##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1620292 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21239258109032 33.0 31.0 34.0 31.0 34.0 2 32.41430124940443 34.0 31.0 34.0 31.0 34.0 3 32.478861834780396 34.0 31.0 34.0 31.0 34.0 4 36.093286271857174 37.0 35.0 37.0 35.0 37.0 5 36.0194785878101 37.0 35.0 37.0 35.0 37.0 6 36.066280645710776 37.0 35.0 37.0 35.0 37.0 7 36.02393025454671 37.0 35.0 37.0 35.0 37.0 8 35.98674930197767 37.0 35.0 37.0 35.0 37.0 9 37.672774413500775 39.0 37.0 39.0 35.0 39.0 10-11 37.59779934727814 39.0 37.0 39.0 35.0 39.0 12-13 37.499524468429144 39.0 37.0 39.0 35.0 39.0 14-15 38.71082743110501 40.0 38.0 41.0 34.5 41.0 16-17 38.67537949949762 40.0 38.0 41.0 34.5 41.0 18-19 38.682989238976674 40.0 38.0 41.0 34.0 41.0 20-21 38.60649685365354 40.0 38.0 41.0 34.0 41.0 22-23 38.558557655039955 40.0 38.0 41.0 34.0 41.0 24-25 38.58511305369649 40.0 38.0 41.0 34.0 41.0 26-27 38.42084667455002 40.0 38.0 41.0 34.0 41.0 28-29 38.374991976754806 40.0 38.0 41.0 34.0 41.0 30-31 38.3467933557655 40.0 38.0 41.0 34.0 41.0 32-33 38.18893847528717 40.0 37.5 41.0 34.0 41.0 34-35 38.04233773912357 40.0 37.0 41.0 33.5 41.0 36-37 37.878200349072884 40.0 37.0 41.0 33.0 41.0 38-39 37.736888474423125 40.0 37.0 41.0 33.0 41.0 40-41 37.54434571052625 40.0 37.0 41.0 33.0 41.0 42-43 37.305491232444524 40.0 37.0 41.0 32.5 41.0 44-45 36.95727807086624 40.0 36.0 41.0 31.5 41.0 46-47 36.563933846491864 40.0 36.0 41.0 30.5 41.0 48-49 36.19053664401231 39.0 36.0 41.0 30.0 41.0 50-51 35.94308217284292 39.0 35.5 41.0 29.0 41.0 52-53 35.6940619345155 39.0 35.0 40.5 28.0 41.0 54-55 35.489227867569554 39.0 35.0 40.0 28.0 41.0 56-57 35.23009648878103 39.0 35.0 40.0 27.0 41.0 58-59 35.00586252354513 38.0 35.0 40.0 26.0 41.0 60-61 34.69950786648333 38.0 34.0 40.0 26.0 41.0 62-63 34.31966244355955 38.0 34.0 40.0 25.5 41.0 64-65 33.957898020850564 37.5 34.0 40.0 24.0 41.0 66-67 33.56265167019278 37.0 33.0 39.5 23.0 41.0 68-69 33.10358410706219 36.0 32.5 39.0 22.0 40.0 70-71 32.69855001444184 36.0 32.0 39.0 21.0 40.0 72-73 32.226168184500075 35.0 31.5 38.0 19.5 40.0 74-75 31.74297132862472 35.0 31.0 37.5 18.0 39.0 76-77 30.431930170611224 34.0 29.5 36.0 17.5 38.5 78-79 31.03427869791371 34.5 31.0 36.0 17.5 39.0 80-81 30.840104746551855 34.5 31.0 36.0 14.5 38.5 82-83 30.418205483949805 34.0 30.5 36.0 9.5 37.0 84-85 29.972209021583765 34.0 30.0 35.0 7.0 37.0 86-87 29.614098878473754 34.0 30.0 35.0 2.0 36.0 88-89 29.18862032275664 34.0 29.0 35.0 2.0 36.0 90-91 28.673441268610844 34.0 29.0 35.0 2.0 35.5 92-93 28.21687510646229 34.0 29.0 35.0 2.0 35.0 94-95 27.72184303816843 33.0 27.0 35.0 2.0 35.0 96-97 27.18844165125792 33.0 26.5 35.0 2.0 35.0 98-99 26.369543884682514 33.0 25.5 34.5 2.0 35.0 100-101 23.92355667990708 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32531231371675773 1101 2 0.34933141350292374 1101 3 0.30892706333271036 1101 4 0.1870864939015746 1101 5 0.1950439600393068 1101 6 0.10914201004164426 1101 7 0.14410406275612786 1101 8 0.284947545691864 1101 9 0.2780913335248272 1101 10-11 0.33991454055488646 1101 12-13 0.390244808470797 1101 14-15 0.4644347788764591 1101 16-17 0.49136633496780746 1101 18-19 0.4890711932462679 1101 20-21 