##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430052 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93589147358924 33.0 31.0 34.0 30.0 34.0 2 32.02449238696716 33.0 31.0 34.0 30.0 34.0 3 32.03073116739371 33.0 31.0 34.0 30.0 34.0 4 35.68047119883177 37.0 35.0 37.0 35.0 37.0 5 35.72008268767498 37.0 35.0 37.0 35.0 37.0 6 35.81605247737483 37.0 35.0 37.0 35.0 37.0 7 35.75644108154363 37.0 35.0 37.0 35.0 37.0 8 35.768874461693 37.0 35.0 37.0 35.0 37.0 9 37.34901825825714 39.0 37.0 39.0 35.0 39.0 10-11 37.29508989610559 39.0 37.0 39.0 34.0 39.0 12-13 37.2336717420219 39.0 37.0 39.0 34.0 39.0 14-15 38.348638071675055 40.0 38.0 41.0 34.0 41.0 16-17 38.291121771320675 40.0 38.0 41.0 34.0 41.0 18-19 38.36563369080949 40.0 38.0 41.0 34.0 41.0 20-21 38.42445797252425 40.0 38.0 41.0 34.0 41.0 22-23 38.40608228772335 40.0 38.0 41.0 34.0 41.0 24-25 38.340558816143165 40.0 38.0 41.0 34.0 41.0 26-27 38.2391338721829 40.0 38.0 41.0 34.0 41.0 28-29 38.10484894849925 40.0 38.0 41.0 34.0 41.0 30-31 37.821034433045305 40.0 37.0 41.0 33.5 41.0 32-33 37.55896379972654 40.0 36.5 41.0 33.0 41.0 34-35 37.44546124654693 40.0 36.0 41.0 33.0 41.0 36-37 37.26656078799773 40.0 36.0 41.0 33.0 41.0 38-39 37.06894863876927 40.0 36.0 41.0 33.0 41.0 40-41 36.829560146214874 39.5 35.5 41.0 31.5 41.0 42-43 36.49563541153162 39.0 35.0 41.0 31.0 41.0 44-45 36.107768362895655 39.0 35.0 41.0 30.0 41.0 46-47 35.539717290002145 39.0 35.0 41.0 28.0 41.0 48-49 35.0666919349288 39.0 35.0 40.5 24.5 41.0 50-51 34.16172695394975 38.0 34.0 39.5 22.5 40.5 52-53 34.2737215499521 38.0 34.5 40.0 23.0 40.5 54-55 34.59105991833546 39.0 35.0 40.0 22.5 41.0 56-57 34.41212341763322 39.0 35.0 40.0 21.0 41.0 58-59 34.195914680085195 38.5 34.5 40.0 19.0 41.0 60-61 33.91868541478705 38.0 34.0 40.0 16.5 41.0 62-63 33.574238696715746 38.0 34.0 40.0 11.5 41.0 64-65 33.19092226056384 38.0 33.5 40.0 8.0 41.0 66-67 32.734697199408444 37.0 33.0 39.5 6.0 41.0 68-69 32.26594923404612 36.5 32.0 39.0 2.0 40.5 70-71 31.84975072781896 36.0 32.0 39.0 2.0 40.0 72-73 31.374008259466297 35.5 31.0 38.5 2.0 40.0 74-75 30.831486657427476 35.0 31.0 37.5 2.0 39.5 76-77 30.31535721261615 35.0 30.0 37.0 2.0 39.0 78-79 29.8208495716797 34.5 30.0 36.5 2.0 39.0 80-81 29.28268093160827 34.0 29.0 36.0 2.0 38.0 82-83 28.741022015942256 34.0 29.0 35.5 2.0 37.0 84-85 28.21064313152828 34.0 27.5 35.0 2.0 37.0 86-87 27.68991075497847 34.0 26.5 35.0 2.0 36.0 88-89 27.1655485848223 33.0 26.0 35.0 2.0 36.0 90-91 26.65647875140681 33.0 25.5 35.0 2.0 35.5 92-93 26.06019504618046 32.5 24.5 35.0 2.0 35.0 94-95 25.38024703989285 32.0 20.5 34.5 2.0 35.0 96-97 24.555851385413856 32.0 12.5 34.0 2.0 35.0 98-99 23.515288848790377 31.0 2.0 34.0 2.0 35.0 100-101 20.326820942583687 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08951422698708811 1101 2 0.023781032597753438 1101 3 0.02709588058677781 1101 4 0.010420920209135431 1101 5 0.016954228410355654 1101 6 -0.04523734417615799 1101 7 -0.09729413243486817 1101 8 0.01652738884003213 1101 9 0.038327026980716994 1101 10-11 -0.0019942821909566533 1101 12-13 0.35336494830840337 1101 14-15 0.4216529346900657 1101 16-17 0.48294016816025476 1101 18-19 0.3989943173696915 1101 20-21 0.17451336001821005 1101 22-23 0.14890572792507584 1101 24-25 0.3225701841085211 1101 26-27 