##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937659_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670418 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82645454030172 33.0 31.0 34.0 30.0 34.0 2 31.94821290597806 33.0 31.0 34.0 30.0 34.0 3 31.959836997216662 34.0 31.0 34.0 30.0 34.0 4 35.558942331500646 37.0 35.0 37.0 33.0 37.0 5 35.55680784227154 37.0 35.0 37.0 33.0 37.0 6 35.6391848070905 37.0 35.0 37.0 33.0 37.0 7 35.588362782622184 37.0 35.0 37.0 33.0 37.0 8 35.57987554033454 37.0 35.0 37.0 33.0 37.0 9 37.22161397814498 39.0 37.0 39.0 34.0 39.0 10-11 37.10268146141661 39.0 37.0 39.0 33.5 39.0 12-13 36.99049771933331 39.0 37.0 39.0 33.0 39.0 14-15 38.12713784534425 40.0 38.0 41.0 33.0 41.0 16-17 38.059744070117446 40.0 38.0 41.0 33.0 41.0 18-19 38.03340900751472 40.0 38.0 41.0 33.0 41.0 20-21 37.95754365187092 40.0 38.0 41.0 33.0 41.0 22-23 37.88096679981743 40.0 38.0 41.0 33.0 41.0 24-25 37.906259975119994 40.0 38.0 41.0 33.0 41.0 26-27 37.81927618291871 40.0 38.0 41.0 33.0 41.0 28-29 37.681757500544435 40.0 37.5 41.0 33.0 41.0 30-31 37.42604464677261 40.0 37.0 41.0 32.0 41.0 32-33 37.239734762491466 39.5 36.0 41.0 32.0 41.0 34-35 37.22005002848969 40.0 36.0 41.0 32.0 41.0 36-37 37.071103401161665 40.0 36.0 41.0 31.0 41.0 38-39 36.90603847152075 40.0 36.0 41.0 31.0 41.0 40-41 36.66540427017175 39.5 35.5 41.0 30.5 41.0 42-43 36.36898397716052 39.0 35.0 41.0 30.0 41.0 44-45 36.06715511815017 39.0 35.0 41.0 29.5 41.0 46-47 35.64628485512024 39.0 35.0 41.0 27.5 41.0 48-49 35.24726513906249 39.0 35.0 40.0 26.0 41.0 50-51 34.331690825723655 38.0 34.0 39.5 24.0 40.5 52-53 34.41305722698377 38.0 34.0 39.5 24.0 40.5 54-55 34.69054753899806 38.0 35.0 40.0 24.0 41.0 56-57 34.470384148396974 38.0 34.0 40.0 23.0 41.0 58-59 34.23080451300532 38.0 34.0 40.0 22.5 41.0 60-61 33.89960964651904 38.0 34.0 40.0 22.0 41.0 62-63 33.52855457341539 37.5 33.0 40.0 20.0 41.0 64-65 33.1541128072337 37.0 33.0 40.0 18.5 41.0 66-67 32.72540340503984 36.0 32.0 39.0 16.5 41.0 68-69 32.22693528515046 36.0 32.0 39.0 12.0 40.0 70-71 31.781042573439258 35.0 31.0 38.5 8.0 40.0 72-73 31.330343904847425 35.0 31.0 38.0 7.0 40.0 74-75 30.811080549746578 35.0 30.5 37.0 2.0 39.0 76-77 30.346676252725914 34.5 30.0 36.5 2.0 39.0 78-79 29.788778045935523 34.0 29.5 36.0 2.0 38.5 80-81 29.250862148689322 34.0 29.0 35.5 2.0 37.5 82-83 28.75619911756546 34.0 29.0 35.0 2.0 37.0 84-85 28.247729028755195 34.0 27.5 35.0 2.0 36.5 86-87 27.734631230068405 33.5 26.5 35.0 2.0 36.0 88-89 27.27471517769511 33.0 26.0 35.0 2.0 36.0 90-91 26.744737611460316 33.0 25.0 35.0 2.0 35.0 92-93 26.13657747852832 32.0 24.5 35.0 2.0 35.0 94-95 25.427842778684344 32.0 21.5 34.0 2.0 35.0 96-97 24.624846737408603 31.5 14.5 34.0 2.0 35.0 98-99 23.617516236139245 31.0 2.0 34.0 2.0 35.0 100-101 20.84867276833259 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10829099581696155 1101 2 0.3431764080695707 1101 3 0.15321035582802622 1101 4 -0.028495180181430158 1101 5 -0.07870106956501388 1101 6 0.1335669155509578 1101 7 0.12242214120595918 1101 8 0.1440331856305832 1101 9 0.11697123702106893 1101 10-11 0.29299935583585324 1101 12-13 0.1763878888022461 1101 14-15 0.24518634061077904 1101 16-17 0.4270036971187352 1101 18-19 0.4202133076354002 1101 20-21 0.3892447879078986 1101 22-23 0.30875634763685156 1101 24-25 0.3669423309728259 1101 26-27 0.43114253173554573 1101 28-29 0.3387185934004364 