##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937655_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 449328 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923358882598013 33.0 31.0 34.0 30.0 34.0 2 32.05249172096998 33.0 31.0 34.0 30.0 34.0 3 32.04269264323612 34.0 31.0 34.0 30.0 34.0 4 35.66118292205249 37.0 35.0 37.0 35.0 37.0 5 35.68746884236015 37.0 35.0 37.0 35.0 37.0 6 35.78924749848663 37.0 35.0 37.0 35.0 37.0 7 35.75255715201367 37.0 35.0 37.0 35.0 37.0 8 35.745359737207565 37.0 35.0 37.0 35.0 37.0 9 37.38754540113236 39.0 37.0 39.0 35.0 39.0 10-11 37.287309048178614 39.0 37.0 39.0 34.0 39.0 12-13 37.21859198625503 39.0 37.0 39.0 34.0 39.0 14-15 38.35106425595556 40.0 38.0 41.0 34.0 41.0 16-17 38.29672644482427 40.0 38.0 41.0 34.0 41.0 18-19 38.36799620767012 40.0 38.0 41.0 34.0 41.0 20-21 38.33967280027063 40.0 38.0 41.0 34.0 41.0 22-23 38.24156184346402 40.0 38.0 41.0 34.0 41.0 24-25 38.268946070576504 40.0 38.0 41.0 34.0 41.0 26-27 38.19316178827049 40.0 38.0 41.0 34.0 41.0 28-29 38.0684344176192 40.0 38.0 41.0 34.0 41.0 30-31 37.798523572980095 40.0 37.0 41.0 33.0 41.0 32-33 37.54105464159812 40.0 36.0 41.0 33.0 41.0 34-35 37.45948171491649 40.0 36.0 41.0 33.0 41.0 36-37 37.309023697610655 40.0 36.0 41.0 33.0 41.0 38-39 37.126413221521915 40.0 36.0 41.0 33.0 41.0 40-41 36.9067474094648 40.0 35.5 41.0 31.5 41.0 42-43 36.57764817683296 39.0 35.0 41.0 31.0 41.0 44-45 36.20776136808745 39.0 35.0 41.0 30.0 41.0 46-47 35.6854825428195 39.0 35.0 41.0 28.0 41.0 48-49 35.2626277463234 39.0 35.0 41.0 25.5 41.0 50-51 34.35176752839796 38.0 34.0 40.0 23.5 40.5 52-53 34.447936696578 38.5 35.0 40.0 24.0 40.5 54-55 34.77187043763131 39.0 35.0 40.0 23.0 41.0 56-57 34.59593659865399 39.0 35.0 40.0 22.5 41.0 58-59 34.40420361072535 39.0 35.0 40.0 22.0 41.0 60-61 34.15682641633729 38.0 34.5 40.0 19.5 41.0 62-63 33.82083244311505 38.0 34.0 40.0 18.0 41.0 64-65 33.47437506676637 38.0 34.0 40.0 13.5 41.0 66-67 33.063459210198346 37.0 33.0 40.0 8.0 41.0 68-69 32.57102161449987 36.5 33.0 39.0 7.5 41.0 70-71 32.1483281700673 36.0 32.0 39.0 2.0 40.0 72-73 31.702551365594843 35.5 31.5 38.5 2.0 40.0 74-75 31.169092511483818 35.0 31.0 37.5 2.0 39.5 76-77 30.687261866609692 35.0 31.0 37.0 2.0 39.0 78-79 30.150156455862977 35.0 30.5 36.5 2.0 39.0 80-81 29.60240959833351 34.0 29.5 36.0 2.0 38.5 82-83 29.100151782216997 34.0 29.0 35.5 2.0 37.0 84-85 28.590274810383505 34.0 29.0 35.0 2.0 37.0 86-87 28.110403758501583 34.0 28.5 35.0 2.0 36.0 88-89 27.665024213937258 34.0 27.0 35.0 2.0 36.0 90-91 27.168107707509883 33.5 26.0 35.0 2.0 35.5 92-93 26.62241725421073 33.0 25.0 35.0 2.0 35.0 94-95 25.98353541288324 33.0 24.5 35.0 2.0 35.0 96-97 25.247112354449314 32.0 19.0 34.5 2.0 35.0 98-99 24.353722670298758 32.0 6.0 34.0 2.0 35.0 100-101 21.595164334294772 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04292846572253595 1101 2 0.17391755266142894 1101 3 0.1582634453949936 1101 4 0.12213921451071741 1101 5 0.0502546276775675 1101 6 0.1558561544495305 1101 7 0.18762861028573496 1101 8 0.08304477914322206 1101 9 0.1473616605310042 1101 10-11 0.39927803355617897 1101 12-13 0.1204507601251592 1101 14-15 0.4745452758856814 1101 16-17 0.43233987387596073 1101 18-19 0.4962146863507897 1101 20-21 0.43546120471489047 1101 22-23 0.4348325606099692 1101 24-25 