##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937659_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670418 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16339955072805 33.0 31.0 34.0 31.0 34.0 2 32.443781342386394 34.0 31.0 34.0 31.0 34.0 3 32.52785426405615 34.0 31.0 34.0 31.0 34.0 4 36.1597421310287 37.0 35.0 37.0 35.0 37.0 5 36.08985289774439 37.0 35.0 37.0 35.0 37.0 6 36.14192638025828 37.0 35.0 37.0 35.0 37.0 7 36.08828223585882 37.0 35.0 37.0 35.0 37.0 8 36.06938208699647 37.0 35.0 37.0 35.0 37.0 9 37.758046472499245 39.0 37.0 39.0 35.0 39.0 10-11 37.68690876438282 39.0 37.0 39.0 35.0 39.0 12-13 37.57842420698728 39.0 37.0 39.0 35.0 39.0 14-15 38.76465801932525 40.0 38.0 41.0 35.0 41.0 16-17 38.729702961436004 40.0 38.0 41.0 34.5 41.0 18-19 38.76035324230555 40.0 38.0 41.0 35.0 41.0 20-21 38.69228004021372 40.0 38.0 41.0 34.5 41.0 22-23 38.641683248361474 40.0 38.0 41.0 34.0 41.0 24-25 38.65996736364477 40.0 38.0 41.0 34.0 41.0 26-27 38.512617501320065 40.0 38.0 41.0 34.0 41.0 28-29 38.34536811362463 40.0 38.0 41.0 34.0 41.0 30-31 38.32309842516162 40.0 38.0 41.0 34.0 41.0 32-33 38.17598125348663 40.0 37.0 41.0 34.0 41.0 34-35 38.004874570790165 40.0 37.0 41.0 33.5 41.0 36-37 37.82803638923776 40.0 37.0 41.0 33.0 41.0 38-39 37.666071764182945 40.0 37.0 41.0 33.0 41.0 40-41 37.445516081012144 40.0 36.5 41.0 33.0 41.0 42-43 37.17135428941347 40.0 36.0 41.0 32.5 41.0 44-45 36.77621424245769 40.0 36.0 41.0 31.0 41.0 46-47 36.2554048369823 39.5 36.0 41.0 30.0 41.0 48-49 35.802849863816306 39.0 35.0 41.0 28.5 41.0 50-51 35.486327186919205 39.0 35.0 41.0 27.0 41.0 52-53 35.23851910300738 39.0 35.0 40.0 26.5 41.0 54-55 35.00441888493447 39.0 35.0 40.0 26.0 41.0 56-57 34.73981680086155 38.0 35.0 40.0 25.5 41.0 58-59 34.483689280419085 38.0 34.0 40.0 25.0 41.0 60-61 34.149876644123516 38.0 34.0 40.0 23.5 41.0 62-63 33.78087402187889 37.5 34.0 40.0 22.5 41.0 64-65 33.36966787884573 37.0 33.0 39.5 21.5 41.0 66-67 32.95875707394491 36.0 33.0 39.0 20.0 40.5 68-69 32.516981196805574 36.0 32.0 39.0 18.5 40.0 70-71 32.1229934160479 35.0 32.0 38.5 17.5 40.0 72-73 31.645394365903066 35.0 31.0 37.5 13.5 40.0 74-75 31.180378510123536 35.0 31.0 37.0 9.5 39.0 76-77 29.961031028403177 33.5 29.5 35.5 8.0 38.5 78-79 30.533330698161446 34.0 31.0 36.0 7.0 38.5 80-81 30.34317396012637 34.0 31.0 36.0 4.0 37.5 82-83 29.9667670020793 34.0 30.0 35.0 2.0 37.0 84-85 29.58241500079055 34.0 30.0 35.0 2.0 36.5 86-87 29.21766345772339 34.0 29.5 35.0 2.0 36.0 88-89 28.76584160926467 34.0 29.0 35.0 2.0 36.0 90-91 28.211234632721677 34.0 29.0 35.0 2.0 35.0 92-93 27.781868177763723 33.0 27.5 35.0 2.0 35.0 94-95 27.314812549782374 33.0 26.5 35.0 2.0 35.0 96-97 26.737985853601785 33.0 25.5 35.0 2.0 35.0 98-99 25.84738849493898 32.5 24.5 34.0 2.0 35.0 100-101 23.098063446983822 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3333633160316509 1101 2 0.24590828278575572 1101 3 0.25169551313980065 1101 4 0.05810879111621148 1101 5 -0.04327688185109935 1101 6 -0.007832506101202341 1101 7 0.06587735287444474 1101 8 0.16290678000226677 1101 9 0.19966216811598514 1101 10-11 0.36559083588455366 1101 12-13 0.32134490913635005 1101 14-15 0.3221143976214975 1101 16-17 0.33769383583929624 1101 18-19 0.33196861138620903 1101 20-21 0.25967678901882607 1101 22-23 0.15476693256614027 1101 24-25 0.14908774965690696 