##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937657_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642356 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8672947711238 33.0 31.0 34.0 30.0 34.0 2 31.979779125593907 33.0 31.0 34.0 30.0 34.0 3 31.997177577542672 34.0 31.0 34.0 30.0 34.0 4 35.585491534289396 37.0 35.0 37.0 33.0 37.0 5 35.59368481029211 37.0 35.0 37.0 33.0 37.0 6 35.654764647640874 37.0 35.0 37.0 33.0 37.0 7 35.61024416367248 37.0 35.0 37.0 33.0 37.0 8 35.59860108724757 37.0 35.0 37.0 33.0 37.0 9 37.23591902309623 39.0 37.0 39.0 34.0 39.0 10-11 37.128246642048964 39.0 37.0 39.0 34.0 39.0 12-13 37.00613133527203 39.0 37.0 39.0 33.0 39.0 14-15 38.15149621082391 40.0 38.0 41.0 33.0 41.0 16-17 38.101627446462714 40.0 38.0 41.0 33.0 41.0 18-19 38.079755618379835 40.0 38.0 41.0 33.5 41.0 20-21 38.02388239543181 40.0 38.0 41.0 33.0 41.0 22-23 37.8333719931004 40.0 38.0 41.0 33.0 41.0 24-25 37.67951961217767 40.0 37.0 41.0 32.5 41.0 26-27 37.61331411242364 40.0 37.0 41.0 32.5 41.0 28-29 37.65360018432146 40.0 37.0 41.0 33.0 41.0 30-31 37.44937075391216 40.0 37.0 41.0 32.5 41.0 32-33 37.171189340490315 39.0 36.0 41.0 32.0 41.0 34-35 37.180357932361495 40.0 36.0 41.0 32.0 41.0 36-37 37.079795315992996 40.0 36.0 41.0 31.5 41.0 38-39 36.94459692133334 40.0 36.0 41.0 31.0 41.0 40-41 36.70843037194329 39.5 36.0 41.0 30.5 41.0 42-43 36.42362257066175 39.0 35.0 41.0 30.0 41.0 44-45 36.136864916027875 39.0 35.0 41.0 30.0 41.0 46-47 35.710272029840155 39.0 35.0 41.0 27.5 41.0 48-49 35.285845387915735 39.0 35.0 40.5 26.0 41.0 50-51 34.308265198737146 38.0 34.0 39.5 23.5 40.5 52-53 34.383822210736724 38.0 34.5 39.5 24.0 40.5 54-55 34.73036602756104 39.0 35.0 40.0 24.0 41.0 56-57 34.5404246243516 38.0 34.0 40.0 23.0 41.0 58-59 34.30005246312014 38.0 34.0 40.0 22.5 41.0 60-61 33.98446344394697 38.0 34.0 40.0 22.0 41.0 62-63 33.63606551507264 37.5 33.5 40.0 20.5 41.0 64-65 33.26900503770494 37.0 33.0 40.0 19.0 41.0 66-67 32.877283001949074 36.5 32.5 39.0 17.5 41.0 68-69 32.413741756907385 36.0 32.0 39.0 13.5 40.5 70-71 31.980146523111795 35.0 31.5 39.0 9.0 40.0 72-73 31.52941748812185 35.0 31.0 38.0 7.5 40.0 74-75 31.07828291477 35.0 31.0 37.0 4.5 39.5 76-77 30.606751552098835 35.0 30.5 37.0 2.0 39.0 78-79 30.08648553138758 34.5 30.0 36.0 2.0 39.0 80-81 29.570720908655012 34.0 29.0 36.0 2.0 37.5 82-83 29.054794381931515 34.0 29.0 35.0 2.0 37.0 84-85 28.61044420850743 34.0 29.0 35.0 2.0 36.5 86-87 28.14958605508472 34.0 27.5 35.0 2.0 36.0 88-89 27.679591223558276 33.5 26.5 35.0 2.0 36.0 90-91 27.185833089439498 33.0 26.0 35.0 2.0 35.0 92-93 26.639590040413726 33.0 25.0 35.0 2.0 35.0 94-95 26.02422644141255 32.5 24.5 35.0 2.0 35.0 96-97 25.322350067563782 32.0 20.5 34.0 2.0 35.0 98-99 24.42684819632727 32.0 9.5 34.0 2.0 35.0 100-101 22.08205418802035 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13829948438524298 1101 2 0.16953673331646613 1101 3 0.18242314702218465 1101 4 0.04850319268268777 1101 5 0.016208491530811386 1101 6 -0.002402297010249299 1101 7 0.12139711485673388 1101 8 0.06063491944080113 1101 9 0.09044997891582085 1101 10-11 0.15153644858087745 1101 12-13 0.3012894360730982 1101 14-15 0.39163081168626235 1101 16-17 0.42367704182692023 1101 18-19 0.32373174732954624 1101 20-21 0.4799898108806673 1101 22-23 0.5613519445388846 1101 24-25 0.39815437083020555 1101 26-27 0.33189836389068006 1101 28-29 0.3178421294939966 1101 30-31 0.5000337138720852 1101 32-33 0.6096303272269381 