##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937661_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1620292 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.701462452446844 33.0 31.0 34.0 30.0 34.0 2 31.825223478237255 33.0 31.0 34.0 30.0 34.0 3 31.844814391480053 33.0 31.0 34.0 30.0 34.0 4 35.45357133158715 37.0 35.0 37.0 33.0 37.0 5 35.46208337756404 37.0 35.0 37.0 33.0 37.0 6 35.515949594270666 37.0 35.0 37.0 33.0 37.0 7 35.46439839238853 37.0 35.0 37.0 33.0 37.0 8 35.445677075490096 37.0 35.0 37.0 33.0 37.0 9 37.0658078914171 39.0 37.0 39.0 33.0 39.0 10-11 36.95208363677658 39.0 37.0 39.0 33.0 39.0 12-13 36.84229694400763 39.0 37.0 39.0 33.0 39.0 14-15 37.97378065188251 40.0 38.0 41.0 33.0 41.0 16-17 37.909235804410564 40.0 38.0 41.0 33.0 41.0 18-19 37.87917116174121 40.0 38.0 41.0 33.0 41.0 20-21 37.81394927580955 40.0 38.0 41.0 33.0 41.0 22-23 37.61774174037766 40.0 37.0 41.0 32.0 41.0 24-25 37.459439718273 40.0 37.0 41.0 32.0 41.0 26-27 37.424192984968144 39.5 37.0 41.0 32.0 41.0 28-29 37.484045468347674 40.0 37.0 41.0 32.0 41.0 30-31 37.308871795947894 39.5 37.0 41.0 32.0 41.0 32-33 37.07287822195012 39.0 36.0 41.0 31.0 41.0 34-35 37.07274552981808 39.0 36.0 41.0 31.0 41.0 36-37 36.987689564597 39.0 36.0 41.0 31.0 41.0 38-39 36.864435854771855 39.0 36.0 41.0 31.0 41.0 40-41 36.630725202617796 39.0 35.5 41.0 30.0 41.0 42-43 36.37810839033952 39.0 35.0 41.0 30.0 41.0 44-45 36.121441073584265 39.0 35.0 41.0 29.5 41.0 46-47 35.75421035220812 39.0 35.0 40.5 28.5 41.0 48-49 35.40086293087913 39.0 35.0 40.0 26.5 41.0 50-51 34.485619567337245 38.0 34.0 39.5 25.0 40.5 52-53 34.58870654178383 38.0 34.5 39.5 25.5 40.5 54-55 34.9591160111881 38.5 35.0 40.0 26.0 41.0 56-57 34.78745960604632 38.0 34.0 40.0 24.5 41.0 58-59 34.57367036311973 38.0 34.0 40.0 24.0 41.0 60-61 34.278497949752264 38.0 34.0 40.0 23.0 41.0 62-63 33.933409533590236 38.0 33.5 40.0 22.0 41.0 64-65 33.5954056429335 37.0 33.0 40.0 21.5 41.0 66-67 33.2043452661619 37.0 32.5 39.5 19.5 41.0 68-69 32.73274755414456 36.0 32.0 39.0 18.5 40.5 70-71 32.30278770740089 36.0 31.5 39.0 16.5 40.0 72-73 31.83829180172463 35.0 31.0 38.5 12.5 40.0 74-75 31.38071285916366 35.0 31.0 37.5 8.0 39.5 76-77 30.867350452881333 35.0 30.5 37.0 7.0 39.0 78-79 30.311354064576015 34.5 30.0 36.5 2.0 39.0 80-81 29.75818988182377 34.0 29.0 36.0 2.0 38.0 82-83 29.206541166653913 34.0 29.0 35.5 2.0 37.0 84-85 28.698888225085355 34.0 28.5 35.0 2.0 37.0 86-87 28.189934592036497 34.0 27.5 35.0 2.0 36.0 88-89 27.677963601622423 33.0 26.0 35.0 2.0 36.0 90-91 27.153145852722844 33.0 26.0 35.0 2.0 35.5 92-93 26.56818678361678 32.5 25.0 35.0 2.0 35.0 94-95 25.914260207419403 32.0 23.5 34.5 2.0 35.0 96-97 25.15966566520109 32.0 19.5 34.0 2.0 35.0 98-99 24.2039690993969 31.5 7.0 34.0 2.0 35.0 100-101 21.80998918713417 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46015898807657507 1101 2 0.5023639692143327 1101 3 0.4070787534391833 1101 4 0.31736551583551886 1101 5 0.26326383296922984 1101 6 0.34712107156072136 1101 7 0.39753445283149347 1101 8 0.22725115859980605 1101 9 0.398168093052071 1101 10-11 0.2576696759011128 1101 12-13 0.4406658694626273 1101 14-15 0.6857824157302517 1101 16-17 0.68538231710518 1101 