##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648788 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.615610375621365 33.0 31.0 34.0 30.0 34.0 2 31.72301108450571 33.0 31.0 34.0 30.0 34.0 3 31.772888327668568 33.0 31.0 34.0 30.0 34.0 4 35.36916935348874 37.0 35.0 37.0 33.0 37.0 5 35.36529195991237 37.0 35.0 37.0 33.0 37.0 6 35.45764889118553 37.0 35.0 37.0 33.0 37.0 7 35.3984563206428 37.0 35.0 37.0 33.0 37.0 8 35.390609951067084 37.0 35.0 37.0 33.0 37.0 9 36.994096876008314 39.0 37.0 39.0 33.0 39.0 10-11 36.875493999228524 39.0 37.0 39.0 33.0 39.0 12-13 36.759096681926366 39.0 37.0 39.0 33.0 39.0 14-15 37.86551424440256 40.0 38.0 41.0 33.0 41.0 16-17 37.80717715073132 40.0 37.5 41.0 33.0 41.0 18-19 37.79287088455278 40.0 37.5 41.0 33.0 41.0 20-21 37.651368762994395 40.0 37.0 41.0 32.0 41.0 22-23 37.50001213012224 40.0 37.0 41.0 32.0 41.0 24-25 37.3621966559679 39.5 37.0 41.0 32.0 41.0 26-27 37.4781818523667 40.0 37.0 41.0 32.0 41.0 28-29 37.42816480954495 40.0 37.0 41.0 32.0 41.0 30-31 37.22998408527961 39.0 36.5 41.0 31.5 41.0 32-33 36.992940268852024 39.0 36.0 41.0 31.0 41.0 34-35 36.749222155910886 39.0 36.0 41.0 30.5 41.0 36-37 36.743404549281045 39.0 36.0 41.0 30.0 41.0 38-39 36.69388514472449 39.0 36.0 41.0 30.0 41.0 40-41 36.49996694541687 39.0 35.0 41.0 30.0 41.0 42-43 36.25506190001383 39.0 35.0 41.0 30.0 41.0 44-45 35.99878850404055 39.0 35.0 41.0 29.5 41.0 46-47 35.61952901161338 39.0 35.0 40.0 27.5 41.0 48-49 35.28402499290388 39.0 35.0 40.0 26.0 41.0 50-51 34.40375506129351 38.0 34.0 39.5 24.5 40.5 52-53 34.51480299468459 38.0 34.0 39.5 24.5 40.5 54-55 34.831559909460765 38.0 34.5 40.0 25.0 41.0 56-57 34.58337275623064 38.0 34.0 40.0 23.5 41.0 58-59 34.37490872083009 38.0 34.0 40.0 23.0 41.0 60-61 34.10016205843323 38.0 34.0 40.0 22.5 41.0 62-63 33.75647839503927 38.0 33.0 40.0 22.0 41.0 64-65 33.41295363624674 37.0 33.0 40.0 20.5 41.0 66-67 33.0190358008428 36.5 32.5 39.5 18.5 41.0 68-69 32.54557499205477 36.0 32.0 39.0 17.5 40.5 70-71 32.11334507529167 36.0 31.0 39.0 13.0 40.0 72-73 31.64043315453533 35.0 31.0 38.0 9.5 40.0 74-75 31.134152480488698 35.0 30.5 37.5 7.5 39.5 76-77 30.64838717894599 35.0 30.0 37.0 4.5 39.0 78-79 30.088747006892334 34.0 29.5 36.5 2.0 39.0 80-81 29.53648619470787 34.0 29.0 36.0 2.0 37.5 82-83 28.9899893133623 34.0 29.0 35.5 2.0 37.0 84-85 28.47135714233728 34.0 27.5 35.0 2.0 37.0 86-87 27.911397644815466 33.5 26.5 35.0 2.0 36.0 88-89 27.39663013073846 33.0 26.0 35.0 2.0 36.0 90-91 26.85290194979585 33.0 25.5 35.0 2.0 35.0 92-93 26.269909169644613 32.0 24.5 35.0 2.0 35.0 94-95 25.612107196316323 32.0 21.5 34.0 2.0 35.0 96-97 24.800510738797225 32.0 18.0 34.0 2.0 35.0 98-99 23.814768787739844 31.0 2.0 34.0 2.0 35.0 100-101 21.26333979868849 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.54173465158064 1101 2 0.6385897471494495 1101 3 0.6025144369128803 1101 4 0.4641265556025118 1101 5 0.5812874422236902 1101 6 0.4392710005585627 1101 7 0.417778210559554 1101 8 0.5221625200537687 1101 9 0.7321157713219435 1101 10-11 0.5042300608200208 1101 12-13 0.6589031402443908 1101 14-15 0.8740016086447824 1101 16-17 0.9589620656712299 1101 18-19 0.9138622183373073 1101 20-21 1.0540807728848094 