0.28401783952027415 1101 26-27 0.3269711679545182 1101 28-29 0.5622034688137063 1101 30-31 0.4485805148489348 1101 32-33 0.28054281060394004 1101 34-35 0.09124867313961005 1101 36-37 0.09088539346683433 1101 38-39 0.2501711412110268 1101 40-41 0.1178038494359015 1101 42-43 0.19835766173472535 1101 44-45 0.41996958323171896 1101 46-47 0.19767410695289556 1101 48-49 0.10231022501479714 1101 50-51 -0.3239683211241342 1101 52-53 -0.47622009638403995 1101 54-55 -0.5929937350289691 1101 56-57 -0.5950325954649571 1101 58-59 -0.4795318465228675 1101 60-61 -0.4342042914919162 1101 62-63 -0.6241965662490188 1101 64-65 -0.5908547535874789 1101 66-67 -0.5589180411601902 1101 68-69 -1.0197122530057499 1101 70-71 -0.539457565832592 1101 72-73 -0.5271650254548526 1101 74-75 -0.6410408574566873 1101 76-77 -0.5636380053855206 1101 78-79 -0.44114156461728626 1101 80-81 -0.43067676053680515 1101 82-83 -0.40850257020154856 1101 84-85 -0.4504118677616624 1101 86-87 -0.2072426133576144 1101 88-89 -0.3071382928901478 1101 90-91 -0.367312074866323 1101 92-93 -0.5507427785428085 1101 94-95 -0.13880431719893593 1101 96-97 -0.21902637839318473 1101 98-99 -0.0737388206844436 1101 100-101 0.7027485379639415 1102 1 -0.03222364974371317 1102 2 0.10125588575893119 1102 3 0.06761677778497699 1102 4 -0.14340486359881055 1102 5 -0.038962862063485204 1102 6 0.04396992266801192 1102 7 -0.10184358442206332 1102 8 0.07986096705888457 1102 9 0.2033401521115934 1102 10-11 0.11006054381512342 1102 12-13 0.22497884842288585 1102 14-15 0.44974691731768957 1102 16-17 0.3369599389672331 1102 18-19 0.23466806119160566 1102 20-21 0.13086236503751536 1102 22-23 0.047914490707604784 1102 24-25 0.4034956943384458 1102 26-27 0.059608779387936295 1102 28-29 0.20560944023014827 1102 30-31 0.33090682021188655 1102 32-33 0.19561992960776564 1102 34-35 0.4308765208826415 1102 36-37 0.6123089112254263 1102 38-39 0.4020248273579128 1102 40-41 0.32219751011228936 1102 42-43 0.4779529638431228 1102 44-45 0.4621232552283203 1102 46-47 0.43126686201161846 1102 48-49 0.4893839479523905 1102 50-51 0.5147326835454535 1102 52-53 0.6512526643347911 1102 54-55 0.6584071422881408 1102 56-57 0.7105284629756738 1102 58-59 0.5626147503357686 1102 60-61 0.9334623280071668 1102 62-63 0.9620210649947936 1102 64-65 0.7566477227108024 1102 66-67 0.8841553987718882 1102 68-69 0.9401575644556885 1102 70-71 0.9005594144985238 1102 72-73 0.7176418232787007 1102 74-75 0.8947876411990379 1102 76-77 1.0598373023829808 1102 78-79 0.9372934148648113 1102 80-81 0.9064323380906174 1102 82-83 1.0825473802724872 1102 84-85 1.1619979242292828 1102 86-87 1.416855306551824 1102 88-89 1.3742257937095452 1102 90-91 1.671686085597809 1102 92-93 1.3406821542433782 1102 94-95 1.1210088991587845 1102 96-97 1.8400722490300474 1102 98-99 2.0266078610758527 1102 100-101 2.250151962330719 1103 1 -0.04016408974853647 1103 2 0.059277336998199814 1103 3 0.12604529373950157 1103 4 0.23590848249993002 1103 5 0.2576922443223424 1103 6 -0.14988795282910417 1103 7 0.020717709615240665 1103 8 0.05010775004250689 1103 9 0.1893606928113698 1103 10-11 0.315283425137018 1103 12-13 0.3106733356418587 1103 14-15 0.2918017907125332 1103 16-17 0.3437555094603937 1103 18-19 0.27059085279856987 1103 20-21 -0.0872352769330007 1103 22-23 0.288976474344679 1103 24-25 