1101 34-35 0.7691304042996236 1101 36-37 0.6312582915714628 1101 38-39 0.583999459173782 1101 40-41 0.6905538699426828 1101 42-43 0.5817865155459145 1101 44-45 1.0179268363274545 1101 46-47 0.9285122789826374 1101 48-49 0.9716576881765135 1101 50-51 0.6593774633971492 1101 52-53 0.8429065224724752 1101 54-55 0.9217333874201543 1101 56-57 0.730294486002613 1101 58-59 0.9425947730728765 1101 60-61 1.0007991024509337 1101 62-63 0.983636591897671 1101 64-65 0.7896432643678679 1101 66-67 1.1554265223144355 1101 68-69 1.12281819831626 1101 70-71 1.2308004641795307 1101 72-73 1.2575924739926982 1101 74-75 1.1974791666514726 1101 76-77 1.2041059285965439 1101 78-79 1.352431586296678 1101 80-81 1.1283814617611263 1101 82-83 1.4588776080987031 1101 84-85 1.8019111202841742 1101 86-87 1.9400853254606965 1101 88-89 1.7392797947712246 1101 90-91 1.6728464340337403 1101 92-93 2.056682026585399 1101 94-95 1.9749934943541625 1101 96-97 2.018488506058098 1101 98-99 2.175568347712858 1101 100-101 2.301120744981141 1102 1 0.3006707868856502 1102 2 0.2033085914632231 1102 3 0.31617593887270345 1102 4 0.19240742540045375 1102 5 0.09735464831024387 1102 6 0.24410039950878826 1102 7 0.11444064048728819 1102 8 0.30584655532901905 1102 9 0.5163283904209663 1102 10-11 0.415868855836969 1102 12-13 0.39501934836813035 1102 14-15 0.36890787220813337 1102 16-17 0.3961357769143561 1102 18-19 0.45959000303582087 1102 20-21 0.3293987556973619 1102 22-23 0.3570865416952813 1102 24-25 0.5348106872217713 1102 26-27 0.49458153652238224 1102 28-29 0.5409041766694642 1102 30-31 0.6654569856485182 1102 32-33 0.4777746318518794 1102 34-35 0.6545637539754949 1102 36-37 0.7002905408496645 1102 38-39 0.7576089814268698 1102 40-41 0.8075460255236564 1102 42-43 0.973532171154126 1102 44-45 0.8449015598661518 1102 46-47 1.1541973037688251 1102 48-49 1.3128735731250316 1102 50-51 1.1576410685613965 1102 52-53 0.9615233988684011 1102 54-55 1.2019793426416001 1102 56-57 0.9844618336084281 1102 58-59 1.164896892155511 1102 60-61 1.1638704103988502 1102 62-63 1.2337945697807626 1102 64-65 1.2604050990903204 1102 66-67 1.4124578999905282 1102 68-69 1.2368264785363365 1102 70-71 1.1112915574954343 1102 72-73 1.2348981339589322 1102 74-75 1.4313722319671562 1102 76-77 1.5431087613034151 1102 78-79 1.4761963626762657 1102 80-81 1.6317112542108738 1102 82-83 1.321002410626349 1102 84-85 1.431546954026075 1102 86-87 1.7597236105835385 1102 88-89 1.935511477508058 1102 90-91 1.9998579282866125 1102 92-93 1.9775555353286585 1102 94-95 2.0277322316206003 1102 96-97 1.99815430112265 1102 98-99 2.007364120442606 1102 100-101 2.5078838326316273 1103 1 0.2972917361349907 1103 2 0.2231545655903382 1103 3 0.30610080078302815 1103 4 0.2098175409198575 1103 5 0.1101151438466701 1103 6 -0.06400326509555043 1103 7 0.02934278790792888 1103 8 0.12854806530175722 1103 9 0.13001332963266776 1103 10-11 0.013902430015996003 1103 12-13 0.17704389443888857 1103 14-15 0.42686587564054435 1103 16-17 0.4406475235504459 1103 18-19 0.28523430133835603 1103 20-21 0.19607263331313618 1103 22-23 0.4266599419462196 1103 24-25 0.347972111528442 1103 26-27 0.3902607358185861 1103 28-29 0.13624734573737385 1103 30-31 0.058538801077062885 1103 32-33 0.07052113814704342 1103 34-35 0.3029896037858464 1103 36-37 0.17118066040719526 1103 38-39 0.45127127825837476 1103 40-41 0.48346433989220117 1103 42-43 0.43423898838540964 1103 44-45 0.6488747928230794 1103 46-47 0.6390247457567426 1103 