##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430052 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19927822681908 33.0 31.0 34.0 31.0 34.0 2 32.41290355584906 34.0 31.0 34.0 31.0 34.0 3 32.50255783021588 34.0 31.0 34.0 31.0 34.0 4 36.146505538865064 37.0 35.0 37.0 35.0 37.0 5 36.01766763089115 37.0 35.0 37.0 35.0 37.0 6 36.12339670551468 37.0 35.0 37.0 35.0 37.0 7 36.08866137118302 37.0 35.0 37.0 35.0 37.0 8 36.09031000902216 37.0 35.0 37.0 35.0 37.0 9 37.72178248211844 39.0 37.0 39.0 35.0 39.0 10-11 37.70114776817687 39.0 37.0 39.0 35.0 39.0 12-13 37.64819254415745 39.0 37.0 39.0 35.0 39.0 14-15 38.81044152800126 40.0 38.0 41.0 35.0 41.0 16-17 38.82562806358301 40.0 38.0 41.0 35.5 41.0 18-19 38.93957591175021 40.0 38.0 41.0 35.5 41.0 20-21 38.9250462734739 40.0 38.0 41.0 35.0 41.0 22-23 38.779684782305395 40.0 38.0 41.0 35.0 41.0 24-25 38.71463334666505 40.0 38.0 41.0 34.5 41.0 26-27 38.591381972412634 40.0 38.0 41.0 34.5 41.0 28-29 38.465773906411314 40.0 38.0 41.0 34.0 41.0 30-31 38.3596018621004 40.0 37.5 41.0 34.0 41.0 32-33 38.13304786397924 40.0 37.0 41.0 34.0 41.0 34-35 37.94303130784184 40.0 37.0 41.0 34.0 41.0 36-37 37.735529656878704 40.0 36.0 41.0 33.0 41.0 38-39 37.554014398258815 40.0 36.0 41.0 33.0 41.0 40-41 37.32908462232474 40.0 36.0 41.0 33.0 41.0 42-43 37.0198708528271 40.0 36.0 41.0 33.0 41.0 44-45 36.539431278078 40.0 36.0 41.0 31.0 41.0 46-47 35.927950573419025 40.0 35.0 41.0 29.5 41.0 48-49 35.43412889604048 40.0 35.0 41.0 26.5 41.0 50-51 35.15328960218764 39.0 35.0 41.0 24.0 41.0 52-53 34.967958525945704 39.0 35.0 41.0 23.5 41.0 54-55 34.77394012817055 39.0 35.0 41.0 22.5 41.0 56-57 34.52617706695935 39.0 35.0 40.0 20.5 41.0 58-59 34.31272846074428 39.0 35.0 40.0 19.5 41.0 60-61 34.04967073749221 38.0 34.5 40.0 17.5 41.0 62-63 33.72261145163841 38.0 34.0 40.0 13.0 41.0 64-65 33.349189167821564 38.0 34.0 40.0 8.0 41.0 66-67 32.95029554565494 37.0 33.0 39.5 6.5 41.0 68-69 32.49210211788342 36.5 33.0 39.0 2.0 40.0 70-71 32.110129705244944 36.0 32.5 39.0 2.0 40.0 72-73 31.67724717010966 35.5 32.0 38.5 2.0 40.0 74-75 31.16744719243254 35.0 31.0 37.5 2.0 39.5 76-77 29.83694181168789 34.0 29.5 36.0 2.0 39.0 78-79 30.370030833480605 35.0 31.0 36.0 2.0 39.0 80-81 30.125263921572277 35.0 31.0 36.0 2.0 38.5 82-83 29.758032749527963 34.0 30.5 36.0 2.0 37.0 84-85 29.32376549812581 34.0 30.0 35.0 2.0 37.0 86-87 28.926494935496173 34.0 29.5 35.0 2.0 36.5 88-89 28.4873945476361 34.0 29.0 35.0 2.0 36.0 90-91 28.02565271176509 34.0 29.0 35.0 2.0 36.0 92-93 27.64451043129668 34.0 28.0 35.0 2.0 35.0 94-95 27.224946053035445 34.0 27.0 35.0 2.0 35.0 96-97 26.749258229237395 33.0 26.0 35.0 2.0 35.0 98-99 26.074194516012017 33.0 25.0 35.0 2.0 35.0 100-101 23.823509715104223 31.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3787151692039714 1101 2 0.2242190220410265 1101 3 0.2747467844610867 1101 4 0.22197638097624406 1101 5 0.09136281645838551 1101 6 0.1868825601513322 1101 7 0.19633357822633002 1101 8 0.22647999212790637 1101 9 0.3424117344377322 1101 10-11 0.37181640212765643 1101 12-13 0.4665768450101453 1101 14-15 0.6194445040487082 1101 16-17 0.42634201667751626 1101 18-19 0.5027264454577676 1101 20-21 0.42081674865384855 1101 22-23 0.36568810709359667 1101 24-25 0.16256122247244775 1101 26-27 0.337887758185893 1101 28-29 0.33787815696566526 1101 30-31 0.4428702432485494 1101 32-33 0.4658735547220445 