##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682712 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23557224715546 33.0 31.0 34.0 31.0 34.0 2 32.434067366620184 34.0 31.0 34.0 31.0 34.0 3 32.510219536202676 34.0 31.0 34.0 31.0 34.0 4 36.140622985973586 37.0 35.0 37.0 35.0 37.0 5 36.065009843096355 37.0 35.0 37.0 35.0 37.0 6 36.11436008155708 37.0 35.0 37.0 35.0 37.0 7 36.065824242140174 37.0 35.0 37.0 35.0 37.0 8 36.04720438486507 37.0 35.0 37.0 35.0 37.0 9 37.730923141822615 39.0 37.0 39.0 35.0 39.0 10-11 37.664376047293736 39.0 37.0 39.0 35.0 39.0 12-13 37.56415223403134 39.0 37.0 39.0 35.0 39.0 14-15 38.7416179882586 40.0 38.0 41.0 35.0 41.0 16-17 38.71677808504904 40.0 38.0 41.0 34.5 41.0 18-19 38.74952615451318 40.0 38.0 41.0 35.0 41.0 20-21 38.671932674392714 40.0 38.0 41.0 34.0 41.0 22-23 38.52700260138975 40.0 38.0 41.0 34.0 41.0 24-25 38.584449958401194 40.0 38.0 41.0 34.0 41.0 26-27 38.44351937566646 40.0 38.0 41.0 34.0 41.0 28-29 38.29103633743072 40.0 38.0 41.0 34.0 41.0 30-31 38.26318271833511 40.0 37.5 41.0 34.0 41.0 32-33 38.12192330001523 40.0 37.0 41.0 34.0 41.0 34-35 37.96501379791185 40.0 37.0 41.0 33.5 41.0 36-37 37.76348518848358 40.0 37.0 41.0 33.0 41.0 38-39 37.5933087451224 40.0 37.0 41.0 33.0 41.0 40-41 37.37646547885492 40.0 36.0 41.0 33.0 41.0 42-43 37.1019829737869 40.0 36.0 41.0 32.5 41.0 44-45 36.68664971466738 40.0 36.0 41.0 31.0 41.0 46-47 36.187653066007336 39.0 35.5 41.0 30.0 41.0 48-49 35.73685097083397 39.0 35.0 41.0 28.5 41.0 50-51 35.43975570958179 39.0 35.0 41.0 27.0 41.0 52-53 35.17776968912221 39.0 35.0 40.0 26.0 41.0 54-55 34.94550996613506 39.0 35.0 40.0 26.0 41.0 56-57 34.684941087896505 38.0 34.5 40.0 25.0 41.0 58-59 34.414437566645965 38.0 34.0 40.0 24.0 41.0 60-61 34.06040468015796 38.0 34.0 40.0 23.0 41.0 62-63 33.71283791701332 37.0 33.5 40.0 22.0 41.0 64-65 33.32279570301972 37.0 33.0 39.5 21.5 41.0 66-67 32.903594048442095 36.0 32.5 39.0 19.5 40.5 68-69 32.46088174808704 36.0 32.0 39.0 18.0 40.0 70-71 32.05051324716718 35.0 31.5 38.5 16.0 40.0 72-73 31.565883564372676 35.0 31.0 37.5 12.0 40.0 74-75 31.067330733896576 35.0 31.0 37.0 8.0 39.0 76-77 29.8370484186597 33.5 29.5 35.5 7.0 38.5 78-79 30.42766569212201 34.0 30.5 36.0 7.0 38.5 80-81 30.230319666272162 34.0 30.5 36.0 2.0 37.5 82-83 29.86296637527977 34.0 30.0 35.0 2.0 37.0 84-85 29.467727240769168 34.0 30.0 35.0 2.0 36.5 86-87 29.035183210489926 34.0 29.0 35.0 2.0 36.0 88-89 28.642040128194616 34.0 29.0 35.0 2.0 36.0 90-91 28.115258703523594 34.0 28.5 35.0 2.0 35.0 92-93 27.648882691383776 33.0 27.0 35.0 2.0 35.0 94-95 27.164509339223567 33.0 26.0 35.0 2.0 35.0 96-97 26.60592973318178 33.0 25.5 35.0 2.0 35.0 98-99 25.717149398282146 32.0 24.0 34.0 2.0 35.0 100-101 23.079735671849917 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19416501440966982 1101 2 0.17274470979531742 1101 3 0.16331227855929598 1101 4 0.1467329036442706 1101 5 0.05449596935803669 1101 6 -0.05020769945745229 1101 7 0.12488788274710316 1101 8 0.10759259569961444 1101 9 0.07268588040386703 1101 10-11 0.2158531632571723 1101 12-13 0.1781084476507644 1101 14-15 0.2133296524783006 1101 16-17 0.2711328420206698 1101 18-19 0.16716897910627893 1101 20-21 0.2872358765162417 1101 22-23 0.2653927680986925 1101 24-25 0.28390925255091304 