##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937660_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1529508 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.719916469871357 33.0 31.0 34.0 30.0 34.0 2 31.811660350910227 33.0 31.0 34.0 30.0 34.0 3 31.856563679300795 33.0 31.0 34.0 30.0 34.0 4 35.47907823953847 37.0 35.0 37.0 33.0 37.0 5 35.50282051483222 37.0 35.0 37.0 33.0 37.0 6 35.566069611927496 37.0 35.0 37.0 33.0 37.0 7 35.494668873912396 37.0 35.0 37.0 33.0 37.0 8 35.495557394927 37.0 35.0 37.0 33.0 37.0 9 37.06384929009852 39.0 37.0 39.0 33.0 39.0 10-11 36.980343679143886 39.0 37.0 39.0 33.0 39.0 12-13 36.888634776673285 39.0 37.0 39.0 33.0 39.0 14-15 37.98873918933408 40.0 38.0 41.0 33.0 41.0 16-17 37.90263960698473 40.0 38.0 41.0 33.0 41.0 18-19 37.854478695109805 40.0 38.0 41.0 33.0 41.0 20-21 37.89502147095667 40.0 38.0 41.0 33.0 41.0 22-23 37.89378741399195 40.0 38.0 41.0 33.0 41.0 24-25 37.83765858040625 40.0 38.0 41.0 33.0 41.0 26-27 37.747091221490834 40.0 37.5 41.0 33.0 41.0 28-29 37.60547934368437 40.0 37.0 41.0 32.0 41.0 30-31 37.36866364870272 39.5 37.0 41.0 32.0 41.0 32-33 37.2016452349383 39.0 36.0 41.0 32.0 41.0 34-35 37.157468610821255 40.0 36.0 41.0 31.5 41.0 36-37 37.002136634787135 39.5 36.0 41.0 31.0 41.0 38-39 36.837749459303254 39.0 36.0 41.0 31.0 41.0 40-41 36.58979259997332 39.0 35.5 41.0 30.0 41.0 42-43 36.32037393723995 39.0 35.0 41.0 30.0 41.0 44-45 36.03337315005871 39.0 35.0 40.5 29.5 41.0 46-47 35.63137394508561 39.0 35.0 40.0 27.5 41.0 48-49 35.25446908417609 39.0 35.0 40.0 26.0 41.0 50-51 34.396883180735244 38.0 34.0 39.5 24.5 40.5 52-53 34.508112739521465 38.0 34.0 39.5 24.5 40.5 54-55 34.793618274634724 38.0 34.5 40.0 25.0 41.0 56-57 34.572729596706914 38.0 34.0 40.0 24.0 41.0 58-59 34.3095920387471 38.0 34.0 40.0 23.0 41.0 60-61 33.98034204463134 38.0 33.5 40.0 22.5 41.0 62-63 33.6154263331738 37.5 33.0 40.0 21.5 41.0 64-65 33.20222842901116 37.0 32.5 40.0 19.5 41.0 66-67 32.71841010311813 36.5 32.0 39.0 17.5 41.0 68-69 32.2455528182919 36.0 31.0 39.0 13.5 40.0 70-71 31.78139898581766 35.5 31.0 38.5 9.5 40.0 72-73 31.283395052526693 35.0 30.5 38.0 7.5 40.0 74-75 30.71234998443944 35.0 30.0 37.0 4.5 39.0 76-77 30.142978657189108 34.0 29.0 37.0 2.0 39.0 78-79 29.577129377551472 34.0 29.0 36.0 2.0 39.0 80-81 28.98670585573923 34.0 28.5 36.0 2.0 37.5 82-83 28.402546112867668 34.0 27.5 35.0 2.0 37.0 84-85 27.787085781833113 33.0 26.0 35.0 2.0 36.5 86-87 27.15418258681877 33.0 26.0 35.0 2.0 36.0 88-89 26.56742691113744 32.0 25.0 35.0 2.0 35.5 90-91 25.952467067841425 32.0 23.5 34.5 2.0 35.0 92-93 25.23294386168624 31.5 20.0 34.0 2.0 35.0 94-95 24.39022254215081 31.0 14.5 34.0 2.0 35.0 96-97 23.42527237516901 31.0 2.0 34.0 2.0 35.0 98-99 22.182033699725665 30.5 2.0 34.0 2.0 35.0 100-101 18.95954581473258 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13290135863072194 1101 2 0.10887462727606234 1101 3 0.19639413532872751 1101 4 0.14451523459839422 1101 5 0.05158395708268415 1101 6 0.09607369878821004 1101 7 0.078710114926416 1101 8 0.12474545436023021 1101 9 0.308866861924173 1101 10-11 0.2515726358589774 1101 12-13 