##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937658_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682712 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784080256389224 33.0 31.0 34.0 30.0 34.0 2 31.895758387138354 33.0 31.0 34.0 30.0 34.0 3 31.930505103176742 33.0 31.0 34.0 30.0 34.0 4 35.51311680474343 37.0 35.0 37.0 33.0 37.0 5 35.512173508009234 37.0 35.0 37.0 33.0 37.0 6 35.60298046614092 37.0 35.0 37.0 33.0 37.0 7 35.5534134452009 37.0 35.0 37.0 33.0 37.0 8 35.54382374998535 37.0 35.0 37.0 33.0 37.0 9 37.16030624919439 39.0 37.0 39.0 34.0 39.0 10-11 37.060826527144684 39.0 37.0 39.0 33.0 39.0 12-13 36.93846453555818 39.0 37.0 39.0 33.0 39.0 14-15 38.06456968677861 40.0 38.0 41.0 33.0 41.0 16-17 38.01779081076647 40.0 38.0 41.0 33.0 41.0 18-19 38.01683945792662 40.0 38.0 41.0 33.0 41.0 20-21 37.82866567454505 40.0 38.0 41.0 33.0 41.0 22-23 37.721218464008246 40.0 37.5 41.0 32.5 41.0 24-25 37.5774184429159 40.0 37.0 41.0 32.0 41.0 26-27 37.675998810625856 40.0 37.0 41.0 33.0 41.0 28-29 37.609475884413925 40.0 37.0 41.0 32.5 41.0 30-31 37.37156077526102 39.5 37.0 41.0 32.0 41.0 32-33 37.10957621954792 39.0 36.0 41.0 31.5 41.0 34-35 36.84286199744548 39.0 36.0 41.0 31.0 41.0 36-37 36.84569188764809 39.0 36.0 41.0 31.0 41.0 38-39 36.78713059093732 39.0 36.0 41.0 31.0 41.0 40-41 36.57352807626056 39.0 35.5 41.0 30.0 41.0 42-43 36.296341649187355 39.0 35.0 41.0 30.0 41.0 44-45 36.01410111437912 39.0 35.0 41.0 29.5 41.0 46-47 35.58004693047727 39.0 35.0 41.0 27.5 41.0 48-49 35.18008179144354 39.0 35.0 40.0 25.5 41.0 50-51 34.253377705386754 38.0 34.0 39.5 23.5 40.5 52-53 34.32905456473594 38.0 34.0 39.5 24.0 40.5 54-55 34.62625382298832 38.0 34.0 40.0 24.0 41.0 56-57 34.38535941949168 38.0 34.0 40.0 22.5 41.0 58-59 34.15320369350473 38.0 34.0 40.0 22.0 41.0 60-61 33.84897072264732 38.0 34.0 40.0 21.5 41.0 62-63 33.503808340852366 37.0 33.0 40.0 19.5 41.0 64-65 33.146791765781174 37.0 33.0 40.0 18.0 41.0 66-67 32.73878956280247 36.0 32.5 39.0 16.0 41.0 68-69 32.274223244940764 36.0 32.0 39.0 12.0 40.5 70-71 31.842797548600288 35.0 31.0 38.5 8.0 40.0 72-73 31.37506298409871 35.0 31.0 38.0 7.0 40.0 74-75 30.896622587562547 35.0 30.5 37.0 3.5 39.0 76-77 30.448772688923 35.0 30.0 37.0 2.0 39.0 78-79 29.928751069264933 34.0 29.5 36.0 2.0 39.0 80-81 29.42215385111145 34.0 29.0 36.0 2.0 37.5 82-83 28.90472556509919 34.0 29.0 35.0 2.0 37.0 84-85 28.43785739814153 34.0 28.5 35.0 2.0 36.5 86-87 27.921180527074377 34.0 27.0 35.0 2.0 36.0 88-89 27.436370680462623 33.0 26.0 35.0 2.0 36.0 90-91 26.933875484831084 33.0 25.5 35.0 2.0 35.0 92-93 26.3960586601671 33.0 25.0 35.0 2.0 35.0 94-95 25.77094001570208 32.0 23.5 34.5 2.0 35.0 96-97 25.002131938504085 32.0 19.0 34.0 2.0 35.0 98-99 24.078965947573792 31.5 4.0 34.0 2.0 35.0 100-101 21.577779502923633 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.034708687422316586 1101 2 0.10130216286177784 1101 3 0.18126361774755395 1101 4 0.07057468693794533 1101 5 -0.06111940499919655 1101 6 -0.060617269597734946 1101 7 -0.03309381174008763 1101 8 0.09244546278626586 1101 9 -0.038198128588447844 1101 10-11 -0.0236300187777303 1101 12-13 0.15749513356974632 1101 14-15 0.41420966750147414 1101 16-17 0.24311406758161525 1101 18-19 0.12032341969680971 1101 20-21 0.36161370009080684 1101 22-23 0.14005234512448084 1101 24-25 0.2998509371172986 1101 26-27 0.3110161680397283 1101 28-29 0.11985784207805494 1101 30-31 0.025430616946515272 