1101 34-35 -0.00618194100290026 1101 36-37 0.2949750391795334 1101 38-39 0.2925806621721776 1101 40-41 0.42187669804841477 1101 42-43 0.32820000693825335 1101 44-45 0.44574141279669277 1101 46-47 0.46174023897014393 1101 48-49 0.4681754408205663 1101 50-51 0.5531437171626479 1101 52-53 0.6344927156021924 1101 54-55 0.5771181812787844 1101 56-57 0.8627959403470058 1101 58-59 1.0432824365117384 1101 60-61 0.9393722404879199 1101 62-63 0.8961483262859673 1101 64-65 0.7652007350139485 1101 66-67 1.2407377095002161 1101 68-69 1.105737248379171 1101 70-71 0.9210170331522036 1101 72-73 0.9210974104594136 1101 74-75 0.5286849265063296 1101 76-77 0.5249126084269804 1101 78-79 0.6820804651444838 1101 80-81 0.780580953244872 1101 82-83 0.9495476569641355 1101 84-85 1.0239684435656038 1101 86-87 1.1900751334713604 1101 88-89 1.0784605218472336 1101 90-91 1.2684213459748719 1101 92-93 1.5209612122787952 1101 94-95 1.6789886417663844 1101 96-97 1.8948069603547317 1101 98-99 2.127701001739343 1101 100-101 2.5043738414901817 1102 1 0.2747721321351335 1102 2 0.368003908258288 1102 3 0.22767722671098056 1102 4 0.1876447651182218 1102 5 0.17090339181261527 1102 6 0.15790016427327203 1102 7 0.14314551467974468 1102 8 0.2971469450404598 1102 9 0.31026274345448 1102 10-11 0.3931506657601105 1102 12-13 0.288075700026603 1102 14-15 0.3353544081563413 1102 16-17 0.43900839709829853 1102 18-19 0.3668938277391476 1102 20-21 0.372047605960276 1102 22-23 0.6115375417579756 1102 24-25 0.5197781968534443 1102 26-27 0.24212956439743039 1102 28-29 0.22976666419337022 1102 30-31 0.5733625861711218 1102 32-33 0.8376711788812017 1102 34-35 0.9007881463020979 1102 36-37 0.9749610846926302 1102 38-39 0.8765401583794201 1102 40-41 0.7073085715277259 1102 42-43 1.18349376677795 1102 44-45 0.985064441278162 1102 46-47 0.8608117288799733 1102 48-49 0.811259024619055 1102 50-51 0.7284335411214258 1102 52-53 0.6367558471321715 1102 54-55 0.7673911882918105 1102 56-57 0.769256152168964 1102 58-59 0.5084588355385051 1102 60-61 0.4995912185901048 1102 62-63 0.5236261422298654 1102 64-65 0.5251656698930063 1102 66-67 0.43338119025018074 1102 68-69 0.694366130588989 1102 70-71 0.5891417647966861 1102 72-73 0.4744196517646948 1102 74-75 0.4997740920995781 1102 76-77 0.5591994983654374 1102 78-79 0.764733964500433 1102 80-81 0.6443100215145137 1102 82-83 0.3997140373849142 1102 84-85 0.6834700179179762 1102 86-87 0.7054071786982092 1102 88-89 0.4946530937665088 1102 90-91 0.686420845044573 1102 92-93 0.8729184185189531 1102 94-95 0.9500366595136285 1102 96-97 1.3075198557318615 1102 98-99 1.571865163754513 1102 100-101 2.127611640259328 1103 1 0.355910395120425 1103 2 0.27298997969945304 1103 3 0.2875700297012145 1103 4 0.18762713403108933 1103 5 0.15750376559166313 1103 6 0.0641204118137324 1103 7 0.08589737475943338 1103 8 0.07210374887698379 1103 9 0.05152653978016275 1103 10-11 0.16779892557181597 1103 12-13 0.14477022381093718 1103 14-15 0.019149675972549574 1103 16-17 0.1894139121023528 1103 18-19 0.20766747984254152 1103 20-21 0.11987898224293758 1103 22-23 0.25720558364943 1103 24-25 0.240175601557425 1103 26-27 0.290271247813628 1103 28-29 0.2452026809781671 1103 30-31 0.07635987098370123 1103 32-33 0.18234130124094605 1103 34-35 0.5796378941775586 1103 36-37 0.5025801826862093 1103 38-39 0.45686739135660304 1103 40-41 0.6818404661643527 1103 42-43 0.5650393078760132 