##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937657_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642356 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27538312088624 33.0 31.0 34.0 31.0 34.0 2 32.474126496833534 34.0 31.0 34.0 31.0 34.0 3 32.52603229361912 34.0 31.0 34.0 31.0 34.0 4 36.126710110904234 37.0 35.0 37.0 35.0 37.0 5 36.060679747678854 37.0 35.0 37.0 35.0 37.0 6 36.12147002596691 37.0 35.0 37.0 35.0 37.0 7 36.07641245664398 37.0 35.0 37.0 35.0 37.0 8 36.05144032281165 37.0 35.0 37.0 35.0 37.0 9 37.73776223776224 39.0 37.0 39.0 35.0 39.0 10-11 37.669358579977455 39.0 37.0 39.0 35.0 39.0 12-13 37.55559300450218 39.0 37.0 39.0 35.0 39.0 14-15 38.755419891773414 40.0 38.0 41.0 35.0 41.0 16-17 38.725579740829076 40.0 38.0 41.0 34.5 41.0 18-19 38.75679841085006 40.0 38.0 41.0 35.0 41.0 20-21 38.67929855095928 40.0 38.0 41.0 34.0 41.0 22-23 38.62107772014272 40.0 38.0 41.0 34.0 41.0 24-25 38.62243600122051 40.0 38.0 41.0 34.0 41.0 26-27 38.436928276532015 40.0 38.0 41.0 34.0 41.0 28-29 38.376956080428926 40.0 38.0 41.0 34.0 41.0 30-31 38.32050996643606 40.0 38.0 41.0 34.0 41.0 32-33 38.12185532632995 40.0 37.0 41.0 34.0 41.0 34-35 37.93170298090156 40.0 37.0 41.0 33.0 41.0 36-37 37.72952459383893 40.0 37.0 41.0 33.0 41.0 38-39 37.550290648799105 40.0 37.0 41.0 33.0 41.0 40-41 37.32967466638438 40.0 36.0 41.0 33.0 41.0 42-43 37.040710291489454 40.0 36.0 41.0 32.0 41.0 44-45 36.6230213464185 40.0 36.0 41.0 31.0 41.0 46-47 36.1106520371881 39.5 35.5 41.0 29.5 41.0 48-49 35.63123252526637 39.0 35.0 41.0 27.5 41.0 50-51 35.31685155894862 39.0 35.0 41.0 26.5 41.0 52-53 35.035535590856156 39.0 35.0 40.0 25.5 41.0 54-55 34.797163877974214 39.0 35.0 40.0 24.0 41.0 56-57 34.53073918512476 38.0 34.0 40.0 23.5 41.0 58-59 34.26325044056567 38.0 34.0 40.0 23.0 41.0 60-61 33.90890487517825 38.0 34.0 40.0 22.0 41.0 62-63 33.50896932542079 37.0 33.5 40.0 20.5 41.0 64-65 33.12280262035382 37.0 33.0 39.5 18.5 41.0 66-67 32.70770569590694 36.0 32.5 39.0 17.0 40.5 68-69 32.247041360242605 36.0 32.0 39.0 12.5 40.0 70-71 31.824081973236026 35.0 31.5 38.5 8.0 40.0 72-73 31.362757567454807 35.0 31.0 37.5 7.0 40.0 74-75 30.88386657865732 35.0 31.0 37.0 6.0 39.0 76-77 29.643326286358345 33.5 29.5 35.5 2.0 38.0 78-79 30.231560069494172 34.0 30.0 36.0 2.0 38.5 80-81 30.057574771621965 34.0 30.0 36.0 2.0 37.5 82-83 29.662604537047994 34.0 30.0 35.0 2.0 37.0 84-85 29.25770133695334 34.0 29.5 35.0 2.0 36.5 86-87 28.91844864841303 34.0 29.0 35.0 2.0 36.0 88-89 28.5112624463693 34.0 29.0 35.0 2.0 36.0 90-91 28.012598932679076 34.0 28.5 35.0 2.0 35.0 92-93 27.566021022610514 33.0 27.0 35.0 2.0 35.0 94-95 27.071380978771895 33.0 26.0 35.0 2.0 35.0 96-97 26.572951758837778 33.0 25.0 35.0 2.0 35.0 98-99 25.786370797501696 32.5 24.0 34.5 2.0 35.0 100-101 23.381164805808616 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.333177631022437 1101 2 0.29483548007607396 1101 3 0.31071422737582566 1101 4 0.2532313521490792 1101 5 0.2521823148117548 1101 6 0.28393156082169924 1101 7 0.1827299434352767 1101 8 0.30455936254872995 1101 9 0.2544135984104443 1101 10-11 0.3169243583549033 1101 12-13 0.28941018060478285 1101 14-15 0.5047559465809783 1101 16-17 0.5137414727360081 1101 18-19 0.48899537255231706 1101 20-21 0.40450398588615144 1101 22-23 0.40389254963098153 1101 24-25 0.3197786000702365 1101 26-27 0.3723357989005365 1101 28-29 0.5274378514099354 