FastQCFastQC Report
Thu 26 May 2016
SRR937659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670418
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.8937707519786163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.7353621173655839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.5213165517632283No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.18481007371520455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.1402110325200099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.039.4846421
CGTGCGA250.001615320438.0028310-11
GTATCAA55300.035.3160631
AGGACCG1353.074092E-1035.1983155
GCCGGTC600.00394834531.6690278
ACCGAGT1551.3533281E-930.6474488
GGACCGA1401.7049388E-830.5334386
TATCAAC70650.027.6430052
ATCAACG71200.027.429473
TCAACGC71550.027.2288824
CAACGCA72200.027.1153765
AACGCAG74050.026.4261176
ACGCAGA81650.023.9117667
GAGTACT48950.023.6789912-13
CGCAGAG81950.023.650338
AGTACTT50950.022.88934112-13
GTACTTT51550.022.34314314-15
GCAGAGT86400.022.3222689
GTACATG41550.022.3007351
TACATGG41750.022.1939052
Produced by FastQC (version 0.11.5)