1101 22-23 0.972033084524945 1101 24-25 0.8951254481390691 1101 26-27 0.9538104936561567 1101 28-29 0.8717435052716311 1101 30-31 0.8738966669000305 1101 32-33 0.911612762904987 1101 34-35 0.9905725990890204 1101 36-37 0.9765575807194296 1101 38-39 1.1830685473571876 1101 40-41 1.0799658592963013 1101 42-43 1.0562149479623741 1101 44-45 1.0959866163126577 1101 46-47 1.1200807479916222 1101 48-49 1.3663382442091816 1101 50-51 1.3281272688288865 1101 52-53 1.3487538459967539 1101 54-55 1.4290093165407143 1101 56-57 1.6570563269687 1101 58-59 1.6701741183109107 1101 60-61 1.6531277702452272 1101 62-63 1.448611519981064 1101 64-65 1.6541710220982893 1101 66-67 1.769530252572146 1101 68-69 1.8972171279977488 1101 70-71 1.7983651505476814 1101 72-73 1.724867111336554 1101 74-75 1.7446719782349547 1101 76-77 1.6475707026807598 1101 78-79 1.9518080709427608 1101 80-81 2.098663472410312 1101 82-83 2.1505847835983083 1101 84-85 2.1724845451361325 1101 86-87 2.2855869198497523 1101 88-89 2.321139418915571 1101 90-91 2.273712235232864 1101 92-93 2.5245089761046664 1101 94-95 2.6370835470186798 1101 96-97 2.7667248767395733 1101 98-99 3.010486036101497 1101 100-101 3.129484445567577 1102 1 0.4245896774237039 1102 2 0.43652591659319384 1102 3 0.4560637917399255 1102 4 0.3755661768118941 1102 5 0.38113382961660847 1102 6 0.36728271126084167 1102 7 0.45193261833777143 1102 8 0.39068458605296996 1102 9 0.4231520238972166 1102 10-11 0.5411187730042073 1102 12-13 0.5397675274204516 1102 14-15 0.7580954749837474 1102 16-17 0.6309358973118151 1102 18-19 0.5470786856087884 1102 20-21 0.6342728790040155 1102 22-23 0.689264804624699 1102 24-25 0.5903517104732572 1102 26-27 0.6073968962296235 1102 28-29 0.5971636809321694 1102 30-31 0.585381468287963 1102 32-33 0.6496282687422621 1102 34-35 0.7605494668376025 1102 36-37 0.7605872912048426 1102 38-39 0.9355923121311065 1102 40-41 0.7963445776249927 1102 42-43 0.9443370790210395 1102 44-45 0.9730377964189216 1102 46-47 0.9280749287846248 1102 48-49 0.9948270575969715 1102 50-51 0.989124124288459 1102 52-53 1.0519056152525508 1102 54-55 1.0511810373633637 1102 56-57 1.2779575811704618 1102 58-59 1.3040592522249526 1102 60-61 1.3390423977798278 1102 62-63 1.392196838230241 1102 64-65 1.4720443007334865 1102 66-67 1.203824718903867 1102 68-69 1.103117659316645 1102 70-71 1.272617870394427 1102 72-73 1.2149337972800929 1102 74-75 1.2406256414438275 1102 76-77 1.3953803676245542 1102 78-79 1.3980011890979611 1102 80-81 1.5077130621742896 1102 82-83 1.506140138353068 1102 84-85 1.7937960353591436 1102 86-87 1.8113604045065657 1102 88-89 1.7038372177372736 1102 90-91 1.9419587021429017 1102 92-93 2.2719779825021824 1102 94-95 2.195019897183137 1102 96-97 2.598564252168785 1102 98-99 2.5411913890490503 1102 100-101 2.8177610928368857 1103 1 0.13446384020024027 1103 2 0.34096066390393176 1103 3 0.23294120192868562 1103 4 0.19899659423967364 1103 5 0.24690914928375207 1103 6 0.3157198941307442 1103 7 0.403833707573952 1103 8 0.3711087792740031 1103 9 0.3788316125583364 1103 10-11 0.48202686447298504 1103 12-13 0.4100139586497349 1103 14-15 0.6043888583813484 1103 16-17 0.5233763614211426 1103 18-19 0.5632168021097002 