1101 26-27 0.1994310554885672 1101 28-29 0.16836480293895306 1101 30-31 0.3006959514860057 1101 32-33 0.5720469324825217 1101 34-35 0.5995589314319218 1101 36-37 0.5647980545707725 1101 38-39 0.609454336260093 1101 40-41 0.8098412429561606 1101 42-43 0.8813872797739393 1101 44-45 0.8625499968802828 1101 46-47 0.8266490020071515 1101 48-49 0.5516470870670673 1101 50-51 0.7645190167437192 1101 52-53 0.7141523828656204 1101 54-55 0.795000410110859 1101 56-57 1.0587683691827934 1101 58-59 0.8773741858175015 1101 60-61 1.0850353419763863 1101 62-63 0.6086681367919127 1101 64-65 0.7979826718175929 1101 66-67 0.6423919393840265 1101 68-69 0.8987019481084673 1101 70-71 0.9423154594293912 1101 72-73 0.8826972007417275 1101 74-75 1.0387842948402835 1101 76-77 0.9348112027945028 1101 78-79 1.0191367193708025 1101 80-81 0.9390863506180231 1101 82-83 1.125034994409905 1101 84-85 1.0842908399018718 1101 86-87 1.0526450124989104 1101 88-89 0.8432900090917919 1101 90-91 1.151562260404809 1101 92-93 0.6004787800571485 1101 94-95 0.5060503275894348 1101 96-97 0.6648442179344123 1101 98-99 0.24793205372881744 1101 100-101 -0.05570133357387519 1102 1 0.376963464262154 1102 2 0.21924717476277777 1102 3 0.23152134230414845 1102 4 0.053958337160310066 1102 5 0.17227249743368844 1102 6 0.010948298049257232 1102 7 0.17375765674697163 1102 8 0.1719525461686615 1102 9 0.2726323367281793 1102 10-11 0.20920673360551234 1102 12-13 0.32967916891030313 1102 14-15 0.3687236249700234 1102 16-17 0.29978944451078604 1102 18-19 0.3039901048084843 1102 20-21 0.3805235557410782 1102 22-23 0.25286587642383296 1102 24-25 0.3020375365755612 1102 26-27 0.3697386337727977 1102 28-29 0.3860477749604385 1102 30-31 0.38772948863627477 1102 32-33 0.539992087198101 1102 34-35 0.5227255054545239 1102 36-37 0.3943033352822809 1102 38-39 0.42181398050690433 1102 40-41 0.2519961438270215 1102 42-43 0.24149752620714793 1102 44-45 0.3088284849292009 1102 46-47 0.34721227790116416 1102 48-49 0.46492020176041393 1102 50-51 0.5426846643257122 1102 52-53 0.5033843635956501 1102 54-55 0.5261927391825694 1102 56-57 0.6055119304205121 1102 58-59 0.35393894401649817 1102 60-61 0.3884928183521552 1102 62-63 0.4679473659933251 1102 64-65 0.5707897868815124 1102 66-67 0.7860319245609801 1102 68-69 0.7338640752161254 1102 70-71 0.733644901712136 1102 72-73 0.5610722239027446 1102 74-75 0.5732321362336492 1102 76-77 0.5590478157276131 1102 78-79 0.7789708865006872 1102 80-81 0.7150063061488723 1102 82-83 0.7473677718788672 1102 84-85 0.9845530225959607 1102 86-87 0.9190457907090241 1102 88-89 0.8584317545059115 1102 90-91 0.6560693939975479 1102 92-93 0.9208280481690636 1102 94-95 0.6572037461553073 1102 96-97 0.3380765692406946 1102 98-99 0.7175642568046001 1102 100-101 0.8031193075230298 1103 1 0.35235414171972934 1103 2 0.21142597610160863 1103 3 0.28640062454216064 1103 4 0.1582398527151625 1103 5 0.06980306572425832 1103 6 0.06351742836800156 1103 7 0.1329415730579342 1103 8 0.047284237407247076 1103 9 0.14432796103866252 1103 10-11 0.20583329984261667 1103 12-13 0.0217643062529973 1103 14-15 0.17198333602254223 1103 16-17 0.18365713859159172 1103 18-19 0.04690963366667944 1103 20-21 0.06713419399916631 1103 22-23 0.13217191290689811 1103 24-25 0.17415983353632924 1103 26-27 2.0832683851779166E-5 1103 28-29 0.13883007161522443 1103 30-31 