##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 464166 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.170227892607386 33.0 31.0 34.0 31.0 34.0 2 32.364438584471934 34.0 31.0 34.0 31.0 34.0 3 32.43584838182891 34.0 31.0 34.0 31.0 34.0 4 36.100957416096826 37.0 35.0 37.0 35.0 37.0 5 36.03418389110792 37.0 35.0 37.0 35.0 37.0 6 36.085712869964624 37.0 35.0 37.0 35.0 37.0 7 36.03050848187933 37.0 35.0 37.0 35.0 37.0 8 36.02356484533551 37.0 35.0 37.0 35.0 37.0 9 37.66133236816139 39.0 37.0 39.0 35.0 39.0 10-11 37.618637513303426 39.0 37.0 39.0 35.0 39.0 12-13 37.54844495288324 39.0 37.0 39.0 35.0 39.0 14-15 38.692730617925484 40.0 38.0 41.0 34.5 41.0 16-17 38.67368893887101 40.0 38.0 41.0 34.5 41.0 18-19 38.80786722853462 40.0 38.0 41.0 35.0 41.0 20-21 38.76736232296204 40.0 38.0 41.0 35.0 41.0 22-23 38.61950358276996 40.0 38.0 41.0 34.0 41.0 24-25 38.649604882735915 40.0 38.0 41.0 34.0 41.0 26-27 38.50416445840497 40.0 38.0 41.0 34.0 41.0 28-29 38.35988094776438 40.0 38.0 41.0 34.0 41.0 30-31 38.2382714373737 40.0 37.5 41.0 34.0 41.0 32-33 38.01212389533055 40.0 37.0 41.0 34.0 41.0 34-35 37.83073620213458 40.0 36.0 41.0 33.0 41.0 36-37 37.61313624866966 40.0 36.0 41.0 33.0 41.0 38-39 37.420854823489876 40.0 36.0 41.0 33.0 41.0 40-41 37.211066730436954 40.0 36.0 41.0 33.0 41.0 42-43 36.896344195826494 40.0 35.5 41.0 32.5 41.0 44-45 36.40094276616556 40.0 35.0 41.0 30.5 41.0 46-47 35.81116023146892 39.5 35.0 41.0 29.0 41.0 48-49 35.3391351369984 39.0 35.0 41.0 25.5 41.0 50-51 35.06101157775451 39.0 35.0 41.0 24.0 41.0 52-53 34.85043928249807 39.0 35.0 41.0 23.0 41.0 54-55 34.6540741889755 39.0 35.0 40.0 22.5 41.0 56-57 34.42322143371122 39.0 35.0 40.0 20.5 41.0 58-59 34.20840712159012 38.5 34.5 40.0 19.5 41.0 60-61 33.93458051645317 38.0 34.0 40.0 17.5 41.0 62-63 33.60547411917288 38.0 34.0 40.0 13.5 41.0 64-65 33.247751881869846 37.5 33.5 40.0 8.5 41.0 66-67 32.83458611789747 37.0 33.0 39.5 7.5 41.0 68-69 32.38513053519646 36.5 32.5 39.0 2.0 40.0 70-71 32.009357643601646 36.0 32.0 39.0 2.0 40.0 72-73 31.522516728928874 35.5 31.5 38.5 2.0 40.0 74-75 31.013684759331788 35.0 31.0 37.5 2.0 39.5 76-77 29.68753527832715 34.0 29.5 36.0 2.0 38.5 78-79 30.260284036314594 35.0 31.0 36.0 2.0 39.0 80-81 30.018351193323078 34.5 31.0 36.0 2.0 38.5 82-83 29.63376249014361 34.0 30.0 36.0 2.0 37.0 84-85 29.222740355820978 34.0 30.0 35.0 2.0 37.0 86-87 28.816573165634708 34.0 29.0 35.0 2.0 36.0 88-89 28.42247708793837 34.0 29.0 35.0 2.0 36.0 90-91 27.932483206439073 34.0 29.0 35.0 2.0 36.0 92-93 27.506742199988796 34.0 27.0 35.0 2.0 35.0 94-95 27.082598682367944 33.0 26.0 35.0 2.0 35.0 96-97 26.587614129427834 33.0 25.5 35.0 2.0 35.0 98-99 25.85156818896688 33.0 24.5 35.0 2.0 35.0 100-101 23.3657516922825 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4676168790442148 1101 2 0.3433390996739547 1101 3 0.37247369979797185 1101 4 0.2021088794000363 1101 5 0.3013457754664586 1101 6 0.3452024197011099 1101 7 0.34978571924775537 1101 8 0.193011397592592 1101 9 0.4272178039327841 1101 10-11 0.4097713042980473 1101 12-13 0.47159687006034545 1101 14-15 0.6175242716301241 1101 16-17 0.468859309328586 1101 18-19 0.6378797994830379 1101 20-21 0.5983388799781579 1101 22-23 0.5963524489820884 1101 24-25 0.39927277254047766 1101 26-27 0.5121865503364091 1101 28-29 0.6665974652946716 