##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937655_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 449328 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14076131467436 33.0 31.0 34.0 31.0 34.0 2 32.41824012747926 34.0 31.0 34.0 31.0 34.0 3 32.48078463839333 34.0 31.0 34.0 31.0 34.0 4 36.143852597656945 37.0 35.0 37.0 35.0 37.0 5 36.08062039312039 37.0 35.0 37.0 35.0 37.0 6 36.13518632268632 37.0 35.0 37.0 35.0 37.0 7 36.07177162340206 37.0 35.0 37.0 35.0 37.0 8 36.06599188120927 37.0 35.0 37.0 35.0 37.0 9 37.7381311647616 39.0 37.0 39.0 35.0 39.0 10-11 37.6641718299327 39.0 37.0 39.0 35.0 39.0 12-13 37.59252706263576 39.0 37.0 39.0 35.0 39.0 14-15 38.74477998255172 40.0 38.0 41.0 34.5 41.0 16-17 38.732263513513516 40.0 38.0 41.0 34.5 41.0 18-19 38.84668549478332 40.0 38.0 41.0 35.0 41.0 20-21 38.82146672364064 40.0 38.0 41.0 35.0 41.0 22-23 38.75159237795107 40.0 38.0 41.0 35.0 41.0 24-25 38.743459121176514 40.0 38.0 41.0 35.0 41.0 26-27 38.5881950379233 40.0 38.0 41.0 34.5 41.0 28-29 38.43897664957447 40.0 38.0 41.0 34.0 41.0 30-31 38.32153571555745 40.0 37.5 41.0 34.0 41.0 32-33 38.09132749350141 40.0 37.0 41.0 34.0 41.0 34-35 37.9032099045686 40.0 37.0 41.0 33.5 41.0 36-37 37.7044820264929 40.0 36.0 41.0 33.0 41.0 38-39 37.528537059787055 40.0 36.0 41.0 33.0 41.0 40-41 37.304610440480005 40.0 36.0 41.0 33.0 41.0 42-43 36.9890358490902 40.0 36.0 41.0 32.5 41.0 44-45 36.528754050493184 40.0 35.5 41.0 31.0 41.0 46-47 35.910886924473886 40.0 35.0 41.0 29.0 41.0 48-49 35.42359033935121 39.5 35.0 41.0 26.5 41.0 50-51 35.147069623971795 39.0 35.0 41.0 24.0 41.0 52-53 34.95230766834028 39.0 35.0 41.0 23.5 41.0 54-55 34.736702364419756 39.0 35.0 40.5 23.0 41.0 56-57 34.4931864918278 39.0 35.0 40.0 21.5 41.0 58-59 34.27761902218424 38.5 35.0 40.0 20.0 41.0 60-61 34.02244685396859 38.0 34.0 40.0 18.5 41.0 62-63 33.6835830395613 38.0 34.0 40.0 14.5 41.0 64-65 33.31578601823168 38.0 33.5 40.0 10.0 41.0 66-67 32.91148114517679 37.0 33.0 39.5 7.5 41.0 68-69 32.44416662215575 36.5 32.5 39.0 4.0 40.0 70-71 32.07800203859986 36.0 32.0 39.0 2.0 40.0 72-73 31.599113565146173 35.5 31.5 38.5 2.0 40.0 74-75 31.114140449738276 35.0 31.0 37.5 2.0 39.5 76-77 29.796938984438984 34.0 29.5 36.0 2.0 38.5 78-79 30.349704447530534 35.0 31.0 36.0 2.0 39.0 80-81 30.119295926361147 35.0 31.0 36.0 2.0 38.5 82-83 29.722559021472065 34.0 30.5 36.0 2.0 37.0 84-85 29.30287006373963 34.0 30.0 35.0 2.0 37.0 86-87 28.951154835665704 34.0 29.5 35.0 2.0 36.5 88-89 28.504273047751308 34.0 29.0 35.0 2.0 36.0 90-91 28.004989673467932 34.0 29.0 35.0 2.0 36.0 92-93 27.614328508350248 34.0 27.5 35.0 2.0 35.0 94-95 27.182944307944307 33.5 26.5 35.0 2.0 35.0 96-97 26.68256485240181 33.0 25.5 35.0 2.0 35.0 98-99 25.935760736032478 33.0 24.5 35.0 2.0 35.0 100-101 23.29819307944308 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2620810771417368 1101 2 0.20377858444586394 1101 3 0.17965415215062563 1101 4 0.08357044579057771 1101 5 -0.018038271691679597 1101 6 -0.06552005643903414 1101 7 0.12111669705688399 1101 8 -0.08575170512722963 1101 9 0.05394874056197807 1101 10-11 0.19973290846246528 1101 12-13 -0.09063345464895178 1101 14-15 0.010696344524383505 1101 16-17 0.3178741792844946 1101 18-19 0.1679632950055634 1101 20-21 0.1563257346385214 1101 22-23 0.21972869798541694 1101 24-25 0.12376869645800781 1101 26-27 -0.13598706902462965 1101 28-29 -0.23105350210209252 1101 30-31 -0.08355203722390314 1101 32-33 -0.061600451204483875 1101 34-35 -0.208687507211053 1101 36-37 -0.24037199569325907 1101 38-39 -0.622016956350457 1101 40-41 -0.4170130163876138 1101 42-43 -0.5688166101745011 1101 44-45 -0.658056451086388 1101 46-47 -0.8039312963460432 1101 48-49 -1.0430912544258746 1101 50-51 -1.2616892889356635 1101 52-53 -1.3374974969585551 1101 54-55 -1.3678144656994178 1101 56-57 -1.6063409726411066 1101 58-59 -1.5029562663550493 1101 60-61 -1.5749092807153318 1101 62-63 -1.8062131014206813 1101 64-65 -1.6378943685878937 1101 66-67 -1.4667742772659125 1101 68-69 -1.072771950407823 1101 70-71 -1.4261292108649002 1101 72-73 -1.3707862714148469 1101 74-75 -1.5835855363058435 1101 76-77 -1.1149311353799867 1101 78-79 -1.2975985361226847 1101 80-81 -1.2927153109863667 1101 82-83 -1.1783771767586906 1101 84-85 -1.1442860999374247 1101 86-87 -1.199738332446369 1101 88-89 -1.0253273303818524 1101 90-91 -1.1645768722880518 1101 92-93 -1.2385388249403704 1101 94-95 -1.3870023733767418 1101 96-97 -1.2775969832223133 1101 98-99 -1.3177210908117765 1101 100-101 -1.2767645353825046 1102 1 0.37195796974113904 1102 2 0.34900286631935984 1102 3 0.1271000273451932 1102 4 -0.161950991877454 1102 5 -0.10105794057522388 1102 6 0.09204326604575641 1102 7 0.20122140909876407 1102 8 0.06650526388366984 1102 9 0.21695991927995806 1102 10-11 0.21638778323251984 1102 12-13 0.3355587060981762 1102 14-15 0.42650220273861095 1102 16-17 0.21264565232822008 1102 18-19 0.3165836431023479 1102 20-21 0.18533380029580115 1102 22-23 0.41509448261821547 1102 24-25 0.2688656258481359 1102 26-27 0.4308163836426928 1102 28-29 0.26219681627912195 1102 30-31 0.5321830696106815 1102 32-33 0.5547572677710377 1102 34-35 0.6518968991848766 1102 36-37 0.4756814923497572 1102 38-39 0.4132950572338032 1102 40-41 0.6878829790842929 1102 42-43 1.0427017144328232 1102 44-45 1.0909884052883072 1102 46-47 0.5860998342609989 1102 48-49 0.48833650841550025 1102 50-51 0.7559773305634252 1102 52-53 0.7647877726042012 1102 54-55 0.8494842466254724 1102 56-57 0.7898101523058259 1102 58-59 0.9517770009436575 1102 60-61 0.864039353783042 1102 62-63 0.55243979231075 1102 64-65 0.6602448701272579 1102 66-67 0.727240156015533 1102 68-69 0.8590095693318105 1102 70-71 0.819066770456736 1102 72-73 0.7339682212977614 1102 74-75 0.5415948797122709 1102 76-77 0.5229350030097137 1102 78-79 0.6632476503779543 1102 80-81 0.6321962497890681 1102 82-83 0.7063829760642939 1102 84-85 0.7748662985343415 1102 86-87 0.4975165140231397 1102 88-89 0.32443919006529853 1102 90-91 0.5195677435319581 1102 92-93 0.7076335254204622 1102 94-95 0.747665802652687 1102 96-97 0.6495715465640188 1102 98-99 0.9857457267911691 1102 100-101 0.7741835331048321 1103 1 0.24725422983406986 1103 2 0.1828896648357201 1103 3 0.13327964465079845 1103 4 0.08131704149113972 1103 5 0.012016335345023776 1103 6 0.0024622481161600263 1103 7 0.1385080014047091 1103 8 0.14259556797708228 1103 9 0.0999154233573023 1103 10-11 0.20838708693761276 1103 12-13 0.1629713010018321 1103 14-15 0.1448810407295369 1103 16-17 0.2188453550638556 1103 18-19 0.09277839143191358 1103 20-21 -0.17707096129014133 1103 22-23 -0.20935818941349282 1103 24-25 -0.13071391914992603 1103 26-27 0.07349658342577214 1103 28-29 -0.025202254434987026 1103 30-31 -0.09859316530850748 1103 32-33 -0.11905534855708311 1103 34-35 -0.258110331569938 1103 36-37 -0.2453419272616557 1103 38-39 -0.21221879501776897 1103 40-41 -0.2101767683749003 1103 42-43 0.013152699288411895 1103 44-45 0.11556627650053741 1103 46-47 -0.043417713709700934 1103 48-49 0.06115288741418112 1103 50-51 -0.09369661595577128 1103 52-53 -0.15846245168309991 1103 54-55 -0.6436733168779583 1103 56-57 -0.3471925659223629 1103 58-59 -0.45630872367170383 1103 60-61 -0.3797375104796288 1103 62-63 -0.5638615427008347 1103 64-65 -0.5280962072552029 1103 66-67 -0.3793097027169452 1103 68-69 -0.17981556691435685 1103 70-71 -0.45320462387381966 1103 72-73 -0.13733773336272392 1103 74-75 -0.36540623168152564 1103 76-77 -0.5013415867520763 1103 78-79 -0.12029501777057305 1103 80-81 -0.27819260413629365 1103 82-83 -0.5857629512800138 1103 84-85 -0.589737817639783 1103 86-87 -0.4564778084953076 1103 88-89 -0.32502318992425927 1103 90-91 -0.03292552784898106 1103 92-93 -0.42583750469429305 1103 94-95 -0.0915402872496962 1103 96-97 0.0953241476272062 1103 98-99 0.32516271370885974 1103 100-101 -0.019261252048018207 1104 1 0.40443666802674016 1104 2 0.334104498595309 1104 3 0.27874000270706745 1104 4 0.07907918982873241 1104 5 0.12348266100572403 1104 6 0.08504963089536233 1104 7 0.09023434411570719 1104 8 0.20227161230785384 1104 9 0.23088302779037662 1104 10-11 0.06569740236813004 1104 12-13 0.2473169958014978 1104 14-15 0.20429067414811897 1104 16-17 0.13599156137246382 1104 18-19 0.2887502584395847 1104 20-21 0.17901386394429863 1104 22-23 0.2954904891883814 1104 24-25 0.397099380082814 1104 26-27 0.26509931147351296 1104 28-29 0.2746698683271589 1104 30-31 0.07127043230445196 1104 32-33 0.21598301461428093 1104 34-35 0.35570041011804676 1104 36-37 0.23291894230356291 1104 38-39 0.17855273098393099 1104 40-41 0.11457813358587998 1104 42-43 0.25446770355098636 1104 44-45 0.12648912390036315 1104 46-47 0.10715773386063177 1104 48-49 -0.16028786279895257 1104 50-51 0.04334515727526167 1104 52-53 0.1538777017695807 1104 54-55 0.22967493546645557 1104 56-57 0.6147639558167626 1104 58-59 0.6026213701049343 1104 60-61 0.48162225081620136 1104 62-63 0.24448244706898947 1104 64-65 0.3504204167857097 1104 66-67 0.36866557435040903 1104 68-69 0.46026329071565186 1104 70-71 0.5422663906189342 1104 72-73 0.6795846877796414 1104 74-75 0.4858831971334787 1104 76-77 0.5814056425744383 1104 78-79 0.7060050674809197 1104 80-81 0.6270183933061588 1104 82-83 0.6739876881061768 1104 84-85 0.7465260357276513 1104 86-87 0.730276240950559 1104 88-89 0.4581716097347339 1104 90-91 0.6128012411194739 1104 92-93 0.7624084458598404 1104 94-95 0.5555718184621519 1104 96-97 0.7274639600739263 1104 98-99 0.7887177009211364 1104 100-101 0.6967979977388552 1105 1 0.31043078124764634 1105 2 0.16591634297188307 1105 3 0.10036063741693368 1105 4 0.12879038356130224 1105 5 0.08277314921003409 1105 6 0.20099815477443883 1105 7 0.21005574897561985 1105 8 0.1873554878445276 1105 9 0.03772032252769009 1105 10-11 0.22069563190825647 1105 12-13 0.24980947134702092 1105 14-15 0.35892097999506944 1105 16-17 0.34780218855787837 1105 18-19 0.3443039697661021 1105 20-21 0.13712312344945587 1105 22-23 0.25988488125672404 1105 24-25 0.045170685354356976 1105 26-27 0.2198972577400511 1105 28-29 0.11051504161091685 1105 30-31 0.15128415336849343 1105 32-33 