0.13343513890409042 1103 32-33 0.36169306616535124 1103 34-35 0.19073848582248587 1103 36-37 0.16225545955111897 1103 38-39 0.35794057479220953 1103 40-41 0.4920037593388642 1103 42-43 0.46813039903999965 1103 44-45 0.3555185420965401 1103 46-47 0.7171077176034757 1103 48-49 0.48397343044845087 1103 50-51 0.3844928044239353 1103 52-53 0.6011969438092635 1103 54-55 0.39048533802697705 1103 56-57 0.4909112263256432 1103 58-59 0.4226952867349354 1103 60-61 0.6126107023957914 1103 62-63 0.5541071931484041 1103 64-65 0.5854793952776234 1103 66-67 0.5628047834207237 1103 68-69 0.6388461158673167 1103 70-71 0.4955199155237544 1103 72-73 0.17039051660018245 1103 74-75 0.4035090654824849 1103 76-77 0.5047915435546564 1103 78-79 0.3516072305110356 1103 80-81 0.426714135676999 1103 82-83 0.408350852863375 1103 84-85 0.4073380220774929 1103 86-87 0.20957161800349766 1103 88-89 0.43749708641813356 1103 90-91 0.40801704415317275 1103 92-93 0.3050331706207139 1103 94-95 0.05942516376243745 1103 96-97 -0.09548998914291573 1103 98-99 -0.0852330838858606 1103 100-101 -0.13454143842315602 1104 1 0.15359887834168262 1104 2 0.0968429332416818 1104 3 0.10379277757748895 1104 4 0.008374748563014123 1104 5 -0.022790559662652754 1104 6 0.03706111092007802 1104 7 0.11446397901426764 1104 8 0.028947813436921876 1104 9 0.11604514683939016 1104 10-11 0.06419265959276999 1104 12-13 0.19107515229744365 1104 14-15 0.19732336418685037 1104 16-17 0.18973790878762742 1104 18-19 0.12157727400019525 1104 20-21 -0.08701394046445188 1104 22-23 0.0652494553624905 1104 24-25 0.09611282564474521 1104 26-27 0.12691965731227128 1104 28-29 0.043558571935903956 1104 30-31 0.2028882007261288 1104 32-33 0.2753144096861675 1104 34-35 0.31231729009453346 1104 36-37 0.31044395122731316 1104 38-39 0.09219749735432714 1104 40-41 0.04611023991665064 1104 42-43 0.07120110956117998 1104 44-45 0.19618981450945938 1104 46-47 0.2406421631013771 1104 48-49 0.3638089411704115 1104 50-51 0.20935564288354414 1104 52-53 0.2576212278504215 1104 54-55 0.22925952865189458 1104 56-57 0.3566977004897822 1104 58-59 0.25640914022479677 1104 60-61 0.32774430854174597 1104 62-63 0.21587938299251874 1104 64-65 0.2688375350394736 1104 66-67 0.3635545533657947 1104 68-69 0.22705301193825278 1104 70-71 -0.07380156184720832 1104 72-73 0.011169845118626398 1104 74-75 0.09434660487067958 1104 76-77 -0.022909161947143986 1104 78-79 0.0015455339604670826 1104 80-81 0.03600504362713863 1104 82-83 0.09440429532175543 1104 84-85 0.0670188034277075 1104 86-87 0.03461309760529119 1104 88-89 0.14470338295197394 1104 90-91 0.24956757955374798 1104 92-93 0.5159613028535013 1104 94-95 0.310628595066639 1104 96-97 0.43682750060918707 1104 98-99 0.3661425400510083 1104 100-101 0.3302523138425393 1105 1 0.2422174434345905 1105 2 0.18913858810049078 1105 3 0.07788473289102882 1105 4 0.07182910773889972 1105 5 0.1445029899920911 1105 6 0.06346036988824721 1105 7 0.06955997631960287 1105 8 0.0123903834700414 1105 9 0.19237984621624804 1105 10-11 0.22537410011373282 1105 12-13 0.26038373310921514 1105 14-15 0.24067828755742227 1105 16-17 0.12607302920530117 1105 18-19 0.12663502676502958 1105 20-21 -0.054009603744077594 1105 22-23 -0.07862545341200189 1105 24-25 0.16163203914050683 1105 26-27 0.05142879389791233 1105 28-29 -0.03539886083790833 1105 30-31 0.012919848960564195 1105 32-33 