0.45469092157275526 1101 22-23 0.42126055369627124 1101 24-25 0.37863555108643254 1101 26-27 0.34067501077667117 1101 28-29 0.48437067731668293 1101 30-31 0.5000146443737847 1101 32-33 0.47090493952754997 1101 34-35 0.5061833536687388 1101 36-37 0.37641722539816413 1101 38-39 0.4480015252918932 1101 40-41 0.42660491574386583 1101 42-43 0.4817867178653046 1101 44-45 0.4345236750613779 1101 46-47 0.4713700932154339 1101 48-49 0.46214896033851716 1101 50-51 0.448143263307486 1101 52-53 0.43604586088738273 1101 54-55 0.3912666455390692 1101 56-57 0.43301745074455766 1101 58-59 0.4963345931191441 1101 60-61 0.5806353858759508 1101 62-63 0.5012370392143239 1101 64-65 0.5458791905752278 1101 66-67 0.6164656795581536 1101 68-69 0.5727699755524256 1101 70-71 0.4554405703467381 1101 72-73 0.5147960205206488 1101 74-75 0.4874150708823848 1101 76-77 0.41340988544486024 1101 78-79 0.5169369443438789 1101 80-81 0.6179782575504262 1101 82-83 0.7634095355043762 1101 84-85 0.6028201004018889 1101 86-87 0.8004915862544664 1101 88-89 0.9456044492552884 1101 90-91 0.8113897817794644 1101 92-93 1.072868557982325 1101 94-95 1.1737879348263611 1101 96-97 1.111991696434334 1101 98-99 1.3630924160851734 1101 100-101 1.642780530368718 1102 1 0.3003336238161154 1102 2 0.2772770331659089 1102 3 0.3209392355747056 1102 4 0.14668211172094203 1102 5 0.20615470965594795 1102 6 0.21110600952976455 1102 7 0.2062913790717431 1102 8 0.22948749697686566 1102 9 0.21630831263117045 1102 10-11 0.2864375782363453 1102 12-13 0.2940743363517839 1102 14-15 0.32989701824798345 1102 16-17 0.42753537556870214 1102 18-19 0.4154558074629833 1102 20-21 0.4027361401937739 1102 22-23 0.34863573507612955 1102 24-25 0.302515876171185 1102 26-27 0.18267813842687275 1102 28-29 0.374041406458268 1102 30-31 0.2875684618639056 1102 32-33 0.2736870733137593 1102 34-35 0.1981980852916223 1102 36-37 0.2826174340527814 1102 38-39 0.30507702052125296 1102 40-41 0.2825944092684125 1102 42-43 0.2808197924408944 1102 44-45 0.25796174780419534 1102 46-47 0.13401159119069916 1102 48-49 0.25187196158133673 1102 50-51 0.14422545847546786 1102 52-53 0.04320063185532774 1102 54-55 0.1986708476954533 1102 56-57 -0.04628865337886623 1102 58-59 0.02866391169482796 1102 60-61 0.012776655025213302 1102 62-63 0.17798788348466843 1102 64-65 0.13411905228190335 1102 66-67 0.07979257105991877 1102 68-69 0.20478717979752759 1102 70-71 0.14244824198251393 1102 72-73 0.25478265968482106 1102 74-75 0.30136326294006466 1102 76-77 0.33560947811288244 1102 78-79 0.19669289219323716 1102 80-81 0.18916418355829379 1102 82-83 0.32929277134264723 1102 84-85 0.27594811933263586 1102 86-87 0.2621318891289981 1102 88-89 0.3454124956885316 1102 90-91 0.5078372309659933 1102 92-93 0.5670312174827217 1102 94-95 0.703638124285618 1102 96-97 0.8263233618321557 1102 98-99 0.6934759544943248 1102 100-101 1.4732978940328856 1103 1 0.2588888929145199 1103 2 0.2428812042013888 1103 3 0.19015728977678492 1103 4 0.1123014965096587 1103 5 0.12022619784883659 1103 6 0.1719075687338787 1103 7 0.09854264448595274 1103 8 0.10559697021093939 1103 9 0.175642361422085 1103 10-11 0.24249758621328255 1103 12-13 0.2778241975776865 1103 14-15 0.35431098036162467 1103 16-17 