1101 30-31 0.7443616916741789 1101 32-33 0.5665502876788935 1101 34-35 0.5441092172047775 1101 36-37 0.5359418694986715 1101 38-39 0.6205992488311409 1101 40-41 0.590617564044102 1101 42-43 0.7419491265614795 1101 44-45 0.559905730453309 1101 46-47 0.5416870050261906 1101 48-49 0.6134739178941331 1101 50-51 0.7513076549233446 1101 52-53 0.8417643254553795 1101 54-55 0.7510394603535033 1101 56-57 0.8209794661060812 1101 58-59 0.874580207415562 1101 60-61 1.0640483038964206 1101 62-63 0.8779938606898341 1101 64-65 1.0442321829515109 1101 66-67 0.8304625005491815 1101 68-69 0.9866552585928616 1101 70-71 1.0301488493667286 1101 72-73 0.9140444709235815 1101 74-75 1.0604442141720192 1101 76-77 1.0091007272713703 1101 78-79 0.9098953598120687 1101 80-81 1.0044620302042304 1101 82-83 0.9523987247608936 1101 84-85 1.0800754265345596 1101 86-87 1.2411480840472642 1101 88-89 1.1631096107358232 1101 90-91 1.0442354053355487 1101 92-93 1.4248927592475553 1101 94-95 1.5025353155404666 1101 96-97 1.2382117484314286 1101 98-99 1.6168662840074148 1101 100-101 2.128281509257537 1102 1 0.19918787234727375 1102 2 0.16048618552903093 1102 3 0.19924448831170594 1102 4 0.13769232071692272 1102 5 -0.021903864847232057 1102 6 0.0968802997223932 1102 7 0.1276555848799532 1102 8 0.22509648735125865 1102 9 0.29846491398487984 1102 10-11 0.2959446231040417 1102 12-13 0.35200755490340896 1102 14-15 0.22748978108553075 1102 16-17 0.28566226589393295 1102 18-19 0.2766717357924975 1102 20-21 0.35274307724094456 1102 22-23 0.25203630950460365 1102 24-25 0.28088744138416644 1102 26-27 0.33891258159923154 1102 28-29 0.36144803825963123 1102 30-31 0.295095744079255 1102 32-33 0.40542374194701836 1102 34-35 0.4315840224561853 1102 36-37 0.4392335293559526 1102 38-39 0.5432227711931787 1102 40-41 0.6747530436976348 1102 42-43 0.44998012291164713 1102 44-45 0.6008042977573353 1102 46-47 0.7083250722485062 1102 48-49 0.7277858969756821 1102 50-51 0.6197992385154336 1102 52-53 0.9541179235378507 1102 54-55 0.8982585048596903 1102 56-57 1.2354466993141173 1102 58-59 1.241884233127827 1102 60-61 1.0015517085921815 1102 62-63 0.982335528718373 1102 64-65 1.1203938651435834 1102 66-67 1.0681048168317986 1102 68-69 1.188013051260512 1102 70-71 1.1774618634757879 1102 72-73 1.220104829369948 1102 74-75 1.1341027640439982 1102 76-77 1.1204533448014722 1102 78-79 0.9592321250787279 1102 80-81 1.1386465374766601 1102 82-83 1.0001434542918624 1102 84-85 1.0954782701364572 1102 86-87 1.2658947746829874 1102 88-89 1.2770606920671952 1102 90-91 1.1365721161270699 1102 92-93 1.420741754181364 1102 94-95 1.4236158297857138 1102 96-97 1.5020960977259783 1102 98-99 1.6495540870253365 1102 100-101 2.0723355485885797 1103 1 0.22193553239428354 1103 2 0.254855876467758 1103 3 0.1173930050194727 1103 4 0.12300217519997148 1103 5 0.17373351094416734 1103 6 0.18430440448305774 1103 7 0.24382397496346186 1103 8 0.15487482277576703 1103 9 0.19871152844427797 1103 10-11 0.23002933733412334 1103 12-13 0.23173685587400428 1103 14-15 0.3697985827700023 1103 16-17 0.19054191515336782 1103 18-19 0.25115218690947216 1103 20-21 0.34348205530523757 1103 22-23 0.4168209184548317 1103 24-25 0.2296695120803136 1103 26-27 0.16116773382236715 1103 28-29 0.3486579778318841 1103 30-31 0.4491915631709631 1103 32-33 0.40198916688824937 1103 34-35 0.3180043631014158 1103 36-37 0.4755666175170177 1103 38-39 