##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648788 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.153607377055145 33.0 31.0 34.0 31.0 34.0 2 32.34453307520433 34.0 31.0 34.0 31.0 34.0 3 32.44265424056944 34.0 31.0 34.0 31.0 34.0 4 36.08753945322261 37.0 35.0 37.0 35.0 37.0 5 36.007092482478036 37.0 35.0 37.0 35.0 37.0 6 36.040361768765905 37.0 35.0 37.0 35.0 37.0 7 35.98703289931756 37.0 35.0 37.0 35.0 37.0 8 35.96651661705447 37.0 35.0 37.0 35.0 37.0 9 37.63112116293908 39.0 37.0 39.0 35.0 39.0 10-11 37.56634267110144 39.0 37.0 39.0 35.0 39.0 12-13 37.47704677617741 39.0 37.0 39.0 35.0 39.0 14-15 38.67057620506699 40.0 38.0 41.0 34.0 41.0 16-17 38.63369092933719 40.0 38.0 41.0 34.0 41.0 18-19 38.655967292338374 40.0 38.0 41.0 34.0 41.0 20-21 38.575101529123216 40.0 38.0 41.0 34.0 41.0 22-23 38.416623907985745 40.0 38.0 41.0 34.0 41.0 24-25 38.500850321569544 40.0 38.0 41.0 34.0 41.0 26-27 38.390070767133196 40.0 38.0 41.0 34.0 41.0 28-29 38.254131216384394 40.0 38.0 41.0 34.0 41.0 30-31 38.25285300475258 40.0 38.0 41.0 34.0 41.0 32-33 38.14699009211615 40.0 37.0 41.0 34.0 41.0 34-35 38.02509631317064 40.0 37.0 41.0 33.5 41.0 36-37 37.85987343430447 40.0 37.0 41.0 33.0 41.0 38-39 37.719132174664054 40.0 37.0 41.0 33.0 41.0 40-41 37.529759738668645 40.0 37.0 41.0 33.0 41.0 42-43 37.302100391317744 40.0 37.0 41.0 32.5 41.0 44-45 36.95881702195795 40.0 36.0 41.0 31.5 41.0 46-47 36.56121981722332 40.0 36.0 41.0 30.5 41.0 48-49 36.20880337557042 39.0 36.0 41.0 30.0 41.0 50-51 35.964110910559754 39.0 35.5 41.0 29.0 41.0 52-53 35.73300114993559 39.0 35.0 40.0 28.5 41.0 54-55 35.526942214523636 39.0 35.0 40.0 28.0 41.0 56-57 35.282765279708485 38.5 35.0 40.0 27.0 41.0 58-59 35.052114644211386 38.0 34.5 40.0 27.0 41.0 60-61 34.734906488887596 38.0 34.0 40.0 26.0 41.0 62-63 34.41018918138657 38.0 34.0 40.0 26.0 41.0 64-65 34.04779541093215 37.5 34.0 40.0 25.0 41.0 66-67 33.62706879234929 37.0 33.0 39.5 23.5 41.0 68-69 33.20562407052938 36.0 32.5 39.0 22.5 40.0 70-71 32.81032491745452 36.0 32.0 39.0 22.0 40.0 72-73 32.31474756002591 35.0 31.5 38.0 20.5 40.0 74-75 31.804985540894283 35.0 31.0 37.5 19.5 39.0 76-77 30.52734190205169 34.0 29.5 36.0 18.0 38.5 78-79 31.128116834911463 34.5 31.0 36.0 18.0 39.0 80-81 30.904880736638063 34.0 31.0 36.0 17.5 38.5 82-83 30.516764132198922 34.0 31.0 36.0 13.5 37.0 84-85 30.080121883468344 34.0 30.0 35.0 7.5 37.0 86-87 29.633249392887382 34.0 30.0 35.0 4.5 36.0 88-89 29.226084857483194 34.0 29.0 35.0 2.0 36.0 90-91 28.669930882563435 34.0 29.0 35.0 2.0 35.5 92-93 28.181916353103006 33.5 28.5 35.0 2.0 35.0 94-95 27.68929995851498 33.0 27.0 35.0 2.0 35.0 96-97 27.11598125410908 33.0 26.5 35.0 2.0 35.0 98-99 26.213293946826397 32.5 25.0 34.0 2.0 35.0 100-101 23.53181336836513 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5211555351299069 1101 2 0.4590090793055879 1101 3 0.3338874396829965 1101 4 0.22203715555882297 1101 5 0.2608322356370465 1101 6 0.24197802052934492 1101 7 0.3329949488499224 1101 8 0.2965196433248991 1101 9 0.4887947298968882 1101 10-11 0.42766426042197025 1101 12-13 0.48964129784461363 1101 14-15 0.521488803311108 1101 16-17 0.4731848938412142 1101 18-19 0.5218345687398553 