1101 30-31 0.4024731254616185 1101 32-33 0.03798887088410652 1101 34-35 0.4043617409464062 1101 36-37 0.4267450235877206 1101 38-39 0.4628014010044126 1101 40-41 0.4003539330569552 1101 42-43 0.42720476384896955 1101 44-45 0.36862482053100365 1101 46-47 0.6271204842208462 1101 48-49 0.398890008688376 1101 50-51 0.5579333611593853 1101 52-53 0.5947208014465986 1101 54-55 0.5129727092543419 1101 56-57 0.18147845387448314 1101 58-59 0.39003833326950854 1101 60-61 0.823621263528878 1101 62-63 0.572493151968466 1101 64-65 0.5862671015241858 1101 66-67 0.7168890663553924 1101 68-69 0.3977932361907577 1101 70-71 0.6218873391062125 1101 72-73 0.6219964564963689 1101 74-75 0.7659776073167421 1101 76-77 0.6495382937836851 1101 78-79 0.8389434576337678 1101 80-81 0.7859320815839332 1101 82-83 0.8016580392842627 1101 84-85 0.837458005526333 1101 86-87 1.1039829947004485 1101 88-89 1.3080340441866731 1101 90-91 0.9651671705542952 1101 92-93 0.8752521153199275 1101 94-95 1.0848833294461109 1101 96-97 1.4789355047402317 1101 98-99 1.6374491832966598 1101 100-101 1.920359524789248 1102 1 0.38620835731409286 1102 2 0.517771553520749 1102 3 0.4605866760058994 1102 4 0.3384012437577937 1102 5 0.28279976423154096 1102 6 0.21687245566583613 1102 7 0.22033209117816455 1102 8 0.41455940389999313 1102 9 0.3616627322962245 1102 10-11 0.3697188296175895 1102 12-13 0.5012183501696725 1102 14-15 0.7179276783910282 1102 16-17 0.7141419220585234 1102 18-19 0.7981139932014543 1102 20-21 0.5820556087342794 1102 22-23 0.6970378002957318 1102 24-25 0.6819534482602094 1102 26-27 0.44398577798346395 1102 28-29 0.7294124974211869 1102 30-31 0.6916671221264252 1102 32-33 0.7073487004190326 1102 34-35 0.8081212369627124 1102 36-37 0.9520173971285786 1102 38-39 0.9895282863110708 1102 40-41 1.1060163380967865 1102 42-43 1.0043541060761356 1102 44-45 1.168844386956792 1102 46-47 0.959715444383896 1102 48-49 1.1209448539727944 1102 50-51 1.2709702334958664 1102 52-53 1.3751015685394279 1102 54-55 1.1654787311183412 1102 56-57 1.1089038754049554 1102 58-59 0.9525693690301225 1102 60-61 0.9469823900806631 1102 62-63 1.0288939301785902 1102 64-65 1.1196550948538118 1102 66-67 0.945613357628325 1102 68-69 0.9665555115289095 1102 70-71 1.162853616667821 1102 72-73 1.2903158932204732 1102 74-75 1.268516054602273 1102 76-77 1.2695772042895221 1102 78-79 1.2985960424030871 1102 80-81 1.1990124966293294 1102 82-83 1.349713440433046 1102 84-85 1.3069975645406018 1102 86-87 1.3142738970536065 1102 88-89 1.2146201105198209 1102 90-91 1.0479807707025266 1102 92-93 1.4231175961426068 1102 94-95 1.7252224067112572 1102 96-97 2.1870594624945383 1102 98-99 1.9924769765151176 1102 100-101 2.2169835751876157 1103 1 0.11311406265837576 1103 2 -0.03964141630788376 1103 3 -0.00682896265166022 1103 4 -0.16996307008968614 1103 5 -0.19196450337890525 1103 6 -0.12934265194576966 1103 7 -0.10407851410203506 1103 8 -0.03372566102734709 1103 9 0.027062980140335924 1103 10-11 0.04377917234962325 1103 12-13 0.031813838998836275 1103 14-15 -0.10092768298030563 1103 16-17 0.07590933276749467 1103 18-19 0.049793910518758366 1103 20-21 0.034592101145122456 1103 22-23 -0.024343259178358778 1103 24-25 0.03153604001607846 1103 26-27 0.18263008088364785 1103 28-29 -0.10502321524963776 1103 30-31 0.0985674898128579 1103 32-33 -0.036368927281031915 1103 34-35 0.1010983071325171 1103 36-37 0.11312117037670788 