FastQCFastQC Report
Thu 26 May 2016
SRR937663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596829
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105820.662688365504384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90450.5664351035708896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60190.37693453713578595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21840.13677106315078197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49350.049.8780861
GTATCAA86300.040.1956631
ATCAACG108700.031.7773443
TATCAAC110350.031.3883082
TCAACGC110100.031.373274
CAACGCA111450.030.9932445
AACGCAG114350.030.2393156
ACGCAGA128500.026.7985697
CGCAGAG129400.026.5754728
GCAGAGT138950.024.4071129
GAGTACT83200.024.17724612-13
GTACATG88250.024.1767961
TACATGG89450.023.7432372
GTGTAGC12050.022.8722081
GTATAGC12350.022.7013761
ACATGGG89800.022.6983183
GTACTTT89700.022.37231814-15
ATAGCCC9700.022.0421283
TAGCCCT17600.021.8667934
TAGGACG6100.021.8092614
Produced by FastQC (version 0.11.5)