FastQCFastQC Report
Thu 26 May 2016
SRR937658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682712
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45030.6595753407000317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.5018221446232086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.3471449161579114No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9160.1341707777217919No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.1318271833511056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.030.194181
CCGTCTA650.005839688229.2335689
GTATCAA54500.028.6151031
GTACATG43850.027.4327221
TACATGG44200.026.7812732
ACATGGG43550.025.8672663
AGGACCG2251.0186341E-1025.352515
GTGTAAG3100.024.5400961
GTGTAGC4800.023.7732181
TATCAAC66050.023.5358072
ATCAACG66400.023.408313
TCAACGC66800.023.341014
CAACGCA67200.023.1313385
GAGTACT36850.023.07209412-13
CATGGGA28400.022.9310784
AACGCAG69100.022.5475396
AGCGTCG753.0246363E-522.17530326-27
GTACTTT38750.021.57230214-15
AGTACTT38650.021.26023712-13
GTAGCCC3151.4551915E-1121.125543
Produced by FastQC (version 0.11.5)