1103 20-21 0.5397850229726231 1103 22-23 0.5155350163830406 1103 24-25 0.49386974037651044 1103 26-27 0.3008995347520411 1103 28-29 0.39062741816240276 1103 30-31 0.44304840557231984 1103 32-33 0.33149772953652956 1103 34-35 0.46287677892744483 1103 36-37 0.4647776369189387 1103 38-39 0.3940186702936117 1103 40-41 0.6558703201322587 1103 42-43 0.716761136933215 1103 44-45 0.6439142594457792 1103 46-47 0.6040770598988985 1103 48-49 0.6943018901522819 1103 50-51 0.6324212625064476 1103 52-53 0.7460272917291064 1103 54-55 0.6171415610085802 1103 56-57 0.8502640895257798 1103 58-59 0.7891414523467333 1103 60-61 0.7987854801152636 1103 62-63 0.8386931848914898 1103 64-65 1.0725500175320803 1103 66-67 1.2350641479005589 1103 68-69 1.4020994602773413 1103 70-71 1.346999678120568 1103 72-73 1.2189617723832704 1103 74-75 1.2833003589866223 1103 76-77 1.418311833695867 1103 78-79 1.4510397182908434 1103 80-81 1.5586213227791212 1103 82-83 1.658439542994163 1103 84-85 1.6488675799095809 1103 86-87 1.6707203936818544 1103 88-89 1.8391452144898608 1103 90-91 1.8118093550080658 1103 92-93 2.052099422188263 1103 94-95 2.0159428726245814 1103 96-97 2.202288628056749 1103 98-99 2.392181680639606 1103 100-101 2.3924278948123963 1104 1 0.004739489518581053 1104 2 0.20183736805359942 1104 3 0.13532203584250624 1104 4 0.10058298757897433 1104 5 0.21225644752237827 1104 6 0.22810918659765633 1104 7 0.07919609852496023 1104 8 0.22732830360477863 1104 9 0.2941946669252715 1104 10-11 0.24299603221771093 1104 12-13 0.22909089831205165 1104 14-15 0.4776287329957398 1104 16-17 0.49263491119392455 1104 18-19 0.4122478002189709 1104 20-21 0.502774706854936 1104 22-23 0.45426606756557675 1104 24-25 0.424346238867237 1104 26-27 0.5357151880508795 1104 28-29 0.4652264661605372 1104 30-31 0.43354994801434543 1104 32-33 0.4521610625381527 1104 34-35 0.5736730438957736 1104 36-37 0.4238253862803987 1104 38-39 0.6117559244150854 1104 40-41 0.8059706617914699 1104 42-43 0.6103732708105554 1104 44-45 0.6283214973135571 1104 46-47 0.6318397726066252 1104 48-49 0.6088518453717029 1104 50-51 0.49253918468775737 1104 52-53 0.5400553044666907 1104 54-55 0.37633028625486986 1104 56-57 0.807255249508529 1104 58-59 0.8676816055958128 1104 60-61 0.9168822227430553 1104 62-63 0.8444935132823801 1104 64-65 0.7830178916157351 1104 66-67 0.908443754494904 1104 68-69 0.9912210975878537 1104 70-71 1.082083185600581 1104 72-73 0.9699689206898974 1104 74-75 0.9455412121395028 1104 76-77 0.981617469414175 1104 78-79 0.8511089623283965 1104 80-81 0.9858752783965983 1104 82-83 0.8706675424634298 1104 84-85 1.1723587233069317 1104 86-87 1.2557777791596827 1104 88-89 1.3517322878304938 1104 90-91 1.5699872002331325 1104 92-93 1.6521773735598764 1104 94-95 1.7570302942585734 1104 96-97 1.8232981812461198 1104 98-99 1.9140648696799758 1104 100-101 1.6439313611606217 1105 1 0.22174808329843643 1105 2 0.1706106179724891 1105 3 0.21648686745109558 1105 4 0.26160638650062396 1105 5 0.3124907863770119 1105 6 0.21492213751903222 1105 7 0.2456933668754715 1105 8 0.2022043650207479 1105 9 0.2981236237108078 1105 10-11 0.3990732713449816 1105 12-13 0.34082650196227604 1105 14-15 0.32126080635794807 1105 