0.2336476037256574 1103 18-19 0.2967739352667351 1103 20-21 0.29398679294602204 1103 22-23 0.3492284546784248 1103 24-25 0.2875605511519552 1103 26-27 0.31401888126921307 1103 28-29 0.2641263567668588 1103 30-31 0.3203783598495207 1103 32-33 0.2264808529445972 1103 34-35 0.29803710733511934 1103 36-37 0.3661140068464803 1103 38-39 0.33101153559314866 1103 40-41 0.3602404715790115 1103 42-43 0.24797514953235122 1103 44-45 0.443509093415436 1103 46-47 0.35490171910958423 1103 48-49 0.26956206153980844 1103 50-51 0.24238294960419893 1103 52-53 0.1821252250930172 1103 54-55 0.3430047525724902 1103 56-57 0.32832802171704145 1103 58-59 0.26652950435274647 1103 60-61 0.486872361868528 1103 62-63 0.38274773233397497 1103 64-65 0.3418141275488651 1103 66-67 0.4538952680060717 1103 68-69 0.4980760643004629 1103 70-71 0.5758138531436501 1103 72-73 0.37612097655029686 1103 74-75 0.5313140032741899 1103 76-77 0.28496597746938335 1103 78-79 0.5595986779424749 1103 80-81 0.6658273140552886 1103 82-83 0.66912751708794 1103 84-85 0.5583071556852808 1103 86-87 0.6892767199479479 1103 88-89 0.8856481443906361 1103 90-91 0.6620497958814333 1103 92-93 0.705447383534672 1103 94-95 0.964104364144827 1103 96-97 1.208801846706809 1103 98-99 1.3186214103107048 1103 100-101 1.97094161260312 1104 1 0.22090542072051988 1104 2 0.22008773403949533 1104 3 0.1262644495681542 1104 4 0.16406942985653927 1104 5 0.16295125373179076 1104 6 0.17558229609281994 1104 7 0.25879123894507217 1104 8 0.19379042682545133 1104 9 0.1733054380325285 1104 10-11 0.25862743786944975 1104 12-13 0.2375884731314315 1104 14-15 0.31690327190128187 1104 16-17 0.38312602951987174 1104 18-19 0.4073036379016983 1104 20-21 0.2717775536315159 1104 22-23 0.3439619099382014 1104 24-25 0.18691197024021733 1104 26-27 0.3166944892079826 1104 28-29 0.3363931724547271 1104 30-31 0.3203444913946001 1104 32-33 0.3798964866233874 1104 34-35 0.38556166196494246 1104 36-37 0.38603712545380375 1104 38-39 0.40949321275903117 1104 40-41 0.4089602439829889 1104 42-43 0.4749276659585533 1104 44-45 0.28390399959981494 1104 46-47 0.33490239647867526 1104 48-49 0.514363253709412 1104 50-51 0.3560539514330898 1104 52-53 0.619553254626311 1104 54-55 0.6197438977326897 1104 56-57 0.5871253528827935 1104 58-59 0.5015257788227103 1104 60-61 0.5859375925126997 1104 62-63 0.491236860559944 1104 64-65 0.6062082208903234 1104 66-67 0.6739470867420962 1104 68-69 0.5518253022602337 1104 70-71 0.5076535876149606 1104 72-73 0.48588017183580234 1104 74-75 0.6379064980245772 1104 76-77 0.5559728866341906 1104 78-79 0.689594275043337 1104 80-81 0.6508997925488202 1104 82-83 0.6358432930142435 1104 84-85 0.7222135432758812 1104 86-87 0.832006856099138 1104 88-89 0.7226731393103698 1104 90-91 0.7869989931990524 1104 92-93 0.8297869486637097 1104 94-95 0.8838784615505055 1104 96-97 1.0694585560477243 1104 98-99 1.161734260002504 1104 100-101 1.7267161841671665 1105 1 0.22900850784635196 1105 2 0.19254274170506847 1105 3 0.228144308349421 1105 4 0.1846176974063809 1105 5 0.13464249647901738 1105 6 0.16165784460764598 1105 7 0.15829931850105794 1105 8 0.12614598153015777 1105 9 0.23263661128711988 1105 10-11 0.24646283635931354 1105 12-13 0.23661332679965597 