##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937656_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533684 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867076772022394 33.0 31.0 34.0 30.0 34.0 2 31.955250672682713 33.0 31.0 34.0 30.0 34.0 3 31.99153244241911 34.0 31.0 34.0 30.0 34.0 4 35.60777726145059 37.0 35.0 37.0 33.0 37.0 5 35.62128150740888 37.0 35.0 37.0 33.0 37.0 6 35.69743893390096 37.0 35.0 37.0 35.0 37.0 7 35.629936441789525 37.0 35.0 37.0 33.0 37.0 8 35.63451967831151 37.0 35.0 37.0 33.0 37.0 9 37.20124455670397 39.0 37.0 39.0 34.0 39.0 10-11 37.14811667578567 39.0 37.0 39.0 34.0 39.0 12-13 37.05030973384999 39.0 37.0 39.0 33.0 39.0 14-15 38.16365208625329 40.0 38.0 41.0 33.0 41.0 16-17 38.09692252344084 40.0 38.0 41.0 33.0 41.0 18-19 38.06860895211398 40.0 38.0 41.0 33.5 41.0 20-21 38.1032802182565 40.0 38.0 41.0 33.5 41.0 22-23 38.09954954617339 40.0 38.0 41.0 33.5 41.0 24-25 38.02661406375309 40.0 38.0 41.0 33.0 41.0 26-27 37.91992827216106 40.0 38.0 41.0 33.0 41.0 28-29 37.772569535530394 40.0 38.0 41.0 33.0 41.0 30-31 37.49796134041868 40.0 37.0 41.0 32.5 41.0 32-33 37.31734228494764 40.0 37.0 41.0 32.5 41.0 34-35 37.28263166967719 40.0 36.5 41.0 32.5 41.0 36-37 37.10710832627547 40.0 36.0 41.0 31.5 41.0 38-39 36.922092474198216 40.0 36.0 41.0 31.0 41.0 40-41 36.67521229791412 39.5 35.5 41.0 30.5 41.0 42-43 36.36779629893345 39.0 35.0 41.0 30.0 41.0 44-45 36.02862930123444 39.0 35.0 41.0 29.5 41.0 46-47 35.55035376739794 39.0 35.0 40.5 27.5 41.0 48-49 35.12471893480037 39.0 35.0 40.0 25.5 41.0 50-51 34.207433612399846 38.0 34.0 39.5 23.5 40.5 52-53 34.281468996634715 38.0 34.0 39.5 24.0 40.5 54-55 34.54015391130332 38.0 34.0 40.0 23.5 41.0 56-57 34.3156980535298 38.0 34.0 40.0 22.5 41.0 58-59 34.042595431004116 38.0 34.0 40.0 22.0 41.0 60-61 33.685688534788376 38.0 33.5 40.0 20.0 41.0 62-63 33.32987404531521 37.0 33.0 40.0 18.5 41.0 64-65 32.91521106122724 37.0 33.0 39.5 15.5 41.0 66-67 32.41359774698136 36.0 32.0 39.0 8.5 40.5 68-69 31.9555626550543 35.5 31.0 39.0 8.0 40.0 70-71 31.49319541151693 35.0 31.0 38.5 6.5 40.0 72-73 31.0139473920897 35.0 31.0 37.5 2.0 40.0 74-75 30.4757600002998 35.0 30.0 37.0 2.0 39.0 76-77 29.953372220265177 34.0 29.5 36.5 2.0 39.0 78-79 29.444083952301362 34.0 29.0 36.0 2.0 38.5 80-81 28.916168556673988 34.0 29.0 35.5 2.0 37.5 82-83 28.37759704244459 34.0 27.5 35.0 2.0 37.0 84-85 27.83942089326268 33.5 26.5 35.0 2.0 36.5 86-87 27.26218792394001 33.0 26.0 35.0 2.0 36.0 88-89 26.71701137751928 33.0 25.0 35.0 2.0 35.5 90-91 26.150445769406616 32.0 24.5 35.0 2.0 35.0 92-93 25.512335014727817 32.0 21.5 34.0 2.0 35.0 94-95 24.75591454868424 31.5 18.0 34.0 2.0 35.0 96-97 23.8880339301909 31.0 4.5 34.0 2.0 35.0 98-99 22.7253814991643 31.0 2.0 34.0 2.0 35.0 100-101 19.54186653525307 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24808618285196715 1101 2 0.15536006052591844 1101 3 0.12214217897835766 1101 4 0.19167164977861262 1101 5 0.15357143295275932 1101 6 0.34884377499700037 1101 7 0.22110715465489506 1101 8 0.2270574801837597 1101 9 0.45485984109811284 1101 10-11 0.39706370609025043 1101 12-13 0.3609604162572779 1101 14-15 0.6328553235655718 1101 16-17 0.3158278798235443 1101 18-19 0.5690518921900178 1101 20-21 0.3391453595417744 1101 22-23 0.4448168290457488 1101 24-25 0.3279740902446946 1101 26-27 0.5118376122100372 1101 28-29 0.48033205925495537 1101 30-31 0.4182056259204856 1101 32-33 0.5087196858238769 