1101 20-21 0.6557573527802347 1101 22-23 0.6332632615102867 1101 24-25 0.6119490860005072 1101 26-27 0.37387472513214703 1101 28-29 0.6524663227430736 1101 30-31 0.6000320458286623 1101 32-33 0.5736457246369042 1101 34-35 0.5329694036346737 1101 36-37 0.6245879757510622 1101 38-39 0.7131372334499204 1101 40-41 0.6743800362501418 1101 42-43 0.7052212340902031 1101 44-45 0.6872756028263396 1101 46-47 0.6424781287861165 1101 48-49 0.44358588627492423 1101 50-51 0.3530678863705745 1101 52-53 0.29606283463447625 1101 54-55 0.4768910737769332 1101 56-57 0.33439283687778953 1101 58-59 0.27094398201045067 1101 60-61 0.3482436434719318 1101 62-63 0.22214242581663513 1101 64-65 0.26286414185292983 1101 66-67 0.18728856567314978 1101 68-69 0.17801843787765392 1101 70-71 0.237582216389832 1101 72-73 0.2834457428103434 1101 74-75 0.231361579966606 1101 76-77 0.33423055184187334 1101 78-79 0.3746979475215646 1101 80-81 0.4634664957317156 1101 82-83 0.3460560954081977 1101 84-85 0.324982940745155 1101 86-87 0.25870856117258256 1101 88-89 0.5106149081595355 1101 90-91 0.36501297761332907 1101 92-93 0.515337686567392 1101 94-95 0.6155988920891708 1101 96-97 0.8821750548005305 1101 98-99 1.0109104761977612 1101 100-101 1.054139468104399 1102 1 0.4367914913232056 1102 2 0.38701356117930175 1102 3 0.2669629447973918 1102 4 0.17322991227260331 1102 5 0.15666300876026185 1102 6 0.2158494196691123 1102 7 0.283276873164084 1102 8 0.3033960665005253 1102 9 0.3239756313318125 1102 10-11 0.3678149814976237 1102 12-13 0.2664412761581332 1102 14-15 0.46748132147353516 1102 16-17 0.41650545624408153 1102 18-19 0.3652653707783813 1102 20-21 0.31277293090580116 1102 22-23 0.4038437364443297 1102 24-25 0.34198035068405375 1102 26-27 0.4311622879325938 1102 28-29 0.4676730672401277 1102 30-31 0.4824253302469188 1102 32-33 0.44793187781577615 1102 34-35 0.505878464971282 1102 36-37 0.4883715446237247 1102 38-39 0.5190070422474022 1102 40-41 0.6594317223744071 1102 42-43 0.7970616938437018 1102 44-45 0.6410906171032735 1102 46-47 0.4221316871769787 1102 48-49 0.35722758938693033 1102 50-51 0.3701731019703871 1102 52-53 0.33100157031235966 1102 54-55 0.4195800172836357 1102 56-57 0.22173425445107142 1102 58-59 0.29369581632570885 1102 60-61 0.27977850449769903 1102 62-63 0.4148458269804749 1102 64-65 0.3347205050292814 1102 66-67 0.28154706858275347 1102 68-69 0.4645258293548551 1102 70-71 0.42102729536478734 1102 72-73 0.41448091106133234 1102 74-75 0.47634206212947205 1102 76-77 0.3513908872595195 1102 78-79 0.44646937196223035 1102 80-81 0.3857041796150398 1102 82-83 0.47740933211618497 1102 84-85 0.6002793227164567 1102 86-87 0.5894383114165542 1102 88-89 0.6549972047583701 1102 90-91 0.7008065222694135 1102 92-93 0.7702153386438724 1102 94-95 0.819926249590889 1102 96-97 0.7711312119552645 1102 98-99 0.7421489552522296 1102 100-101 1.6099794579840392 1103 1 0.33931500123457425 1103 2 0.38169084959010036 1103 3 0.2347831193457992 1103 4 0.21925352366560702 1103 5 0.1847784948572766 1103 6 0.15680064083141332 1103 7 0.2170922442485903 1103 8 0.20996361944011 1103 9 0.27965352863240156 1103 10-11 0.2795741655853021 1103 12-13 0.23919345801321157 1103 14-15 0.29134303583656873 