1105 14-15 0.25717924828399674 1105 16-17 0.3236819865671663 1105 18-19 0.34188308460885963 1105 20-21 0.24455541649363965 1105 22-23 0.3534660968855121 1105 24-25 0.36819167331713487 1105 26-27 0.22112376736837547 1105 28-29 0.19487630740859885 1105 30-31 0.2969186447920933 1105 32-33 0.31200376787553097 1105 34-35 0.37773636192297744 1105 36-37 0.2858277780879419 1105 38-39 0.39854305729398476 1105 40-41 0.4388659279893119 1105 42-43 0.5901620440744395 1105 44-45 0.5744629080102399 1105 46-47 0.5953518172936114 1105 48-49 0.6581596479413392 1105 50-51 0.6133117123216323 1105 52-53 0.8079519247580365 1105 54-55 0.8321834147301459 1105 56-57 0.7056686477845986 1105 58-59 0.6945768342142173 1105 60-61 0.919844048659435 1105 62-63 0.9022989285242531 1105 64-65 0.8516962247956954 1105 66-67 0.8856409857161438 1105 68-69 0.8180546088175191 1105 70-71 0.7747495579673256 1105 72-73 0.8120502676673595 1105 74-75 0.943336487702787 1105 76-77 0.8360550541790843 1105 78-79 0.9550556289373056 1105 80-81 0.9963566476081738 1105 82-83 0.8368085881744811 1105 84-85 1.0574297665201762 1105 86-87 1.242329886782148 1105 88-89 1.2292064203168316 1105 90-91 1.2841303878856394 1105 92-93 1.242102548292955 1105 94-95 1.6041017999729448 1105 96-97 1.7945121591835083 1105 98-99 1.886789059636854 1105 100-101 2.281438046110374 1106 1 -0.3723241948203082 1106 2 -0.2396832348847866 1106 3 -0.2986590251264829 1106 4 -0.19258554750782508 1106 5 -0.21246109136018987 1106 6 -0.291407807624978 1106 7 -0.2331476002477757 1106 8 -0.12916122106899053 1106 9 -0.5195977686006543 1106 10-11 -0.5748804920343531 1106 12-13 -0.6374969677434876 1106 14-15 -0.6178124700226988 1106 16-17 -0.5783141321491172 1106 18-19 -0.5324593070099866 1106 20-21 -0.6038415831432218 1106 22-23 -0.6494603809669286 1106 24-25 -0.4242961389656017 1106 26-27 -0.5022922067810001 1106 28-29 -0.6908451308133223 1106 30-31 -0.5524501434137647 1106 32-33 -0.5823943689741 1106 34-35 -0.7861088681152069 1106 36-37 -0.9822093306072972 1106 38-39 -0.8001328781599639 1106 40-41 -0.8744236109111583 1106 42-43 -0.9842662674916696 1106 44-45 -0.7545543003001356 1106 46-47 -0.6807642404694221 1106 48-49 -0.8193910926307453 1106 50-51 -0.8966640590080246 1106 52-53 -1.0497880829072415 1106 54-55 -1.0913123991359654 1106 56-57 -1.2203961683114954 1106 58-59 -1.1388275749173715 1106 60-61 -1.5634898297980087 1106 62-63 -1.5734344521090762 1106 64-65 -2.0237362053927725 1106 66-67 -1.860966035985669 1106 68-69 -1.780316811918496 1106 70-71 -1.9001222749663924 1106 72-73 -1.8201479914578051 1106 74-75 -1.8247211679783746 1106 76-77 -1.6735473071308569 1106 78-79 -1.682010378883973 1106 80-81 -1.665817148023958 1106 82-83 -1.9669792277144573 1106 84-85 -1.7869372982548235 1106 86-87 -1.7606370560527793 1106 88-89 -1.8707372641686852 1106 90-91 -2.0762819859286097 1106 92-93 -1.8681622226836296 1106 94-95 -1.647485769184435 1106 96-97 -2.1096292325938393 1106 98-99 -2.2414339867012103 1106 100-101 -1.522875185561503 1107 1 0.13958621361184242 1107 2 0.18374232506251076 1107 3 0.11807351769301277 1107 4 0.1376647818729424 1107 5 0.15217658979923243 1107 6 0.09786755469386321 1107 7 0.13067321661652187 1107 8 0.06456736918133998 