18-19 0.7505171161530058 1101 20-21 0.7712551946333406 1101 22-23 0.747529473208516 1101 24-25 0.7972103098340142 1101 26-27 0.7649159075256762 1101 28-29 0.7205841479636987 1101 30-31 0.749650474295521 1101 32-33 0.650117716775064 1101 34-35 0.7761085611385639 1101 36-37 0.7476618482420747 1101 38-39 0.6591292432121136 1101 40-41 0.5261891880079119 1101 42-43 0.7512744106767499 1101 44-45 0.8048322513167392 1101 46-47 0.6666150234807446 1101 48-49 0.5987880770155201 1101 50-51 0.618088788203579 1101 52-53 0.6669579726853314 1101 54-55 0.560249697639172 1101 56-57 0.6982718847313549 1101 58-59 0.6821865737223831 1101 60-61 0.747962043937811 1101 62-63 0.8848993054580276 1101 64-65 0.8325864646804035 1101 66-67 0.8102124917589748 1101 68-69 0.8067418730097629 1101 70-71 1.1392885231315972 1101 72-73 1.1038803132224224 1101 74-75 1.1058685324125967 1101 76-77 1.1881735853756688 1101 78-79 1.072507517838222 1101 80-81 0.9711837696787455 1101 82-83 0.960499631768652 1101 84-85 1.3315050441325837 1101 86-87 1.3366629117662683 1101 88-89 1.3796868209852668 1101 90-91 1.4981886299150027 1101 92-93 1.591607472304542 1101 94-95 1.8183323140469092 1101 96-97 1.9471471104226978 1101 98-99 2.2966097792859124 1101 100-101 2.9612417598527294 1102 1 0.3686316233062392 1102 2 0.3119616475522058 1102 3 0.38855837924902303 1102 4 0.3580110871305422 1102 5 0.23589624297337508 1102 6 0.21664426757676125 1102 7 0.16118690625835086 1102 8 0.32883515509475103 1102 9 0.4268205790134374 1102 10-11 0.4702980516269051 1102 12-13 0.5144992364149417 1102 14-15 0.5844311493789505 1102 16-17 0.6674944136502248 1102 18-19 0.5942122548475979 1102 20-21 0.6588979397645502 1102 22-23 0.7181506002470712 1102 24-25 0.7593807082081625 1102 26-27 0.7453943382347745 1102 28-29 0.7394613172016733 1102 30-31 0.6791649076854611 1102 32-33 0.6191160228119443 1102 34-35 0.8051555821710323 1102 36-37 0.9020838294197802 1102 38-39 0.8052560106489821 1102 40-41 0.8542107215108601 1102 42-43 0.8496223086225214 1102 44-45 1.0805680190984646 1102 46-47 1.2387765507804431 1102 48-49 1.24717056998265 1102 50-51 1.1840273326966795 1102 52-53 1.0466607808467145 1102 54-55 0.9520043701360237 1102 56-57 1.1311794498708494 1102 58-59 1.080899725686706 1102 60-61 1.2536547143481087 1102 62-63 1.241781979533883 1102 64-65 1.1553486873508803 1102 66-67 1.3748505237252147 1102 68-69 1.3415893621052817 1102 70-71 1.5171628922333582 1102 72-73 1.3358480650232032 1102 74-75 1.354372270626964 1102 76-77 1.2998192022190018 1102 78-79 1.3590063498495724 1102 80-81 1.4361107080301991 1102 82-83 1.529597710280914 1102 84-85 1.6778536968788238 1102 86-87 1.7833703116304918 1102 88-89 1.922822149334312 1102 90-91 1.671838936064674 1102 92-93 1.8634612232073735 1102 94-95 2.063891245960871 1102 96-97 2.223492140994285 1102 98-99 2.3578950222861117 1102 100-101 2.9483559277611278 1103 1 0.41927245126335677 1103 2 0.384349096616976 1103 3 0.31040162824882245 1103 4 0.2852075213557299 1103 5 0.3707546522726801 1103 6 0.37174971533396217 1103 7 0.30329240746928576 1103 8 0.39859925968313803 1103 9 0.46308564371300065 1103 10-11 0.47653918550382457 1103 12-13 0.4707249509473641 1103 14-15 0.5562408870033835 1103 16-17 0.5573007878016512 