1103 16-17 0.3869142580842819 1103 18-19 0.26592922978657185 1103 20-21 0.2848274265420869 1103 22-23 0.41948121022823415 1103 24-25 0.35694381729660307 1103 26-27 0.25838473566955145 1103 28-29 0.2529624590268753 1103 30-31 0.28410738829441584 1103 32-33 0.338020505107572 1103 34-35 0.38066873626551256 1103 36-37 0.44040286862074396 1103 38-39 0.3306951891928094 1103 40-41 0.3164111216031458 1103 42-43 0.44170261800309163 1103 44-45 0.3498941486640632 1103 46-47 0.39112143753217055 1103 48-49 0.27337162564970185 1103 50-51 0.281286186335457 1103 52-53 0.13251787502787948 1103 54-55 0.3604263740930591 1103 56-57 0.2518717620621942 1103 58-59 0.25289428057034513 1103 60-61 0.11910771091133654 1103 62-63 0.12635299834912672 1103 64-65 0.24250499187049712 1103 66-67 0.11944961238899054 1103 68-69 0.08386230861878374 1103 70-71 0.2050304074681577 1103 72-73 0.16321831042537838 1103 74-75 0.26901085991040574 1103 76-77 0.13692637200344748 1103 78-79 0.21151740660129548 1103 80-81 0.3294833555841912 1103 82-83 0.31369747229614475 1103 84-85 0.22622986733454908 1103 86-87 0.34358299039632456 1103 88-89 0.17611806938187513 1103 90-91 0.37241111600461707 1103 92-93 0.5009541249235561 1103 94-95 0.6520231983764866 1103 96-97 0.6723050161633672 1103 98-99 0.661937873458033 1103 100-101 0.9238663736189743 1104 1 0.1911443706577387 1104 2 0.19812283287201637 1104 3 0.10378501008119656 1104 4 0.1010187075976745 1104 5 0.03967201660073272 1104 6 0.05312034096741769 1104 7 0.039194775180355634 1104 8 0.05047594239098174 1104 9 0.16308720362494 1104 10-11 0.19474856104753968 1104 12-13 0.12144542338290165 1104 14-15 0.2007038345893477 1104 16-17 0.21923878457116786 1104 18-19 0.14190403656667883 1104 20-21 0.17198659660876814 1104 22-23 0.14956339087639492 1104 24-25 0.05220453975654493 1104 26-27 0.15071730607456857 1104 28-29 0.15064589353114144 1104 30-31 0.34815978156737515 1104 32-33 0.3061458575472855 1104 34-35 0.31857963950630364 1104 36-37 0.224715569718704 1104 38-39 0.2549973585628962 1104 40-41 0.2903948513274983 1104 42-43 0.3674808878143523 1104 44-45 0.3497523435093228 1104 46-47 0.25196704406018 1104 48-49 0.2938902510518986 1104 50-51 0.43572485353285373 1104 52-53 0.40529833411169847 1104 54-55 0.38498354396070766 1104 56-57 0.36048580326032464 1104 58-59 0.289278083270446 1104 60-61 0.3048227074284071 1104 62-63 0.4848584051889375 1104 64-65 0.3577905095007736 1104 66-67 0.29090904450495714 1104 68-69 0.15685423152984868 1104 70-71 0.2604272960474532 1104 72-73 0.14456015206799577 1104 74-75 0.03942126558924741 1104 76-77 0.11371096085142796 1104 78-79 0.3657956100709576 1104 80-81 0.3072298975286607 1104 82-83 0.5540041363091497 1104 84-85 0.5769123564711123 1104 86-87 0.6043149869465658 1104 88-89 0.7406207562163836 1104 90-91 0.6299064720931149 1104 92-93 0.5869502439403718 1104 94-95 0.3596201400290582 1104 96-97 0.5441662295511023 1104 98-99 0.6982250662135314 1104 100-101 1.1715299056453787 1105 1 0.12539858034141105 1105 2 0.17201502886776865 1105 3 0.09965765398777648 1105 4 0.06003298828228054 1105 5 -0.03200461204210825 1105 6 0.04052076524921233 1105 7 0.15890395757192977 1105 8 0.08967118926396012 1105 9 