0.5205811120980073 1103 40-41 0.6728104140087154 1103 42-43 0.6643558710476185 1103 44-45 0.4907356495218522 1103 46-47 0.6768896607196311 1103 48-49 0.7718217209708342 1103 50-51 0.821975502955361 1103 52-53 0.56007633078341 1103 54-55 0.7133120822115373 1103 56-57 0.6520737057607136 1103 58-59 0.7284040848707107 1103 60-61 0.6682364026316137 1103 62-63 0.5018945070711069 1103 64-65 0.606134934383121 1103 66-67 0.6597408369647653 1103 68-69 0.7684562361925131 1103 70-71 0.8043680778717253 1103 72-73 0.7618966060035923 1103 74-75 0.7408853827722766 1103 76-77 0.6549260080934118 1103 78-79 0.7337839645918436 1103 80-81 0.8970011431098541 1103 82-83 0.8047642304784226 1103 84-85 0.9264067519432295 1103 86-87 0.9967994603577175 1103 88-89 1.1380898387234772 1103 90-91 1.3512449553661696 1103 92-93 1.4059146006314158 1103 94-95 1.148178485722486 1103 96-97 1.533056307708815 1103 98-99 1.708285825296727 1103 100-101 2.1935083373510373 1104 1 0.17517370177441194 1104 2 0.07458869013137814 1104 3 0.03547072243556215 1104 4 0.03785785033434763 1104 5 0.15118596977784904 1104 6 0.13698023629515177 1104 7 0.1275163543606297 1104 8 0.15865413652129234 1104 9 0.09271005251925146 1104 10-11 0.16639062486763123 1104 12-13 0.10533671132117206 1104 14-15 0.14914863537305223 1104 16-17 0.14718281786559118 1104 18-19 0.1623248042784411 1104 20-21 0.049199660524003264 1104 22-23 0.1481780771133927 1104 24-25 0.017825298162378544 1104 26-27 0.060678179284920475 1104 28-29 0.005309519362960202 1104 30-31 0.13529236851265836 1104 32-33 0.15054912676470877 1104 34-35 -0.06842371257267388 1104 36-37 0.04133900420937664 1104 38-39 0.12875594673192126 1104 40-41 0.2699588728153728 1104 42-43 0.22651359238149382 1104 44-45 0.027461527688799947 1104 46-47 0.053024256976463846 1104 48-49 0.19013332353157608 1104 50-51 0.08030882221617475 1104 52-53 0.11283220704212482 1104 54-55 0.059967018032445196 1104 56-57 0.09767810043724978 1104 58-59 -0.038816869887291716 1104 60-61 0.07140288663707395 1104 62-63 0.18038011342467541 1104 64-65 0.3071824358649522 1104 66-67 0.4351249743169845 1104 68-69 0.12656056754274658 1104 70-71 0.30293099297563586 1104 72-73 0.3534520857552188 1104 74-75 0.6428866628079426 1104 76-77 0.5184932884904576 1104 78-79 0.6552509057287779 1104 80-81 0.5619838930249941 1104 82-83 0.30878502852493384 1104 84-85 0.4154648111862542 1104 86-87 0.5726010446973753 1104 88-89 0.6420473104672837 1104 90-91 0.6281919858691865 1104 92-93 0.7853725612542775 1104 94-95 0.9376556806543555 1104 96-97 1.173840810833756 1104 98-99 1.3793014609830436 1104 100-101 1.5375356537849747 1105 1 -0.009791022585048381 1105 2 -0.0103346062190397 1105 3 -0.09493269568434926 1105 4 -0.1404474761533976 1105 5 -0.043735904417033566 1105 6 -0.05831675718560092 1105 7 0.019560321638685707 1105 8 0.034642173926037856 1105 9 0.06799546264623046 1105 10-11 0.10457323376042638 1105 12-13 -0.031316977317459305 1105 14-15 -0.10265842964830796 1105 16-17 0.04775147396983215 1105 18-19 0.20011085849760235 1105 20-21 0.10141234903690588 1105 22-23 0.20124508949438535 1105 24-25 0.12058221880403153 1105 26-27 -0.08835238469250584 1105 28-29 -0.07189497629740771 1105 30-31 0.031469861767732255 1105 32-33 -0.13224237905067326 1105 34-35 0.07301789969826444 1105 36-37 -0.04283508411678838 1105 38-39 -0.08406859843594106 1105 40-41 0.055378274726841425 1105 42-43 0.17709987084778334 1105 44-45 