48-49 0.8733893002050266 1103 50-51 0.7111823656669216 1103 52-53 0.8040954001952159 1103 54-55 0.7155150152068188 1103 56-57 0.7552726327258128 1103 58-59 0.6068437041949082 1103 60-61 0.6616674654472376 1103 62-63 0.6631337584047685 1103 64-65 0.7433180340908194 1103 66-67 0.5576554078865144 1103 68-69 0.9848586105255563 1103 70-71 0.9596258282521504 1103 72-73 0.9320916325672179 1103 74-75 0.949246011514024 1103 76-77 0.7739435007718214 1103 78-79 0.9373055508676167 1103 80-81 0.7516004940326191 1103 82-83 0.9266276720232582 1103 84-85 0.8288240996439811 1103 86-87 0.7725093444418256 1103 88-89 0.7192810732622483 1103 90-91 0.7559706498430252 1103 92-93 0.8351291951737672 1103 94-95 0.9871309826842172 1103 96-97 1.3853433289424935 1103 98-99 1.780088705452485 1103 100-101 2.1158807751892112 1104 1 0.2003366336392638 1104 2 0.14419595397308527 1104 3 0.12605821173357867 1104 4 0.24956619460266438 1104 5 0.2528627623555124 1104 6 0.3344238784153646 1104 7 0.3288339821744515 1104 8 0.2702453115363781 1104 9 0.41222573111804905 1104 10-11 0.28595053774163404 1104 12-13 0.2009660385492893 1104 14-15 0.38974990744892324 1104 16-17 0.28492307909099424 1104 18-19 0.2236507384823554 1104 20-21 0.40754111116834224 1104 22-23 0.27909192527535964 1104 24-25 0.3965501405099374 1104 26-27 0.34900829353417606 1104 28-29 0.34608200498276176 1104 30-31 0.5042141212126268 1104 32-33 0.35350443182403524 1104 34-35 0.3775770118149424 1104 36-37 0.3613098629517353 1104 38-39 0.48375260544258225 1104 40-41 0.6723531912543947 1104 42-43 0.6881799952905112 1104 44-45 0.8194192808172573 1104 46-47 0.8371237745845548 1104 48-49 1.1482474070694337 1104 50-51 1.0967973316142547 1104 52-53 1.0311835217006617 1104 54-55 1.120397219840278 1104 56-57 1.1131920512721436 1104 58-59 0.7446349840785942 1104 60-61 0.9372534026174151 1104 62-63 1.0291960951284551 1104 64-65 0.7215025449837924 1104 66-67 0.6934042034202434 1104 68-69 0.9249764143355961 1104 70-71 1.0794518582333552 1104 72-73 0.932463146876426 1104 74-75 0.7782751264149574 1104 76-77 0.8746112133075599 1104 78-79 1.0225469760640529 1104 80-81 1.066620986424173 1104 82-83 1.0780459278986712 1104 84-85 1.1612339947587316 1104 86-87 1.064212247826152 1104 88-89 1.1224779538930143 1104 90-91 1.3070994622975363 1104 92-93 1.2574970585105412 1104 94-95 1.1863428657382649 1104 96-97 1.3201898513152521 1104 98-99 1.3793733715371097 1104 100-101 1.7972760140644652 1105 1 -0.11940103019202652 1105 2 -0.11187860819881479 1105 3 -0.14728008196833287 1105 4 -0.21628455326565188 1105 5 0.03328607004195305 1105 6 -0.05519727171685673 1105 7 0.048830107315893656 1105 8 -0.07040628302975449 1105 9 -0.24493791363986617 1105 10-11 -0.18693781892672945 1105 12-13 -0.01606829310974689 1105 14-15 -0.048181247856639686 1105 16-17 0.0049016849251231065 1105 18-19 0.10067447611321967 1105 20-21 0.021732986744169125 1105 22-23 -0.05325036488971335 1105 24-25 -0.15751650293506003 1105 26-27 -0.023064693961153182 1105 28-29 0.11238681207457546 1105 30-31 -0.14661990086993626 1105 32-33 -0.22041673953746965 1105 34-35 -0.04854303872310339 1105 36-37 -0.10517054943215243 1105 38-39 -0.12245561873859856 1105 40-41 -0.20808475020638895 1105 42-43 -0.007350932470167493 1105 44-45 -0.09603769485563873 1105 46-47 -0.24816227448187078 1105 48-49 -0.4386287767719992 1105 50-51 -0.6351191363284983 1105 52-53 -0.7350512081619271 1105 54-55 -0.5894263710406094 1105 56-57 -0.5451495092653076 1105 58-59 -0.5564889310125309 1105 60-61 -0.5258859748082401 1105 62-63 -0.43085576202117437 1105 64-65 -0.5722364668036093 1105 66-67 -0.4653712176099418 1105 68-69 -0.1598273385061404 1105 70-71 -0.08874276684316129 1105 72-73 -0.12242175127456534 1105 74-75 -0.10320469855685843 1105 76-77 0.14549474289148634 1105 78-79 0.3355572258893744 1105 80-81 0.3781864086703983 1105 82-83 0.3071943452868915 1105 84-85 0.24017908512744413 1105 86-87 0.7459049839091243 1105 88-89 0.7326053501735608 1105 90-91 0.5208556223951497 1105 92-93 0.9492458769878418 1105 94-95 1.3442598407103894 1105 96-97 1.1810185351618365 1105 98-99 1.047137227763372 1105 100-101 1.6315588251506838 1106 1 -0.06230264443147604 1106 2 0.1262028820228558 1106 3 0.127858622121245 1106 4 0.09150295960056809 1106 5 0.018873397132551872 1106 6 -0.020625434295624245 1106 7 0.08610071209082548 1106 8 -0.22954066909156268 1106 9 0.06974840172679109 1106 10-11 -0.06522215603414594 1106 12-13 -0.03789943814025065 1106 14-15 -0.07325342419284908 1106 16-17 -0.23017852495110702 1106 18-19 0.05033807162447346 1106 20-21 -0.02471844799588041 1106 22-23 0.09233244333462665 1106 24-25 -0.16007342809761838 1106 26-27 0.004342229946153964 1106 28-29 -0.10277398425007789 1106 30-31 -0.1290879549959172 1106 32-33 -0.006058041975137485 1106 34-35 -0.23405505137592542 1106 36-37 -0.1564835311307391 1106 38-39 -0.2592178510585299 1106 40-41 -0.17837822546560034 1106 42-43 -0.2016044408677331 1106 44-45 -0.29881428712837277 1106 46-47 -0.4888873014695463 1106 48-49 0.02949313219795613 1106 50-51 -0.4568586487930446 1106 52-53 -0.500159284380139 1106 54-55 -0.21486633288129298 1106 56-57 -0.4100831070360016 1106 58-59 -0.32810291941411407 1106 60-61 -0.3817391618641963 1106 62-63 0.028330088906450612 1106 64-65 0.11658561538953904 1106 66-67 0.0438598219803481 1106 68-69 0.015138142376549979 1106 70-71 -0.16386389756392106 1106 72-73 0.020209986229513532 1106 74-75 -0.048230179941644025 1106 76-77 -0.012930432214581344 1106 78-79 0.1752542108013806 1106 80-81 -0.05862226755283828 1106 82-83 -0.10085551287091477 1106 84-85 0.21002820520376275 1106 86-87 0.4330889770806188 1106 88-89 0.34994524423125384 1106 90-91 0.6138300405911181 1106 92-93 0.32809256189733915 1106 94-95 0.4373798668193949 1106 96-97 0.5680242841639185 1106 98-99 0.41087834693591674 1106 100-101 0.945456849401932 1107 1 0.2065556798595587 1107 2 0.11354855198385749 1107 3 0.28232649507939556 1107 4 0.37807148067156504 1107 5 0.1339882098836611 1107 6 0.07511637631466073 1107 7 0.11760310219024461 1107 8 0.2711602312280945 1107 9 0.1656837664002424 1107 10-11 0.3100117217921863 1107 12-13 0.27126798612439273 1107 14-15 0.5257406812061376 1107 16-17 0.5070234457811722 1107 18-19 0.3863054020447194 1107 20-21 0.5393714080116325 1107 22-23 0.5550292966147836 1107 24-25 0.47804059284141687 1107 26-27 0.47901597404422347 1107 28-29 0.19686823399679554 1107 30-31 0.1386603660692174 1107 32-33 0.3066593397662558 1107 34-35 0.3693644037400574 1107 36-37 0.27570949505771125 1107 38-39 0.4616669256211381 1107 40-41 0.646883512415279 1107 42-43 0.4120235247617572 1107 44-45 0.5050685246842832 1107 46-47 0.6828743337690355 1107 48-49 0.5440067542983087 1107 50-51 0.7475183124081681 1107 52-53 0.7440311610874701 1107 54-55 0.8224500107064898 1107 56-57 0.7015829242259102 1107 58-59 0.7876951978327256 1107 60-61 1.1483819560571504 1107 62-63 0.9585113258928644 1107 64-65 0.6838295228355094 1107 66-67 0.6424961456262039 