##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937656_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533684 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.259927222850976 33.0 31.0 34.0 31.0 34.0 2 32.46398243155126 34.0 31.0 34.0 31.0 34.0 3 32.56361629728453 34.0 31.0 34.0 31.0 34.0 4 36.177436460527204 37.0 35.0 37.0 35.0 37.0 5 36.04225346834456 37.0 35.0 37.0 35.0 37.0 6 36.14669167522354 37.0 35.0 37.0 35.0 37.0 7 36.11237174058057 37.0 35.0 37.0 35.0 37.0 8 36.10950112800833 37.0 35.0 37.0 35.0 37.0 9 37.77459133119974 39.0 38.0 39.0 35.0 39.0 10-11 37.73430250860059 39.0 37.0 39.0 35.0 39.0 12-13 37.65710326710188 39.0 37.0 39.0 35.0 39.0 14-15 38.85543505145367 40.0 38.0 41.0 35.0 41.0 16-17 38.840861820852794 40.0 38.0 41.0 35.5 41.0 18-19 38.88255315879809 40.0 38.0 41.0 35.0 41.0 20-21 38.83237552559193 40.0 38.0 41.0 35.0 41.0 22-23 38.696993914001546 40.0 38.0 41.0 34.5 41.0 24-25 38.62554995090728 40.0 38.0 41.0 34.0 41.0 26-27 38.53025104743631 40.0 38.0 41.0 34.0 41.0 28-29 38.40879621648766 40.0 38.0 41.0 34.0 41.0 30-31 38.39755641915441 40.0 38.0 41.0 34.0 41.0 32-33 38.232029628019575 40.0 37.0 41.0 34.0 41.0 34-35 38.06778449419507 40.0 37.0 41.0 34.0 41.0 36-37 37.868632936344355 40.0 37.0 41.0 33.0 41.0 38-39 37.69782493010845 40.0 37.0 41.0 33.0 41.0 40-41 37.481550880296204 40.0 37.0 41.0 33.0 41.0 42-43 37.219201812308405 40.0 36.0 41.0 33.0 41.0 44-45 36.79709434796621 40.0 36.0 41.0 31.5 41.0 46-47 36.265943891891084 40.0 36.0 41.0 30.5 41.0 48-49 35.78670524130384 39.0 35.5 41.0 28.5 41.0 50-51 35.47831581984845 39.0 35.0 41.0 27.0 41.0 52-53 35.243713508368245 39.0 35.0 40.5 26.5 41.0 54-55 35.0242353152802 39.0 35.0 40.0 26.0 41.0 56-57 34.75009275151588 38.5 35.0 40.0 25.0 41.0 58-59 34.501589892145915 38.0 34.5 40.0 24.0 41.0 60-61 34.16330543917374 38.0 34.0 40.0 23.0 41.0 62-63 33.80783759678012 37.5 34.0 40.0 22.0 41.0 64-65 33.41543122896695 37.0 33.0 40.0 21.5 41.0 66-67 32.99605478148118 36.0 33.0 39.0 19.5 40.5 68-69 32.55195864968783 36.0 32.5 39.0 18.0 40.0 70-71 32.16149912682411 35.0 32.0 38.5 15.5 40.0 72-73 31.727033225654132 35.0 31.5 38.0 11.5 40.0 74-75 31.256413907855585 35.0 31.0 37.0 7.5 39.0 76-77 30.034172843855167 34.0 29.5 36.0 7.0 38.5 78-79 30.5669197502642 34.0 31.0 36.0 4.5 39.0 80-81 30.36068628626678 34.0 31.0 36.0 2.0 37.5 82-83 30.014660360812766 34.0 30.5 35.5 2.0 37.0 84-85 29.619157591383665 34.0 30.0 35.0 2.0 37.0 86-87 29.200040660765545 34.0 29.5 35.0 2.0 36.0 88-89 28.76687515458586 34.0 29.0 35.0 2.0 36.0 90-91 28.266158438326798 34.0 29.0 35.0 2.0 35.0 92-93 27.843139385853803 33.5 28.0 35.0 2.0 35.0 94-95 27.398656320968964 33.0 27.0 35.0 2.0 35.0 96-97 26.881940436662894 33.0 26.0 35.0 2.0 35.0 98-99 26.107975880858334 33.0 24.5 34.5 2.0 35.0 100-101 23.7720308272311 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15929313001379342 1101 2 0.21574688795899277 1101 3 0.18661860135854624 1101 4 0.08247288924355445 1101 5 -0.058745755097938 1101 6 0.06344215193866631 1101 7 -0.041298104511277245 1101 8 0.006009681843373471 1101 9 0.2904710366910308 1101 10-11 0.11936619866572329 1101 12-13 0.200201706915081 1101 14-15 0.2859999618917968 1101 16-17 0.15967882054132332 1101 18-19 0.1807831367784658 1101 