1103 44-45 0.6119818220038553 1103 46-47 0.680428076423965 1103 48-49 0.7834283535798718 1103 50-51 0.8383810004817676 1103 52-53 0.7356045371424003 1103 54-55 0.7761626541403928 1103 56-57 0.6976034140611276 1103 58-59 0.6830771225020769 1103 60-61 0.5625606462850143 1103 62-63 0.5091598352373836 1103 64-65 0.5229088907400055 1103 66-67 0.58941631137575 1103 68-69 0.6548989420421449 1103 70-71 0.8707719351129839 1103 72-73 1.129626111795023 1103 74-75 0.9447122069837377 1103 76-77 0.7705257087395694 1103 78-79 0.633629200580689 1103 80-81 0.8662942240604465 1103 82-83 0.8747572335483866 1103 84-85 1.074959492158957 1103 86-87 1.288149870612596 1103 88-89 1.369789734691789 1103 90-91 1.5947391919538418 1103 92-93 1.5808859067942826 1103 94-95 1.5963834982597085 1103 96-97 1.8724639651856485 1103 98-99 2.284954482841929 1103 100-101 2.8855368139255333 1104 1 0.05737873989540532 1104 2 0.08941510047240087 1104 3 0.04770860076655481 1104 4 -0.0011918336780354366 1104 5 -0.11464853545079734 1104 6 -0.06660041198489353 1104 7 0.0872004512913449 1104 8 -0.046094827273542194 1104 9 0.08712277674939628 1104 10-11 0.0544789408273445 1104 12-13 0.0799024384053908 1104 14-15 0.13660136679380486 1104 16-17 0.2329080641491359 1104 18-19 0.08773676537521169 1104 20-21 0.185716129458811 1104 22-23 0.15780627014829207 1104 24-25 0.0815498705632649 1104 26-27 0.012874858289087854 1104 28-29 0.0693668050564824 1104 30-31 0.060452802588081056 1104 32-33 0.12363929259614537 1104 34-35 0.1094656338165052 1104 36-37 0.11095801131575911 1104 38-39 -0.3135209890146129 1104 40-41 -0.16145663528492804 1104 42-43 0.16133984181956862 1104 44-45 0.016110349845199323 1104 46-47 0.2792711987224621 1104 48-49 0.4245737896337758 1104 50-51 0.5360513539015486 1104 52-53 0.4467347176661747 1104 54-55 0.2519762824139775 1104 56-57 0.18271338100748125 1104 58-59 0.03560699791524513 1104 60-61 0.09507399487387858 1104 62-63 0.15117928603829256 1104 64-65 0.4735985678312815 1104 66-67 0.31710509856110036 1104 68-69 0.3893414795453225 1104 70-71 0.4584912333586004 1104 72-73 0.4159374517390795 1104 74-75 0.6786462268159283 1104 76-77 0.25949723174999306 1104 78-79 0.42337303480392663 1104 80-81 0.4382176921509604 1104 82-83 0.4381312482954023 1104 84-85 0.661868340366432 1104 86-87 0.7379337506024228 1104 88-89 0.2569305940866542 1104 90-91 0.4378614904537166 1104 92-93 0.5823002215667188 1104 94-95 0.6130056696301587 1104 96-97 0.811473627021396 1104 98-99 1.0538490925546178 1104 100-101 1.5173382377750109 1105 1 0.15116790029641436 1105 2 0.20771867190097026 1105 3 0.18708046793447863 1105 4 0.13536048461770633 1105 5 -0.12541764572648617 1105 6 -0.07462829419040418 1105 7 0.06805101520112089 1105 8 0.10428111257608208 1105 9 0.20436901735089918 1105 10-11 0.09818194834614502 1105 12-13 0.12538724416980784 1105 14-15 0.21361029536523546 1105 16-17 0.30672542003885894 1105 18-19 0.2656816275305971 1105 20-21 0.22567931868186264 1105 22-23 -0.07310303436299392 1105 24-25 0.10947123647805768 1105 26-27 0.268494375832951 1105 28-29 0.16164829001888137 1105 30-31 0.055440271259755036 1105 32-33 0.06174627881168249 1105 34-35 -0.24718771831381758 1105 36-37 -0.28949624933587614 1105 38-39 -0.16664990505471167 1105 40-41 -0.12983461147539543 1105 42-43 -0.011063823593964628 1105 44-45 0.10345762428128324 1105 46-47 -0.20028707195422868 1105 48-49 -0.7656086978348924 1105 