##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937651_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1009443 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75505798742475 33.0 31.0 34.0 30.0 34.0 2 31.9048366277244 33.0 31.0 34.0 30.0 34.0 3 31.924083875959315 33.0 31.0 34.0 30.0 34.0 4 35.5295138011755 37.0 35.0 37.0 33.0 37.0 5 35.53892790380438 37.0 35.0 37.0 33.0 37.0 6 35.60766977432109 37.0 35.0 37.0 33.0 37.0 7 35.56493828774879 37.0 35.0 37.0 33.0 37.0 8 35.5563226452608 37.0 35.0 37.0 33.0 37.0 9 37.19482922760374 39.0 37.0 39.0 34.0 39.0 10-11 37.079928237651856 39.0 37.0 39.0 33.0 39.0 12-13 36.998894935127595 39.0 37.0 39.0 33.0 39.0 14-15 38.17231334508239 40.0 38.0 41.0 33.0 41.0 16-17 38.089565235481345 40.0 38.0 41.0 33.0 41.0 18-19 38.06837335045168 40.0 38.0 41.0 33.0 41.0 20-21 37.998863234476836 40.0 38.0 41.0 33.0 41.0 22-23 37.91371677251712 40.0 38.0 41.0 33.0 41.0 24-25 37.97185378471097 40.0 38.0 41.0 33.0 41.0 26-27 37.90445176201133 40.0 38.0 41.0 33.0 41.0 28-29 37.80541248985827 40.0 37.5 41.0 33.0 41.0 30-31 37.59380866477849 40.0 37.0 41.0 32.5 41.0 32-33 37.44779249546532 40.0 37.0 41.0 32.5 41.0 34-35 37.43150083759063 40.0 37.0 41.0 32.5 41.0 36-37 37.30179911099488 40.0 37.0 41.0 32.0 41.0 38-39 37.16646259372743 40.0 36.5 41.0 31.0 41.0 40-41 36.98161956643416 40.0 36.0 41.0 31.0 41.0 42-43 36.74362891218226 39.0 36.0 41.0 30.5 41.0 44-45 36.50482741472277 39.0 36.0 41.0 30.0 41.0 46-47 36.1658523562004 39.0 36.0 41.0 29.5 41.0 48-49 35.88413560745877 39.0 35.0 41.0 28.5 41.0 50-51 35.035189208305965 38.5 34.0 39.5 27.0 40.5 52-53 35.151276991370494 38.5 35.0 40.0 27.5 40.5 54-55 35.49940808941169 39.0 35.0 40.0 27.5 41.0 56-57 35.329879448369056 39.0 35.0 40.0 26.5 41.0 58-59 35.13954775059116 39.0 35.0 40.0 26.0 41.0 60-61 34.87593999859328 38.0 34.5 40.0 26.0 41.0 62-63 34.54815180252872 38.0 34.0 40.0 25.0 41.0 64-65 34.21998914252712 38.0 34.0 40.0 23.5 41.0 66-67 33.82990867240646 37.5 33.5 40.0 22.5 41.0 68-69 33.336790685556295 37.0 33.0 39.0 22.0 41.0 70-71 32.923333957439894 36.0 32.0 39.0 20.5 40.0 72-73 32.46513077013759 36.0 32.0 39.0 19.0 40.0 74-75 31.92681706644159 35.0 31.0 38.0 17.5 40.0 76-77 31.43230969950755 35.0 31.0 37.0 12.5 39.0 78-79 30.8657680522823 35.0 30.5 37.0 7.5 39.0 80-81 30.285967607878803 34.0 30.0 36.0 7.0 38.5 82-83 29.770542764673188 34.0 29.0 36.0 2.0 37.0 84-85 29.25205385544305 34.0 29.0 35.0 2.0 37.0 86-87 28.72657594336679 34.0 29.0 35.0 2.0 36.0 88-89 28.23048602050834 34.0 27.5 35.0 2.0 36.0 90-91 27.6869258591124 33.0 26.5 35.0 2.0 35.5 92-93 27.099159635561392 33.0 26.0 35.0 2.0 35.0 94-95 26.393318394401668 32.0 25.0 34.5 2.0 35.0 96-97 25.58136814064786 32.0 22.0 34.0 2.0 35.0 98-99 24.554581090759953 32.0 10.0 34.0 2.0 35.0 100-101 21.630158414095696 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11602479177000902 1101 2 0.1589463636500028 1101 3 0.21297850452884504 1101 4 0.11840156584317896 1101 5 0.049563770081114455 1101 6 0.09834522263354728 1101 7 0.19727102936406737 1101 8 0.19851011481987513 1101 9 0.3064182082822313 1101 10-11 0.2862471461342153 1101 12-13 0.13141821345826799 1101 14-15 0.39142444060486525 