FastQCFastQC Report
Thu 26 May 2016
SRR937651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009443
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.48898253789466073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.3898189397519226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26740.2648985628708109No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13700.13571841104450671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12640.12521757048193904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30250.036.1595151
TCTACGA550.002567935334.577052
CATTCCG2450.032.9647649
TCCGCCG300.003949817731.6696710-11
GTATCAA45900.029.7364671
GTACATG38950.027.3502121
ACATGGG40200.025.8992483
TACATGG40450.025.8579922
TCAACGC53700.025.3198344
CAACGCA53800.025.1844025
ATCAACG54750.024.8342483
AACGCAG54900.024.7467236
TATCAAC57600.023.6891842
CGTACAC1252.2424669E-422.819723
GAGTACT37000.021.95423912-13
ACGCAGA61850.021.8913367
CGCAGAG62150.021.8610238
GTGTAGC6550.021.7821161
GTAGCAC1754.018184E-621.7330673
AGTACTT38500.021.59242212-13
Produced by FastQC (version 0.11.5)