1101 32-33 0.1732719951980144 1101 34-35 0.2651758233398027 1101 36-37 0.18766554553049986 1101 38-39 -0.1357587046999953 1101 40-41 -0.10672703954997331 1101 42-43 -0.008745532802251432 1101 44-45 0.17857299917551472 1101 46-47 -0.12548381368192452 1101 48-49 0.1931239792748869 1101 50-51 0.1196746162344624 1101 52-53 0.03488113834598039 1101 54-55 -0.045123918239518446 1101 56-57 0.22715416780844322 1101 58-59 0.35027748596343855 1101 60-61 0.5016930996650046 1101 62-63 0.2148350313848013 1101 64-65 0.2745228379296094 1101 66-67 0.3840653445178148 1101 68-69 0.2538069981793356 1101 70-71 0.13673775025386448 1101 72-73 0.1324465218240256 1101 74-75 0.10973962589413588 1101 76-77 0.45406855397227375 1101 78-79 0.4272967316948275 1101 80-81 0.5849098812027016 1101 82-83 0.608740714760188 1101 84-85 0.5127413537528547 1101 86-87 0.643898176209877 1101 88-89 0.709714746485961 1101 90-91 0.7406142972683121 1101 92-93 0.852810795594241 1101 94-95 1.0136242593720333 1101 96-97 1.2462530541987995 1101 98-99 1.6629296753263496 1101 100-101 1.935492178088662 1102 1 0.1889086019846431 1102 2 0.275102053123387 1102 3 0.2435983088996565 1102 4 0.06783396373182882 1102 5 0.1361346722199599 1102 6 0.21719327158221802 1102 7 0.15369133225392773 1102 8 0.1098065691463006 1102 9 0.15367302047651776 1102 10-11 0.09869476581619807 1102 12-13 0.17681155368990886 1102 14-15 0.34266830830527795 1102 16-17 0.31387412225925004 1102 18-19 0.1456056676645332 1102 20-21 0.04612471429522458 1102 22-23 0.3254698641337157 1102 24-25 0.43170188993936165 1102 26-27 0.3723882537123018 1102 28-29 0.30225960774416194 1102 30-31 0.21501121651221666 1102 32-33 0.4725143624275603 1102 34-35 0.3309627584504753 1102 36-37 0.41628058406893587 1102 38-39 0.5559024089120825 1102 40-41 0.6360198957796328 1102 42-43 0.7772050794476755 1102 44-45 0.7057736165565984 1102 46-47 0.7820869039551681 1102 48-49 0.6173004966271094 1102 50-51 0.7932165802233015 1102 52-53 0.8570618399875443 1102 54-55 0.9481234761577966 1102 56-57 1.1080695392564834 1102 58-59 1.1538402893004687 1102 60-61 1.1492659244981667 1102 62-63 1.0766919089854312 1102 64-65 0.916306346794201 1102 66-67 0.8533023342924437 1102 68-69 0.9166535737804722 1102 70-71 1.021792256848432 1102 72-73 1.222276700976746 1102 74-75 1.0422245163404646 1102 76-77 1.1131892237869643 1102 78-79 1.2091239443533262 1102 80-81 0.9901230021926999 1102 82-83 1.0551303626805577 1102 84-85 1.270157247210829 1102 86-87 1.2370868701451165 1102 88-89 1.3207824517904854 1102 90-91 1.509315320353057 1102 92-93 1.3129615285290335 1102 94-95 1.5133998566731783 1102 96-97 1.6962577748653267 1102 98-99 2.0249972725344527 1102 100-101 2.028717816370669 1103 1 0.021902267493391747 1103 2 0.09143407080403776 1103 3 0.2093232263576823 1103 4 0.08431278244062668 1103 5 0.07999403360859958 1103 6 0.2538545429974235 1103 7 0.21768824566820655 1103 8 0.10405686221062638 1103 9 0.2395906075623273 1103 10-11 0.239679542603362 1103 12-13 0.20050204012291317 1103 14-15 0.40807475356946554 1103 16-17 0.35442320112312586 1103 18-19 0.11667497603272636 1103 20-21 0.35807152177616075 1103 22-23 0.16456074633798323 1103 24-25 0.14177254801664674 1103 26-27 0.08431745044718753 1103 28-29 0.06353720687518916 1103 30-31 0.09261115413521992 1103 32-33 0.08714149857072329 1103 34-35 0.149543843164885 1103 36-37 0.21697533472227803 1103 38-39 0.1941729798766687 1103 40-41 0.15415022605505868 1103 42-43 