0.3319099321006931 1101 14-15 0.5404876568692032 1101 16-17 0.5444007811684983 1101 18-19 0.6115651032317118 1101 20-21 0.4774081066344209 1101 22-23 0.4569676199691912 1101 24-25 0.4468147057790972 1101 26-27 0.43534174123870173 1101 28-29 0.4585295249380721 1101 30-31 0.4318361198988043 1101 32-33 0.5196001876532748 1101 34-35 0.6491252934166241 1101 36-37 0.6765604728743213 1101 38-39 0.7134060026012179 1101 40-41 0.7317975095001898 1101 42-43 0.680529070647161 1101 44-45 0.8842639801555805 1101 46-47 0.8568269030197513 1101 48-49 0.9096312569936273 1101 50-51 0.763977318032012 1101 52-53 0.8857266151168801 1101 54-55 0.9336091580855808 1101 56-57 0.9629594742167882 1101 58-59 0.9223430121875325 1101 60-61 0.9217516453996524 1101 62-63 1.0729630501597143 1101 64-65 1.1724796543502194 1101 66-67 1.3410100389331348 1101 68-69 1.2474181939306774 1101 70-71 1.182514989132148 1101 72-73 1.2221657365533858 1101 74-75 1.292517120831672 1101 76-77 1.2487272464204189 1101 78-79 1.4727594293160529 1101 80-81 1.7253851540953455 1101 82-83 1.7126276773777427 1101 84-85 1.9153852561968279 1101 86-87 2.110379339791095 1101 88-89 2.052697735139958 1101 90-91 2.042513318963426 1101 92-93 2.256378971537643 1101 94-95 2.6196862277784696 1101 96-97 2.998215327908415 1101 98-99 3.1001733922294896 1101 100-101 3.7978399589812994 1102 1 0.148252108737978 1102 2 0.14578003896466285 1102 3 0.1301677172677529 1102 4 0.14097437795547307 1102 5 0.0351483086256863 1102 6 0.1783251660963785 1102 7 0.14613463812145255 1102 8 0.13730961048675994 1102 9 0.26124813518603673 1102 10-11 0.27059946182376393 1102 12-13 0.29860355276362327 1102 14-15 0.40095198437823143 1102 16-17 0.49162732726095726 1102 18-19 0.3747077595031172 1102 20-21 0.4801466326551349 1102 22-23 0.347367954198738 1102 24-25 0.4214660446065537 1102 26-27 0.5103996361765226 1102 28-29 0.3822775679656374 1102 30-31 0.49048936347532646 1102 32-33 0.5876280311314233 1102 34-35 0.5974697565042462 1102 36-37 0.538751428460877 1102 38-39 0.6228805569056561 1102 40-41 0.7383062257487367 1102 42-43 0.9433568143955213 1102 44-45 1.0756456644658812 1102 46-47 0.7895688207516613 1102 48-49 0.8258265656505799 1102 50-51 0.8545739672010484 1102 52-53 0.7619884552212781 1102 54-55 0.7672369638281324 1102 56-57 0.884007849901586 1102 58-59 0.9025172248500724 1102 60-61 0.9963928764785095 1102 62-63 1.1803221649904785 1102 64-65 1.1506013201970333 1102 66-67 1.2886795290309152 1102 68-69 1.0810064671453716 1102 70-71 1.2782924210003053 1102 72-73 1.180739016607653 1102 74-75 1.2286952238543378 1102 76-77 1.2820017301952369 1102 78-79 1.4412985002567247 1102 80-81 1.417734486871634 1102 82-83 1.509773249173083 1102 84-85 1.6194056693566665 1102 86-87 1.718977889216859 1102 88-89 1.8265573003270745 1102 90-91 1.9645785341573934 1102 92-93 2.296865446246933 1102 94-95 2.2731180330448346 1102 96-97 2.5546812546878854 1102 98-99 2.8199461700409607 1102 100-101 3.2257124126709655 1103 1 0.17039180206410975 1103 2 0.19296110648471299 1103 3 0.22270807082505328 1103 4 0.23587470675410316 1103 5 0.2556369850465643 1103 6 0.25587906229434765 1103 7 