1101 16-17 0.3998855065947211 1101 18-19 0.527330877640452 1101 20-21 0.38847056417165504 1101 22-23 0.4040020698003701 1101 24-25 0.3535323879204597 1101 26-27 0.3484491481920031 1101 28-29 0.4635878224322596 1101 30-31 0.36285137574680704 1101 32-33 0.4298324704623866 1101 34-35 0.5641608566155085 1101 36-37 0.6766380427650063 1101 38-39 0.6926806072104128 1101 40-41 0.528070076174977 1101 42-43 0.38856110716776016 1101 44-45 0.6624439950505092 1101 46-47 0.6177424420308242 1101 48-49 0.6719096875478812 1101 50-51 0.586297621466926 1101 52-53 0.6224056298884761 1101 54-55 0.6732057817173356 1101 56-57 0.6412975696682679 1101 58-59 0.591467636342415 1101 60-61 0.7630718195791033 1101 62-63 0.8806972409147633 1101 64-65 0.8391930530317282 1101 66-67 0.8016753296077965 1101 68-69 0.8628850581361931 1101 70-71 0.9864578382613374 1101 72-73 0.9842845480623836 1101 74-75 0.9745243812011317 1101 76-77 0.9943966546000524 1101 78-79 0.8380254652544927 1101 80-81 0.9425732877264181 1101 82-83 0.750430711845901 1101 84-85 0.8378223050173865 1101 86-87 1.0094898994680648 1101 88-89 0.8147879843728312 1101 90-91 1.3301981553290396 1101 92-93 1.045088415139272 1101 94-95 1.1160696709514326 1101 96-97 1.013367417680083 1101 98-99 1.2302386170100519 1101 100-101 1.5859529191176769 1102 1 0.21245709509886623 1102 2 0.32083619795363205 1102 3 0.37394110514661705 1102 4 0.2581870676976479 1102 5 0.19498157067133093 1102 6 0.2402177179543159 1102 7 0.2732352949835075 1102 8 0.14187135128456418 1102 9 0.44117425683138833 1102 10-11 0.24972476047390302 1102 12-13 0.41011939733186153 1102 14-15 0.44914401987616515 1102 16-17 0.5282973246617146 1102 18-19 0.41308305649607746 1102 20-21 0.5215156799753942 1102 22-23 0.41340789386136834 1102 24-25 0.5761919123041181 1102 26-27 0.4837879473239113 1102 28-29 0.7346185158767398 1102 30-31 0.5450097346293035 1102 32-33 0.5796352538858613 1102 34-35 0.60260947696068 1102 36-37 0.6433537982738642 1102 38-39 0.896438056657523 1102 40-41 0.6993743466171836 1102 42-43 0.7309356866113035 1102 44-45 0.81678677870363 1102 46-47 1.0084235999533675 1102 48-49 0.8804425340117632 1102 50-51 0.7812377388208418 1102 52-53 0.5863282015696711 1102 54-55 0.49752514051944985 1102 56-57 0.5997902264367951 1102 58-59 0.6301703129761194 1102 60-61 0.5757045891084189 1102 62-63 0.7562656380348827 1102 64-65 0.7195267997447772 1102 66-67 0.6522198999799613 1102 68-69 0.5263968337169374 1102 70-71 0.6279815444402175 1102 72-73 0.7343950697246342 1102 74-75 0.5956403342136376 1102 76-77 0.7183609087367131 1102 78-79 0.8956847599827533 1102 80-81 0.8574027111765332 1102 82-83 1.0196141146499933 1102 84-85 1.0651768470271001 1102 86-87 0.9743798945304754 1102 88-89 1.259146786490252 1102 90-91 1.395384405860117 1102 92-93 1.372177992228849 1102 94-95 1.4239787159294401 1102 96-97 1.5746480910297223 1102 98-99 1.3685772898314816 1102 100-101 1.7769831532513614 1103 1 0.34719372312408225 1103 2 0.34452950688184103 1103 3 0.3719777441228551 1103 4 0.26363911006244223 1103 5 0.1624179374365582 1103 6 0.17877468671262875 1103 7 0.3408500068935183 1103 8 0.29108678909059904 1103 9 0.3229267325435927 1103 10-11 0.4606570049631884 