0.00907140971450815 1103 26-27 0.06199225041805079 1103 28-29 -0.2830858102351641 1103 30-31 -0.17716082751645246 1103 32-33 -0.18466421227571317 1103 34-35 0.15561239747776057 1103 36-37 -0.27977542080012086 1103 38-39 -0.021851392831962357 1103 40-41 -0.03753588927979479 1103 42-43 -0.017986431719336338 1103 44-45 0.23117093803921307 1103 46-47 0.36815174571697895 1103 48-49 0.21070864901424358 1103 50-51 0.17838876742220577 1103 52-53 0.05022629886030927 1103 54-55 0.14789241966793298 1103 56-57 0.023938165577405357 1103 58-59 -0.03822749869678432 1103 60-61 -0.037055608281853836 1103 62-63 0.23676631479058585 1103 64-65 0.365169301157799 1103 66-67 0.21012184271344836 1103 68-69 0.3029807725005753 1103 70-71 0.175763281613321 1103 72-73 0.03237046911900521 1103 74-75 0.19451247080763778 1103 76-77 0.5803344837276327 1103 78-79 0.5959013606609318 1103 80-81 0.5226031178070087 1103 82-83 0.6820137773480468 1103 84-85 0.3089128021768168 1103 86-87 0.561835288073226 1103 88-89 0.965858873073774 1103 90-91 0.7268574143159547 1103 92-93 0.5564632766172117 1103 94-95 0.8949172560367025 1103 96-97 1.5504939091577903 1103 98-99 1.9958436774146797 1103 100-101 2.0152528581408973 1104 1 0.3809782802429389 1104 2 0.2995799797944443 1104 3 0.32169746765254814 1104 4 0.29398606135756467 1104 5 0.4184978709208096 1104 6 0.32698757999271777 1104 7 0.3673715885581288 1104 8 0.2905169572036357 1104 9 0.3974808974149511 1104 10-11 0.38247225719157285 1104 12-13 0.3731733356418587 1104 14-15 0.5576798810791104 1104 16-17 0.5370099851637207 1104 18-19 0.762225550155776 1104 20-21 0.5589177494949666 1104 22-23 0.6142641981298453 1104 24-25 0.486906021820225 1104 26-27 0.33921944564396966 1104 28-29 0.7706759118449682 1104 30-31 0.7614580983147547 1104 32-33 0.5477526675196813 1104 34-35 0.6245488851162904 1104 36-37 0.36968110093901174 1104 38-39 0.6760919149259195 1104 40-41 1.0299726358693917 1104 42-43 0.7593473278714526 1104 44-45 0.6478908868883195 1104 46-47 0.40209249593010554 1104 48-49 0.5050394248028169 1104 50-51 0.6283567981127405 1104 52-53 0.3402412178713874 1104 54-55 0.43321850662445627 1104 56-57 0.34768070607186985 1104 58-59 0.09252697700653556 1104 60-61 0.19386937040527386 1104 62-63 0.4525058714828276 1104 64-65 0.32057211445702904 1104 66-67 0.3678903849129327 1104 68-69 0.34374164206578683 1104 70-71 0.4640731707863779 1104 72-73 0.42394122785728783 1104 74-75 0.5987643889662095 1104 76-77 0.5238553703431492 1104 78-79 0.5885484194844608 1104 80-81 0.6535707222400902 1104 82-83 0.6828130100846224 1104 84-85 0.8559076870873028 1104 86-87 0.984629405720284 1104 88-89 0.9773145422979859 1104 90-91 0.9905509352025703 1104 92-93 0.6473626798567658 1104 94-95 1.1233379721492334 1104 96-97 1.6475740455601802 1104 98-99 1.82161094084179 1104 100-101 1.6615124489337347 1105 1 0.1462595620558922 1105 2 0.16992145724321261 1105 3 0.0454393346024915 1105 4 0.03768433073303612 1105 5 0.03164685511945464 1105 6 -0.02793983115232379 1105 7 0.09635291020642 1105 8 0.265553472938592 1105 9 0.22580714890877118 1105 10-11 0.26137003526442726 1105 12-13 0.2688462210488396 1105 14-15 0.40982988329218273 1105 16-17 0.3728468594168035 1105 18-19 0.4740195792798403 1105 20-21 0.36792109123798156 1105 22-23 0.3287194497564485 1105 24-25 0.22481156778579248 1105 26-27 