0.14620456088110956 1105 10-11 0.11881956035394836 1105 12-13 0.10873607755583237 1105 14-15 0.1627390293255857 1105 16-17 0.27779666553645654 1105 18-19 0.282429697159138 1105 20-21 0.3114283790151333 1105 22-23 0.24982517225824807 1105 24-25 0.16631504003991893 1105 26-27 0.09274628863575884 1105 28-29 0.18809416944928614 1105 30-31 0.24660738829441442 1105 32-33 0.22369996811261927 1105 34-35 0.281233612411782 1105 36-37 0.1296158293453331 1105 38-39 0.2709571192803182 1105 40-41 0.28304736876265224 1105 42-43 0.31963880553229984 1105 44-45 0.26096966962330725 1105 46-47 0.30620689418739033 1105 48-49 0.2310184048081112 1105 50-51 0.38768254244111233 1105 52-53 0.38609710273501463 1105 54-55 0.4137878303614926 1105 56-57 0.3128874512755644 1105 58-59 0.4537683881304986 1105 60-61 0.4457406945210636 1105 62-63 0.172135968260001 1105 64-65 0.20424631727460962 1105 66-67 0.3583440748299651 1105 68-69 0.14324944620359048 1105 70-71 0.12783496515643833 1105 72-73 0.10115989691347238 1105 74-75 0.0981529492269182 1105 76-77 0.11607792964337804 1105 78-79 0.22440520495420913 1105 80-81 0.2664061907628401 1105 82-83 0.35518363645672935 1105 84-85 0.2770151338174891 1105 86-87 0.3802920832100689 1105 88-89 0.5097646632028869 1105 90-91 0.2807696310491785 1105 92-93 0.4321766264948934 1105 94-95 0.4468381833077508 1105 96-97 0.736895098195312 1105 98-99 0.694370305890466 1105 100-101 0.809943304754043 1106 1 0.12529153541162685 1106 2 0.13035112247916203 1106 3 0.21338703471064235 1106 4 0.0908490018806205 1106 5 0.1569902805009349 1106 6 0.17143711832136432 1106 7 0.1520849935729558 1106 8 0.12364926969388534 1106 9 0.19202746574926977 1106 10-11 0.045569320815680214 1106 12-13 0.04982723297860758 1106 14-15 0.2449551906055376 1106 16-17 0.3358719734623037 1106 18-19 0.3032973993205488 1106 20-21 0.18871065770681383 1106 22-23 0.23268485534062933 1106 24-25 0.3681213929343272 1106 26-27 0.2963568535259924 1106 28-29 0.40758394080131666 1106 30-31 0.4140238407365686 1106 32-33 0.4202316315989094 1106 34-35 0.33797873560636305 1106 36-37 0.26601752816856816 1106 38-39 0.3089308031931992 1106 40-41 0.2057847621014588 1106 42-43 1.4059176882597058E-4 1106 44-45 0.10485756570974303 1106 46-47 0.2489744136049339 1106 48-49 0.41856475728241804 1106 50-51 0.45894174628535467 1106 52-53 0.4114324159282816 1106 54-55 0.513100884672447 1106 56-57 0.3673082856928076 1106 58-59 0.2106154261770925 1106 60-61 0.4391344065807061 1106 62-63 0.3716705449841484 1106 64-65 0.39431024531457126 1106 66-67 0.24960500755081227 1106 68-69 0.29857870832875477 1106 70-71 0.4489513751037819 1106 72-73 0.41733224490563714 1106 74-75 0.40223790493051226 1106 76-77 0.28962724867604805 1106 78-79 0.3370249538954724 1106 80-81 0.2677125428493703 1106 82-83 0.2801433229322292 1106 84-85 0.37448640942142575 1106 86-87 0.5208859038496279 1106 88-89 0.23565188389640923 1106 90-91 0.35227483626036715 1106 92-93 0.558280603072653 1106 94-95 0.8251676845672478 1106 96-97 0.6930353569451242 1106 98-99 0.8968993130467204 1106 100-101 0.9537300938747251 1107 1 0.29165235229418585 1107 2 0.31928719742518297 1107 3 0.2162326046285088 1107 4 0.13919084368698975 1107 5 0.21900719487279474 1107 6 