0.2754720557619521 1105 46-47 0.09482944640139834 1105 48-49 0.05612195865387548 1105 50-51 -0.1729028790322289 1105 52-53 -0.3120109951574719 1105 54-55 -0.16723057295974542 1105 56-57 -0.37565854438867774 1105 58-59 -0.1821938503419247 1105 60-61 -0.12481026356109481 1105 62-63 -0.39426759391632515 1105 64-65 -0.21850705193232756 1105 66-67 -0.16803695148771425 1105 68-69 -0.1408994832838566 1105 70-71 -0.15149819707586687 1105 72-73 -0.22855476120230023 1105 74-75 -0.08326183084079375 1105 76-77 -0.032392054857048436 1105 78-79 -0.1880131267211027 1105 80-81 -0.3275228663359826 1105 82-83 -0.31499093869541284 1105 84-85 -0.3283348998482296 1105 86-87 -0.2295585706198615 1105 88-89 -0.3149355762611208 1105 90-91 0.08640225465657991 1105 92-93 0.08661808094906931 1105 94-95 0.17658708535241274 1105 96-97 0.3170395065728542 1105 98-99 0.4464261716568352 1105 100-101 0.5701792760357307 1106 1 -0.5780685813930724 1106 2 -0.5249104604102932 1106 3 -0.6371414170962453 1106 4 -0.2653414083201966 1106 5 -0.6296295102212639 1106 6 -0.5790006122806233 1106 7 -0.5513155852726754 1106 8 -0.5557778295886351 1106 9 -0.8126713211762251 1106 10-11 -0.7736144498185169 1106 12-13 -1.0731102140809412 1106 14-15 -0.9837783553380675 1106 16-17 -0.8528811673075012 1106 18-19 -0.7364241752683682 1106 20-21 -0.7368273015041069 1106 22-23 -0.8026084992385663 1106 24-25 -0.8924873466316541 1106 26-27 -0.8125760183630746 1106 28-29 -1.0828896289723247 1106 30-31 -1.2544049154270596 1106 32-33 -1.4348909367791904 1106 34-35 -1.6008542665960164 1106 36-37 -1.6587691086693042 1106 38-39 -1.7763551447668746 1106 40-41 -2.1057444124923066 1106 42-43 -2.2914651380644244 1106 44-45 -2.8011365351049164 1106 46-47 -2.500568841450381 1106 48-49 -2.994006075890347 1106 50-51 -3.0794941475784086 1106 52-53 -3.147135138455738 1106 54-55 -3.371460733881598 1106 56-57 -3.5024278060561436 1106 58-59 -3.45409277836643 1106 60-61 -3.5919380219523838 1106 62-63 -3.758063943676575 1106 64-65 -4.311253016333708 1106 66-67 -3.8756675592752288 1106 68-69 -3.7709373915508735 1106 70-71 -3.962410003869227 1106 72-73 -3.998992589133916 1106 74-75 -3.858812253856886 1106 76-77 -3.654655002664139 1106 78-79 -3.499624102545166 1106 80-81 -3.486791563145161 1106 82-83 -3.6973410002022042 1106 84-85 -3.7815709101914123 1106 86-87 -3.5535512400145812 1106 88-89 -3.7626886955340844 1106 90-91 -4.218053849578602 1106 92-93 -4.134119749493024 1106 94-95 -4.332809646000985 1106 96-97 -4.349025063005133 1106 98-99 -4.536323056370179 1106 100-101 -4.2896580447786725 1107 1 0.08581012472734528 1107 2 0.13024951495555825 1107 3 0.02620354903179134 1107 4 0.10178665995159974 1107 5 0.11393188454104575 1107 6 -0.033230941615798315 1107 7 0.08225431413787021 1107 8 0.034696964393596375 1107 9 0.004824793079322376 1107 10-11 0.02322856610570767 1107 12-13 0.10270872489087424 1107 14-15 0.1402414106374792 1107 16-17 0.199838853807492 1107 18-19 0.4402232337465577 1107 20-21 0.31025032540577513 1107 22-23 0.22281746633226618 1107 24-25 0.25768714528892644 1107 26-27 0.43238717703854945 1107 28-29 0.22987174232888918 1107 30-31 0.19870954540757424 1107 32-33 0.0967434219996548 1107 34-35 0.1674835145705842 1107 36-37 0.5141538509482899 1107 38-39 0.43083714826224195 1107 40-41 0.11924752275506734 1107 42-43 0.227583807652465 1107 44-45 0.6252146775406118 1107 46-47 0.6672953187889235 1107 48-49 