50-51 -0.6936831385796509 1105 52-53 -0.5863362409804367 1105 54-55 -0.4655989055559502 1105 56-57 -0.5894318257594335 1105 58-59 -0.30121161110731265 1105 60-61 -0.3634551404644668 1105 62-63 -0.4552391036860186 1105 64-65 -0.5161805675070639 1105 66-67 -0.42290899918325664 1105 68-69 -0.289308269828112 1105 70-71 -0.27022508869653805 1105 72-73 -0.4586513640503931 1105 74-75 -0.25389998245725565 1105 76-77 -0.1472031128615363 1105 78-79 0.04169618643300055 1105 80-81 0.02403771094795104 1105 82-83 0.022267369849288343 1105 84-85 0.06169603460201856 1105 86-87 0.2980058057402708 1105 88-89 0.019833946216976983 1105 90-91 0.08680807566674886 1105 92-93 0.37451513384742086 1105 94-95 0.4699331445675021 1105 96-97 0.5789587460690164 1105 98-99 0.8621197692463447 1105 100-101 1.0192766776246351 1106 1 -0.06458168442119927 1106 2 0.06383370512356379 1106 3 0.011488690525837342 1106 4 0.04257601617916862 1106 5 -0.18367138326801324 1106 6 -0.03331807824356048 1106 7 -0.04391949974905174 1106 8 -0.06191490071294936 1106 9 0.185286791437278 1106 10-11 0.18298261279797146 1106 12-13 -0.02335133556441349 1106 14-15 -0.06601491227596057 1106 16-17 -0.11394526102426283 1106 18-19 0.03590588002229822 1106 20-21 -0.08303642052079852 1106 22-23 -0.13859825861548103 1106 24-25 -0.11111638810666591 1106 26-27 0.0728911781585353 1106 28-29 0.013812949487324033 1106 30-31 0.0571429290670622 1106 32-33 -0.005991470357749051 1106 34-35 0.022751027533352897 1106 36-37 0.09686172408606097 1106 38-39 0.11169830923100932 1106 40-41 0.23689607789336975 1106 42-43 0.36507301195420894 1106 44-45 0.3612908884008945 1106 46-47 0.5322087752218536 1106 48-49 0.5675256460189289 1106 50-51 0.443043397965198 1106 52-53 0.3693479367604269 1106 54-55 0.4007216924506949 1106 56-57 0.25947286693159555 1106 58-59 0.41547235899235346 1106 60-61 0.6145660389375323 1106 62-63 0.7733998082741138 1106 64-65 0.4695441002670222 1106 66-67 0.5596387582918254 1106 68-69 -0.08621441600751467 1106 70-71 0.29364015216724937 1106 72-73 0.26151578711239765 1106 74-75 0.1012339461141778 1106 76-77 0.27221652999560675 1106 78-79 0.3955323575293441 1106 80-81 0.602936174403105 1106 82-83 0.5843572784871611 1106 84-85 0.4950639748013579 1106 86-87 0.6448288954412646 1106 88-89 0.7700986887418964 1106 90-91 0.7238876022448224 1106 92-93 0.8383232733823043 1106 94-95 0.693962421976142 1106 96-97 0.6302720766338012 1106 98-99 1.1747028257247543 1106 100-101 2.030587931777461 1107 1 0.0877464638410359 1107 2 0.1523290077571673 1107 3 0.19266328881116834 1107 4 0.06159136604312465 1107 5 -0.13519866906297295 1107 6 0.13903163170048316 1107 7 0.14356445733293555 1107 8 0.14184812613647324 1107 9 0.30223838032787853 1107 10-11 0.22397034317427256 1107 12-13 0.18300165996924278 1107 14-15 0.17040379504987158 1107 16-17 0.13624982085879367 1107 18-19 0.17401101888979298 1107 20-21 0.10809762126780242 1107 22-23 0.08569200269156596 1107 24-25 0.21386949412548262 1107 26-27 -0.1993070866394575 1107 28-29 -0.07163034842324834 1107 30-31 -0.09689731940564883 1107 32-33 -0.033834275429882155 1107 34-35 0.18028734869974272 1107 36-37 0.24886901398800632 1107 38-39 0.3689830774429268 1107 40-41 0.4189793470550356 1107 42-43 0.5950565840061657 1107 44-45 0.1288020542718229 1107 46-47 0.013121553085191806 1107 48-49 -0.08566496951575431 1107 50-51 0.04908580260609341 1107 52-53 -0.03448696236171145 1107 