20-21 0.21304158286132235 1101 22-23 0.02077417599119258 1101 24-25 -0.01764516195733279 1101 26-27 0.14957096938226755 1101 28-29 0.25483115659299926 1101 30-31 0.20326812273555106 1101 32-33 0.22361329133850205 1101 34-35 0.21177596666432663 1101 36-37 0.2158148038083496 1101 38-39 0.3710445678608636 1101 40-41 0.516982812443409 1101 42-43 0.4435182463247074 1101 44-45 0.5097956676491506 1101 46-47 0.5755752354607111 1101 48-49 0.7586106056813264 1101 50-51 0.8515323936139154 1101 52-53 0.751288589592292 1101 54-55 0.9070138673205577 1101 56-57 0.9316252121264696 1101 58-59 0.8434079664473089 1101 60-61 0.791566169125467 1101 62-63 0.6417903140121979 1101 64-65 0.709662926515314 1101 66-67 0.889621075196871 1101 68-69 0.8953272730456874 1101 70-71 0.7844728317230896 1101 72-73 0.6232731755109171 1101 74-75 0.6786281228885898 1101 76-77 0.6756418956060912 1101 78-79 0.7221929085895553 1101 80-81 0.6239940228643874 1101 82-83 0.8052918557892532 1101 84-85 0.851828846012399 1101 86-87 0.9299009443547241 1101 88-89 1.0062370049462181 1101 90-91 0.8623176536149977 1101 92-93 1.0787996330943876 1101 94-95 1.1272990345668141 1101 96-97 1.1201014638401574 1101 98-99 1.595145033389258 1101 100-101 2.1029407205744803 1102 1 0.2654633474868575 1102 2 0.2501709051267511 1102 3 0.1725481161922886 1102 4 0.03855793160987275 1102 5 -0.0058929421070743615 1102 6 -0.009476588797156182 1102 7 0.1302910196231437 1102 8 0.24755301053079393 1102 9 0.2884578068952308 1102 10-11 0.23742774597662475 1102 12-13 0.22036718997961202 1102 14-15 0.294473225757649 1102 16-17 0.1662715950374647 1102 18-19 0.2557704158209617 1102 20-21 0.29372563852380296 1102 22-23 0.38129717327001345 1102 24-25 0.33486916294697977 1102 26-27 0.169404601381558 1102 28-29 0.08555392821309482 1102 30-31 0.3078122699889292 1102 32-33 0.3942289856815435 1102 34-35 0.37232551202837527 1102 36-37 0.42754684513487007 1102 38-39 0.3386116570845559 1102 40-41 0.43173027378276174 1102 42-43 0.5796988617978158 1102 44-45 0.49488449311761684 1102 46-47 0.36352240477112474 1102 48-49 0.42952496598308443 1102 50-51 0.3017657955297963 1102 52-53 0.4121589243193924 1102 54-55 0.41906006098884063 1102 56-57 0.4806285527605212 1102 58-59 0.5635137163201023 1102 60-61 0.6467353025517255 1102 62-63 0.6128639628252728 1102 64-65 0.5527084343580597 1102 66-67 0.580247388774012 1102 68-69 0.596948918584502 1102 70-71 0.8830436598021087 1102 72-73 0.6736371055124692 1102 74-75 0.5660089330476588 1102 76-77 0.5476580014444608 1102 78-79 0.691773227609044 1102 80-81 0.4815524839603782 1102 82-83 0.755746491748635 1102 84-85 0.6899076495786929 1102 86-87 0.6891098114151895 1102 88-89 0.8901444406224215 1102 90-91 0.854994806775327 1102 92-93 1.2124581804716534 1102 94-95 1.1349979792804241 1102 96-97 1.3508596329072127 1102 98-99 1.6612829506919695 1102 100-101 2.2260021572683577 1103 1 0.2289718199375912 1103 2 0.19826556298124842 1103 3 0.1422707798342131 1103 4 0.15771691944540578 1103 5 0.10138982792479823 1103 6 0.0922920224247008 1103 7 0.18249958435087166 1103 8 0.1431808029393764 1103 9 0.23511496510067786 1103 10-11 0.19844492671786895 1103 12-13 0.17752670564705397 1103 14-15 0.2718653618109954 1103 16-17 0.10413997771933481 1103 18-19 0.19861951017746549 1103 