0.2342263646256697 1107 7 0.3277103360872573 1107 8 0.21311184422585683 1107 9 0.28482010046003126 1107 10-11 0.38538413402977056 1107 12-13 0.3373389238452944 1107 14-15 0.37219331086976126 1107 16-17 0.3340744756967524 1107 18-19 0.349331321418326 1107 20-21 0.37314088678729007 1107 22-23 0.43296063877996005 1107 24-25 0.4846570757933222 1107 26-27 0.4067836803697773 1107 28-29 0.523077602233819 1107 30-31 0.4920680473394654 1107 32-33 0.44464414934049756 1107 34-35 0.4403405932137687 1107 36-37 0.323459013327863 1107 38-39 0.5690655528573885 1107 40-41 0.5830004082411904 1107 42-43 0.5755043268300142 1107 44-45 0.743519672434175 1107 46-47 0.4699384930388675 1107 48-49 0.5854424790285648 1107 50-51 0.5630682548609585 1107 52-53 0.5213620027674253 1107 54-55 0.5174526531267318 1107 56-57 0.5509141033792488 1107 58-59 0.5620115447229139 1107 60-61 0.7644830148273272 1107 62-63 0.6249457164357963 1107 64-65 0.6574772730078777 1107 66-67 0.6249289374799503 1107 68-69 0.5553159754406352 1107 70-71 0.5535500563913018 1107 72-73 0.7901170309606158 1107 74-75 0.6412357404994431 1107 76-77 0.5538710894693466 1107 78-79 0.8056921345857084 1107 80-81 0.6964263487040476 1107 82-83 0.8110329265751126 1107 84-85 0.7690449484650301 1107 86-87 0.9070468998336736 1107 88-89 0.9893875588030099 1107 90-91 1.072858368259201 1107 92-93 1.0650260818838788 1107 94-95 1.3250453734665868 1107 96-97 1.3736698360554236 1107 98-99 1.5796917403040318 1107 100-101 1.6154703171983726 1108 1 0.11381590008971898 1108 2 0.12455441284111402 1108 3 -0.00548559103361157 1108 4 0.06313894328903302 1108 5 -0.022826292729064335 1108 6 0.03393660449874858 1108 7 0.007987787819409675 1108 8 0.015335461620068713 1108 9 0.12326456089656546 1108 10-11 0.07637022008430705 1108 12-13 0.14204614038619212 1108 14-15 0.06991716773173806 1108 16-17 0.09184022080544452 1108 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-0.2902954033764047 2316 90-91 -0.18882927235070568 2316 92-93 -0.1305802974801793 2316 94-95 0.021688548584428702 2316 96-97 0.07232147189744254 2316 98-99 0.21876401114765187 2316 100-101 0.6392887623931713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 11.0 5 36.0 6 72.0 7 169.0 8 367.0 9 742.0 10 1388.0 11 2391.0 12 3577.0 13 5138.0 14 6948.0 15 10086.0 16 14092.0 17 19984.0 18 17382.0 19 9786.0 20 7651.0 21 7663.0 22 8021.0 23 9152.0 24 10740.0 25 12575.0 26 15633.0 27 19165.0 28 24210.0 29 30044.0 30 38033.0 31 47937.0 32 61163.0 33 80119.0 34 109390.0 35 155403.0 36 231375.0 37 356220.0 38 305888.0 39 26233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87195584692744 20.401929816366557 15.84206225617202 21.88405208053398 2 14.846420522226023 21.17913279330029 41.60025424736231 22.374192437111383 3 18.80077972425806 25.726048467116453 30.651605906884328 24.821565901741156 4 12.088727207445002 18.171816320391272 38.792103756465366 30.947352715698358 5 12.681629790682889 38.42580234990366 34.86745999776754 14.025107861645903 6 29.946057346365933 38.00585642302104 18.228419906015812 13.819666324597218 7 27.249470520163904 33.88846837798432 21.206546869579352 17.65551423227243 8 25.30792315324954 36.12938716196382 20.274165023035103 18.28852466175154 