1103 18-19 0.38936646885164805 1103 20-21 0.5846262468027632 1103 22-23 0.5872368704515551 1103 24-25 0.7467027175748981 1103 26-27 0.4978166144589693 1103 28-29 0.4365238292801621 1103 30-31 0.5055356874108767 1103 32-33 0.46084858147741414 1103 34-35 0.48406870341642616 1103 36-37 0.31501120225131274 1103 38-39 0.523251002445889 1103 40-41 0.8206582278944694 1103 42-43 0.6295623582111389 1103 44-45 0.4674360829633386 1103 46-47 0.4022030991910128 1103 48-49 0.3527729318150463 1103 50-51 0.22349838750319861 1103 52-53 0.2509839538599934 1103 54-55 0.4264035296789572 1103 56-57 0.31478416366726236 1103 58-59 0.396817628984806 1103 60-61 0.43290294934714524 1103 62-63 0.5375924408395534 1103 64-65 0.7021163815626608 1103 66-67 0.523570306949793 1103 68-69 0.5383562339520225 1103 70-71 0.5376730875039399 1103 72-73 0.38234047253797954 1103 74-75 0.39465746682270364 1103 76-77 0.6295468760361302 1103 78-79 0.7183305302600154 1103 80-81 0.6140966126934231 1103 82-83 0.6754373655454913 1103 84-85 0.8535826206908119 1103 86-87 1.085493863754042 1103 88-89 1.1693918591628112 1103 90-91 1.4145355121478325 1103 92-93 1.2601070018020302 1103 94-95 1.3672717830474816 1103 96-97 1.501815832097531 1103 98-99 1.551962892072833 1103 100-101 2.0413962524308467 1104 1 0.3968515341055969 1104 2 0.41208736119734013 1104 3 0.33769960291521883 1104 4 0.34611993753130577 1104 5 0.30586269264387766 1104 6 0.32777152661893183 1104 7 0.35902295004193263 1104 8 0.33716188245628587 1104 9 0.4436790190432234 1104 10-11 0.4554222038605218 1104 12-13 0.5435082605727857 1104 14-15 0.7166672908508431 1104 16-17 0.7967930996623807 1104 18-19 0.777439692451189 1104 20-21 0.732944678015933 1104 22-23 0.6658116858684764 1104 24-25 0.5375904097783817 1104 26-27 0.6454746108011733 1104 28-29 0.590151140802206 1104 30-31 0.6910078136724778 1104 32-33 0.6934994654440843 1104 34-35 0.6492282238391027 1104 36-37 0.8273308527288563 1104 38-39 0.6975042030148799 1104 40-41 0.6893586952791466 1104 42-43 0.7909624801375728 1104 44-45 0.7872220343282379 1104 46-47 0.7686005116410541 1104 48-49 0.7546729521361115 1104 50-51 0.5996414478567829 1104 52-53 0.6671002581341909 1104 54-55 0.9120348376786822 1104 56-57 0.8228533230522075 1104 58-59 0.7563651464270578 1104 60-61 0.8391465990118476 1104 62-63 0.9531414484938168 1104 64-65 0.9912175127690546 1104 66-67 0.8747692502539905 1104 68-69 1.0614545202082084 1104 70-71 1.0779934830874964 1104 72-73 1.1658363744549298 1104 74-75 1.1762736694915397 1104 76-77 1.2257248208982823 1104 78-79 1.3316035777435893 1104 80-81 1.3121762712993963 1104 82-83 1.2828342361002534 1104 84-85 1.3195109550401973 1104 86-87 1.555926702607934 1104 88-89 1.6949371412870384 1104 90-91 1.4135702611825813 1104 92-93 1.6011284744024543 1104 94-95 1.7576395944679213 1104 96-97 1.8621931266853515 1104 98-99 2.288999740951784 1104 100-101 2.9902718197274645 1105 1 0.264634454168327 1105 2 0.16885994191712683 1105 3 0.17379844326880445 1105 4 0.23462820693173114 1105 5 0.23454100842058523 1105 6 0.23804330728000878 1105 7 0.3408644889624384 1105 8 0.17696428470634373 1105 9 0.33202655880286613 1105 10-11 0.4311109483187465 1105 12-13 0.41572899381900896 1105 14-15 0.49571209413850426 