1101 26-27 0.23062666659845377 1101 28-29 0.23437821158704963 1101 30-31 0.13675556305141612 1101 32-33 -0.003567560765709743 1101 34-35 0.06426749436148071 1101 36-37 0.16139113759844292 1101 38-39 0.1257941849310953 1101 40-41 0.051635698549546305 1101 42-43 -0.04985959322953448 1101 44-45 0.05600281960059306 1101 46-47 0.20952189026968426 1101 48-49 -0.11594995640103178 1101 50-51 0.05493748311523916 1101 52-53 0.014225577994800176 1101 54-55 -0.0037251424283013534 1101 56-57 0.20175788297452613 1101 58-59 -0.004893660046427328 1101 60-61 0.01014714955533691 1101 62-63 -0.2754807494385787 1101 64-65 -0.17013979440350369 1101 66-67 -0.302471476277546 1101 68-69 -0.23470552977426706 1101 70-71 -0.25886573073563923 1101 72-73 -0.41691202592715726 1101 74-75 -0.43831905778371905 1101 76-77 -0.46883812475379827 1101 78-79 -0.5104354402260967 1101 80-81 -0.3782776382120687 1101 82-83 -0.21679784736196694 1101 84-85 -0.3540536803818668 1101 86-87 -0.6187074095628127 1101 88-89 -0.37321695072150973 1101 90-91 -0.2329661074697249 1101 92-93 0.1493256640337357 1101 94-95 0.07159288118638685 1101 96-97 0.4183819320831539 1101 98-99 0.21619754812456904 1101 100-101 0.725751034844162 1102 1 0.32801164611147726 1102 2 0.43229790212895836 1102 3 0.3487988971800675 1102 4 0.15303396723096796 1102 5 0.184841097972253 1102 6 0.3076762925570975 1102 7 0.20141878121993528 1102 8 0.22324755739380464 1102 9 0.3424124511885509 1102 10-11 0.3214469478497435 1102 12-13 0.32240075132234836 1102 14-15 0.44608382616325315 1102 16-17 0.3769797638040231 1102 18-19 0.4644601375031172 1102 20-21 0.36628925288745506 1102 22-23 0.5282326389850454 1102 24-25 0.33196563783393174 1102 26-27 0.3471840281700338 1102 28-29 0.4190725792161345 1102 30-31 0.3978932573684162 1102 32-33 0.4338733453833328 1102 34-35 0.2765223446753069 1102 36-37 0.4560544582931598 1102 38-39 0.5110518854328561 1102 40-41 0.6400667816072811 1102 42-43 0.44375519217170734 1102 44-45 0.450774373548235 1102 46-47 0.5070415562927906 1102 48-49 0.513722648271191 1102 50-51 0.5907030315540638 1102 52-53 0.6340805645922103 1102 54-55 0.7414758553427276 1102 56-57 0.53654208009862 1102 58-59 0.6066262714286452 1102 60-61 0.7003653811799708 1102 62-63 0.6162285286552063 1102 64-65 0.7306878957948797 1102 66-67 0.7390717575021739 1102 68-69 0.9259640659861645 1102 70-71 0.8176672202009065 1102 72-73 0.6073330437129307 1102 74-75 0.674705329590072 1102 76-77 0.853612817942782 1102 78-79 0.9725085863519389 1102 80-81 0.7955927199702124 1102 82-83 0.857543072333339 1102 84-85 0.8171601056063764 1102 86-87 0.5761870185455393 1102 88-89 0.6104905923562818 1102 90-91 0.5139014908330246 1102 92-93 0.8591168737421633 1102 94-95 1.1708910754288162 1102 96-97 1.2385012760062857 1102 98-99 0.9436207894026296 1102 100-101 1.1182265660559771 1103 1 0.3510279501670581 1103 2 0.3009271139203591 1103 3 0.1895480126097695 1103 4 0.14669678731635827 1103 5 0.08900234250082661 1103 6 0.16295225698070226 1103 7 0.13843011662532945 1103 8 0.24208702267240056 1103 9 0.1771537479847396 1103 10-11 0.138225283674565 1103 12-13 0.2703919163740238 1103 14-15 0.32136748016452543 1103 16-17 0.25426512779528565 1103 18-19 0.28258078858987545 1103 20-21 0.314312009735481 1103 22-23 0.27002885442227864 1103 