0.12795199017328684 1103 44-45 0.20450591170732224 1103 46-47 0.3108139293253984 1103 48-49 0.09531193420171746 1103 50-51 -0.03871909619066827 1103 52-53 -0.23470224592063005 1103 54-55 -0.1653266975298635 1103 56-57 0.14075696599039844 1103 58-59 0.005961779250249322 1103 60-61 0.1665842783908147 1103 62-63 -0.05401074633865477 1103 64-65 0.23136729879618656 1103 66-67 0.33134939665868046 1103 68-69 0.34474930095640133 1103 70-71 0.2715919514162515 1103 72-73 0.32100778622888626 1103 74-75 0.3589026994492066 1103 76-77 0.23661769996002846 1103 78-79 0.20120851895298486 1103 80-81 0.42826155977849467 1103 82-83 0.1979091502008572 1103 84-85 0.28437086690475155 1103 86-87 0.26955382647046733 1103 88-89 0.3136561491330099 1103 90-91 0.4637041602485219 1103 92-93 0.4895583257930518 1103 94-95 0.26450355304778483 1103 96-97 0.622017053443134 1103 98-99 0.7043620488914222 1103 100-101 0.9998778671326356 1104 1 0.06642744084280494 1104 2 0.2323565911537493 1104 3 0.1584459275937391 1104 4 0.2167125412588362 1104 5 0.22057136252026766 1104 6 0.2043806207785508 1104 7 0.12594419984366567 1104 8 0.2600155598289575 1104 9 0.3338489735556962 1104 10-11 0.20923561736971408 1104 12-13 0.18810002022541283 1104 14-15 0.3980890737382978 1104 16-17 0.2807466862000041 1104 18-19 0.3611511593308805 1104 20-21 0.4517314554511742 1104 22-23 0.31823500018182216 1104 24-25 0.1997044141607489 1104 26-27 0.21300074860818086 1104 28-29 0.10324628134006986 1104 30-31 0.1082321972464726 1104 32-33 0.26798412745224454 1104 34-35 0.31203555254082715 1104 36-37 0.20909471970870896 1104 38-39 0.1906223317006024 1104 40-41 0.21873343377287569 1104 42-43 0.16330924070087605 1104 44-45 0.1384778743241455 1104 46-47 0.1659073872693213 1104 48-49 0.1990585425827831 1104 50-51 0.08196374975446474 1104 52-53 0.20334418157409573 1104 54-55 0.17982027262389266 1104 56-57 0.41309793674710704 1104 58-59 0.4170267475951377 1104 60-61 0.5833109892741462 1104 62-63 0.42861662779459664 1104 64-65 0.09715641546787879 1104 66-67 0.3234838555070354 1104 68-69 0.29454881859282267 1104 70-71 0.30768631379190126 1104 72-73 0.22225665568923958 1104 74-75 -0.04683818086433078 1104 76-77 -0.22179351499324085 1104 78-79 -0.07716953350405475 1104 80-81 0.3291682525731865 1104 82-83 0.266748462832755 1104 84-85 0.22233438815889528 1104 86-87 -0.0021269091339952695 1104 88-89 -0.10213673600416229 1104 90-91 0.47591021994101723 1104 92-93 0.4735257662512957 1104 94-95 0.5444477797586664 1104 96-97 0.6709272132260686 1104 98-99 1.1619607826870215 1104 100-101 1.225037650069023 1105 1 -0.09948928732981699 1105 2 0.13412535770608613 1105 3 0.051262321463408256 1105 4 0.060932573386651256 1105 5 0.17650068132372354 1105 6 0.0336481933917554 1105 7 0.07308786328307804 1105 8 0.14519942645510042 1105 9 0.019572934828651967 1105 10-11 0.2828291570736141 1105 12-13 0.2937658041007225 1105 14-15 0.3359365100253555 1105 16-17 0.3279502636758025 1105 18-19 0.39906371957054176 1105 20-21 0.48248819578026314 1105 22-23 0.560378880701073 1105 24-25 0.4576239209553563 1105 26-27 0.5533940972818243 1105 28-29 0.27056447697241737 1105 30-31 0.25475848561533354 1105 32-33 0.42004768842996754 1105 34-35 0.3666684705280616 1105 36-37 0.4365448998849786 1105 38-39 0.4770444217453189 1105 40-41 0.6287173698645745 1105 42-43 0.6854418330683458 1105 44-45 0.5308870264102907 1105 46-47 0.6142519363944885 1105 48-49 0.482649043168891 1105 50-51 0.4896909238090785 