0.3157938552382973 1103 8 0.2736082772939028 1103 9 0.36674464746220536 1103 10-11 0.28440373664498964 1103 12-13 0.4781941128626599 1103 14-15 0.5514238916297955 1103 16-17 0.5777589608502183 1103 18-19 0.5172579901889378 1103 20-21 0.5780164652707356 1103 22-23 0.34917020091624096 1103 24-25 0.501806169014472 1103 26-27 0.4772893064560222 1103 28-29 0.37025402967091026 1103 30-31 0.31596247793149246 1103 32-33 0.3090047711522246 1103 34-35 0.22850676720227625 1103 36-37 0.2665970291878281 1103 38-39 0.3966365874413498 1103 40-41 0.4199242310955569 1103 42-43 0.34219921493284033 1103 44-45 0.6578920746783936 1103 46-47 0.5230235330199307 1103 48-49 0.5539434581742526 1103 50-51 0.4751311250463388 1103 52-53 0.5055948801836436 1103 54-55 0.4571794130926179 1103 56-57 0.6476777957749178 1103 58-59 0.5555589414511175 1103 60-61 0.6324064235090106 1103 62-63 0.7486014375070411 1103 64-65 0.5991245797372358 1103 66-67 0.802240168015274 1103 68-69 0.7828181520078985 1103 70-71 0.8275125668306167 1103 72-73 1.007724209694544 1103 74-75 0.9226923441226447 1103 76-77 0.8893520697489663 1103 78-79 0.8564287247089837 1103 80-81 0.9578839168515678 1103 82-83 1.2032385409434596 1103 84-85 1.4970829199152718 1103 86-87 1.6761080785504383 1103 88-89 1.6863655084403675 1103 90-91 1.7930562711097089 1103 92-93 1.9815706029859896 1103 94-95 2.092068511317347 1103 96-97 2.126879113326421 1103 98-99 2.311609873569072 1103 100-101 2.6246153929298757 1104 1 0.12303927059738129 1104 2 0.17271862635952218 1104 3 0.16744031651753843 1104 4 0.12466169472263999 1104 5 0.09477371349824182 1104 6 0.2034279045071017 1104 7 0.1979429621731228 1104 8 0.16476156987788215 1104 9 0.16829067720956203 1104 10-11 0.2592617501058214 1104 12-13 0.2941061068213102 1104 14-15 0.3220885720547457 1104 16-17 0.3570758556372411 1104 18-19 0.2627926643180558 1104 20-21 0.2286180387311063 1104 22-23 0.2644628576054302 1104 24-25 0.28188071091475564 1104 26-27 0.23706105390257903 1104 28-29 0.34394045795551875 1104 30-31 0.2837777607561165 1104 32-33 0.2778073912727592 1104 34-35 0.3289627600504801 1104 36-37 0.42004535633275 1104 38-39 0.43126733981716114 1104 40-41 0.6164584706310166 1104 42-43 0.6612851471527534 1104 44-45 0.6769290944565398 1104 46-47 0.751398780680482 1104 48-49 0.9704246376250794 1104 50-51 0.8780527345961602 1104 52-53 0.727223185284906 1104 54-55 0.827731644474909 1104 56-57 0.996578422548204 1104 58-59 1.1233824038901545 1104 60-61 1.2337634132787088 1104 62-63 1.2632656684663033 1104 64-65 1.2340927618633728 1104 66-67 1.3182259437484305 1104 68-69 1.2576749261332196 1104 70-71 1.6098583976233236 1104 72-73 1.5349701748312015 1104 74-75 1.4072539415765526 1104 76-77 1.6018824128135627 1104 78-79 1.6522515030348224 1104 80-81 2.076102066341871 1104 82-83 1.9722631673664246 1104 84-85 2.1606706640437174 1104 86-87 2.4310728566350193 1104 88-89 2.48161612686879 1104 90-91 2.2916496252969303 1104 92-93 2.5166917191150304 1104 94-95 2.752252213890042 1104 96-97 2.7869487970314744 1104 98-99 2.964144810437322 1104 100-101 3.768491918758457 1105 1 0.03124874173461123 1105 2 0.07868047186509486 1105 3 