24-25 0.30850099913652684 1103 26-27 0.3185282523830608 1103 28-29 0.3199711305642339 1103 30-31 0.28407406461275286 1103 32-33 0.23911193738401693 1103 34-35 0.40099738312319744 1103 36-37 0.3172421216294694 1103 38-39 0.20111687043011273 1103 40-41 0.18951533671950216 1103 42-43 0.172614903086604 1103 44-45 0.16299327377731032 1103 46-47 0.12241278006487732 1103 48-49 0.447750911502105 1103 50-51 0.2884448230643457 1103 52-53 0.2043044227168167 1103 54-55 0.5046274529465862 1103 56-57 0.19374353090145036 1103 58-59 0.3130161491482326 1103 60-61 0.21360570467997064 1103 62-63 0.4319804671815959 1103 64-65 0.46108888307212936 1103 66-67 0.45222295180651884 1103 68-69 0.4716666406503549 1103 70-71 0.49030428826710803 1103 72-73 0.458631802625856 1103 74-75 0.3056217336639335 1103 76-77 0.39863717723426717 1103 78-79 0.43785709091729075 1103 80-81 0.570316325360448 1103 82-83 0.6178789670667832 1103 84-85 0.6930829893593184 1103 86-87 0.7580845159375365 1103 88-89 0.712840618705183 1103 90-91 0.9585358594810565 1103 92-93 0.7318577242202089 1103 94-95 0.923159635273084 1103 96-97 0.9684176316842077 1103 98-99 0.750825466601686 1103 100-101 0.8378345858015201 1104 1 0.23957332249145225 1104 2 0.19263347509009066 1104 3 0.12974548020096677 1104 4 0.16961810175711634 1104 5 0.21634783541610858 1104 6 0.08057209418299038 1104 7 0.17597759929039114 1104 8 0.10661219602181404 1104 9 0.3603403623953554 1104 10-11 0.2516952866893405 1104 12-13 0.2138807617161973 1104 14-15 0.2984732986752263 1104 16-17 0.3541400147413256 1104 18-19 0.28795989621724516 1104 20-21 0.2115203305072626 1104 22-23 0.19576521257724266 1104 24-25 0.045557827595985145 1104 26-27 0.16021049537371823 1104 28-29 0.2947947663797308 1104 30-31 0.4015808478506173 1104 32-33 0.2855575207424792 1104 34-35 0.33515852270716096 1104 36-37 0.19700772307655967 1104 38-39 0.24850984601046378 1104 40-41 0.36491328667428036 1104 42-43 0.48881402880261504 1104 44-45 0.42094624332811037 1104 46-47 0.5602157646895378 1104 48-49 0.3869881740887777 1104 50-51 0.5999929086838804 1104 52-53 0.5160153060451265 1104 54-55 0.6165855476104483 1104 56-57 0.5390291805849046 1104 58-59 0.5259766556299965 1104 60-61 0.33439708243094657 1104 62-63 0.47396192633143386 1104 64-65 0.51842834644264 1104 66-67 0.4692948541278881 1104 68-69 0.4779652538550607 1104 70-71 0.6174259343328323 1104 72-73 0.6057353600572704 1104 74-75 0.6313859784634523 1104 76-77 0.6047631947199434 1104 78-79 0.7858884445500927 1104 80-81 0.7240651948208026 1104 82-83 0.8967160186193901 1104 84-85 0.8141585094075552 1104 86-87 0.6751647088829671 1104 88-89 0.7491995273575824 1104 90-91 0.7435848495323114 1104 92-93 0.8621659846965422 1104 94-95 0.9392818842932833 1104 96-97 1.1999890817897239 1104 98-99 1.0168135582507674 1104 100-101 1.1828458911979673 1105 1 0.07627197225482973 1105 2 0.11630993929362887 1105 3 0.05475568295354094 1105 4 0.08258124159995361 1105 5 -0.03627752819262753 1105 6 0.017754397834274016 1105 7 0.07876577278059926 1105 8 0.027155763946097977 1105 9 0.1139061158794874 1105 10-11 0.11132625095799398 1105 12-13 0.2085727254204528 1105 14-15 0.09802437756204085 1105 16-17 0.0740488412076914 1105 18-19 0.0915147957092799 1105 20-21 0.18065331361845693 1105 22-23 -0.001669772010089332 1105 