1101 34-35 0.6083273630960164 1101 36-37 0.5487901996222462 1101 38-39 0.6497925409430039 1101 40-41 0.7575640543224083 1101 42-43 0.5123656931579319 1101 44-45 0.7548369601409775 1101 46-47 0.7273316732323352 1101 48-49 0.8380659235062566 1101 50-51 0.8636265908055236 1101 52-53 1.0589708062583085 1101 54-55 1.0969917816194652 1101 56-57 1.299051587523195 1101 58-59 1.2522636731980796 1101 60-61 1.2807510821944845 1101 62-63 1.2203479492329663 1101 64-65 1.3793117157844321 1101 66-67 1.2740196447938033 1101 68-69 1.305975837779119 1101 70-71 1.4262622620278194 1101 72-73 1.5546775011992224 1101 74-75 1.5665719037972998 1101 76-77 1.560723750855125 1101 78-79 1.664831023095246 1101 80-81 1.8187900109465325 1101 82-83 1.8916069676859557 1101 84-85 2.1403564776139383 1101 86-87 2.4889092082497726 1101 88-89 2.27515642637886 1101 90-91 2.3200251831715875 1101 92-93 2.4153630467335674 1101 94-95 2.65345190888511 1101 96-97 2.844387619557036 1101 98-99 2.9129787412234265 1101 100-101 3.2948727891583225 1102 1 0.12432785892107745 1102 2 -0.018662638086944128 1102 3 -0.02995622592419167 1102 4 0.05289153429443871 1102 5 -0.010097512424550814 1102 6 0.1308618719417538 1102 7 0.0810881285271563 1102 8 0.07002555611128258 1102 9 0.19787437617477366 1102 10-11 0.15567524123327559 1102 12-13 0.11686183436749786 1102 14-15 0.21343982390848026 1102 16-17 0.2746672860313737 1102 18-19 0.1399242180235234 1102 20-21 0.2083628547299341 1102 22-23 0.14269961924950536 1102 24-25 0.10772453824363737 1102 26-27 0.15313979579004666 1102 28-29 0.06302934471323596 1102 30-31 0.34806337243052354 1102 32-33 0.31504034244651535 1102 34-35 0.10182308221727254 1102 36-37 0.03664445063439814 1102 38-39 0.2537061711724178 1102 40-41 0.4854904276327332 1102 42-43 0.3855432777246435 1102 44-45 0.6162117084872492 1102 46-47 0.5081993531509212 1102 48-49 0.6205486119423469 1102 50-51 0.37667939937171013 1102 52-53 0.43106434401190086 1102 54-55 0.5963391411911587 1102 56-57 0.7237877659263319 1102 58-59 0.8322729650279115 1102 60-61 0.8427740462881985 1102 62-63 0.9479535331802325 1102 64-65 0.8791457902518189 1102 66-67 1.027985265023453 1102 68-69 1.0485368430666107 1102 70-71 1.0414145816667641 1102 72-73 1.0205963967988723 1102 74-75 1.2402295662383978 1102 76-77 1.0066762740293917 1102 78-79 1.120628461205019 1102 80-81 1.3229934577729345 1102 82-83 1.4069163634957853 1102 84-85 1.3352747316348648 1102 86-87 1.3437686140151293 1102 88-89 1.7174696495376374 1102 90-91 1.568238718270365 1102 92-93 1.5840761283025593 1102 94-95 1.6134638555234595 1102 96-97 1.9546064200260531 1102 98-99 2.246776666048099 1102 100-101 2.622232803317303 1103 1 0.039648273475012985 1103 2 -0.0041528760043654245 1103 3 0.1924837425556305 1103 4 0.042407894275797275 1103 5 0.03621131416020518 1103 6 0.01571216047114632 1103 7 0.0916316643645132 1103 8 0.05395475925356408 1103 9 0.05822353136296243 1103 10-11 0.2926532351351412 1103 12-13 0.2227136558554932 1103 14-15 0.3271192802041867 1103 16-17 0.3774943686745047 1103 18-19 0.23717256667708142 1103 20-21 0.15575935194758728 1103 22-23 0.1049427925299824 1103 24-25 0.12247014011237667 1103 26-27 0.2754339553861982 1103 28-29 0.2194248143432489 1103 30-31 0.2568850906543858 1103 32-33 0.30069716560972637 1103 34-35 0.3046621440241424 1103 36-37 0.28292014734442716 1103 38-39 0.41400890710111327 1103 40-41 0.514107526765919 1103 42-43 0.4407709660642354 1103 44-45 0.45333438822617467 1103 