0.05613476111823701 1101 28-29 0.06124459442037278 1101 30-31 0.04697333731376574 1101 32-33 0.2120431867339576 1101 34-35 0.126620349902403 1101 36-37 0.3596017725525229 1101 38-39 -0.20552750448516832 1101 40-41 -0.14429737483309424 1101 42-43 -0.2154689316368632 1101 44-45 0.17684449719818218 1101 46-47 0.02091016134376389 1101 48-49 0.13332674204971795 1101 50-51 0.13561034401895 1101 52-53 0.3616072674199984 1101 54-55 0.5006074428709724 1101 56-57 0.47209733458760894 1101 58-59 0.2687852725276869 1101 60-61 0.5088466061255943 1101 62-63 0.6385688073941012 1101 64-65 0.8430460575092056 1101 66-67 1.0181254976462313 1101 68-69 0.9206002251915919 1101 70-71 0.6145029950674079 1101 72-73 0.937871198618371 1101 74-75 0.8111635233788412 1101 76-77 0.48171922185197147 1101 78-79 0.6958202539223706 1101 80-81 1.1196593520670142 1101 82-83 1.2246510347006065 1101 84-85 1.1959393383404269 1101 86-87 1.4142618830224514 1101 88-89 1.373007178010269 1101 90-91 1.545309089112866 1101 92-93 1.5171066330672183 1101 94-95 1.5638628668993526 1101 96-97 1.84717222183831 1101 98-99 2.26064273900478 1101 100-101 3.0570753525976784 1102 1 -0.029850307147736288 1102 2 0.23749936664670201 1102 3 0.17755387400312372 1102 4 0.1489818190212091 1102 5 0.16734066035539996 1102 6 0.12443490428526616 1102 7 0.28953140069809535 1102 8 0.09835793127598436 1102 9 0.42966093280581674 1102 10-11 0.38752375129980976 1102 12-13 0.2832436516145407 1102 14-15 0.7250109567309977 1102 16-17 0.6220467231910263 1102 18-19 0.5345227988352761 1102 20-21 0.5216938380488401 1102 22-23 0.4950217290700607 1102 24-25 0.4393316697246661 1102 26-27 0.6925488157912056 1102 28-29 0.46149684860672124 1102 30-31 0.501639145242784 1102 32-33 0.4595116996865798 1102 34-35 0.6212711299224409 1102 36-37 0.8855428059001724 1102 38-39 0.7479128132477726 1102 40-41 0.6718254246516651 1102 42-43 0.5379034026983263 1102 44-45 0.8668670390616455 1102 46-47 0.7359434374279132 1102 48-49 0.7426279442824395 1102 50-51 0.452475593768483 1102 52-53 0.6064816770378627 1102 54-55 0.7313126811706638 1102 56-57 0.7613219660460331 1102 58-59 0.5149298982611867 1102 60-61 0.6129148757692207 1102 62-63 0.6674438610652089 1102 64-65 0.6420030490649467 1102 66-67 1.0409178262565035 1102 68-69 0.8585832651228955 1102 70-71 1.114860802423923 1102 72-73 0.9164295927789574 1102 74-75 1.2069431856086972 1102 76-77 0.9993143986088064 1102 78-79 1.125305369136342 1102 80-81 1.3370273211524584 1102 82-83 1.570865432868633 1102 84-85 1.7507285898074336 1102 86-87 1.7339359205206613 1102 88-89 1.656685795442641 1102 90-91 1.6516315451025605 1102 92-93 2.300776305315683 1102 94-95 2.346137090457386 1102 96-97 2.344229256330941 1102 98-99 2.6016689305032763 1102 100-101 2.979797908773431 1103 1 0.10855372684499898 1103 2 0.13169521276257257 1103 3 0.04080716220087055 1103 4 0.28553458886499783 1103 5 0.2551611053640386 1103 6 0.3013099704018458 1103 7 0.24562999373733874 1103 8 0.268374504497892 1103 9 0.45300449216968985 1103 10-11 0.2593193012134165 1103 12-13 0.32162652830272265 1103 14-15 0.5759048528486304 1103 16-17 0.55865414940493 1103 18-19 0.4205025583472448 1103 20-21 0.43630648306453423 1103 22-23 0.5317737301984522 1103 24-25 0.4420821193173836 1103 26-27 0.505729816531705 1103 28-29 0.60594834548602 1103 