20-21 0.08108425163612054 1103 22-23 0.10043444887125474 1103 24-25 0.18307350501949315 1103 26-27 0.16323158856572206 1103 28-29 0.29320524470446685 1103 30-31 0.342274826679386 1103 32-33 0.3349620730676719 1103 34-35 0.31274788985493984 1103 36-37 0.3448644108939263 1103 38-39 0.4524389811827021 1103 40-41 0.409322686479932 1103 42-43 0.5349913124913925 1103 44-45 0.6431163498318355 1103 46-47 0.8926710792953756 1103 48-49 0.8348568168949058 1103 50-51 1.024765415565696 1103 52-53 1.0737241518018408 1103 54-55 1.1787702026611697 1103 56-57 1.3037159576057178 1103 58-59 1.2677406765511492 1103 60-61 1.2083213063962859 1103 62-63 1.396583904950944 1103 64-65 1.4148844120169173 1103 66-67 1.2920081199522144 1103 68-69 0.8162415489655288 1103 70-71 1.1277180792338122 1103 72-73 1.2410854126133017 1103 74-75 1.245616840044427 1103 76-77 1.1048482137189808 1103 78-79 0.9415743547734827 1103 80-81 1.1464643903132696 1103 82-83 1.2680213910955835 1103 84-85 1.2963818260579814 1103 86-87 1.3745641118413836 1103 88-89 1.4154504240275365 1103 90-91 1.5309752732514497 1103 92-93 1.530921440937984 1103 94-95 1.571147164790876 1103 96-97 1.6881535772527627 1103 98-99 1.9284585446432416 1103 100-101 2.6543003111669243 1104 1 0.14156685724384488 1104 2 0.15530009531164524 1104 3 0.08886361014896238 1104 4 0.1187888288980119 1104 5 -0.06869227620875051 1104 6 -0.011446702177181578 1104 7 0.18284379403531403 1104 8 0.02695941536939017 1104 9 0.2410292973845074 1104 10-11 0.23071458463418537 1104 12-13 0.1946936574678162 1104 14-15 0.17134803490291262 1104 16-17 0.13732077891799577 1104 18-19 0.13157151317411575 1104 20-21 -0.005515867824883003 1104 22-23 0.10639838784490507 1104 24-25 0.10473036845935724 1104 26-27 0.14084682288140016 1104 28-29 0.1749064504507487 1104 30-31 0.3150721377943171 1104 32-33 0.19795447008316813 1104 34-35 0.1558652147382631 1104 36-37 0.26372305207558355 1104 38-39 0.11013662334636365 1104 40-41 0.31196905152442156 1104 42-43 0.5828293719586739 1104 44-45 0.39656401477407854 1104 46-47 0.29555940350040544 1104 48-49 0.16731174061197152 1104 50-51 -0.15634860895418257 1104 52-53 -0.02394635853963223 1104 54-55 0.0984802566210945 1104 56-57 0.17164877597436856 1104 58-59 0.1407303401368054 1104 60-61 0.26853066687494476 1104 62-63 0.4345775674432133 1104 64-65 0.3022863375663931 1104 66-67 0.09572558454416935 1104 68-69 0.062070031304536144 1104 70-71 0.2187352188513323 1104 72-73 0.30234461262950063 1104 74-75 0.29753074712805727 1104 76-77 -0.25693236273099984 1104 78-79 0.10870536705366973 1104 80-81 0.14742716504827413 1104 82-83 0.3621414797801954 1104 84-85 0.4803457042887409 1104 86-87 0.666620967182201 1104 88-89 0.5260389213497838 1104 90-91 0.567650815128605 1104 92-93 0.9400693194927037 1104 94-95 0.8145570679059766 1104 96-97 0.9684202385200642 1104 98-99 1.2228509639311298 1104 100-101 1.8606712274733503 1105 1 0.012922079136103548 1105 2 0.07135848947223167 1105 3 0.0324871565395739 1105 4 -0.15142015780788398 1105 5 -0.12039497666113874 1105 6 0.044768778936308706 1105 7 -0.010572985959690584 1105 8 -0.13165400085552648 1105 9 -0.10165061713354362 1105 10-11 0.034203247174772855 1105 12-13 -0.0012029315805008878 1105 14-15 0.1207985081065388 1105 16-17 -0.06127903790640943 