1107 68-69 0.8625764836768823 1107 70-71 1.0610077995532947 1107 72-73 1.307686158551288 1107 74-75 1.2287310212666078 1107 76-77 1.4197552770437554 1107 78-79 1.4486214649730584 1107 80-81 1.3672611894400397 1107 82-83 1.5394579860586717 1107 84-85 1.8262192958515833 1107 86-87 1.793545295447725 1107 88-89 1.5733828816986275 1107 90-91 1.8423455395793589 1107 92-93 1.9362687547475694 1107 94-95 1.9615374667490109 1107 96-97 2.1336918990499854 1107 98-99 2.2180369146358494 1107 100-101 2.1928981233395852 1108 1 0.17198010429398636 1108 2 0.18614426859672406 1108 3 0.22883245977426014 1108 4 0.13528291502993994 1108 5 0.1519106105525907 1108 6 0.3538929892474343 1108 7 0.34514763335956644 1108 8 0.32652978913110786 1108 9 0.39098966313656547 1108 10-11 0.28582647479284873 1108 12-13 0.40655212946361985 1108 14-15 0.47779825941676535 1108 16-17 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-1.1672494718484145 2316 60-61 -1.412156274751034 2316 62-63 -1.5704767870031517 2316 64-65 -1.6661298746288544 2316 66-67 -1.4649492507054838 2316 68-69 -1.4228743243285606 2316 70-71 -1.5811909700849966 2316 72-73 -1.5370997866787413 2316 74-75 -1.5891643590338234 2316 76-77 -1.6172649463151387 2316 78-79 -1.6754844552062522 2316 80-81 -1.761519266207312 2316 82-83 -1.783938962907989 2316 84-85 -1.645643596427341 2316 86-87 -1.8523106988948896 2316 88-89 -1.6821688782939397 2316 90-91 -1.459900634642974 2316 92-93 -1.6037359797178397 2316 94-95 -1.469538605322736 2316 96-97 -1.2121443184118021 2316 98-99 -1.491587534383246 2316 100-101 -1.46420072810308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2506.0 3 436.0 4 416.0 5 453.0 6 550.0 7 609.0 8 792.0 9 955.0 10 1204.0 11 1621.0 12 2281.0 13 3126.0 14 3626.0 15 4617.0 16 6429.0 17 8633.0 18 7476.0 19 4578.0 20 4295.0 21 4370.0 22 4691.0 23 5067.0 24 5516.0 25 6381.0 26 7280.0 27 8725.0 28 10213.0 29 12363.0 30 15194.0 31 18790.0 32 24235.0 33 32033.0 34 43752.0 35 62354.0 36 90709.0 37 124196.0 38 99100.0 39 12779.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77833750396118 19.117034113810526 14.657246199212913 21.44738218301537 2 14.998962090552526 20.40608012036628 41.77967913770795 22.81527865137324 3 19.578906955947694 25.893585908006262 29.57655038306014 24.950956752985903 4 12.092082594691767 16.550304611620184 37.97595565653791 33.381657137150135 5 12.87308862059226 38.76953902186685 33.63934129484593 14.718031062694962 6 30.687189374865454 36.67396723480063 17.844117270835476 14.794726119498444 7 27.801573535651002 34.226428161421545 20.192043724575107 17.779954578352342 8 26.294508100181734 35.19542614677826 19.431856294898093 19.078209458141913 9 26.972523435342787 17.126169427115265 20.640885839577297 35.26042129796465 10-11 24.791012259626072 27.60845638850776 26.567437303677888 21.03309404818828 12-13 26.073021464329337 25.093094254910557 27.74442799488937 21.08945628587073 14-15 22.882329587600157 25.928002988890718 27.074966402278793 24.114701021230328 16-17 21.056527422882407 29.59765004664339 27.515233201359052 21.83058932911515 18-19 20.984157910787925 27.535330955447 31.09685661938193 20.383654514383146 20-21 22.161425695873696 26.35004293278076 31.108289653160842 20.380241718184706 22-23 21.55420211645988 26.562819243096552 30.955916183821635 20.927062456621933 24-25 21.07982743723076 26.69318639087401 31.299149351641876 20.927836820253354 26-27 20.981001872593453 26.964409805495176 31.205549303178415 20.849039018732952 