FastQCFastQC Report
Thu 26 May 2016
SRR937662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648788
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.5233541243628653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65790.3990203713273023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42200.25594557941954943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22680.1375555862851986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.046.031981
GTATCAA76650.039.213771
ATCAACG94750.031.6571983
TCAACGC97350.030.7675514
TATCAAC97950.030.625762
CAACGCA98250.030.388015
AACGCAG100950.029.7410146
GTACATG93550.026.7917181
ACGCAGA114000.026.086427
CGCAGAG113850.025.9956118
TACATGG95350.025.8268262
GTATAGC11050.025.393551
ACATGGG96350.024.0763383
GCAGAGT124750.023.2672829
TAGCCCT17900.022.8419824
GAGTACT66000.022.81501612-13
AGTACTT69200.021.0735512-13
ATAGCCC9500.021.0159023
GTGTAGC11850.020.869821
GTACTTT72950.020.6739714-15
Produced by FastQC (version 0.11.5)