##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937654_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 464166 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856934803497026 33.0 31.0 34.0 30.0 34.0 2 31.964236932476744 33.0 31.0 34.0 30.0 34.0 3 31.98746353675194 33.0 31.0 34.0 30.0 34.0 4 35.6039972768363 37.0 35.0 37.0 33.0 37.0 5 35.61721883981162 37.0 35.0 37.0 33.0 37.0 6 35.739291546558775 37.0 35.0 37.0 35.0 37.0 7 35.6842142681713 37.0 35.0 37.0 33.0 37.0 8 35.68806202953254 37.0 35.0 37.0 33.0 37.0 9 37.30185106190458 39.0 37.0 39.0 34.0 39.0 10-11 37.21687392010617 39.0 37.0 39.0 34.0 39.0 12-13 37.13440988784185 39.0 37.0 39.0 33.5 39.0 14-15 38.260791397905066 40.0 38.0 41.0 33.5 41.0 16-17 38.233166582645 40.0 38.0 41.0 33.5 41.0 18-19 38.32224785960196 40.0 38.0 41.0 34.0 41.0 20-21 38.23362331579651 40.0 38.0 41.0 34.0 41.0 22-23 38.09611324396876 40.0 38.0 41.0 33.5 41.0 24-25 37.967828320040674 40.0 38.0 41.0 33.0 41.0 26-27 38.02715407849778 40.0 38.0 41.0 33.5 41.0 28-29 37.96151700038348 40.0 38.0 41.0 33.0 41.0 30-31 37.71384375417415 40.0 37.0 41.0 33.0 41.0 32-33 37.41474170878522 39.5 36.0 41.0 33.0 41.0 34-35 37.161288202927395 39.0 35.5 41.0 32.5 41.0 36-37 37.07404032178143 39.0 36.0 41.0 32.0 41.0 38-39 36.975867469827605 39.0 35.0 41.0 32.0 41.0 40-41 36.79701012137899 39.0 35.0 41.0 31.0 41.0 42-43 36.49178634367877 39.0 35.0 41.0 31.0 41.0 44-45 36.133273440967244 39.0 35.0 41.0 30.0 41.0 46-47 35.609392975788836 39.0 35.0 41.0 28.0 41.0 48-49 35.191045875828905 39.0 35.0 41.0 25.0 41.0 50-51 34.27710668166131 38.0 34.0 40.0 23.5 40.5 52-53 34.37790574923626 38.5 34.5 40.0 23.0 40.5 54-55 34.725716661711544 39.0 35.0 40.0 23.0 41.0 56-57 34.5076890595175 39.0 35.0 40.0 21.0 41.0 58-59 34.351666214242314 38.5 35.0 40.0 21.0 41.0 60-61 34.12163105440726 38.0 34.0 40.0 19.5 41.0 62-63 33.796535075813395 38.0 34.0 40.0 17.5 41.0 64-65 33.47733246295506 38.0 34.0 40.0 13.5 41.0 66-67 33.091614853306794 37.0 33.0 40.0 9.0 41.0 68-69 32.608143422827176 36.5 33.0 39.0 7.5 41.0 70-71 32.21930085357394 36.0 32.0 39.0 2.0 40.0 72-73 31.759431970458845 36.0 32.0 38.5 2.0 40.0 74-75 31.266559808344425 35.0 31.0 38.0 2.0 40.0 76-77 30.815475497989944 35.0 31.0 37.0 2.0 39.0 78-79 30.295992166595575 35.0 30.5 36.5 2.0 39.0 80-81 29.769318519667536 34.5 30.0 36.0 2.0 38.5 82-83 29.255981696203513 34.0 29.0 36.0 2.0 37.0 84-85 28.764769500566608 34.0 29.0 35.0 2.0 37.0 86-87 28.283357678072072 34.0 29.0 35.0 2.0 36.0 88-89 27.81329524351202 34.0 27.5 35.0 2.0 36.0 90-91 27.329112860485257 34.0 26.0 35.0 2.0 36.0 92-93 26.844617012017252 33.0 25.5 35.0 2.0 35.0 94-95 26.269527496628363 33.0 25.0 35.0 2.0 35.0 96-97 25.58218934605292 32.5 22.0 35.0 2.0 35.0 98-99 24.7833921484986 32.0 10.0 34.5 2.0 35.0 100-101 22.339982894050834 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27591067655018975 1101 2 0.40319654624318346 1101 3 0.5092109013307073 1101 4 0.2772335111989719 1101 5 0.15641049959696574 1101 6 0.2380131705916213 1101 7 0.19633587803355113 1101 8 0.21274721122417617 1101 9 0.2625000022589461 1101 10-11 0.22439421952865501 1101 12-13 0.4248644768350047 1101 14-15 0.5433912362981843 1101 16-17 0.6317699868357849 1101 18-19 0.47239135538904975 1101 20-21 0.6318338723958874 1101 22-23 0.5660703309616153 1101 24-25 0.5860732412928158 1101 26-27 0.5188644728237719 1101 28-29 