0.3160291875994474 1105 28-29 0.3738134987081594 1105 30-31 0.5468783612280461 1105 32-33 0.6101779762436266 1105 34-35 0.4859744372784647 1105 36-37 0.7877281730409891 1105 38-39 0.739277968128242 1105 40-41 1.0036619422917354 1105 42-43 0.5856403168503377 1105 44-45 0.687369375800813 1105 46-47 0.7224987058181256 1105 48-49 0.8298192238556155 1105 50-51 0.775921862513222 1105 52-53 0.7386417180263081 1105 54-55 0.9250771575340266 1105 56-57 0.7488115873182721 1105 58-59 0.604328198623854 1105 60-61 0.58249084608358 1105 62-63 0.7061768093839476 1105 64-65 0.7117137291164468 1105 66-67 0.6814028666550698 1105 68-69 0.781440818801947 1105 70-71 0.7660942613896609 1105 72-73 0.6733962581764139 1105 74-75 0.8070617598332888 1105 76-77 0.5263229487966505 1105 78-79 0.6617624680835839 1105 80-81 0.5973752192081747 1105 82-83 0.8979155861037427 1105 84-85 0.8158897611810509 1105 86-87 0.7971957979656707 1105 88-89 1.128060901789862 1105 90-91 1.1858298932869324 1105 92-93 1.2122887631569021 1105 94-95 1.4201030099928147 1105 96-97 1.1080097999973937 1105 98-99 1.4954424373230033 1105 100-101 1.5511104438879286 1106 1 0.07187172769120664 1106 2 0.25827558274694695 1106 3 0.0017001846746893534 1106 4 0.21094141162699742 1106 5 0.2680937138769295 1106 6 0.2975701548240366 1106 7 0.3243803735963908 1106 8 0.2778828042352828 1106 9 0.36044448931821904 1106 10-11 0.2340917155984954 1106 12-13 -0.04552826740120963 1106 14-15 -0.11981525092288337 1106 16-17 0.09767599521669723 1106 18-19 0.21871640284789606 1106 20-21 0.2192418675948602 1106 22-23 0.29836792045017546 1106 24-25 0.31285811166308974 1106 26-27 0.531774238923866 1106 28-29 0.5021011031686129 1106 30-31 0.15529045804848352 1106 32-33 0.060709466867599815 1106 34-35 -0.03692996739469123 1106 36-37 -0.0702812003787443 1106 38-39 0.1333525905587578 1106 40-41 -0.16790865290477797 1106 42-43 -0.21566455910043203 1106 44-45 -0.0048821738371316314 1106 46-47 0.15026133621449134 1106 48-49 0.03453043598788952 1106 50-51 0.053086794037206175 1106 52-53 -0.008502725433409353 1106 54-55 -0.2487455607576976 1106 56-57 -0.37089240446564986 1106 58-59 -0.3325341559916879 1106 60-61 -0.08349896723000683 1106 62-63 0.010698259292453827 1106 64-65 -0.077191436690903 1106 66-67 -0.1141517968558432 1106 68-69 -0.4700149806756855 1106 70-71 -0.7554990114352407 1106 72-73 -0.507915290731674 1106 74-75 -0.4294841506343907 1106 76-77 -0.189781816633257 1106 78-79 -0.10407146911763121 1106 80-81 -0.4048169515361195 1106 82-83 -0.5859259520129214 1106 84-85 -0.3448972307227791 1106 86-87 -0.2288860813059337 1106 88-89 -0.13802587793477272 1106 90-91 -0.3523910337328111 1106 92-93 0.008732285435719689 1106 94-95 0.11882941352948251 1106 96-97 0.29748008187770836 1106 98-99 0.2256151522261156 1106 100-101 0.8876620889283018 1107 1 0.09742520383996478 1107 2 0.3062567884489411 1107 3 0.2281376299243547 1107 4 0.21065272802422896 1107 5 0.0693599398863256 1107 6 0.002908810553911678 1107 7 0.03095340059328322 1107 8 0.007555496757383651 1107 9 0.24256203331758286 1107 10-11 0.09877127679941822 1107 12-13 0.24462363990149072 1107 14-15 0.2081193672183943 1107 16-17 0.5444263137641272 1107 18-19 0.16959539464529172 1107 20-21 0.34323147498515993 1107 22-23 0.2052251514766965 1107 24-25 0.2608875972090061 1107 26-27 0.27319087904491823 