0.5874552572865852 1107 50-51 0.43580688764256337 1107 52-53 0.7157787299861482 1107 54-55 0.7666606186507394 1107 56-57 0.6436263304287948 1107 58-59 0.8073562377337495 1107 60-61 0.5340862016318866 1107 62-63 0.6592298186038406 1107 64-65 0.8521110119369197 1107 66-67 1.0466512770542806 1107 68-69 0.6608905212911438 1107 70-71 0.7278052517101123 1107 72-73 0.8046688085610363 1107 74-75 0.8176591969187967 1107 76-77 0.5526619274511084 1107 78-79 0.7530871206578595 1107 80-81 0.805752206466849 1107 82-83 0.6600287173848223 1107 84-85 0.6003977692420044 1107 86-87 0.7682265247292541 1107 88-89 0.7954083161084817 1107 90-91 0.834768813842615 1107 92-93 1.2985161049308545 1107 94-95 1.3599789988273088 1107 96-97 1.2086173898527228 1107 98-99 1.212439370680471 1107 100-101 1.7973884961756106 1108 1 0.13215518757233724 1108 2 0.06946326123699009 1108 3 -0.14606470066922128 1108 4 0.010022636006205232 1108 5 -0.0662048986878574 1108 6 0.1143656911049149 1108 7 0.17870547892734834 1108 8 0.06625104973487339 1108 9 0.11355513423788466 1108 10-11 0.13958425580165823 1108 12-13 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-0.21045793749540564 2316 60-61 0.02851121878683216 2316 62-63 0.016379310847405293 2316 64-65 -0.14165777235424315 2316 66-67 0.04844617881412461 2316 68-69 0.016433891091850228 2316 70-71 0.10210519507631588 2316 72-73 0.2899425235881665 2316 74-75 0.029811191237438806 2316 76-77 -0.04421482362687712 2316 78-79 -0.03527778077236832 2316 80-81 0.07308020004236226 2316 82-83 0.1130181727115449 2316 84-85 0.18048664534583736 2316 86-87 0.4852328443416276 2316 88-89 0.656734925302338 2316 90-91 0.29396251640431714 2316 92-93 0.3458442782961946 2316 94-95 0.299469234741526 2316 96-97 0.14523997804712607 2316 98-99 -0.0819020442055276 2316 100-101 0.6984546498419739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 20.0 6 38.0 7 76.0 8 212.0 9 391.0 10 649.0 11 1207.0 12 1891.0 13 2692.0 14 3684.0 15 5256.0 16 7384.0 17 10654.0 18 9278.0 19 5219.0 20 3919.0 21 3779.0 22 3845.0 23 3974.0 24 4366.0 25 5009.0 26 5815.0 27 6896.0 28 8775.0 29 10956.0 30 13676.0 31 17088.0 32 21992.0 33 29124.0 34 40089.0 35 59448.0 36 91950.0 37 141158.0 38 112306.0 39 9529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74082097653129 19.658022485979355 15.493490777758224 21.107665759731127 2 15.48487131746259 20.789562174246058 40.93711275367553 22.78845375461582 3 19.794631014577586 25.63578451824222 30.424095050096835 24.14548941708336 4 12.47952848576179 17.194203837124586 37.547714974251036 32.778552702862584 5 12.72181518696463 38.43478423325663 34.03319871589285 14.810201863885888 6 30.598141840350213 37.1923045787694 17.13473525583944 15.074818325040942 7 27.36488800602781 34.535211004489724 20.25434494267976 17.845556046802706 8 26.678975521361988 35.00940288562728 19.206172278300507 19.105449314710224 9 26.7818779617533 16.69043334225881 20.639178274975247 35.88851042101265 10-11 24.786333435042252 27.606498577113005 26.49800110841963 21.109166879425118 12-13 26.19265947923559 25.05507336628654 27.939205230996773 20.813061923481104 14-15 22.763856802147096 25.83668557622253 27.589140601161972 23.8103170204684 16-17 20.74893049959835 29.30267639751166 28.140548231821604 21.80784487106838 18-19 20.577608055346257 26.793164538044323 32.30856409841272 20.3206633081967 20-21 21.522850700041797 25.610468985583505 32.78423362800447 