0.19417282769548905 1105 34-35 -0.07225787972526376 1105 36-37 -0.06499463295278929 1105 38-39 0.14335746479383005 1105 40-41 0.18382918113935176 1105 42-43 0.035358256027244295 1105 44-45 0.17650966518032618 1105 46-47 0.209430758509221 1105 48-49 0.4025008375382342 1105 50-51 0.15266074309126054 1105 52-53 0.07770873416603052 1105 54-55 -0.04489593365092048 1105 56-57 -0.03321240729666641 1105 58-59 -0.07325529024732447 1105 60-61 -0.351361190251275 1105 62-63 -0.3875738534078579 1105 64-65 -0.2044070756381231 1105 66-67 -0.49970191662803387 1105 68-69 -0.35172021547992216 1105 70-71 -0.31260538489778966 1105 72-73 -0.20651878116554556 1105 74-75 -0.2682888299241313 1105 76-77 -0.6222958458297008 1105 78-79 -0.47325868206153743 1105 80-81 -0.3242532649115688 1105 82-83 -0.3240716772825216 1105 84-85 -0.31976683065975564 1105 86-87 -0.5429882328836761 1105 88-89 -0.38756939535600665 1105 90-91 -0.13531109051866963 1105 92-93 -0.2110270623853694 1105 94-95 -0.3042681797607365 1105 96-97 -0.33607361093855914 1105 98-99 -0.12790850413021104 1105 100-101 -0.03810050432397105 1106 1 0.20072595339429 1106 2 0.144354122660161 1106 3 0.08139723998438342 1106 4 0.0967273154490158 1106 5 0.14623646194795725 1106 6 0.04171223487211506 1106 7 0.08287892273315833 1106 8 -0.059667788286581924 1106 9 0.043021665761202144 1106 10-11 0.009068072933224869 1106 12-13 -0.1141652949452805 1106 14-15 0.09142365978789968 1106 16-17 0.11717578246410199 1106 18-19 0.022706444981189122 1106 20-21 0.19281650644425952 1106 22-23 0.16770256835891928 1106 24-25 0.10257283599913336 1106 26-27 0.27580468186219065 1106 28-29 0.1715594654193211 1106 30-31 0.20512295305586292 1106 32-33 0.17330281945269377 1106 34-35 -0.22158706542835915 1106 36-37 0.14979020527721332 1106 38-39 0.2435281196195902 1106 40-41 0.21959006556212302 1106 42-43 0.042007373965468275 1106 44-45 -0.07172786811187137 1106 46-47 -0.31137297601483027 1106 48-49 -0.24405934935365536 1106 50-51 0.11061006854906452 1106 52-53 0.3651492431481884 1106 54-55 -0.06740932301315894 1106 56-57 -0.17235407979679707 1106 58-59 -0.1847608220317838 1106 60-61 -0.241558064617017 1106 62-63 -0.34089230276852334 1106 64-65 -0.44591057778993815 1106 66-67 -0.5990130841638504 1106 68-69 -0.17465754953674661 1106 70-71 0.06618516115623763 1106 72-73 -0.15421017759422284 1106 74-75 -0.04565055602314061 1106 76-77 -0.043830869419000607 1106 78-79 -0.01645982694003223 1106 80-81 0.11403140556307534 1106 82-83 -0.3234265440516353 1106 84-85 -0.6181973861577816 1106 86-87 -0.16200961858429608 1106 88-89 -0.29780889200710803 1106 90-91 -0.20036081651826976 1106 92-93 -0.3768760080119229 1106 94-95 -0.28472675247335744 1106 96-97 -0.1026047616574246 1106 98-99 -0.2862830169148225 1106 100-101 -0.3450969379694797 1107 1 0.14456522911129355 1107 2 0.10402223579053782 1107 3 0.06946220060081032 1107 4 0.08809423507545233 1107 5 0.026808374959045977 1107 6 0.02063910961932436 1107 7 0.08132962985510517 1107 8 0.04490719588915226 1107 9 0.15776988281758975 1107 10-11 0.1724144998479602 1107 12-13 0.048951480149042936 1107 14-15 0.1792260632484428 1107 16-17 0.1401912365463076 1107 18-19 0.11279622627495911 1107 20-21 0.14112199592255337 1107 22-23 0.17435104568736648 1107 24-25 0.045862546912054825 1107 26-27 0.06479807565908402 1107 28-29 0.07115020530950744 1107 30-31 