1107 28-29 0.40710677729459377 1107 30-31 0.21987594821333545 1107 32-33 0.06746615636350128 1107 34-35 0.4275196423847163 1107 36-37 0.3339305938396251 1107 38-39 0.08705371343842927 1107 40-41 0.05034112809386926 1107 42-43 -0.011824071722870144 1107 44-45 -0.276931628324661 1107 46-47 -0.6823818110340554 1107 48-49 -0.5190900545751376 1107 50-51 -0.6296300173030787 1107 52-53 -0.5031073284436829 1107 54-55 -0.5128387956067826 1107 56-57 -0.4876176374034529 1107 58-59 -0.5868594327297671 1107 60-61 -0.3777795477826871 1107 62-63 -0.3699036957923596 1107 64-65 0.038117753402268306 1107 66-67 -0.0413388240120085 1107 68-69 0.022494177562755624 1107 70-71 0.1933243540182943 1107 72-73 0.21987598106891326 1107 74-75 0.5038100279114417 1107 76-77 0.2761161884634973 1107 78-79 0.4620271213100189 1107 80-81 0.6030720745051319 1107 82-83 0.6478231520724513 1107 84-85 0.29499237268568024 1107 86-87 0.36964461870202925 1107 88-89 0.44832536041833393 1107 90-91 0.7164425849659253 1107 92-93 0.521327351932495 1107 94-95 0.6689135299585658 1107 96-97 1.0045931463039253 1107 98-99 1.0121535371906738 1107 100-101 1.3310104205361704 1108 1 -0.145247209953137 1108 2 0.08168782293170551 1108 3 0.047677859809411416 1108 4 0.0877360613575604 1108 5 -0.01275212907918899 1108 6 0.0566444427378201 1108 7 -0.07306958791246387 1108 8 0.08794342779187048 1108 9 0.21059364251298973 1108 10-11 0.17793794346608394 1108 12-13 0.12709490426930614 1108 14-15 0.27158919480459787 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-0.7519471172516354 2316 80-81 -0.670349701960717 2316 82-83 -0.3336524282307849 2316 84-85 -0.7329284310175268 2316 86-87 -1.0489452108967896 2316 88-89 -0.6964904855497309 2316 90-91 -0.8823557177309773 2316 92-93 -0.7943383196091709 2316 94-95 -0.633972053028149 2316 96-97 -1.5265981525019257 2316 98-99 -1.502748180238548 2316 100-101 -2.0700193661269175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1380.0 3 154.0 4 134.0 5 139.0 6 182.0 7 237.0 8 314.0 9 378.0 10 599.0 11 943.0 12 1642.0 13 2348.0 14 2685.0 15 3966.0 16 5481.0 17 7829.0 18 6015.0 19 3156.0 20 2863.0 21 2761.0 22 3051.0 23 3415.0 24 3835.0 25 4333.0 26 4924.0 27 5594.0 28 6779.0 29 7977.0 30 9840.0 31 12164.0 32 15502.0 33 20418.0 34 27764.0 35 40607.0 36 58047.0 37 92521.0 38 80527.0 39 8823.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90568110273138 23.309355087897906 15.61153456305564 20.173429246315074 2 13.947457687638362 23.159992501606798 42.31503963436406 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34.676332455267115 17.09843519904754 22-23 18.459873161041333 29.849405212459157 34.16781581261991 17.522905813879603 24-25 17.84763911417312 30.781982464061358 34.373226757635045 16.997151664130477 26-27 17.300971717957665 30.825207815211954 34.73994812004847 17.133872346781917 28-29 17.100602601051346 30.571774189052842 35.25433555011735 17.073287659778472 30-31 17.89409602982758 30.316159877264496 34.787844190132994 17.001899902774927 32-33 16.880949168719177 30.876750116237712 35.028081024096046 17.21421969094706 34-35 17.52335015815781 30.873274173281196 34.346390690486786 17.25698497807421 36-37 17.92004423160955 30.252615104737956 33.78233767623532 18.045002987417178 38-39 16.88734229559871 31.63941241839609 34.15109082012419 17.32215446588101 40-41 