0.6284464021502387 1101 30-31 0.8169275268429104 1101 32-33 0.6768254769026143 1101 34-35 0.5918897273107362 1101 36-37 0.6163040413402072 1101 38-39 0.43163577974949163 1101 40-41 0.6953265495805283 1101 42-43 0.5264585097389443 1101 44-45 0.6794730077352611 1101 46-47 0.6878573621971569 1101 48-49 1.0440749880856117 1101 50-51 0.8723937257016416 1101 52-53 0.9405771333173618 1101 54-55 0.8353232004813762 1101 56-57 1.0634846736441688 1101 58-59 0.7431417991206644 1101 60-61 1.0679200298688372 1101 62-63 1.1066133917439345 1101 64-65 1.3342290715603227 1101 66-67 1.4291234719988992 1101 68-69 1.2955210987506547 1101 70-71 1.2532328254775038 1101 72-73 1.1071164394624091 1101 74-75 1.1832880790155968 1101 76-77 1.5995217690829797 1101 78-79 1.7441587698271057 1101 80-81 1.577097544207927 1101 82-83 1.800639987827072 1101 84-85 1.620092368806887 1101 86-87 1.9519950999826179 1101 88-89 1.965220259672666 1101 90-91 1.9067134957563603 1101 92-93 2.1411973072389117 1101 94-95 2.018441635973076 1101 96-97 2.0537792724004014 1101 98-99 2.5586938004465587 1101 100-101 2.733060595529519 1102 1 0.307361877367768 1102 2 0.4196758513019496 1102 3 0.46107600097301926 1102 4 0.38170464047848185 1102 5 0.29453007036855183 1102 6 0.48520274647307104 1102 7 0.295339454936979 1102 8 0.3139480288020948 1102 9 0.2130876363928209 1102 10-11 0.41306310046886807 1102 12-13 0.591177711377675 1102 14-15 0.7582609348163274 1102 16-17 0.7578047338976219 1102 18-19 0.5120949833910942 1102 20-21 0.523721966417348 1102 22-23 0.7328179037771392 1102 24-25 0.7144585248901549 1102 26-27 0.7129881314849911 1102 28-29 0.8147519718998524 1102 30-31 0.790905554436165 1102 32-33 0.6405454564631725 1102 34-35 0.955967397213648 1102 36-37 0.9539254005635058 1102 38-39 0.7630869805670741 1102 40-41 0.786537586882524 1102 42-43 0.816085489810483 1102 44-45 0.8965527215829852 1102 46-47 0.8127296156463117 1102 48-49 0.9564153048970567 1102 50-51 0.9312337870199912 1102 52-53 0.955101916148223 1102 54-55 0.8547279015544476 1102 56-57 0.870204142218526 1102 58-59 0.9071428210930748 1102 60-61 0.7525776691125756 1102 62-63 0.7708826814731111 1102 64-65 0.6279056172935924 1102 66-67 0.7042384438946598 1102 68-69 0.8926340267016002 1102 70-71 1.0323334897595657 1102 72-73 0.9409054021604106 1102 74-75 0.6800305419690957 1102 76-77 0.7899663270799167 1102 78-79 0.8796850856165825 1102 80-81 1.1313770536611685 1102 82-83 0.9383763189461334 1102 84-85 1.085843518525845 1102 86-87 1.049938380514046 1102 88-89 0.949443560643541 1102 90-91 1.4438749673966278 1102 92-93 1.2879653195025824 1102 94-95 1.2387916615223844 1102 96-97 1.3211400286599861 1102 98-99 1.657991194416919 1102 100-101 1.83670137222855 1103 1 0.15670389181583744 1103 2 0.2846507742302187 1103 3 0.038170741689899756 1103 4 0.21397584095701916 1103 5 0.15419049460819423 1103 6 0.2856559742833156 1103 7 0.17020716763195765 1103 8 0.050512239909629386 1103 9 0.23620915666153763 1103 10-11 0.32849125120737455 1103 12-13 0.18529725308844291 1103 14-15 0.5565803108803777 1103 16-17 0.2276604832189335 1103 18-19 0.27771063700541276 1103 20-21 0.40125268007859916 1103 22-23 0.3580134589236934 1103 24-25 0.2916107818266127 1103 26-27 0.18082506149925592 1103 28-29 0.6159931719182552 1103 30-31 0.642513707935457 1103 32-33 0.625004573934298 1103 34-35 0.2897570258889388 1103 36-37 0.02175427830703569 1103 38-39 0.44477496657912496 