1103 12-13 0.3869697282090456 1103 14-15 0.4661963188076612 1103 16-17 0.43104837674162155 1103 18-19 0.5010197283253746 1103 20-21 0.5443289964128724 1103 22-23 0.38223269322308084 1103 24-25 0.534167382024556 1103 26-27 0.5047787015361394 1103 28-29 0.4443930813560115 1103 30-31 0.5756452065399387 1103 32-33 0.6086945614686528 1103 34-35 0.5959450004975437 1103 36-37 0.5292363720758999 1103 38-39 0.33285566589392346 1103 40-41 0.3306496708455242 1103 42-43 0.27033588372962214 1103 44-45 0.22165372383904014 1103 46-47 0.24048739745339986 1103 48-49 -0.041480621132869544 1103 50-51 0.2434371724967832 1103 52-53 0.1996047460542627 1103 54-55 0.260498185371695 1103 56-57 0.2618770210739143 1103 58-59 0.1530853174347513 1103 60-61 0.2850684914253421 1103 62-63 0.22800027923261013 1103 64-65 0.42942651971161894 1103 66-67 0.28849358155564886 1103 68-69 0.12229645981791037 1103 70-71 0.2214797688109229 1103 72-73 0.17674578388743356 1103 74-75 0.17720977148777095 1103 76-77 0.3507391568867817 1103 78-79 0.4705193213944945 1103 80-81 0.6641407159011159 1103 82-83 0.5112678747601294 1103 84-85 0.5442408479031826 1103 86-87 0.532099579766939 1103 88-89 0.5610506991324193 1103 90-91 0.7015201046132695 1103 92-93 0.5494827903390167 1103 94-95 0.6396629198686803 1103 96-97 1.139509404213186 1103 98-99 1.1383204102457434 1103 100-101 1.1332275799655847 1104 1 0.18760730408528303 1104 2 0.1019882355711843 1104 3 0.09182702669633258 1104 4 0.09377102397125725 1104 5 0.2341388932338262 1104 6 0.15822822755965404 1104 7 0.15001117714732004 1104 8 0.06996947442578971 1104 9 0.17710786345464413 1104 10-11 0.07557904268593774 1104 12-13 0.33954629227600464 1104 14-15 0.41613195292013216 1104 16-17 0.22547048196418018 1104 18-19 0.27871634562075087 1104 20-21 0.294104800624865 1104 22-23 0.2442883998496299 1104 24-25 0.3332891613194775 1104 26-27 0.4518974240540814 1104 28-29 0.5334000145504874 1104 30-31 0.4428236663871985 1104 32-33 0.4822493423835681 1104 34-35 0.554385610310085 1104 36-37 0.5350124270014618 1104 38-39 0.4595371343040071 1104 40-41 0.5341507411996034 1104 42-43 0.47769288056677084 1104 44-45 0.41390643347415335 1104 46-47 0.356889609518511 1104 48-49 0.47871387473999505 1104 50-51 0.2630217593979651 1104 52-53 0.39134035895251174 1104 54-55 0.36686341718531423 1104 56-57 0.5035974465155633 1104 58-59 0.486941774273447 1104 60-61 0.5679517456874734 1104 62-63 0.551648595594564 1104 64-65 0.6233679298790165 1104 66-67 0.8701020537457325 1104 68-69 0.7761393684810187 1104 70-71 0.7560083308721701 1104 72-73 0.7046509273727324 1104 74-75 1.0061592334178862 1104 76-77 1.0358067531222162 1104 78-79 0.7501252189490764 1104 80-81 0.7963294453618879 1104 82-83 0.8280938891858014 1104 84-85 0.7244448419632015 1104 86-87 0.969773150699595 1104 88-89 0.9616475902841621 1104 90-91 0.9662049287280574 1104 92-93 0.70472511464666 1104 94-95 0.7250598187346817 1104 96-97 0.848265816694866 1104 98-99 0.935672730310543 1104 100-101 1.274222623551175 1105 1 0.3151334487998092 1105 2 0.39339581047226346 1105 3 0.3352025213260639 1105 4 0.3301687062776466 1105 5 0.34749742912980963 1105 6 0.3368778883849828 1105 7 0.36808989393646385 