17.126490465590408 31.007815016736913 34.65805539733302 17.207639120339664 42-43 17.17522702392976 30.806361352088306 33.85861531567084 18.159796308311094 44-45 17.937535187321973 30.992937451573884 33.204791607440484 17.864735753663663 46-47 18.169220255236223 30.919994247318485 32.95205095359615 17.958734543849143 48-49 17.717151244137185 31.25310072722854 32.881811705447355 18.147936323186915 50-51 17.878170630686675 31.145011444428466 32.30985692513348 18.666960999751378 52-53 17.78077639370588 31.918345953541277 32.41806158066711 17.882816072085735 54-55 18.43690010902548 31.26612250540111 31.812476171578012 18.484501213995394 56-57 18.568578275650673 32.253915175834585 31.04252354678705 18.134983001727694 58-59 18.29785241916337 31.347970069631685 31.71535608912232 18.638821422082618 60-61 18.573515987842352 31.839930986287534 31.61657265331644 17.969980372553675 62-63 18.10060212359473 31.705340642339475 32.165014412177115 18.029042821888673 64-65 18.107017901707245 31.257711688997823 32.11796557424009 18.517304835054844 66-67 18.335347014476213 32.079278106456115 30.8554652495107 18.729909629556975 68-69 18.1732450933509 31.128225535866168 31.663952362002412 19.034577008780527 70-71 18.419498014842425 31.330044429186387 32.18429752618825 18.06616002978294 72-73 18.37519003820827 31.10485204884595 31.942357677543615 18.577600235402166 74-75 17.96927857826762 32.295836078108785 31.182321082439124 18.55256426118447 76-77 17.78825307617454 31.759363397117717 31.782102399076173 18.670281127631565 78-79 17.574892850972283 31.513128194267175 31.400322143760835 19.511656810999707 80-81 17.922041443696784 31.791223281232412 30.966406552165758 19.32032872290505 82-83 18.270732253739176 31.901639453894333 31.03738454216447 18.79024375020202 84-85 17.933854638021657 31.263569451069728 31.13227734954906 19.67029856135955 86-87 17.691638989813434 32.042328867886674 31.061565209638232 19.20446693266166 88-89 18.326993400748755 31.268703544157916 31.38483966639286 19.01946338870047 90-91 17.866740678568764 30.493349517863734 32.24812267249845 19.39178713106906 92-93 18.5356991596264 30.86879472147299 31.851719756581442 18.743786362319163 94-95 17.95411658884937 30.31419313922293 32.28669162616043 19.44499864576727 96-97 18.640529548593037 30.260238047205974 31.487031565384964 19.612200838816026 98-99 18.059404042453114 30.504537561108275 31.36467902690436 20.071379369534252 100-101 18.195991116133268 29.77566342046699 32.57298934537759 19.455356118022145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1443.0 1 865.5 2 332.5 3 393.5 4 356.0 5 393.5 6 674.5 7 1242.5 8 1840.5 9 1981.5 10 1686.0 11 1428.5 12 1404.0 13 1377.0 14 1291.0 15 1275.0 16 1292.0 17 1307.5 18 1370.5 19 1502.5 20 1719.0 21 1866.5 22 1993.5 23 2335.0 24 2735.5 25 3495.5 26 5106.0 27 7117.0 28 7716.5 29 8430.5 30 10337.0 31 12384.0 32 15460.0 33 18446.5 34 21370.5 35 24201.0 36 27849.0 37 30585.0 38 27497.5 39 22903.0 40 20210.0 41 17269.0 42 14315.5 43 13931.0 44 14178.0 45 13085.0 46 11187.0 47 9729.5 48 10239.0 49 9264.5 50 7110.5 51 5561.0 52 4467.5 53 3826.0 54 3225.0 55 2827.0 56 2493.5 57 2148.0 58 1799.0 59 1466.0 60 1158.5 61 888.5 62 651.5 63 455.5 64 323.5 65 245.5 66 214.5 67 174.0 68 133.0 69 101.5 70 80.5 71 70.5 72 58.0 73 34.5 74 20.5 75 23.5 76 21.0 77 13.5 78 11.0 79 7.0 80 6.0 81 5.0 82 4.0 83 4.5 84 1.5 85 0.5 86 1.