FastQCFastQC Report
Thu 26 May 2016
SRR937656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533684
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48910.9164599275976046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.7691817629908335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29370.5503256608779727No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.20517759573080702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.14146948381439203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAC1251.3460522E-1037.9987645
GGTATCA30800.037.6356961
GTATCAA48150.034.1382061
GGCGACA1605.2750693E-1132.655196
GCGACAG1551.3533281E-930.6441657
GCGTAGA650.005836883629.2352961
AGGACCG1153.8604467E-628.9121025
AAGGCGA1703.6325218E-927.9402684
TATCAAC60250.027.2771222
TGAGCCG700.00838894427.1419735
ATCAACG61100.026.8976523
TCAACGC61200.026.776094
GTACATG31850.026.4012641
CAACGCA62050.026.2561955
AACGCAG63250.025.833156
TACATGG32150.025.7067832
CGACAGC2059.549694E-1025.4869758
GTAAGGT2251.0186341E-1025.3325084
GAGTACT43500.024.5135712-13
ACATGGG32050.024.4531843
Produced by FastQC (version 0.11.5)