1105 8 0.31585344842239493 1105 9 0.5036694801872628 1105 10-11 0.3506373383331933 1105 12-13 0.2619777507271053 1105 14-15 0.45873193878785656 1105 16-17 0.5635322400195761 1105 18-19 0.5406051245919201 1105 20-21 0.5925431838922464 1105 22-23 0.6351253840214781 1105 24-25 0.5712325615455924 1105 26-27 0.4739791086216272 1105 28-29 0.48119007955896365 1105 30-31 0.512416303470296 1105 32-33 0.4921331043960038 1105 34-35 0.5153186939731782 1105 36-37 0.5833448181829226 1105 38-39 0.5109521286511054 1105 40-41 0.585459743460774 1105 42-43 0.5310952265249398 1105 44-45 0.39488968672457503 1105 46-47 0.4583858644648089 1105 48-49 0.6093667859892946 1105 50-51 0.4238647497880166 1105 52-53 0.714139256634823 1105 54-55 0.7035119474283889 1105 56-57 0.6106582859728888 1105 58-59 0.8979967488353466 1105 60-61 1.0369755585761098 1105 62-63 0.8048548561937707 1105 64-65 0.801116657973985 1105 66-67 1.0259899141188242 1105 68-69 0.8852493882662102 1105 70-71 1.1080963749648802 1105 72-73 1.146805746479572 1105 74-75 1.0113086822590382 1105 76-77 1.0370609928056567 1105 78-79 0.8830039640027785 1105 80-81 0.9944816499972724 1105 82-83 0.9803250225945099 1105 84-85 0.7830849635007908 1105 86-87 0.9735535350975582 1105 88-89 0.9980028757561783 1105 90-91 0.6856527099603902 1105 92-93 0.6325445041322446 1105 94-95 0.890849106467865 1105 96-97 1.2490660569435938 1105 98-99 1.4404335415033067 1105 100-101 1.6787060887857699 1106 1 0.24842379080996935 1106 2 0.3473634513179462 1106 3 0.22197166883212915 1106 4 0.33616021276344554 1106 5 0.18619376635636797 1106 6 0.17116270480985207 1106 7 0.20082527688659013 1106 8 0.22189994888504572 1106 9 0.3606578616185061 1106 10-11 0.16270687952630425 1106 12-13 -0.04828375436201782 1106 14-15 0.174171388673507 1106 16-17 0.10498395007038397 1106 18-19 0.20635740727841778 1106 20-21 0.1306892540051976 1106 22-23 0.2682173549008624 1106 24-25 0.21020965175316775 1106 26-27 0.25173033507460474 1106 28-29 0.393523242672849 1106 30-31 0.4156149015603532 1106 32-33 0.4446009591071558 1106 34-35 0.5726248183817759 1106 36-37 0.5804707282023003 1106 38-39 0.650675565687358 1106 40-41 0.6819998148656481 1106 42-43 0.661983073728976 1106 44-45 0.6247099524415063 1106 46-47 0.7069553663765049 1106 48-49 0.574187553635376 1106 50-51 0.6101399335377309 1106 52-53 0.5290659749783657 1106 54-55 0.5060131913397754 1106 56-57 0.5733393078133489 1106 58-59 0.6266756713025643 1106 60-61 0.6438733745296048 1106 62-63 0.542761091463241 1106 64-65 0.6987209282999274 1106 66-67 0.6394291763266153 1106 68-69 0.499136022111081 1106 70-71 0.40867555756234 1106 72-73 0.5744253802022214 1106 74-75 0.4841674088429464 1106 76-77 0.46266745416147614 1106 78-79 0.7152052288642459 1106 80-81 0.42742773407695367 1106 82-83 0.27090435795301815 1106 84-85 0.34447635476111316 1106 86-87 0.5306665258969794 1106 88-89 0.5826608655953862 1106 90-91 0.545855074701393 1106 92-93 0.5784065347193739 1106 94-95 0.8171882752731854 1106 96-97 1.1843057390260299 1106 98-99 0.9760994269063339 1106 100-101 1.2733470130337494 1107 1 0.4568629240117339 1107 2 0.3690344785462756 1107 3 0.356650883631044 1107 4 0.2825242328798865 1107 5 0.3617819064857528 