24-25 -0.052111603220666325 1105 26-27 -0.2185781991081086 1105 28-29 0.0510028157002651 1105 30-31 -0.0042273404010231275 1105 32-33 -0.051651700809209444 1105 34-35 -0.04215663380740864 1105 36-37 -0.3129944477218558 1105 38-39 -0.30961805743243076 1105 40-41 -0.25652465019890514 1105 42-43 -0.17345228474751195 1105 44-45 -0.13773564787147308 1105 46-47 -0.40879044379394003 1105 48-49 -0.3415882163076063 1105 50-51 -0.19880533731099348 1105 52-53 -0.23246209594332612 1105 54-55 -0.2295378405717301 1105 56-57 -0.27771201682750046 1105 58-59 -0.2783632045705886 1105 60-61 -0.3445618026469859 1105 62-63 -0.2305733057269137 1105 64-65 -0.2641740067028451 1105 66-67 -0.14749670580785335 1105 68-69 -0.1817803285701629 1105 70-71 -0.1963152196636102 1105 72-73 -0.2961063453219701 1105 74-75 -0.3427789153845069 1105 76-77 -0.2904862300184732 1105 78-79 -0.23873188280630941 1105 80-81 -0.2949353695867849 1105 82-83 -0.3767681128441609 1105 84-85 -0.28507215963251653 1105 86-87 -0.24928803283543388 1105 88-89 -0.3145985015574517 1105 90-91 -0.2825593783146907 1105 92-93 0.03784522752205177 1105 94-95 -0.004470992014621089 1105 96-97 -0.21942827591444214 1105 98-99 -0.5091830553037013 1105 100-101 -0.47608520766019424 1106 1 -0.07392106007279864 1106 2 -0.09796298568429762 1106 3 0.01894360932966066 1106 4 -0.17879599728333773 1106 5 -0.11625065052824368 1106 6 -0.03206785952740887 1106 7 0.016681586946496907 1106 8 -0.10843963868574491 1106 9 -0.11456006674780639 1106 10-11 -0.13802745789815418 1106 12-13 -0.31597024363603765 1106 14-15 -0.08782883739690561 1106 16-17 0.0033169278036666583 1106 18-19 -0.11691787030959233 1106 20-21 -0.08794830346737115 1106 22-23 -0.1613715828323734 1106 24-25 0.008503967718453964 1106 26-27 0.13103261794472587 1106 28-29 0.060883777421729235 1106 30-31 0.1380512659035631 1106 32-33 0.09212871511057585 1106 34-35 0.02650815383690741 1106 36-37 -0.06638684753918511 1106 38-39 0.12495615489709877 1106 40-41 0.29679814129600857 1106 42-43 0.07337258996756901 1106 44-45 0.24798901298913023 1106 46-47 0.14836080543403085 1106 48-49 0.21984414592927948 1106 50-51 0.42287443624065446 1106 52-53 0.5180433691147002 1106 54-55 0.4724812031386776 1106 56-57 0.33289580867690205 1106 58-59 0.32893822561890573 1106 60-61 0.44312074316675876 1106 62-63 0.35616550413171666 1106 64-65 0.32596776492955115 1106 66-67 0.29845826605710357 1106 68-69 0.3862125174646778 1106 70-71 0.4060271205038113 1106 72-73 0.45956341348318475 1106 74-75 0.499459656075679 1106 76-77 0.16782120137407475 1106 78-79 0.19260332954936743 1106 80-81 0.38575958702871915 1106 82-83 0.3163225570252912 1106 84-85 0.19073693652554624 1106 86-87 0.3923709386915384 1106 88-89 0.26351169284290066 1106 90-91 0.15370877064711763 1106 92-93 0.20564252647772818 1106 94-95 0.2944253338328018 1106 96-97 0.3226314464167537 1106 98-99 0.006796681834352114 1106 100-101 0.022434106637472695 1107 1 0.09656745738880801 1107 2 0.24033875368246527 1107 3 0.049892107766950744 1107 4 0.10149004701616349 1107 5 -0.022173669960231734 1107 6 -0.04421285205356895 1107 7 0.07042530212519438 1107 8 -0.03241028687163805 1107 9 -0.003938718738567104 1107 10-11 0.14251762295522497 1107 12-13 0.2410093052157123 1107 14-15 0.23058229183261147 1107 16-17 0.1619577078009513 