1105 18-19 0.06881721826140108 1105 20-21 0.018763225178361154 1105 22-23 0.008869870734642404 1105 24-25 -0.14546390869087134 1105 26-27 -0.006157145923239682 1105 28-29 -0.09609352179536756 1105 30-31 -0.03608381124956139 1105 32-33 -0.06273493626137139 1105 34-35 -0.09206850899963115 1105 36-37 -0.3440327693004548 1105 38-39 -0.2828291561213234 1105 40-41 -0.5455522495774048 1105 42-43 -0.6159133773405401 1105 44-45 -0.34820854219075414 1105 46-47 0.034956034179536744 1105 48-49 -0.21900004646027327 1105 50-51 -0.3048610010609778 1105 52-53 -0.28888349600459406 1105 54-55 -0.3619316109373969 1105 56-57 -0.38834539571112714 1105 58-59 -0.18166287681526683 1105 60-61 0.06895530121759208 1105 62-63 -0.3934718899684526 1105 64-65 -0.2281652180385052 1105 66-67 -0.048688947386665404 1105 68-69 0.1189210762380668 1105 70-71 -0.09906611888561301 1105 72-73 0.047258714375221444 1105 74-75 0.025079247030920016 1105 76-77 -0.1968438278582525 1105 78-79 0.2885338480838975 1105 80-81 0.16442502799962 1105 82-83 0.260764887401411 1105 84-85 0.07774932894528774 1105 86-87 0.23218950538541705 1105 88-89 0.3599930164345757 1105 90-91 0.23589899031108885 1105 92-93 0.5317625836263673 1105 94-95 0.5605841001837355 1105 96-97 0.7216410761321228 1105 98-99 0.9049830168855308 1105 100-101 1.7913670172099678 1106 1 -0.40062085794690105 1106 2 -0.2729811331692744 1106 3 -0.2741614552690237 1106 4 -0.12882169306865876 1106 5 -0.17202170741657596 1106 6 -0.2555935139111014 1106 7 -0.5452239652264979 1106 8 -0.5945458869305682 1106 9 -0.49089123697714854 1106 10-11 -0.27917558200952897 1106 12-13 -0.49254467058617024 1106 14-15 -0.6150415808524983 1106 16-17 -0.44742133649310034 1106 18-19 -0.2351186810140149 1106 20-21 -0.5398448170054451 1106 22-23 -0.2207242536645282 1106 24-25 -0.517661519710316 1106 26-27 -0.46442621113595095 1106 28-29 -0.31419112564069707 1106 30-31 -0.42300629492844877 1106 32-33 -0.585472399494023 1106 34-35 -0.8949955482420435 1106 36-37 -0.4143929099131256 1106 38-39 -0.39175016910647287 1106 40-41 -0.603164883195312 1106 42-43 -0.3996317215546057 1106 44-45 0.06598454913574159 1106 46-47 -0.03374957597900874 1106 48-49 -0.25792410844463376 1106 50-51 -0.2114271105379899 1106 52-53 0.07145888867149353 1106 54-55 0.09330190653157899 1106 56-57 -0.21950258058808458 1106 58-59 -0.10405687925446472 1106 60-61 -0.2901854190636328 1106 62-63 -0.17986259231439305 1106 64-65 -0.16222612628156696 1106 66-67 0.01254661470530749 1106 68-69 -0.192040972689405 1106 70-71 -0.05775217957863177 1106 72-73 0.12066337037636998 1106 74-75 0.3788797348707931 1106 76-77 0.21920556592896645 1106 78-79 0.32040485748198577 1106 80-81 0.14190278824067093 1106 82-83 0.1471986344420877 1106 84-85 -0.03527886532976865 1106 86-87 0.04286907350364544 1106 88-89 0.3398920407548296 1106 90-91 0.5354272921978804 1106 92-93 0.049074621792666306 1106 94-95 0.2691311623833208 1106 96-97 0.5631110524575362 1106 98-99 0.9737065623335539 1106 100-101 1.643400879036495 1107 1 0.11907815373324127 1107 2 0.18088229899603903 1107 3 0.030712758567460696 1107 4 0.040495467757935444 1107 5 -0.18534156366096255 1107 6 0.03128878617874875 1107 7 -0.013732500716166385 1107 8 -0.006495572465155419 1107 9 0.0019434440650769602 1107 10-11 