46-47 0.5410746706455498 1103 48-49 0.518131441308519 1103 50-51 0.6653547846234886 1103 52-53 0.8046214630978952 1103 54-55 0.7040327591044999 1103 56-57 0.5418907156082327 1103 58-59 0.6502327262682073 1103 60-61 0.7614149352918815 1103 62-63 0.8363517130960361 1103 64-65 0.8713406163761235 1103 66-67 1.0538546434733078 1103 68-69 1.2095583288198348 1103 70-71 1.148325810448199 1103 72-73 0.9096253545554731 1103 74-75 1.0725727468186719 1103 76-77 1.1529690036128741 1103 78-79 1.3530766466424744 1103 80-81 1.4371689263173337 1103 82-83 1.3322019448876468 1103 84-85 1.5253793808606169 1103 86-87 1.6857100995437406 1103 88-89 1.6756230614467995 1103 90-91 1.614773090047457 1103 92-93 1.664034238664346 1103 94-95 1.6762449681257294 1103 96-97 1.9897624579925157 1103 98-99 2.3551207151177493 1103 100-101 3.4553686676233255 1104 1 0.2613334835947789 1104 2 0.20570569431058416 1104 3 0.22006101256788213 1104 4 0.1925055890832965 1104 5 0.085797756348164 1104 6 0.2893082405302678 1104 7 0.3991688440723351 1104 8 0.34061865874434005 1104 9 0.3921413462388941 1104 10-11 0.43659666326112045 1104 12-13 0.5310131587762186 1104 14-15 0.6899304609446446 1104 16-17 0.654064825359761 1104 18-19 0.612223438843678 1104 20-21 0.5412154803985416 1104 22-23 0.4958040254080771 1104 24-25 0.6330798974445528 1104 26-27 0.5769170941735737 1104 28-29 0.5213230295147966 1104 30-31 0.4500039341098443 1104 32-33 0.36313376522299734 1104 34-35 0.5788909624737855 1104 36-37 0.45822310248581033 1104 38-39 0.604537066490451 1104 40-41 0.6352308651949059 1104 42-43 0.5675778138121927 1104 44-45 0.4992529725119965 1104 46-47 0.6223142304621092 1104 48-49 0.6836730147517329 1104 50-51 0.6925594982714003 1104 52-53 0.60265136884518 1104 54-55 0.7798601278239374 1104 56-57 0.7846435831786138 1104 58-59 0.9521228423883557 1104 60-61 1.0819743772672439 1104 62-63 0.7637173953904934 1104 64-65 0.9933690237989126 1104 66-67 1.2132773835848738 1104 68-69 1.0698071721740483 1104 70-71 1.2327027403002973 1104 72-73 1.3490221777025653 1104 74-75 1.0169221398960104 1104 76-77 1.077531401741993 1104 78-79 1.132112252097187 1104 80-81 1.4841575977691903 1104 82-83 1.4744587455598683 1104 84-85 1.5139364336158145 1104 86-87 1.595610177497111 1104 88-89 1.542462507876639 1104 90-91 1.9510760755602803 1104 92-93 1.9601628920958127 1104 94-95 1.8814091257737644 1104 96-97 1.9545670177584533 1104 98-99 2.2833094635105944 1104 100-101 3.0154221213625547 1105 1 0.29052501311102574 1105 2 0.3111121498627476 1105 3 0.2102083856632717 1105 4 0.3466062246471111 1105 5 0.2830168177119887 1105 6 0.36375801988693723 1105 7 0.3300811140620681 1105 8 0.23340820108288085 1105 9 0.4756333392449221 1105 10-11 0.35183909339072983 1105 12-13 0.34510519063767475 1105 14-15 0.32983915068403036 1105 16-17 0.4127129339329372 1105 18-19 0.5852619334733049 1105 20-21 0.4978750235696978 1105 22-23 0.2812096483438893 1105 24-25 0.25371213079916544 1105 26-27 0.33017678721567023 1105 28-29 0.27155061131935554 1105 30-31 0.3880822555552683 1105 32-33 0.3332525518234277 1105 34-35 0.49327718544844146 1105 36-37 -0.026670461389741718 1105 38-39 0.22108531660840214 1105 40-41 0.05973754000270759 1105 42-43 0.09762693817732782 1105 44-45 0.26987826739695464 1105 46-47 0.014489024389554572 1105 48-49 -0.03949869849519416 1105 50-51 0.2805781488778223 1105 52-53 -0.04878766976046478 1105 54-55 0.018071667886658815 1105 56-57 0.01568213331599111 1105 58-59 