1105 52-53 0.15209163088594835 1105 54-55 0.1736352337665963 1105 56-57 0.5649010019144711 1105 58-59 0.34013446261271696 1105 60-61 0.3225642346177864 1105 62-63 0.42501310241797086 1105 64-65 0.43066746483182783 1105 66-67 0.5957957558239784 1105 68-69 0.7500413727542465 1105 70-71 0.759694302330086 1105 72-73 0.510685431793096 1105 74-75 0.24748822038604956 1105 76-77 0.4884352871601898 1105 78-79 0.4085518228107965 1105 80-81 0.5294513246871126 1105 82-83 0.5563260254602902 1105 84-85 0.6669399540860432 1105 86-87 0.40239963973067816 1105 88-89 0.6248393417972515 1105 90-91 0.7372439584900512 1105 92-93 0.8517361600341289 1105 94-95 0.608082564632543 1105 96-97 0.6871152912011489 1105 98-99 1.0606704601063761 1105 100-101 1.187785880868315 1106 1 0.17137560613524983 1106 2 0.19944854351403407 1106 3 0.22049906419627519 1106 4 0.3074768018307026 1106 5 0.34422052593219377 1106 6 0.2840450211835801 1106 7 0.36310510441958144 1106 8 0.14610071340434416 1106 9 0.22175292263713686 1106 10-11 0.10899645443996775 1106 12-13 -0.006779192851396942 1106 14-15 0.17831087806829515 1106 16-17 0.3050207592025629 1106 18-19 0.05845671890462967 1106 20-21 -0.04680463597078699 1106 22-23 -0.06537076673075859 1106 24-25 0.09573508102029393 1106 26-27 0.10008498603524885 1106 28-29 0.3714472553503825 1106 30-31 0.5433553950862873 1106 32-33 0.6482261834819454 1106 34-35 0.26405252539390744 1106 36-37 0.31319231361391786 1106 38-39 0.6170064389381764 1106 40-41 0.3722982151027381 1106 42-43 0.7139500623412616 1106 44-45 0.43067518374118663 1106 46-47 0.6254888521422686 1106 48-49 0.6885961287730495 1106 50-51 0.7498811999690389 1106 52-53 0.6920610917433407 1106 54-55 0.4773669238606928 1106 56-57 0.6236596803220138 1106 58-59 0.4993846240597648 1106 60-61 -0.013330121676233375 1106 62-63 0.38256812015199415 1106 64-65 0.5315110130858898 1106 66-67 0.6582531307855035 1106 68-69 0.6462716596169784 1106 70-71 0.335257556378334 1106 72-73 0.4599749641901347 1106 74-75 0.5724424016131273 1106 76-77 0.513781970806459 1106 78-79 0.503521917385573 1106 80-81 0.28796375862458845 1106 82-83 0.1491545576299984 1106 84-85 0.10566048874234113 1106 86-87 -0.021526108709672087 1106 88-89 0.23293342166978448 1106 90-91 0.49866445109970314 1106 92-93 0.6536964591487369 1106 94-95 0.5005739189167748 1106 96-97 0.32572638387074804 1106 98-99 0.270685407789486 1106 100-101 -0.02416240779297496 1107 1 0.23982920037359534 1107 2 0.28967819428180164 1107 3 0.35260770667487407 1107 4 0.14622867029109443 1107 5 0.32432991579494796 1107 6 -0.013244012652535275 1107 7 0.03691438557191873 1107 8 0.09446326266375848 1107 9 0.21238514168863531 1107 10-11 0.18609167797577442 1107 12-13 0.18921805541602765 1107 14-15 0.3080499730540325 1107 16-17 0.24913256107781478 1107 18-19 0.23276284066030684 1107 20-21 0.4492461182077676 1107 22-23 0.4151802592238525 1107 24-25 0.28452601728880467 1107 26-27 0.4439623810617519 1107 28-29 0.40203904771347965 1107 30-31 0.4006772119092261 1107 32-33 0.388773472515787 1107 34-35 0.660951730448943 1107 36-37 0.6850881214682403 1107 38-39 0.7268124587778289 1107 40-41 0.8888128570377845 1107 42-43 0.873376445001945 1107 44-45 0.8387809046271784 1107 46-47 0.8468164781784182 1107 48-49 0.7995741828564888 1107 50-51 0.5170370635374582 1107 52-53 0.5576635852886582 1107 54-55 0.6051526284401163 1107 56-57 0.39315536587711364 1107 58-59 0.6895853986215243 1107 60-61 0.7838315171333861 