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31647259908129477 2 0.27418723070896983 3 0.27262934871630523 4 0.2746323398497312 5 0.2848698500872414 6 0.1878360574012748 7 0.18961649396432007 8 0.18917138482355875 9 0.1878360574012748 10-11 0.19751718121283338 12-13 0.18661200726418117 14-15 0.18850372111241676 16-17 0.19284353523483957 18-19 0.1870571164049425 20-21 0.17904515187123884 22-23 0.17949026101200016 24-25 0.18260602499732934 26-27 0.17637449702667093 28-29 0.19039543496065234 30-31 0.19673824021650108 32-33 0.19885250863511733 34-35 0.19662696293131074 36-37 0.17359256489691272 38-39 0.16546932307801873 40-41 0.17103318733753517 42-43 0.17960153829719047 44-45 0.1859443435530392 46-47 0.18727967097532316 48-49 0.18794733468646513 50-51 0.190617989531033 52-53 0.2165455969803796 54-55 0.1800466474379518 56-57 0.167583591496635 58-59 0.1676948687818253 60-61 0.16057312252964429 62-63 0.16669337321511235 64-65 0.1645791047964961 66-67 0.16246483637787987 68-69 0.17325873304134173 70-71 0.16669337321511235 72-73 0.1633550546594025 74-75 0.166582095929922 76-77 0.16903019620410925 78-79 0.1714782964782965 80-81 0.16546932307801873 82-83 0.16324377737421217 84-85 0.1580137449702667 86-87 0.15534309012569883 88-89 0.15946034967774098 90-91 0.16724975964106398 92-93 0.15979418153331196 94-95 0.1634663319445928 96-97 0.16012801338888297 98-99 0.16446782751130576 100-101 0.14454919346223694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 449328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.891645089557464 #Duplication Level Percentage of deduplicated Percentage of total 1 79.03111123485525 28.365565954747623 2 10.480361554087626 7.52314834619112 3 3.4259445418293772 3.688883567755398 4 1.5496507638784336 2.2247806091954545 5 0.8872350308976951 1.5922162420001316 6 0.5534135186323037 1.1917752959108308 7 0.4424929097188007 1.1117258929190952 8 0.3141006232802942 0.9018870474548089 9 0.26659087420724564 0.8611546537045189 >10 2.3567148887075318 17.913781750364237 >50 0.40922071343289357 10.29313571617191 >100 0.2682600124476632 18.13908025456049 >500 0.011798472769688888 2.801788733689823 >1k 0.0024838890041450293 2.2178505837998115 >5k 6.209722510362573E-4 1.1732253515347408 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5264 1.1715272584837801 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.914476729694121 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2908 0.6471886906669515 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1905 0.42396645657515225 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1029 0.2290086529216964 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.1971833493572624 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 868 0.19317736709041056 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 858 0.190951821386604 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 858 0.190951821386604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 777 0.17292490118577075 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 769 0.1711444646227255 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 720 0.16023929067407328 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 686 0.15267243528113095 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 