1103 40-41 0.4760075174029268 1103 42-43 0.4864485321884331 1103 44-45 0.3519661481692893 1103 46-47 0.7687067011844348 1103 48-49 0.7932705480756397 1103 50-51 0.48437302228433055 1103 52-53 0.4133508923595173 1103 54-55 0.7069744850910098 1103 56-57 0.7380606277474371 1103 58-59 0.8405850518021225 1103 60-61 0.721231329444791 1103 62-63 0.8667967292345793 1103 64-65 0.8042215883974393 1103 66-67 0.7532192564838098 1103 68-69 0.6641853541759417 1103 70-71 0.7862273378247195 1103 72-73 0.8687340498390625 1103 74-75 0.7796026212954672 1103 76-77 0.9155357376536983 1103 78-79 0.8174812941473881 1103 80-81 0.6019977687028124 1103 82-83 0.510754729657954 1103 84-85 0.863157971201499 1103 86-87 0.8594159031754351 1103 88-89 1.2780164682034716 1103 90-91 1.2559464715608009 1103 92-93 1.2141144935696673 1103 94-95 1.2093433571005363 1103 96-97 1.9115555258301846 1103 98-99 2.2719078189050244 1103 100-101 2.6885108324963447 1104 1 0.1426824446801902 1104 2 0.3626314237595558 1104 3 0.39235424355724646 1104 4 0.2560838628846369 1104 5 0.3629833834194258 1104 6 0.308107761908623 1104 7 0.3331901103886352 1104 8 0.31539091726346413 1104 9 0.4422720129307862 1104 10-11 0.5821494948136419 1104 12-13 0.6015016342446629 1104 14-15 0.6747579595579509 1104 16-17 0.6463164335978533 1104 18-19 0.6849004507079925 1104 20-21 0.6829374377609128 1104 22-23 0.6695265521597662 1104 24-25 0.5698229381155144 1104 26-27 0.7817434444463771 1104 28-29 0.7391121794968285 1104 30-31 0.6866037334885604 1104 32-33 0.5939368036064749 1104 34-35 0.7059450268499106 1104 36-37 0.6421104929531083 1104 38-39 0.6658038612435533 1104 40-41 0.814657134106362 1104 42-43 0.8936547260862611 1104 44-45 0.898409461773582 1104 46-47 0.5904464963227909 1104 48-49 0.8021213135767624 1104 50-51 0.34582933084352874 1104 52-53 0.8344529518960684 1104 54-55 0.7561696714976307 1104 56-57 1.1255450665619477 1104 58-59 0.919603113697427 1104 60-61 0.9804533793716246 1104 62-63 0.9885015799564059 1104 64-65 0.8080709342816448 1104 66-67 0.7892010853872122 1104 68-69 0.8553258525706866 1104 70-71 0.8927368274178349 1104 72-73 0.6201591268259818 1104 74-75 0.6034008609704813 1104 76-77 0.493603748224384 1104 78-79 0.6337173436810986 1104 80-81 0.9125389703539355 1104 82-83 0.7362936380817438 1104 84-85 0.7481908407933062 1104 86-87 1.0502124174698828 1104 88-89 1.0262801675067657 1104 90-91 1.2213569592538143 1104 92-93 1.3926586218156771 1104 94-95 1.4211884222937172 1104 96-97 0.9258445161549496 1104 98-99 1.2562865726997678 1104 100-101 0.8895122084327447 1105 1 0.20364894113055954 1105 2 0.1252212024783681 1105 3 0.25321279796418494 1105 4 0.24363939076275187 1105 5 0.2618396129208165 1105 6 0.07288751862386533 1105 7 0.14358575949395913 1105 8 0.09650728708950851 1105 9 0.04467164758848696 1105 10-11 0.31665358415643396 1105 12-13 0.03419354274301867 1105 14-15 0.23832959571023338 1105 16-17 0.1888705084099982 1105 18-19 0.2825620524018504 1105 20-21 0.29831087571499637 1105 22-23 0.26967393456521194 1105 24-25 0.3772657495905847 1105 26-27 0.06043630781665854 1105 28-29 -0.00440091885497651 1105 30-31 0.053544406880838835 1105 32-33 0.046431925456438705 1105 34-35 0.14143927686028945 1105 36-37 0.2766644017005646 1105 38-39 0.39811278306860487 1105 40-41 0.49185333952836885 1105 42-43 0.6923428150969286 1105 44-45 0.5305398640652754 1105 46-47 0.8835662289586494 1105 48-49 