1101 30-31 0.7564625507092515 1101 32-33 0.7352463228232651 1101 34-35 0.617960218505921 1101 36-37 0.6273821090621894 1101 38-39 0.5232268056910954 1101 40-41 0.6210708750521619 1101 42-43 0.673272996873294 1101 44-45 0.5738269776016764 1101 46-47 0.7934737063055337 1101 48-49 0.7958634829750579 1101 50-51 0.8142079985416402 1101 52-53 0.5893825319256294 1101 54-55 0.5714800270180262 1101 56-57 0.5349270869999216 1101 58-59 0.9070035059193913 1101 60-61 1.0470794947590392 1101 62-63 0.8843091428775622 1101 64-65 0.8763251696138497 1101 66-67 0.5380251430659442 1101 68-69 0.8197824611233386 1101 70-71 1.2341004405716873 1101 72-73 1.0550801315160925 1101 74-75 0.9148598346451031 1101 76-77 0.8647480246665644 1101 78-79 1.1391407019686994 1101 80-81 0.7368210403236866 1101 82-83 0.9761839648628161 1101 84-85 1.028115166229771 1101 86-87 1.0188444974255937 1101 88-89 1.1412420020728824 1101 90-91 1.2678594438245732 1101 92-93 1.301059780057681 1101 94-95 1.0343400536272576 1101 96-97 1.3787216380601066 1101 98-99 1.2382878550964804 1101 100-101 1.639898835617334 1102 1 0.3928595974908191 1102 2 0.4970948482687376 1102 3 0.44138018932275713 1102 4 0.2720373413315684 1102 5 0.16151440091357472 1102 6 0.2360302173505744 1102 7 0.11202383881852995 1102 8 0.25634813750061625 1102 9 0.36444314884847273 1102 10-11 0.3214473390451076 1102 12-13 0.34657132075017927 1102 14-15 0.3832370973838266 1102 16-17 0.39074229348801026 1102 18-19 0.2567351903874808 1102 20-21 0.44310127139359423 1102 22-23 0.5316488209800454 1102 24-25 0.269673896710124 1102 26-27 0.33266432243655686 1102 28-29 0.28449475706779737 1102 30-31 0.48175636777619246 1102 32-33 0.5205043708559671 1102 34-35 0.6160567948983626 1102 36-37 0.7083734222967379 1102 38-39 0.8485972706885363 1102 40-41 0.8209048045360632 1102 42-43 0.8893179636591881 1102 44-45 0.8429378616078083 1102 46-47 0.7627123368625135 1102 48-49 0.6254751334605047 1102 50-51 0.697537073656612 1102 52-53 0.4059129356047251 1102 54-55 0.6450620403036709 1102 56-57 0.5486342919053868 1102 58-59 0.6264720802678809 1102 60-61 0.7231014671657832 1102 62-63 0.888103215437603 1102 64-65 0.8254820423782832 1102 66-67 0.9198979075013085 1102 68-69 1.1363767380778569 1102 70-71 0.993553685334085 1102 72-73 0.9166028704021372 1102 74-75 0.7923636670416272 1102 76-77 0.8623080655454629 1102 78-79 0.9857554183713546 1102 80-81 0.6805359100222041 1102 82-83 0.8351645473870875 1102 84-85 0.6984651917790821 1102 86-87 0.7612308029953425 1102 88-89 1.016127030177124 1102 90-91 0.7986719118879364 1102 92-93 0.5207582981976913 1102 94-95 0.76490980324402 1102 96-97 0.4571196963125317 1102 98-99 0.3297288361899895 1102 100-101 0.926817588810998 1103 1 0.16595811127170634 1103 2 0.17572872302142173 1103 3 0.19492318844850587 1103 4 -0.009677102141495197 1103 5 0.00294218354827791 1103 6 0.019734722020885442 1103 7 0.18937165090153485 1103 8 0.1525773741453449 1103 9 0.29745975953268555 1103 10-11 0.1495256071167077 1103 12-13 0.1983117615544856 1103 14-15 0.25184704538916236 1103 16-17 0.14786280147126263 1103 18-19 -0.029724341200427773 1103 20-21 0.11328026186890128 1103 22-23 0.1321469114415521 1103 24-25 0.21091532679340474 1103 26-27 0.3377602594908069 1103 28-29 0.14001976512006564 1103 30-31 0.1615635734081735 1103 32-33 0.19985719835332105 1103 34-35 