0.20791990657022552 1107 32-33 -0.01778463333157987 1107 34-35 -0.0787504884058805 1107 36-37 -0.07993473810961405 1107 38-39 -0.041390953821000664 1107 40-41 -0.2555424126895005 1107 42-43 -0.08722261833376876 1107 44-45 -0.06481432002551202 1107 46-47 0.027950727937700037 1107 48-49 -0.017037670678824668 1107 50-51 -0.29116407745465267 1107 52-53 -0.20422045972119918 1107 54-55 -0.13497151752481784 1107 56-57 -0.14248449003950014 1107 58-59 -0.19687409864912553 1107 60-61 -0.16642650040248697 1107 62-63 -0.4192149989951517 1107 64-65 -0.1017927492678723 1107 66-67 -0.36627410345231937 1107 68-69 -0.11837228769939401 1107 70-71 -0.12441670942068228 1107 72-73 -0.13280914153317624 1107 74-75 -0.2608091757357158 1107 76-77 -0.1402594979748919 1107 78-79 -0.29464473207440633 1107 80-81 -0.206249478036586 1107 82-83 -0.1882713062763095 1107 84-85 -0.061091776026593436 1107 86-87 -0.07178730191157356 1107 88-89 -0.3636469840460741 1107 90-91 -0.4043693262036321 1107 92-93 -0.63476243433254 1107 94-95 -0.5765707994640046 1107 96-97 -0.28167376917309284 1107 98-99 -0.3917674680012695 1107 100-101 -0.3705620114694881 1108 1 0.1940095230772556 1108 2 0.09548900463576615 1108 3 -0.027552379663312365 1108 4 0.10924343559427285 1108 5 0.1404533969472581 1108 6 0.009837690570506652 1108 7 -0.031176893937740147 1108 8 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82-83 -0.4349650055900973 2316 84-85 -0.450867890256859 2316 86-87 -0.5364819716280742 2316 88-89 -0.3308369750351936 2316 90-91 -0.028199644357094655 2316 92-93 -0.006267251688882425 2316 94-95 -0.12371157717246817 2316 96-97 0.07143151952171323 2316 98-99 0.23515427595103944 2316 100-101 0.7299335870610477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 4.0 5 20.0 6 29.0 7 73.0 8 171.0 9 336.0 10 651.0 11 1051.0 12 1688.0 13 2533.0 14 3423.0 15 5014.0 16 7145.0 17 10389.0 18 9069.0 19 4903.0 20 3735.0 21 3482.0 22 3546.0 23 3833.0 24 4387.0 25 5090.0 26 5987.0 27 7417.0 28 9080.0 29 11417.0 30 14311.0 31 18202.0 32 23527.0 33 30847.0 34 43865.0 35 64471.0 36 98509.0 37 149642.0 38 113502.0 39 9064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73275966242132 19.669761287059735 15.575344284599652 21.022134765919294 2 15.536426527927352 20.6662112294121 41.0399183792798 22.757443863380754 3 19.837027048796422 25.556145568883892 30.334060243012566 24.27276713930712 4 12.371982852489044 17.09232150688078 37.67574856283692 32.85994707779326 5 12.721448064805408 38.50141228396084 33.914063304341596 14.863076346892154 6 30.650728351565736 37.04748380860895 17.262662995325304 15.039124844499998 7 27.48016908853879 34.52010536709933 20.21559683660045 17.784128707761425 8 26.68245780990367 34.94252839273408 19.22263423714757 19.152379560214673 9 26.90455805184228 16.650060111751174 20.587901876142944 35.857479960263596 10-11 24.799453475294516 27.57540817818137 26.542918000411685 21.082220346112425 12-13 26.117668644119625 25.036732295033794 27.99224631595591 20.853352744890667 14-15 22.819942185323185 25.66682278817096 27.616278202554227 23.896956823951623 16-17 20.731767345148846 29.247275580309594 28.190472212858246 21.83048486168331 18-19 20.6029671041052 26.768374357490405 32.30857465044196 20.320083887962433 20-21 21.5228409738402 25.50080695924035 32.7956588277761 20.18069323914334 22-23 21.067952041574312 25.631475476347447 