0.21212395254349303 1105 60-61 0.36778520683067484 1105 62-63 0.3792789951240607 1105 64-65 0.4048188864172104 1105 66-67 0.7604131833128847 1105 68-69 0.5787883272904786 1105 70-71 0.5153066091909722 1105 72-73 0.5615017092170902 1105 74-75 0.7077369937718387 1105 76-77 0.6350096843319832 1105 78-79 0.5999968514699461 1105 80-81 0.8293062197891174 1105 82-83 1.015580279794282 1105 84-85 0.9756333057536999 1105 86-87 0.9089833839027044 1105 88-89 1.2115904810108837 1105 90-91 1.2166251860484678 1105 92-93 1.4526318771364721 1105 94-95 1.4442161530558266 1105 96-97 1.6801019737685543 1105 98-99 1.8213919480378031 1105 100-101 2.653464362765792 1106 1 -0.37297308574828847 1106 2 -0.4417395611638675 1106 3 -0.16636991981057747 1106 4 0.050638077721224306 1106 5 -0.14604014476738314 1106 6 -0.1755381993540439 1106 7 -0.2598553950131901 1106 8 -0.27090523603599337 1106 9 -0.1073353098690788 1106 10-11 -0.5432304955324341 1106 12-13 -0.775747273671108 1106 14-15 -0.6691385378158614 1106 16-17 -0.4975792319326189 1106 18-19 -0.19559573206942105 1106 20-21 -0.40424313411736534 1106 22-23 -0.5584309284550244 1106 24-25 -0.5957883713889416 1106 26-27 -0.4739608840663436 1106 28-29 -0.11464997695814105 1106 30-31 0.10532344932813231 1106 32-33 0.026451490054292037 1106 34-35 -0.16736471856189183 1106 36-37 -0.24112271764916215 1106 38-39 2.8389999606304173E-5 1106 40-41 0.13851381520179729 1106 42-43 0.2016605847803845 1106 44-45 0.14642966993659456 1106 46-47 0.30724227067695864 1106 48-49 0.4294328963107503 1106 50-51 0.31568647802625094 1106 52-53 0.4405094462449881 1106 54-55 0.48945591352838136 1106 56-57 0.33046888272413355 1106 58-59 -0.13206322013747496 1106 60-61 0.3408643373278437 1106 62-63 0.2120010340077627 1106 64-65 0.3822204216779923 1106 66-67 0.5251111530381181 1106 68-69 0.5691839797763087 1106 70-71 0.5285383690015735 1106 72-73 0.16143265835260934 1106 74-75 0.3620880949986116 1106 76-77 0.9455592216015134 1106 78-79 1.1644562818451547 1106 80-81 0.7138074393887983 1106 82-83 0.7385787014888052 1106 84-85 0.9268134478011909 1106 86-87 0.8061648531616186 1106 88-89 1.052925942665233 1106 90-91 1.6060048043855062 1106 92-93 1.532246627118539 1106 94-95 1.764827387721155 1106 96-97 1.7556274777501883 1106 98-99 1.816917093313652 1106 100-101 2.44358112150028 1107 1 0.05321739044218887 1107 2 0.20349853407422458 1107 3 0.3296543389495312 1107 4 0.10701723315698075 1107 5 0.26808833681392485 1107 6 0.2034375418858474 1107 7 0.1581365187334427 1107 8 0.2517587352128814 1107 9 0.21323971259273833 1107 10-11 0.2699995134522837 1107 12-13 0.4068560506775043 1107 14-15 0.708152567305433 1107 16-17 0.5725128615085282 1107 18-19 0.5567402966817028 1107 20-21 0.5082905586050046 1107 22-23 0.4085951272502797 1107 24-25 0.6106694003990256 1107 26-27 0.37895915187256435 1107 28-29 0.4428298074084367 1107 30-31 0.31004043046021224 1107 32-33 0.3106832959842123 1107 34-35 0.1585225231272389 1107 36-37 0.3322151080471585 1107 38-39 0.4509830171334812 1107 40-41 0.16098686102389337 1107 42-43 0.34577558927274765 1107 44-45 0.03198672294647764 1107 46-47 0.3623298717551151 1107 48-49 0.5650685656728314 1107 50-51 0.42231271342607357 1107 52-53 0.5726635342953088 1107 54-55 0.5806508820818479 1107 56-57 0.4561764298939437 1107 58-59 0.4162521437439253 1107 60-61 0.5043414262766319 1107 62-63 0.2557229567391275 1107 64-65 0.35933183227996324 1107 66-67 0.36087036238222936 1107 68-69 1.0579979959488206 