0.29435707557750845 1103 36-37 0.3386879209853646 1103 38-39 0.3191547178886509 1103 40-41 0.526352740854108 1103 42-43 0.45307469305688386 1103 44-45 0.41348150491521807 1103 46-47 0.21258695150383744 1103 48-49 0.35640850667174817 1103 50-51 0.5959116652914602 1103 52-53 0.15034536554997402 1103 54-55 0.2498911020990917 1103 56-57 -0.07036101477109469 1103 58-59 0.07182016842875072 1103 60-61 0.44139852693664494 1103 62-63 0.3308918318274223 1103 64-65 0.19950426664552623 1103 66-67 0.3076435017588963 1103 68-69 0.20791541198390462 1103 70-71 0.3863204455604645 1103 72-73 0.3059531671858302 1103 74-75 0.215165397129514 1103 76-77 0.026091252404157217 1103 78-79 0.23680221356760356 1103 80-81 0.26674121991959865 1103 82-83 0.352131832161394 1103 84-85 0.4664102522861455 1103 86-87 0.3109248167072103 1103 88-89 0.5026912412846549 1103 90-91 0.27090883269960386 1103 92-93 0.37094004945154424 1103 94-95 0.6062283050615314 1103 96-97 0.8533661878231378 1103 98-99 0.8517575482868018 1103 100-101 1.432615223744051 1104 1 0.17015144125619486 1104 2 0.20226463932954175 1104 3 0.03628645749867587 1104 4 0.0518905917454191 1104 5 0.2093981645035683 1104 6 0.17564142042873243 1104 7 0.1535208635601535 1104 8 0.12929565662727782 1104 9 0.23175333631212425 1104 10-11 0.25575117332545716 1104 12-13 0.3685893512633598 1104 14-15 0.15724049767911907 1104 16-17 0.26473003452868227 1104 18-19 0.24302775000450083 1104 20-21 0.1509219505578372 1104 22-23 0.30469588822535343 1104 24-25 0.08660602502652637 1104 26-27 0.04725203663279132 1104 28-29 0.06161323051417611 1104 30-31 0.10441307217420359 1104 32-33 0.24334115668210643 1104 34-35 0.339637395318924 1104 36-37 0.293129137924673 1104 38-39 0.3833104826253688 1104 40-41 0.2592408968809252 1104 42-43 0.3344602391725928 1104 44-45 0.31296716678431125 1104 46-47 0.22000171988788253 1104 48-49 -0.19180932808484386 1104 50-51 0.08705543972524765 1104 52-53 0.07787392169036167 1104 54-55 0.059171028715816476 1104 56-57 0.0991521658260126 1104 58-59 8.065619465540408E-4 1104 60-61 0.10850927889681117 1104 62-63 0.16825287317103488 1104 64-65 0.05322912304581706 1104 66-67 0.0765407870145296 1104 68-69 0.16775117322617916 1104 70-71 0.252412349375966 1104 72-73 0.10225451909794714 1104 74-75 0.23597916522962592 1104 76-77 -0.0502349974047398 1104 78-79 0.2974416963902371 1104 80-81 -0.007867293413884369 1104 82-83 0.30809640725912857 1104 84-85 0.1481430585411978 1104 86-87 0.4205301632029368 1104 88-89 0.6350814393758171 1104 90-91 0.2570238871910533 1104 92-93 -0.27714783575604685 1104 94-95 -0.013707484573078688 1104 96-97 -0.030034122308556732 1104 98-99 0.16947873552335224 1104 100-101 0.6355330825249261 1105 1 0.25905832048565713 1105 2 0.2142731916606735 1105 3 0.16927313080793027 1105 4 0.12242656550719744 1105 5 -0.11807100973980056 1105 6 0.04124319732083137 1105 7 0.24637177899171547 1105 8 0.07632102300748045 1105 9 0.31296934494748285 1105 10-11 0.168152053468269 1105 12-13 0.23168458935859348 1105 14-15 0.2716850113171745 1105 16-17 0.2630745772280605 1105 18-19 0.15012493935975613 1105 20-21 0.2944744892716642 1105 22-23 0.25892949971704127 1105 24-25 0.18353071469315552 1105 26-27 0.12271286612588028 1105 28-29 0.16805550227902444 1105 30-31 0.2939447697704125 1105 32-33 0.22185134890197844 1105 34-35 0.21127458550450484 