30-31 0.7347397482259552 1103 32-33 0.8748912969925513 1103 34-35 0.8345984686861527 1103 36-37 0.7891272059142764 1103 38-39 0.8831873292744277 1103 40-41 0.8565551400859235 1103 42-43 0.8588597128644153 1103 44-45 1.0287733122024534 1103 46-47 0.9514834776267946 1103 48-49 0.8539682595262761 1103 50-51 0.995424922024057 1103 52-53 1.0748603222651312 1103 54-55 1.1796888580457363 1103 56-57 1.2214757091258335 1103 58-59 1.0171690463270622 1103 60-61 1.4026729772537578 1103 62-63 1.5811661361806841 1103 64-65 1.6238253982309985 1103 66-67 1.6926068844038298 1103 68-69 1.9121994010938366 1103 70-71 1.953377675374309 1103 72-73 1.7056852082802116 1103 74-75 1.7274216933134774 1103 76-77 2.029939596958531 1103 78-79 2.1488076082844074 1103 80-81 2.0029358510724116 1103 82-83 2.242980105459342 1103 84-85 2.669458093668645 1103 86-87 2.675788595156579 1103 88-89 2.480939118902569 1103 90-91 2.4605193761262214 1103 92-93 2.7143927939083703 1103 94-95 2.98060057647594 1103 96-97 2.8111357057826147 1103 98-99 3.3218106844438253 1103 100-101 3.994220138048089 1104 1 0.11595901542877485 1104 2 0.05977174467205515 1104 3 -0.059720424882780065 1104 4 0.12484300483245647 1104 5 -0.1454554723120367 1104 6 0.07924665995180646 1104 7 0.042540748886082724 1104 8 0.18125907098657734 1104 9 0.3199317741427379 1104 10-11 0.132809639377669 1104 12-13 0.18753677493378262 1104 14-15 0.3501230069294934 1104 16-17 0.4297925933408564 1104 18-19 0.3457822430662887 1104 20-21 0.24810727126692456 1104 22-23 0.32008735847202985 1104 24-25 0.37371827492379595 1104 26-27 0.3073602513143143 1104 28-29 0.389224470581361 1104 30-31 0.3844346376233645 1104 32-33 0.6382557770332298 1104 34-35 0.3707508968580342 1104 36-37 0.08128649907471441 1104 38-39 0.45198023545291477 1104 40-41 0.35421913703481067 1104 42-43 0.49983034596891684 1104 44-45 0.5246257149484421 1104 46-47 0.7929689848731698 1104 48-49 1.1887317988092718 1104 50-51 0.9650092088280289 1104 52-53 1.069489104365303 1104 54-55 1.0929707039648804 1104 56-57 1.238415704040655 1104 58-59 1.4620902260880584 1104 60-61 1.6839627051968051 1104 62-63 1.3444511603352822 1104 64-65 1.521645128716635 1104 66-67 1.5441832891838985 1104 68-69 1.5163819589377212 1104 70-71 1.4716366379982624 1104 72-73 1.2174764617762754 1104 74-75 1.3027140909743018 1104 76-77 1.3385303673628037 1104 78-79 1.557210862797504 1104 80-81 1.529553572913244 1104 82-83 1.489356917053545 1104 84-85 1.7481194208997657 1104 86-87 1.9310962483475294 1104 88-89 1.7950918013332853 1104 90-91 1.6410908228589953 1104 92-93 1.8020008539134516 1104 94-95 2.1466157048766483 1104 96-97 2.1007841929425446 1104 98-99 2.1040379918427554 1104 100-101 2.9042760749918983 1105 1 0.13017424099268382 1105 2 0.28459160682744766 1105 3 0.06915645481647203 1105 4 0.2774902479402215 1105 5 0.16602635726189874 1105 6 0.13959283789667154 1105 7 0.15747652582843585 1105 8 0.22284741685123777 1105 9 0.3207149821627837 1105 10-11 0.3180774965205302 1105 12-13 0.24228301553529263 1105 14-15 0.5523864781074366 1105 16-17 0.3589905883283251 1105 18-19 0.33507187339210276 1105 20-21 0.34724182640476187 1105 22-23 0.37818181335925516 1105 24-25 0.37544133256790246 1105 26-27 0.46807453702860613 1105 28-29 0.6480355608069317 1105 30-31 0.6059062854930417 1105 32-33 