FastQCFastQC Report
Thu 26 May 2016
SRR937660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529508
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103090.6740075893686074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86160.5633184004268039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57010.3727342387225173No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.13579530149564434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.049.1575851
GTATCAA81900.038.65011
ATCAACG105800.029.8156223
TCAACGC107250.029.412524
CAACGCA108200.029.1542785
TATCAAC108650.029.1209772
AACGCAG111000.028.4132826
ACGCAGA125850.025.0244757
CGCAGAG126400.024.7276888
GTACATG82050.024.6190131
TAGGACG6500.024.1191544
GGACGGT1201.7116395E-423.7490546
TACATGG83600.023.6968842
GTAGGAC10200.023.2879523
GCAGAGT134950.022.9498259
GAGTACT81000.022.90027612-13
ACATGGG84650.022.8417243
AGTACTT84000.022.44997412-13
GTACTTT87200.021.16171314-15
TAGCCCT14850.021.114414
Produced by FastQC (version 0.11.5)