16-17 0.42946932826992423 1105 18-19 0.2921312487567107 1105 20-21 0.4897157229033198 1105 22-23 0.3664991737425467 1105 24-25 0.3438959293561865 1105 26-27 0.29781185404808497 1105 28-29 0.33746076761202914 1105 30-31 0.3678586361965017 1105 32-33 0.44616456542429717 1105 34-35 0.4461073708789698 1105 36-37 0.2812756868472164 1105 38-39 0.2664360339900398 1105 40-41 0.4490503069169023 1105 42-43 0.2394218566869597 1105 44-45 0.41092057703349383 1105 46-47 0.4152220823811277 1105 48-49 0.5593813031968082 1105 50-51 0.510462407408049 1105 52-53 0.29543078735353134 1105 54-55 0.39637390157813 1105 56-57 0.5197774982833749 1105 58-59 0.6487483759071466 1105 60-61 0.4748695024202121 1105 62-63 0.6848597226277988 1105 64-65 0.6515935959942638 1105 66-67 0.6418213075646904 1105 68-69 0.8405593871093586 1105 70-71 0.9364038183967978 1105 72-73 0.6905533774051058 1105 74-75 0.6994557361885114 1105 76-77 0.6468300209349778 1105 78-79 0.7488883943371931 1105 80-81 0.5970260513001264 1105 82-83 0.6294263840391423 1105 84-85 0.6897072791795118 1105 86-87 0.6818337879347851 1105 88-89 0.6020863980705791 1105 90-91 0.7006514394896861 1105 92-93 1.0364158495279447 1105 94-95 1.027936418048359 1105 96-97 0.9410197115552705 1105 98-99 1.3225590839821244 1105 100-101 1.4979295950815406 1106 1 0.3362534387764704 1106 2 0.29549892769669484 1106 3 0.21560384776613617 1106 4 0.23192949253246553 1106 5 0.2542405710753286 1106 6 0.15570407447481927 1106 7 0.15780180460117776 1106 8 0.26060848265544934 1106 9 0.36435333162761196 1106 10-11 0.027281791636660557 1106 12-13 -0.016942899830262093 1106 14-15 0.17756712612901993 1106 16-17 0.14437777859865264 1106 18-19 0.09789398225603207 1106 20-21 0.03547581461457128 1106 22-23 -0.04000515895148027 1106 24-25 0.030780622981481542 1106 26-27 -0.03737167347417625 1106 28-29 0.33679527130325937 1106 30-31 0.3467511937866732 1106 32-33 0.4242346947992033 1106 34-35 0.25516304878441076 1106 36-37 0.4051812029330364 1106 38-39 0.41511154485692003 1106 40-41 0.31498804473842057 1106 42-43 0.26840901775847925 1106 44-45 0.33899203942073086 1106 46-47 0.5255646019198537 1106 48-49 0.45993615948192 1106 50-51 0.3843937758542779 1106 52-53 0.36461291561678877 1106 54-55 0.3435381400079862 1106 56-57 0.3886424100056374 1106 58-59 0.40783830819012223 1106 60-61 0.4232029542435285 1106 62-63 0.6513019450259634 1106 64-65 0.680819083513228 1106 66-67 0.8963834412892595 1106 68-69 0.8540884524334231 1106 70-71 0.5557655801705295 1106 72-73 0.6135278853619788 1106 74-75 0.6175960547219077 1106 76-77 0.7841529120690751 1106 78-79 0.8344875793415341 1106 80-81 0.695149897032632 1106 82-83 0.6742501355721053 1106 84-85 0.7655251832317056 1106 86-87 0.6562140987771059 1106 88-89 1.1138807296174065 1106 90-91 1.293978460538355 1106 92-93 1.2695471843661004 1106 94-95 1.2547825997355169 1106 96-97 1.3456196543569234 1106 98-99 1.5720074996363778 1106 100-101 1.4812223052006352 1107 1 0.4434545804619958 1107 2 0.4524422220230129 1107 3 0.5540045199153347 1107 4 0.45047306553793476 1107 5 0.45534249468750687 1107 6 0.5336289521205018 1107 7 0.4696925181200555 1107 8 0.35622853510660235 1107 9 0.6295782543944171 1107 10-11 0.5717068689562907 