1107 70-71 0.630121926953052 1107 72-73 0.8841977077686067 1107 74-75 0.9318769539384135 1107 76-77 1.0221090868306248 1107 78-79 1.4289666142800144 1107 80-81 1.409348769128247 1107 82-83 1.6158473447951849 1107 84-85 1.6355735556178992 1107 86-87 1.7405875845183125 1107 88-89 1.5331820073552613 1107 90-91 1.9811508061824519 1107 92-93 2.0753957735695714 1107 94-95 2.3811571271641014 1107 96-97 1.9967550608589058 1107 98-99 2.296926078039615 1107 100-101 3.038875371275658 1108 1 -0.013556640124946284 1108 2 0.06091295873003233 1108 3 0.05413330825608398 1108 4 0.025389091184010226 1108 5 0.044209342245274286 1108 6 0.16795061501961328 1108 7 0.2926382239248042 1108 8 0.30958998442343955 1108 9 0.2680963502079976 1108 10-11 0.5006424337503788 1108 12-13 0.24685857688692892 1108 14-15 0.4013033820079741 1108 16-17 0.3463318586033921 1108 18-19 0.500434246410137 1108 20-21 0.60100560218212 1108 22-23 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-0.3412925735622281 2316 66-67 -0.17493250685983952 2316 68-69 -0.10451820835504577 2316 70-71 -0.6372814953959178 2316 72-73 -0.5054401800544 2316 74-75 -0.4523573386897084 2316 76-77 -0.6969860648416599 2316 78-79 -0.5179308016736641 2316 80-81 -0.6419886260839256 2316 82-83 -0.8167794808897177 2316 84-85 -0.7747583085252181 2316 86-87 -1.4999188539917547 2316 88-89 -1.5849408860852385 2316 90-91 -1.3110948942755911 2316 92-93 -1.1637948754668095 2316 94-95 -1.1174449815559768 2316 96-97 -1.4999284582837795 2316 98-99 -1.9254509853084087 2316 100-101 -2.1217524914846884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2059.0 3 364.0 4 341.0 5 408.0 6 440.0 7 485.0 8 633.0 9 776.0 10 1014.0 11 1379.0 12 2025.0 13 2741.0 14 3149.0 15 4204.0 16 5675.0 17 7845.0 18 6487.0 19 4115.0 20 3859.0 21 3884.0 22 4209.0 23 4611.0 24 5247.0 25 5999.0 26 6766.0 27 7949.0 28 9442.0 29 11326.0 30 13904.0 31 17010.0 32 21747.0 33 28453.0 34 38751.0 35 54444.0 36 77228.0 37 100491.0 38 68281.0 39 5942.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79978651913435 19.233761547886616 14.642607473333685 21.323844459645354 2 14.958786977135603 20.468821615610604 41.8289640766705 22.743427330583295 3 19.60408984432969 25.903036200344516 29.813672763612935 24.679201191712863 4 12.100105380016117 16.590684106026195 37.93417599777594 33.37503451618175 5 12.80472914843019 38.76939953940769 33.77445253006435 14.65141878209776 6 30.599787835262532 36.81095746378648 17.670506285145652 14.918748415805332 7 27.76544934327618 34.30415061466946 20.195853140183686 17.73454690187067 8 26.28098614952208 35.216863149317426 19.488112471062696 19.0140382300978 9 27.07454817863325 17.115515318556124 20.611052491260217 35.198884011550405 10-11 24.76539793167156 27.65243971130521 26.60841826197325 20.97374409504998 12-13 26.050641063554338 25.195764483661492 27.796444366836496 20.957150085947674 14-15 22.882358352938084 26.03438920483882 27.159417133394292 23.923835308828803 16-17 20.92953179841339 29.77464331275363 27.548111357575266 21.747713531257716 18-19 20.934648565794006 27.550140186959748 31.147223997829848 20.367987249416398 20-21 22.03496421447847 26.391927724979467 31.396362783057608 20.176745277484454 22-23 21.427552271366164 26.541106498977758 31.26331776973001 20.76802345992607 24-25 21.071820476500243 26.70858877565101 31.599094722751804 20.620496025096944 26-27 20.867863244653886 27.04100407412275 31.44800330435762 20.64312937686574 28-29 20.7507953778432 