1105 36-37 0.2191317534434134 1105 38-39 0.0865269479386015 1105 40-41 0.28472663607634274 1105 42-43 0.32084293523271157 1105 44-45 0.3683030326040537 1105 46-47 0.30282648013199065 1105 48-49 0.2656833027948835 1105 50-51 0.20691781361988149 1105 52-53 0.19933985767242746 1105 54-55 0.32628088050309145 1105 56-57 0.14257289069835366 1105 58-59 0.49673797723280444 1105 60-61 0.46834549760399824 1105 62-63 0.3181521964574543 1105 64-65 0.18849918573523183 1105 66-67 0.40325748066670997 1105 68-69 0.2834619300659682 1105 70-71 0.30682448040099786 1105 72-73 0.15394215143074064 1105 74-75 0.06027235242604334 1105 76-77 -0.11162703460322732 1105 78-79 0.2170923378150711 1105 80-81 0.21119278048490386 1105 82-83 0.14577855945741192 1105 84-85 0.20687287130326482 1105 86-87 0.13647133478927387 1105 88-89 0.12526286504111184 1105 90-91 0.16513303320342487 1105 92-93 0.5623921650742751 1105 94-95 0.4588658952583664 1105 96-97 0.2595774939159625 1105 98-99 0.3235296758646129 1105 100-101 0.2117930983010723 1106 1 0.1077954504727856 1106 2 0.2180336485461325 1106 3 0.23285903814383602 1106 4 0.09127711248274295 1106 5 0.08807039952660745 1106 6 0.1896103144379495 1106 7 0.14574436586429584 1106 8 0.12900763819409633 1106 9 0.06095840543654418 1106 10-11 0.06835918023789844 1106 12-13 -0.06333029159378611 1106 14-15 0.25063047463763866 1106 16-17 0.3046205875240702 1106 18-19 0.31992867166349015 1106 20-21 0.06059216945184431 1106 22-23 0.21753430988434275 1106 24-25 0.24408010336754415 1106 26-27 0.22539143755444968 1106 28-29 0.020462596873620953 1106 30-31 0.3758704454460897 1106 32-33 0.28294369124431995 1106 34-35 -0.006992789012876699 1106 36-37 0.03621669552835982 1106 38-39 0.11080904253319801 1106 40-41 0.05553986001456224 1106 42-43 0.16244122995600208 1106 44-45 0.2975581806091938 1106 46-47 0.07376036044087186 1106 48-49 0.16572955440363302 1106 50-51 0.3143019835040448 1106 52-53 0.0218543364369026 1106 54-55 0.27777701949922573 1106 56-57 0.3397555644435286 1106 58-59 0.30408997208480315 1106 60-61 0.1225141752101706 1106 62-63 0.09275604137379645 1106 64-65 0.0956038350273829 1106 66-67 0.20128877088549757 1106 68-69 0.3178087769128126 1106 70-71 0.30558775260176674 1106 72-73 0.3402657518168368 1106 74-75 -0.03727832324963387 1106 76-77 -0.2068450204462131 1106 78-79 -0.008685895775659702 1106 80-81 0.04228391626353911 1106 82-83 -0.1095303208514693 1106 84-85 0.13208603089142557 1106 86-87 0.3817556816361183 1106 88-89 0.1788242389149879 1106 90-91 0.3799717558546476 1106 92-93 0.30713355825317024 1106 94-95 0.3511398656573341 1106 96-97 0.5521591380601052 1106 98-99 0.3352333438182846 1106 100-101 0.2703617115571859 1107 1 0.3596729538105734 1107 2 0.49058454974773724 1107 3 0.456782602896844 1107 4 0.24817380807687073 1107 5 0.2267761738924321 1107 6 0.21209863219495872 1107 7 0.2564753404971398 1107 8 0.15245556876819677 1107 9 0.27466000757272724 1107 10-11 0.39555585914310143 1107 12-13 0.3570739974582864 1107 14-15 0.5046983985361706 1107 16-17 0.6358538986055109 1107 18-19 0.48940463962076564 1107 20-21 0.46881354795652186 1107 22-23 0.3773411356520313 1107 24-25 0.4977725265985171 1107 26-27 0.3680399689296223 1107 28-29 0.4519024333222177 1107 30-31 0.4182185762872095 1107 32-33 0.5046142519919883 1107 34-35 0.4288800414277105 1107 36-37 