0.6193217231485235 1105 34-35 0.7479438793141711 1105 36-37 0.5816781030847409 1105 38-39 0.18476923921231503 1105 40-41 0.3096154402929656 1105 42-43 0.39944265799898915 1105 44-45 0.533965470587539 1105 46-47 0.5351564848731698 1105 48-49 0.4632462098368393 1105 50-51 0.4073846787528481 1105 52-53 0.46818114697182267 1105 54-55 0.4729244946916964 1105 56-57 0.315737915820101 1105 58-59 0.2792894742083618 1105 60-61 0.6006489520639704 1105 62-63 0.5873827079543261 1105 64-65 0.5189039006464569 1105 66-67 0.7325643982064491 1105 68-69 0.45627074339887486 1105 70-71 0.3666476029105432 1105 72-73 0.2406985795707577 1105 74-75 0.42955462982142123 1105 76-77 0.46554712801443543 1105 78-79 0.12493877633133366 1105 80-81 0.5995919501062268 1105 82-83 0.6530473932440266 1105 84-85 0.7158316702732002 1105 86-87 0.6642181155154496 1105 88-89 0.6145819329122304 1105 90-91 1.094372464463003 1105 92-93 1.02084875491596 1105 94-95 1.2343154229217639 1105 96-97 1.40306332828089 1105 98-99 1.549683355251279 1105 100-101 2.6103185248665888 1106 1 0.12106917454750388 1106 2 0.10009202210983403 1106 3 0.13596500963374325 1106 4 0.2545043675415499 1106 5 0.22609695526854523 1106 6 0.030093253179060753 1106 7 0.14509072848624527 1106 8 0.09940472582133708 1106 9 0.14874654362458273 1106 10-11 -0.302364779226977 1106 12-13 -0.521857349913212 1106 14-15 -0.28651509996565494 1106 16-17 -0.2776788468876248 1106 18-19 -0.14251845052816492 1106 20-21 -0.11912549087097801 1106 22-23 -0.26576025474705745 1106 24-25 -0.21288923221614908 1106 26-27 -0.20048858589498764 1106 28-29 0.1039429528335063 1106 30-31 0.05324328715416726 1106 32-33 0.3671674457398879 1106 34-35 0.18951364675603344 1106 36-37 -0.10895727897506191 1106 38-39 -0.3237388587943357 1106 40-41 -0.24593896170039642 1106 42-43 0.06630831414516791 1106 44-45 0.37543660846129256 1106 46-47 0.12232944998945072 1106 48-49 0.18162245568401403 1106 50-51 0.26785498606181335 1106 52-53 0.14157192770272076 1106 54-55 0.03590726047242754 1106 56-57 -0.30296536491079706 1106 58-59 -0.5900211570208782 1106 60-61 -0.1330252588994867 1106 62-63 -0.38733178540115887 1106 64-65 -0.13240423888842656 1106 66-67 -0.12637974631181947 1106 68-69 0.0401681686480444 1106 70-71 0.21468539360821737 1106 72-73 0.3147122838896941 1106 74-75 0.3554578690240753 1106 76-77 0.6000103755227393 1106 78-79 0.6647384025771821 1106 80-81 0.35333468266436796 1106 82-83 0.11803908759618409 1106 84-85 -0.05452235796600391 1106 86-87 0.01231819857192562 1106 88-89 0.0013188515168813808 1106 90-91 0.3924933116390825 1106 92-93 0.32209979312193937 1106 94-95 0.3714780059782399 1106 96-97 0.6327456372509879 1106 98-99 0.4362697339888193 1106 100-101 1.462701207590836 1107 1 0.09639645007499453 1107 2 0.28994776419105506 1107 3 0.19613236935447276 1107 4 0.22063712589670814 1107 5 0.26669452187874043 1107 6 0.40515870828456 1107 7 0.3304749884585192 1107 8 0.35093201161629395 1107 9 0.25372113164244325 1107 10-11 0.2516010301061442 1107 12-13 0.535930524207636 1107 14-15 0.8139315348718554 1107 16-17 0.48976558044437724 1107 18-19 0.5789790596109583 1107 20-21 0.6300710797948668 1107 22-23 0.7102857435074696 1107 24-25 0.6897546406726036 1107 26-27 0.5870236067227026 1107 28-29 0.5459137932257221 1107 30-31 0.4509793105639943 1107 32-33 