0.1494092697356848 1105 50-51 0.10771852418433525 1105 52-53 0.283974478030494 1105 54-55 0.2542184114818511 1105 56-57 0.23631539910647348 1105 58-59 0.5531346867451248 1105 60-61 0.4241315045013607 1105 62-63 0.2583203618719594 1105 64-65 0.23735851679503384 1105 66-67 0.33609360002165545 1105 68-69 0.4250730190920464 1105 70-71 0.4288610853162851 1105 72-73 0.6150941540190686 1105 74-75 0.4610263435959645 1105 76-77 0.5407972550953097 1105 78-79 0.27045085138234626 1105 80-81 0.3850396968869205 1105 82-83 0.289366872606589 1105 84-85 0.28288279080783596 1105 86-87 0.7114664134013005 1105 88-89 0.6183734128258216 1105 90-91 0.7166708215031612 1105 92-93 0.9529919966651796 1105 94-95 0.944271413118642 1105 96-97 1.1287911263596264 1105 98-99 1.4850925676347977 1105 100-101 1.3263038387727626 1106 1 0.02682702993364572 1106 2 0.2537604560176199 1106 3 0.14617048779687636 1106 4 0.19372787210122766 1106 5 0.1913964018526073 1106 6 0.07682896006530626 1106 7 0.13172121637941814 1106 8 0.10052916611139295 1106 9 0.19328007744691433 1106 10-11 -0.02283322408037236 1106 12-13 -0.08452227128528733 1106 14-15 -0.3508756809950526 1106 16-17 -0.2109344304574421 1106 18-19 -0.2340583626560644 1106 20-21 -0.20207120279208368 1106 22-23 -0.356251097609821 1106 24-25 0.159792696180034 1106 26-27 -0.05433806477020653 1106 28-29 0.23583596981941213 1106 30-31 0.2229660606774999 1106 32-33 0.24655057088758525 1106 34-35 0.04055044159645149 1106 36-37 0.266750469911635 1106 38-39 0.20576641884724012 1106 40-41 0.17882044055797053 1106 42-43 0.274379092445713 1106 44-45 0.2645528949533045 1106 46-47 0.3580876253550471 1106 48-49 0.30384942417584426 1106 50-51 0.025048800889614142 1106 52-53 0.1677622836932997 1106 54-55 0.3161855125114599 1106 56-57 0.3930781886817698 1106 58-59 0.1537620998725373 1106 60-61 -0.15171107915372062 1106 62-63 0.15573228272138806 1106 64-65 0.23808245501897574 1106 66-67 0.1843983780139311 1106 68-69 0.14964468897620975 1106 70-71 0.010716378109080438 1106 72-73 0.21491719134210285 1106 74-75 0.12460621811334249 1106 76-77 0.4298436675792239 1106 78-79 0.32946187133086724 1106 80-81 0.2413480141327362 1106 82-83 0.07399525098496795 1106 84-85 0.5496741357241817 1106 86-87 0.25096126539615327 1106 88-89 0.7632113311012354 1106 90-91 0.4688728002676399 1106 92-93 0.038647965133648654 1106 94-95 0.2382607149204432 1106 96-97 0.22037936638536593 1106 98-99 0.4041120335292625 1106 100-101 0.0919891669580899 1107 1 0.34819301791762314 1107 2 0.3767577857906552 1107 3 0.40699742371142733 1107 4 0.1909324782427504 1107 5 0.2343495059914602 1107 6 0.2456373712802602 1107 7 0.12316814561840772 1107 8 0.37873343847454066 1107 9 0.4972270066859039 1107 10-11 0.43081330462456435 1107 12-13 0.4985363902634461 1107 14-15 0.7228058944388707 1107 16-17 0.768193990770861 1107 18-19 0.8346269677845228 1107 20-21 0.885190980118459 1107 22-23 0.9429394898401142 1107 24-25 0.787807132094585 1107 26-27 0.5760336808906743 1107 28-29 0.6404483696858918 1107 30-31 0.5240967349098113 1107 32-33 0.4678977838381755 1107 34-35 0.660814960955598 1107 36-37 0.7270517048916076 1107 38-39 0.7934041024253418 1107 40-41 0.9321686548436858 1107 42-43 0.6923217660104655 1107 44-45 0.7575226880107166 1107 46-47 0.9937476720840195 1107 48-49 1.0354916137619554 1107 50-51 0.866232879661311 1107 52-53 0.804423665535758 1107 54-55 0.8580851286663247 1107 