1107 62-63 0.5056571947545265 1107 64-65 0.779583101684878 1107 66-67 0.905097980629229 1107 68-69 0.8587325918088524 1107 70-71 1.0545765874966868 1107 72-73 0.6176305559824975 1107 74-75 0.5922820537397726 1107 76-77 0.7965776971279688 1107 78-79 0.4437566639543995 1107 80-81 0.5794138537462068 1107 82-83 0.6955815517868125 1107 84-85 0.6510428436818678 1107 86-87 0.7885109207780303 1107 88-89 0.9814556393624088 1107 90-91 0.9454727810358357 1107 92-93 0.8519910643940136 1107 94-95 0.8467571639228915 1107 96-97 1.1051928534997764 1107 98-99 1.036996217682134 1107 100-101 1.3735945904404794 1108 1 0.21942548268553352 1108 2 0.18768380953981634 1108 3 0.09030617700114263 1108 4 -0.007525323319129029 1108 5 0.039464488357637606 1108 6 -0.10379004808675063 1108 7 -0.007420738835094198 1108 8 -0.02277945202305176 1108 9 -0.03598715959899579 1108 10-11 0.08425159181060593 1108 12-13 0.16388585965651714 1108 14-15 0.0397761808185777 1108 16-17 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-1.023593646996325 2316 64-65 -1.0094759672654874 2316 66-67 -1.2627443923761916 2316 68-69 -1.0155171520061117 2316 70-71 -1.2044585098536267 2316 72-73 -1.4883111070243835 2316 74-75 -1.1449452921832268 2316 76-77 -1.1720350608869623 2316 78-79 -0.9723884831690093 2316 80-81 -0.9000593200204428 2316 82-83 -0.7903145468810244 2316 84-85 -1.0313563292064352 2316 86-87 -0.8843865320107263 2316 88-89 -0.637761898333391 2316 90-91 -0.9440108231216833 2316 92-93 -0.8226905342006283 2316 94-95 -0.7915919064913979 2316 96-97 -0.3647374275718107 2316 98-99 -0.36364515986141654 2316 100-101 -0.09055322832690038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2664.0 3 489.0 4 427.0 5 459.0 6 628.0 7 695.0 8 871.0 9 1129.0 10 1370.0 11 1769.0 12 2557.0 13 3514.0 14 4011.0 15 5120.0 16 6820.0 17 9244.0 18 7702.0 19 4974.0 20 4666.0 21 4761.0 22 5218.0 23 5591.0 24 6306.0 25 6986.0 26 8211.0 27 9765.0 28 11407.0 29 13768.0 30 16836.0 31 21007.0 32 26892.0 33 35223.0 34 47694.0 35 67495.0 36 96796.0 37 128553.0 38 98737.0 39 12354.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.694247510702 19.248171772579965 14.543846608120298 21.51373410859774 2 14.952905967593665 20.4614539676759 41.69885077050947 22.886789294220957 3 19.658831268758846 25.862980388010786 29.66526287348828 24.81292546974209 4 12.1618803709991 16.576746180459693 37.908753957578284 33.35261949096292 5 12.89573622578002 38.76548213808437 33.63027317878363 14.708508457351979 6 30.802390674490766 36.67016970148859 17.74438805015015 14.783051573870493 7 27.90566273105513 34.18023584317236 20.166132216001234 17.747969209771274 8 26.407770124996517 35.24778427277624 19.353986547868367 18.99045905435888 9 27.043918075953233 17.07318505123922 20.595789900104798 35.287106972702745 10-11 24.798674292323533 27.660937638752 26.597230427574488 20.943157641349984 12-13 25.990477560329822 25.062157108512956 27.792813552157664 21.154551778999554 14-15 22.808382288727355 25.973646647855077 27.060951758908292 24.157019304509276 16-17 21.080731249513875 29.736692647147304 27.408942353270945 21.773633750067876 18-19 21.044300894735567 27.555991602268648 30.90341654992959 20.496290953066193 20-21 22.29991642777533 26.41696743618171 30.922163368657703 20.36095276738526 22-23 21.54748268430511 26.626655346677126 30.875100254553008 20.95076171446476 24-25 21.153720563167795 26.78745310175955 31.206252462776757 20.8525738722959 26-27 20.949642167117826 