1105 16-17 0.5411964776171345 1105 18-19 0.4215070936084402 1105 20-21 0.4719011956626886 1105 22-23 0.5889705537574841 1105 24-25 0.5510715585446277 1105 26-27 0.40709879379206626 1105 28-29 0.4850251814097746 1105 30-31 0.509002234798487 1105 32-33 0.4089826753785317 1105 34-35 0.5010825573176092 1105 36-37 0.48465226734113287 1105 38-39 0.4217663223488586 1105 40-41 0.5964619483194511 1105 42-43 0.570896481276641 1105 44-45 0.5123336676444978 1105 46-47 0.5589669831986868 1105 48-49 0.554696961874015 1105 50-51 0.42431851289365596 1105 52-53 0.42105408764749797 1105 54-55 0.43413516328582347 1105 56-57 0.29405021247499974 1105 58-59 0.3558480197465741 1105 60-61 0.4791155937456537 1105 62-63 0.4450319800422946 1105 64-65 0.4079057164099211 1105 66-67 0.4553051699386259 1105 68-69 0.559160433623191 1105 70-71 0.6584025739240715 1105 72-73 0.5874541521901655 1105 74-75 0.6378269798840073 1105 76-77 0.8409984840504379 1105 78-79 0.8508564361786632 1105 80-81 0.9371210329489053 1105 82-83 0.9549892416918944 1105 84-85 0.9385158989722768 1105 86-87 1.0465784593071312 1105 88-89 1.0361827202248328 1105 90-91 1.246145812090404 1105 92-93 1.591062331961691 1105 94-95 1.6652615296368474 1105 96-97 1.6717827436303203 1105 98-99 1.994201438703488 1105 100-101 2.144437868203209 1106 1 0.12157837096058444 1106 2 0.171887058067977 1106 3 0.14012067535917083 1106 4 -0.0294261411040182 1106 5 0.025319437272244727 1106 6 0.07522563620118916 1106 7 -0.029069316196782324 1106 8 0.03696970240140729 1106 9 0.1388656021016601 1106 10-11 -0.1014566533615806 1106 12-13 -0.2509928142486544 1106 14-15 -0.12113001862838502 1106 16-17 -0.0750988337284042 1106 18-19 0.08438913126561687 1106 20-21 0.14619247141246916 1106 22-23 0.10992344748595428 1106 24-25 0.01090932960759261 1106 26-27 0.04976720988781835 1106 28-29 0.18086458449047882 1106 30-31 0.1808997866880162 1106 32-33 0.07581518557444866 1106 34-35 0.10569795548045846 1106 36-37 0.11809110315729754 1106 38-39 0.18308079271046296 1106 40-41 0.3317465324610325 1106 42-43 0.3063393610750964 1106 44-45 0.27514639067364044 1106 46-47 0.43927051669321315 1106 48-49 0.4331799904829694 1106 50-51 0.3354458798854765 1106 52-53 0.35198812008155045 1106 54-55 0.3793109825864107 1106 56-57 0.31141786682270123 1106 58-59 0.42528299440668604 1106 60-61 0.31746071433969547 1106 62-63 0.5766666646963827 1106 64-65 0.5727231543433504 1106 66-67 0.5591357990370227 1106 68-69 0.4271959488786976 1106 70-71 0.5600137511489507 1106 72-73 0.4712961701893299 1106 74-75 0.8426852518070085 1106 76-77 0.806894890340665 1106 78-79 0.9003178535516838 1106 80-81 1.015535459784445 1106 82-83 0.7745873342606764 1106 84-85 0.9088028606936618 1106 86-87 1.0821186647266714 1106 88-89 1.101763248055935 1106 90-91 1.274101034756832 1106 92-93 1.2824463937066426 1106 94-95 1.5878945797572328 1106 96-97 1.7115868679555213 1106 98-99 2.120580917212596 1106 100-101 2.5519898499809663 1107 1 0.10719101918192564 1107 2 0.13528626755414308 1107 3 0.16815756600475495 1107 4 0.07044210659031336 1107 5 0.07014157165959745 1107 6 0.07780798139223322 1107 7 0.12313094730782836 1107 8 0.2627668038506812 1107 9 0.289010529637892 1107 10-11 0.3007172596818961 1107 12-13 