1107 6 0.21946851410652357 1107 7 0.2770601086674773 1107 8 0.33151504092825235 1107 9 0.3975512132083452 1107 10-11 0.3083539247368634 1107 12-13 0.3963526476496284 1107 14-15 0.37769551532907286 1107 16-17 0.619513620103362 1107 18-19 0.6613418399661342 1107 20-21 0.6314456561025565 1107 22-23 0.4961150927657485 1107 24-25 0.558208721785725 1107 26-27 0.7695620323937646 1107 28-29 0.5866538045317569 1107 30-31 0.6261293192700137 1107 32-33 0.6727815442152476 1107 34-35 0.6311056286962682 1107 36-37 0.5954873454778351 1107 38-39 0.5398931930704052 1107 40-41 0.6045548468681048 1107 42-43 0.7979051021722725 1107 44-45 0.797374266333378 1107 46-47 0.5930772092548651 1107 48-49 0.5394771613562455 1107 50-51 0.3074521347535395 1107 52-53 0.32388172785558567 1107 54-55 0.5328290384994077 1107 56-57 0.8098606724227295 1107 58-59 0.5214093556236179 1107 60-61 0.5375115614787731 1107 62-63 0.681857651655065 1107 64-65 0.8180749651943557 1107 66-67 0.8976563883741946 1107 68-69 0.9238890128992594 1107 70-71 0.7279672394963299 1107 72-73 0.6888654819415265 1107 74-75 0.5646079862798778 1107 76-77 0.7485161994553948 1107 78-79 0.8348946111644366 1107 80-81 0.8688724620829014 1107 82-83 0.8389532262426265 1107 84-85 0.6477421690290406 1107 86-87 0.6609937501584149 1107 88-89 0.9971493942111707 1107 90-91 1.1540811082187936 1107 92-93 1.049112560008485 1107 94-95 1.177325134768818 1107 96-97 1.2977114126845954 1107 98-99 1.5224574472327674 1107 100-101 1.6017546259101572 1108 1 0.14735361068702346 1108 2 0.10766704242136171 1108 3 0.32414364036356247 1108 4 0.29551669292352756 1108 5 0.3583801317644131 1108 6 0.22743079306364677 1108 7 0.31512616108186364 1108 8 0.41831344905230594 1108 9 0.39254229321382894 1108 10-11 0.4113465200783679 1108 12-13 0.5207546794368909 1108 14-15 0.5011717232007058 1108 16-17 0.6764822329330755 1108 18-19 0.5979644575158147 1108 20-21 0.6004943382047756 1108 22-23 0.543522495877987 1108 24-25 0.5966637555425365 1108 26-27 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3464.0 3 414.0 4 394.0 5 454.0 6 560.0 7 633.0 8 863.0 9 1051.0 10 1472.0 11 2023.0 12 2928.0 13 4090.0 14 4716.0 15 6309.0 16 8210.0 17 11143.0 18 9149.0 19 6007.0 20 5740.0 21 6085.0 22 6642.0 23 7606.0 24 8592.0 25 9934.0 26 11843.0 27 13865.0 28 16284.0 29 19786.0 30 24439.0 31 30364.0 32 38274.0 33 49919.0 34 66607.0 35 93675.0 36 135802.0 37 204558.0 38 176743.0 39 18805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.286793713081046 22.089298262877342 15.309082993651653 22.314825030389958 2 14.063265368981748 21.959066390185793 42.09381604218353 21.883852198648928 3 17.306077758668142 26.31655295273693 32.195498537963765 24.181870750631166 4 11.103504670343591 18.908209690031466 40.940050096526726 29.048235543098215 5 11.458901598146973 39.440136810840734 36.62621366458887 12.474747926423433 6 27.830779111331257 40.23805435087952 19.13666336780102 12.794503169988197 7 25.649455391539387 35.0709385649098 22.681679258224715 16.597926785326095 8 23.579732702279895 37.27973349639077 21.551176475259474 17.589357326069866 9 25.006253548640693 17.470826699277765 23.231337624127978 34.29158212795356 10-11 22.44260509865858 