0.09190412900416334 1105 4 -0.06431662733553623 1105 5 -0.04597242249504063 1105 6 0.05855604632726141 1105 7 -0.032196290209476786 1105 8 0.0030864262635716955 1105 9 0.254009077946975 1105 10-11 0.1067466479709509 1105 12-13 0.23424568840575688 1105 14-15 0.20097603440228085 1105 16-17 0.13306551869914784 1105 18-19 0.24250477730013387 1105 20-21 0.11972094722945315 1105 22-23 0.2171009989317909 1105 24-25 0.15766133080183664 1105 26-27 0.22838905416594457 1105 28-29 0.30090431159238307 1105 30-31 0.23572745871341994 1105 32-33 0.20054289404628634 1105 34-35 0.4276525366870274 1105 36-37 0.45617567088512345 1105 38-39 0.35420571323081873 1105 40-41 0.4300450424875564 1105 42-43 0.316300537339238 1105 44-45 0.6776418164620353 1105 46-47 0.7211825773654184 1105 48-49 0.5034600432840577 1105 50-51 0.7597807974078776 1105 52-53 0.692628185861011 1105 54-55 0.7234779450691136 1105 56-57 0.5998087157024585 1105 58-59 0.8402276625605154 1105 60-61 0.8359431410287783 1105 62-63 0.9573062919746036 1105 64-65 0.9022367218324305 1105 66-67 1.0921066574580323 1105 68-69 1.1132334993724058 1105 70-71 1.1518373945452787 1105 72-73 1.2673311281997641 1105 74-75 1.3508332709923856 1105 76-77 1.2046448278383366 1105 78-79 1.5767482357064608 1105 80-81 1.600863395000541 1105 82-83 1.5962451106449436 1105 84-85 1.6558174057684028 1105 86-87 1.8535137237526982 1105 88-89 1.8679834667532411 1105 90-91 1.97085559043445 1105 92-93 2.034648034029523 1105 94-95 2.3478775328043326 1105 96-97 2.1766509516575816 1105 98-99 2.178495953590744 1105 100-101 2.897537809496363 1106 1 0.03966760781476708 1106 2 -0.020788875991350153 1106 3 0.1268468744307789 1106 4 0.06445379578973132 1106 5 0.07441055474276936 1106 6 0.049154426441774035 1106 7 0.16969415805193933 1106 8 0.2139944698329117 1106 9 0.24579793425196073 1106 10-11 -0.05248827033654635 1106 12-13 -0.17790956225756105 1106 14-15 -0.2631229042934322 1106 16-17 -0.05148796545110912 1106 18-19 0.1709898003416086 1106 20-21 0.2919114583312492 1106 22-23 0.1903196485617329 1106 24-25 0.006647156803786913 1106 26-27 0.1123804659799319 1106 28-29 0.33561402392305695 1106 30-31 0.3090010936806067 1106 32-33 0.5702547010564771 1106 34-35 0.45549082742854097 1106 36-37 0.29608280675999765 1106 38-39 0.08299446480182127 1106 40-41 0.3038894776223131 1106 42-43 0.5241730411794876 1106 44-45 0.5509171969954849 1106 46-47 0.6331728720170062 1106 48-49 0.6875954643146329 1106 50-51 0.7240577838622855 1106 52-53 0.8112132298492796 1106 54-55 0.9035741257701346 1106 56-57 0.7409848782495843 1106 58-59 0.4115253165194517 1106 60-61 0.7962123073463303 1106 62-63 0.5184656970856238 1106 64-65 0.8475044916969736 1106 66-67 1.008765469076529 1106 68-69 0.8629454809392243 1106 70-71 0.7973635082165593 1106 72-73 0.8935268410890274 1106 74-75 0.846807122409782 1106 76-77 1.026186861073917 1106 78-79 1.1887053645990733 1106 80-81 1.0964741686355062 1106 82-83 1.0769408933568556 1106 84-85 1.2308697727562539 1106 86-87 1.5012183041185665 1106 88-89 1.4578914754112482 1106 90-91 1.65872565048193 1106 92-93 1.7522913450196071 1106 94-95 1.882781875773194 1106 96-97 1.8448633172489828 