1107 9 0.11887390465363268 1107 10-11 0.13062675434245818 1107 12-13 0.17220000195348462 1107 14-15 0.1602639463145934 1107 16-17 0.1001048401828939 1107 18-19 0.22681605530885207 1107 20-21 0.22665907561830778 1107 22-23 0.1367188021687724 1107 24-25 0.11347724706865137 1107 26-27 0.30508592233625365 1107 28-29 0.25628266892316987 1107 30-31 0.19121257068373154 1107 32-33 0.2577900842538341 1107 34-35 0.19397018326819193 1107 36-37 0.14288948362194986 1107 38-39 0.38669189127350023 1107 40-41 0.33241433375287244 1107 42-43 0.37269025424745905 1107 44-45 0.45306098296732245 1107 46-47 0.179472913680776 1107 48-49 0.22637965835740204 1107 50-51 0.18573383295508705 1107 52-53 0.12603536900316215 1107 54-55 0.208832528400265 1107 56-57 0.31877379216281554 1107 58-59 0.19959139741465037 1107 60-61 0.23052861552478277 1107 62-63 0.28827832786456753 1107 64-65 0.2664087221434599 1107 66-67 0.37049641460721006 1107 68-69 0.3356120418364483 1107 70-71 0.25334281067260633 1107 72-73 0.31965438008369773 1107 74-75 0.43074655270673645 1107 76-77 0.3368215993250061 1107 78-79 0.3652755236193208 1107 80-81 0.4833001115280844 1107 82-83 0.47415384211426215 1107 84-85 0.6987412261193526 1107 86-87 0.8218530625242941 1107 88-89 0.8627278214703118 1107 90-91 0.837868211699849 1107 92-93 0.8589655770528637 1107 94-95 0.9732256932661585 1107 96-97 0.9201204380253962 1107 98-99 0.9783815600708579 1107 100-101 1.4516307432922524 1108 1 0.11320160821927061 1108 2 0.12010262122684878 1108 3 0.09057625480077292 1108 4 0.08427986101710871 1108 5 0.07740325943496629 1108 6 0.14492428241195654 1108 7 0.14577945351087607 1108 8 0.06213013709315618 1108 9 0.22573059154706243 1108 10-11 0.1366701027066881 1108 12-13 0.19482502746889452 1108 14-15 0.204229134015236 1108 16-17 0.21173114610803623 1108 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64-65 -0.2770131067651036 2316 66-67 -0.4098414775890902 2316 68-69 -0.16457299436628858 2316 70-71 -0.10456279404715474 2316 72-73 -0.052123576473022126 2316 74-75 0.001677097088609969 2316 76-77 -0.0865144156924238 2316 78-79 0.058976247674028315 2316 80-81 0.015901345102772524 2316 82-83 -0.12951202004717288 2316 84-85 -0.05728719973208385 2316 86-87 0.0429418262679242 2316 88-89 0.09312471071676143 2316 90-91 -0.046602155690870006 2316 92-93 -0.06071530253952062 2316 94-95 -0.1507673343551872 2316 96-97 -0.2381606024680316 2316 98-99 -0.26430816907905097 2316 100-101 0.4570209283284541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 12.0 5 32.0 6 57.0 7 134.0 8 386.0 9 799.0 10 1354.0 11 2396.0 12 3705.0 13 5391.0 14 7164.0 15 10258.0 16 14360.0 17 20386.0 18 17871.0 19 10354.0 20 8165.0 21 7971.0 22 8472.0 23 9178.0 24 10635.0 25 12169.0 26 15066.0 27 18314.0 28 22663.0 29 28277.0 30 35820.0 31 45164.0 32 57534.0 33 75444.0 34 103239.0 35 147657.0 36 222929.0 37 355453.0 38 314713.0 39 26762.