659 0.1466634618808532 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 649 0.14443791617704663 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 583 0.12974931453192323 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 571 0.12707865968735535 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 544 0.1210696862870776 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 539 0.1199569134351743 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 538 0.11973435886479364 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 525 0.1168411494498451 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 517 0.11506071288679984 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 516 0.11483815831641918 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 508 0.11305772175337393 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 490 0.10905173948652208 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 478 0.1063810846419542 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 461 0.10259765694548303 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 452 0.10059466581205713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1127728519032867E-4 0.0 20-21 0.0 0.0 0.0 3.33831855570986E-4 0.0 22-23 0.0 0.0 0.0 6.67663711141972E-4 0.0 24-25 0.0 0.0 0.0 0.0011127728519032868 0.0 26-27 0.0 0.0 0.0 0.0013353274222839442 0.0 28-29 0.0 0.0 0.0 0.003227041270519531 0.0 30-31 0.0 0.0 0.0 0.006231527970658405 0.0 32-33 0.0 0.0 0.0 0.009569846526368265 0.0 34-35 0.0 0.0 0.0 0.012240501370936154 0.0 36-37 0.0 0.0 0.0 0.01613520635259766 0.0 38-39 0.0 0.0 0.0 0.02103140690097212 0.0 40-41 0.0 0.0 0.0 0.02815315315315315 0.0 42-43 0.0 0.0 0.0 0.0364989495424278 0.0 44-45 0.0 0.0 0.0 0.060757397713919456 0.0 46-47 0.0 0.0 0.0 0.11094345333475768 0.0 48-49 0.0 0.0 0.0 0.14844389844389844 0.0 50-51 0.0 0.0 0.0 0.19395630808674286 0.0 52-53 0.0 0.0 0.0 0.24625663212619736 0.0 54-55 0.0 0.0 0.0 0.302006552006552 0.0 56-57 0.0 0.0 0.0 0.3764510557988819 0.0 58-59 0.0 0.0 0.0 0.48205319944450375 0.0 60-61 0.0 0.0 0.0 0.6010086173129652 0.0 62-63 0.0 0.0 0.0 0.753347220738525 0.0 64-65 0.0 0.0 0.0 0.8970061959192394 0.0 66-67 0.0 0.0 0.0 1.0812813801944237 0.0 68-69 0.0 0.0 0.0 1.3021667912972261 0.0 70-71 0.0 0.0 0.0 1.472754869494 0.0 72-73 0.0 0.0 0.0 1.7048792864010256 0.0 74-75 0.0 0.0 0.0 1.9204233878146921 0.0 76-77 0.0 0.0 0.0 2.1562199551329986 0.0 78-79 0.0 0.0 0.0 2.431297404123491 0.0 80-81 0.0 0.0 0.0 2.7246243278851976 0.0 82-83 0.0 0.0 0.0 3.04699462308158 0.0 84-85 0.0 0.0 0.0 3.4378004486700138 0.0 86-87 0.0 0.0 0.0 3.9305362674927893 0.0 88-89 0.0 0.0 0.0 4.492486557703949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 40 7.471219E-6 59.464172 1 TATCCGG 20 5.3921284E-4 47.49181 94-95 CATTCCG 210 0.0 45.235332 9 TAGGCAT 245 0.0 38.81208 5 GGCATTC 250 0.0 38.00192 7 CCTTTAG 285 0.0 35.05257 1 GGTATCA 2845 0.0 34.612537 1 GCATTCC 295 0.0 33.811497 8 GTATCAA 4505 0.0 33.790966 1 TTAGGCA 295 0.0 32.233765 4 CATGGGT 550 0.0 31.120232 4 GTATAGG 400 0.0 30.921373 1 AGGGGTC 80 4.4645983E-4 29.715498 5 ATGGGTA 355 0.0 29.464384 5 GTACATG 3145 0.0 27.98314 1 AGGCATT 340 0.0 27.942585 6 TACACTG 85 6.3991535E-4 27.942585 7 TATAGGG 120 5.3374606E-6 27.73137 2 TCAACGC 5440 0.0 27.705334 4 ATCAACG 5470 0.0 27.637218 3 >>END_MODULE