1103 38-39 0.23587479549983215 1103 40-41 0.4099738544199951 1103 42-43 0.3140003077546041 1103 44-45 0.014778162601771783 1103 46-47 0.06904053664548115 1103 48-49 0.21470914368182292 1103 50-51 0.39481409510433707 1103 52-53 0.49443901900885123 1103 54-55 0.5325336528978539 1103 56-57 0.47752694666609585 1103 58-59 0.591113038380918 1103 60-61 0.4233853526507616 1103 62-63 0.47472775223059216 1103 64-65 0.5376498013931226 1103 66-67 0.30807517382591243 1103 68-69 0.4255651890085872 1103 70-71 0.5032831451350539 1103 72-73 0.3358627736654398 1103 74-75 0.17987013680559727 1103 76-77 0.2480310853957448 1103 78-79 0.04779666864294185 1103 80-81 0.2685074420033331 1103 82-83 0.5153474232947453 1103 84-85 0.3509507971383883 1103 86-87 0.13184669063753418 1103 88-89 0.5501769013295323 1103 90-91 0.8716612727823438 1103 92-93 0.8393019187275321 1103 94-95 0.7125242206649816 1103 96-97 0.7644597511360374 1103 98-99 0.9529341112127803 1103 100-101 1.42497289307234 1104 1 0.0309809654218256 1104 2 0.11454418922459197 1104 3 0.11349151084322173 1104 4 0.17737108121674794 1104 5 0.1286013620459272 1104 6 0.08953196114366335 1104 7 -0.05975111107367326 1104 8 0.13517300325976578 1104 9 0.26214600905754537 1104 10-11 0.26206470537993454 1104 12-13 0.12506190096030423 1104 14-15 0.08952904577795096 1104 16-17 0.14076129590293363 1104 18-19 0.1890886875746105 1104 20-21 0.33761104930607644 1104 22-23 0.2547403901900438 1104 24-25 0.11644461060809164 1104 26-27 0.09817900589968076 1104 28-29 0.1335324231876669 1104 30-31 0.13022008594793988 1104 32-33 0.24181105933395486 1104 34-35 0.04761402058166908 1104 36-37 0.3081545980558005 1104 38-39 0.29875124902873296 1104 40-41 0.4052969421754966 1104 42-43 0.7211827273748099 1104 44-45 0.47062330077415737 1104 46-47 0.5620900890840659 1104 48-49 0.3716597655272835 1104 50-51 0.43276618790710586 1104 52-53 0.4673613789541946 1104 54-55 0.45947726852485715 1104 56-57 0.6449624964276737 1104 58-59 0.7343126493789285 1104 60-61 0.7407679200638313 1104 62-63 0.6265091094152737 1104 64-65 0.9229578310074658 1104 66-67 0.9509506165073702 1104 68-69 0.5588532665858921 1104 70-71 0.6179245731487732 1104 72-73 0.6856438729097434 1104 74-75 0.6326949325447018 1104 76-77 0.5871712725070886 1104 78-79 0.528426740308543 1104 80-81 0.6653469752009542 1104 82-83 0.5929308356368459 1104 84-85 0.8536548140369717 1104 86-87 0.720582538773396 1104 88-89 0.5533113998097754 1104 90-91 0.5632560763293704 1104 92-93 0.24217080832904614 1104 94-95 0.4901682838534036 1104 96-97 0.8010175716095311 1104 98-99 0.8582926483422533 1104 100-101 1.1772098287406152 1105 1 0.2670094277807742 1105 2 0.29745835303753765 1105 3 0.17359859555662993 1105 4 0.01724926007488392 1105 5 0.1706317611926167 1105 6 0.14050246021736967 1105 7 0.20650808165073897 1105 8 0.08465512305991041 1105 9 0.2727948329502041 1105 10-11 0.14371364155014277 1105 12-13 0.2515443154890207 1105 14-15 0.3022406187864348 1105 16-17 0.5588600861123325 1105 18-19 0.6280798737869304 1105 20-21 0.4250156133884886 1105 22-23 0.4166323370827598 1105 24-25 0.46452431363396585 1105 26-27 0.3627049139443699 1105 28-29 0.25105368574871534 1105 30-31 0.4707807017902965 1105 32-33 0.6041272048456605 1105 34-35 0.5549120613384204 1105 36-37 0.5171539945639765 1105 38-39 0.6198896030398515 1105 40-41 0.5863848414625323 1105 42-43 0.6745191249496116 1105 44-45 0.7827654160832012 1105 