0.6582758188596571 1107 34-35 0.9213710316320558 1107 36-37 0.7523754383102172 1107 38-39 0.6895267092533075 1107 40-41 0.7682789139605788 1107 42-43 0.664311291710753 1107 44-45 0.670197633154558 1107 46-47 0.9319038634603629 1107 48-49 0.9916974081969769 1107 50-51 0.8364365354226635 1107 52-53 1.023087919283391 1107 54-55 1.1513752003050683 1107 56-57 1.207350873089105 1107 58-59 1.0568241635807993 1107 60-61 1.0509677592225941 1107 62-63 1.2059301890328484 1107 64-65 1.3807278520813355 1107 66-67 1.478422861624935 1107 68-69 1.654961022490646 1107 70-71 1.4383570399966352 1107 72-73 1.681386651157002 1107 74-75 1.699643915535706 1107 76-77 1.6030752145801905 1107 78-79 1.651175077971196 1107 80-81 1.6374817127993815 1107 82-83 2.021365747075624 1107 84-85 1.8731484670250147 1107 86-87 1.7228500533899371 1107 88-89 1.7126952055391627 1107 90-91 2.1589469283545206 1107 92-93 2.2595426803338086 1107 94-95 2.4738420903768237 1107 96-97 2.713301620617692 1107 98-99 2.5662199367713043 1107 100-101 3.303398882009443 1108 1 0.25726530032838824 1108 2 0.21816646171296838 1108 3 0.3253187814852865 1108 4 0.2137305736416124 1108 5 0.16466590901939782 1108 6 0.15697130452034003 1108 7 0.31465868480940884 1108 8 0.1505622668996054 1108 9 0.4275958270515332 1108 10-11 0.33639316082236803 1108 12-13 0.3471987685182043 1108 14-15 0.08516320335735372 1108 16-17 0.27153895982139176 1108 18-19 0.3757266017055443 1108 20-21 0.32552252214807 1108 22-23 0.33880691413319397 1108 24-25 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-3.0108352089611294 2316 92-93 -3.128647899344415 2316 94-95 -3.0018190599733927 2316 96-97 -2.891564680905045 2316 98-99 -3.1081999200100476 2316 100-101 -3.3030312732011993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1352.0 3 164.0 4 174.0 5 160.0 6 206.0 7 247.0 8 292.0 9 443.0 10 577.0 11 921.0 12 1596.0 13 2333.0 14 2760.0 15 3970.0 16 5589.0 17 7863.0 18 6006.0 19 3172.0 20 2858.0 21 2757.0 22 3053.0 23 3247.0 24 3780.0 25 4289.0 26 4831.0 27 5558.0 28 6600.0 29 8010.0 30 9908.0 31 12213.0 32 15161.0 33 20323.0 34 28162.0 35 40075.0 36 57763.0 37 88675.0 38 68951.0 39 6013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.05669814489941 23.139300511365757 15.688804075995671 20.115197267739166 2 14.0850785004582 23.071183231472002 42.312364900629824 20.531373367439972 3 16.69977986717409 29.357650548466534 33.030417506156255 20.91215207820312 4 10.194216639345601 20.226372329783537 41.97401894830606 27.605392082564805 5 9.604290544479422 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16.88894325423176 26-27 17.10125442286878 30.830235237783445 35.05813079691445 17.010379542433327 28-29 16.93264342879913 30.48376817436673 35.51637396989105 17.067214426943096 30-31 17.74191481205732 30.278806012581168 35.08442011635074 16.894859059010773 32-33 16.844813243199788 30.70144777590582 35.35003646820043 17.103702512693964 34-35 17.44240780355037 30.790095493757253 34.61648700418047 17.151009698511903 36-37 17.842713750489292 30.118338645641114 34.041990531044384 17.996957072825214 38-39 16.697128226275222 31.543847755105368 34.48493172067624 17.274092297943174 40-41 16.949073572560927 30.999647064167927 34.895336471317265 17.155942891953877 42-43 17.11424680609158 30.733862127532674 34.052940772176214 18.098950294199533 