0.003472665968914157 1107 12-13 0.1457190311209331 1107 14-15 0.14520100674858583 1107 16-17 -0.03912169588215164 1107 18-19 0.008917707126151697 1107 20-21 0.048733350110460094 1107 22-23 0.11592219736871812 1107 24-25 -0.0048021306716776735 1107 26-27 -0.11639161994098401 1107 28-29 -0.040752221777268005 1107 30-31 -0.19170575584489313 1107 32-33 -0.048960719350269244 1107 34-35 0.13319402947583825 1107 36-37 -0.02389425762202535 1107 38-39 0.14585090906064124 1107 40-41 0.22999998652615972 1107 42-43 0.24973934762778072 1107 44-45 0.04636415380780079 1107 46-47 0.23533173579097877 1107 48-49 -0.061259687959456244 1107 50-51 -0.2992752686691631 1107 52-53 -0.04798181213713093 1107 54-55 -0.09706197487004431 1107 56-57 0.08094665571020698 1107 58-59 0.08089370475271807 1107 60-61 0.1884646258599929 1107 62-63 0.10142671586170593 1107 64-65 0.10578824927997488 1107 66-67 0.39389207741869825 1107 68-69 0.3581162600149952 1107 70-71 0.35895941071786197 1107 72-73 0.06950658690826472 1107 74-75 0.13995340962371472 1107 76-77 -0.04262057962359478 1107 78-79 0.12929973618818735 1107 80-81 -0.13375983529930835 1107 82-83 0.0679982750530499 1107 84-85 0.26341834940518183 1107 86-87 0.18443466200319492 1107 88-89 0.3314351674394622 1107 90-91 0.5263751807872801 1107 92-93 0.8151909739939249 1107 94-95 0.5606293654381282 1107 96-97 0.9247462725833273 1107 98-99 1.1938885030343656 1107 100-101 1.7239489473204124 1108 1 0.0674550641791214 1108 2 0.14695530644836197 1108 3 0.026380309407421976 1108 4 -0.06163286368074239 1108 5 -0.06852963995248729 1108 6 0.13701696604486102 1108 7 -0.008989542524929561 1108 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-0.22471893116986408 2316 80-81 -0.1054455150158411 2316 82-83 -0.20963114332046828 2316 84-85 -0.7052014760204486 2316 86-87 -0.3240131817441032 2316 88-89 -0.22721793082343567 2316 90-91 -0.3462244216671344 2316 92-93 -0.21664508166245966 2316 94-95 -0.3756121002951751 2316 96-97 -0.353686879261808 2316 98-99 -0.4162683546270287 2316 100-101 -1.122222515820198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 10.0 6 24.0 7 39.0 8 134.0 9 272.0 10 455.0 11 833.0 12 1394.0 13 2081.0 14 2785.0 15 4084.0 16 5866.0 17 8684.0 18 7576.0 19 4020.0 20 2995.0 21 2780.0 22 2846.0 23 2932.0 24 3395.0 25 3926.0 26 4481.0 27 5470.0 28 7035.0 29 8656.0 30 10574.0 31 13641.0 32 17674.0 33 23658.0 34 33207.0 35 49260.0 36 76775.0 37 119913.0 38 97719.0 39 8480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93225358977243 19.54110898661568 15.521501143478424 21.005136280133467 2 15.428043561358407 20.791329700721775 41.1029747940729 22.67765194384692 3 19.797295778025948 25.648885857548663 30.4637201040316 24.09009826039379 4 12.328104886233964 17.19672539691165 37.72164374731816 32.75352596953623 5 12.643708316389729 38.49893091319315 33.95423446451025 14.90312630590687 6 30.499134319185135 37.14857481206107 17.191259247045068 15.161031621708727 7 27.368067995293092 34.57364282983938 20.187976405513375 17.87031276935415 8 26.53367910598781 34.88450094063153 19.351713748210553 19.2301062051701 9 26.815681189617823 16.903448482622675 20.595333568178923 35.68553675958058 10-11 24.79266757107202 27.615967501367848 26.436055793315894 21.155309134244234 12-13 26.131244612674738 