FastQCFastQC Report
Thu 26 May 2016
SRR937657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642356
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45560.7092640218196764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.5279004165914228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.363038564285225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.14228869972414052No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8800.13699568463593398No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6630.10321379422002754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47850.032.297591
CCTATAC1401.695662E-830.5500433
GGTATCA32300.030.1800561
CCCTATA1004.928677E-528.5178282
GTACATG40650.027.607061
TACATGG41000.027.0107862
ATCAACG58700.026.230363
TATCAAC59600.025.9180472
TCAACGC59500.025.8776824
CAACGCA59800.025.749875
AACGCAG61300.025.2649756
CCGTTCA950.001225398725.0019729
ACATGGG41500.024.7344933
GCGTGCC1000.001652090923.7518759
CATGGGG12750.023.48164
AGTACTT34850.022.83000612-13
TCTATAC2303.59978E-922.728053
GCACCGC1050.00219316822.6225976
ACGCAGA67900.022.5992767
CGCAGAG68450.022.4176888
Produced by FastQC (version 0.11.5)