26.97362096938113 31.150209093570748 20.9265277699303 28-29 20.832079678164092 27.287144029201237 31.158480114949782 20.72229617768489 30-31 21.007192238458554 26.98802908009648 31.077646969037637 20.927131712407327 32-33 20.805073613481888 27.027471309749036 30.974389758838978 21.193065317930106 34-35 21.349442575600133 27.143691623076606 30.858606761889668 20.648259039433594 36-37 21.351484618172904 26.83223733932473 30.951423569276553 20.8648544732258 38-39 21.04195845753934 27.20935061199964 30.825808827791235 20.92288210266979 40-41 21.273156809661113 27.24840240940873 30.744979787085747 20.733460993844417 42-43 20.98009390622145 27.304272153408043 30.502572185179723 21.213061755190783 44-45 21.117434526237933 27.590773944920738 30.262555435870492 21.029236092970834 46-47 21.410075510933346 27.820396830463245 29.62265078523006 21.14687687337335 48-49 21.364217114064655 27.926176580019767 29.34098602949972 21.368620276415857 50-51 21.46074424133908 28.376934535821473 29.116993094743638 21.045328128095807 52-53 21.567109187843485 28.382542040490392 28.983057564939642 21.06729120672648 54-55 21.179889055208243 28.3317924334478 29.115743594259047 21.37257491708491 56-57 21.447133260748988 28.61097334432859 28.71236643551194 21.22952695941048 58-59 21.582309964474952 28.17667438014589 28.912340735240814 21.328674920138344 60-61 21.398640182404534 28.213940918067614 29.242093896079886 21.14532500344796 62-63 21.370502401629306 28.233285621111154 29.3091216426895 21.087090334570036 64-65 21.20594535903982 28.237740998327315 29.338996977433457 21.2173166651994 66-67 21.219566592177294 28.30928691222237 29.0248103895799 21.446336106020432 68-69 21.346581054868047 28.30383580376978 29.14144141235991 21.208141729002268 70-71 21.320638991887144 28.4222851868862 28.831889727385178 21.42518609384148 72-73 21.275133121356497 28.385699046691148 28.82134226238076 21.517825569571592 74-75 21.121260526584187 28.74237173471357 28.681470350197635 21.454897388504605 76-77 21.31240755523208 28.486488390111052 28.64114995421783 21.55995410043904 78-79 21.26819704943845 28.614225119907378 28.595295272600808 21.522282558053366 80-81 21.25216712485354 28.822412051231822 28.48697083818483 21.438449985729807 82-83 21.370222355240227 28.78353080638252 28.503606157168594 21.342640681208664 84-85 21.353047239138697 28.65142741451578 28.328005316768802 21.66752002957673 86-87 21.33345266189499 28.707811056916054 28.354960412505143 21.603775868683808 88-89 21.454986959918713 28.536026204877835 28.24397240124569 21.765014433957766 90-91 21.242821214869075 28.61235587405836 28.420725963358485 21.724096947714084 92-93 21.43828154517143 28.52118539929645 28.3775406522704 21.66299240326172 94-95 21.147546876639186 28.602324556030045 28.332498226467777 21.91763034086299 96-97 21.18897115573672 28.395635063871744 28.411335217351752 22.004058563039784 98-99 21.181914697701515 28.486853062605057 28.20400883090317 22.127223408790258 100-101 21.599106187217977 28.012051507208675 28.513390685831112 21.87545161974223 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 995.0 1 598.0 2 265.5 3 318.5 4 265.5 5 248.5 6 397.5 7 817.0 8 1313.5 9 1502.5 10 1383.0 11 1229.5 12 1253.0 13 1330.0 14 1385.5 15 1493.0 16 1513.0 17 1587.0 18 1715.0 19 1788.0 20 1940.0 21 2240.0 22 2647.0 23 3130.0 24 3667.0 25 4370.0 26 5403.5 27 6656.5 28 7539.5 29 8416.0 30 9794.5 31 11123.5 32 12521.5 33 13864.0 34 15180.0 35 16479.5 36 