44-45 17.787291546531065 30.835444807520922 33.50120281219238 17.876060833755627 46-47 18.079485706762913 30.926331315103635 33.1220735766471 17.87210940148635 48-49 17.620486840940124 31.236280509118362 33.03711661159451 18.106116038346997 50-51 17.74411527581659 30.98037570893975 32.56660654950811 18.708902465735548 52-53 17.77531540852803 31.789614239174508 32.65171534700764 17.78335500528982 54-55 18.38961141849202 30.990636435763992 32.14905275029214 18.470699395451852 56-57 18.495780133665985 32.0936966401804 31.3684107538454 18.04211247230822 58-59 18.248345188352737 31.283160282657203 31.894427442157287 18.574067086832777 60-61 18.504481314751942 31.727941390576138 31.839639398298324 17.9279378963736 62-63 18.12559688802963 31.65300132771191 32.33688942722042 17.884512357038037 64-65 18.184190020720767 31.071131978116824 32.34174937307745 18.402928628084965 66-67 18.344004388779727 31.9990728586354 31.107689333098833 18.54923341948604 68-69 18.10190906242684 31.00715243265404 31.977581823200623 18.913356681718493 70-71 18.348756355080862 31.159434638029737 32.404884895144804 18.086924111744597 72-73 18.347387389906007 30.921487155652315 32.22287066190937 18.508254792532306 74-75 17.977724424501037 32.11494485857305 31.474006586450365 18.433324130475555 76-77 17.670940046481014 31.693062213498134 32.09438325285555 18.541614487165297 78-79 17.45593148470008 31.47153660843674 31.753444636525032 19.31908727033815 80-81 17.81767054799946 31.783945353816335 31.246010334689238 19.15237376349497 82-83 18.11238959801623 31.843408842874233 31.280843545116454 18.763358013993084 84-85 17.77000562529917 31.12362730471302 31.521125153006974 19.585241916980834 86-87 17.549248102348 31.911885119403284 31.442844480473724 19.096022297774997 88-89 18.25506748689227 31.103262458603343 31.53927426109221 19.10239579341218 90-91 17.77573803488892 30.30089831519308 32.52187546593112 19.401488183986878 92-93 18.34216009774472 30.846542420299965 32.122395211533686 18.68890227042162 94-95 17.85485078929499 30.31398251239589 32.49626405420295 19.334902644106176 96-97 18.604697215731317 30.05640293267293 31.70131373869204 19.637586112903715 98-99 17.936919894136505 30.441812353226226 31.483673165094867 20.137594587542402 100-101 18.026828222973794 29.627158043808404 32.926181625066384 19.419832108151418 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1251.0 1 757.0 2 326.5 3 367.5 4 326.0 5 382.0 6 684.5 7 1244.5 8 1818.5 9 1970.0 10 1692.5 11 1456.0 12 1378.5 13 1305.5 14 1257.0 15 1233.0 16 1235.0 17 1313.0 18 1401.0 19 1542.5 20 1735.0 21 1908.0 22 2108.0 23 2363.5 24 2665.5 25 3352.5 26 5045.0 27 7095.5 28 7583.5 29 8214.5 30 10126.0 31 12024.5 32 14779.5 33 17691.5 34 20591.5 35 23299.5 36 26640.0 37 29322.0 38 26435.5 39 21884.5 40 19093.5 41 16364.0 42 13565.0 43 12967.0 44 13251.5 45 12387.5 46 10710.0 47 9212.5 48 9597.5 49 8697.0 50 6676.0 51 5251.0 52 4191.0 53 3548.0 54 3004.0 55 2618.5 56 2312.0 57 1997.0 58 1678.0 59 1346.5 60 1076.0 61 851.5 62 624.0 63 438.5 64 301.0 65 241.5 66 199.5 67 155.5 68 119.5 69 83.0 70 68.0 71 54.0 72 38.5 73 36.5 74 25.5 75 20.0 76 15.5 77 11.0 78 13.5 79 10.0 80 5.5 81 4.0 82 3.0 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 1.