FastQCFastQC Report
Thu 26 May 2016
SRR937653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455007
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54261.1925091262332228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.9193265158558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.6597700694714587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17160.37713705503431816No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10430.22922724265780525No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA8850.19450250215930742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.19054651906454187No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8670.19054651906454187No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.18681031280837437No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT8480.1863707591311782No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC7290.1602173153380058No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT7100.15604155540464212No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7010.15406356385725933No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA6760.14856914289230716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6600.14505271347473775No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6550.14395382928174733No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5850.1285694505798812No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA5820.12791012006408692No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5780.12703101270969458No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA5510.12109703806754621No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT5460.11999815387455579No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA5320.11692127813418254No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5310.11670150129558447No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG5180.11384440239380932No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5150.11318507187801506No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC5000.10988841929904375No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.10681154355867054No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4810.10571265936568008No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC4630.1017566762709145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.039.4877621
GACGTGG651.3261662E-436.541987
GTATCAA38300.035.2564051
CTGTAGG1502.5465852E-1134.8674241
GCAGGGC550.002576770334.5487828
AGTGGAC851.6289612E-533.532648
TGTAGGA1657.4578566E-1131.6941662
TGGACTG902.399125E-531.6871765
GGACGTG902.408183E-531.6697186
CTTAACG300.00395078731.66622520-21
GTTCGCA650.005837645429.2335839
TGTTCGC650.005837645429.2335838
CATTCCG3100.029.1157059
TAGGACG1004.9248018E-528.518464
GTACATG33650.028.4007281
GGCATTC3200.028.205847
TAGGGTC1354.1918065E-728.166384
GTCCAGG856.364635E-427.9685251
TCTAGAC856.3810777E-427.956193
TCTACAC1754.9167284E-927.1574423
Produced by FastQC (version 0.11.5)