28.522389645043546 30.043837226501267 18.991168029796608 12-13 23.188274892082116 26.911429419372514 29.720172827423696 20.18012286112167 14-15 20.807535876559324 28.530767111933876 28.186729333290323 22.47496767821648 16-17 18.990802920432607 31.613285883748514 29.51051484784864 19.885396347970243 18-19 18.694867155093092 30.411790922256422 32.190335430039916 18.703006492610573 20-21 19.766918483501225 29.32173219148137 32.129002738836846 18.78234658618056 22-23 19.535688338911186 29.515629900472035 31.4873813465455 19.461300414071278 24-25 19.1681608552532 29.69957256198298 32.09457531380442 19.037691268959406 26-27 19.266949661408734 29.731965536441113 32.04081754183634 18.96026726031381 28-29 18.78505002235955 29.80719168512591 32.25785797987789 19.149900312636646 30-31 19.39690430998197 29.51487884876546 32.14344568247338 18.944771158779194 32-33 19.027059760797968 29.66398046276832 31.933396703588528 19.375563072845186 34-35 19.434906093988406 29.776281423197997 31.819341491477033 18.969470991336564 36-37 19.56586246935879 29.344402211129083 31.53027400930897 19.55946131020315 38-39 18.894850205988785 30.258047902129654 31.507030114602504 19.340071777279064 40-41 19.41564505612614 29.710730309703553 31.84467483955099 19.02894979461931 42-43 19.213861977721823 29.73813264677187 31.497479487024243 19.550525888482063 44-45 19.61347847535368 29.90164500686371 31.169470546605222 19.31540597117739 46-47 19.600052013487772 29.987860210773743 30.671579492789085 19.740508282949403 48-49 19.387264876668816 29.941932602114246 30.931857660429795 19.738944860787136 50-51 19.41475752460638 30.138329768917664 30.434683344393033 20.012229362082923 52-53 19.54629469199343 30.547064702205624 30.387651240480988 19.518989365319953 54-55 19.86783657828147 29.965395931974943 30.377389296013412 19.78937819373018 56-57 19.848329305221394 30.428858713746372 30.021896191313076 19.700915789719158 58-59 19.791834126223325 30.143414845202866 30.220967226552187 19.843783802021623 60-61 20.038262338140438 30.17416012133607 30.153322229713943 19.634255310809557 62-63 19.82330919402496 29.982211835455757 30.472887314334486 19.7215916561848 64-65 19.70697493722274 29.94285668922067 30.284763917301223 20.065404456255372 66-67 19.698732563129965 30.07985670942726 30.043240212653533 20.178170514789244 68-69 19.700619809387895 29.82364385993727 30.26209040156364 20.213645929111195 70-71 19.73102046993269 29.946189208567052 30.54979296862402 19.772997352876235 72-73 19.98140387819441 29.609709676234967 30.41497930511631 19.993907140454308 74-75 19.670620621762993 30.05210964162341 30.10738496134201 20.169884775271587 76-77 19.565793417184867 29.960432846989143 30.299586961397463 20.174186774428524 78-79 19.400971304005648 29.853877648644396 30.20277100021883 20.54238004713112 80-81 19.454662912930047 29.800378483031274 30.101706150696582 20.643252453342093 82-83 19.51938825278235 30.085047033624363 30.167161753097165 20.228402960496123 84-85 19.613325864916582 29.74530152078861 30.084059378874468 20.557313235420338 86-87 19.440034529632975 29.788952501910043 30.195171804768663 20.57584116368832 88-89 19.98329982714378 29.464542645474083 30.14252203370994 