25.099970018363756 27.895195442791294 20.873589926170222 14-15 22.7464192293567 25.817806042527035 27.721273262829687 23.714501465286574 16-17 20.661852332091648 29.27509537479108 28.264103851717497 21.79894844139978 18-19 20.495086980310447 26.733703839725383 32.580328434054614 20.190880745909563 20-21 21.400792791982514 25.410566375014405 33.096957278986515 20.091683554016566 22-23 20.932522487663523 25.634203171741948 33.0088264254991 20.424447915095424 24-25 20.60841417884229 25.808790807268366 33.26706421889419 20.31573079499515 26-27 20.56577452028433 26.09769419806605 33.042457448393264 20.294073833256352 28-29 20.173983922648826 26.30792437273971 33.26531377068226 20.25277793392921 30-31 20.383897947004172 26.239990556200443 33.03765246630259 20.338459030492793 32-33 20.30686698495739 26.081632576580898 32.99902189310529 20.612478545356428 34-35 20.770624564348942 26.097091162560616 32.833099736922975 20.29918453616747 36-37 20.937764669729653 25.90090765321801 32.81848434654215 20.342843330510192 38-39 20.522069239475048 26.25514349315325 32.927818709198704 20.294968558173 40-41 20.627281312537008 26.273319042729405 32.88706425525218 20.212335389481417 42-43 20.4723354415554 26.403466854064185 32.46427734832513 20.659920356055284 44-45 20.58484046739269 26.659315250222974 32.02381933878475 20.732024943599583 46-47 20.967744957690318 26.993314395784772 31.26522436498003 20.773716281544885 48-49 20.961655211698307 27.375094625283875 30.737383920072553 20.92586624294526 50-51 21.20833676857466 27.638921159337738 30.486580073601605 20.666161998485997 52-53 21.216320159795085 27.83054001107398 30.026504473156844 20.926635355974092 54-55 20.84866700144655 27.733359066413833 30.35747745857099 21.06049647356863 56-57 21.310269747640927 27.853373906656376 29.920140007944777 20.916216337757923 58-59 21.16795706822764 27.466815568763536 30.165978369222234 21.199248993786586 60-61 21.05824795197158 27.622525689359247 30.476649103214637 20.84257725545454 62-63 21.06461876316322 27.606598661380144 30.516466673162395 20.812315902294245 64-65 20.94750826331687 27.598073016991325 30.460253633236146 20.994165086455656 66-67 20.886610803396767 27.70394090885243 30.28336993426822 21.126078353482587 68-69 21.085417587935932 27.627116420953225 30.24842416111407 21.039041829996776 70-71 21.021053657220378 27.765400499171793 29.945904317910976 21.267641525696853 72-73 20.961788177626044 27.719919877530298 29.863889237618586 21.454402707225075 74-75 20.935161008350455 28.115071948074092 29.533234585667344 21.416532457908104 76-77 20.982735101670652 27.818896575501604 29.814459492883426 21.38390882994431 78-79 20.968213399689702 27.936100763747834 29.654627082693132 21.44105875386933 80-81 20.787582164726693 28.319942138044237 29.584641847984948 21.307833849244123 82-83 20.890639404591482 28.080849341557922 29.736604432585573 21.29190682126502 84-85 21.00160582218283 28.00801224697058 29.425895147493925 21.564486783352667 86-87 20.863750833826757 28.041406525209673 29.213448407671954 21.88139423329161 88-89 20.82524490147728 27.697289032461157 29.500978106894713 21.97648795916685 90-91 20.773825418464437 27.7526831850613 29.620301790976256 21.85318960549801 92-93 20.660572428647853 27.692212560676143 