1107 18-19 0.3245483615153901 1107 20-21 0.16590327283375217 1107 22-23 0.2013602761158495 1107 24-25 0.184058560733817 1107 26-27 0.18248322264644656 1107 28-29 0.15846412121843656 1107 30-31 0.20320108245471857 1107 32-33 0.19799398213054786 1107 34-35 0.2394107221206454 1107 36-37 0.19487062630236807 1107 38-39 0.19523149801436546 1107 40-41 0.30481553496363034 1107 42-43 0.22897287533242405 1107 44-45 0.3937883743153989 1107 46-47 0.47315442549110287 1107 48-49 0.766215935574607 1107 50-51 0.5533396828774286 1107 52-53 0.5765431652826649 1107 54-55 0.5939523609046731 1107 56-57 0.4980040095194056 1107 58-59 0.3598867240561887 1107 60-61 0.5028562710917512 1107 62-63 0.9517075275044533 1107 64-65 0.5587949153575948 1107 66-67 0.4637193409314051 1107 68-69 0.328629965487508 1107 70-71 0.5877094142937302 1107 72-73 0.7499179113769152 1107 74-75 0.39279604688964653 1107 76-77 0.4964494117287437 1107 78-79 0.8717462158110898 1107 80-81 0.8091739435989069 1107 82-83 0.8072817566448087 1107 84-85 1.1034925758787182 1107 86-87 1.0261654420207975 1107 88-89 1.1552259203194595 1107 90-91 1.424011291370043 1107 92-93 1.6235491714023098 1107 94-95 1.9510023144008102 1107 96-97 2.1428813105287254 1107 98-99 1.8851004595215386 1107 100-101 1.7617584034169305 1108 1 -0.03576626320369769 1108 2 0.049135587838726735 1108 3 0.00959295005565508 1108 4 0.08247804198179409 1108 5 -0.08933288188781319 1108 6 -0.05393063693109923 1108 7 0.0745883382371062 1108 8 -0.018856215809577748 1108 9 0.05497913390932041 1108 10-11 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2316 86-87 -0.2926156661624866 2316 88-89 -0.1369165185621135 2316 90-91 0.009261437761491464 2316 92-93 -0.02136006589823225 2316 94-95 -0.1307207129743908 2316 96-97 0.1027380298963152 2316 98-99 -0.2511808316510553 2316 100-101 0.5899405875773738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 9.0 5 18.0 6 35.0 7 65.0 8 157.0 9 338.0 10 599.0 11 1141.0 12 1862.0 13 2750.0 14 3494.0 15 5095.0 16 7290.0 17 10627.0 18 9338.0 19 5037.0 20 3914.0 21 3625.0 22 3868.0 23 4070.0 24 4601.0 25 5329.0 26 6340.0 27 7639.0 28 9444.0 29 12070.0 30 15056.0 31 18909.0 32 24332.0 33 32278.0 34 45413.0 35 66382.0 36 100197.0 37 147531.0 38 113966.0 39 9889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.845597112014914 19.55938888172623 15.538156799774963 21.0568572064839 2 15.387454739333716 20.874688008999403 40.92120835725753 22.816648894409354 3 19.746393794162106 25.618269489916685 30.44241202732631 24.192924688594896 4 12.464131967992314 17.021697321414187 37.6059562538175 32.908214456776 5 12.680808545488375 38.526339847544314 33.979187147191666 14.813664459775639 6 30.72730521801287 37.171750313455746 17.11922450462274 14.981719963908645 7 27.40013358487913 34.41978462367734 20.27297015432569 17.907111637117847 8 26.616054793236387 34.959690176824196 19.292029435545295 19.13222559439412 9 26.87824441345692 16.708802540456297 20.524906549174467 35.88804649691231 10-11 24.69291590011601 27.541261908388897 26.590202017834752 21.17562017366034 12-13 26.09836286494606 24.949372808748965 27.985539482617572 20.966724843687405 14-15 22.7799569950433 25.718238437291273 27.55839944222454 23.94340512544089 16-17 20.732021701683873 29.279915982141812 28.10775261017823 