1106 98-99 2.0946284769813346 1106 100-101 2.417309838542586 1107 1 0.05932291980878901 1107 2 0.10610529928992207 1107 3 0.16835785545788795 1107 4 0.14107707805817427 1107 5 0.24603061950799798 1107 6 0.2718004595716721 1107 7 0.3200735120603255 1107 8 0.23155840473555145 1107 9 0.2652586391965386 1107 10-11 0.25235329357759895 1107 12-13 0.2766055807656542 1107 14-15 0.4413001247263679 1107 16-17 0.36834690398053027 1107 18-19 0.4461083809037376 1107 20-21 0.3767614292699335 1107 22-23 0.3979978048285986 1107 24-25 0.3990969222374261 1107 26-27 0.33002120579809 1107 28-29 0.2960400317281042 1107 30-31 0.2676743406603066 1107 32-33 0.24877034227372974 1107 34-35 0.25153226056675493 1107 36-37 0.25877014847959146 1107 38-39 0.3036645876896884 1107 40-41 0.40691704435955245 1107 42-43 0.3705476415863487 1107 44-45 0.3527968416170637 1107 46-47 0.5337805649634078 1107 48-49 0.37356066338467286 1107 50-51 0.4390103516374282 1107 52-53 0.5613248045576356 1107 54-55 0.6700703166614872 1107 56-57 0.5240862399799795 1107 58-59 0.5558588781917209 1107 60-61 0.7010390811247191 1107 62-63 0.7914059760742873 1107 64-65 0.5932101628058675 1107 66-67 0.8467369370883162 1107 68-69 0.8037096898485956 1107 70-71 0.9238451165530073 1107 72-73 1.107026342894983 1107 74-75 1.2616821068412207 1107 76-77 1.2897185379120444 1107 78-79 1.422799481757707 1107 80-81 1.6942382633754072 1107 82-83 1.8616043010041317 1107 84-85 2.055536605487603 1107 86-87 2.1346123548513276 1107 88-89 2.2170211407909157 1107 90-91 2.12802184760071 1107 92-93 2.2115990109804997 1107 94-95 2.3092411691679686 1107 96-97 2.5911618911685217 1107 98-99 2.65535625545105 1107 100-101 2.952372839331389 1108 1 0.10921235506870985 1108 2 0.2613959830796553 1108 3 0.27572546915862617 1108 4 0.11287919773869959 1108 5 0.2096657012558083 1108 6 0.15481456931156146 1108 7 0.1810337666241253 1108 8 0.3005067070594123 1108 9 0.49302974695498136 1108 10-11 0.34239020011229115 1108 12-13 0.31805839248445267 1108 14-15 0.541023162701471 1108 16-17 0.5066954669649419 1108 18-19 0.4834393651948261 1108 20-21 0.39352423387314417 1108 22-23 0.5670933909602809 1108 24-25 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-1.6393688773504849 2316 78-79 -1.7669946573451867 2316 80-81 -1.768726347054642 2316 82-83 -1.8907873269635296 2316 84-85 -2.0260426696400593 2316 86-87 -2.337724531080031 2316 88-89 -2.5096378750386066 2316 90-91 -2.4607471386337174 2316 92-93 -2.776887665294197 2316 94-95 -3.2937871389294635 2316 96-97 -3.2097710364925156 2316 98-99 -3.3613732785595936 2316 100-101 -3.5794670980384993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5383.0 3 907.0 4 848.0 5 1030.0 6 1196.0 7 1479.0 8 1846.0 9 2224.0 10 2929.0 11 3994.0 12 5484.0 13 7184.0 14 8411.0 15 11239.0 16 14466.0 17 19290.0 18 16017.0 19 11255.0 20 10864.0 21 11703.0 22 12880.0 23 14438.0 24 16477.0 25 19215.0 26 21657.0 27 25642.0 28 30388.0 29 35880.0 30 44093.0 31 53983.0 32 67303.0 33 86176.0 34 113998.0 35 153858.0 36 212615.0 37 278577.0 38 190362.0 39 14217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87992474386543 19.868747843405313 