27.209197750317543 31.47865646154727 20.561350410291986 30-31 20.952202137596636 26.909497143769094 31.394976600967876 20.743324117666386 32-33 20.530391926777753 27.130814362825628 31.271344754752405 21.067448955644217 34-35 21.178268608292715 27.179353826049734 31.139835377347865 20.50254218830969 36-37 21.185530623892344 26.87372337869094 31.13613664353729 20.804609353879428 38-39 20.8786495022926 27.183130980819143 31.155685503362633 20.782534013525627 40-41 21.075068586751563 27.279741026938193 31.084557633050792 20.560632753259455 42-43 20.92021735437041 27.316758607677986 30.80770108048288 20.955322957468717 44-45 20.895165128322585 27.63377867557539 30.53693574624785 20.934120449854177 46-47 21.242154630059005 27.842497451369503 29.92486468320945 20.99048323536204 48-49 21.253302140970202 27.975212585592807 29.59807288273386 21.173412390703124 50-51 21.39382503966131 28.445933894693365 29.29313695119547 20.86710411444986 52-53 21.51636708764505 28.469973723663816 29.095573953476144 20.918085235214985 54-55 21.083525852301264 28.47681746197887 29.215132788804265 21.2245238969156 56-57 21.268388460390547 28.701108798116902 28.985300494238263 21.045202247254284 58-59 21.453955449869607 28.258468456717196 29.09815874350502 21.189417349908176 60-61 21.18973273154053 28.43161270522383 29.389863564223923 20.988790999011716 62-63 21.247684277109677 28.30878557579147 29.48424369945061 20.95928644764824 64-65 21.064314038339706 28.29010535207302 29.549919050188883 21.09566155939839 66-67 21.17807164913137 28.337916600185835 29.194158446507174 21.289853304175622 68-69 21.186979936067214 28.44943284523423 29.273557617405636 21.09002960129292 70-71 21.13830981368232 28.387918445187765 29.078022171916825 21.395749569213095 72-73 21.17363819392625 28.422430906556663 28.978839033043773 21.425091866473313 74-75 20.988951059322233 28.881279503187212 28.73184518529574 21.397924252194816 76-77 21.113566261848398 28.57639012741544 28.9136552480255 21.39638836271066 78-79 21.0451175316063 28.627813920570546 28.851960768669755 21.475107779153404 80-81 21.074817672401654 28.794995259951754 28.772280573311182 21.357906494335406 82-83 21.141477213534944 28.847494882583323 28.704366768373664 21.30666113550807 84-85 21.15481534385526 28.66963673170214 28.58714187707234 21.588406047370253 86-87 21.195142095581335 28.690824792581747 28.59931673987311 21.51471637196381 88-89 21.27137551353325 28.401755938776795 28.565729909434186 21.761138638255776 90-91 21.065737098534782 28.486232461487777 28.699571351205744 21.74845908877169 92-93 21.253595746577883 28.444244224515362 28.71106856437618 21.59109146453057 94-95 20.917302378664104 28.64627132388275 28.48249414344065 21.95393215401249 96-97 21.0600763097255 28.387499472026807 28.593620206590042 21.958804011657648 98-99 21.02310004890141 28.44560164218624 28.239389755483575 22.291908553428776 100-101 21.442775937842836 27.869274004112405 28.832995325449506 21.854954732595253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 799.0 1 490.0 2 211.5 3 266.5 4 238.5 5 216.0 6 322.5 7 696.0 8 1183.5 9 1345.0 10 1170.0 11 1028.5 12 1061.5 13 1127.0 14 1198.5 15 1251.5 16 1288.0 17 1364.5 18 1447.5 19 1502.0 20 1622.0 21 1873.0 22 2179.0 23 2532.5 24 2961.5 25 3566.5 26 4344.5 27 5333.5 28 6141.5 29 6771.5 30 7735.0 31 8860.5 32 10090.0 33 11143.0 34 11914.0 35 12868.0 36 14218.0 37 15380.5 38 15980.5 39 16335.5 40 16701.0 41 17420.5 42 17949.5 43 18594.0 44 