21.880309705996087 18-19 20.645674896588897 26.776664244952485 32.31355242034709 20.26410843811153 20-21 21.54664045746962 25.542761808786135 32.71672388942922 20.19387384431503 22-23 21.100548553181827 25.701510901487467 32.65400136222819 20.54393918310251 24-25 20.74366643621322 25.960800454657306 32.910143662334924 20.385389446794548 26-27 20.717758224953826 26.069159773783557 32.80402045380975 20.40906154745287 28-29 20.375960884951606 26.375998968806392 32.89456338325248 20.353476762989526 30-31 20.624143123308215 26.1031738126765 32.77311662897386 20.499566435041423 32-33 20.476643152603145 26.183295445224342 32.568418308159224 20.77164309401329 34-35 21.00805317615627 26.108739849306883 32.546886534878546 20.336320439658305 36-37 21.017354316314933 25.86083150728272 32.58204045043884 20.53977372596351 38-39 20.70288840893994 26.234214598699452 32.52354764787176 20.53934934448885 40-41 20.89162766137425 26.21243535838607 32.45699286589479 20.43894411434489 42-43 20.664608839476294 26.330030750208394 32.1215153304219 20.88384507989341 44-45 20.79611959077883 26.567298192574757 31.821567382415562 20.81501483423086 46-47 21.127576489061273 26.908491428303588 31.061047703863416 20.90288437877172 48-49 21.183456567337323 27.18972275278595 30.614300026951334 21.012520652925392 50-51 21.33161567395915 27.417124644066604 30.373349230715146 20.877910451259098 52-53 21.44293640656675 27.632515614197494 29.949378361593176 20.975169617642578 54-55 21.12157102848639 27.63163676630849 30.121779022486788 21.125013182718337 56-57 21.341356238062318 27.85435146884777 29.75654448728014 21.04774780580977 58-59 21.30078276051981 27.376697641172264 30.045612205439483 21.27690739286844 60-61 21.17657225887343 27.59274774721991 30.258952530496018 20.971727463410634 62-63 21.24548858083643 27.508891011143792 30.368295855353356 20.877324552666423 64-65 21.074772378396748 27.55005038727897 30.30172312776105 21.073454106563236 66-67 21.04144939593855 27.64555185788444 30.084940648472564 21.228058097704448 68-69 21.276556788628874 27.521215037391343 30.075368585412726 21.126859588567058 70-71 21.21547681560952 27.64469325622902 29.73642600131974 21.40340392684172 72-73 21.214289480776667 27.65983313608081 29.634531105356288 21.491346277786242 74-75 21.125965267931427 27.972483272595177 29.401343465472994 21.500207994000398 76-77 21.160679759547218 27.666351257924276 29.74592507528797 21.427043907240535 78-79 21.07689626079518 27.859624556181817 29.56722600452314 21.496253178499867 80-81 21.102853183850854 28.06363160986127 29.374288681447936 21.459226524839938 82-83 21.155722216525803 27.992573496023088 29.430927653839955 21.420776633611155 84-85 21.16205303751032 27.97062569438799 29.26371619973766 21.603605068364033 86-87 21.102016662955975 27.886136467500204 29.132489248760823 21.879357620782994 88-89 21.011129143767796 27.70538675166102 29.307526453321458 21.975957651249722 90-91 21.031675401298656 27.748735584194893 29.378174773210862 21.84141424129558 92-93 20.948966588056074 27.641608929382294 29.509294115923772 21.90013036663786 94-95 20.777502079940007 27.610251467675972 29.439207162024395 22.173039290359625 96-97 20.851911201209294 27.540309823175807 29.43408054933852 22.17369842627638 98-99 20.75133971631777 27.40879785498912 