0.20043011065252614 1107 14-15 0.4251282158907159 1107 16-17 0.45424240474326893 1107 18-19 0.550304809789985 1107 20-21 0.40479589697240925 1107 22-23 0.48238721560190356 1107 24-25 0.5567986576708392 1107 26-27 0.561677618319969 1107 28-29 0.47535733811366754 1107 30-31 0.41278384465903173 1107 32-33 0.4410194016482407 1107 34-35 0.5032473625950828 1107 36-37 0.42701073425084246 1107 38-39 0.5845695937644777 1107 40-41 0.32244481968105987 1107 42-43 0.4499098485586259 1107 44-45 0.4827221482494011 1107 46-47 0.4283350753229911 1107 48-49 0.4708611499032642 1107 50-51 0.42905589306070624 1107 52-53 0.44561130848733654 1107 54-55 0.33052310379852656 1107 56-57 0.466174125057222 1107 58-59 0.4524371446043176 1107 60-61 0.534101030545223 1107 62-63 0.4806192338663422 1107 64-65 0.3880459474922304 1107 66-67 0.4457102390897205 1107 68-69 0.4553118909076801 1107 70-71 0.7262983361291901 1107 72-73 0.6534964336939346 1107 74-75 0.7116575838228236 1107 76-77 0.8598066162958702 1107 78-79 0.9656933476228318 1107 80-81 0.7203444189412309 1107 82-83 0.8246136847218075 1107 84-85 1.0373008844090776 1107 86-87 1.18034000860019 1107 88-89 1.1853073850783353 1107 90-91 1.2308862784985983 1107 92-93 1.1612606229556555 1107 94-95 1.2917680975437946 1107 96-97 1.5693997796814791 1107 98-99 1.9295795996895393 1107 100-101 2.5658106668452625 1108 1 0.10696499902939038 1108 2 0.13057064841109067 1108 3 0.20562569493644745 1108 4 0.13401166624070981 1108 5 0.19744176279322545 1108 6 0.19970373006079 1108 7 0.12858809069516042 1108 8 0.13960660418099025 1108 9 0.33306107798727425 1108 10-11 0.19194738085690233 1108 12-13 0.2943086387380376 1108 14-15 0.3800344119604162 1108 16-17 0.4187542717291066 1108 18-19 0.4748259551072138 1108 20-21 0.45686811950391615 1108 22-23 0.4153290033507915 1108 24-25 0.48399289026004766 1108 26-27 0.5862356913961122 1108 28-29 0.39450280379311664 1108 30-31 0.27253722661082236 1108 32-33 0.3663986070519414 1108 34-35 0.390630873144552 1108 36-37 0.5806530360192923 1108 38-39 0.6944745836510933 1108 40-41 0.5773393250532166 1108 42-43 0.7971909263295984 1108 44-45 0.7832278173098786 1108 46-47 0.8644454132398209 1108 48-49 0.9961427472607838 1108 50-51 1.2300393961875002 1108 52-53 1.0960267921667608 1108 54-55 1.2360489068381142 1108 56-57 1.1542614898255366 1108 58-59 1.3041265431828748 1108 60-61 1.2123743330745 1108 62-63 1.148068063868756 1108 64-65 1.154650820471872 1108 66-67 1.1030408631502908 1108 68-69 1.277666360279916 1108 70-71 1.3086869497609115 1108 72-73 1.4473580752163322 1108 74-75 1.3763052855801412 1108 76-77 1.2402254401745871 1108 78-79 1.4366100467636578 1108 80-81 1.5714374063783652 1108 82-83 1.8383062750463672 1108 84-85 1.8347246629167486 1108 86-87 1.9346256698668682 1108 88-89 1.9210955793294815 1108 90-91 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8504.0 15 11048.0 16 14539.0 17 19140.0 18 16233.0 19 11016.0 20 10498.0 21 11072.0 22 12186.0 23 13563.0 24 15347.0 25 17745.0 26 20638.0 27 24125.0 28 28238.0 29 34343.0 30 41907.0 31 51589.0 32 64947.0 33 83766.0 34 111797.0 35 154546.0 36 218418.0 37 304456.0 38 255197.0 39 29691.