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.324611907397245 2 0.2792685535702659 3 0.2836866239431511 4 0.2792685535702659 5 0.27950108358989145 6 0.20834689758447816 7 0.2023011170742143 8 0.20927701766298032 9 0.20974207770223136 10-11 0.20927701766298032 12-13 0.213927618055491 14-15 0.20939328267279306 16-17 0.2005571419270228 18-19 0.19613907155413765 20-21 0.1931161812990057 22-23 0.20125473198589938 24-25 0.19927822681908236 26-27 0.20706798247653774 28-29 0.2117185828690484 30-31 0.2135788230260527 32-33 0.21183484787886114 34-35 0.21253243793773777 36-37 0.19997581687795896 38-39 0.19125594114200142 40-41 0.18497763061211203 42-43 0.1945113614167589 44-45 0.19753425167189081 46-47 0.2047426822802824 48-49 0.2134625580162399 50-51 0.20695171746672497 52-53 0.21532279817324415 54-55 0.20660292243728667 56-57 0.1797457051705375 58-59 0.1623059536986225 60-61 0.17893185010184814 62-63 0.17253727456214596 64-65 0.17962944016072477 66-67 0.18032703021960134 68-69 0.17614148986634176 70-71 0.17893185010184814 72-73 0.18986076102424823 74-75 0.19474389143638443 76-77 0.19637160157376315 78-79 0.19393003636769507 80-81 0.19020955605368653 82-83 0.1786993200822226 84-85 0.1724210095523332 86-87 0.1793969101410992 88-89 0.19079088110275036 90-91 0.18695413577892905 92-93 0.17916438012147368 94-95 0.18055956023922687 96-97 0.19044208607331206 98-99 0.1906746160929376 100-101 0.16928185428738848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.279927641835094 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36272896643398 28.79274064360795 2 10.376287567702201 7.529019242942179 3 3.302292686105098 3.594208191121627 4 1.5355978733519375 2.228455189286566 5 0.8146955253576919 1.4778547355051947 6 0.5830877727678289 1.269262932291337 7 0.41784055421879235 1.0611457551017442 8 0.3183883435442783 0.9240884852632123 9 0.27136398115982346 0.886055904097083 >10 2.3341597979719637 18.061460933730377 >50 0.40333706364170124 10.237311645498123 >100 0.2667539244434781 18.00282608082882 >500 0.010259766324749158 2.5308877034740163 >1k 0.0025649415811872895 2.20751846844976 >5k 6.412353952968224E-4 1.1971640888020303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5146 1.1965994809929963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4014 0.933375498776892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2853 0.6634081459916475 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1567 0.36437454075321124 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1055 0.245319170704938 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 881 0.20485894729009516 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 874 0.2032312371527164 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 849 0.19741798666207808 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 830 0.19299991628919294 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 816 0.18974449601443547 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 751 0.17463004473877577 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 695 0.1616083636397459 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 658 0.15300475291360113 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 657 0.1527722228939756 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 602 0.13998307181457126 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 