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.984893069754406 20.320018967013944 15.766824272344587 21.928263690887064 2 14.936381837347836 21.238702653595773 41.496964744626275 22.327950764430117 3 18.772665667669777 25.732337134294315 30.709711582850495 24.785285615185412 4 12.086786933203316 18.209579766584994 38.749606551913544 30.954026748298148 5 12.669398940832274 38.424889207105565 34.8920463663634 14.013665485698768 6 29.955896838347655 37.991670637144416 18.17888380612877 13.873548718379155 7 27.239596319675712 33.96326094308927 21.160136567976636 17.63700616925838 8 25.361107750948598 36.097937902550896 20.206419583630606 18.334534762869904 9 26.179293608806315 16.866157458038426 21.204819871973694 35.74972906118156 10-11 24.052639894537528 27.802766414942493 27.90651314701301 20.23808054350697 12-13 25.353535727599734 25.334032010783826 28.46005811613846 20.852374145477977 14-15 22.00069493646824 26.70216232629674 27.71614622549516 23.580996511739862 16-17 20.353923860637465 29.91352793200238 28.537572240065373 21.194975967294784 18-19 20.11547918523328 27.887905389892687 31.952543121857047 20.04407230301699 20-21 21.636440502751057 26.740635135176838 31.986996114881023 19.63592824719108 22-23 20.753454671135056 27.33868429335851 31.65540311182704 20.252457923679387 24-25 20.326521392440373 27.183155875607607 32.32466740562812 20.165655326323897 26-27 20.336924911372904 27.615548500498683 31.78713203709007 20.260394551038345 28-29 20.11620934964054 27.636705218990343 31.899062221592693 20.34802320977642 30-31 20.578698160578462 27.308318500615936 32.052339948601855 20.06064339020374 32-33 20.239166841275168 27.456757009464038 31.918022158358543 20.386053990902255 34-35 20.51642543442787 27.654922692946705 31.85789968721687 19.970752185408553 36-37 20.434126793586568 27.521142859258884 31.647202008039315 20.39752833911523 38-39 20.393083468905605 27.69932826922555 31.681573444786494 20.22601481708235 40-41 20.9658879281907 27.393157342367097 31.555618232892048 20.08533649655016 42-43 20.505154916005562 27.51289616369802 31.534573262216288 20.44737565808013 44-45 20.72774536935318 27.745060766824746 31.014101162012775 20.513092701809306 46-47 20.775082515990945 27.834550809360287 30.478426110849156 20.91194056379961 48-49 20.81566162148551 27.965514857815755 30.33518032552157 20.883643195177164 50-51 21.102029757599247 28.286753251882995 29.696746018618864 20.914470971898893 52-53 21.04666936576864 28.443175674508055 29.657894996704297 20.852259963019012 54-55 21.12202615331064 28.209699239396357 29.64453320759468 21.023741399698327 56-57 21.236110528225776 28.42064887069738 29.193565110486258 21.149675490590585 58-59 21.165907132788412 28.418735635305243 29.436237418934365 20.979119812971984 60-61 21.290946323255312 28.268515798386957 29.60031278312798 20.840225095229748 62-63 21.359730221466254 28.165787401283225 29.64761907113039 20.82686330612013 64-65 21.354576829361623 28.23898408435023 29.429325084614376 20.977114001673772 66-67 21.224908843591155 28.430338482199506 29.336625744001697 21.00812693020764 68-69 21.497174583346705 28.23824347710166 29.395812606616584 20.86876933293505 70-71 21.31976828867883 28.168564678465362 29.476446220804647 21.03522081205116 72-73 21.312454792099203 28.291227754009775 29.309871307147105 21.08644614674392 74-75 21.222594445939375 28.426542870050586 29.110431946834275 21.24043073717577 76-77 20.999424795036944 28.277989399441584 29.611267598679746 21.11131820684173 78-79 20.915551024136388 28.200163921071013 