FastQCFastQC Report
Thu 26 May 2016
SRR937661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620292
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.5367551034011153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.3975209406699533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.2628538559716397No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23720.14639336613400547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.1025741039269465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA405.388531E-447.500548
GGTATCA42050.044.4435771
GTATCAA76250.042.2212641
ATCAACG95700.033.279023
TCAACGC97400.032.6981744
TATCAAC99150.032.3178182
CAACGCA98700.032.1711775
AACGCAG102250.031.0321376
GCGCGAC650.005844441229.23119
ACGCAGA116950.026.8066317
CGCAGAG117450.026.6925148
GTACATG94250.026.1355041
GTGTAGC11400.025.8624291
TACATGG95850.025.3456632
GCAGAGT125650.024.5725049
TAGCCCT16500.024.199314
ACATGGG96550.023.9271873
GTAGCCC10400.023.767183
GAGTACT63650.023.50700412-13
AGTACTT67300.023.11433412-13
Produced by FastQC (version 0.11.5)