29.185125834828014 22.6547365938651 100-101 20.589844697133405 27.395452038217353 29.581822803392182 22.43288046125706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 391.0 1 278.5 2 250.5 3 344.5 4 282.0 5 246.5 6 435.5 7 955.0 8 1591.0 9 1869.0 10 1707.5 11 1454.5 12 1419.5 13 1546.0 14 1662.0 15 1749.0 16 1861.0 17 1958.0 18 2050.0 19 2165.0 20 2371.0 21 2633.5 22 2949.0 23 3420.5 24 3909.5 25 4667.0 26 5632.0 27 6743.0 28 7720.0 29 8584.5 30 9771.0 31 11113.5 32 12663.0 33 13774.0 34 14860.0 35 16254.5 36 17920.5 37 19457.0 38 20231.5 39 20637.0 40 21114.0 41 22216.5 42 22998.0 43 23624.0 44 24496.0 45 25053.0 46 25047.5 47 25269.5 48 27612.0 49 26994.5 50 23655.5 51 21944.5 52 20350.0 53 19288.5 54 18389.5 55 16958.5 56 15156.0 57 13489.0 58 12226.0 59 10646.5 60 8672.0 61 7085.5 62 5765.5 63 4288.0 64 2929.5 65 2090.5 66 1550.5 67 1146.0 68 869.5 69 606.5 70 423.5 71 319.5 72 243.5 73 211.5 74 173.5 75 138.5 76 111.5 77 76.0 78 50.0 79 29.0 80 24.0 81 17.5 82 8.5 83 5.5 84 4.0 85 4.0 86 1.5 87 0.5 88 1.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023435943706863218 2 0.0 3 0.0 4 1.464746481678951E-4 5 0.02241062116968795 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037351035282813254 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010253225371752658 24-25 0.0 26-27 4.3942394450368526E-4 28-29 0.0011717971853431608 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.323732408394755E-5 40-41 8.056105649234232E-4 42-43 0.016844584539307934 44-45 7.323732408394755E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.323732408394755E-5 70-71 7.323732408394755E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.464746481678951E-4 82-83 0.0032224422596936926 84-85 9.520852130913181E-4 86-87 0.0 88-89 0.0 90-91 0.012377107770187136 92-93 0.0032224422596936926 94-95 0.0 96-97 0.0 98-99 0.0016844584539307936 100-101 0.020872637363925053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 682712.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.09109019764722 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32452895264029 55.90333487638057 2 10.481418626176032 14.064196048961517 3 2.8572457969112453 5.750872064322623 4 1.1521188772778437 3.0918764605543947 5 0.5723258185750393 1.919898155823012 6 0.36103933028932245 1.4533513364003448 7 0.2422951619408887 1.1379092594960794 8 0.16746986649498263 0.8988588734722254 9 0.12674276887431674 0.7652979484601704 >10 0.6510181461333696 7.716500553832091 >50 0.0416543297898111 1.890639617829885 >100 0.0201692464409425 2.5917746952848812 >500 0.0010961546977476429 0.48949697436830913 >1k 6.576928186485857E-4 1.4171584579443086 >5k 2.1923093954952856E-4 0.9088346768695259 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6179 0.9050668510294239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4864 0.7124526886886418 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3518 0.515297812254655 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.18353273415437257 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.13578199885163875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.323732408394755E-5 0.0 14-15 1.464746481678951E-4 0.0 0.0 1.464746481678951E-4 0.0 16-17 1.464746481678951E-4 0.0 0.0 2.929492963357902E-4 0.0 18-19 