1107 12-13 0.5633132792573932 1107 14-15 0.7073096850328398 1107 16-17 0.6964276348506928 1107 18-19 0.8090828980178273 1107 20-21 0.7950011697489572 1107 22-23 0.7280102289086869 1107 24-25 0.7101833471911192 1107 26-27 0.7440653047587702 1107 28-29 0.8254482155000105 1107 30-31 0.7127680269059269 1107 32-33 0.8454194528479633 1107 34-35 1.0069609787446652 1107 36-37 0.9657965098863599 1107 38-39 1.0021890529259991 1107 40-41 1.09078358552636 1107 42-43 1.1925646821886815 1107 44-45 1.1434930879250729 1107 46-47 1.192329736946121 1107 48-49 1.2382436158777992 1107 50-51 1.1369696562781044 1107 52-53 1.0736002315666724 1107 54-55 1.0457620410665172 1107 56-57 1.0539151767259582 1107 58-59 1.0330114021682633 1107 60-61 1.0981015613664482 1107 62-63 1.1161988752334295 1107 64-65 1.0210312997557978 1107 66-67 1.176546309540754 1107 68-69 1.1410076043636934 1107 70-71 1.0689736663605274 1107 72-73 0.962316789368078 1107 74-75 1.0243022902541838 1107 76-77 1.015849898634734 1107 78-79 1.0436045537020178 1107 80-81 1.1800079556486054 1107 82-83 1.4180047060310805 1107 84-85 1.4649240436207158 1107 86-87 1.4395937921954705 1107 88-89 1.4736707175678205 1107 90-91 1.4327112310965582 1107 92-93 1.4977040641278059 1107 94-95 1.5307927251929954 1107 96-97 1.776882544563911 1107 98-99 1.966669765408458 1107 100-101 1.9674916956159443 1108 1 0.08757198666632249 1108 2 0.07754406819951143 1108 3 0.0924856242959855 1108 4 0.2543353207038592 1108 5 0.23908201332008616 1108 6 0.134444465991848 1108 7 0.18342969584153224 1108 8 0.2785408920566681 1108 9 0.21444993027996873 1108 10-11 0.39538787308307377 1108 12-13 0.26375958264443256 1108 14-15 0.2650414745228957 1108 16-17 0.31175865870565644 1108 18-19 0.23261906344000494 1108 20-21 0.18208798446377727 1108 22-23 0.2155279201582303 1108 24-25 0.27606531233222853 1108 26-27 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2316 54-55 -1.0297449393055729 2316 56-57 -0.8189842574209294 2316 58-59 -0.6992796947675259 2316 60-61 -0.9485034376958268 2316 62-63 -0.7795334105667209 2316 64-65 -0.6536557389828985 2316 66-67 -0.816274002474934 2316 68-69 -0.936350110377596 2316 70-71 -0.7774636405522024 2316 72-73 -0.665301683919278 2316 74-75 -0.8121301828558245 2316 76-77 -0.7625957748371803 2316 78-79 -0.7050732129241872 2316 80-81 -0.6755564889074428 2316 82-83 -0.7237946221017317 2316 84-85 -0.8581279770422192 2316 86-87 -0.6656191030718759 2316 88-89 -0.7676551176167692 2316 90-91 -0.8218385072424788 2316 92-93 -0.6313461093319468 2316 94-95 -0.4825592815806097 2316 96-97 -0.7387198092469056 2316 98-99 -0.5959428892673415 2316 100-101 -0.23538304376283392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5907.0 3 1049.0 4 998.0 5 1091.0 6 1331.0 7 1708.0 8 2001.0 9 2610.0 10 3310.0 11 4385.0 12 6044.0 13 7637.0 14 9313.0 15 11430.0 16 15170.0 17 19807.0 18 16830.0 19 11651.0 20 11182.0 21 11707.0 22 12909.0 23 14545.0 24 16523.0 25 19145.0 26 22208.0 27 25817.0 28 30427.0 29 36634.0 30 44378.0 31 54542.0 32 68508.0 33 87706.0 34 116039.0 35 159080.0 36 222271.0 37 303139.0 38 242705.0 39 27047.