20.08244668637022 22-23 21.13749373386763 25.706399394714964 32.65332175071847 20.502785120698945 24-25 20.771815006009128 25.89444171144973 32.94536051659827 20.388382765942872 26-27 20.62201776304011 26.126458523067818 32.81569872887623 20.43582498501584 28-29 20.30529003331569 26.450400099635708 32.965641249182674 20.27866861786593 30-31 20.603077267961435 26.1259536191408 32.801462741425894 20.469506371471873 32-33 20.446917285741865 26.126945183044914 32.65953770183512 20.7665998293781 34-35 20.8684125313689 26.1676546961498 32.626300680619465 20.337632091861835 36-37 21.038555064652826 25.932351943009618 32.58514775341945 20.443945238918104 38-39 20.704484740548853 26.258414337221105 32.54947412338329 20.487626798846744 40-41 20.810675749938117 26.35373385039067 32.48719940593412 20.34839099373709 42-43 20.648181451568952 26.384639458896398 32.13324240201917 20.83393668751547 44-45 20.84264800204248 26.58658127268991 31.808685526405917 20.76208519886169 46-47 21.085971019185624 26.9641756284677 31.049838407362895 20.900014944983777 48-49 21.18474802134642 27.160095025188525 30.605925686068165 21.049231267396895 50-51 21.283369346592856 27.569992340695816 30.35419611554963 20.792442197161698 52-53 21.525324781468193 27.63238396213932 29.829299997664844 21.012991258727652 54-55 21.091575388102548 27.67087098120046 30.09382647628418 21.143727154412815 56-57 21.364866211259802 27.799382274003825 29.785274832024612 21.050476682711768 58-59 21.29029696928183 27.443894040065008 29.971542260055173 21.294266730597986 60-61 21.18264638300257 27.64207075204404 30.2768246891132 20.898458175840187 62-63 21.30306247625927 27.620353822490955 30.25962239007653 20.816961311173245 64-65 21.082623965526903 27.605953707912747 30.296362141865256 21.01506018469509 66-67 21.012880707894066 27.749721338323297 30.002911158298513 21.234486795484127 68-69 21.26258647852593 27.558550087490424 30.075612277304174 21.103251156679473 70-71 21.254491278979255 27.706754509960206 29.790490008655635 21.248264202404897 72-73 21.201483289640013 27.697180379727133 29.69085678346587 21.41047954716699 74-75 21.198058397524115 28.03966959131696 29.357630348280395 21.40464166287853 76-77 21.166144630080517 27.706754509960206 29.721369458680236 21.40573140127904 78-79 21.10379602587973 27.824679772587164 29.585152158616097 21.48637204291701 80-81 21.039968490992532 28.11758588695365 29.3883765388663 21.454069083187516 82-83 21.09051848277043 27.919608316273713 29.560834740914295 21.429038460041568 84-85 21.13751653095521 28.02731509265508 29.195671244349896 21.63949713203981 86-87 21.053745897913306 27.952490519275912 29.16023202087316 21.833531561937615 88-89 21.060673520602283 27.759373307013558 29.28726438299012 21.892688789394043 90-91 20.906346480500332 27.76217396110498 29.447697546135487 21.883782012259203 92-93 20.90651297870833 27.659940483279506 29.471752131462985 21.961794406549174 94-95 20.71483725535373 27.620509499405316 29.46099981941478 22.20365342582618 96-97 20.86623305456787 27.583380555330688 29.402076107329894 22.148310282771547 98-99 20.75075893204639 27.478555304740404 29.168911029812406 22.601774733400795 100-101 20.600132342065315 27.54894710209801 29.52107742010821 22.329843135728467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 272.5 2 250.5 3 311.5 4 252.0 5 212.5 6 404.5 7 911.5 8 1454.0 9 1700.0 10 1539.5 11 1336.0 12 1394.5 13 1536.0 14 1635.5 