32.71769384290901 20.58287863916923 24-25 20.78032205400979 25.903711951767228 32.92980248815846 20.386163506064523 26-27 20.650690397383098 26.124571345040582 32.720152264804724 20.5045859927716 28-29 20.356646094755096 26.29507952193612 32.97454925139373 20.37372513191505 30-31 20.57410451390028 26.121389938814293 32.772986405496276 20.531519141789154 32-33 20.39578292945595 26.12661056236557 32.68266961805918 20.7949368901193 34-35 20.903451279649413 26.122061161842314 32.576094317276684 20.39839324123159 36-37 20.954837131461268 25.936356124089748 32.62434779495778 20.484458949491213 38-39 20.732289407503977 26.238406486699343 32.52217273402564 20.507131371771045 40-41 20.800729101719238 26.291664553544525 32.52788575417578 20.37972059056046 42-43 20.688693234199857 26.37027069764389 32.12419928931799 20.816836778838265 44-45 20.713047680700697 26.720046299472866 31.7472084580068 20.81969756181964 46-47 21.13218917153179 26.969368364214564 30.989024757688487 20.909417706565158 48-49 21.125999003606704 27.23054870245131 30.596060964950222 21.047391328991765 50-51 21.367564713357933 27.48747796151058 30.399243457067083 20.745713868064403 52-53 21.36816135604951 27.61829187163829 30.05893338186027 20.954613390451925 54-55 21.050091920333223 27.602128524389652 30.24637632520034 21.10140323007678 56-57 21.30961579193876 27.819584199708242 29.835117792183386 21.03568221616961 58-59 21.248459916052372 27.51186573152869 29.967647050049372 21.272027302369565 60-61 21.136887732727942 27.565712734443288 30.314743935872844 20.98265559695593 62-63 21.228099484202392 27.54505398124752 30.378957605553552 20.847888928996536 64-65 21.059547923832593 27.495681798519726 30.33376192166678 21.111008355980896 66-67 20.99317142439493 27.714798827000468 30.030891175356274 21.26113857324833 68-69 21.319758717695528 27.536328081883244 30.031338657374953 21.11257454304628 70-71 21.243015551491755 27.595768610031353 29.831239614688148 21.32997622378874 72-73 21.195060395156453 27.691007699673936 29.74487558508274 21.36905632008687 74-75 21.109442168915514 28.040714897273045 29.369512751745923 21.48033018206552 76-77 21.1425558382979 27.616203622217782 29.810730022165277 21.430510517319046 78-79 21.122046245775024 27.74343767619604 29.678051603626393 21.456464474402537 80-81 21.089976701102895 28.045040556786958 29.455429299332653 21.40955344277749 82-83 21.076454250657058 27.936543687930143 29.559570653357476 21.427431408055323 84-85 21.100716269551235 27.9504726901724 29.31134008931741 21.637470950958953 86-87 21.009280777067442 27.86194583081003 29.31857438195275 21.810199010169775 88-89 21.044930177889018 27.741125685766193 29.310743446625835 21.90320068971895 90-91 20.927226413757353 27.801053954567323 29.403467682083146 21.868251949592178 92-93 20.926361260605482 27.659052397942745 29.48741661794012 21.927169723511653 94-95 20.74765295681202 27.664158778553084 29.409264071072077 22.17892419356282 96-97 20.82767765781945 27.602257099302225 29.42604464677261 22.14402059610571 98-99 20.794081826107774 27.48372449972741 29.181343641460927 22.540850032703894 100-101 20.670131854163746 27.4070322220664 29.493156652533397 22.42967927123646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 429.0 1 305.5 2 246.5 3 333.5 4 292.5 5 238.0 6 446.5 7 991.5 8 1579.5 9 1877.0 10 1699.0 11 1425.0 12 1429.0 13 1504.0 