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84741621067759 19.839160034974203 15.029963547241035 22.28346020710716 2 14.4433490226655 20.780407938854786 42.23657826187176 22.539664776607953 3 18.663542484478924 25.856601010595504 30.012156579665128 25.46769992526045 4 11.715882980444437 17.665629914890665 38.99995012887429 31.61853697579061 5 12.768382473686323 38.60675269826944 34.575982756376646 14.048882071667594 6 29.87229263050925 37.588584376086416 18.70938489926604 13.829738094138296 7 27.45021893640361 33.748172997742266 21.18356320896044 17.618044856893686 8 25.131892006867318 36.06976708853067 20.38037648703262 18.417964417569394 9 26.388205970071237 17.020896404136128 21.23172149635296 35.359176129439675 10-11 24.004790668104313 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30.725596261331223 20.317053893023484 36-37 20.567382997651933 28.194229731503494 30.44181846466473 20.796568806179845 38-39 20.444901640833415 28.412007805419865 30.545768737549594 20.597321816197127 40-41 21.037355327511143 28.004510795316286 30.501002685879676 20.457131191292902 42-43 20.609432472883967 28.12620585763481 30.437533004418416 20.82682866506281 44-45 20.87993590212982 28.38630816503439 29.978819385048144 20.754936547787644 46-47 20.837645334440122 28.384097967854906 29.567829625771978 21.210427071932987 48-49 20.827220071353466 28.476263120468236 29.538694365264114 21.15782244291419 50-51 21.052605989591722 28.817488047132013 29.01467162507346 21.115234338202807 52-53 21.023610248685543 28.890603102178964 29.043392999505492 21.04239364963 54-55 21.08919892218931 28.680089064013544 29.05087514387217 21.179836869924973 56-57 21.243346387644067 28.891586638000042 28.625305433503673 21.239761540852214 58-59 21.150038689261883 28.866199560033067 28.819717761302897 21.164043989402153 60-61 21.253319497410928 28.783224592136975 28.982379248060553 20.981076662391544 62-63 21.224239574162745 28.690608772048144 29.06584716933787 21.01930448445124 64-65 21.235616828701545 28.712481176603184 28.922094149033605 21.129807845661663 66-67 21.133649187742193 28.87494790103445 28.78946306775721 21.201939843466153 68-69 21.369742162857268 28.744832882658322 28.860486388764084 21.024938565720326 70-71 21.234310386102123 28.578398550522106 28.988099650390016 21.19919141298576 72-73 21.149960583455986 28.7190002931558 28.834591776751985 21.296447346636228 74-75 21.0663295363638 28.87498560603091 28.674092891308593 21.384591966296693 76-77 20.95645511351346 28.73685438535517 28.96574058996376 21.340949911167606 78-79 20.899326465216905 28.733524313514963 28.81561601742216 21.551533203845974 80-81 20.84020047131478 28.990689452045682 28.651892079426595 21.517217997212942 82-83 21.072616548574803 28.92554578063192 28.73925759922435 21.262580071568923 84-85 21.13713963398206 28.729774807023663 28.66814509135565 21.464940467638627 86-87 20.801542601049423 28.867422698324656 28.598232983060672 21.732801717565252 88-89 21.17842327684879 28.507238170688144 28.683484042488562 21.630854509974508 90-91 21.05809661209676 28.62916614642525 28.811015204416147 21.501722037061846 92-93 20.965618430805495 28.598946498657302 28.968097257494197 21.467337813043002 94-95 20.836150839723224 28.6902043125011 28.83419331830258 21.639451529473092 96-97 20.990852343357925 