15 1710.0 16 1834.0 17 1940.5 18 1982.0 19 2049.5 20 2246.5 21 2508.5 22 2875.0 23 3240.5 24 3719.0 25 4411.0 26 5243.0 27 6466.5 28 7287.5 29 8056.5 30 9204.0 31 10405.5 32 11937.5 33 13118.5 34 14084.0 35 15230.5 36 16890.5 37 18429.0 38 19075.0 39 19496.0 40 19980.5 41 20824.5 42 21327.0 43 21982.5 44 23189.0 45 23737.5 46 23559.5 47 23823.0 48 25980.5 49 25303.5 50 22382.5 51 20755.5 52 19030.5 53 18119.0 54 17238.5 55 15693.0 56 14088.0 57 12658.0 58 11407.0 59 9949.5 60 8121.5 61 6662.0 62 5431.5 63 3941.0 64 2672.0 65 1939.0 66 1448.5 67 1106.5 68 820.5 69 559.0 70 399.5 71 288.5 72 220.0 73 216.0 74 198.0 75 143.0 76 100.0 77 67.0 78 48.0 79 39.5 80 28.5 81 17.5 82 12.5 83 6.0 84 2.5 85 1.5 86 1.5 87 1.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04156573613385724 2 0.0 3 0.0 4 0.0 5 0.006538430403078666 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004825984345129492 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.3351537153852383E-4 22-23 0.0021794768010262224 24-25 0.0 26-27 0.0017124460579491746 28-29 0.002490830629744254 30-31 7.783845717950794E-5 32-33 0.0 34-35 0.0 36-37 3.1135382871803175E-4 38-39 0.0 40-41 4.6703074307704765E-4 42-43 0.017591491322568795 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.5567691435901588E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5567691435901588E-4 84-85 7.783845717950794E-5 86-87 0.0 88-89 0.0 90-91 0.009807645604618 92-93 0.002412992172564746 94-95 0.0 96-97 0.0 98-99 9.340614861540953E-4 100-101 0.012609830063080286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 642356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.69817285334236 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7087907504463 56.66932185567979 2 10.309336189804256 13.958464467611728 3 2.712976757340724 5.509907083966576 4 1.127409180822527 3.0529416639907416 5 0.5950629554431056 2.014233740810406 6 0.3477583257279233 1.4125561947790732 7 0.2226688731464034 1.0551993104326924 8 0.16714684719560702 0.9052428922671524 9 0.12325883801312461 0.7509958309363172 >10 0.626517327350602 7.5775130336293905 >50 0.0368344101989935 1.697543549555009 >100 0.020154587212614372 2.534230325291501 >500 0.0011583096098752557 0.49715391614572335 >1k 6.949857659251534E-4 1.4611933869809792 >5k 2.3166192197505113E-4 0.9035027479228744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5761 0.8968547036222905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4748 0.7391539893766074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3325 0.5176257402437278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.19366208146261576 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 917 0.14275573046721757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5567691435901588E-4 2 0.0 0.0 0.0 0.0 1.5567691435901588E-4 3 0.0 0.0 0.0 0.0 1.5567691435901588E-4 4 0.0 0.0 0.0 0.0 1.5567691435901588E-4 5 0.0 0.0 0.0 0.0 1.5567691435901588E-4 6 0.0 0.0 0.0 0.0 1.5567691435901588E-4 7 0.0 0.0 0.0 0.0 1.5567691435901588E-4 8 0.0 0.0 0.0 0.0 1.5567691435901588E-4 9 0.0 0.0 0.0 0.0 1.5567691435901588E-4 10-11 1.5567691435901588E-4 0.0 0.0 0.0 1.5567691435901588E-4 12-13 3.1135382871803175E-4 0.0 0.0 0.0 1.5567691435901588E-4 14-15 4.670307430770476E-4 0.0 0.0 0.0 1.5567691435901588E-4 16-17 4.670307430770476E-4 0.0 0.0 0.0 1.5567691435901588E-4 18-19 4.670307430770476E-4 0.0 0.0 0.0 1.5567691435901588E-4 