28-29 20.806690388572303 27.184383477448332 31.34349080165169 20.665435332327675 30-31 21.00311670322059 26.93967198180519 31.159076657172008 20.89813465780221 32-33 20.70175028079371 27.00424310495445 31.15499812804193 21.13900848620991 34-35 21.311151927809043 27.080111219002383 31.03860297701916 20.570133876169418 36-37 21.261697002120453 26.817612162152678 31.02233299773137 20.898357837995498 38-39 20.959690293679767 27.180878567077947 30.956750142523244 20.90268099671904 40-41 21.12501306232327 27.252451427653448 30.856560873545792 20.765974636477495 42-43 20.96173779168119 27.219288414488148 30.603605268843122 21.21536852498754 44-45 21.007214554824298 27.59210430884627 30.433005866943674 20.96767526938575 46-47 21.295818980274472 27.7763176673449 29.763532299159213 21.164331053221417 48-49 21.30416436907387 27.85454619194955 29.55100812002443 21.290281318952157 50-51 21.503043917430084 28.250361721129586 29.26606685308467 20.980527508355664 52-53 21.513594298943534 28.30450356004019 29.094514230800435 21.08738791021585 54-55 21.185845166593744 28.246337714115917 29.253363968394723 21.314453150895616 56-57 21.401124093146276 28.56136974679833 28.808005888701054 21.22950027135434 58-59 21.498889997029075 28.13847784165829 29.02094384952305 21.341688311789582 60-61 21.396414487100405 28.142665638327824 29.347584508475606 21.113335366096166 62-63 21.346562080330166 28.22115362119012 29.376771289397297 21.055513009082418 64-65 21.195110140368634 28.233036418178596 29.37081695465331 21.201036486799456 66-67 21.163393167685395 28.31746883378382 29.147762099572805 21.371375898957982 68-69 21.24373383743383 28.322274940652775 29.205776567437482 21.22821465447592 70-71 21.23698091313746 28.404458072223765 29.008116361549284 21.35044465308949 72-73 21.27661731425782 28.375269623860095 28.825851449925217 21.52226161195687 74-75 21.046433916106547 28.780636925190883 28.752016856986224 21.420912301716346 76-77 21.171590996832716 28.413838715740724 28.811211609059946 21.603358678366614 78-79 21.202541765430336 28.518268061564573 28.790711496208175 21.488478676796912 80-81 21.25888207690274 28.714796325426978 28.60069707389623 21.425624523774054 82-83 21.31042572340847 28.713985559127558 28.525168578233874 21.4504201392301 84-85 21.26265796872015 28.55996475070089 28.4855668937335 21.69181038684546 86-87 21.25661317894264 28.590457058008813 28.42707722446128 21.725852538587276 88-89 21.329068907751772 28.497640060020153 28.380812892478453 21.792478139749623 90-91 21.12736580389759 28.49446432303771 28.571049766500185 21.80712010656451 92-93 21.353703617051146 28.310717015269915 28.695818320777377 21.639761046901565 94-95 21.041352451153948 28.49494733657024 28.454940910586807 22.008759301689004 96-97 21.067509262024647 28.46465224616282 28.526334094154105 21.941504397658427 98-99 21.115985857171154 28.433199776035483 28.262886927553094 22.18792743924027 100-101 21.428576998506703 27.98537112200219 28.678137391365375 21.907914488125733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 998.0 1 607.0 2 256.5 3 304.0 4 257.0 5 237.5 6 392.5 7 764.5 8 1274.0 9 1529.0 10 1364.0 11 1197.0 12 1222.0 13 1278.0 14 1353.0 15 1445.0 16 1501.0 17 1540.0 18 1648.5 19 1755.0 20 1879.0 21 2130.0 22 2437.5 23 2873.0 24 3457.0 25 4143.5 26 5118.5 27 6332.0 28 7184.0 29 8005.5 30 9139.5 31 10351.0 32 11860.5 33 13279.0 34 14358.0 35 15340.0 36 16904.0 37 18395.5 38 19236.0 39 19771.5 40 20144.0 41 20903.5 42 21493.0 43 