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83994046646162 19.835886539575935 15.045776932487382 22.278396061475064 2 14.44835029064819 20.738991053693315 42.28261118508078 22.530047470577713 3 18.63598103655291 25.836953965365716 30.04716105313974 25.479903944941633 4 11.700515420216922 17.661109815014328 39.03041096856797 31.607963796200778 5 12.750470139126529 38.57572751670626 34.61634852733057 14.057453816836643 6 29.906435692862654 37.593652446978474 18.71849000679759 13.781421853361284 7 27.463749965211097 33.741275716728666 21.1900587236648 17.604915594395433 8 25.158565898713288 36.05139675309493 20.344620264517733 18.445417083674055 9 26.333535393522528 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20.75799830450759 34-35 20.653063698543928 28.277727420467663 30.79591249146838 20.27329638952003 36-37 20.531022376852174 28.133506034598177 30.569515299614945 20.7659562889347 38-39 20.44953978942278 28.415989422593192 30.609515028000384 20.524955759983648 40-41 21.044496649317033 28.00106732099339 30.515640952228416 20.43879507746116 42-43 20.564835584845937 28.09059609267784 30.506124943024144 20.83844337945208 44-45 20.82540566030443 28.363954018723376 30.090710205091337 20.719930115880857 46-47 20.792517020475902 28.344793699494016 29.68599204860758 21.1766972314225 48-49 20.83468117282137 28.488914728921905 29.564939061161503 21.111465037095222 50-51 21.030652988132832 28.842077264953037 29.055605899872884 21.07166384704125 52-53 20.97075293656924 28.922419139752947 29.062794155722234 21.044033767955582 54-55 21.06499470424974 28.68450471205807 29.077488036243032 21.17301254744915 56-57 21.191754070147226 28.890648963694126 28.674493641008425 21.243103325150223 58-59 21.122036198605414 28.8799887468679 28.891674691736828 21.10630036278985 60-61 21.230403552256625 28.799879794128895 29.03076905477931 20.938947598835163 62-63 21.23797953912793 28.67703737555165 29.109605454006328 20.975377631314096 64-65 21.227390839937733 28.6831850144609 28.97781322797206 21.11161091762931 66-67 21.145966248151495 28.842826008519122 28.820905208462477 21.190302534866902 68-69 21.34822393410538 28.729089079247 28.924961080681637 20.99772590596598 70-71 21.259351987170707 28.63696806249426 28.931235474836242 21.172444475498793 72-73 21.139386249353823 28.6795605254501 28.880802916638842 21.30025030855724 74-75 21.06513723883654 28.86747621219265 28.729054748672446 21.338331800298363 76-77 20.95491257550803 28.73053118937015 28.990197794395506 21.324358440726314 78-79 20.850467171221275 28.70527716537332 28.89395168435535 21.55030397905005 80-81 20.832166028746304 28.9793336066836 28.697912704788514 21.49058765978158 82-83 21.016606607145178 28.882002366099833 28.82971440445218 21.27167662230281 84-85 21.055232038626624 28.73804280812282 28.716430351927468 21.49029480132309 86-87 20.825884312873082 28.85505747005602 28.638188167358543 21.680870049712357 88-89 21.14729431962038 28.540303663542133 28.695901698653113 21.616500318184375 90-91 21.051866675901923 28.63601056777028 28.83891818883458 21.473204567493223 92-93 20.970745721131536 28.542425058540406 29.019004103471424 21.467825116856638 94-95 20.82991531772935 28.709290313284512 28.82360380311117 21.637190565874963 96-97 20.96436454070378 28.491177779090016 28.888209409606358 