FastQCFastQC Report
Thu 26 May 2016
SRR937660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529508
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80950.5292551591753688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.4125509641008743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40900.2674062508989819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20810.13605682350141352No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.10317043127593972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.047.4343451
GTATCAA75200.042.9354361
ATCAACG94400.034.0897563
TCAACGC95000.033.9233784
CAACGCA96250.033.4301385
TATCAAC97150.033.1247862
AACGCAG99300.032.4841426
ACGCAGA113700.028.2447077
CGCAGAG113900.028.195118
GTGTAGC10450.027.7573171
TAGCCCT15350.026.0114084
GCAGAGT122900.025.8194479
GTACATG88000.024.3160921
GAGTACT65100.024.03951312-13
AGTACTT67250.023.8359612-13
TAGGACG5600.023.7663764
TACATGG90600.023.452362
GTACTTT70150.022.20592714-15
ACATGGG88200.022.2042373
GTAGCCC10150.021.5426453
Produced by FastQC (version 0.11.5)