0.6310097529382332 1107 38-39 0.7570561219724468 1107 40-41 0.9148731377181747 1107 42-43 0.6733098881669477 1107 44-45 0.6937113848815599 1107 46-47 0.6263930373434121 1107 48-49 0.671330772694688 1107 50-51 0.6895523172824198 1107 52-53 0.7754007316304907 1107 54-55 0.6123063919958867 1107 56-57 0.5798360884755738 1107 58-59 0.7823109333665101 1107 60-61 0.7200504736080333 1107 62-63 0.7057921728824752 1107 64-65 0.7760423363624938 1107 66-67 0.8096311440497175 1107 68-69 0.6197947582212251 1107 70-71 0.5047595650183254 1107 72-73 0.6693816563562223 1107 74-75 0.25609200385317266 1107 76-77 0.43891182201039314 1107 78-79 0.5712853158398268 1107 80-81 0.4674654082528562 1107 82-83 0.33739891813384304 1107 84-85 0.3108869320930303 1107 86-87 0.3454677143463982 1107 88-89 0.7000713183542402 1107 90-91 0.9186587551870922 1107 92-93 0.8963253973719958 1107 94-95 1.3176160005037971 1107 96-97 1.708628180116179 1107 98-99 2.0946208605072023 1107 100-101 2.781991801677343 1108 1 -0.07689788888827565 1108 2 0.029959130764339648 1108 3 -0.04138372858310646 1108 4 -0.04880162470833227 1108 5 0.09423940193769909 1108 6 -0.062103820583146785 1108 7 0.08369644543030574 1108 8 0.08692656525859377 1108 9 0.05293399313998037 1108 10-11 0.014029576560979251 1108 12-13 0.1362204543374972 1108 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-1.0656767021888562 2316 98-99 -0.6773815050258207 2316 100-101 -0.3208458684665274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 7.0 6 12.0 7 54.0 8 141.0 9 266.0 10 520.0 11 974.0 12 1656.0 13 2309.0 14 3024.0 15 4379.0 16 6329.0 17 9268.0 18 7312.0 19 3579.0 20 2562.0 21 2498.0 22 2520.0 23 2829.0 24 3136.0 25 3658.0 26 4236.0 27 4904.0 28 6074.0 29 7448.0 30 9467.0 31 11608.0 32 14701.0 33 19641.0 34 26402.0 35 39133.0 36 57628.0 37 100021.0 38 96128.0 39 9740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.275308633994236 23.30447936801412 16.646087475568944 19.7741245224227 2 14.325693825053968 23.409728416126992 41.84602060469746 20.418557154121586 3 16.878875229982377 28.76233933549635 33.74977917383005 20.609006260691217 4 10.57878125235638 20.52330529014467 41.6843148449366 27.213598612562347 5 9.646527444063501 41.41208008567987 36.527939696543285 12.413452773713347 6 24.833787912083178 42.686237251328194 18.919955360797648 13.560019475790988 7 23.77296053567043 37.761921381574695 22.81015843469793 15.654959648056947 8 24.531525359461916 36.08773585312151 21.541862178617134 17.83887660879944 9 26.340360991541818 16.78817492017942 23.390985121702148 33.48047896657661 10-11 22.24882908269886 29.473184162562532 29.666326271204696 18.61166048353391 12-13 23.563883305217484 28.73763064175236 29.98862439163117 17.709861661398985 14-15 21.027714222928868 29.59997070013745 29.601801941546775 19.770513135386906 16-17 16.869072702438352 32.94543331480548 30.927728441979806 19.25776554077636 18-19 16.763614741277905 30.118642899307574 36.065653236126735 17.05208912328779 20-21 17.807530064675138 28.95289616214889 36.441488605369635 16.798085167806345 22-23 18.10960601082595 28.71655939460857 36.01637357606442 17.157461018501063 24-25 17.71682975487218 29.42611048633463 36.23391200561868 16.62314775317451 26-27 17.117727532235307 29.620716770975836 36.592267835791155 16.669287860997706 28-29 16.794800947515036 29.428227028738917 