56-57 0.6956169904174132 1107 58-59 0.6458348635574538 1107 60-61 0.761537836066573 1107 62-63 0.35490409513793963 1107 64-65 0.5065237100256468 1107 66-67 0.8509016323530503 1107 68-69 0.966118737707859 1107 70-71 0.9366071491371173 1107 72-73 0.7543864837319632 1107 74-75 0.9712246259905086 1107 76-77 0.98081788320005 1107 78-79 1.0011804127193855 1107 80-81 1.064935714399759 1107 82-83 1.4212499238821614 1107 84-85 1.4088405242422049 1107 86-87 1.3077870330864059 1107 88-89 1.3262015680838815 1107 90-91 1.540395246863099 1107 92-93 1.8373483156009414 1107 94-95 1.8290171401540896 1107 96-97 2.26634690643877 1107 98-99 2.4304596637028304 1107 100-101 2.843574614221108 1108 1 0.11794931745625092 1108 2 0.02583700815746326 1108 3 0.08831372167270146 1108 4 0.20523706438574152 1108 5 0.12114512096049879 1108 6 0.09133317947458863 1108 7 0.023617690158800997 1108 8 0.1471687080040951 1108 9 0.1571702221123772 1108 10-11 0.27092431057906197 1108 12-13 0.12357089477860228 1108 14-15 0.07223311948717281 1108 16-17 0.1528059628517795 1108 18-19 0.16263674734997124 1108 20-21 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sequence quality scores pass #Quality Count 2 1486.0 3 170.0 4 146.0 5 169.0 6 209.0 7 278.0 8 359.0 9 430.0 10 635.0 11 975.0 12 1672.0 13 2485.0 14 2922.0 15 4091.0 16 5566.0 17 8014.0 18 6103.0 19 3224.0 20 3046.0 21 2892.0 22 3077.0 23 3486.0 24 3925.0 25 4439.0 26 5002.0 27 5822.0 28 6900.0 29 8403.0 30 10083.0 31 12311.0 32 15968.0 33 20996.0 34 28765.0 35 42073.0 36 59001.0 37 93061.0 38 84898.0 39 11084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.85459296708332 23.18216892573916 15.744629344238556 20.218608762938963 2 14.035284814912435 23.127627673042937 42.26270465063021 20.574382861414417 3 16.775112009781683 29.243518122469787 32.919210379645115 21.062159488103415 4 10.23754226394305 20.069998811748565 42.12308124399123 27.569377680317153 5 9.668644959458321 41.79671043108347 36.340165537088126 12.194479072370084 6 24.486367915158947 42.78561743801439 19.394954790013212 13.33305985681345 7 24.164543845257114 37.77665040369587 22.68339018177108 15.375415569275939 8 24.226194331109816 36.43179414113939 21.677352503076225 17.664659024674567 9 26.40241295777324 17.389229779387954 23.292944646195966 32.91541261664285 10-11 22.122775124575753 29.593973728981894 29.67785185632983 18.60539929011253 12-13 23.16749066257907 29.194822858870012 29.610742891685916 18.026943586865002 14-15 20.99813353282618 30.28750503320023 28.81516577445434 19.899195659519254 16-17 17.113039986875396 33.58798235085872 30.086756236346524 19.21222142591937 18-19 16.943817799125696 31.208807814776833 34.681742133952184 17.165632252145286 20-21 18.261183019790387 29.994139619517767 34.63625168769244 17.108425672999402 22-23 18.5190981741567 29.78501331928728 34.23326670091832 17.462621805637706 24-25 18.01480231025703 30.71225729294953 34.37419385478538 16.89874654200806 26-27 17.280270101363566 30.77594776716278 34.8101983125141 17.13358381895955 28-29 17.050361420811274 30.596948865648184 35.19426908362336 17.158420629917178 30-31 17.912207127706083 30.19360201212239 34.86552567237164 17.028665187799888 32-33 16.99339772957895 30.877164953127767 34.9393532452167 17.190084072076584 34-35 17.590190814521204 30.818320871073634 34.37975295688565 17.21173535751951 36-37 17.943423921255423 30.14203380318173 33.8147300710169 