15.009111817548481 22.242215595180774 2 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20.18739054118632 20-21 21.894230303125585 27.221396838123834 31.022814664586175 19.861558194164402 22-23 20.942212870023916 27.915307429680265 30.64040903056363 20.502070669732188 24-25 20.481867330076337 27.734673254905555 31.338180067247208 20.4452793477709 26-27 20.49479282683725 28.151754898595083 30.8486683316343 20.504783942933365 28-29 20.219831267446633 28.224783236686505 30.94201092014704 20.613374575719824 30-31 20.746578169738505 27.965935671556714 31.015508802736154 20.27197735596863 32-33 20.310314304452916 28.04140712683758 30.929547344192564 20.71873122451694 34-35 20.620493910636924 28.325333427681116 30.842158782074968 20.21201387960699 36-37 20.505594975037 28.114512083193848 30.619394297521257 20.760498644247896 38-39 20.376451831281507 28.412055288394615 30.696371367494635 20.51512151282924 40-41 20.97201316811618 28.01843562465367 30.62838061776962 20.381170589460528 42-43 20.588859541552683 28.056610225450427 30.56010832311417 20.794421909882715 44-45 20.807324328051337 28.428250848605725 30.07334875230474 20.6910760710382 46-47 20.791226544949247 28.414179458941007 29.657188230692675 21.137405765417064 48-49 20.786717278617214 28.521474316029416 29.638654391292143 21.053154014061228 50-51 21.04033502891931 28.845566710956316 29.089225298037892 21.024872962086487 52-53 21.009178369647657 28.946739901418116 29.122214996306045 20.921866732628178 54-55 21.07333611773088 28.7078266139269 29.095087176211415 21.123750092130802 56-57 21.15187621659999 28.94178397113956 28.680747133212297 21.225592679048148 58-59 21.10756836967099 28.88355840546512 28.936142904104994 21.072730320758893 60-61 21.17411390147951 28.78896293897216 29.08385699815859 20.95306616138974 62-63 21.17861102797238 28.701996661206795 29.167318859990083 20.952073450830735 64-65 21.232008430751677 28.751633736499056 28.973438743928035 21.04291908882123 66-67 21.153069010574438 28.878533136406652 28.825382582468507 21.143015270550404 68-69 21.370025854178504 28.723975943661138 28.94024066163119 20.965757540529168 70-71 21.188652830652092 28.623324736992917 29.011748331900613 21.17627410045438 72-73 21.120546507720555 28.695290766186375 28.889901279870355 21.294261446222716 74-75 21.057408452494148 28.83219508648132 28.77536735769403 21.3350291033305 76-77 20.900147658621652 28.739254789078768 29.024385290738124 21.33621226156146 78-79 20.82540711918144 28.69102831165088 28.930255866053535 21.55330870311415 80-81 20.722908928934043 28.96777827997982 28.78544618468371 21.52386660640242 82-83 20.9970405394032 28.907481897578496 28.860651880388932 21.23482568262937 84-85 21.094393932695755 28.690738748651246 28.774701227509176 21.440166091143826 86-87 20.73430001683258 28.85012519640739 28.67449654539131 21.74107824136872 88-89 21.159058031177345 28.491042662922528 28.780008037187223 21.569891268712908 90-91 20.952923364019515 28.63263468749253 28.91186595327216 21.502575995215796 92-93 20.930173153183894 28.427703068472354 29.110517337423886 21.531606440919866 94-95 20.81306995360791 28.615887537669416 