37.094093327430215 16.682878696315832 30-31 17.69280817638517 28.968515574169583 36.659621773244915 16.679054476200324 32-33 16.77180146757841 29.623669118375755 36.8542719630477 16.750257450998134 34-35 17.399163230396024 29.490203935660947 36.21150622837519 16.899126605567837 36-37 17.792987853483453 28.941585553444245 35.56927909411719 17.696147498955114 38-39 16.612268024801473 30.34851755621911 36.08224212889354 16.956972290085876 40-41 17.01386906525193 29.798508065602025 36.47246384617456 16.71515902297148 42-43 17.052322662523892 29.571472588940768 35.65042954012165 17.725775208413687 44-45 17.912126265172372 29.587259730355086 34.883425326284126 17.617188678188406 46-47 18.290331476239103 29.775015834852187 34.32015701279283 17.614495676115872 48-49 17.678373685276387 30.266650293214063 34.18464514850291 17.87033087300664 50-51 17.923329153794118 30.046470443763653 33.53358496744699 18.49661543499524 52-53 17.957691860239656 30.758931072073352 33.541879413830394 17.741497653856594 54-55 18.777226250953323 30.09785292330761 32.84223747538596 18.282683350353107 56-57 18.67263005045609 31.11031398249764 32.15347526531456 18.06358070173171 58-59 18.364119733026545 30.41077976413611 32.7046789295209 18.520421573316444 60-61 18.565448567969216 30.91641783327517 32.62431974767648 17.89381385107914 62-63 18.29302447831164 30.786399693213202 33.10205831534408 17.818517513131077 64-65 18.267063938332406 30.361336246084374 32.985182025396085 18.38641779018713 66-67 18.450726679679253 31.23656191965805 31.742415428962918 18.570295971699778 68-69 18.29313219839454 30.114226375908622 32.662452657023564 18.930188768673275 70-71 18.513527488010755 30.32729669988754 33.219904086038184 17.93927172606352 72-73 18.575574255761946 29.967296182831145 33.020514212587734 18.43661534881917 74-75 18.328679825751994 31.218464945730624 31.858106797998992 18.594748430518393 76-77 17.750115260488705 30.779721048073316 32.969347173209584 18.50081651822839 78-79 17.653921226457776 30.593580744819743 32.299220537480124 19.453277491242357 80-81 17.89286364454058 30.811100781940926 32.00894939412774 19.287086179390755 82-83 18.0674065799353 31.089822655651634 31.983707975917447 18.859062788495617 84-85 17.604787968032817 30.38659868774688 32.13824439017717 19.870368954043133 86-87 17.4444056652146 31.05322233855991 31.882128376485998 19.62024361973949 88-89 17.925698995617946 30.343777872571447 32.32539651762516 19.40512661418544 90-91 17.53488026355054 29.383989390754834 33.27788832067327 19.80324202502136 92-93 18.02476769513501 29.61706592438269 32.99440055326584 19.363765827216454 94-95 17.26515944726671 29.153686396677053 33.51764239517759 20.06351176087865 96-97 18.00444237621885 28.937061309962388 32.58381699650556 20.474679317313203 98-99 17.40601540206632 29.246690516278385 32.40767970423896 20.939614377416333 100-101 17.09270029110921 28.75387588965 33.60749169875476 20.54593212048603 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 892.0 1 602.0 2 393.0 3 476.0 4 388.5 5 388.0 6 774.0 7 1489.0 8 2066.0 9 2182.0 10 1874.5 11 1526.0 12 1406.0 13 1382.0 14 1401.5 15 1403.5 16 1443.0 17 1547.5 18 1640.5 19 1752.0 20 1908.5 21 2160.0 22 2430.5 23 2651.5 24 3027.5 25 3799.0 26 5405.0 27 7614.5 28 8093.5 29 8799.0 30 10913.0 31 12460.0 32 15105.0 33 18504.0 34 21554.5 35 24308.0 36 28414.5 37 31497.5 38 28380.0 39 23550.5 40 20941.0 41 17893.0 42 14528.0 43 14275.0 44 14790.0 45 13743.0 46 11662.0 47 10173.5 48 10786.0 49 9633.5 50 7434.0 51 5889.0 52 4631.5 53 3862.0 54 3182.0 55 2827.0 56 2592.0 57 2182.0 58 1732.5 59 1413.0 60 1150.0 61 897.0 62 671.5 63 458.0 64 304.0 65 270.5 66 245.5 67 192.0 68 143.0 69 111.5 70 102.0 71 82.0 72 57.5 73 39.0 74 23.5 75 22.5 76 21.5 77 10.5 78 10.0 79 10.5 80 7.0 81 4.5 82 2.0 83 1.