29.61483004121434 22.032384969461667 94-95 20.447399584773013 27.818334445102344 29.540420923242966 22.193845046881673 96-97 20.605170850166015 27.70431566245194 29.470248311735038 22.22026517564701 98-99 20.477492746168842 27.712024074193785 29.24767207530306 22.56281110433431 100-101 20.284037618071814 27.620067594094373 29.613315680536857 22.482579107296957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 242.0 2 198.5 3 294.5 4 247.0 5 207.0 6 367.0 7 777.5 8 1301.0 9 1552.0 10 1402.5 11 1226.0 12 1264.5 13 1380.5 14 1453.0 15 1493.0 16 1599.0 17 1690.5 18 1706.0 19 1785.5 20 1968.0 21 2204.5 22 2420.5 23 2761.5 24 3201.5 25 3788.5 26 4547.0 27 5467.0 28 6151.0 29 6825.5 30 7857.5 31 8851.0 32 10035.0 33 11031.5 34 11857.0 35 12794.5 36 14130.0 37 15248.5 38 15656.5 39 16038.5 40 16586.0 41 17369.0 42 17894.5 43 18404.0 44 19130.0 45 19514.5 46 19364.5 47 19589.0 48 21482.0 49 20858.5 50 18245.5 51 17061.0 52 15758.0 53 14840.0 54 14191.0 55 13002.0 56 11499.5 57 10337.5 58 9443.0 59 8234.0 60 6684.5 61 5405.5 62 4345.5 63 3172.0 64 2183.5 65 1559.5 66 1122.5 67 833.0 68 611.0 69 393.5 70 284.0 71 232.5 72 171.0 73 157.5 74 149.5 75 122.5 76 94.0 77 55.5 78 36.5 79 26.0 80 16.5 81 10.5 82 3.5 83 1.0 84 1.0 85 1.5 86 0.5 87 1.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041972403144932204 2 0.0 3 0.0 4 1.8737679975416163E-4 5 0.009556216787462244 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0044970431940998794 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.368839987708082E-5 22-23 4.6844199938540407E-4 24-25 2.8106519963124247E-4 26-27 0.001592702797910374 28-29 0.0026232751965582627 30-31 9.368839987708082E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011055231185495536 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.368839987708082E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.8737679975416163E-4 74-75 9.368839987708082E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.8737679975416163E-4 86-87 0.0 88-89 0.0 90-91 0.006839253191026899 92-93 0.001967456397418697 94-95 0.0 96-97 0.0 98-99 9.368839987708082E-5 100-101 0.010774165985864295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 533684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.09014197608704 #Duplication Level Percentage of deduplicated Percentage of total 1 84.697656448398 58.51773109061662 2 9.760485272755561 13.487066265003767 3 2.529342507623356 5.24257898873549 4 1.019825563182013 2.8183957180435275 5 0.5367932252043939 1.8543560070586618 6 0.33509814857989567 1.389118719678534 7 0.21643432400541965 1.0467434721823008 8 0.14954242907636983 0.8265526125068253 9 0.11355128304618056 0.7060746840504714 >10 0.5850663151119957 7.1542778926398345 >50 0.03574168833293026 1.6865424397893154 >100 0.01855293384675616 2.4799540456060627 >500 8.185117873567658E-4 0.3347824859376719 >1k 0.001091349049809021 2.455825578150895 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4891 0.9164599275976046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4105 0.7691817629908335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2937 0.5503256608779727 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.20517759573080702 