22178.0 44 23241.5 45 23927.5 46 23883.0 47 23974.5 48 25810.0 49 25379.5 50 22564.0 51 20736.5 52 19048.5 53 18032.0 54 17370.0 55 16239.5 56 14534.0 57 12803.5 58 11589.0 59 10175.0 60 8349.0 61 6765.0 62 5461.0 63 4037.0 64 2780.0 65 2084.5 66 1552.5 67 1164.5 68 896.5 69 646.0 70 464.0 71 350.0 72 269.5 73 212.0 74 177.5 75 140.0 76 99.5 77 70.0 78 47.5 79 34.0 80 27.0 81 22.0 82 13.0 83 7.0 84 8.5 85 7.0 86 5.0 87 4.0 88 3.5 89 4.0 90 3.0 91 1.5 92 0.5 93 0.5 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27476975384366303 2 0.25608852412058114 3 0.24830467840263032 4 0.23974244811288442 5 0.23709594056878117 6 0.2039367578103108 7 0.19490749677748787 8 0.20564920386825997 9 0.20814003449800422 10-11 0.21008599592749191 12-13 0.20845138832672225 14-15 0.2047929808392854 16-17 0.205804880782619 18-19 0.2040145962674903 20-21 0.19265018151928215 22-23 0.1854112050015879 24-25 0.18650094340210102 26-27 0.1971648120356936 28-29 0.2050264962108239 30-31 0.20144592718056653 32-33 0.2048708192964649 34-35 0.1990329350080018 36-37 0.18984799706081987 38-39 0.19187179694748707 40-41 0.18665662031646002 42-43 0.1971648120356936 44-45 0.19015935088953786 46-47 0.19187179694748707 48-49 0.20027835032287392 50-51 0.2060383961541575 52-53 0.21047518821338948 54-55 0.1962307505495395 56-57 0.17560355939696992 58-59 0.1777051977408166 60-61 0.18564472037312643 62-63 0.19101557391851248 64-65 0.1792619668844068 66-67 0.186345266487742 68-69 0.18899177403184528 70-71 0.18401011277235677 72-73 0.18447714351543382 74-75 0.1864231049449215 76-77 0.1977875196931297 78-79 0.20323621169569522 80-81 0.1942069506628723 82-83 0.195997235178001 84-85 0.1881355510028707 86-87 0.18868042020312722 88-89 0.19272801997646166 90-91 0.1933507276338977 92-93 0.19085989700415346 94-95 0.1882133894600502 96-97 0.18097441294235597 98-99 0.1841657896867158 100-101 0.1803517052849199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 642356.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.10394010288307 #Duplication Level Percentage of deduplicated Percentage of total 1 84.72629350280317 59.39647004859804 2 9.835934630562878 13.790755443937067 3 2.4738422298923 5.202782625250574 4 1.005867553781188 2.8206111496683968 5 0.5397356278598261 1.8918797063438617 6 0.31102158758004605 1.3082303247849087 7 0.19250653730331105 0.9446826732367319 8 0.16346560477036998 0.9167666372562858 9 0.11181315543123675 0.7054688475959198 >10 0.5876612908175957 7.175034878192549 >50 0.0333852321052247 1.6064733934601432 >100 0.016692512433465326 2.076734262591301 >500 0.0011128341621511031 0.5602969749546957 >1k 6.67700497290662E-4 1.6038130341295789 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4556 0.7092640218196764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3391 0.5279004165914228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2332 0.363038564285225 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.14228869972414052 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 880 0.13699568463593398 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 663 0.10321379422002754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.5567691435901588E-4 0.0 0.0 0.0 0.0 6 1.5567691435901588E-4 0.0 0.0 0.0 0.0 7 3.1135382871803175E-4 0.0 0.0 0.0 0.0 8 3.1135382871803175E-4 0.0 0.0 0.0 0.0 9 3.1135382871803175E-4 0.0 0.0 0.0 0.0 10-11 3.1135382871803175E-4 0.0 0.0 0.0 0.0 12-13 3.891922858975397E-4 0.0 0.0 0.0 0.0 14-15 