28.900213457307554 21.670829051415115 96-97 20.9211054654262 28.411248807015177 28.95378260524959 21.71386312230903 98-99 20.929738992363532 28.23164476864607 28.905747460733565 21.932868778256832 100-101 21.156500386554516 27.70674117678263 29.606801525785198 21.529956910877655 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2285.0 1 1345.5 2 425.5 3 456.5 4 441.5 5 477.0 6 713.0 7 1386.5 8 2216.5 9 2492.5 10 2138.5 11 1815.0 12 1814.5 13 1894.5 14 2026.5 15 2077.0 16 2169.0 17 2349.0 18 2434.5 19 2655.5 20 2971.5 21 3452.5 22 4080.0 23 4866.5 24 5998.0 25 7355.5 26 9209.0 27 11683.5 28 14487.0 29 17747.0 30 21633.5 31 26050.5 32 30867.0 33 36435.5 34 42390.0 35 47629.5 36 53096.0 37 57486.5 38 59379.0 39 60072.0 40 60245.0 41 60028.0 42 59530.0 43 59590.5 44 59948.5 45 59739.0 46 58842.0 47 57446.0 48 56811.5 49 55200.0 50 51894.0 51 47717.5 52 42936.5 53 39072.5 54 35277.5 55 31171.0 56 27255.0 57 23425.0 58 20013.0 59 16885.0 60 13786.0 61 11212.0 62 8833.5 63 6261.0 64 4328.5 65 3215.5 66 2419.5 67 1854.5 68 1375.5 69 974.5 70 682.0 71 531.0 72 417.0 73 350.5 74 278.5 75 172.0 76 121.0 77 88.5 78 66.0 79 50.0 80 30.5 81 17.5 82 9.5 83 9.0 84 7.0 85 2.5 86 4.0 87 5.0 88 3.5 89 3.5 90 2.0 91 0.5 92 0.5 93 1.0 94 1.0 95 1.0 96 2.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3343558843758908 2 0.2826399077350364 3 0.28662811832301627 4 0.28277066873792095 5 0.28192072221917114 6 0.21091749765283999 7 0.2020911299581303 8 0.20823689709370594 9 0.21045983414274394 10-11 0.2099367901312056 12-13 0.21248662968745505 14-15 0.20859648985163853 16-17 0.20052199792351524 18-19 0.19375511602423787 20-21 0.1926109572489977 22-23 0.200685449177121 24-25 0.19944321964971745 26-27 0.20607934054611027 28-29 0.212257797932407 30-31 0.2143172837278393 32-33 0.21242124918601277 34-35 0.2118328246730321 36-37 0.199345148897554 38-39 0.186890163372797 40-41 0.1824115990239999 42-43 0.193787806274959 44-45 0.19833175112519844 46-47 0.20470635001582208 48-49 0.21278084194394536 50-51 0.20869456060380198 52-53 0.22003807760403998 54-55 0.20565436728673536 56-57 0.17731191991150094 58-59 0.1596591845220816 60-61 0.17577547812760705 62-63 0.17057772826294468 64-65 0.1776061321679913 66-67 0.17737730041294325 68-69 0.17492553160885724 70-71 0.1762331416377031 72-73 0.18757665863794107 74-75 0.19382049652568015 76-77 0.1970568313470737 78-79 0.19143410822303641 80-81 0.18947269317976762 82-83 0.17714846865789521 84-85 0.17231031155116547 86-87 0.17698501740428949 88-89 0.18662864136702784 90-91 0.18414418231222066 92-93 0.17904450319972176 94-95 0.1790771934504429 96-97 0.18796894164659483 98-99 0.18868812716246008 100-101 0.16714525193722427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1529508.