29.42361623707332 21.460668817719274 80-81 20.910181621584258 28.518686755226835 29.29215845045214 21.278973172736766 82-83 21.07303079882663 28.42863217611179 29.331279790210264 21.167057234851313 84-85 21.048189551370157 28.26989506193031 29.269469829147766 21.41244555755177 86-87 20.712748072569635 28.368004038778196 29.22417687676048 21.695071011891685 88-89 21.089871455268558 28.05093156048416 29.30292811419176 21.55626887005552 90-91 20.935599673109824 28.15372479501468 29.487826797587335 21.42284873428816 92-93 20.78636411748895 28.097376060365825 29.608434971172738 21.507824850972487 94-95 20.63047895070765 28.206798527672788 29.474131823152867 21.688590698466694 96-97 20.852167387112942 28.00408815201211 29.530541408585613 21.61320305228934 98-99 20.79848101172075 27.80198470766635 29.47875348163198 21.920780798980925 100-101 20.55778399897043 27.909600056046287 29.786965473203797 21.74565047177949 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 698.0 1 479.5 2 399.5 3 541.5 4 431.5 5 377.5 6 759.5 7 1777.0 8 2825.5 9 3235.0 10 2741.5 11 1991.0 12 1935.0 13 2085.5 14 2191.5 15 2353.0 16 2454.0 17 2627.0 18 2858.0 19 3137.0 20 3556.5 21 4032.0 22 4615.5 23 5494.0 24 6657.5 25 8063.5 26 10076.0 27 12614.5 28 15378.5 29 18829.5 30 23058.0 31 27580.0 32 32660.0 33 38335.0 34 44119.5 35 49910.5 36 56035.0 37 60883.5 38 62909.5 39 63324.0 40 63285.0 41 63297.5 42 63268.5 43 63847.5 44 63944.5 45 63470.5 46 62706.5 47 61169.5 48 60848.5 49 59031.0 50 55508.5 51 50987.5 52 45353.0 53 41031.5 54 36792.0 55 32526.0 56 28558.0 57 24471.5 58 20886.0 59 17554.0 60 14191.0 61 11327.5 62 8925.5 63 6391.0 64 4372.5 65 3247.0 66 2433.0 67 1872.5 68 1383.0 69 999.0 70 717.0 71 531.0 72 453.0 73 380.5 74 270.0 75 189.5 76 136.0 77 80.5 78 56.0 79 39.5 80 30.5 81 20.5 82 9.0 83 4.5 84 2.5 85 2.0 86 2.5 87 2.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03937561871563891 2 0.0 3 0.0 4 1.2343454142833515E-4 5 0.009381025148553471 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005431119822846746 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.32020894999173E-4 22-23 0.0013577799557116866 24-25 0.0 26-27 0.0012343454142833515 28-29 0.0016355076739254404 30-31 0.0 32-33 3.085863535708379E-5 34-35 0.0 36-37 3.085863535708379E-4 38-39 0.0 40-41 3.085863535708379E-5 42-43 0.020582709783174884 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.851518121425027E-4 84-85 1.851518121425027E-4 86-87 0.0 88-89 0.0 90-91 0.010862239645693493 92-93 0.0023143976517812836 94-95 0.0 96-97 0.0 98-99 1.851518121425027E-4 100-101 0.012497747319618934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1620292.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.99489256233967 #Duplication Level Percentage of deduplicated Percentage of total 1 78.85422315251228 52.82830208189318 2 13.474715078853347 18.054741780318366 3 3.9552447788159117 7.949435970435808 4 1.5399729868730034 4.126812992178487 5 0.7733354793909855 2.590476367822226 6 0.40585539841125684 1.6314143287444627 7 0.2347358100003699 1.100827026005599 8 0.16346850953325803 0.8761244186805135 9 0.10261487368576921 0.6187205194088549 >10 0.4548419400753358 5.320023203851621 >50 0.027805640362438044 