54-55 -0.1270030367448456 1107 56-57 -0.001823914217332856 1107 58-59 -0.09730807121453466 1107 60-61 0.20040976808173383 1107 62-63 0.03610579934141356 1107 64-65 -0.001501641300819756 1107 66-67 0.222298545755244 1107 68-69 0.32405305152162356 1107 70-71 0.35476752010195156 1107 72-73 0.448098773918705 1107 74-75 0.5436180520744429 1107 76-77 0.5003622253598436 1107 78-79 0.3841970733771838 1107 80-81 0.23949862627434726 1107 82-83 0.3762049092690276 1107 84-85 0.32932651394607504 1107 86-87 0.5731260359515566 1107 88-89 0.7281894531548545 1107 90-91 0.9493637560514863 1107 92-93 0.8196724186156352 1107 94-95 0.5156575808652022 1107 96-97 0.8525884173083718 1107 98-99 1.190111727619101 1107 100-101 1.6484379240768625 1108 1 0.037691400780126116 1108 2 0.10634512304576305 1108 3 0.054960788798716464 1108 4 -0.051056195485486455 1108 5 -0.19216526746141227 1108 6 -0.031260227437748256 1108 7 -0.08140178228356376 1108 8 -0.13503684937167293 1108 9 0.03205565069497851 1108 10-11 0.003414131512862184 1108 12-13 0.2346438347857358 1108 14-15 0.07269053157936156 1108 16-17 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-1.089871951251311 2316 96-97 -1.1681087824390595 2316 98-99 -1.6892145671489622 2316 100-101 -1.8784926643139137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 9.0 6 10.0 7 56.0 8 117.0 9 243.0 10 479.0 11 861.0 12 1448.0 13 2071.0 14 2718.0 15 4110.0 16 5908.0 17 8756.0 18 6922.0 19 3369.0 20 2371.0 21 2206.0 22 2247.0 23 2502.0 24 2735.0 25 3160.0 26 3587.0 27 4229.0 28 5377.0 29 6368.0 30 8133.0 31 9843.0 32 12720.0 33 17021.0 34 23526.0 35 34864.0 36 53240.0 37 95507.0 38 94005.0 39 9330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.39805509026614 23.42290154476084 16.575935231714126 19.603108133258885 2 14.287341995851666 23.381591063406283 41.872843284067976 20.458223656674075 3 16.924697478444468 28.809306781505494 33.774752820589136 20.491242919460902 4 10.482918344758309 20.527982662561737 41.5898542501837 27.399244742496254 5 9.604476414403226 41.61683957887099 36.40528667145751 12.373397335268276 6 24.827462725437854 42.67251402156018 18.913759266321282 13.586263986680681 7 23.903853487485236 37.90146307888349 22.67493233376429 15.519751099866994 8 24.64236882981593 36.030061480937185 21.531349697245915 17.796219992000967 9 26.635383628026375 16.848892692046544 23.211844149079646 33.30387953084743 10-11 22.27591082008687 29.476202877791525 29.548868508924503 18.6990177931971 12-13 23.635894329408455 28.845825160539444 29.86923142213008 17.649049087922016 14-15 21.03652581548278 29.699896756671286 29.617348599704226 19.646228828141712 16-17 16.815989694269533 32.96171160696846 30.9321895956768 19.29010910308521 18-19 16.7046078148689 30.09461646498563 36.192251169626 17.00852455051947 20-21 17.746923627840356 29.047650051621666 36.49942332555133 16.70600299498665 22-23 18.03102193812122 28.712209380061175 36.17412373663093 17.08264494518667 24-25 17.719546890423334 29.372713204612015 36.33038445484891 16.577355450115743 26-27 16.977968763988248 29.66386349446632 36.813987259576535 16.544180481968894 28-29 16.81101286500753 29.387211729073286 37.08156751948935 16.72020788642983 30-31 17.612986336536046 29.053230772092675 36.72520997460772 16.608572916763553 32-33 16.780180071247198 29.63339317105838 36.892399058718475 16.694027698975937 34-35 17.293722619590188 29.557007059611394 