18.099812204545945 38-39 16.86649342352337 31.663565844625076 34.091487919744104 17.37845281210745 40-41 17.068456845144958 31.03780568606842 34.6334141230147 17.260323345771923 42-43 17.139474292029764 30.654210045740953 33.88284358061654 18.323472081612742 44-45 17.866563629220614 30.950952160980176 33.152514128074394 18.029970081724812 46-47 18.174469118480342 31.02139715604004 32.82070100644883 17.983432719030787 48-49 17.737349355971137 31.19729348112389 32.8670619070854 18.198295255819577 50-51 17.82602829185624 31.079699702617074 32.35375074885445 18.740521256672228 52-53 17.86946663787519 31.834916864608076 32.43187216583891 17.86374433167782 54-55 18.552989035113757 31.007023182871507 31.956291560847617 18.48369622116712 56-57 18.61588417032582 32.223302552901735 31.082984144318747 18.077829132453697 58-59 18.257897701643103 31.348754993730182 31.699799927049995 18.69354737757672 60-61 18.481232830300247 31.853583058726038 31.662706710776472 18.002477400197243 62-63 18.10321106114904 31.842456536776076 32.225535041971376 17.828797360103508 64-65 18.120182538817556 31.339679100298152 32.156873747581486 18.383264613302803 66-67 18.260834847615058 32.06881816965423 30.896095095453703 18.774251887277014 68-69 18.084316573468378 31.11650181612564 31.802377905207525 18.996803705198456 70-71 18.361451451732304 31.322368094478602 32.157170821615956 18.15900963217314 72-73 18.43315834057224 31.146697169002003 32.00306462142753 18.417079868998226 74-75 17.962601611977156 32.22508105214534 31.232165290255182 18.58015204562232 76-77 17.79278550107162 31.79184014400508 31.827560827683854 18.58781352723945 78-79 17.656879832934916 31.4749321433004 31.46791713658218 19.4002708871825 80-81 17.91945939354436 31.80962226828955 31.054763166329074 19.21615517183701 82-83 18.23091820275876 31.869797651222132 31.178219050239246 18.72106509577986 84-85 17.8799344465339 31.07510489525579 31.27448410091091 19.770476557299403 86-87 17.692521827700354 31.90965943176767 31.20497969365691 19.19283904687507 88-89 18.365409100914583 31.18873844905499 31.331709043510536 19.11414340651989 90-91 17.930643760150648 30.469047606200732 32.17915859560899 19.421150038039634 92-93 18.458995237098407 30.854840484879354 31.962263662319558 18.723900615702686 94-95 17.973953887871584 30.277175607855916 32.399883030965505 19.348987473306998 96-97 18.70340954707713 30.26561144371593 31.452737263297838 19.578241745909107 98-99 18.181847614936324 30.477552341895098 31.336067342974317 20.004532700194257 100-101 18.20456546509219 29.87039051170499 32.5446921828603 19.38035184034252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1455.0 1 885.0 2 351.5 3 387.5 4 355.5 5 407.5 6 750.5 7 1390.5 8 1935.5 9 2049.0 10 1767.5 11 1455.0 12 1398.0 13 1366.0 14 1290.0 15 1243.0 16 1269.0 17 1364.5 18 1433.0 19 1594.0 20 1790.5 21 1950.0 22 2194.5 23 2469.5 24 2809.5 25 3618.5 26 5266.0 27 7347.5 28 7965.5 29 8704.5 30 10685.0 31 12661.0 32 15775.5 33 19068.5 34 22100.5 35 24917.0 36 28869.0 37 31892.0 38 28625.5 39 23749.0 40 20760.0 41 17740.5 42 14688.0 43 14166.0 44 14539.0 45 13573.0 46 11649.0 47 9958.0 48 10437.5 49 9635.0 50 7458.5 51 5742.5 52 4588.0 53 3956.5 54 3340.0 55 2912.0 56 2605.0 57 2200.5 58 1830.5 59 1505.5 60 1204.0 61 941.5 62 700.0 63 508.0 64 344.0 65 267.0 66 240.0 67 198.5 68 138.0 69 106.5 70 78.0 71 57.0 72 49.5 73 39.0 74 30.0 75 21.0 76 17.0 77 