46-47 0.7798664322042583 1105 48-49 0.6587166200717149 1105 50-51 0.6968289510576113 1105 52-53 0.7950732326565486 1105 54-55 0.8813952497745063 1105 56-57 1.058668427489259 1105 58-59 0.9234985669672113 1105 60-61 1.1667121040867343 1105 62-63 1.0041949989341674 1105 64-65 1.1765629929678312 1105 66-67 1.1517081391032349 1105 68-69 0.861660556205841 1105 70-71 1.117705281058722 1105 72-73 0.9574958910987021 1105 74-75 1.1591362955941626 1105 76-77 0.9271108531504417 1105 78-79 0.9070757606869186 1105 80-81 1.1560244298688929 1105 82-83 1.2924778658956448 1105 84-85 1.064331356449813 1105 86-87 1.112015410833127 1105 88-89 1.2046246962786462 1105 90-91 1.3420254442067652 1105 92-93 1.3482713388404726 1105 94-95 1.3136206079986898 1105 96-97 1.4417153765834279 1105 98-99 1.7236402877067611 1105 100-101 1.606420587736853 1106 1 0.2375217650477346 1106 2 0.26953433585607556 1106 3 0.07018052851248413 1106 4 0.15979367382171006 1106 5 0.2170917090849116 1106 6 0.2239907774033938 1106 7 0.29482402503641936 1106 8 0.14031262048144555 1106 9 0.279608599658431 1106 10-11 0.07738711093789874 1106 12-13 0.043436554422349616 1106 14-15 -0.05138351810193598 1106 16-17 0.14974310057242946 1106 18-19 0.28854925739992154 1106 20-21 0.4820234378294117 1106 22-23 0.47383484057248637 1106 24-25 0.14153416770752614 1106 26-27 0.24686466674339158 1106 28-29 0.11591639559823363 1106 30-31 0.25236323535173 1106 32-33 0.24557913776008888 1106 34-35 0.2817400768961775 1106 36-37 0.034743453728999896 1106 38-39 0.19222840257427265 1106 40-41 0.34016555001142734 1106 42-43 0.3673539004266857 1106 44-45 0.13390739509929261 1106 46-47 0.10720682645004587 1106 48-49 0.15011449848429947 1106 50-51 0.14264293729752353 1106 52-53 0.04293901180766113 1106 54-55 -0.06854220440342829 1106 56-57 0.043511420907442755 1106 58-59 0.10523456560859756 1106 60-61 -0.18127669565479465 1106 62-63 0.009063010681181538 1106 64-65 -0.0038506378792675378 1106 66-67 0.006410259448017541 1106 68-69 -0.289318223777844 1106 70-71 -0.24075787282470884 1106 72-73 0.20177667627658735 1106 74-75 0.25451763871391364 1106 76-77 0.21445195155448715 1106 78-79 0.04874722529467945 1106 80-81 0.058765676560383895 1106 82-83 0.09515097492005609 1106 84-85 0.33307025042429217 1106 86-87 0.48191078119966235 1106 88-89 0.6216054727581017 1106 90-91 0.4931812992827105 1106 92-93 0.6121908908301315 1106 94-95 0.5285411002467377 1106 96-97 0.49172200395530297 1106 98-99 0.8890739870643216 1106 100-101 0.6589466519478044 1107 1 0.2422564772060234 1107 2 0.2743741622804272 1107 3 0.35330808924453194 1107 4 0.23758508598617567 1107 5 0.2754141311004048 1107 6 0.20056629172064078 1107 7 0.2042977910292052 1107 8 0.24361937818621016 1107 9 0.32433243477527895 1107 10-11 0.4395651699344185 1107 12-13 0.479188886930757 1107 14-15 0.3532846978576103 1107 16-17 0.5544615885722664 1107 18-19 0.617013058103268 1107 20-21 0.4721518371904949 1107 22-23 0.24821049610846302 1107 24-25 0.11307562271747429 1107 26-27 0.2953669027066468 1107 28-29 0.4083328583203709 1107 30-31 0.23843902273755901 1107 32-33 0.21711030985270696 1107 34-35 0.34150667752366104 1107 36-37 0.3714975708948529 1107 38-39 0.2444119229424473 1107 40-41 0.10551508922247876 1107 42-43 0.24377265738193188 1107 44-45 0.45152043292442556 1107 46-47 0.3772670843456112 1107 48-49 0.3075425352011649 1107 50-51 -0.20766863509763311 1107 52-53 -0.12770485877382498 