14 1658.5 15 1810.5 16 1853.0 17 1918.0 18 2018.5 19 2126.0 20 2276.5 21 2558.5 22 2897.0 23 3312.5 24 3853.0 25 4534.0 26 5481.0 27 6634.0 28 7509.5 29 8493.0 30 9756.5 31 10986.5 32 12462.0 33 13630.0 34 14679.0 35 15883.0 36 17623.0 37 19117.0 38 19812.5 39 20369.0 40 21049.5 41 21847.5 42 22244.0 43 23016.0 44 24162.5 45 24800.5 46 24609.5 47 24810.0 48 26858.0 49 25999.5 50 23262.0 51 21927.0 52 20108.5 53 18982.5 54 17984.5 55 16508.0 56 14867.0 57 13126.0 58 11899.0 59 10572.0 60 8661.5 61 7024.5 62 5587.0 63 4078.0 64 2831.5 65 2008.5 66 1456.0 67 1121.0 68 814.5 69 563.5 70 410.0 71 300.0 72 242.0 73 211.5 74 183.0 75 140.0 76 107.5 77 64.5 78 43.0 79 31.5 80 18.0 81 15.5 82 14.0 83 8.0 84 7.0 85 7.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01715347738276717 2 0.0 3 0.0 4 0.0 5 0.033859472746853456 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004027338168127944 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.966426915745102E-4 24-25 3.729016822340689E-4 26-27 0.001044124710255393 28-29 8.203837009149515E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.966426915745102E-4 42-43 0.011336211139915694 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.458033644681378E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.966426915745102E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012753237532405157 92-93 0.0014916067289362756 94-95 0.0 96-97 0.0 98-99 0.0012678657195958343 100-101 0.019689208821958838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 670418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.89743018392085 #Duplication Level Percentage of deduplicated Percentage of total 1 82.96268686325024 55.49990552304771 2 10.756945123513152 14.392239707849777 3 2.9188687758376073 5.8579446044286865 4 1.168608459212005 3.127076112498976 5 0.5998151262873521 2.0063045267033908 6 0.36659455490190473 1.4714540185413427 7 0.22491914562778412 1.053255898916327 8 0.17323477464524978 0.9271168993806287 9 0.11885803712093643 0.7156167516086146 >10 0.6505298196092243 7.808880075521629 >50 0.036080822105740075 1.6654603790783897 >100 0.020841571604588525 2.634528252266332 >500 0.0011205146023108145 0.49308390265348084 >1k 6.723087613864886E-4 1.448818131724913 >5k 2.2410292046216287E-4 0.898315215779768 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5992 0.8937707519786163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4930 0.7353621173655839 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3495 0.5213165517632283 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.18481007371520455 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 940 0.1402110325200099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.4916067289362755E-4 0.0 0.0 0.0 0.0 6 1.4916067289362755E-4 0.0 0.0 0.0 0.0 7 1.4916067289362755E-4 0.0 0.0 0.0 0.0 8 1.4916067289362755E-4 0.0 0.0 0.0 0.0 9 1.4916067289362755E-4 0.0 0.0 0.0 0.0 10-11 2.983213457872551E-4 0.0 0.0 0.0 0.0 12-13 2.983213457872551E-4 0.0 0.0 7.458033644681378E-5 0.0 14-15 4.4748201868088266E-4 0.0 0.0 2.2374100934044133E-4 0.0 16-17 5.966426915745102E-4 0.0 0.0 4.4748201868088266E-4 0.0 18-19 7.458033644681378E-4 0.0 0.0 4.4748201868088266E-4 0.0 20-21 7.458033644681378E-4 0.0 0.0 4.4748201868088266E-4 0.0 22-23 7.458033644681378E-4 0.0 0.0 5.966426915745102E-4 0.0 24-25 8.949640373617653E-4 