20.409635493672194 90-91 19.626875410286704 29.252406146994158 30.598171343170126 20.522547099549012 92-93 19.788298624462 29.378465202237575 30.640090296813565 20.19314587648686 94-95 19.772221750867914 29.196430210536604 30.515577267623566 20.515770770971915 96-97 19.98748692006067 29.10397516039516 30.099464596964214 20.80907332257996 98-99 19.720907477415032 29.02273456885991 30.14380846576021 21.112549487964856 100-101 19.971794277800072 28.537930644480415 30.95491201709606 20.535363060623453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1770.0 1 1028.5 2 308.0 3 322.5 4 288.5 5 297.5 6 476.0 7 872.5 8 1278.0 9 1403.5 10 1207.5 11 980.0 12 972.5 13 1017.5 14 1021.5 15 1063.5 16 1139.5 17 1216.0 18 1369.5 19 1593.0 20 1903.5 21 2374.0 22 2867.5 23 3503.5 24 4409.0 25 6048.5 26 9231.5 27 12706.0 28 14314.0 29 16525.0 30 20584.5 31 24553.0 32 29478.5 33 35083.0 34 41013.5 35 45563.5 36 48418.0 37 50319.0 38 48135.0 39 44892.0 40 43729.0 41 42880.5 42 40863.5 43 39682.5 44 39043.5 45 37176.5 46 34937.0 47 32485.5 48 31712.0 49 29735.5 50 25757.0 51 22236.0 52 19352.5 53 16886.5 54 14624.0 55 12618.5 56 10447.0 57 8469.0 58 6744.0 59 5386.0 60 4181.5 61 3102.0 62 2246.0 63 1463.0 64 911.5 65 608.0 66 407.5 67 289.0 68 205.0 69 155.0 70 120.0 71 73.5 72 57.0 73 48.0 74 36.0 75 33.5 76 24.0 77 16.5 78 13.5 79 10.0 80 5.5 81 2.5 82 2.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3322624457250187 2 0.29560856828964094 3 0.2942216648191131 4 0.29580669735685916 5 0.3061094088522086 6 0.20070474509209535 7 0.20030848695765882 8 0.20080380962570446 9 0.1996150352223949 10-11 0.20605422990698832 12-13 0.19456274400832935 14-15 0.1976832768170169 16-17 0.20387481016758746 18-19 0.1971384218821667 20-21 0.1880740170569314 22-23 0.1869347749204264 24-25 0.19104595306520528 26-27 0.1868357103868173 28-29 0.20243837443025509 30-31 0.20872897231443477 32-33 0.21155231152229498 34-35 0.20862990778082566 36-37 0.17960399943335087 38-39 0.17261994981390727 40-41 0.17851428956365045 42-43 0.1892132591934364 44-45 0.19515713120998412 46-47 0.19931784162156752 48-49 0.1974356154829941 50-51 0.20124960002694553 52-53 0.22918579850471993 54-55 0.18847027519136791 56-57 0.1717283690114251 58-59 0.17207509487905706 60-61 0.16479385165878607 62-63 0.17341246608278033 64-65 0.16974707833924255 66-67 0.16845923940232385 68-69 0.177771305561582 70-71 0.17291714341473466 72-73 0.16900409433717406 74-75 0.17435357915206703 76-77 0.17722645062673179 78-79 0.18113949970429238 80-81 0.17197603034544792 82-83 0.16905362660397863 84-85 0.16281256098660352 86-87 0.15979109271152506 88-89 0.1653387065936363 90-91 0.174700305019699 92-93 0.16400133538991304 94-95 0.16925175567119688 96-97 0.16816204580149646 98-99 0.17321433701556205 100-101 0.1477547518780159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1009443.