FastQCFastQC Report
Thu 26 May 2016
SRR937659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670418
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.6528762652554078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.5032681103430994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.3436661903469179No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.13290215954822215No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8490.1266374112866898No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6960.10381582833396477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.033.0052221
GTATCAA48100.031.241021
TTACGCC350.00840713827.13286664-65
GTACATG42800.025.8878121
TACATGG42400.025.6795752
TATCAAC60300.024.9165552
ATCAACG59800.024.8068493
TCAACGC60050.024.5452164
CAACGCA60550.024.3425315
ACATGGG43250.024.2954163
GAGTACT34150.023.85266712-13
AGGACCG1401.7646335E-523.7732325
AACGCAG62450.023.6568226
CATGGGA28850.022.0839734
GTACTTT36950.022.04515814-15
GTGTAGC3900.021.9478031
GTATAGG1754.0057075E-621.7387771
TAGGGCA2657.8216544E-1021.5304744
ACGCAGA69550.021.2418177
AGTACTT36900.021.23836712-13
Produced by FastQC (version 0.11.5)