19188.0 45 19526.0 46 19544.5 47 19599.5 48 21342.5 49 21164.0 50 18874.5 51 17168.5 52 15736.5 53 15046.5 54 14260.5 55 13099.0 56 11766.5 57 10408.0 58 9400.0 59 8242.0 60 6775.5 61 5501.5 62 4423.0 63 3299.0 64 2320.5 65 1715.0 66 1270.0 67 957.0 68 715.0 69 499.5 70 371.5 71 261.5 72 201.5 73 186.0 74 152.0 75 101.5 76 72.0 77 50.0 78 34.0 79 25.0 80 17.0 81 12.5 82 7.5 83 3.5 84 3.5 85 4.5 86 3.0 87 0.5 88 1.0 89 3.0 90 3.0 91 1.0 92 0.0 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29080879321845887 2 0.24883639007352668 3 0.25127228847033073 4 0.2482742596742642 5 0.2484616364740183 6 0.20217956693474043 7 0.1948718717443281 8 0.1999310453376905 9 0.19899416133891965 10-11 0.20236694373449454 12-13 0.20470915373142157 14-15 0.20143005973572375 16-17 0.19477818334445102 18-19 0.18784524175354705 20-21 0.18672098095502207 22-23 0.19318548054654064 24-25 0.19290441534690939 26-27 0.19749514694088638 28-29 0.20152374813560084 30-31 0.20171112493535504 32-33 0.19843203093965717 34-35 0.20086792933646128 36-37 0.19262335014727816 38-39 0.18578409695625125 40-41 0.1807249233628889 42-43 0.18840737215280953 44-45 0.1915927777486303 46-47 0.19580875574309892 48-49 0.20143005973572375 50-51 0.19655826294211554 52-53 0.20152374813560084 54-55 0.1972140817412551 56-57 0.17707107576768275 58-59 0.16273675058648937 60-61 0.17660263376829732 62-63 0.17107501817554957 64-65 0.177352140967314 66-67 0.1778205829666994 68-69 0.17557206136964945 70-71 0.17575943816940362 72-73 0.18391032895870965 74-75 0.188969502552072 76-77 0.1905622053499824 78-79 0.18690835775477624 80-81 0.18531565495686586 82-83 0.17697738736780566 84-85 0.17304247457296829 86-87 0.17688369896792858 88-89 0.183160821759693 90-91 0.18053754656313475 92-93 0.17547837296977237 94-95 0.17763320616694522 96-97 0.18484721295748047 98-99 0.18353557535920134 100-101 0.16966969217739336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 533684.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.8482891864669 #Duplication Level Percentage of deduplicated Percentage of total 1 85.84816964366927 61.68044118687218 2 9.137752610149228 13.130637840967891 3 2.3373896562796217 5.038123438975041 4 0.914993833608402 2.6296296624372175 5 0.4685595930219808 1.6832602570268256 6 0.28351616171737604 1.2222090705664288 7 0.19155989372483515 0.9634275448609574 8 0.13472268260915568 0.7743675408063369 9 0.10875469937944524 0.7032455182261488 >10 0.5260755667750184 6.508269706244501 >50 0.03077241221111055 1.5133730962615375 >100 0.01616854860275811 2.0884054461665946 >500 7.823491259391816E-4 0.38728997462982717 >1k 7.823491259391816E-4 1.6773197159584967 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3838 0.7191521574564724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3088 0.5786195576408512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2026 0.3796253963019315 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 767 0.14371800541144197 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 723 0.13547342622225886 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 577 0.10811641345815128 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8737679975416163E-4 0.0 10-11 9.368839987708082E-5 0.0 0.0 3.7475359950832327E-4 0.0 12-13 1.8737679975416163E-4 0.0 0.0 3.7475359950832327E-4 9.368839987708082E-5 14-15 1.8737679975416163E-4 0.0 0.0 4.6844199938540407E-4 2.8106519963124247E-4 16-17 1.8737679975416163E-4 0.0 0.0 