20-21 4.670307430770476E-4 0.0 0.0 7.783845717950794E-5 1.5567691435901588E-4 22-23 4.670307430770476E-4 0.0 0.0 3.891922858975397E-4 1.5567691435901588E-4 24-25 4.670307430770476E-4 0.0 0.0 9.340614861540952E-4 1.5567691435901588E-4 26-27 4.670307430770476E-4 0.0 0.0 0.0014010922292311428 1.5567691435901588E-4 28-29 6.227076574360635E-4 0.0 0.0 0.0021016383438467144 1.5567691435901588E-4 30-31 6.227076574360635E-4 0.0 0.0 0.004436792059231953 1.5567691435901588E-4 32-33 8.562230289745873E-4 0.0 0.0 0.00716113806051473 1.5567691435901588E-4 34-35 9.340614861540952E-4 0.0 0.0 0.010041160976156524 1.5567691435901588E-4 36-37 0.0010897384005131112 0.0 0.0 0.01206496086282373 1.5567691435901588E-4 38-39 0.001245415314872127 0.0 0.0 0.014789306864106507 1.5567691435901588E-4 40-41 0.001245415314872127 0.0 0.0 0.01883690663744092 1.5567691435901588E-4 42-43 0.0014789306864106508 0.0 0.0 0.025453175497699095 1.5567691435901588E-4 44-45 0.0015567691435901588 0.0 0.0 0.03814084401795889 1.5567691435901588E-4 46-47 0.0015567691435901588 0.0 0.0 0.050672835623859665 1.5567691435901588E-4 48-49 0.0015567691435901588 0.0 0.0 0.07916171095155958 1.5567691435901588E-4 50-51 0.0015567691435901588 0.0 0.0 0.11068628610926029 1.5567691435901588E-4 52-53 0.0015567691435901588 0.0 0.0 0.1383967768651651 1.5567691435901588E-4 54-55 0.0015567691435901588 0.0 0.0 0.18159712059979202 1.5567691435901588E-4 56-57 0.0015567691435901588 0.0 0.0 0.23110237936595907 1.5567691435901588E-4 58-59 0.0015567691435901588 0.0 0.0 0.29422936813854 1.5567691435901588E-4 60-61 0.0015567691435901588 0.0 0.0 0.36996618697420125 1.5567691435901588E-4 62-63 0.0015567691435901588 0.0 0.0 0.47512594262371644 1.5567691435901588E-4 64-65 0.0015567691435901588 0.0 0.0 0.5662747759809202 1.5567691435901588E-4 66-67 0.0018681229723081904 0.0 0.0 0.6884033152955682 1.5567691435901588E-4 68-69 0.0018681229723081904 0.0 0.0 0.8538100368020225 1.5567691435901588E-4 70-71 0.0019459614294876984 0.0 0.0 0.9736812608584648 1.5567691435901588E-4 72-73 0.0021016383438467144 0.0 0.0 1.1345733518485077 1.5567691435901588E-4 74-75 0.0021794768010262224 0.0 0.0 1.3459826015480512 1.5567691435901588E-4 76-77 0.0021794768010262224 0.0 0.0 1.5250888915180991 1.5567691435901588E-4 78-79 0.002335153715385238 0.0 0.0 1.6917410283394254 1.5567691435901588E-4 80-81 0.0031913767443598256 0.0 0.0 1.9247115306776927 1.5567691435901588E-4 82-83 0.003736245944616381 0.0 0.0 2.2135700452708464 1.5567691435901588E-4 84-85 0.003736245944616381 0.0 0.0 2.49775825243323 1.5567691435901588E-4 86-87 0.003736245944616381 0.0 0.0 2.8615752012902504 1.5567691435901588E-4 88-89 0.003736245944616381 0.0 0.0 3.284315862232158 1.5567691435901588E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3605 0.0 36.514225 1 GTATCAA 5395 0.0 32.41485 1 GTACACG 75 3.0688965E-4 31.680813 1 CCGACGA 30 0.0039515193 31.666021 74-75 ATACCGT 95 3.4889592E-5 29.999386 6 ATCAACG 6760 0.0 25.50812 3 TATCAAC 6855 0.0 25.154615 2 TCAACGC 6840 0.0 25.14033 4 CAACGCA 6945 0.0 24.828638 5 GGACCGA 115 1.281071E-4 24.782103 6 AACGCAG 7140 0.0 24.215189 6 GTACATG 3980 0.0 23.641212 1 GAGTACT 4735 0.0 23.375153 12-13 TACATGG 4045 0.0 22.899952 2 GTACTTT 4945 0.0 22.09257 14-15 ACGCAGA 7920 0.0 21.83206 7 GTATAGG 175 4.029009E-6 21.723986 1 CGCAGAG 7945 0.0 21.643784 8 ACATGGG 4010 0.0 21.559841 3 AGGACCG 155 3.901244E-5 21.452843 5 >>END_MODULE