36.319677620380794 16.829592700417624 36-37 17.79808023215797 29.00579464808907 35.576511677657585 17.619613442095375 38-39 16.614037372224754 30.423762707765572 36.12132951364021 16.84087040636946 40-41 16.874703524224977 29.939867736924835 36.503376335884965 16.682052402965223 42-43 16.97039532203997 29.630490923022723 35.68258427292906 17.716529482008248 44-45 17.811683238306063 29.67420218950266 34.89752402035103 17.616590551840243 46-47 18.100718052700604 29.933124366355695 34.43537060634528 17.53078697459842 48-49 17.644377889185495 30.371792248379265 34.28841163394194 17.6954182284933 50-51 17.853189846809222 30.21192785988671 33.542571595993046 18.392310697311025 52-53 17.910392231637104 31.00938956219248 33.459093318947474 17.62112488722294 54-55 18.650884079134617 30.22994893640769 32.92892487420126 18.190242110256435 56-57 18.597750969650182 31.259591863309556 32.20982578850929 17.93283137853097 58-59 18.49125222066169 30.508636164928895 32.59012863560686 18.409982978802564 60-61 18.47369620417996 30.979858249700037 32.69034907406546 17.856096472054542 62-63 18.20105475616902 30.91684261438152 33.08611516746812 17.79598746198134 64-65 18.170709588607888 30.490615088407914 33.12483141573577 18.213843907248425 66-67 18.386032386781135 31.24145452177876 31.753485624994187 18.619027466445917 68-69 18.251164975398325 30.164724265902727 32.60861477216709 18.97549598653186 70-71 18.468929338777638 30.55363072372643 33.11901816524514 17.8584217722508 72-73 18.386170028473334 30.169758738197633 33.140216927507524 18.303854305821513 74-75 18.155499980816252 31.378799980932513 31.92001423085375 18.545685807397486 76-77 17.720182675583416 30.922655864872155 32.919507408406425 18.437654051138004 78-79 17.51485866825407 30.766860751723048 32.43119436719281 19.287086212830076 80-81 17.80935793810981 30.98392752504348 32.05031019504618 19.156404341800524 82-83 17.929343428236585 31.252150902681535 31.98357407941365 18.834931589668226 84-85 17.5349929020129 30.569594571812907 32.1268499237882 19.768562602385995 86-87 17.31848706668031 31.161812990057015 31.94974096155814 19.569958981704538 88-89 17.87237357342833 30.51665845060597 32.303070326379135 19.30789764958656 90-91 17.508673934478075 29.51312019794244 33.283956244302644 19.694249623276843 92-93 17.99845364120988 29.748106291819994 33.0911479679334 19.162292099036723 94-95 17.212918437770316 29.223675276478193 33.666858891482896 19.896547394268598 96-97 17.854468761917165 29.022420544492295 32.6360533144829 20.487057379107643 98-99 17.307558155757906 29.334592095839575 32.46409736496982 20.8937523834327 100-101 16.88160677761925 29.140111650254006 33.48693673423588 20.491344837890853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 819.0 1 580.0 2 396.0 3 456.0 4 371.0 5 380.5 6 710.0 7 1278.5 8 1844.0 9 2092.0 10 1852.0 11 1475.0 12 1372.5 13 1356.0 14 1304.0 15 1350.0 16 1447.5 17 1498.5 18 1545.5 19 1646.5 20 1803.0 21 2010.5 22 2227.5 23 2513.0 24 2879.0 25 3527.0 26 5227.0 27 7309.0 28 7664.5 29 8354.5 30 10193.5 31 11627.0 32 14206.5 33 17247.5 34 20050.5 35 22721.5 36 26395.5 37 29131.0 38 26225.5 39 21713.0 40 19173.0 41 16382.0 42 13349.0 43 13020.0 44 13563.5 45 12611.0 46 10755.5 47 9412.0 48 9923.5 49 8885.0 50 6769.0 51 5291.5 52 4151.5 53 3503.5 54 2913.5 55 2574.5 56 2269.5 57 1915.0 58 1607.0 59 1311.5 60 1038.0 61 784.5 62 568.5 63 411.5 64 289.0 65 245.5 66 218.5 67 165.0 68 117.5 69 103.5 70 87.0 71 62.0 72 50.0 73 37.0 74 22.5 75 17.5 76 17.5 77 15.0 78 9.0 79 5.5 80 4.5 81 3.0 82 3.