8.431955988937273E-4 3.7475359950832327E-4 18-19 2.8106519963124247E-4 0.0 0.0 0.001030572398647889 3.7475359950832327E-4 20-21 3.7475359950832327E-4 0.0 0.0 0.0013116375982791316 3.7475359950832327E-4 22-23 3.7475359950832327E-4 0.0 0.0 0.0013116375982791316 4.6844199938540407E-4 24-25 3.7475359950832327E-4 0.0 0.0 0.0018737679975416163 5.621303992624849E-4 26-27 3.7475359950832327E-4 0.0 0.0 0.002623275196558263 5.621303992624849E-4 28-29 3.7475359950832327E-4 0.0 0.0 0.0032790939956978287 5.621303992624849E-4 30-31 3.7475359950832327E-4 0.0 0.0 0.004403354794222799 5.621303992624849E-4 32-33 4.6844199938540407E-4 0.0 0.0 0.006651876391272738 5.621303992624849E-4 34-35 5.621303992624849E-4 0.0 0.0 0.008619332788691435 5.621303992624849E-4 36-37 5.621303992624849E-4 0.0 0.0 0.01011834718672473 5.621303992624849E-4 38-39 5.621303992624849E-4 0.0 0.0 0.013116375982791315 5.621303992624849E-4 40-41 5.621303992624849E-4 0.0 0.0 0.015458585979718335 5.621303992624849E-4 42-43 5.621303992624849E-4 0.0 0.0 0.02154833197172859 5.621303992624849E-4 44-45 5.621303992624849E-4 0.0 0.0 0.03550790355341363 5.621303992624849E-4 46-47 5.621303992624849E-4 0.0 0.0 0.04862427953620495 5.621303992624849E-4 48-49 5.621303992624849E-4 0.0 0.0 0.07776137189797708 5.621303992624849E-4 50-51 5.621303992624849E-4 0.0 0.0 0.10830379025790543 5.621303992624849E-4 52-53 5.621303992624849E-4 0.0 0.0 0.13753457101955463 5.621303992624849E-4 54-55 5.621303992624849E-4 0.0 0.0 0.17800795976645356 5.621303992624849E-4 56-57 5.621303992624849E-4 0.0 0.0 0.22991133329835634 5.621303992624849E-4 58-59 5.621303992624849E-4 0.0 0.0 0.2968985392104691 5.621303992624849E-4 60-61 5.621303992624849E-4 0.0 0.0 0.3696007375150838 5.621303992624849E-4 62-63 5.621303992624849E-4 0.0 0.0 0.4946747513509867 5.621303992624849E-4 64-65 5.621303992624849E-4 0.0 0.0 0.592391752422782 5.621303992624849E-4 66-67 5.621303992624849E-4 0.0 0.0 0.7333927942377887 5.621303992624849E-4 68-69 7.495071990166465E-4 0.0 0.0 0.9213317243912128 5.621303992624849E-4 70-71 7.495071990166465E-4 0.0 0.0 1.0457499194279762 5.621303992624849E-4 72-73 7.495071990166465E-4 0.0 0.0 1.2203850967988548 5.621303992624849E-4 74-75 7.495071990166465E-4 0.0 0.0 1.4695025520720126 5.621303992624849E-4 76-77 7.495071990166465E-4 0.0 0.0 1.664561800616095 5.621303992624849E-4 78-79 7.495071990166465E-4 0.0 0.0 1.842007629983286 5.621303992624849E-4 80-81 7.495071990166465E-4 0.0 0.0 2.10086867884366 5.621303992624849E-4 82-83 7.495071990166465E-4 0.0 0.0 2.4373974112021344 5.621303992624849E-4 84-85 8.431955988937273E-4 0.0 0.0 2.7689606583671234 5.621303992624849E-4 86-87 9.368839987708081E-4 0.0 0.0 3.196741892205875 5.621303992624849E-4 88-89 9.368839987708081E-4 0.0 0.0 3.674552731578987 5.621303992624849E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 45 9.621305E-4 42.22143 6 GTATCAA 4375 0.0 32.821842 1 GTGAGCG 80 4.4884672E-4 29.68973 9 CGATACT 65 0.0058257715 29.24669 4 GGTATCA 2935 0.0 28.026705 1 ACGATAC 70 0.00836535 27.15764 3 ATCAACG 5320 0.0 26.532299 3 TCAACGC 5360 0.0 26.24563 4 TATCAAC 5430 0.0 25.997255 2 CAACGCA 5420 0.0 25.95265 5 AACGCAG 5580 0.0 25.19666 6 ACCGAGT 115 1.279839E-4 24.78447 8 GAGTACT 2870 0.0 23.669024 12-13 GTACATG 3620 0.0 23.380053 1 ACGCAGA 6095 0.0 23.06982 7 CGCAGAG 6105 0.0 22.95422 8 TACATGG 3645 0.0 22.819777 2 CATGGGG 1305 0.0 22.57934 4 ACATGGG 3640 0.0 22.196148 3 GTACTTT 3030 0.0 22.179846 14-15 >>END_MODULE