0.27620078117914204 1105 34-35 0.17783558472964955 1105 36-37 0.24991535712231183 1105 38-39 0.5202348891745245 1105 40-41 0.4848172563230122 1105 42-43 0.432650658448523 1105 44-45 0.7446645543434229 1105 46-47 0.9326955110482587 1105 48-49 0.5023178115249536 1105 50-51 0.6222744716646744 1105 52-53 0.9341027714814416 1105 54-55 0.7161004876286299 1105 56-57 0.94577909324871 1105 58-59 0.4913424341513277 1105 60-61 0.4614002794091192 1105 62-63 0.5281350164488856 1105 64-65 0.5937479961244492 1105 66-67 0.3417822476563046 1105 68-69 0.553010491175975 1105 70-71 0.763023372870073 1105 72-73 0.637901423051634 1105 74-75 0.8732017249960897 1105 76-77 0.7514223512895271 1105 78-79 0.8156739914839335 1105 80-81 0.6340310742250281 1105 82-83 0.668767597812721 1105 84-85 0.9279456125877417 1105 86-87 0.6756658527772323 1105 88-89 0.8756488150638191 1105 90-91 1.0072735334223992 1105 92-93 0.8509950656093253 1105 94-95 0.9161121406858506 1105 96-97 0.7508118997012438 1105 98-99 0.7869583064184127 1105 100-101 0.6198189555576477 1106 1 0.06025222750687931 1106 2 -0.023710336345594385 1106 3 -0.2161825621618405 1106 4 -0.07034004668804528 1106 5 -0.165257546393633 1106 6 -0.10075506049620486 1106 7 -0.007289769455823603 1106 8 -0.046834034569833705 1106 9 0.03297626693360911 1106 10-11 -0.15673048333580653 1106 12-13 -0.3123923960383834 1106 14-15 -0.31286335836557555 1106 16-17 -0.12706995653724107 1106 18-19 -0.1084613975854154 1106 20-21 0.08456055144147001 1106 22-23 0.13585128708722038 1106 24-25 0.033680686970463114 1106 26-27 -0.04655444933796815 1106 28-29 0.06331950832172595 1106 30-31 0.16092084212195346 1106 32-33 -0.12833161654997127 1106 34-35 -0.6901493385574042 1106 36-37 -0.22612194887970105 1106 38-39 0.028544806336007866 1106 40-41 0.15716462545746168 1106 42-43 -0.30980968176803714 1106 44-45 -0.4803209788936371 1106 46-47 -0.7206000436152564 1106 48-49 -0.49625897179682 1106 50-51 -0.5479354658284805 1106 52-53 -0.32708616183590067 1106 54-55 -0.4292057646195815 1106 56-57 -0.4551144574423063 1106 58-59 -0.547488442873366 1106 60-61 -0.4434162403308406 1106 62-63 -0.6693115347269583 1106 64-65 -0.9998037375411428 1106 66-67 -1.1201323697695003 1106 68-69 -0.4943935720294448 1106 70-71 -0.5572134128497979 1106 72-73 -0.5501508553983676 1106 74-75 -0.8620512999910055 1106 76-77 -0.6155303489122268 1106 78-79 -0.5805615222034533 1106 80-81 -0.7914794330761268 1106 82-83 -0.8443437206698015 1106 84-85 -0.844089482997159 1106 86-87 -0.5107207929669357 1106 88-89 -0.37069278821844165 1106 90-91 -0.7155537151817519 1106 92-93 -1.0149853638580382 1106 94-95 -0.36142032481548014 1106 96-97 -0.1045335040387414 1106 98-99 -0.2574377127454852 1106 100-101 -0.7627891281045578 1107 1 0.022041468567643108 1107 2 0.08321707466021877 1107 3 -0.023491234615448775 1107 4 0.06450008232366145 1107 5 0.09707123436609777 1107 6 -0.010242796420392608 1107 7 0.16680635222929396 1107 8 0.07431353252421502 1107 9 0.12891379182013196 1107 10-11 0.02876366335528502 1107 12-13 0.07095205327275522 1107 14-15 0.020889727562597216 1107 16-17 0.03886089876259291 1107 18-19 0.08223403125905548 1107 20-21 0.30855662932631134 1107 22-23 0.016099853623813942 1107 24-25 0.015316925721755581 1107 26-27 0.026868243184125618 1107 28-29 -0.028892504897989113 1107 30-31 0.2098775993091806 1107 32-33 -0.0051757074952547555 1107 