9 26.19275492058409 16.84358449964459 21.20970070136367 35.753959878407656 10-11 24.01428322327771 27.800489753834256 27.953450641759474 20.231776381128558 12-13 25.350961974406182 25.34529090320755 28.42638085276534 20.87736626962092 14-15 22.010834625191354 26.749345579904755 27.663896146745365 23.575923648158525 16-17 20.398711053209993 29.899598978158505 28.546969046354047 21.154720922277452 18-19 20.105313721351685 27.898614012232017 31.93069697256409 20.06537529385221 20-21 21.60508203601676 26.787252211927793 31.91777839237064 19.68988735968481 22-23 20.799067911796122 27.348825276524153 31.60026468199647 20.25184212968326 24-25 20.328878039165144 27.185643995966736 32.2686519639432 20.21682600092492 26-27 20.360079889711923 27.567136288621903 31.835856118459 20.236927703207172 28-29 20.138818971279125 27.663962017654413 31.87887183505725 20.318347176009212 30-31 20.59661399767587 27.352576559266566 32.0375026989522 20.013306744105368 32-33 20.224249569987165 27.432514064876745 31.879083302401522 20.464153062734567 34-35 20.543787315288565 27.606460017904062 31.867923589933937 19.981829076873435 36-37 20.498632935222723 27.485856277459565 31.55017503766403 20.465335749653686 38-39 20.37701667256494 27.74555711124515 31.684120412194062 20.193305803995848 40-41 20.92262297368808 27.440452092535228 31.521566736566943 20.11535819720975 42-43 20.537014693777028 27.487487268891325 31.503784301200895 20.471713736130752 44-45 20.74487441684437 27.75748003988384 31.00398595817049 20.493659585101298 46-47 20.808648534559932 27.804271986453077 30.452823528555523 20.934255950431467 48-49 20.836608466340124 27.899675397928657 30.364667865122748 20.89904827060847 50-51 21.062986872781703 28.31134142169885 29.688383224526138 20.93728848099331 52-53 21.07069589016171 28.353714471998366 29.656429345807144 20.91916029203278 54-55 21.10225814355757 28.154741543485272 29.696571057043116 21.04642925591404 56-57 21.23268728302244 28.391400228531506 29.215429758101102 21.160482730344956 58-59 21.196266590974705 28.334024750301435 29.43586440464147 21.033844254082393 60-61 21.3088038001247 28.28268400788943 29.574845280290734 20.833666911695136 62-63 21.349833938626432 28.1330589499681 29.705183443838745 20.811923667566724 64-65 21.402933548764306 28.156439760599905 29.506188788370608 20.93443790226518 66-67 21.21816146163121 28.391460879142738 29.32560159341286 21.06477606581319 68-69 21.477728778793136 28.188298427874553 29.42047699308643 20.913495800245876 70-71 21.35283614388266 28.1021271382373 29.510191728712243 21.0348449891678 72-73 21.24966945416876 28.193072729786973 29.400232170539812 21.157025645504454 74-75 21.16524380332705 28.370779020710973 29.186408440624263 21.277568735337717 76-77 20.99645315225487 28.20289812880734 29.62054551585771 21.18010320308008 78-79 20.93437725165394 28.221912095430095 29.36754149108315 21.476169161832814 80-81 20.849284444088628 28.446380007617716 29.296398324102313 21.407937224191347 82-83 21.050488006657172 28.41401475543867 29.376866567844207 21.15863067005995 84-85 21.103259170349446 28.23371969006146 29.300843018028193 21.362178121560902 86-87 20.81231789653976 28.285049381727667 29.17876646360842 