1107 54-55 -0.12286114393759107 1107 56-57 0.03208252956589064 1107 58-59 0.1049603544797435 1107 60-61 0.09793193102731834 1107 62-63 0.22947277351141082 1107 64-65 0.018875751971258126 1107 66-67 -0.07751205653917737 1107 68-69 0.1774085408278978 1107 70-71 0.2503371207656073 1107 72-73 0.32655873555646764 1107 74-75 0.4174436085644011 1107 76-77 0.37566638694234555 1107 78-79 0.25706884752771586 1107 80-81 0.2299568268532184 1107 82-83 0.41663724494032195 1107 84-85 0.2864463606937271 1107 86-87 0.13812314857858965 1107 88-89 0.07897706768428137 1107 90-91 0.252371371011769 1107 92-93 0.08870813528249855 1107 94-95 0.33332791251951477 1107 96-97 0.2770775301093309 1107 98-99 0.4840378711735589 1107 100-101 0.5562214503454967 1108 1 -0.0836865274162335 1108 2 0.06662356605530917 1108 3 0.09330328861384984 1108 4 0.13548580101588925 1108 5 0.1267927709363903 1108 6 0.13369658920559147 1108 7 0.19919326720552988 1108 8 -0.00687885240702002 1108 9 0.286973398248648 1108 10-11 0.33319926938675337 1108 12-13 0.19843457131791808 1108 14-15 0.320512685974947 1108 16-17 0.20717119225598424 1108 18-19 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-1.0116543371544253 2316 74-75 -1.0522763609372277 2316 76-77 -1.31633472208933 2316 78-79 -1.0792311455408345 2316 80-81 -0.9314488707970199 2316 82-83 -0.8793737067474829 2316 84-85 -1.2832562801879526 2316 86-87 -1.10220748149003 2316 88-89 -1.1577596066069766 2316 90-91 -1.2839598135726895 2316 92-93 -1.3672875672197513 2316 94-95 -1.0255928610300806 2316 96-97 -1.0400327492280255 2316 98-99 -1.5853285944811155 2316 100-101 -1.0602753958456752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2529.0 3 483.0 4 402.0 5 459.0 6 517.0 7 686.0 8 834.0 9 1059.0 10 1327.0 11 1711.0 12 2520.0 13 3333.0 14 3874.0 15 5117.0 16 6794.0 17 9031.0 18 7708.0 19 4973.0 20 4653.0 21 4636.0 22 5096.0 23 5461.0 24 6378.0 25 6953.0 26 8114.0 27 9519.0 28 11336.0 29 13572.0 30 16744.0 31 20925.0 32 26275.0 33 34559.0 34 47355.0 35 66354.0 36 95426.0 37 129140.0 38 94451.0 39 10110.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67816487412305 19.17712524868738 14.61055182420607 21.5341580529835 2 14.941304900480029 20.355909138490528 41.8259036054493 22.876882355580147 3 19.531927620756694 25.973082099596233 29.68805144309855 24.80693883654853 4 12.109479565299967 16.552488369067326 37.811485424508476 33.52654664112423 5 12.77107424667532 38.67066685012473 33.83874036097369 14.719518542226265 6 30.686751399131758 36.742656893292384 17.807865025815754 14.762726681760105 7 27.851118390378616 34.10313579686062 20.29630470907504 17.749441103685726 8 26.282015837743085 35.41748547808375 19.387113116275458 18.913385567897713 9 27.005968516597978 17.11876361745305 20.590784514733002 35.28448335121597 10-11 24.764957041425248 27.59533605426724 26.65979708069995 20.979909823607564 12-13 26.039190136267386 25.035826413368817 27.810038568497564 21.114944881866233 14-15 22.7486009309693 26.042334446611225 27.08657416746987 24.122490454949606 16-17 21.046056087448058 29.743563303676375 27.46440828422263 21.745972324652936 18-19 21.040704372892044 27.59055336966576 30.925923988816344 20.44281826862585 20-21 22.17737808217336 26.49636187178876 30.911201878572665 20.41505816746522 22-23 21.49398159251149 26.711702928029396 30.87972374571995 20.91459173373917 24-25 21.16246126075509 26.683158552220654 