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.08831872835705 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37127981416376 55.527165964069155 2 12.60571603148507 17.418154539648015 3 3.635569924749403 7.535262411609475 4 1.4197698151205917 3.9235803803180804 5 0.6819772766704121 2.3558331728051187 6 0.35744856553971366 1.4817312255001058 7 0.21533850776439123 1.0414162821240536 8 0.14738664327965137 0.8146156309765774 9 0.09819245902350489 0.6105556715163342 >10 0.43058660334922544 5.161573337745078 >50 0.025264187871967998 1.1758333187285008 >100 0.010617101223285274 1.2956555401201428 >500 2.843565863712405E-4 0.1320847138582234 >1k 3.79142115161654E-4 0.5832158956972423 >5k 1.89571057580827E-4 0.9433219152839406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8095 0.5292551591753688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6310 0.4125509641008743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4090 0.2674062508989819 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2081 0.13605682350141352 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.10317043127593972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.538050144229386E-5 0.0 10-11 0.0 0.0 0.0 1.961415043268816E-4 0.0 12-13 0.0 0.0 0.0 1.961415043268816E-4 0.0 14-15 0.0 0.0 0.0 2.6152200576917543E-4 0.0 16-17 0.0 0.0 0.0 2.6152200576917543E-4 0.0 18-19 0.0 0.0 0.0 3.269025072114693E-4 0.0 20-21 0.0 0.0 0.0 3.5959275793261624E-4 0.0 22-23 0.0 0.0 0.0 7.191855158652325E-4 0.0 24-25 0.0 0.0 0.0 0.0012422295274035833 0.0 26-27 0.0 0.0 0.0 0.0013076100288458771 0.0 28-29 0.0 0.0 0.0 0.001961415043268816 0.0 30-31 0.0 0.0 0.0 0.004609325351681718 0.0 32-33 0.0 0.0 0.0 0.008270633432450174 0.0 34-35 0.0 0.0 0.0 0.011245446248074545 0.0 36-37 0.0 0.0 0.0 0.012945339285574186 0.0 38-39 0.0 0.0 0.0 0.014351020066583502 0.0 40-41 0.0 0.0 0.0 0.016770098619948375 0.0 42-43 0.0 0.0 0.0 0.01981029193701504 0.0 44-45 0.0 0.0 0.0 0.02324276826273547 0.0 46-47 0.0 0.0 0.0 0.02696945684494622 0.0 48-49 0.0 0.0 0.0 0.03223258721105088 0.0 50-51 0.0 0.0 0.0 0.03981672537835696 0.0 52-53 0.0 0.0 0.0 0.05992122957186232 0.0 54-55 0.0 0.0 0.0 0.0819217683071942 0.0 56-57 0.0 0.0 0.0 0.10925081791007304 0.0 58-59 0.0 0.0 0.0 0.15008094106078557 0.0 60-61 0.0 0.0 0.0 0.20552360628385075 0.0 62-63 0.0 0.0 0.0 0.24599413667663067 0.0 64-65 0.0 0.0 0.0 0.28898181637493886 0.0 66-67 0.0 0.0 0.0 0.36858257688093166 0.0 68-69 0.0 0.0 0.0 0.4743682282145631 0.0 70-71 0.0 0.0 0.0 0.5598532338503623 0.0 72-73 0.0 0.0 0.0 0.6432787536907293 0.0 74-75 0.0 0.0 0.0 0.7825719120135364 0.0 76-77 0.0 0.0 0.0 0.9019893978978861 0.0 78-79 0.0 0.0 0.0 0.998752540032481 0.0 80-81 0.0 0.0 0.0 1.1151625228504853 0.0 82-83 0.0 0.0 0.0 1.2996336076699173 0.0 84-85 0.0 0.0 0.0 1.4965269877633853 0.0 86-87 0.0 0.0 0.0 1.732517907719345 0.0 88-89 0.0 0.0 0.0 2.025487934682264 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4060 0.0 47.434345 1 GTATCAA 7520 0.0 42.935436 1 ATCAACG 9440 0.0 34.089756 3 TCAACGC 9500 0.0 33.923378 4 CAACGCA 9625 0.0 33.430138 5 TATCAAC 9715 0.0 33.124786 2 AACGCAG 9930 0.0 32.484142 6 ACGCAGA 11370 0.0 28.244707 7 CGCAGAG 11390 0.0 28.19511 8 GTGTAGC 1045 0.0 27.757317 1 TAGCCCT 1535 0.0 26.011408 4 GCAGAGT 12290 0.0 25.819447 9 GTACATG 8800 0.0 24.316092 1 GAGTACT 6510 0.0 24.039513 12-13 AGTACTT 6725 0.0 23.83596 12-13 TAGGACG 560 0.0 23.766376 4 TACATGG 9060 0.0 23.45236 2 GTACTTT 7015 0.0 22.205927 14-15 ACATGGG 8820 0.0 22.204237 3 GTAGCCC 1015 0.0 21.542645 3 >>END_MODULE