FastQCFastQC Report
Thu 26 May 2016
SRR937658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682712
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61790.9050668510294239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48640.7124526886886418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.515297812254655No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.18353273415437257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.13578199885163875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57200.030.824871
GGTATCA39200.030.673111
GTACGCT700.008373585527.1532423
CTCTACG1151.2776858E-424.7920882
TATCAAC72300.024.5149082
GTACATG40100.024.414411
ATCAACG73000.024.2798333
TCAACGC73500.024.1146644
TACATGG40300.023.9359532
CAACGCA74150.023.9032755
TAAGGTG4000.023.7590875
GTAAGGT3400.023.7590874
AACGCAG75650.023.4190226
ACATGGG40550.023.3196453
AGTACTT48150.022.9841512-13
GAGTACT46950.022.71169712-13
CACCGTT1050.00219604322.6177567
GTACTTT50150.021.30985314-15
ACGCAGA83900.021.172817
GGTAAGG3650.020.8298843
Produced by FastQC (version 0.11.5)