FastQCFastQC Report
Thu 26 May 2016
SRR937661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620292
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.6787048260437007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91400.5640958543274917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.3720934251357163No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.14164113628901456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.050.47571
GTATCAA84900.042.6598241
ATCAACG107700.033.3433883
TCAACGC109850.032.690794
TATCAAC111800.032.205572
CAACGCA111650.032.163755
AACGCAG115500.031.0887476
ACGCAGA131650.027.1692547
CGCAGAG131800.027.0302128
ATTCGCG651.0031423E-525.57593580-81
GCAGAGT142000.024.7875449
TAGCCCT15550.024.7433364
GAGTACT84100.023.75054712-13
CGCGGAA701.7815235E-523.74871682-83
ATAGCCC8150.023.3134213
GTACATG86050.023.2542841
AGTACTT86450.022.85767212-13
TACATGG88850.022.186782
GTACTTT91150.021.78126114-15
ATGGGCG1754.050129E-621.7147875
Produced by FastQC (version 0.11.5)