593 0.13789030163794147 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 581 0.13509994140243506 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 558 0.1297517509510478 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 518 0.12045055016602643 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 514 0.11952043008752429 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 502 0.1167300698520179 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 498 0.11579994977351576 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 496 0.1153348897342647 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 491 0.11417223963613704 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 488 0.11347464957726042 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 486 0.11300958953800937 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 455 0.10580115892961782 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 451 0.10487103885111568 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 444 0.10324332871373694 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 434 0.10091802851748159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.1626500981276682E-4 0.0 24-25 0.0 0.0 0.0 3.487950294383005E-4 0.0 26-27 0.0 0.0 0.0 5.813250490638341E-4 0.0 28-29 0.0 0.0 0.0 0.0030228902551319376 0.0 30-31 0.0 0.0 0.0 0.005231925441574507 0.0 32-33 0.0 0.0 0.0 0.007557225637829844 0.0 34-35 0.0 0.0 0.0 0.009998790843897947 0.0 36-37 0.0 0.0 0.0 0.013137946108842651 0.0 38-39 0.0 0.0 0.0 0.016974691432663957 0.0 40-41 0.0 0.0 0.0 0.022439146893863997 0.0 42-43 0.0 0.0 0.0 0.033135527796638545 0.0 44-45 0.0 0.0 0.0 0.051737929366681236 0.0 46-47 0.0 0.0 0.0 0.09231441779133687 0.0 48-49 0.0 0.0 0.0 0.1294029559216095 0.0 50-51 0.0 0.0 0.0 0.1748625747584013 0.0 52-53 0.0 0.0 0.0 0.2229962888208868 0.0 54-55 0.0 0.0 0.0 0.2705486778343084 0.0 56-57 0.0 0.0 0.0 0.3286811827406918 0.0 58-59 0.0 0.0 0.0 0.4239022257773478 0.0 60-61 0.0 0.0 0.0 0.5498172314045744 0.0 62-63 0.0 0.0 0.0 0.6936370485429668 0.0 64-65 0.0 0.0 0.0 0.8218773543664487 0.0 66-67 0.0 0.0 0.0 1.0085989601257523 0.0 68-69 0.0 0.0 0.0 1.2305488638583242 0.0 70-71 0.0 0.0 0.0 1.3985518030377722 0.0 72-73 0.0 0.0 0.0 1.6360812180852546 0.0 74-75 0.0 0.0 0.0 1.8490787160622437 0.0 76-77 0.0 0.0 0.0 2.067540669500432 0.0 78-79 0.0 0.0 0.0 2.331462241775413 0.0 80-81 0.0 0.0 0.0 2.5958488740896453 0.0 82-83 0.0 0.0 0.0 2.9152288560453155 0.0 84-85 0.0 0.0 0.0 3.319366030154493 0.0 86-87 0.0 0.0 0.0 3.835931468752616 0.0 88-89 0.0 0.0 0.0 4.419116757973454 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGG 50 2.8216586E-5 47.5067 7 CATTCCG 240 0.0 41.56836 9 CGTGGAA 60 8.275185E-5 39.588913 9 GGTATCA 2465 0.0 38.58545 1 GTATCAA 3985 0.0 35.80168 1 CCTACAG 120 3.750756E-9 35.65495 3 GGACGTG 120 3.781679E-9 35.625874 6 TAGGACG 135 3.0377123E-10 35.214767 4 GTATGGT 55 0.0025628281 34.586594 1 ACGTGGA 70 2.0494976E-4 33.933357 8 GGCATTC 295 0.0 33.81833 7 GGTATGG 85 1.6282575E-5 33.53414 8 TAGGCAT 300 0.0 31.69329 5 AGTGGAC 90 2.4071454E-5 31.671131 8 GTAAGCG 30 0.003954433 31.660069 94-95 CGCCCTT 30 0.003956688 31.65638 88-89 GCATTCC 320 0.0 31.17627 8 GATGACC 155 1.3496901E-9 30.649483 9 GTATAGT 235 0.0 30.355255 1 CCTTTAG 345 0.0 30.325926 1 >>END_MODULE