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04929636416061313 2 0.0 3 0.0 4 0.0 5 0.015114451275659687 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0040692753434468385 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.487950294383005E-4 24-25 3.487950294383005E-4 26-27 8.138550686893678E-4 28-29 0.00220903518644257 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008254815696706445 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1626500981276684E-4 74-75 1.1626500981276684E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.1626500981276684E-4 86-87 0.0 88-89 0.0 90-91 0.006510840549514943 92-93 0.00220903518644257 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.011277705951838383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.10170435878654 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5780528482706 27.582235801690995 2 10.69685373678663 7.509555948757309 3 3.4262909800409593 3.6080595908572426 4 1.547261219685165 2.1724602359681655 5 0.9298405559632609 1.6319494148116047 6 0.6272182504303805 1.3209857757025534 7 0.4295688584247879 1.0555019349117836 8 0.36650752096498435 1.0292030916947712 9 0.2617834742766143 0.8270141508066344 >10 2.436052091998231 18.187355962203032 >50 0.4068902594959761 10.066503621274956 >100 0.27636572453491015 17.5542020235757 >500 0.013318830098067957 3.2080785737130197 >1k 0.003329707524516989 2.8333663940524274 >5k 6.659415049033979E-4 1.4135274799797894 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6047 1.406109028675602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4784 1.112423613888553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3690 0.8580357724182192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1402 0.3260070875149982 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.28577939411978087 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1016 0.2362504999395422 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 978 0.2274143591937719 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 976 0.22694929915452083 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 939 0.2183456884283761 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 865 0.20113846697608662 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 830 0.19299991628919294 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 824 0.19160473617143972 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 745 0.17323486462102258 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 683 0.1588180034042395 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 675 0.15695776324723523 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 657 0.1527722228939756 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 601 0.13975054179494573 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 590 0.13719271157906485 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 580 0.13486741138280953 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 555 0.12905416089217117 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 552 0.12835657083329458 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 551 0.12812404081366904 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.12719392073516692 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.1257987406174137 