0.0 0.0 6.712230280213241E-4 0.0 26-27 8.949640373617653E-4 0.0 0.0 9.695443738085791E-4 0.0 28-29 9.695443738085791E-4 0.0 0.0 0.001715347738276717 0.0 30-31 0.001044124710255393 0.0 0.0 0.0038781774952343162 0.0 32-33 0.0011187050467022066 0.0 0.0 0.006264748261532357 0.0 34-35 0.0013424460560426481 0.0 0.0 0.008129256672702702 0.0 36-37 0.0014916067289362756 0.0 0.0 0.010739568448341185 0.0 38-39 0.0014916067289362756 0.0 0.0 0.014766906616469129 7.458033644681378E-5 40-41 0.0014916067289362756 0.0 0.0 0.018794244784597073 1.4916067289362755E-4 42-43 0.0015661870653830893 0.0 0.0 0.027520144148874284 1.4916067289362755E-4 44-45 0.0016407674018299031 0.0 0.0 0.040944604709300766 1.4916067289362755E-4 46-47 0.0018645084111703445 0.0 0.0 0.054816547288408124 1.4916067289362755E-4 48-49 0.0019390887476171583 0.0 0.0 0.08509616388581452 1.4916067289362755E-4 50-51 0.0019390887476171583 0.0 0.0 0.11559952149256136 1.4916067289362755E-4 52-53 0.002013669084063972 0.0 0.0 0.14431295102458466 2.2374100934044133E-4 54-55 0.002088249420510786 0.0 0.0 0.18466091304231091 2.983213457872551E-4 56-57 0.002088249420510786 0.0 0.0 0.23067698062999503 2.983213457872551E-4 58-59 0.002088249420510786 0.0 0.0 0.2866868133015522 2.983213457872551E-4 60-61 0.002088249420510786 1.4916067289362755E-4 0.0 0.3573889722531316 2.983213457872551E-4 62-63 0.002088249420510786 1.4916067289362755E-4 0.0 0.4603098365497347 2.983213457872551E-4 64-65 0.002088249420510786 1.4916067289362755E-4 0.0 0.5448093577439747 2.983213457872551E-4 66-67 0.0022374100934044136 1.4916067289362755E-4 0.0 0.663615833703749 2.983213457872551E-4 68-69 0.0022374100934044136 1.4916067289362755E-4 0.0 0.8071084010274188 2.983213457872551E-4 70-71 0.0022374100934044136 1.4916067289362755E-4 0.0 0.9215892174732778 2.983213457872551E-4 72-73 0.0023119904298512274 1.4916067289362755E-4 0.0 1.0736585234883311 4.4748201868088266E-4 74-75 0.0025357314391916685 1.4916067289362755E-4 0.0 1.2636892207548125 4.4748201868088266E-4 76-77 0.002684892112085296 1.4916067289362755E-4 0.0 1.425677711517292 4.4748201868088266E-4 78-79 0.0027594724485321097 1.4916067289362755E-4 0.0 1.5943038522235382 4.4748201868088266E-4 80-81 0.003729016822340689 1.4916067289362755E-4 0.0 1.8292319120310014 4.4748201868088266E-4 82-83 0.0038781774952343167 1.4916067289362755E-4 0.0 2.091456374977999 4.4748201868088266E-4 84-85 0.0039527578316811305 1.4916067289362755E-4 0.0 2.35382999859789 4.4748201868088266E-4 86-87 0.004101918504574758 1.4916067289362755E-4 0.0 2.696824965916786 4.4748201868088266E-4 88-89 0.004176498841021572 1.4916067289362755E-4 0.0 3.082629046356154 4.4748201868088266E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3490 0.0 39.484642 1 CGTGCGA 25 0.0016153204 38.00283 10-11 GTATCAA 5530 0.0 35.316063 1 AGGACCG 135 3.074092E-10 35.198315 5 GCCGGTC 60 0.003948345 31.669027 8 ACCGAGT 155 1.3533281E-9 30.647448 8 GGACCGA 140 1.7049388E-8 30.533438 6 TATCAAC 7065 0.0 27.643005 2 ATCAACG 7120 0.0 27.42947 3 TCAACGC 7155 0.0 27.228882 4 CAACGCA 7220 0.0 27.115376 5 AACGCAG 7405 0.0 26.426117 6 ACGCAGA 8165 0.0 23.911766 7 GAGTACT 4895 0.0 23.67899 12-13 CGCAGAG 8195 0.0 23.65033 8 AGTACTT 5095 0.0 22.889341 12-13 GTACTTT 5155 0.0 22.343143 14-15 GCAGAGT 8640 0.0 22.322268 9 GTACATG 4155 0.0 22.300735 1 TACATGG 4175 0.0 22.193905 2 >>END_MODULE