FastQCFastQC Report
Thu 26 May 2016
SRR937657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642356
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.8968547036222905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47480.7391539893766074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.5176257402437278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.19366208146261576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.14275573046721757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36050.036.5142251
GTATCAA53950.032.414851
GTACACG753.0688965E-431.6808131
CCGACGA300.003951519331.66602174-75
ATACCGT953.4889592E-529.9993866
ATCAACG67600.025.508123
TATCAAC68550.025.1546152
TCAACGC68400.025.140334
CAACGCA69450.024.8286385
GGACCGA1151.281071E-424.7821036
AACGCAG71400.024.2151896
GTACATG39800.023.6412121
GAGTACT47350.023.37515312-13
TACATGG40450.022.8999522
GTACTTT49450.022.0925714-15
ACGCAGA79200.021.832067
GTATAGG1754.029009E-621.7239861
CGCAGAG79450.021.6437848
ACATGGG40100.021.5598413
AGGACCG1553.901244E-521.4528435
Produced by FastQC (version 0.11.5)