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.14146948381439203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.8737679975416163E-4 0.0 0.0 0.0 0.0 7 3.7475359950832327E-4 0.0 0.0 0.0 0.0 8 3.7475359950832327E-4 0.0 0.0 0.0 0.0 9 3.7475359950832327E-4 0.0 0.0 0.0 0.0 10-11 3.7475359950832327E-4 0.0 0.0 0.0 0.0 12-13 3.7475359950832327E-4 0.0 0.0 0.0 0.0 14-15 3.7475359950832327E-4 0.0 0.0 0.0 0.0 16-17 3.7475359950832327E-4 0.0 0.0 1.8737679975416163E-4 0.0 18-19 4.6844199938540407E-4 0.0 0.0 2.8106519963124247E-4 0.0 20-21 5.621303992624849E-4 0.0 0.0 5.621303992624849E-4 0.0 22-23 5.621303992624849E-4 0.0 0.0 5.621303992624849E-4 0.0 24-25 7.495071990166465E-4 0.0 0.0 0.0011242607985249699 0.0 26-27 7.495071990166465E-4 0.0 0.0 0.0017800795976645354 0.0 28-29 8.431955988937273E-4 0.0 0.0 0.0022485215970499397 0.0 30-31 9.368839987708081E-4 0.0 0.0 0.003185405595820748 0.0 32-33 9.368839987708081E-4 0.0 0.0 0.005340238792993607 0.0 34-35 9.368839987708081E-4 0.0 0.0 0.0073076951904123034 0.0 36-37 9.368839987708081E-4 0.0 0.0 0.008619332788691435 0.0 38-39 0.0011242607985249699 0.0 0.0 0.011617361584758021 0.0 40-41 0.0011242607985249699 0.0 0.0 0.013772194781930881 0.0 42-43 0.0011242607985249699 0.0 0.0 0.019674563974186973 0.0 44-45 0.0011242607985249699 0.0 0.0 0.033727823955749095 0.0 46-47 0.0013116375982791316 0.0 0.0 0.046656823138786246 0.0 48-49 0.001499014398033293 0.0 0.0 0.07560653870080422 0.0 50-51 0.001499014398033293 0.0 0.0 0.10633633386048674 0.0 52-53 0.001499014398033293 0.0 0.0 0.13556711462213594 0.0 54-55 0.001499014398033293 0.0 0.0 0.17697738736780566 0.0 56-57 0.001499014398033293 0.0 0.0 0.22972395649860217 0.0 58-59 0.001499014398033293 0.0 0.0 0.29708591601022327 0.0 60-61 0.001499014398033293 0.0 0.0 0.3696007375150838 0.0 62-63 0.001499014398033293 0.0 0.0 0.4966422077484054 0.0 64-65 0.0016863911977874548 0.0 0.0 0.5941718320204465 0.0 66-67 0.0016863911977874548 0.0 0.0 0.7366718882334865 0.0 68-69 0.0016863911977874548 0.0 0.0 0.9238613111878939 0.0 70-71 0.0016863911977874548 0.0 0.0 1.0487479482240427 0.0 72-73 0.0016863911977874548 0.0 0.0 1.2267559079904964 0.0 74-75 0.0016863911977874548 0.0 0.0 1.480464094857631 0.0 76-77 0.0016863911977874548 0.0 0.0 1.6800203865958132 0.0 78-79 0.0016863911977874548 0.0 0.0 1.8598084259599315 0.0 80-81 0.0023422099969270206 0.0 0.0 2.1233538948141595 0.0 82-83 0.0024358983968041014 0.0 0.0 2.463349097968086 0.0 84-85 0.002623275196558263 0.0 0.0 2.799128323127544 0.0 86-87 0.0028106519963124244 0.0 0.0 3.23187504215978 0.0 88-89 0.0028106519963124244 0.0 0.0 3.7171809535230587 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCGAC 125 1.3460522E-10 37.998764 5 GGTATCA 3080 0.0 37.635696 1 GTATCAA 4815 0.0 34.138206 1 GGCGACA 160 5.2750693E-11 32.65519 6 GCGACAG 155 1.3533281E-9 30.644165 7 GCGTAGA 65 0.0058368836 29.235296 1 AGGACCG 115 3.8604467E-6 28.912102 5 AAGGCGA 170 3.6325218E-9 27.940268 4 TATCAAC 6025 0.0 27.277122 2 TGAGCCG 70 0.008388944 27.141973 5 ATCAACG 6110 0.0 26.897652 3 TCAACGC 6120 0.0 26.77609 4 GTACATG 3185 0.0 26.401264 1 CAACGCA 6205 0.0 26.256195 5 AACGCAG 6325 0.0 25.83315 6 TACATGG 3215 0.0 25.706783 2 CGACAGC 205 9.549694E-10 25.486975 8 GTAAGGT 225 1.0186341E-10 25.332508 4 GAGTACT 4350 0.0 24.51357 12-13 ACATGGG 3205 0.0 24.453184 3 >>END_MODULE