2.1971197225184266E-4 0.0 0.0 2.929492963357902E-4 0.0 20-21 2.929492963357902E-4 0.0 0.0 2.929492963357902E-4 0.0 22-23 2.929492963357902E-4 0.0 0.0 7.323732408394755E-4 0.0 24-25 4.394239445036853E-4 0.0 0.0 9.520852130913182E-4 0.0 26-27 4.394239445036853E-4 0.0 0.0 0.001318271833511056 0.0 28-29 5.126612685876328E-4 0.0 0.0 0.0021971197225184266 0.0 30-31 5.858985926715804E-4 0.0 0.0 0.004028052824617115 0.0 32-33 5.858985926715804E-4 0.0 0.0 0.00659135916755528 1.464746481678951E-4 34-35 5.858985926715804E-4 0.0 0.0 0.009374377482745286 1.464746481678951E-4 36-37 5.858985926715804E-4 0.0 0.0 0.012084158473851345 1.464746481678951E-4 38-39 8.788478890073706E-4 0.0 0.0 0.015233363409461092 1.464746481678951E-4 40-41 8.788478890073706E-4 0.0 0.0 0.01948112820633005 1.464746481678951E-4 42-43 8.788478890073706E-4 0.0 0.0 0.028269607096403755 1.464746481678951E-4 44-45 8.788478890073706E-4 0.0 0.0 0.041672037403766155 1.464746481678951E-4 46-47 8.788478890073706E-4 0.0 0.0 0.05566036630380014 1.464746481678951E-4 48-49 8.788478890073706E-4 0.0 0.0 0.0867129917153939 1.464746481678951E-4 50-51 8.788478890073706E-4 0.0 0.0 0.11608115867305686 1.464746481678951E-4 52-53 8.788478890073706E-4 0.0 0.0 0.1474267333809864 1.464746481678951E-4 54-55 8.788478890073706E-4 0.0 0.0 0.18983114402559204 1.464746481678951E-4 56-57 0.0010253225371752658 0.0 0.0 0.23977899905084427 1.464746481678951E-4 58-59 0.0010253225371752658 0.0 0.0 0.30642496396723656 1.464746481678951E-4 60-61 0.0010253225371752658 0.0 0.0 0.38376357819988516 1.464746481678951E-4 62-63 0.0010253225371752658 0.0 0.0 0.48973798614935726 1.464746481678951E-4 64-65 0.0010253225371752658 0.0 0.0 0.5785016229391018 1.464746481678951E-4 66-67 0.0011717971853431608 0.0 0.0 0.6969263759828449 1.464746481678951E-4 68-69 0.001318271833511056 0.0 0.0 0.8528486389575692 1.464746481678951E-4 70-71 0.001611221129846846 0.0 0.0 0.9737634610201666 1.464746481678951E-4 72-73 0.0018309331020986887 0.0 0.0 1.1263168070870293 1.464746481678951E-4 74-75 0.0019774077502665837 0.0 0.0 1.3208351398539941 1.464746481678951E-4 76-77 0.0020506450743505316 0.0 0.0 1.4848867458020365 1.464746481678951E-4 78-79 0.0020506450743505316 0.0 0.0 1.6395639742673338 1.464746481678951E-4 80-81 0.0024900690188542165 0.0 0.0 1.871140393020776 2.1971197225184266E-4 82-83 0.0024900690188542165 0.0 0.0 2.1388228125476045 2.929492963357902E-4 84-85 0.002636543667022112 0.0 0.0 2.4177837799833606 2.929492963357902E-4 86-87 0.002783018315190007 0.0 0.0 2.764562509520852 2.929492963357902E-4 88-89 0.002783018315190007 0.0 0.0 3.1702240476218377 2.929492963357902E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5720 0.0 30.82487 1 GGTATCA 3920 0.0 30.67311 1 GTACGCT 70 0.0083735855 27.153242 3 CTCTACG 115 1.2776858E-4 24.792088 2 TATCAAC 7230 0.0 24.514908 2 GTACATG 4010 0.0 24.41441 1 ATCAACG 7300 0.0 24.279833 3 TCAACGC 7350 0.0 24.114664 4 TACATGG 4030 0.0 23.935953 2 CAACGCA 7415 0.0 23.903275 5 TAAGGTG 400 0.0 23.759087 5 GTAAGGT 340 0.0 23.759087 4 AACGCAG 7565 0.0 23.419022 6 ACATGGG 4055 0.0 23.319645 3 AGTACTT 4815 0.0 22.98415 12-13 GAGTACT 4695 0.0 22.711697 12-13 CACCGTT 105 0.002196043 22.617756 7 GTACTTT 5015 0.0 21.309853 14-15 ACGCAGA 8390 0.0 21.17281 7 GGTAAGG 365 0.0 20.829884 3 >>END_MODULE