28.54772444750122 28.804546608938892 21.656876600201958 98-99 21.033660893724907 28.343417372903883 28.880831109173467 21.742090624197747 100-101 21.264238102600913 27.966721335229266 29.205284755976606 21.563755806193214 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2597.0 1 1511.5 2 471.5 3 513.0 4 469.5 5 476.5 6 776.0 7 1511.5 8 2315.0 9 2645.5 10 2319.5 11 1925.5 12 1926.0 13 2058.5 14 2168.5 15 2176.0 16 2257.0 17 2419.0 18 2598.0 19 2836.5 20 3121.5 21 3583.5 22 4231.5 23 5192.5 24 6384.0 25 7750.5 26 9736.0 27 12299.0 28 15058.5 29 18644.5 30 23059.5 31 27809.5 32 33061.0 33 38944.0 34 45294.5 35 50926.5 36 56872.0 37 61958.5 38 63971.0 39 64548.5 40 64563.0 41 64665.5 42 64391.5 43 64452.0 44 64630.0 45 64246.5 46 63324.0 47 61962.0 48 61488.0 49 60045.0 50 56683.5 51 51930.5 52 46430.0 53 42085.0 54 38112.0 55 33827.5 56 29884.0 57 25734.0 58 21944.5 59 18456.0 60 15057.0 61 12333.5 62 9673.5 63 6851.0 64 4789.5 65 3564.5 66 2667.5 67 2006.0 68 1484.5 69 1071.0 70 775.5 71 592.5 72 481.5 73 422.0 74 332.5 75 215.0 76 154.0 77 102.0 78 62.0 79 45.0 80 34.0 81 24.0 82 17.0 83 13.5 84 8.0 85 3.5 86 4.0 87 4.5 88 4.5 89 2.5 90 1.5 91 4.5 92 4.5 93 3.0 94 2.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3209023840542265 2 0.2772339439636872 3 0.2675298461657896 4 0.2759602811277132 5 0.2781437031322402 6 0.21009371732448318 7 0.20214848725245452 8 0.20093547502771733 9 0.21112477771550983 10-11 0.21264104299643133 12-13 0.209517536517733 14-15 0.20972981365706203 16-17 0.1953556187939262 18-19 0.2005109207490593 20-21 0.1898060878657535 22-23 0.19923725791308522 24-25 0.19820619752205865 26-27 0.19681123346361085 28-29 0.21039697038066751 30-31 0.20657598187274528 32-33 0.21303527196947092 34-35 0.20557524678733713 36-37 0.19286894373321495 38-39 0.18246736390609344 40-41 0.17943483334425045 42-43 0.19256569067703064 44-45 0.19283861842759653 46-47 0.19329349801187296 48-49 0.20972981365706203 50-51 0.20421060803450775 52-53 0.2255292978842641 54-55 0.19590147429505794 56-57 0.18022329129032963 58-59 0.18089044801393508 60-61 0.1717018804115508 62-63 0.17594742319813098 64-65 0.17573514605880197 66-67 0.17479506158463065 68-69 0.17731206195096033 70-71 0.17916190559368458 72-73 0.17622035094869687 74-75 0.19004869031070096 76-77 0.18146662882068526 78-79 0.1860457499690682 80-81 0.17992003823414532 82-83 0.16269526464287706 84-85 0.1617248548630873 86-87 0.16600072295528595 88-89 0.16933650657331326 90-91 0.1769481582835392 92-93 0.1727329408025774 94-95 0.17158057918907707 96-97 0.17600807380936784 98-99 0.18410493040948867 100-101 0.17033724165872144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1648788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.29583517888969 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71270050272442 54.44045455198264 2 12.950849616015889 17.68978181604016 3 3.7746873899663727 7.733862835109301 4 1.5033275031519486 4.10684029500629 5 0.6798049792748528 2.3213924409171933 6 0.39144212810877393 1.6040320238034387 7 0.2402282638143071 1.1484612937540923 8 0.1515461979282099 0.8279979324553932 9 0.1102407001566714 0.6776082619114885 >10 0.44783207182096296 5.302464708494762 >50 0.02559703806312625 1.1850662457491632 >100 0.010854090426709909 1.3075001202381746 >500 3.5580741916333667E-4 0.154306046826744 >1k 3.558074191633366E-4 0.5763391599391025 >5k 1.779037095816683E-4 0.9238922677720954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8629 0.5233541243628653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6579 0.3990203713273023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4220 0.25594557941954943 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2268 0.1375555862851986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.0650611236860046E-5 0.0 3 0.0 0.0 0.0 6.0650611236860046E-5 0.0 4 0.0 0.0 0.0 2.4260244494744019E-4 0.0 5 0.0 0.0 0.0 2.4260244494744019E-4 0.0 6 0.0 0.0 0.0 2.4260244494744019E-4 0.0 7 0.0 0.0 0.0 2.4260244494744019E-4 0.0 8 0.0 0.0 0.0 2.4260244494744019E-4 0.0 9 0.0 0.0 0.0 4.245542786580203E-4 0.0 10-11 0.0 0.0 0.0 4.245542786580203E-4 0.0 12-13 0.0 0.0 0.0 4.245542786580203E-4 0.0 14-15 0.0 0.0 0.0 4.245542786580203E-4 0.0 16-17 0.0 0.0 0.0 4.5487958427645035E-4 0.0 18-19 0.0 0.0 0.0 5.155301955133103E-4 0.0 20-21 0.0 0.0 0.0 6.065061123686005E-4 0.0 22-23 0.0 0.0 0.0 8.491085573160407E-4 0.0 24-25 0.0 0.0 0.0 9.704097797897607E-4 0.0 26-27 0.0 0.0 0.0 0.001303988141592491 0.0 28-29 0.0 0.0 0.0 0.0022137473101453914 0.0 30-31 0.0 0.0 0.0 0.004761072982093513 0.0 32-33 0.0 0.0 0.0 0.008005880683265526 0.0 34-35 0.0 0.0 0.0 0.011038411245108529 0.0 36-37 0.0 0.0 0.0 0.01397996589009624 0.0 38-39 0.0 0.0 0.0 0.016345339728333783 0.0 40-41 0.0 0.0 0.0 0.019044291928374056 0.0 42-43 0.0 0.0 0.0 0.022228449018309206 0.0 44-45 0.0 0.0 0.0 0.02620106405432354 0.0 46-47 0.0 0.0 0.0 0.030749859897088044 0.0 48-49 0.0 0.0 0.0 0.036875571632010905 0.0 50-51 0.0 0.0 0.0 0.04560925965011875 0.0 52-53 0.0 0.0 0.0 0.06629111808188803 0.0 54-55 0.0 0.0 0.0 0.089277699740658 0.0 56-57 0.0 0.0 0.0 0.1203914633051672 0.0 58-59 0.0 0.0 0.0 0.1643631564518907 0.0 60-61 0.0 0.0 0.0 0.22031334531789412 0.0 62-63 0.0 0.0 0.0 0.2665897616916183 0.0 64-65 0.0 0.0 0.0 0.3175666004361992 0.0 66-67 0.0 0.0 0.0 0.39468385262386674 0.0 68-69 0.0 0.0 0.0 0.49427215627479093 0.0 70-71 0.0 0.0 0.0 0.581578711150251 0.0 72-73 0.0 0.0 0.0 0.6707351096684353 0.0 74-75 0.0 0.0 0.0 0.8148712872728332 0.0 76-77 0.0 0.0 0.0 0.933898111825171 0.0 78-79 0.0 0.0 0.0 1.0373377292896357 0.0 80-81 0.0 0.0 0.0 1.1524525894171962 0.0 82-83 0.0 0.0 0.0 1.3333127121255128 0.0 84-85 0.0 0.0 0.0 1.5264545836092935 0.0 86-87 0.0 0.0 0.0 1.760535617677955 0.0 88-89 0.0 0.0 0.0 2.052325708338489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 46.03198 1 GTATCAA 7665 0.0 39.21377 1 ATCAACG 9475 0.0 31.657198 3 TCAACGC 9735 0.0 30.767551 4 TATCAAC 9795 0.0 30.62576 2 CAACGCA 9825 0.0 30.38801 5 AACGCAG 10095 0.0 29.741014 6 GTACATG 9355 0.0 26.791718 1 ACGCAGA 11400 0.0 26.08642 7 CGCAGAG 11385 0.0 25.995611 8 TACATGG 9535 0.0 25.826826 2 GTATAGC 1105 0.0 25.39355 1 ACATGGG 9635 0.0 24.076338 3 GCAGAGT 12475 0.0 23.267282 9 TAGCCCT 1790 0.0 22.841982 4 GAGTACT 6600 0.0 22.815016 12-13 AGTACTT 6920 0.0 21.07355 12-13 ATAGCCC 950 0.0 21.015902 3 GTGTAGC 1185 0.0 20.86982 1 GTACTTT 7295 0.0 20.67397 14-15 >>END_MODULE