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.737619728694945 #Duplication Level Percentage of deduplicated Percentage of total 1 79.48651016870465 45.09875387513009 2 12.62293435322247 14.32390498386833 3 3.805665833307174 6.477732625940083 4 1.4705996759274018 3.3375330074364378 5 0.706683689881268 2.0047775232477187 6 0.3964743707608784 1.3497007248242596 7 0.23958759734353194 0.9515540992852299 8 0.17449311553038122 0.7920259227390404 9 0.11774144339327622 0.6012332317399832 >10 0.7717189925642837 8.836415029144243 >50 0.12414224996125117 4.933883423393289 >100 0.07836446181243753 8.261103420748698 >500 0.004207487661516481 1.6210275171740007 >1k 8.765599294826002E-4 1.4103546153285975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4936 0.48898253789466073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3935 0.3898189397519226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2674 0.2648985628708109 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1370 0.13571841104450671 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1264 0.12521757048193904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.9064533609129E-5 0.0 10-11 0.0 0.0 0.0 9.9064533609129E-5 0.0 12-13 0.0 0.0 0.0 9.9064533609129E-5 0.0 14-15 0.0 0.0 0.0 2.97193600827387E-4 0.0 16-17 0.0 0.0 0.0 3.467258676319515E-4 0.0 18-19 0.0 0.0 0.0 3.96258134436516E-4 0.0 20-21 0.0 0.0 0.0 3.96258134436516E-4 0.0 22-23 0.0 0.0 0.0 5.943872016547739E-4 0.0 24-25 0.0 0.0 0.0 9.906453360912898E-4 0.0 26-27 0.0 0.0 0.0 0.0014364357373323703 0.0 28-29 0.0 0.0 0.0 0.0028728714746647406 0.0 30-31 0.0 0.0 0.0 0.006884985085834465 0.0 32-33 0.0 0.0 0.0 0.012878389369186768 0.0 34-35 0.0 0.0 0.0 0.020308229389871445 0.0 36-37 0.0 0.0 0.0 0.027688537143751555 0.0 38-39 0.0 0.0 0.0 0.032988489691839956 0.0 40-41 0.0 0.0 0.0 0.0379417163722964 0.0 42-43 0.0 0.0 0.0 0.042894943052752856 0.0 44-45 0.0 0.0 0.0 0.04903694413651885 0.0 46-47 0.0 0.0 0.0 0.0601321719007413 0.0 48-49 0.0 0.0 0.0 0.07246570633507786 0.0 50-51 0.0 0.0 0.0 0.08321420823166836 0.0 52-53 0.0 0.0 0.0 0.1065934381634228 0.0 54-55 0.0 0.0 0.0 0.1517668654891856 0.0 56-57 0.0 0.0 0.0 0.22973065343957014 0.0 58-59 0.0 0.0 0.0 0.3174522979504539 0.0 60-61 0.0 0.0 0.0 0.3971992475058027 0.0 62-63 0.0 0.0 0.0 0.4867535858884553 0.0 64-65 0.0 0.0 0.0 0.6090982848957296 0.0 66-67 0.0 0.0 0.0 0.7762201530943302 0.0 68-69 0.0 0.0 0.0 0.9788071243249992 0.0 70-71 0.0 0.0 0.0 1.16351294723922 0.0 72-73 0.0 0.0 0.0 1.346881398949718 0.0 74-75 0.0 0.0 0.0 1.5588795008732537 0.0 76-77 0.0 0.0 0.0 1.8199145469333087 0.0 78-79 0.0 0.0 0.0 2.0565301854587132 0.0 80-81 0.0 0.0 0.0 2.3054793584184545 0.0 82-83 0.0 0.0 0.0 2.5700311954216337 0.0 84-85 0.0 0.0 0.0 2.8902573003131433 0.0 86-87 0.0 0.0 0.0 3.2906761451612425 0.0 88-89 0.0 0.0 0.0 3.749988855239969 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3025 0.0 36.159515 1 TCTACGA 55 0.0025679353 34.57705 2 CATTCCG 245 0.0 32.964764 9 TCCGCCG 30 0.0039498177 31.66967 10-11 GTATCAA 4590 0.0 29.736467 1 GTACATG 3895 0.0 27.350212 1 ACATGGG 4020 0.0 25.899248 3 TACATGG 4045 0.0 25.857992 2 TCAACGC 5370 0.0 25.319834 4 CAACGCA 5380 0.0 25.184402 5 ATCAACG 5475 0.0 24.834248 3 AACGCAG 5490 0.0 24.746723 6 TATCAAC 5760 0.0 23.689184 2 CGTACAC 125 2.2424669E-4 22.81972 3 GAGTACT 3700 0.0 21.954239 12-13 ACGCAGA 6185 0.0 21.891336 7 CGCAGAG 6215 0.0 21.861023 8 GTGTAGC 655 0.0 21.782116 1 GTAGCAC 175 4.018184E-6 21.733067 3 AGTACTT 3850 0.0 21.592422 12-13 >>END_MODULE