663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596829
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83280.5215336144321027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.3934046788979909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41440.2595143249527658No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21120.13226212700295398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.048.886911
GTATCAA75600.042.339331
ATCAACG92050.034.4510653
TCAACGC92900.034.03674
CAACGCA94150.033.584815
TATCAAC95750.033.3194662
AACGCAG96700.032.8607646
ACGCAGA109000.028.9356487
CGCAGAG109300.028.8127678
GCAGAGT119150.025.872739
GTACATG88800.025.3337141
GAGTACT64650.024.39124312-13
TACATGG91400.024.2932132
ATCACGC1000.001644927123.7722683
ACTGCGT1801.952103E-723.7498918
ACATGGG91900.023.0738453
GTACTTT69550.022.56966214-15
GTATAGG4900.022.3245371
AGTACTT67650.022.25644512-13
CCGATAA1100.00287792621.5908099
Produced by FastQC (version 0.11.5)