18061.0 37 19641.0 38 20483.5 39 20960.5 40 21471.0 41 22260.5 42 22856.0 43 23644.5 44 24647.5 45 25298.0 46 25265.5 47 25401.5 48 27375.0 49 26878.5 50 24169.5 51 22313.0 52 20356.0 53 19298.5 54 18600.5 55 17312.0 56 15436.5 57 13577.0 58 12314.0 59 10967.0 60 8977.0 61 7140.0 62 5748.0 63 4312.5 64 3090.0 65 2364.5 66 1744.5 67 1268.0 68 934.0 69 661.5 70 482.5 71 351.0 72 289.5 73 247.0 74 176.5 75 123.0 76 92.0 77 64.5 78 46.5 79 36.5 80 27.5 81 21.0 82 14.0 83 11.0 84 6.5 85 5.5 86 4.0 87 1.5 88 1.5 89 1.5 90 1.0 91 1.0 92 1.0 93 1.5 94 1.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2926563470394544 2 0.2539870399231301 3 0.24900690188542166 4 0.2548658878121375 5 0.25867422866450274 6 0.20477155813871736 7 0.19437185811879681 8 0.19525070600780417 9 0.20477155813871736 10-11 0.20733486448165553 12-13 0.20125616658268788 14-15 0.2042588968701297 16-17 0.1908564665627673 18-19 0.19590984192455968 20-21 0.18536366725647124 22-23 0.19356624755387336 24-25 0.19283387431303392 26-27 0.1931268236093697 28-29 0.20081674263818416 30-31 0.19810696164707814 32-33 0.20257443841619893 34-35 0.19979142010100892 36-37 0.1890987707847526 38-39 0.1781864094962444 40-41 0.17825964682032835 42-43 0.18858610951616495 44-45 0.18953819472925626 46-47 0.18858610951616495 48-49 0.20264767574028286 50-51 0.19730135108215469 52-53 0.21443888491779842 54-55 0.18924524543292046 56-57 0.1766484256904815 58-59 0.17884554541299993 60-61 0.16844584539307936 62-63 0.17511044188471858 64-65 0.17166828765277306 66-67 0.1732795087826199 68-69 0.17371893272712358 70-71 0.175916052449642 72-73 0.17445130596796307 74-75 0.18785373627532548 76-77 0.1792849693575036 78-79 0.18287359823761704 80-81 0.17891878273708386 82-83 0.1611221129846846 84-85 0.16038973974384513 86-87 0.16412484327212645 88-89 0.16983735455067436 90-91 0.17430483131979516 92-93 0.17130210103235333 94-95 0.17027677849517805 96-97 0.17430483131979516 98-99 0.18184827570044176 100-101 0.16661491229098066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 682712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.68775384230638 #Duplication Level Percentage of deduplicated Percentage of total 1 84.35773953343516 58.78701387299428 2 9.98803410063895 13.920873235477785 3 2.63092110011191 5.500289460093861 4 1.0408569088156456 2.9013992018643457 5 0.5449611508319053 1.898855926639691 6 0.32626171503538487 1.3641867651332766 7 0.2035083411461224 0.9927427427852961 8 0.15289456040510513 0.8523902791470886 9 0.11351792026998801 0.7119727996018936 >10 0.5894183589824689 7.247625427568639 >50 0.031427226805966824 1.4866138464677776 >100 0.01834989965405362 2.135240725590055 >500 0.0014764287071593607 0.6870045535000627 >1k 6.327551602111546E-4 1.5137911631358891 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4503 0.6595753407000317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3426 0.5018221446232086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2370 0.3471449161579114 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 916 0.1341707777217919 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.1318271833511056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.323732408394755E-5 7.323732408394755E-5 14-15 1.464746481678951E-4 0.0 0.0 1.464746481678951E-4 1.464746481678951E-4 16-17 2.1971197225184266E-4 0.0 0.0 3.6618662041973773E-4 1.464746481678951E-4 18-19 2.929492963357902E-4 0.0 0.0 5.858985926715804E-4 