31.307137584464257 20.847242602560005 26-27 20.969592827792304 27.00463205080314 31.155920806873365 20.869854314531192 28-29 20.760841301105575 27.27354241325614 31.2372039919062 20.728412293732084 30-31 21.000413980897097 27.068820961481844 31.0779345192815 20.85283053833956 32-33 20.70799158277338 27.165657356703893 30.965365623281283 21.160985437241443 34-35 21.326645902952553 27.157847069865316 30.840691300163197 20.674815727018935 36-37 21.30308981985825 26.9170457440363 30.896330899618025 20.883533536487427 38-39 21.014230209818837 27.250526844216218 30.86033417723032 20.874908768734628 40-41 21.19307760981058 27.288008337348856 30.805234002338345 20.71368005050222 42-43 21.01808607528356 27.305289095531865 30.419079625203505 21.257545203981074 44-45 21.017978478426873 27.59519358507088 30.278067929986896 21.108760006515347 46-47 21.358069890986798 27.80180368657397 29.740804112465163 21.099322309974074 48-49 21.387720761190863 27.950107746704088 29.323168380750847 21.339003111354202 50-51 21.5126236284675 28.33703459056523 29.117661124245675 21.032680656721592 52-53 21.582719295426536 28.37800569655777 28.948633072770498 21.09064193524519 54-55 21.164751289909496 28.38704905466407 29.133425877086154 21.314773778340278 56-57 21.412937270850204 28.666919658998207 28.757608445561328 21.162534624590258 58-59 21.531140866901975 28.23971464321064 28.889013390105887 21.3401310997815 60-61 21.354622622060347 28.347415974734908 29.219264933924126 21.07869646928062 62-63 21.38766667911702 28.248608672916742 29.33746685093191 21.026257797034326 64-65 21.226501772591387 28.24597861536186 29.241707364807223 21.285812247239523 66-67 21.238922187894037 28.42680128362187 28.976434064981866 21.357842463502234 68-69 21.291536649666405 28.402361559442017 29.0584914018827 21.247610389008873 70-71 21.2971512065689 28.40994657391803 28.838804427073995 21.454097792439075 72-73 21.241479766195447 28.494201572391642 28.741675848288484 21.522642813124428 74-75 21.108543861497008 28.837260030015155 28.634519522177825 21.41967658631001 76-77 21.3434394596121 28.500351861729545 28.683454430544288 21.472754248114065 78-79 21.28638867327721 28.53099093136028 28.61854712009616 21.56407327526635 80-81 21.237277436849396 28.84687356433462 28.483900004855585 21.4319489939604 82-83 21.358007077030873 28.777460470890727 28.485011918305638 21.379520533772766 84-85 21.325567046963723 28.7821431185753 28.293189759616606 21.599100074844372 86-87 21.334702718852704 28.671347475351062 28.330445174783385 21.66350463101285 88-89 21.423705416767508 28.503240379702582 28.271902068524547 21.801152135005363 90-91 21.199548495970156 28.57757554886052 28.46970463239848 21.753171322770847 92-93 21.40363378867735 28.42815185304698 28.496946446284944 21.67126791199073 94-95 21.20954067035684 28.505927436523766 28.42301055509491 21.861521338024488 96-97 21.154469601332625 28.343629314788117 28.455602781782463 22.046298302096794 98-99 21.158489032578068 28.434546557385865 28.151359685424282 22.255604724611786 100-101 21.576759456762684 27.96914863742554 28.578737146510363 21.875354759301416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 980.0 1 579.5 2 246.0 3 314.5 4 264.0 5 253.5 6 412.5 7 774.0 8 1253.0 9 1468.0 10 1335.0 11 1199.0 12 1204.5 13 1253.0 14 1318.0 15 1381.5 16 1467.0 17 1571.0 18 1644.0 19 1730.0 20 1893.0 21 2155.5 22 2544.0 23 3026.5 24 3597.0 25 4379.5 26 5313.0 27 