34-35 -0.13992585763043053 1107 36-37 -0.07926412057675947 1107 38-39 0.13821720021989847 1107 40-41 -0.001315206508778033 1107 42-43 -0.15782523086415523 1107 44-45 -0.35764289097455304 1107 46-47 -0.6817621444355169 1107 48-49 -0.7039195396076039 1107 50-51 -0.8850059245367774 1107 52-53 -0.5717181400154061 1107 54-55 -0.8272681003685634 1107 56-57 -0.986909472175121 1107 58-59 -0.8117633493000653 1107 60-61 -1.0018547606780572 1107 62-63 -1.1933344900506881 1107 64-65 -0.961065384431329 1107 66-67 -1.3161510247030073 1107 68-69 -1.10538495133979 1107 70-71 -1.022663460631911 1107 72-73 -0.8651245752359067 1107 74-75 -1.0584457413384634 1107 76-77 -0.8062929713538907 1107 78-79 -1.0389661968868964 1107 80-81 -0.8719674079108763 1107 82-83 -0.6999347256869264 1107 84-85 -0.6220295761722809 1107 86-87 -0.9154303175349057 1107 88-89 -1.0318976938487694 1107 90-91 -0.7990479813281262 1107 92-93 -0.9789919936262947 1107 94-95 -1.213930378968545 1107 96-97 -1.0763097045643448 1107 98-99 -1.2719440637847477 1107 100-101 -0.5421303322138193 1108 1 0.005662158222818903 1108 2 -0.11699844258116343 1108 3 -0.09540215415567843 1108 4 -0.03427865330851887 1108 5 -0.04833106448447211 1108 6 0.05232904266006955 1108 7 -0.05804996960978315 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-0.7001068131620514 2316 94-95 -0.7521406634177588 2316 96-97 -0.9942496280850719 2316 98-99 -0.8535345307108173 2316 100-101 -0.47220043821140223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 10.0 6 18.0 7 47.0 8 123.0 9 255.0 10 465.0 11 857.0 12 1458.0 13 2213.0 14 2849.0 15 4316.0 16 6076.0 17 8683.0 18 7182.0 19 3498.0 20 2564.0 21 2310.0 22 2353.0 23 2546.0 24 3030.0 25 3471.0 26 3811.0 27 4732.0 28 5710.0 29 7088.0 30 8817.0 31 10838.0 32 13806.0 33 18300.0 34 25366.0 35 37548.0 36 56000.0 37 99345.0 38 94859.0 39 8780.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32493939940301 23.31915424048365 16.630495511437875 19.725410848675466 2 14.302024356372184 23.373571199658155 41.8262382936296 20.498166150340065 3 16.869191325713064 28.81547555460599 33.63956842217712 20.67576469750383 4 10.588701349570915 20.314558629776023 41.72764661895096 27.3690934017021 5 9.611831179066133 41.456768465624485 36.57883371253779 12.352566642771592 6 24.882713741409393 42.73292561336039 18.82455578107752 13.559804864152692 7 23.897242103763844 37.73056653491436 22.639141117401987 15.733050243919807 8 24.70533774881601 36.118826336217644 21.398621942100203 17.777213972866146 9 26.387405191753015 16.759471922515402 23.267190115016202 33.58593277071538 10-11 22.32878876900616 29.324346579781363 29.58406776341559 18.76279688779689 12-13 23.53033474549394 28.784707268787912 29.943257628616227 17.741700357101923 14-15 21.044982729765337 29.676650464693942 29.64515899298508 19.63320781255564 16-17 16.88087544065805 32.821012712317064 30.978149592280026 19.319962254744862 18-19 16.733878146921626 30.069348004130614 36.13529537442581 17.06147847452195 20-21 17.833742833742832 28.874007406616105 36.48337072250116 16.808879037139906 22-23 18.068456093535964 28.713435390235787 36.06013026220471 17.15797825402354 24-25 17.760673184562688 29.337765146541383 36.31030407698625 16.591257591909677 26-27 17.02724778053997 29.55081111276793 36.721519804326974 16.700421302365122 28-29 16.725773911727494 29.33548025981223 37.25203054807828 