6.227076574360635E-4 0.0 0.0 0.0 0.0 16-17 6.227076574360635E-4 0.0 0.0 0.0 0.0 18-19 7.783845717950794E-4 0.0 0.0 0.0 0.0 20-21 7.783845717950794E-4 0.0 0.0 7.783845717950794E-5 0.0 22-23 9.340614861540952E-4 0.0 0.0 3.891922858975397E-4 7.783845717950794E-5 24-25 0.0010897384005131112 0.0 0.0 9.340614861540952E-4 1.5567691435901588E-4 26-27 0.0010897384005131112 0.0 0.0 0.0014010922292311428 1.5567691435901588E-4 28-29 0.001245415314872127 0.0 0.0 0.0021016383438467144 1.5567691435901588E-4 30-31 0.0014010922292311428 0.0 0.0 0.004358953602052445 1.5567691435901588E-4 32-33 0.0014010922292311428 0.0 0.0 0.007005461146155714 1.5567691435901588E-4 34-35 0.0014789306864106508 0.0 0.0 0.009963322518977016 1.5567691435901588E-4 36-37 0.0015567691435901588 0.0 0.0 0.011909283948464714 1.5567691435901588E-4 38-39 0.0018681229723081904 0.0 0.0 0.014477953035388475 1.5567691435901588E-4 40-41 0.0018681229723081904 0.0 0.0 0.018603391265902398 1.5567691435901588E-4 42-43 0.0018681229723081904 0.0 0.0 0.025219660126160573 1.5567691435901588E-4 44-45 0.0020237998866672064 0.0 0.0 0.037907328646420366 1.5567691435901588E-4 46-47 0.0021016383438467144 0.0 0.0 0.05051715870950065 1.5567691435901588E-4 48-49 0.0021794768010262224 0.0 0.0 0.0785390032941235 1.5567691435901588E-4 50-51 0.0021794768010262224 0.0 0.0 0.11021925536618324 1.5567691435901588E-4 52-53 0.0021794768010262224 0.0 0.0 0.13816326149362657 2.335153715385238E-4 54-55 0.0021794768010262224 0.0 0.0 0.18066305911363792 3.1135382871803175E-4 56-57 0.002335153715385238 0.0 0.0 0.230323994794164 3.1135382871803175E-4 58-59 0.002335153715385238 0.0 0.0 0.2933731451095654 3.1135382871803175E-4 60-61 0.002335153715385238 0.0 0.0 0.3691878024024061 3.1135382871803175E-4 62-63 0.002335153715385238 0.0 0.0 0.47489242725217795 4.670307430770476E-4 64-65 0.002335153715385238 0.0 0.0 0.5660412606093816 4.670307430770476E-4 66-67 0.002335153715385238 0.0 0.0 0.687469253809414 4.670307430770476E-4 68-69 0.002490830629744254 0.0 0.0 0.8520975907440733 4.670307430770476E-4 70-71 0.002568669086923762 0.0 0.0 0.9708790764000024 4.670307430770476E-4 72-73 0.00264650754410327 0.0 0.0 1.1306814289895324 4.670307430770476E-4 74-75 0.00264650754410327 0.0 0.0 1.3406895864598447 4.670307430770476E-4 76-77 0.00264650754410327 0.0 0.0 1.5183169457434817 4.670307430770476E-4 78-79 0.00264650754410327 0.0 0.0 1.6831009595925002 4.670307430770476E-4 80-81 0.0028021844584622856 0.0 0.0 1.9139698235869207 4.670307430770476E-4 82-83 0.0028021844584622856 0.0 0.0 2.2017385997795613 6.227076574360635E-4 84-85 0.0029578613728213016 0.0 0.0 2.4839808455124572 6.227076574360635E-4 86-87 0.0031135382871803176 0.0 0.0 2.842816133109989 6.227076574360635E-4 88-89 0.0031135382871803176 0.0 0.0 3.2612756789070234 6.227076574360635E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4785 0.0 32.29759 1 CCTATAC 140 1.695662E-8 30.550043 3 GGTATCA 3230 0.0 30.180056 1 CCCTATA 100 4.928677E-5 28.517828 2 GTACATG 4065 0.0 27.60706 1 TACATGG 4100 0.0 27.010786 2 ATCAACG 5870 0.0 26.23036 3 TATCAAC 5960 0.0 25.918047 2 TCAACGC 5950 0.0 25.877682 4 CAACGCA 5980 0.0 25.74987 5 AACGCAG 6130 0.0 25.264975 6 CCGTTCA 95 0.0012253987 25.001972 9 ACATGGG 4150 0.0 24.734493 3 GCGTGCC 100 0.0016520909 23.751875 9 CATGGGG 1275 0.0 23.4816 4 AGTACTT 3485 0.0 22.830006 12-13 TCTATAC 230 3.59978E-9 22.72805 3 GCACCGC 105 0.002193168 22.622597 6 ACGCAGA 6790 0.0 22.599276 7 CGCAGAG 6845 0.0 22.417688 8 >>END_MODULE