15.0 78 10.0 79 10.0 80 8.0 81 3.5 82 4.5 83 3.0 84 1.0 85 0.5 86 1.5 87 1.0 88 0.0 89 1.0 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3259609708595632 2 0.28093397620678806 3 0.271454608911467 4 0.28028765570937986 5 0.28201117703580186 6 0.21414752480793509 7 0.20380639684940302 8 0.2016519951913755 9 0.2154401658027516 10-11 0.21414752480793509 12-13 0.21026960182348559 14-15 0.21360892439342818 16-17 0.19691231154371497 18-19 0.20272919602038925 20-21 0.19077226681833653 22-23 0.1999284738649535 24-25 0.19982075378205214 26-27 0.20089795461106585 28-29 0.21414752480793509 30-31 0.20973100140897868 32-33 0.2137166444763296 34-35 0.20843836041416217 36-37 0.19303438855926544 38-39 0.18269326060073335 40-41 0.17730725645566456 42-43 0.1949733500514902 44-45 0.19475790988568745 46-47 0.19465018980278606 48-49 0.21285488381311857 50-51 0.20649939892193742 52-53 0.2296592167457332 54-55 0.19702003162661635 56-57 0.1789230576991852 58-59 0.17989253844529757 60-61 0.16739700882873798 62-63 0.1754760150463412 64-65 0.17504513471473568 66-67 0.17235213264220128 68-69 0.17601461546084804 70-71 0.17730725645566456 72-73 0.17526057488053842 74-75 0.1904491065696324 76-77 0.1829087007665361 78-79 0.18818698482870352 80-81 0.1797848183623962 82-83 0.1580253616163183 84-85 0.1567327206215018 86-87 0.16459628667330223 88-89 0.16890508998935724 90-91 0.17956937819659347 92-93 0.17084405148158202 94-95 0.1713826518960889 96-97 0.1766609359582563 98-99 0.18657118358518288 100-101 0.16675068833132972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 464166.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.02198360421397 #Duplication Level Percentage of deduplicated Percentage of total 1 79.04411975834304 28.473259859445587 2 10.716038235240063 7.720259072238951 3 3.279274741026714 3.5437794286493185 4 1.5259024298345825 2.1986412923652634 5 0.8735473748352756 1.5733454606910224 6 0.5567499235394654 1.2033141970431602 7 0.45111771968576125 1.1375108571463617 8 0.3399649604820863 0.9796969785994369 9 0.2411481404068569 0.7817970923930239 >10 2.296258381439295 17.712794528965343 >50 0.3906672646531842 9.949680470160207 >100 0.26903313163999953 18.23913832379752 >500 0.013182024267438731 3.135040757279643 >1k 0.00239673168498886 2.161181624966683 >5k 5.99182921247215E-4 1.1905600562584862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5516 1.188367954567978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4113 0.8861054019467173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2986 0.6433043350870163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1864 0.401580469056329 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1050 0.22621217409288918 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 943 0.20316007635199476 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 899 0.19368070905667367 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 864 0.1861403032535774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.1842013417613526 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 831 0.17903077778208656 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 775 0.1669661284971325 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 760 0.1637345260100912 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 699 0.15059267589612338 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 683 