16.686715280381996 30-31 17.64969020403803 28.824600291991597 36.75077003881352 16.77493946515686 32-33 16.804873054873052 29.55112968699925 36.83367161628031 16.81032564184738 34-35 17.435592707331836 29.407693266388918 36.333702773920166 16.82301125235908 36-37 17.772206495032584 28.938325677456113 35.57868639390379 17.71078143360752 38-39 16.674678631200372 30.289565751522275 36.09479044261653 16.94096517466083 40-41 16.986219359663142 29.717955337550016 36.58268161646555 16.713143686321292 42-43 17.1134295968617 29.504715800016694 35.69473889213477 17.687115710986838 44-45 17.888157426200905 29.603875120179467 34.895110030979595 17.612857422640033 46-47 18.14420645942385 29.77980450806538 34.51888152975109 17.557107502759678 48-49 17.616974682192073 30.193422177117828 34.407938076416336 17.78166506427376 50-51 17.915309083787346 30.024391980913723 33.63144518035822 18.42885375494071 52-53 17.926214257736 30.88033685859773 33.48856514617384 17.704883737492434 54-55 18.75148833700178 30.006431841398356 32.98254945729947 18.259530364300385 56-57 18.649961720613895 31.100109496848628 32.24715575259054 18.00277302994694 58-59 18.400255492646796 30.436563045258698 32.71908271908272 18.444098743011786 60-61 18.498735890040237 30.875218103478975 32.701167076167074 17.92487893031371 62-63 18.20540896627853 30.778073033507813 33.1541769041769 17.86234109603675 64-65 18.26249421358117 30.378476302389345 33.101097639141116 18.25793184488837 66-67 18.51598386924474 31.100109496848628 31.69433019976498 18.689576434141653 68-69 18.325477156998897 30.006810169853647 32.665001958480225 19.002710714667238 70-71 18.396026955809564 30.451696756044583 33.212152369761064 17.94012391838479 72-73 18.538461880853184 30.033961827440088 33.02554036249688 18.40203592920984 74-75 18.288866930171277 31.27726293487163 31.843219207349645 18.59065092760745 76-77 17.791680019940888 30.828259089128657 32.87798668233451 18.502074208595946 78-79 17.521610048783963 30.546059893885978 32.45112701634441 19.48120304098565 80-81 17.920872948046863 30.90860128903607 31.947708578143363 19.222817184773707 82-83 18.145996772937185 30.98948422633951 32.006231569576585 18.85828743114672 84-85 17.611967204358507 30.35699978634761 32.26751949578037 19.763513513513516 86-87 17.353803902716948 30.975367660150273 32.046411530107186 19.6244169070256 88-89 17.955257629170674 30.352214863084427 32.40906420254247 19.283463305202435 90-91 17.579191387021993 29.33723294504152 33.300947859386945 19.782627808549545 92-93 17.955243479034984 29.627214457402296 32.97149025193626 19.446051811626457 94-95 17.230619948011253 29.172119253640993 33.502808638678204 20.09445215966955 96-97 18.04617117117117 28.851751949578038 32.62783534522665 20.47424153402414 98-99 17.362139665830224 29.197736592313984 32.404564650112114 21.03555909174368 100-101 17.141381674427716 28.77894811820537 33.56523732782492 20.514432879541992 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 832.0 1 577.0 2 405.5 3 478.0 4 377.5 5 393.0 6 789.5 7 1445.5 8 1989.0 9 2185.0 10 1875.5 11 1463.5 12 1396.5 13 1387.5 14 1397.5 15 1379.5 16 1371.5 17 1487.5 18 1584.0 19 1714.0 20 1935.0 21 2136.0 22 2318.5 23 2612.0 24 2974.0 25 3566.0 26 5187.5 27 7325.0 28 7870.5 29 8570.5 30 10355.0 31 11956.0 32 14792.5 33 17903.5 34 20526.0 35 23414.0 36 27442.0 37 30152.0 38 27221.5 39 22766.0 40 20263.5 41 17424.0 42 14283.5 43 14006.0 44 14410.5 45 13237.5 46 11324.0 47 9934.0 48 10603.0 49 9628.0 50 7295.0 51 5701.5 52 4487.0 53 3684.5 54 3061.0 55 2721.0 56 2455.0 57 2070.0 58 1667.0 59 1354.0 60 1096.5 61 877.5 62 650.0 63 444.0 64 311.0 65 245.0 66 208.0 67 180.0 68 131.0 69 104.5 70 86.0 71 56.5 72 41.0 73 35.0 74 29.5 75 20.0 76 15.0 77 15.0 78 12.5 79 7.5 80 4.5 81 4.0 82 3.0 83 2.