1.464746481678951E-4 20-21 2.929492963357902E-4 0.0 0.0 5.858985926715804E-4 1.464746481678951E-4 22-23 2.929492963357902E-4 0.0 0.0 0.0010253225371752658 1.464746481678951E-4 24-25 2.929492963357902E-4 0.0 0.0 0.0012450345094271085 1.464746481678951E-4 26-27 2.929492963357902E-4 0.0 0.0 0.0016844584539307938 1.464746481678951E-4 28-29 2.929492963357902E-4 0.0 0.0 0.002636543667022112 1.464746481678951E-4 30-31 2.929492963357902E-4 0.0 0.0 0.004540714093204748 2.1971197225184266E-4 32-33 2.929492963357902E-4 0.0 0.0 0.007030783112058965 2.929492963357902E-4 34-35 2.929492963357902E-4 0.0 0.0 0.009960276075416868 2.929492963357902E-4 36-37 2.929492963357902E-4 0.0 0.0 0.012523582418355031 2.929492963357902E-4 38-39 4.394239445036853E-4 0.0 0.0 0.015672787353964775 2.929492963357902E-4 40-41 4.394239445036853E-4 0.0 0.0 0.01977407750266584 2.929492963357902E-4 42-43 4.394239445036853E-4 0.0 0.0 0.028855505689075335 2.929492963357902E-4 44-45 4.394239445036853E-4 0.0 0.0 0.04218469867235379 2.929492963357902E-4 46-47 4.394239445036853E-4 0.0 0.0 0.055440654331548295 2.929492963357902E-4 48-49 4.394239445036853E-4 0.0 0.0 0.08642004241905811 2.929492963357902E-4 50-51 4.394239445036853E-4 0.0 0.0 0.11534878543221738 2.929492963357902E-4 52-53 4.394239445036853E-4 0.0 0.0 0.14530285098255194 3.6618662041973773E-4 54-55 4.394239445036853E-4 0.0 0.0 0.18646222711773047 4.394239445036853E-4 56-57 5.858985926715804E-4 0.0 0.0 0.23560447157805928 4.394239445036853E-4 58-59 5.858985926715804E-4 0.0 0.0 0.30181101254994785 4.394239445036853E-4 60-61 5.858985926715804E-4 0.0 0.0 0.3780510669213373 4.394239445036853E-4 62-63 5.858985926715804E-4 0.0 0.0 0.4840254748708094 4.394239445036853E-4 64-65 7.323732408394755E-4 0.0 0.0 0.5730088236328057 5.126612685876328E-4 66-67 7.323732408394755E-4 0.0 0.0 0.6904082541393736 5.858985926715804E-4 68-69 7.323732408394755E-4 0.0 0.0 0.8460375678177621 5.858985926715804E-4 70-71 0.0010253225371752658 0.0 0.0 0.9668059152321917 5.858985926715804E-4 72-73 0.0010253225371752658 0.0 0.0 1.1191395493268024 5.858985926715804E-4 74-75 0.0010253225371752658 0.0 0.0 1.3132916954733476 5.858985926715804E-4 76-77 0.0010253225371752658 0.0 0.0 1.4763179788842147 5.858985926715804E-4 78-79 0.0010253225371752658 0.0 0.0 1.6287248503029095 5.858985926715804E-4 80-81 0.0011717971853431608 7.323732408394755E-5 0.0 1.8582506239820011 5.858985926715804E-4 82-83 0.001464746481678951 1.464746481678951E-4 0.0 2.122490889276884 5.858985926715804E-4 84-85 0.001464746481678951 1.464746481678951E-4 0.0 2.398156177128863 5.858985926715804E-4 86-87 0.001464746481678951 1.464746481678951E-4 0.0 2.740101243276814 5.858985926715804E-4 88-89 0.001464746481678951 1.464746481678951E-4 0.0 3.141588253905014 5.858985926715804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3795 0.0 30.19418 1 CCGTCTA 65 0.0058396882 29.233568 9 GTATCAA 5450 0.0 28.615103 1 GTACATG 4385 0.0 27.432722 1 TACATGG 4420 0.0 26.781273 2 ACATGGG 4355 0.0 25.867266 3 AGGACCG 225 1.0186341E-10 25.35251 5 GTGTAAG 310 0.0 24.540096 1 GTGTAGC 480 0.0 23.773218 1 TATCAAC 6605 0.0 23.535807 2 ATCAACG 6640 0.0 23.40831 3 TCAACGC 6680 0.0 23.34101 4 CAACGCA 6720 0.0 23.131338 5 GAGTACT 3685 0.0 23.072094 12-13 CATGGGA 2840 0.0 22.931078 4 AACGCAG 6910 0.0 22.547539 6 AGCGTCG 75 3.0246363E-5 22.175303 26-27 GTACTTT 3875 0.0 21.572302 14-15 AGTACTT 3865 0.0 21.260237 12-13 GTAGCCC 315 1.4551915E-11 21.12554 3 >>END_MODULE