1.259938484890348 >100 0.01198774219747901 1.4449820342015118 >500 6.454050015196632E-4 0.28099068201441946 >1k 2.7660214350842707E-4 0.3009423176026053 >5k 1.8440142900561806E-4 0.936985635409261 >10k+ 9.220071450280903E-5 0.6792821565426622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10997 0.6787048260437007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9140 0.5640958543274917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6029 0.3720934251357163 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2295 0.14164113628901456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.171727071416757E-5 0.0 4 0.0 0.0 0.0 6.171727071416757E-5 0.0 5 0.0 0.0 0.0 6.171727071416757E-5 0.0 6 0.0 0.0 0.0 6.171727071416757E-5 0.0 7 0.0 0.0 0.0 6.171727071416757E-5 0.0 8 0.0 0.0 0.0 6.171727071416757E-5 0.0 9 0.0 0.0 0.0 1.851518121425027E-4 0.0 10-11 0.0 0.0 0.0 1.851518121425027E-4 0.0 12-13 0.0 0.0 0.0 2.468690828566703E-4 0.0 14-15 0.0 0.0 0.0 2.7772771821375406E-4 0.0 16-17 0.0 0.0 0.0 3.703036242850054E-4 0.0 18-19 0.0 0.0 0.0 4.6287953035625677E-4 0.0 20-21 0.0 0.0 0.0 5.554554364275081E-4 0.0 22-23 0.0 0.0 0.0 8.640417899983459E-4 0.0 24-25 0.0 0.0 0.0 9.874763314266812E-4 0.0 26-27 0.0 0.0 0.0 0.0014194972264258542 0.0 28-29 0.0 0.0 0.0 0.002005811298210446 0.0 30-31 0.0 0.0 0.0 0.004011622596420892 0.0 32-33 0.0 0.0 0.0 0.007436931121057192 0.0 34-35 0.0 0.0 0.0 0.010152491032480566 0.0 36-37 0.0 0.0 0.0 0.012281736872119347 0.0 38-39 0.0 0.0 0.0 0.01410239635818729 0.0 40-41 0.0 0.0 0.0 0.01678709763425358 0.0 42-43 0.0 0.0 0.0 0.020397557971032383 0.0 44-45 0.0 0.0 0.0 0.02388458376638285 0.0 46-47 0.0 0.0 0.0 0.02925398631851543 0.0 48-49 0.0 0.0 0.0 0.03604288609707386 0.0 50-51 0.0 0.0 0.0 0.044930173079913996 0.0 52-53 0.0 0.0 0.0 0.06514257923880387 0.0 54-55 0.0 0.0 0.0 0.08803968667376004 0.0 56-57 0.0 0.0 0.0 0.11612104484870628 0.0 58-59 0.0 0.0 0.0 0.1586751030061248 0.0 60-61 0.0 0.0 0.0 0.21742994472601235 0.0 62-63 0.0 0.0 0.0 0.2616195105573563 0.0 64-65 0.0 0.0 0.0 0.3112401962115471 0.0 66-67 0.0 0.0 0.0 0.3924909831067487 0.0 68-69 0.0 0.0 0.0 0.4941701866083398 0.0 70-71 0.0 0.0 0.0 0.5816544178456722 0.0 72-73 0.0 0.0 0.0 0.6674722827737223 0.0 74-75 0.0 0.0 0.0 0.8159331774766524 0.0 76-77 0.0 0.0 0.0 0.9376396353249908 0.0 78-79 0.0 0.0 0.0 1.0414789433015779 0.0 80-81 0.0 0.0 0.0 1.1629076734317025 0.0 82-83 0.0 0.0 0.0 1.353552322667766 0.0 84-85 0.0 0.0 0.0 1.5445055582574003 0.0 86-87 0.0 0.0 0.0 1.7842771549819416 0.0 88-89 0.0 0.0 0.0 2.070892160178536 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 50.4757 1 GTATCAA 8490 0.0 42.659824 1 ATCAACG 10770 0.0 33.343388 3 TCAACGC 10985 0.0 32.69079 4 TATCAAC 11180 0.0 32.20557 2 CAACGCA 11165 0.0 32.16375 5 AACGCAG 11550 0.0 31.088747 6 ACGCAGA 13165 0.0 27.169254 7 CGCAGAG 13180 0.0 27.030212 8 ATTCGCG 65 1.0031423E-5 25.575935 80-81 GCAGAGT 14200 0.0 24.787544 9 TAGCCCT 1555 0.0 24.743336 4 GAGTACT 8410 0.0 23.750547 12-13 CGCGGAA 70 1.7815235E-5 23.748716 82-83 ATAGCCC 815 0.0 23.313421 3 GTACATG 8605 0.0 23.254284 1 AGTACTT 8645 0.0 22.857672 12-13 TACATGG 8885 0.0 22.18678 2 GTACTTT 9115 0.0 21.781261 14-15 ATGGGCG 175 4.050129E-6 21.714787 5 >>END_MODULE