6472.0 28 7464.5 29 8345.0 30 9640.5 31 11067.0 32 12517.0 33 13752.5 34 14953.5 35 16325.5 36 17964.0 37 19398.0 38 20184.5 39 20783.5 40 21176.5 41 21866.5 42 22464.0 43 23231.0 44 24208.5 45 24870.5 46 24969.5 47 25060.5 48 26827.0 49 26102.5 50 23500.5 51 21821.0 52 19992.0 53 18918.5 54 18040.0 55 16849.0 56 15276.0 57 13364.0 58 12040.5 59 10671.0 60 8717.0 61 7131.5 62 5776.0 63 4273.5 64 2931.0 65 2179.5 66 1632.5 67 1198.5 68 894.5 69 632.5 70 449.0 71 341.0 72 255.0 73 206.0 74 164.0 75 126.0 76 94.5 77 66.0 78 47.0 79 36.0 80 20.5 81 15.5 82 13.5 83 8.5 84 8.0 85 4.0 86 2.5 87 2.5 88 1.5 89 2.0 90 1.5 91 1.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2845985638810414 2 0.25476642930231586 3 0.2562580360312522 4 0.2576004820872948 5 0.2658043190964443 6 0.18689832313571533 7 0.18525755573388542 8 0.18749496582728983 9 0.1849592343880982 10-11 0.19167146466831142 12-13 0.181826860257332 14-15 0.1824235029489065 16-17 0.1889119922197793 18-19 0.183020145640481 20-21 0.1769791383882891 22-23 0.1759350136780337 24-25 0.17899280747235305 26-27 0.174219665939757 28-29 0.18853909053754525 30-31 0.19465467812618398 32-33 0.19599712418222662 34-35 0.1942071961075031 36-37 0.1699685867622886 38-39 0.16444964186522437 40-41 0.17011774743518224 42-43 0.17921654848169352 44-45 0.18331846698626827 46-47 0.18391510967784278 48-49 0.18734580515439622 50-51 0.19032901861226875 52-53 0.21143525382671707 54-55 0.17675539737894866 56-57 0.16348009749141582 58-59 0.16116810706156456 60-61 0.15453045711779817 62-63 0.16303261547273493 64-65 0.1579611525943516 66-67 0.15840863461303248 68-69 0.16623956993994793 70-71 0.16139184807090504 72-73 0.15743909023922387 74-75 0.16251055311760723 76-77 0.16720911431375649 78-79 0.16907362272492685 80-81 0.1619139104260327 82-83 0.15952733965973467 84-85 0.15348633240754275 86-87 0.15184556500571286 88-89 0.1569170278840962 90-91 0.16400215984654348 92-93 0.1542321357720109 94-95 0.16004940201486237 96-97 0.1585577952859261 98-99 0.15990024134196873 100-101 0.1417026392489462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 670418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.65056297080595 #Duplication Level Percentage of deduplicated Percentage of total 1 84.31828090450377 58.728157337292444 2 10.006855299963249 13.939662104196671 3 2.6670620676835655 5.5728712347672635 4 1.021377933091439 2.845581921831076 5 0.5359906031282511 1.8666023627472255 6 0.327042045859205 1.3667197565530658 7 0.20527971395570854 1.0008493350450778 8 0.15063218646274829 0.8393293270923067 9 0.12829674579525083 0.804234651476544 >10 0.5864583776370579 7.2163757277513145 >50 0.033006300484180635 1.577136212415715 >100 0.01757457997575933 2.052572146445155 >500 0.0015002690218803012 0.688917851207417 >1k 6.429724379487006E-4 1.5009900311788902 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4377 0.6528762652554078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3374 0.5032681103430994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2304 0.3436661903469179 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 891 0.13290215954822215 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 849 0.1266374112866898 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 696 0.10381582833396477 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.458033644681378E-5 0.0 16-17 0.0 0.0 0.0 2.983213457872551E-4 