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0166915927785493 2 0.0 3 0.0 4 0.0 5 0.03160274899405334 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003672150411280846 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.789409963323007E-4 24-25 2.2255457038065736E-4 26-27 0.0015578819926646015 28-29 7.789409963323007E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.451091407613147E-4 42-43 0.009013460100416622 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.2255457038065736E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.67663711141972E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011684114944984511 92-93 0.001780436563045259 94-95 0.0 96-97 0.0 98-99 0.0012240501370936153 100-101 0.016914147348929955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 449328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.59182887811151 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04418072202648 26.996909444687496 2 10.981240445523326 7.597223807218797 3 3.5581267051783994 3.692463303365097 4 1.6394741211057446 2.2684963298952874 5 0.9086399367199303 1.5715758601416951 6 0.6344882942711696 1.3168866300355893 7 0.45953854679992473 1.1127395141659324 8 0.3619355909067127 1.001601122043454 9 0.2997079699452254 0.9330702128776281 >10 2.388911612136677 17.658131128904515 >50 0.4254730066901131 10.312572759897169 >100 0.27955965386467224 17.757055441124074 >500 0.014203954699821683 3.2772429383590915 >1k 0.003228171522686746 1.9535262356431085 >5k 0.0012912686090746984 2.550505271641054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6276 1.3967524837090053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5144 1.1448207100381014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.8655147242103765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1380 0.3071253071253071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1349 0.30022611544350675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1081 0.2405814905814906 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1048 0.2332371897589289 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 991 0.22055157924723143 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 955 0.21253961471352772 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 906 0.20163444076487558 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 859 0.19117437595698464 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 809 0.1800466474379518 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 741 0.16491293665206708 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 724 0.1611295089555959 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 674 0.15000178043656304 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 652 0.1451055798881886 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 647 0.1439928070362853 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 646 0.14377025246590464 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.13709361535448492 