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 531 0.12347344042115836 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 504 0.11719512989126896 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 499 0.1160324797931413 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 496 0.1153348897342647 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 495 0.11510235971463915 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 477 0.11091681936137955 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 473 0.10998669928287742 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 458 0.10649874898849442 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 453 0.10533609889036674 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 438 0.10184814859598373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.3253001962553365E-4 0.0 6 0.0 0.0 0.0 2.3253001962553365E-4 0.0 7 0.0 0.0 0.0 2.3253001962553365E-4 0.0 8 0.0 0.0 0.0 2.3253001962553365E-4 0.0 9 0.0 0.0 0.0 2.3253001962553365E-4 0.0 10-11 0.0 0.0 0.0 2.3253001962553365E-4 0.0 12-13 0.0 0.0 0.0 2.3253001962553365E-4 0.0 14-15 0.0 0.0 0.0 2.3253001962553365E-4 0.0 16-17 0.0 0.0 0.0 2.3253001962553365E-4 0.0 18-19 0.0 0.0 0.0 2.3253001962553365E-4 0.0 20-21 0.0 0.0 0.0 2.3253001962553365E-4 0.0 22-23 0.0 0.0 0.0 3.487950294383005E-4 0.0 24-25 0.0 0.0 0.0 5.813250490638341E-4 0.0 26-27 0.0 0.0 0.0 6.97590058876601E-4 0.0 28-29 0.0 0.0 0.0 0.0032554202747574713 0.0 30-31 0.0 0.0 0.0 0.0056969854808255746 0.0 32-33 0.0 0.0 0.0 0.008022285677080911 0.0 34-35 0.0 0.0 0.0 0.010347585873336246 0.0 36-37 0.0 0.0 0.0 0.013603006148093719 0.0 38-39 0.0 0.0 0.0 0.017439751471915024 0.0 40-41 0.0 0.0 0.0 0.022787941923302298 0.0 42-43 0.0 0.0 0.0 0.033135527796638545 0.0 44-45 0.0 0.0 0.0 0.0529005794648089 0.0 46-47 0.0 0.0 0.0 0.09463971798759219 0.0 48-49 0.0 0.0 0.0 0.13219331615711588 0.0 50-51 0.0 0.0 0.0 0.17788546501353325 0.0 52-53 0.0 0.0 0.0 0.22590291406620594 0.0 54-55 0.0 0.0 0.0 0.2742691581483169 0.0 56-57 0.0 0.0 0.0 0.33414563820189186 0.0 58-59 0.0 0.0 0.0 0.4308781263661139 0.0 60-61 0.0 0.0 0.0 0.5584208421307191 0.0 62-63 0.0 0.0 0.0 0.7039846344163032 0.0 64-65 0.0 0.0 0.0 0.8331550603182871 0.0 66-67 0.0 0.0 0.0 1.0213881112051566 0.0 68-69 0.0 0.0 0.0 1.2468259652321114 0.0 70-71 0.0 0.0 0.0 1.416572879558751 0.0 72-73 0.0 0.0 0.0 1.657008919851553 0.0 74-75 0.0 0.0 0.0 1.8741919581818012 0.0 76-77 0.0 0.0 0.0 2.095676801875122 0.0 78-79 0.0 0.0 0.0 2.362504999395422 0.0 80-81 0.0 0.0 0.0 2.629798256954973 0.0 82-83 0.0 0.0 0.0 2.952317394175588 0.0 84-85 0.0 0.0 0.0 3.360291313608587 0.0 86-87 0.0 0.0 0.0 3.8819724126384716 0.0 88-89 0.0 0.0 0.0 4.477598057909276 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2655 0.0 40.822685 1 CAGTAGT 50 0.0016112955 38.016285 4 AGGTCAT 50 0.0016140603 38.00302 7 GTATCAA 4265 0.0 35.44372 1 TAGGCAT 335 0.0 34.044434 5 ATGGGTA 325 0.0 33.629787 5 CATTCCG 345 0.0 33.046104 9 CATGGGT 465 0.0 32.70218 4 GGCATTC 355 0.0 32.11523 7 GGATATG 75 3.0738336E-4 31.669188 7 TGGGACC 75 3.0759312E-4 31.665506 6 TTAGGCA 365 0.0 31.246264 4 AGGCATT 400 0.0 30.873865 6 CCTTTAG 380 0.0 30.023333 1 AGGTTAG 80 4.4686176E-4 29.710592 1 CATATAG 65 0.0058179107 29.253502 1 AGTCTGC 85 6.39763E-4 27.9434 8 GCATTCC 430 0.0 27.618477 8 GTACATG 2845 0.0 27.40267 1 TCAACGC 5585 0.0 26.80199 4 >>END_MODULE