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022621217409288918 2 0.0 3 0.0 4 2.1544016580275158E-4 5 0.02434473873571093 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003231602487041274 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.540405803096306E-4 24-25 0.0 26-27 2.1544016580275158E-4 28-29 9.694807461123822E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.540405803096306E-4 42-43 0.014003610777178856 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0772008290137579E-4 82-83 0.002693002072534395 84-85 5.38600414506879E-4 86-87 0.0 88-89 0.0 90-91 0.009156207046616942 92-93 0.002800722155435771 94-95 0.0 96-97 0.0 98-99 0.001615801243520637 100-101 0.017019773098417377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 464166.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.627417220285835 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1478637855623 27.060586841767314 2 11.01577536787991 7.628956993370506 3 3.5314384666936247 3.6685377952189984 4 1.6176923164142258 2.2406602709810417 5 0.8778318577370415 1.5198524993559568 6 0.6134166751960856 1.2744621085137249 7 0.44454877695828704 1.0775503182151676 8 0.3616964947189272 1.0019692343797764 9 0.2775985285807026 0.8651268062011287 >10 2.3873953955408194 17.56565977460013 >50 0.42609851058427695 10.496599607463983 >100 0.27927571001638096 17.768152627095294 >500 0.014994668994168101 3.452640940177207 >1k 0.0031238893737850215 1.91226851746515 >5k 0.0012495557495140087 2.466975665194604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6324 1.3624436085366012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5079 1.0942206021121752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3977 0.8568055393975432 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1400 0.30161623212385225 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1332 0.2869663008492651 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.23504522089080201 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1039 0.22384233226905892 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 998 0.21500928547114612 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 951 0.20488359767841677 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 900 0.19389614922247644 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 845 0.1820469401033251 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 811 0.17472197446603155 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 768 0.16545804733651323 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 738 0.15899484236243067 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 684 0.14736107340908208 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 672 0.14477579141944907 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 652 0.14046698810339403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 632 