0.0 18-19 7.458033644681378E-5 0.0 0.0 2.983213457872551E-4 0.0 20-21 1.4916067289362755E-4 0.0 0.0 2.983213457872551E-4 0.0 22-23 2.983213457872551E-4 0.0 0.0 4.4748201868088266E-4 7.458033644681378E-5 24-25 2.983213457872551E-4 0.0 0.0 5.220623551276965E-4 2.983213457872551E-4 26-27 2.983213457872551E-4 0.0 0.0 8.203837009149516E-4 2.983213457872551E-4 28-29 2.983213457872551E-4 0.0 0.0 0.0015661870653830893 2.983213457872551E-4 30-31 2.983213457872551E-4 0.0 0.0 0.0037290168223406885 2.983213457872551E-4 32-33 2.983213457872551E-4 0.0 0.0 0.00611558758863873 2.983213457872551E-4 34-35 2.983213457872551E-4 0.0 0.0 0.007980095999809075 4.4748201868088266E-4 36-37 2.983213457872551E-4 0.0 0.0 0.010590407775447556 4.4748201868088266E-4 38-39 2.983213457872551E-4 0.0 0.0 0.014468585270681872 4.4748201868088266E-4 40-41 2.983213457872551E-4 0.0 0.0 0.018794244784597073 4.4748201868088266E-4 42-43 3.7290168223406885E-4 0.0 0.0 0.027072662130193402 4.4748201868088266E-4 44-45 5.966426915745102E-4 0.0 0.0 0.040870024372853954 5.220623551276965E-4 46-47 8.949640373617653E-4 0.0 0.0 0.05436906526972725 5.966426915745102E-4 48-49 8.949640373617653E-4 0.0 0.0 0.08464868186713365 5.966426915745102E-4 50-51 8.949640373617653E-4 0.0 0.0 0.11589784283834861 5.966426915745102E-4 52-53 8.949640373617653E-4 0.0 0.0 0.1444621116974783 5.966426915745102E-4 54-55 8.949640373617653E-4 0.0 0.0 0.18488465405165133 5.966426915745102E-4 56-57 8.949640373617653E-4 0.0 0.0 0.23075156096644184 5.966426915745102E-4 58-59 8.949640373617653E-4 0.0 0.0 0.28623933128287127 5.966426915745102E-4 60-61 8.949640373617653E-4 0.0 0.0 0.3563448475428762 7.458033644681378E-4 62-63 8.949640373617653E-4 0.0 0.0 0.4591165511665857 7.458033644681378E-4 64-65 8.949640373617653E-4 0.0 0.0 0.5433923313514852 7.458033644681378E-4 66-67 8.949640373617653E-4 0.0 0.0 0.6608563612552169 7.458033644681378E-4 68-69 0.001044124710255393 0.0 0.0 0.8027827415135035 7.458033644681378E-4 70-71 0.001044124710255393 0.0 0.0 0.916368593922001 7.458033644681378E-4 72-73 0.001044124710255393 0.0 0.0 1.0670954538810116 7.458033644681378E-4 74-75 0.0011187050467022066 0.0 0.0 1.2541429376896205 7.458033644681378E-4 76-77 0.0011932853831490204 0.0 0.0 1.4154602054240786 7.458033644681378E-4 78-79 0.0011932853831490204 0.0 0.0 1.5811777130088989 7.458033644681378E-4 80-81 0.0017899280747235306 0.0 0.0 1.8144650054145324 7.458033644681378E-4 82-83 0.0017899280747235306 0.0 0.0 2.0760182453335085 7.458033644681378E-4 84-85 0.0018645084111703445 0.0 0.0 2.3367511015515694 7.458033644681378E-4 86-87 0.0019390887476171583 0.0 0.0 2.674450864982742 7.458033644681378E-4 88-89 0.0019390887476171583 0.0 0.0 3.055929285908195 7.458033644681378E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3285 0.0 33.005222 1 GTATCAA 4810 0.0 31.24102 1 TTACGCC 35 0.008407138 27.132866 64-65 GTACATG 4280 0.0 25.887812 1 TACATGG 4240 0.0 25.679575 2 TATCAAC 6030 0.0 24.916555 2 ATCAACG 5980 0.0 24.806849 3 TCAACGC 6005 0.0 24.545216 4 CAACGCA 6055 0.0 24.342531 5 ACATGGG 4325 0.0 24.295416 3 GAGTACT 3415 0.0 23.852667 12-13 AGGACCG 140 1.7646335E-5 23.773232 5 AACGCAG 6245 0.0 23.656822 6 CATGGGA 2885 0.0 22.083973 4 GTACTTT 3695 0.0 22.045158 14-15 GTGTAGC 390 0.0 21.947803 1 GTATAGG 175 4.0057075E-6 21.738777 1 TAGGGCA 265 7.8216544E-10 21.530474 4 ACGCAGA 6955 0.0 21.241817 7 AGTACTT 3690 0.0 21.238367 12-13 >>END_MODULE