21.656248270599843 98-99 20.958418760858482 28.339789804228094 28.877291392708543 21.824500042204882 100-101 21.13299001614149 27.954092738924867 29.344344958650755 21.568572286282883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2692.0 1 1560.5 2 466.5 3 504.5 4 473.5 5 481.0 6 743.0 7 1479.0 8 2295.0 9 2544.5 10 2257.0 11 1977.5 12 1934.5 13 1998.5 14 2127.0 15 2225.5 16 2298.0 17 2384.5 18 2564.0 19 2857.0 20 3185.0 21 3630.5 22 4284.5 23 5181.0 24 6277.0 25 7669.0 26 9699.0 27 12271.5 28 15071.5 29 18588.0 30 22824.0 31 27186.5 32 32472.5 33 38464.0 34 44417.5 35 50174.0 36 55820.0 37 60645.5 38 62876.0 39 63234.0 40 63284.0 41 63410.5 42 63245.5 43 63494.5 44 63738.5 45 63377.0 46 62519.0 47 61051.0 48 60501.0 49 58860.0 50 55524.0 51 51041.0 52 45830.5 53 41512.0 54 37238.0 55 33076.0 56 28978.0 57 24802.0 58 21275.0 59 17960.0 60 14619.0 61 12035.0 62 9529.0 63 6637.5 64 4611.5 65 3475.5 66 2602.5 67 1999.5 68 1489.0 69 1031.5 70 723.5 71 561.0 72 444.5 73 353.5 74 258.0 75 205.0 76 161.0 77 99.5 78 70.0 79 53.0 80 36.0 81 24.5 82 12.5 83 11.0 84 8.5 85 5.5 86 4.0 87 3.0 88 5.5 89 6.0 90 3.5 91 2.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3127831279794012 2 0.2940827949530085 3 0.2809370162908908 4 0.2671740649216314 5 0.26421163592735136 6 0.2186642901402957 7 0.20965356861602724 8 0.2230462163610016 9 0.22292278181957328 10-11 0.2247742999409983 12-13 0.22073181870922032 14-15 0.21656590293601402 16-17 0.21690534792494195 18-19 0.21628817521780025 20-21 0.20258694111925504 22-23 0.19397738185462865 24-25 0.19539687908105455 26-27 0.21033245859388305 28-29 0.2262555144381383 30-31 0.22033065644957822 32-33 0.22542233128349706 34-35 0.21678191338351357 36-37 0.20005653301997417 38-39 0.20122916116354336 40-41 0.19496485818605536 42-43 0.20854265774317224 44-45 0.20548765284282094 46-47 0.20628997736210508 48-49 0.2164424683945857 50-51 0.22508288629456913 52-53 0.2411602353156098 54-55 0.21165937991423767 56-57 0.18123276545215306 58-59 0.18296084903214976 60-61 0.18925601064499487 62-63 0.19783471127426414 64-65 0.18512095350714564 66-67 0.19172470147356155 68-69 0.1953043031749833 70-71 0.18857712066713903 72-73 0.19058293196534945 74-75 0.19314419869998742 76-77 0.2105176104060256 78-79 0.21517726434494522 80-81 0.20483962150032214 82-83 0.20274123429604046 84-85 0.19558203089319703 86-87 0.19681637630748036 88-89 0.20397557971032382 90-91 0.20366699335675298 92-93 0.200365119373545 94-95 0.2004268366442592 96-97 0.18765136160642648 98-99 0.19629177950640994 100-101 0.187095906169999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1620292.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.37605090755712 #Duplication Level Percentage of deduplicated Percentage of total 1 79.83730463368913 54.58959605955273 2 12.856284970035597 17.58123991186431 3 3.7434328767712013 7.67883470853392 4 1.4881089192585244 4.070040448768427 5 0.7253506088547113 2.4798305078438645 6 0.3790134840294014 1.5549267167186962 7 0.23861231161736718 1.1420757296423285 8 0.14588363787876824 0.7979957640142628 9 0.10544145879254252 0.6488703498749376 >10 0.4431676360740428 5.241034924642651 >50 0.02539512033008855 1.181418694318077 >100 0.011011656441079298 