FastQCFastQC Report
Thu 26 May 2016
SRR937662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648788
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109250.662607927762696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91260.5534974781475848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.3767615970033746No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.13779818873014604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51500.051.6686171
GTATCAA87600.040.410871
ACCGTCG600.003951731.6662528
ATCAACG113500.030.8897423
TATCAAC115000.030.5694522
TCAACGC115650.030.3565644
CAACGCA117100.029.8589635
AACGCAG120700.028.9622386
ACGCAGA135300.025.698117
CGCAGAG135400.025.644058
GAGTACT90400.024.11749512-13
TAGGACG6750.023.9292434
GCAGAGT147100.023.4106399
GTACATG87800.023.1630231
GTACTTT94950.022.81032414-15
GTGTAGC11450.021.9946161
TACATGG91550.021.7943922
AGTACTT92650.021.71180512-13
GTGGTAT23550.020.9840071
CAGAGTA144650.020.86820210-11
Produced by FastQC (version 0.11.5)