0.14714563324327934 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 638 0.13745082578215553 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 630 0.1357273044557335 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 588 0.12667881749201795 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 586 0.12624793716041244 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 567 0.12215457401016017 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 565 0.12172369367855465 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 542 0.11676856986509138 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 524 0.11289064688064185 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 523 0.1126752067148391 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 522 0.11245976654903633 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 521 0.11224432638323359 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 507 0.10922816406199506 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 503 0.10836640339878406 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 496 0.1068583222381648 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 490 0.10556568124334828 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 484 0.10427304024853178 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.540405803096306E-4 0.0 24-25 0.0 0.0 0.0 0.0015080811606192613 0.0 26-27 0.0 0.0 0.0 0.002154401658027516 0.0 28-29 0.0 0.0 0.0 0.0037702029015481532 0.0 30-31 0.0 0.0 0.0 0.007755845968899057 0.0 32-33 0.0 0.0 0.0 0.012495529616559593 0.0 34-35 0.0 0.0 0.0 0.016588892766811872 0.0 36-37 0.0 0.0 0.0 0.019820495253853147 0.0 38-39 0.0 0.0 0.0 0.02563737973052744 0.0 40-41 0.0 0.0 0.0 0.03360866586522925 0.0 42-43 0.0 0.0 0.0 0.04491927456987371 0.0 44-45 0.0 0.0 0.0 0.0672173317304585 0.0 46-47 0.0 0.0 0.0 0.12323177483917391 0.0 48-49 0.0 0.0 0.0 0.16394996617589397 0.0 50-51 0.0 0.0 0.0 0.21382436455923096 0.0 52-53 0.0 0.0 0.0 0.26994652775084776 0.0 54-55 0.0 0.0 0.0 0.32962345367820994 0.0 56-57 0.0 0.0 0.0 0.40222678955373725 0.0 58-59 0.0 0.0 0.0 0.5097314322893103 0.0 60-61 0.0 0.0 0.0 0.6332863673771884 0.0 62-63 0.0 0.0 0.0 0.7692291119987247 0.0 64-65 0.0 0.0 0.0 0.8967696901539535 0.0 66-67 0.0 0.0 0.0 1.0910967197080355 0.0 68-69 0.0 0.0 0.0 1.3163394130548123 0.0 70-71 0.0 0.0 0.0 1.496555111748814 0.0 72-73 0.0 0.0 0.0 1.736232296204375 0.0 74-75 0.0 0.0 0.0 1.9557658251573788 0.0 76-77 0.0 0.0 0.0 2.18133167875286 0.0 78-79 0.0 0.0 0.0 2.4528940077472283 0.0 80-81 0.0 0.0 0.0 2.7361978257778468 0.0 82-83 0.0 0.0 0.0 3.047508865362823 0.0 84-85 0.0 0.0 0.0 3.44424193068859 0.0 86-87 0.0 0.0 0.0 3.9346914681385536 0.0 88-89 0.0 0.0 0.0 4.493543258230892 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 225 0.0 46.444813 9 GGCATTC 240 0.0 43.54201 7 GGTATCA 2520 0.0 39.05611 1 ACTCCCG 75 6.9129637E-6 38.0003 6 TAGGCAT 280 0.0 37.35398 5 GTATCAA 4055 0.0 35.997032 1 CTACACG 55 0.0025670414 34.57559 4 CGGAGGT 55 0.0025779423 34.545727 9 TTAGGCA 350 0.0 32.599842 4 CCTTTAG 310 0.0 32.208965 1 TACACGG 60 0.0039317184 31.69429 5 CATGGGT 495 0.0 31.694288 4 TACTCCC 90 2.3955543E-5 31.694286 5 GTAGATA 150 9.513315E-10 31.66692 6 ACGGAGG 60 0.0039483844 31.666918 8 GCATTCC 330 0.0 31.666916 8 CTAACCG 30 0.003955628 31.658377 82-83 AGGCATT 335 0.0 31.194277 6 GTATAGG 430 0.0 30.960558 1 CACGGAG 65 0.0058402917 29.231003 7 >>END_MODULE