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 600 0.1335327422283944 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 593 0.1319748602357298 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 569 0.12663355054659403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 556 0.12374034113164549 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 551 0.1226275682797422 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 542 0.12062457714631628 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 528 0.11750881316098707 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 508 0.11305772175337393 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 506 0.11261261261261261 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 501 0.11149983976070932 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 493 0.10971940319766406 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 491 0.10927429405690274 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 469 0.10437809350852828 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 453 0.10081722038243779 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1127728519032867E-4 0.0 20-21 0.0 0.0 0.0 3.33831855570986E-4 0.0 22-23 0.0 0.0 0.0 6.67663711141972E-4 0.0 24-25 0.0 0.0 0.0 0.0011127728519032868 0.0 26-27 0.0 0.0 0.0 0.0013353274222839442 0.0 28-29 0.0 0.0 0.0 0.003004486700138874 0.0 30-31 0.0 0.0 0.0 0.0060089734002777475 0.0 32-33 0.0 0.0 0.0 0.00912473738560695 0.0 34-35 0.0 0.0 0.0 0.011572837659794182 0.0 36-37 0.0 0.0 0.0 0.015244988071075029 0.0 38-39 0.0 0.0 0.0 0.02025246590463982 0.0 40-41 0.0 0.0 0.0 0.027374212156820853 0.0 42-43 0.0 0.0 0.0 0.03616511768685682 0.0 44-45 0.0 0.0 0.0 0.059867179432396826 0.0 46-47 0.0 0.0 0.0 0.11038706690880604 0.0 48-49 0.0 0.0 0.0 0.1494453940106114 0.0 50-51 0.0 0.0 0.0 0.19607057650535914 0.0 52-53 0.0 0.0 0.0 0.2491498415411459 0.0 54-55 0.0 0.0 0.0 0.3062350888437845 0.0 56-57 0.0 0.0 0.0 0.38123597906206597 0.0 58-59 0.0 0.0 0.0 0.4875057864188299 0.0 60-61 0.0 0.0 0.0 0.6061273724317202 0.0 62-63 0.0 0.0 0.0 0.7591336395684223 0.0 64-65 0.0 0.0 0.0 0.9032377238898979 0.0 66-67 0.0 0.0 0.0 1.087179076309511 0.0 68-69 0.0 0.0 0.0 1.3089547056938362 0.0 70-71 0.0 0.0 0.0 1.4802104476017521 0.0 72-73 0.0 0.0 0.0 1.7143378556422033 0.0 74-75 0.0 0.0 0.0 1.9329977210411995 0.0 76-77 0.0 0.0 0.0 2.172355161485596 0.0 78-79 0.0 0.0 0.0 2.450993483602179 0.0 80-81 0.0 0.0 0.0 2.747547448634405 0.0 82-83 0.0 0.0 0.0 3.072810953245736 0.0 84-85 0.0 0.0 0.0 3.4663987109639285 0.0 86-87 0.0 0.0 0.0 3.9650322259017914 0.0 88-89 0.0 0.0 0.0 4.537442580920842 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2980 0.0 39.701595 1 CGCGTTG 30 8.2908526E-5 39.58208 62-63 TACTCCC 85 3.6255005E-7 39.12947 5 CTCCCGC 85 3.6350139E-7 39.11641 7 ACTCCCG 85 3.6350139E-7 39.11641 6 CATTCCG 310 0.0 36.77303 9 GGCATTC 340 0.0 33.52835 7 GTATCAA 4635 0.0 33.52146 1 GGTACTC 115 1.03125785E-7 33.053467 3 TAGGCAT 350 0.0 32.581276 5 GTGTACG 60 0.0039425734 31.67624 1 TCTATAC 60 0.0039425734 31.67624 3 TTAGGCA 375 0.0 31.676239 4 CCTTTAG 380 0.0 30.00907 1 TCGAGCA 40 4.493701E-4 29.686562 74-75 CTTGGAC 65 0.0058317115 29.239605 3 GCATTCC 390 0.0 29.229845 8 GTGGTAC 115 3.8486105E-6 28.921783 1 TCCCGCT 115 3.858633E-6 28.912128 8 AGGCATT 395 0.0 28.859846 6 >>END_MODULE