1.308420651701514 >500 4.512210124619705E-4 0.20134479145516967 >1k 3.6097680996957643E-4 0.5902740652957871 >5k 1.8048840498478822E-4 0.9340966757733249 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8697 0.5367551034011153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6441 0.3975209406699533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.2628538559716397 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2372 0.14639336613400547 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1662 0.1025741039269465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.171727071416757E-5 0.0 3 0.0 0.0 0.0 6.171727071416757E-5 0.0 4 0.0 0.0 0.0 1.851518121425027E-4 0.0 5 0.0 0.0 0.0 1.851518121425027E-4 0.0 6 0.0 0.0 0.0 1.851518121425027E-4 0.0 7 0.0 0.0 0.0 2.468690828566703E-4 0.0 8 0.0 0.0 0.0 2.468690828566703E-4 0.0 9 0.0 0.0 0.0 6.171727071416757E-4 0.0 10-11 0.0 0.0 0.0 6.171727071416757E-4 0.0 12-13 0.0 0.0 0.0 6.171727071416757E-4 0.0 14-15 0.0 0.0 0.0 7.097486132129271E-4 0.0 16-17 0.0 0.0 0.0 8.023245192841785E-4 0.0 18-19 0.0 0.0 0.0 8.64041789998346E-4 0.0 20-21 0.0 0.0 0.0 8.949004253554298E-4 0.0 22-23 0.0 0.0 0.0 0.0011726281435691837 0.0 24-25 0.0 0.0 0.0 0.0013577799557116864 0.0 26-27 0.0 0.0 0.0 0.001851518121425027 0.0 28-29 0.0 0.0 0.0 0.002406973557852535 0.0 30-31 0.0 0.0 0.0 0.004351067585348814 0.0 32-33 0.0 0.0 0.0 0.007868952016056365 0.0 34-35 0.0 0.0 0.0 0.010522794656765571 0.0 36-37 0.0 0.0 0.0 0.012621181861047269 0.0 38-39 0.0 0.0 0.0 0.014472699982472295 0.0 40-41 0.0 0.0 0.0 0.01721911852925275 0.0 42-43 0.0 0.0 0.0 0.02095301340745989 0.0 44-45 0.0 0.0 0.0 0.02447089783816744 0.0 46-47 0.0 0.0 0.0 0.029593431307443352 0.0 48-49 0.0 0.0 0.0 0.0363514724506447 0.0 50-51 0.0 0.0 0.0 0.04499189035062816 0.0 52-53 0.0 0.0 0.0 0.06514257923880387 0.0 54-55 0.0 0.0 0.0 0.0881014039444742 0.0 56-57 0.0 0.0 0.0 0.11646048983763421 0.0 58-59 0.0 0.0 0.0 0.1587059616414819 0.0 60-61 0.0 0.0 0.0 0.21761509653815486 0.0 62-63 0.0 0.0 0.0 0.26229840053521214 0.0 64-65 0.0 0.0 0.0 0.3119190861894029 0.0 66-67 0.0 0.0 0.0 0.3923675485653203 0.0 68-69 0.0 0.0 0.0 0.49444791432655344 0.0 70-71 0.0 0.0 0.0 0.5819012869285289 0.0 72-73 0.0 0.0 0.0 0.6679351623040786 0.0 74-75 0.0 0.0 0.0 0.8147605493330832 0.0 76-77 0.0 0.0 0.0 0.9341217508942832 0.0 78-79 0.0 0.0 0.0 1.0374981793405138 0.0 80-81 0.0 0.0 0.0 1.1574765536088556 0.0 82-83 0.0 0.0 0.0 1.3454673602042102 0.0 84-85 0.0 0.0 0.0 1.5349085226613475 0.0 86-87 0.0 0.0 0.0 1.7705450622480392 0.0 88-89 0.0 0.0 0.0 2.0548148111574953 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCGA 40 5.388531E-4 47.50054 8 GGTATCA 4205 0.0 44.443577 1 GTATCAA 7625 0.0 42.221264 1 ATCAACG 9570 0.0 33.27902 3 TCAACGC 9740 0.0 32.698174 4 TATCAAC 9915 0.0 32.317818 2 CAACGCA 9870 0.0 32.171177 5 AACGCAG 10225 0.0 31.032137 6 GCGCGAC 65 0.0058444412 29.2311 9 ACGCAGA 11695 0.0 26.806631 7 CGCAGAG 11745 0.0 26.692514 8 GTACATG 9425 0.0 26.135504 1 GTGTAGC 1140 0.0 25.862429 1 TACATGG 9585 0.0 25.345663 2 GCAGAGT 12565 0.0 24.572504 9 TAGCCCT 1650 0.0 24.19931 4 ACATGGG 9655 0.0 23.927187 3 GTAGCCC 1040 0.0 23.76718 3 GAGTACT 6365 0.0 23.507004 12-13 AGTACTT 6730 0.0 23.114334 12-13 >>END_MODULE