FastQCFastQC Report
Thu 26 May 2016
SRR937656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533684
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.7191521574564724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.5786195576408512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.3796253963019315No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.14371800541144197No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7230.13547342622225886No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5770.10811641345815128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA459.621305E-442.221436
GTATCAA43750.032.8218421
GTGAGCG804.4884672E-429.689739
CGATACT650.005825771529.246694
GGTATCA29350.028.0267051
ACGATAC700.0083653527.157643
ATCAACG53200.026.5322993
TCAACGC53600.026.245634
TATCAAC54300.025.9972552
CAACGCA54200.025.952655
AACGCAG55800.025.196666
ACCGAGT1151.279839E-424.784478
GAGTACT28700.023.66902412-13
GTACATG36200.023.3800531
ACGCAGA60950.023.069827
CGCAGAG61050.022.954228
TACATGG36450.022.8197772
CATGGGG13050.022.579344
ACATGGG36400.022.1961483
GTACTTT30300.022.17984614-15
Produced by FastQC (version 0.11.5)