21.72386625812415 88-89 21.078240501507775 28.002933063559414 29.333668124707362 21.58515831022545 90-91 20.97310373001765 28.137799596812226 29.46106465956352 21.4280320136066 92-93 20.802404496269737 28.063565943661978 29.619810710513363 21.51421884955492 94-95 20.708089821129217 28.19910746560504 29.42397688483905 21.668825828426698 96-97 20.840854009126705 27.98962025439292 29.494422569790657 21.675103166689713 98-99 20.85073902217203 27.775552208272064 29.487104450508912 21.886604319046995 100-101 20.663371697131737 27.81456772860936 29.78675713425611 21.73530344000279 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 760.0 1 522.0 2 445.5 3 580.5 4 423.5 5 365.0 6 794.5 7 1914.5 8 2932.5 9 3174.0 10 2689.5 11 2075.0 12 1960.5 13 2077.5 14 2220.5 15 2288.0 16 2449.5 17 2684.0 18 2868.5 19 3080.0 20 3406.5 21 3973.0 22 4704.0 23 5665.5 24 6829.5 25 8289.0 26 10257.0 27 12810.5 28 15687.5 29 19119.0 30 23332.5 31 27866.5 32 32962.0 33 38771.0 34 44989.0 35 50760.0 36 56786.5 37 61916.0 38 63957.0 39 64642.5 40 64874.5 41 64367.0 42 64157.5 43 64874.0 44 64905.0 45 64442.0 46 63706.0 47 62491.5 48 62077.5 49 60106.0 50 56592.5 51 51913.0 52 46357.0 53 41980.5 54 37510.5 55 33200.5 56 29145.5 57 24912.5 58 21241.0 59 17776.0 60 14277.5 61 11556.5 62 9170.5 63 6560.0 64 4630.0 65 3424.5 66 2548.5 67 1931.5 68 1422.0 69 1007.0 70 760.0 71 567.5 72 439.5 73 361.0 74 255.0 75 191.0 76 144.0 77 86.5 78 50.0 79 37.0 80 29.0 81 19.0 82 15.0 83 9.0 84 3.5 85 2.0 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 1.5 92 2.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020681858431769274 2 0.0 3 0.0 4 2.4260244494744019E-4 5 0.023047232270006816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.0325305618430023E-5 12-13 0.004215217480961772 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010310603910266207 24-25 3.0325305618430023E-5 26-27 9.097591685529007E-4 28-29 0.001334313447210921 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.097591685529007E-5 40-41 6.368314179870304E-4 42-43 0.01619371320024163 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.0325305618430023E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.0650611236860046E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002426024449474402 84-85 5.761808067501705E-4 86-87 0.0 88-89 0.0 90-91 0.010704832883305797 92-93 0.002638301588803412 94-95 0.0 96-97 0.0 98-99 0.001303988141592491 100-101 0.019135267845229346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1648788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.56854880968002 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68824803623016 52.381624801479965 2 13.37566558343975 17.807972945063323 3 4.062175319381035 8.11239348065082 4 1.6225625626838416 4.320465406031152 5 0.7773946577259965 2.5875017108608747 6 0.4179953572120014 1.669520660327205 7 0.2615067740887012 1.2185688518489974 8 0.16517199434145868 0.8796207973849269 9 0.1157826193648318 0.6936732853650368 >10 0.4733563127771848 5.474763830816657 >50 0.027081592065625402 1.2290971411994127 >100 0.01168954027412279 1.3775606851420101 >500 8.217902491714715E-4 0.3534575664242029 >1k 2.739300830571572E-4 0.2974149393832545 >5k 1.8262005537143813E-4 0.9323013162191615 >10k+ 9.131002768571907E-5 0.664062581802995 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10925 0.662607927762696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9126 0.5534974781475848 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6212 0.3767615970033746 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 0.13779818873014604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.0650611236860046E-5 0.0 10-11 0.0 0.0 0.0 6.0650611236860046E-5 0.0 12-13 0.0 0.0 0.0 9.097591685529007E-5 0.0 14-15 0.0 0.0 0.0 1.8195183371058014E-4 0.0 16-17 0.0 0.0 0.0 2.1227713932901016E-4 0.0 18-19 0.0 0.0 0.0 2.4260244494744019E-4 0.0 20-21 0.0 0.0 0.0 2.4260244494744019E-4 0.0 22-23 0.0 0.0 0.0 4.8520488989488037E-4 0.0 24-25 0.0 0.0 0.0 7.278073348423206E-4 0.0 26-27 0.0 0.0 0.0 0.001152361613500341 0.0 28-29 0.0 0.0 0.0 0.0020924460876716715 0.0 30-31 0.0 0.0 0.0 0.004791398287711943 0.0 32-33 0.0 0.0 0.0 0.008066531294502386 0.0 34-35 0.0 0.0 0.0 0.011250688384437539 0.0 36-37 0.0 0.0 0.0 0.0143438695575174 0.0 38-39 0.0 0.0 0.0 0.01670924339575494 0.0 40-41 0.0 0.0 0.0 0.019438520901413646 0.0 42-43 0.0 0.0 0.0 0.022562027380111937 0.0 44-45 0.0 0.0 0.0 0.02635269058241569 0.0 46-47 0.0 0.0 0.0 0.030658883980232754 0.0 48-49 0.0 6.0650611236860046E-5 0.0 0.03681492102077405 0.0 50-51 0.0 6.0650611236860046E-5 0.0 0.045336331899552884 0.0 52-53 0.0 6.0650611236860046E-5 0.0 0.06613949155379588 0.0 54-55 0.0 6.0650611236860046E-5 0.0 0.08921704912942113 0.0 56-57 0.0 6.0650611236860046E-5 0.0 0.1209069935006805 0.0 58-59 0.0 6.0650611236860046E-5 0.0 0.1652729156204436 0.0 60-61 0.0 6.0650611236860046E-5 0.0 0.22189026121005248 0.0 62-63 0.0 6.0650611236860046E-5 0.0 0.26822732819501355 0.0 64-65 0.0 6.0650611236860046E-5 0.0 0.3187189620496995 0.0 66-67 0.0 6.0650611236860046E-5 0.0 0.39562393709803806 0.0 68-69 0.0 6.0650611236860046E-5 0.0 0.4951819154433439 0.0 70-71 0.0 6.0650611236860046E-5 0.0 0.5830343258199356 0.0 72-73 0.0 6.0650611236860046E-5 0.0 0.6727972304504884 0.0 74-75 0.0 6.0650611236860046E-5 0.0 0.8180554443627683 0.0 76-77 0.0 6.0650611236860046E-5 0.0 0.9385985341960277 0.0 78-79 0.0 6.0650611236860046E-5 0.0 1.0434937663301769 0.0 80-81 0.0 6.0650611236860046E-5 0.0 1.1599429399049483 0.0 82-83 0.0 6.0650611236860046E-5 0.0 1.3431077858402656 0.0 84-85 0.0 6.0650611236860046E-5 0.0 1.5384330793285734 0.0 86-87 0.0 6.0650611236860046E-5 0.0 1.7742729811231037 0.0 88-89 0.0 6.0650611236860046E-5 0.0 2.069065277039862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5150 0.0 51.668617 1 GTATCAA 8760 0.0 40.41087 1 ACCGTCG 60 0.0039517 31.666252 8 ATCAACG 11350 0.0 30.889742 3 TATCAAC 11500 0.0 30.569452 2 TCAACGC 11565 0.0 30.356564 4 CAACGCA 11710 0.0 29.858963 5 AACGCAG 12070 0.0 28.962238 6 ACGCAGA 13530 0.0 25.69811 7 CGCAGAG 13540 0.0 25.64405 8 GAGTACT 9040 0.0 24.117495 12-13 TAGGACG 675 0.0 23.929243 4 GCAGAGT 14710 0.0 23.410639 9 GTACATG 8780 0.0 23.163023 1 GTACTTT 9495 0.0 22.810324 14-15 GTGTAGC 1145 0.0 21.994616 1 TACATGG 9155 0.0 21.794392 2 AGTACTT 9265 0.0 21.711805 12-13 GTGGTAT 2355 0.0 20.984007 1 CAGAGTA 14465 0.0 20.868202 10-11 >>END_MODULE