0.13615818478733901 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 610 0.13141850113967848 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 0.12840233881843996 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.12732513798942618 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 587 0.12646337732621518 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 586 0.12624793716041244 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 584 0.12581705682880695 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 580 0.12495529616559592 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 0.12258545434176565 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 559 0.12043105268373816 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 516 0.11116712555421984 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 512 0.11030536489100881 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 510 0.10987448455940332 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 508 0.10944360422779781 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 487 0.10491936074594004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.154401658027516E-4 0.0 22-23 0.0 0.0 0.0 9.694807461123822E-4 0.0 24-25 0.0 0.0 0.0 0.001723521326422013 0.0 26-27 0.0 0.0 0.0 0.0023698418238302676 0.0 28-29 0.0 0.0 0.0 0.0042010832331536565 0.0 30-31 0.0 0.0 0.0 0.00818672630050456 0.0 32-33 0.0 0.0 0.0 0.012926409948165095 0.0 34-35 0.0 0.0 0.0 0.01723521326422013 0.0 36-37 0.0 0.0 0.0 0.02057453583416278 0.0 38-39 0.0 0.0 0.0 0.026068260062132944 0.0 40-41 0.0 0.0 0.0 0.034254986362637505 0.0 42-43 0.0 0.0 0.0 0.045134714735676464 0.0 44-45 0.0 0.0 0.0 0.06710961164755712 0.0 46-47 0.0 0.0 0.0 0.12355493508787804 0.0 48-49 0.0 0.0 0.0 0.1651348870878091 0.0 50-51 0.0 0.0 0.0 0.2163019264659626 0.0 52-53 0.0 0.0 0.0 0.2741476109840014 0.0 54-55 0.0 0.0 0.0 0.33640981890099664 0.0 56-57 0.0 0.0 0.0 0.41073667610294595 0.0 58-59 0.0 0.0 0.0 0.5189953594188286 0.0 60-61 0.0 0.0 0.0 0.6436274953357204 0.0 62-63 0.0 0.0 0.0 0.7816169215323828 0.0 64-65 0.0 0.0 0.0 0.9109887410969352 0.0 66-67 0.0 0.0 0.0 1.1073624522261434 0.0 68-69 0.0 0.0 0.0 1.3350827074796516 0.0 70-71 0.0 0.0 0.0 1.51659104716847 0.0 72-73 0.0 0.0 0.0 1.757129992287242 0.0 74-75 0.0 0.0 0.0 1.9772021216547528 0.0 76-77 0.0 0.0 0.0 2.204599216659557 0.0 78-79 0.0 0.0 0.0 2.480254908804178 0.0 80-81 0.0 0.0 0.0 2.7647436477467116 0.0 82-83 0.0 0.0 0.0 3.0784245291555177 0.0 84-85 0.0 0.0 0.0 3.4791432375486355 0.0 86-87 0.0 0.0 0.0 3.9730398176514434 0.0 88-89 0.0 0.0 0.0 4.540616934458793 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCCCG 45 1.5152684E-5 52.77359 6 CTCCCGC 50 2.8238968E-5 47.501343 7 ATGGGCG 50 2.8256958E-5 47.496227 5 TGGGCGA 45 9.622893E-4 42.218872 6 TACTCCC 60 8.286985E-5 39.580193 5 GGTATCA 2745 0.0 38.251583 1 CGTGCAA 75 6.912027E-6 38.001076 9 CTGACCG 75 6.91714E-6 37.996986 4 TGACCGT 75 6.91714E-6 37.996986 5 TACGTTG 25 0.0016162863 37.996983 32-33 CATGGGT 490 0.0 35.8645 4 GGCAATT 55 0.0025776895 34.546432 8 GTATCAA 4335 0.0 34.195187 1 ATGGGTA 320 0.0 34.137913 5 TCCCGCT 70 2.0511105E-4 33.929535 8 CCGTGCA 85 1.6297994E-5 33.53036 8 GTGGTAC 75 3.0712446E-4 31.674389 1 AGTCTGC 120 1.5021578E-7 31.667564 8 GGGGCAT 75 3.0770738E-4 31.664154 6 ACCGTGC 95 3.4857327E-5 30.00085 7 >>END_MODULE